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ID 13423
Eprint ID
13423
フルテキストURL
Thumnail 117_41.pdf 6.36 MB
著者
阪口 義彦 岡山大学大学院医歯学総合研究科病原細菌学
抄録
Clostridium botulinum types C and D toxin production is governed by the specific bacteriophages. A type C phage,c-st,was isolated from a toxigenic strain of C-Stockholm,and its whole genome sequence was determined.The genome was a linear double-stranded 185,681bp DNA with a G+C content of 26.2%, and identical 403-bp sequences were found at each end of the genome. From the DNA research, 198 potential protein-coding regions (ORFs)were identified. The genes for type C progenitor toxin and C3 exoenzyme were identified, but no type D toxin gene was found.A number of genes were homologous to those of a Bacillus subtilis bacteriophage,SP D. On Southern blot analysis between type C and D cell DNAs and the specific probes for H and L chains of C and D neurotoxins, it became clear that type C and D strains posses only one type of toxin gene.From these findings, it is concluded that cross-neutralization observed between toxins (types C and D)and their antiserums can be caused by the common-antigens in C and D toxins.
キーワード
ボツリヌス菌 (Clostridium botulinum)
ファージ (phage)
神経毒素遺伝子 (neurotoxin gene)
備考
原著
発行日
2005-05-20
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
117巻
1号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
41
終了ページ
46
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/117/1/117_1_41/_article/-char/ja/
言語
Japanese
著作権者
Copyright© 岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma