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Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=歯周病原細菌を原因とするヒトと伴侶動物の犬における人獣共通感染症検査に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TaiMasako en-aut-sei=Tai en-aut-mei=Masako kn-aut-name=田井真砂子 kn-aut-sei=田井 kn-aut-mei=真砂子 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180927 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ベンジルイソチオシアネートの、メチル-β-シクロデキストリン及びMK571との組合せによるヒト大腸がん細胞での相乗的増殖抑制作用 kn-title=Synergistic antiproliferative effects of benzyl isothiocyanate in combination with methyl-β-cyclodextrin and MK571 in human colorectal cancer cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YangQifu en-aut-sei=Yang en-aut-mei=Qifu kn-aut-name=楊祺福 kn-aut-sei=楊 kn-aut-mei=祺福 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180927 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトがん細胞株におけるOCT4 遺伝子発現の決定的な証拠: OCT4陽性細胞集団の機能検証 kn-title=Conclusive Evidence for OCT4 Transcription in Human Cancer Cell Lines: Possible Role of a Small OCT4-Positive Cancer Cell Population en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiyamotoTomoyuki en-aut-sei=Miyamoto en-aut-mei=Tomoyuki kn-aut-name=宮本朋幸 kn-aut-sei=宮本 kn-aut-mei=朋幸 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=構成的アンドロスタン受容体活性物質モムフルオロスリンによるラット肝発がん性のヒトへのリスク評価 kn-title=Human Risk Assessment for Rat Liver Tumors Induced by a Constitutive Androstane Receptor Activator Momfluorothrin en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OkudaYu en-aut-sei=Okuda en-aut-mei=Yu kn-aut-name=奥田優 kn-aut-sei=奥田 kn-aut-mei=優 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ヒト歯肉線維芽細胞との共培養がiPS細胞の心筋細胞への成熟に与える影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MatsudaYusuke en-aut-sei=Matsuda en-aut-mei=Yusuke kn-aut-name=松田祐典 kn-aut-sei=松田 kn-aut-mei=祐典 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト骨格形成細胞分化におけるurothelial cancer-associated 1長鎖ノンコーディングRNAの生理的役割 kn-title=Physiological role of urothelial cancer-associated 1 long noncoding RNA in human skeletogenic cell differentiation en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IshikawaTakanori en-aut-sei=Ishikawa en-aut-mei=Takanori kn-aut-name=石川崇典 kn-aut-sei=石川 kn-aut-mei=崇典 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=真菌二次代謝産物(+)-terreinがAggregatibacter actinomycetemcomitans刺激時にヒト歯肉上皮細胞に及ぼす影響の解明 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakamuraArisa en-aut-sei=Nakamura en-aut-mei=Arisa kn-aut-name=中村亜里紗 kn-aut-sei=中村 kn-aut-mei=亜里紗 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20171227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=JAK阻害剤のメカニカルストレスによるヒト軟骨細胞からの蛋白分解酵素発現抑制 kn-title=Inhibitory effect of JAK inhibitor on mechanical stress-induced protease expression by human articular chondrocytes en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MachidaTakahiro en-aut-sei=Machida en-aut-mei=Takahiro kn-aut-name=町田崇博 kn-aut-sei=町田 kn-aut-mei=崇博 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol=107 cd-vols= no-issue= article-no= start-page=5 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Alzheimer’s disease : Relationship between the Alzheimer’s disease and human microbiome kn-title=アルツハイマー病の背景と発症 : ヒトマイクロバイオーム（細菌叢）との関連の視点から en-subtitle= kn-subtitle= en-abstract= kn-abstract= Alzheimer’s disease (AD) is a progressive, neurodegenerative disease characterized by memory and language disorder. The accumulation of senile plaques called β-amyloid and neurofibrillary tangles involving protein tau in the brains of AD patients have been considered as two hallmarks of AD. In AD, it is reported that accumulation of β-amyloid may be observed 25 years before onset, supporting early diagnosis and treatment by brain image analysis, because several techniques have recently been developed to detect β-amyloid and tau protein in brains of persons diagnosed with AD. AD patients are usually suffering from other diseases such as diabetes or periodontal disease, and there is accumulating data to show that these diseases associate with the human microbiome, such as gut and oral microbiota. In this report, the relation ship between AD and the human microbiome is reviewed. en-copyright= kn-copyright= en-aut-name=FujiiYusuke en-aut-sei=Fujii en-aut-mei=Yusuke kn-aut-name=藤井祐介 kn-aut-sei=藤井 kn-aut-mei=祐介 aut-affil-num=1 ORCID= en-aut-name=MoritaHidetoshi en-aut-sei=Morita en-aut-mei=Hidetoshi kn-aut-name=森田英利 kn-aut-sei=森田 kn-aut-mei=英利 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院　環境生命科学研究科 affil-num=2 en-affil= kn-affil=岡山大学大学院　環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト大腸がん細胞のベンジルイソチオシアネート耐性機構へのホスファチジルイノシトール 3-キナーゼの関与 kn-title=Involvement of phosphatidylinositide 3-kinase pathway in the resistant mechanisms against benzyl isothiocyanate in human colorectal cancer cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=LiuXiaoyang en-aut-sei=Liu en-aut-mei=Xiaoyang kn-aut-name=劉瀟陽 kn-aut-sei=劉 kn-aut-mei=瀟陽 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ミャンマーの乳癌症例ではヒト乳癌ウイルス（HMTV）の陽性率は低い kn-title=Low prevalence of human mammary tumor virus (HMTV) in breast cancer patients from Myanmar en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Thar Htet San en-aut-sei=Thar Htet San en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト腎におけるVasohibin-2の免疫組織化学的評価：　耐糖能障害と腎機能障害への意義 kn-title=Immunohistochemistry of Vasohibin-2 in Human Kidney Disease: Implication in Impaired Glucose Tolerance and Reduced Renal Function en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ArataYuka en-aut-sei=Arata en-aut-mei=Yuka kn-aut-name=荒田夕佳 kn-aut-sei=荒田 kn-aut-mei=夕佳 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト膀胱癌細胞におけるREIC/Dkk-3の強発現により、CD147の発現および細胞増殖が抑制された kn-title=Overexpression of REIC/Dkk-3 suppresses the expression of CD147 and inhibits the proliferation of human bladder cancer cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HorikawaYuhei en-aut-sei=Horikawa en-aut-mei=Yuhei kn-aut-name=堀川雄平 kn-aut-sei=堀川 kn-aut-mei=雄平 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ヒト体外受精における単一凍結融解胚盤胞移植に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=UenoSatoshi en-aut-sei=Ueno en-aut-mei=Satoshi kn-aut-name=上野智 kn-aut-sei=上野 kn-aut-mei=智 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=広視野におけるヒト物体知覚に関するfMRI研究 kn-title=fMRI Studies on the Human Visual Object Perception in a Wide Visual Field en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=郭嘉躍 kn-aut-sei=郭 kn-aut-mei=嘉躍 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Tchnology, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ブタおよびヒト小卵胞由来卵母細胞の体外成熟能改善に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OkudairaYuichi en-aut-sei=Okudaira en-aut-mei=Yuichi kn-aut-name=奥平裕一 kn-aut-sei=奥平 kn-aut-mei=裕一 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト口腔扁平上皮癌におけるタキキニン3(Tac3)受容体の分布 kn-title=Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ObataKyoichi en-aut-sei=Obata en-aut-mei=Kyoichi kn-aut-name=小畑協一 kn-aut-sei=小畑 kn-aut-mei=協一 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of synthetic (+)-terrein on secretion of proteins induced by interleukin-6 and the target molecules on signaling pathway in human gingival fibroblasts kn-title=真菌二次代謝産物である(+)-terrein がヒト歯肉線維芽細胞におけるinterleukin-6 誘導性タンパク質の産生に及ぼす影響とその標的分子の解明 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamamotoSatoshi en-aut-sei=Yamamoto en-aut-mei=Satoshi kn-aut-name=山本総司 kn-aut-sei=山本 kn-aut-mei=総司 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=突発性発疹患児の急性期から回復期における唾液中ヒトヘルペスウイルス-6および-7DNAのモニタリング kn-title=Monitoring of Human Herpesviruses-6 and -7 DNA in Saliva Samples During the Acute and Convalescent Phases of Exanthem Subitum en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiyazakiYuki en-aut-sei=Miyazaki en-aut-mei=Yuki kn-aut-name=宮崎裕樹 kn-aut-sei=宮崎 kn-aut-mei=裕樹 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=腫瘍抑制因子REIC/Dkk-3によるCD147の発現低下とヒト前立腺癌細胞増殖抑制の関連 kn-title=The Downregulation of the Expression of CD147 by Tumor Suppressor REIC/Dkk-3, and its Implication in Human Prostate Cancer Cell Growth Inhibition en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MoriAkihiro en-aut-sei=Mori en-aut-mei=Akihiro kn-aut-name=森聰博 kn-aut-sei=森 kn-aut-mei=聰博 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20161227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトRAD17コドン546における遺伝子多型は大腸癌の危険性に関与する kn-title=Human RAD 17 Polymorphism at Codon 546 Is Associated with the Risk of Colorectal Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YasudaYukiko en-aut-sei=Yasuda en-aut-mei=Yukiko kn-aut-name=保田雪子 kn-aut-sei=保田 kn-aut-mei=雪子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20161227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ウイルスが誘導するマイクロRNA-29はMCL1発現抑制を介してヒト骨肉腫細胞における化学療法抵抗性を改善する kn-title=Ablation of MCL1 expression by virally induced microRNA-29 reverses chemoresistance in human osteosarcomas en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OsakiShuhei en-aut-sei=Osaki en-aut-mei=Shuhei kn-aut-name=尾ｱ修平 kn-aut-sei=尾ｱ kn-aut-mei=修平 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20161227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=除鉄により誘起されるN-cadherin発現の抑制は、ヒト食道癌における浸潤性悪性表現型を抑制する kn-title=Iron depletion-induced downregulation of 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dt-pub-year=2016 dt-pub=20160325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=伸展刺激はヒト3次元全層培養皮膚の表皮を肥厚させ、基底膜を発達させる kn-title=Mechanical Stretch on Human Skin Equivalents Increases the Epidermal Thickness and Develops the Basement Membrane en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TokuyamaEijiro en-aut-sei=Tokuyama en-aut-mei=Eijiro kn-aut-name=ｺ山英二郎 kn-aut-sei=ｺ山 kn-aut-mei=英二郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20151231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=XV型コラーゲンはヒト肝細胞癌の発癌過程における類洞の毛細血管化に寄与する重要な病理組織学的因子である kn-title=Human collagen XV is a prominent histopathological component of sinusoidal capillarization in hepatocellular carcinogenesis en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KimuraKoji en-aut-sei=Kimura en-aut-mei=Koji kn-aut-name=木村紘爾 kn-aut-sei=木村 kn-aut-mei=紘爾 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ヒト黄体化顆粒層細胞における細胞生存性に及ぼすヒト絨毛性ゴナドトロピンの影響に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HirataRei en-aut-sei=Hirata en-aut-mei=Rei kn-aut-name=平田麗 kn-aut-sei=平田 kn-aut-mei=麗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト肺微小血管内皮細胞における、フリーラジカルスカベンジャーであるエダラボンの、サイトカイン刺激による血管透過性亢進に対する抑制効果：デキサメサゾン及び一酸化窒素合成酵素阻害剤との比較 kn-title=Inhibitory Effects of Edaravone, a Free Radical Scavenger, on Cytokine-induced　Hyperpermeability of Human Pulmonary Microvascular Endothelial Cells: A Comparison with Dexamethasone and Nitric Oxide Synthase Inhibitor en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SaitoYukie en-aut-sei=Saito en-aut-mei=Yukie kn-aut-name=齋藤有希惠 kn-aut-sei=齋藤 kn-aut-mei=有希惠 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒアルロン酸はIL-1β産生およびこれによるNF-κBの活性化を阻害することにより、ヒト軟骨細胞においてメカニカルストレスにより誘導される異化酵素の発現を抑制する kn-title=Hyaluronan suppresses mechanical stress-induced expression of catabolic enzymes by human chondrocytes 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no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ヒト歯肉線維芽細胞のリソソーム酵素カテプシンB，L分泌に対するIL-6が及ぼす影響に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=GotoAyaka en-aut-sei=Goto en-aut-mei=Ayaka kn-aut-name=後藤絢香 kn-aut-sei=後藤 kn-aut-mei=絢香 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20141231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=日本人において、ヒトRhoグアニンヌクレオチド交換因子（ARHGEF）11遺伝子は、統合失調症の発症と関連している kn-title=Human Rho guanine nucleotide exchange factor 11 gene is associated with schizophrenia in a Japanese population en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MizukiYutaka en-aut-sei=Mizuki en-aut-mei=Yutaka kn-aut-name=水木寛 kn-aut-sei=水木 kn-aut-mei=寛 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=アスコルビン酸-2グルコシド添加のUW液を用いて凍結保存した、ヒト膵島の血糖感受性インシュリン分泌能維持について kn-title=Maintenance of Glucose-sensitive Insulin Secretion of Cryopreserved Human Islets with University of Wisconsin Solution and Ascorbic Acid-2 Glucoside en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ArataTakashi en-aut-sei=Arata en-aut-mei=Takashi kn-aut-name=荒田尚 kn-aut-sei=荒田 kn-aut-mei=尚 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトの視聴覚時間統合メカニズムに関するERP研究 kn-title=Studies on Human Mechanism of Audiovisual Temporal Integration by Event-related Potential en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=楊菁　菁 kn-aut-sei=楊 kn-aut-mei=菁　菁 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=3社製品間における注射用蒸留水中の光重合開始剤の 1-hydroxycyclohexyl phenyl ketoneの定量とヒト単核球に対する細胞毒性に関する研究 kn-title=Quantitation and Human Monocyte Cytotoxicity of the Polymerization Agent 1-Hydroxycyclohexyl Phenyl Ketone (Irgacure 184) from Three Brands of Aqueous Injection Solution en-subtitle= kn-subtitle= en-abstract= kn-abstract= 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kn-aut-mei=太一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト口腔癌細胞HSC-4における5-アミノレブリン酸を用いた光線力学的治療法の細胞障害作用の改善 kn-title=Improvement of the Efficacy of 5-aminolevulinic Acid-mediated Photodynamic Treatment in Human Oral Squamous Cell Carcinoma HSC-4 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamamotoMasanao en-aut-sei=Yamamoto en-aut-mei=Masanao kn-aut-name=山本昌直 kn-aut-sei=山本 kn-aut-mei=昌直 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120927 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト癌細胞におけるトラスツヅマブ耐性機構の解析とHER2細胞外ドメイン発現によるADCC活性増強を介した感受性誘導 kn-title=Mechanism of resistance to trastuzumab and molecular sensitization via ADCC activation by exogenous expression of HER2-extracellular domain in human cancer cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YoshidaRyosuke en-aut-sei=Yoshida en-aut-mei=Ryosuke kn-aut-name=吉田亮介 kn-aut-sei=吉田 kn-aut-mei=亮介 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=3 article-no= start-page=231 end-page=238 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20121203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=HuH-7 cell line established from a highly differentiated human hepatocellular carcinoma kn-title=高分化型ヒト肝癌由来細胞株“HuH-7” en-subtitle= kn-subtitle= en-abstract= kn-abstract=高分化型ヒト肝癌由来細胞株“HuH-7”は，1982年にCancer Researchにその樹立を報告した．HuH-7は，当時の岡山大学医学部附属癌源研究施設病理部門（故佐藤二郎教授）の下で樹立し，これまで多くの研究分野で利用され，世界的に有名な肝癌細胞株となっている．本稿では，有用性の高い分化機能を有するヒト肝癌細胞株HuH-7について，肝細胞癌の腫瘍マーカーであるα-fetoprotein（AFP）を中心に，この細胞株を用いた研究分野に関する詳細を紹介する． en-copyright= kn-copyright= en-aut-name=NakabayashiHidekazu en-aut-sei=Nakabayashi en-aut-mei=Hidekazu kn-aut-name=中林秀和 kn-aut-sei=中林 kn-aut-mei=秀和 aut-affil-num=1 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name=武田和久 kn-aut-sei=武田 kn-aut-mei=和久 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=北海道情報大学　医療情報学科 affil-num=2 en-affil= kn-affil=介護老人保健施設　仁和の里 en-keyword=肝細胞癌 kn-keyword=肝細胞癌 en-keyword=培養細胞 kn-keyword=培養細胞 en-keyword=α-フェトプロテイン kn-keyword=α-フェトプロテイン en-keyword=HuH-7 kn-keyword=HuH-7 END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=2 article-no= start-page=105 end-page=110 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Preclinical evaluation of telomerase-specific oncolytic virotherapy for human bone and soft tissue sarcomas kn-title=テロメラーゼ依存性腫瘍融解ウイルス療法の骨・軟部肉腫に対する前臨床的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=骨・軟部肉腫は, 一部に治療抵抗性で予後の悪い症例が存在するため, 新たな治療法の確立が重要な課題である.　我々は, 5型アデノウイルスを基本骨格として, テロメラーゼ活性に依存して増殖する腫瘍融解ウイルス（OBP-301）や, coxsackie and adenovirus receptor（CAR）陰性の腫瘍細胞に感染するファイバー改変型ウイルス（OBP-405）を用い, 骨・軟部肉腫細胞に対する抗腫瘍効果を検討した.　 　14種類の骨・軟部肉腫細胞株に対してOBP-301の細胞障害活性を検討し, 12種類の細胞株でOBP-301に感受性を認めた.　また, OBP-301の細胞障害活性はCARの発現と相関していた.　さらに, テロメラーゼ活性の低い細胞に対しても, 5型アデノウイルスの複製に必須のE1Aによりテロメラーゼ活性の増強効果がおこり, 強い抗腫瘍活性を示すことを明らかにした.　次に, 骨肉腫脛骨同所性移植動物モデルを作成しOBP-301を投与したところ, OBP-301投与群では対象群と比べて有意に腫瘍増殖を抑制した.　最後に, OBP-301に感受性を認めなかったCAR陰性細胞株に対してOBP-405を用いて検討し, OBP-405が有効に作用することを確認した.　 　OBP-301やOBP-405を用いたウイルス療法は, 骨・軟部肉腫に対する新たな治療法となる可能性がある.　 en-copyright= kn-copyright= en-aut-name=SasakiTsuyoshi en-aut-sei=Sasaki en-aut-mei=Tsuyoshi kn-aut-name=佐々木剛 kn-aut-sei=佐々木 kn-aut-mei=剛 aut-affil-num=1 ORCID= en-aut-name=TazawaHiroshi en-aut-sei=Tazawa en-aut-mei=Hiroshi kn-aut-name=田澤大 kn-aut-sei=田澤 kn-aut-mei=大 aut-affil-num=2 ORCID= en-aut-name=HaseiJo en-aut-sei=Hasei en-aut-mei=Jo kn-aut-name=長谷井嬢 kn-aut-sei=長谷井 kn-aut-mei=嬢 aut-affil-num=3 ORCID= en-aut-name=KunisadaToshiyuki en-aut-sei=Kunisada en-aut-mei=Toshiyuki kn-aut-name=国定俊之 kn-aut-sei=国定 kn-aut-mei=俊之 aut-affil-num=4 ORCID= en-aut-name=YoshidaAki en-aut-sei=Yoshida en-aut-mei=Aki kn-aut-name=吉田晶 kn-aut-sei=吉田 kn-aut-mei=晶 aut-affil-num=5 ORCID= en-aut-name=HashimotoYuuri en-aut-sei=Hashimoto en-aut-mei=Yuuri kn-aut-name=橋本悠里 kn-aut-sei=橋本 kn-aut-mei=悠里 aut-affil-num=6 ORCID= en-aut-name=YanoShuya en-aut-sei=Yano en-aut-mei=Shuya kn-aut-name=矢野修也 kn-aut-sei=矢野 kn-aut-mei=修也 aut-affil-num=7 ORCID= en-aut-name=YoshidaRyosuke en-aut-sei=Yoshida en-aut-mei=Ryosuke kn-aut-name=吉田亮介 kn-aut-sei=吉田 kn-aut-mei=亮介 aut-affil-num=8 ORCID= en-aut-name=UnoFutoshi en-aut-sei=Uno en-aut-mei=Futoshi kn-aut-name=宇野太 kn-aut-sei=宇野 kn-aut-mei=太 aut-affil-num=9 ORCID= en-aut-name=KagawaShunsuke en-aut-sei=Kagawa en-aut-mei=Shunsuke kn-aut-name=香川俊輔 kn-aut-sei=香川 kn-aut-mei=俊輔 aut-affil-num=10 ORCID= en-aut-name=MorimotoYuki en-aut-sei=Morimoto en-aut-mei=Yuki kn-aut-name=森本裕樹 kn-aut-sei=森本 kn-aut-mei=裕樹 aut-affil-num=11 ORCID= en-aut-name=UrataYasuo en-aut-sei=Urata en-aut-mei=Yasuo kn-aut-name=浦田泰生 kn-aut-sei=浦田 kn-aut-mei=泰生 aut-affil-num=12 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name=藤原俊義 kn-aut-sei=藤原 kn-aut-mei=俊義 aut-affil-num=13 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name=尾ｱ敏文 kn-aut-sei=尾ｱ kn-aut-mei=敏文 aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　整形外科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　整形外科学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　整形外科学 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　整形外科学 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=8 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=9 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=10 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=11 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　整形外科学 affil-num=12 en-affil= kn-affil=オンコリスバイオファーマ affil-num=13 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=14 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　整形外科学 en-keyword=アデノウイルス kn-keyword=アデノウイルス en-keyword=肉腫 kn-keyword=肉腫 en-keyword=ALT kn-keyword=ALT en-keyword=ヒトテロメラーゼ逆転写酵素 kn-keyword=ヒトテロメラーゼ逆転写酵素 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ヒトUDP-グルクロン酸転移ヒトUDP-グルクロン酸転移酵素UGT2B7の触媒機能におけるN-結合型糖鎖修飾の役割 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NagaokaKenjiro en-aut-sei=Nagaoka en-aut-mei=Kenjiro kn-aut-name=長岡憲次郎 kn-aut-sei=長岡 kn-aut-mei=憲次郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=化学物質の光毒性・光遺伝毒性試験における培養ヒトケラチノサイトの有用性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HorinouchiMayumi en-aut-sei=Horinouchi en-aut-mei=Mayumi kn-aut-name=堀ノ内眞弓 kn-aut-sei=堀ノ内 kn-aut-mei=眞弓 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Stage-specific embryonic antigen-4を用いたヒト乳歯歯根膜幹細胞の同定 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=FukushimaHiroaki en-aut-sei=Fukushima en-aut-mei=Hiroaki kn-aut-name=福島宏明 kn-aut-sei=福島 kn-aut-mei=宏明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Eosinophil Cationic Proteinが正常ヒト線維芽細胞に及ぼす影響に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SatoTakamaro en-aut-sei=Sato en-aut-mei=Takamaro kn-aut-name=佐藤公麿 kn-aut-sei=佐藤 kn-aut-mei=公麿 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=活性型Matrix metalloproteinase-3がヒト単球系細胞株THP-1における可溶型インターロイキン-6受容体の産生に及ぼす影響に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KobayashiHiroya en-aut-sei=Kobayashi en-aut-mei=Hiroya kn-aut-name=小林寛也 kn-aut-sei=小林 kn-aut-mei=寛也 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=周期的伸張負荷がヒト前十字靭帯細胞のCCN2/CTGF発現を活性化する kn-title=Mechanical stretch increases CCN2/CTGF expression in anterior cruciate ligament-derived cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiyakeYoshiaki en-aut-sei=Miyake en-aut-mei=Yoshiaki kn-aut-name=三宅由晃 kn-aut-sei=三宅 kn-aut-mei=由晃 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=TP53変異の有無が異なるヒトのリンパ芽球様細胞株間における5-fluorouracilに対する遺伝毒性及び遺伝子発現への反応性の差 kn-title=Different responses to 5-fluoraouracil in mutagenicity and gene expression between two human lymphoblastoid cell lines with or without TP53 mutation en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OkaHiroaki en-aut-sei=Oka en-aut-mei=Hiroaki kn-aut-name=岡宏昭 kn-aut-sei=岡 kn-aut-mei=宏昭 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト悪性グリオーマの浸潤と血管新生を模倣する新規グリオーマ動物モデルの検討 kn-title=Novel animal glioma models that separately exhibit two different invasive and angiogenic phenotypes of human glioblastomas en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InoueSatoshi en-aut-sei=Inoue en-aut-mei=Satoshi kn-aut-name=井上智 kn-aut-sei=井上 kn-aut-mei=智 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=32 cd-vols= no-issue=4 article-no= start-page=365 end-page=372 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=1990 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Assay of GBM antigen in urine and serum with an anti-human renal monoclonal antibody kn-title=抗ヒト腎モノクローナル抗体を用いた尿中及び血中のGBM抗原の測定 第2編 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using a monoclonal antibody (Mab-G3) recognizing glomerular basement membrane (GBM), we assayed GBM antigen (G3-Ag) in the urine and serum of renal disease patients by sandwich ELISA. The subjects included normal control (NOR), minimal change nephrotic syndrome (MCNS), IgA nephropathy (IgA), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN) and chronic renal failure (CRF). The urine and serum was used as the material. With urinary G3-Ag, there were no statistically significant differences among the NOR, MCNS, IgA, MN, MPGN and CRF groups. Although no correlation was observed with proteinuria, hematuria, serum creatinine, serum β2 microglobulin and urinary NAG, urinary G3-Ag showed a significant (p<0.05) increase in excretion in the group of progressive CRF patients with s-Cr more than 1.0 mg/dl/month as compared to the stationary CRF group with s-Cr<1.0 mg/dl/month. Serum G3-Ag showed lower values in almost all cases, and there were no significant differences among the renal disease groups. The above findings led us to believe that the assay of urinary G3-Ag was useful in determining the degree of GBM disorder. It was also presumed that assay of renal antigens in urine and serum with the respective anti-human renal monoclonal antibodies could be a new tool in diagnosing renal diseases. en-copyright= kn-copyright= en-aut-name=MinoYasuaki en-aut-sei=Mino en-aut-mei=Yasuaki kn-aut-name=味埜泰明 kn-aut-sei=味埜 kn-aut-mei=泰明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第三内科 en-keyword=monoclonal antibody kn-keyword=monoclonal antibody en-keyword=sandwich ELISA kn-keyword=sandwich ELISA en-keyword=GBM antigen kn-keyword=GBM antigen en-keyword=renal disease kn-keyword=renal disease END start-ver=1.4 cd-journal=joma no-vol=32 cd-vols= no-issue=4 article-no= start-page=353 end-page=364 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=1990 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study on the change of glomerular antigenicity in various renal diseases with anti-human renal monoclonal antibodies kn-title=抗ヒト腎モノクローナノレ抗体を用いた疾患腎における抗原性の変化の検討 第1編 en-subtitle= kn-subtitle= en-abstract= kn-abstract=We produced 22 different kinds of monoclonal antibody (Mab) by immunizing mice with human GBM antigens. In these Mabs, Mab-G1 to G5 recognized only GBM in the glomerulas, Mab-E1 and E2 recognized only glomerular epithelial cells, and Mab-M1 to M4 recognized mainly mesangium. The reactions of these Mabs with known GBM antigens such as type IV collagen, fibronectin and laminin were negative by immunoblotting. Using Mab-G1, Mab-E1 and Mab-M1, changes in the antigenicity of antigens recognized by Mabs were examined on kidney sections from the patients with various renal diseases by the indirect immunofluorescence test. When Mab-G1 recognizing GBM was used, there was no particular change of anti-genicity in minimal change nephrotic syndrome (MCNS) and IgA nephropathy (IgA), whereas in membranous nephropathy (MN) thickened GBM was found to maintain anti-genicity and the region of deposits was observed as negative punched-out region. In type I and III of membranoproliferative glomerulonephritis (MPGN), GBM was observed only outside of subendothlial deposits without showing double contour. In type II MPGN, GBM showed a double linear pattern and antigenicity of GBM in regions of dense deposits was not detected. When Mab-E1 recognizing glomerular epithelial cells was used, there was no change of antigenicity in the renal diseases. Further, in crescentic glomerulone-phritis, the region of the cellular crescents was not stained, When Mab-M1 recognizing mesangium was used, extensive staining was observed in the increased mesangium in IgA, MPGN, and diabetic nephropathy. We feel that it is of significance in elucidating the pathogenesis of renal diseases to study the changes of glomerular antigenicity in diseased kidneys by using anti-human renal monoclonal antibodies. en-copyright= kn-copyright= en-aut-name=MinoYasuaki en-aut-sei=Mino en-aut-mei=Yasuaki kn-aut-name=味埜泰明 kn-aut-sei=味埜 kn-aut-mei=泰明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第三内科 en-keyword=monoclonal antibody kn-keyword=monoclonal antibody en-keyword=human kidney antigen kn-keyword=human kidney antigen en-keyword=GBM kn-keyword=GBM en-keyword=mesangium kn-keyword=mesangium en-keyword=glomerular epithelial cell kn-keyword=glomerular epithelial cell END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20111231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト変形性関節症軟骨におけるHigh-mobility Group Box Chromosomal Protein-1(HMGB-1)の発現と局在 kn-title=Gene Expression and Localization of High-mobility Group Box Chromosomal Protein-1 (HMGB-1) in Human Osteoarthritic Cartilage en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TeradaChuji en-aut-sei=Terada en-aut-mei=Chuji kn-aut-name=寺田忠司 kn-aut-sei=寺田 kn-aut-mei=忠司 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=樹状細胞サブセットとヒト乳頭腫ウイルス関連皮膚病変での免疫環境 kn-title=Dendritic cell subsets and immunological milieu in inflammatory human papilloma virus-related skin lesions en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakayamaYumi en-aut-sei=Nakayama en-aut-mei=Yumi kn-aut-name=中山由美 kn-aut-sei=中山 kn-aut-mei=由美 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=テロメラーゼ依存的腫瘍融解アデノウイルス製剤によるDNA修復阻害を介したヒト癌細胞の放射線感受性増強作用 kn-title=Telomerase-Dependent Oncolytic Adenovirus Sensitizes Human Cancer Cells to Ionizing Radiation via Inhibition of DNA Repair Machinery en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KurodaShinji en-aut-sei=Kuroda en-aut-mei=Shinji kn-aut-name=黒田新士 kn-aut-sei=黒田 kn-aut-mei=新士 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト大腸癌細胞株SW620におけるCD133陽性細胞の特性 kn-title=Characteristics of CD133(+) human colon cancer SW620 cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawamotoHironobu en-aut-sei=Kawamoto en-aut-mei=Hironobu kn-aut-name=河本洋伸 kn-aut-sei=河本 kn-aut-mei=洋伸 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=人工骨補填材(MASTERGRAFT? Granules)を用いて治療を行ったヒト抜歯窩の組織学的評価−基礎研究− kn-title=Histological evaluation of human alveolar sockets treated with artificial bone substitute material(MASTERGRAFT? Granules).-A preliminary study- en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WakimotoMari en-aut-sei=Wakimoto en-aut-mei=Mari kn-aut-name=脇本真理 kn-aut-sei=脇本 kn-aut-mei=真理 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=機能的核磁気共鳴画像法を用いたヒトの広視野の視覚メカニズムに関する研究 kn-title=Studies on the Human Visual Mechanism of Wide-field by Functional Magnetic Resonance Imaging en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=閻天翼 kn-aut-sei=閻 kn-aut-mei=天翼 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20101231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト固形癌に対する、作用機序の異なる2剤併用ウイルス療法の前臨床評価 kn-title=Preclinical Evaluation of Differentially Targeting Dual Virotherapy for Human Solid Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakaiRyo en-aut-sei=Sakai en-aut-mei=Ryo kn-aut-name=酒井亮 kn-aut-sei=酒井 kn-aut-mei=亮 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトリンパ球混合培養反応における、AGE誘導性接着分子発見、サイトカイン産生、リンパ球増殖に対するヒスタミンの抑制効果の検討 kn-title=Histamine inhibits adhesion molecule expression in human monocytes, induced by advanced glycation end products, during the mixed lymphocyte reaction en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ZhangJiyong en-aut-sei=Zhang en-aut-mei=Jiyong kn-aut-name=張継勇 kn-aut-sei=張 kn-aut-mei=継勇 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=6-2 article-no= start-page=3187 end-page=3191 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590515 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Free Amino-Acids in the Brain (X) on the free amino-acids and related substances in various parts in the mature and foetal human brains kn-title=脳の遊離アミノ酸について(X) 成熟ヒト脳およびヒト胎児脳各部位における遊離アミノ酸およびその関連物質について en-subtitle= kn-subtitle= en-abstract=With the brains of foetuses on the fifth gravid month and on the eighth gravid month the author isolated and estimated the free amino-acids and related substances at various parts of these brains by means of the ion-exchange chromatography, and conducted the same investigations with the normal tissues surrounding the tumor of the frontal lobe in the brain of an 18-year-old patient with tubosclerosis. 1. The quantities of free amino-acids in the foetal brains differ significantly from those in the mature human brain. 2. In the foetal brain, with some variations according to sites, amino-acids such as taurine, phosphoethanolamine, glycine and alanine, generally tend to decrease with the advance in age, while glutaminic, aspartic and γ-amino-butyric acids tend to increase. 3. In either one of the foetal brains γ-amino-butyric acid is detected in a largest quantity in the hypothalamus. 4. Cystathionine which is often detected in a large quantity in the human brain after birth can hardly be recognized in the foetal brain. kn-abstract=イオン交換クロマトグラフイーをもちいて,妊娠5ケ月および8ケ月胎児脳の各部位ならびに18才結節性硬化症患者脳の前頭葉,腫瘍周辺の非結節性組織について遊離アミノ酸およびその関連物質を各々分離定量した. 1) 妊娠5ケ月なうびに8ケ月胎児脳と成熟ヒト脳を比較すると,胎児脳と生後ヒト脳との間にはその遊離アミノ酸量にかなり著明な差異を認める. 2) 胎児脳では,脳各部位ともに多少の例外はあるが,生育の段階につれてタウリン,ホスホエタノラミン,グリシン,アラニン等は減少し,グルタミン酸,アスパラギン酸, γ-アミノ酪酸等は増加する傾向を示している. 3) γ-アミノ酪酸は胎児脳のいずれでも,視床下部で最高値を示した. 4) 生後のヒト脳で多量に測定され得るシスタチオニンは胎児脳にはほとんど存在しない. en-copyright= kn-copyright= en-aut-name=FukaiNobuhiro en-aut-sei=Fukai en-aut-mei=Nobuhiro kn-aut-name=深井延浩 kn-aut-sei=深井 kn-aut-mei=延浩 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=4-1 article-no= start-page=1591 end-page=1594 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Free Amino Acids in the Brain Part VII Estimation of Free Amino Acids in the Human Brain by the Ion-Exchange Chromatography kn-title=脳の遊離アミノ酸について (VII) イオン交換クロマトグラフイーによるヒト脳遊離アミノ酸の定量 en-subtitle= kn-subtitle= en-abstract= kn-abstract=By means of the ion-exchange chromatography free amino acids and their related compounds in the fresh brain tissue have been estimated. As for the test material, pieces of the cerebral cortex sectioned at the time of the surgical operation in the patients with cerebral tumor or with epilepsy are used. As the result we have obtained the following notworthy differences when compared with the results previously reported by us concerning several species of mammalians. They are: (1) In the human brain there is a conciderable amount of cystathionine. Namely, 19.4 to 43.2mg/100g wet weight, and this substance is far smaller or not recognizable in other animals; and (2) the amounts of γ-aminobutric acid and taurine are a good deal smaller than animals. Moreover, in general the pattern of the free amino acids in the human cerebral cortex may be said to resemble closely that in dog. en-copyright= kn-copyright= en-aut-name=YunoueShigeru en-aut-sei=Yunoue en-aut-mei=Shigeru kn-aut-name=湯之上茂 kn-aut-sei=湯之上 kn-aut-mei=茂 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=687 end-page=698 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and characterization of HBs antigen from hepatoma huGK-14 cell line kn-title=ヒト培養肝癌細胞の産生するHBs抗原の精製方法とその物理化学的特性について en-subtitle= kn-subtitle= en-abstract= kn-abstract=HBs antigen was purified from the culture fluid of hepatoma huGK-14 cell line and its physico-chemical properties were studied. The purification consists of following steps: concentration of culture fluid by membrane filtration, affinity column chromatography (anti-HBs monoclonal antibody column and anti-human serum albumin antibody column), and ultracentrifugation (isopycnic centrifugation in CsCl density gradient and rate zonal centrifugation on sucrose gradient). Highly purified (purity>99%) HBs antigen was isolated with an overall yield of about 40%. The HBs antigen showed uniform spherical particles (diameter: 23.2±2.9nm) and had a specific gravity of 1.20g/cm3. The purified HBs antigen yielded, in SDS-PAGE (under reducing conditions), four protein bands with apparent molecular weights of 22,000 and 26,000 (the two major bands), and 44,000 and 47,000. The two proteins of molecular weights of 26,000 and 47,000 are likely to be glycosylated, as these were several fold reduced when the cells were cultured in the presence of Tunicamycin. Amino acid analysis, Edman degradation, carboxypeptidase digestion, and ultraviolet absorption spectrum indicated that the HBs antigen from hepatoma cells is very similar to that derived from human plasma. en-copyright= kn-copyright= en-aut-name=OdaMunehiro en-aut-sei=Oda en-aut-mei=Munehiro kn-aut-name=小田宗宏 kn-aut-sei=小田 kn-aut-mei=宗宏 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源病理学教室 en-keyword=Hepatoma kn-keyword=Hepatoma en-keyword=Cell culture kn-keyword=Cell culture en-keyword=HBs antigen kn-keyword=HBs antigen en-keyword=Purification kn-keyword=Purification en-keyword=Characterization kn-keyword=Characterization END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=589 end-page=602 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Abnormality of dominant oncogenes and recessive oncogenes in human bone and soft tissue tumors kn-title=ヒト骨・軟部腫瘍における優性および劣性癌遺伝子の変異に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Osteosarcoma is a malignant bone tumor which usually occurs in the metaphysis of long bones in adolescents or young adults, but the mechanism of disease occurrence is unknown. To examine alterations in the dominant oncogenes in the osteosarcoma and other bone and soft tissue tumors, DNA extracted from 12 bone tumors, 12 soft part tumors and 2 cells lines was hybridized with c-oncogene (c-myc, c-sis, c-raf-1, c-fos, K-ras2, c-erbB, c-fms, c-fos). DNA extracted from the same samples was examined with Rb cDNA (p0.9R, p3.8R) probes, the retinoblastoma gene (Rb gene) localized at 13q14, to analize whether a recessive mutation is a target in osteosarcoma, and other bone and soft tissue tumors. Amplification of c-myc was observed in 3 cases from 7 osteosarcomas and abnormalities in structure of the Rb gene were found in 4 from 7 osteosarcomas, 2 from 4 MFH, and 1 from 2 Ewing's sarcomas. Both amplification of the c-myc and abnormalities of the Rb gene were observed in 3 osteosarcoma cases. The results indicated that both the Rb gene and c-myc oncogene may be involved in the initiation of osteosarcoma. en-copyright= kn-copyright= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name=尾崎敏文 kn-aut-sei=尾崎 kn-aut-mei=敏文 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属癌源研究施設生化学研究部門 en-keyword=骨・軟部腫瘍 kn-keyword=骨・軟部腫瘍 en-keyword=優性癌遺伝子 kn-keyword=優性癌遺伝子 en-keyword=劣性癌遺伝子 kn-keyword=劣性癌遺伝子 en-keyword=Rb遺伝子 kn-keyword=Rb遺伝子 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=581 end-page=588 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of the treatment of pulmonary epidermoid carcinoma Part 2. Thermochemotherapy in human epidermoid carcinoma cells in culture kn-title=肺扁平上皮癌の治療に関する研究 第2編 ヒト肺扁平上皮癌細胞株を用いた温熱化学療法に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effectiveness of hyperthermia in combination with anticancer drugs on EBC-1 cell line established from a patient with pulmonary epidermoid carcinoma was investigated. Anticancer drugs tested in the present study were adriamycin, bleomycin, cisdichlorodiammineplatinum (II) and mitomycin C. EBC-1 cells were incubated with the drug for one hour at 37°C or at elevated temperature (41°C, 42°C and 43°C). The enhancement of cytotoxicity by hyperthermia was found in combination with all of the drugs. The degree of enhanced cytotoxicity was positively elated to the temperature in combinations with adriamycin and bleomycin. Especially, a synergic enhancement of cytotoxicity was found with the combination of hyperthermia and bleomycin. The present study showed that the combination of hyperthermia and anticancer drugs produced potential cytotoxicity on EBC-1 cells in vitro, and may provide useful information for the clinical trials in the future. en-copyright= kn-copyright= en-aut-name=SetoTakumi en-aut-sei=Seto en-aut-mei=Takumi kn-aut-name=瀬戸匠 kn-aut-sei=瀬戸 kn-aut-mei=匠 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=human lung cancer cell line kn-keyword=human lung cancer cell line en-keyword=thermochemotherapy kn-keyword=thermochemotherapy END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=573 end-page=580 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of the treatment of pulmonary epidermoid carcinoma Part 1. Sensitivity to various anticancer drugs against human pulmonary epidermoid carcinoma xenograft in the hamster kn-title=肺扁平上皮癌の治療に関する研究 第1編 ハムスター移植ヒト肺扁平上皮癌細胞株に対する各種制癌剤の感受性について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Chemotherapy is the only therapeutic modality applicable to patients with advanced pulmonary epidermoid carcinoma (PEC). However, the results of chemotherapy to PEC remain unsatisfactory. It is very important to have an accurate knowledge of the sensitivity of anticancer drugs against PEC in order to establish a successful chemotherapy. The in vivo sensitivity of 12 anticancer drugs was investigated using PEC cell line (EBC-1) xenografts in hamsters. In the present study, adriamycin, ifosphamide, mitomycin C, methotrexate and cisdichlorodiammineplatinum (II) showed antitumor activity against EBC-1 cells, but the other 6 drugs (ACNU, bleomycin, 5-fluorouracil, neocarzinostatin, procarbazine and vincristine) have no antitumor activity against EBC-1 cells. These results suggest that combination chemotherapy with 3 or 4 drugs with antitumor activity in the present study may be effective to PEC. en-copyright= kn-copyright= en-aut-name=SetoTakumi en-aut-sei=Seto en-aut-mei=Takumi kn-aut-name=瀬戸匠 kn-aut-sei=瀬戸 kn-aut-mei=匠 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=human lung cancer cell line kn-keyword=human lung cancer cell line en-keyword=xenograft kn-keyword=xenograft en-keyword=chemosensitivity test kn-keyword=chemosensitivity test END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=557 end-page=571 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Molecular cloning and structural analyses of human DNA sequences related to the squirrel monkey retrovirus LTR kn-title=リスザルレトロウイルスLTR関連ヒト遺伝子の分子クローニングと構造解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sequences related to the squirrel monkey retrovirus (SMRV) LTR were isolated from a genomic library of human DNA by screening under conditions of relaxed stringency. The probe contains several transcriptional signals and its regulatory sequences in SMRV-H LTR, such as the enhancer sequence, CAT box, TATA box, and polyadenylation signal. More than 50 positive signals were detected in 4.4×105 recombinant phages. The cloned human sequences named SRH strongly hybridized with SMRV-H LTR and some of them weakly hybridized with SMRV-H structural genes. In SRH-1, the region related to SMRV-H prt-pol-env was flanked by the two separately located sequences related to the LTR. SRH-3 has a solitary LTR-related region, whereas, in SRH-5, the LTR-related region was adjacent to the pol-env-related sequence. The reliability of these hybridization experiments was confirmed by reciprocal hybridization. The restriction enzyme cleavage maps of these clones were different from those of known human endogenous retroviruses. Alu sequences, a human highly repetitive sequences, were inserted to the LTR-and pol-env-related regions in SRH-5. en-copyright= kn-copyright= en-aut-name=NagaoKazutaka en-aut-sei=Nagao en-aut-mei=Kazutaka kn-aut-name=長尾一孝 kn-aut-sei=長尾 kn-aut-mei=一孝 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=ヒト内在性レトロウイルス kn-keyword=ヒト内在性レトロウイルス en-keyword=リスザルレトロウイルス kn-keyword=リスザルレトロウイルス en-keyword=Long terminal repeat kn-keyword=Long terminal repeat en-keyword=分子クローニング kn-keyword=分子クローニング END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=459 end-page=471 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Immunoelectron microscopic studies of human immunodeficiency virus antigens kn-title=ヒト免疫不全ウイルス抗原の免疫電子顕微鏡的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Immunoelectron microscopic observations of human immunodeficiency virus (HIV) antigens were made using the indirect peroxidase-labeled antibody method with monoclonal antibodies (MoAb) to the envelope glycoprotein (gp160/120), matrix protein (MA, p17), and capsid protein (CA, p24). Antigens in the virions and HIV-infected cells were detected in frozen-sectioned specimens. Positive immunoreaction of MoAb to gp160/120 was located on the viral envelope, plasma membrane, endoplasmic reticulum, and nuclear membrane. The reaction of MoAb to p17 was at the inner leaflet of the viral envelope and the nucleoid. The reaction of MoAb to p24 was localized on and around the viral core. In cell suspension, however, positive immunostaining was observed only on the surface of the viral envelope and plasma membrane with MoAb to gp160/120, and no positive staining was observed with MoAb to p17 and MoAb to p24. The reaction of HIV-infected cells with normal control serum and all of the reactions in uninfected cells with these MoAbs were negative. It appears that antibodies have difficulty penetrating into virions and cells in cell suspension. en-copyright= kn-copyright= en-aut-name=EndoSatoru en-aut-sei=Endo en-aut-mei=Satoru kn-aut-name=遠藤悟 kn-aut-sei=遠藤 kn-aut-mei=悟 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=ヒト免疫不全ウイルス kn-keyword=ヒト免疫不全ウイルス en-keyword=HIV抗原 kn-keyword=HIV抗原 en-keyword=構造蛋白質 kn-keyword=構造蛋白質 en-keyword=免疫電顕 kn-keyword=免疫電顕 en-keyword=酵素抗体法 kn-keyword=酵素抗体法 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=423 end-page=435 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Detection of human sequence related to the squirrel monkey retrovirus kn-title=ヒトゲノムDNA中のリスザルレトロウイルス関連遺伝子の検出 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Squirrel monkey retrovirus (SMRV) is an endogenous type D retrovirus of the squirrel monkey, a New World primate. Southern hybridization with cloned SMRV-H revealed that 3040 copies of SMRV proviral DNA are present in the squirrel monkey genome and the majority have almost the same physical map as that of the cloned SMRV-H. SMRV-related sequences in the human genome were sought using the same method with various cloned SMRV-H DNA fragments under conditions of relaxed stringency. The discrete restriction fragments were frequently detected in the DNA from normal humans with the LTR and parts of gag and env as probes. Since SMRV LTR has very little homology with the LTRs of other retroviruses, the fragments detected with the LTR probe were characterized as SMRV-related human sequences. SMRV LTR-related sequences were also detected in the African green monkey and chicken, but not in the salmon, mouse, or dog. In conclusion, SMRV-related sequences are present in human DNA, and some of them might represent endogenous retroviral sequences of human DNA. en-copyright= kn-copyright= en-aut-name=AsonumaHirohiko en-aut-sei=Asonuma en-aut-mei=Hirohiko kn-aut-name=阿曽沼裕彦 kn-aut-sei=阿曽沼 kn-aut-mei=裕彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=ヒトDNA kn-keyword=ヒトDNA en-keyword=内在性レトロウイルス kn-keyword=内在性レトロウイルス en-keyword=リスザルレトロウイルス kn-keyword=リスザルレトロウイルス en-keyword=サザンハイブリダイゼーション kn-keyword=サザンハイブリダイゼーション END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=3-4 article-no= start-page=285 end-page=293 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Analysis of cellular oncogenes in human bone and soft tissue tumors kn-title=ヒト骨・軟部腫瘍組織における癌遺伝子の解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The amplification and rearrangement of five cellular oncogenes (c-myc, c-K-ras, c-fos, c-raf-1, and N-myc) were studied by Southern hybridization in fourteen human bone and soft tissue tumors obtained at surgery. Amplification of c-myc was detected in the two of four osteosarcomas and one of two malignant fibrous histiocytomas. The c-myc genes in these tissues were amplified 4- to 8-fold in comparision with the placenta DNA. One of these osteosarcomas had 16- to 32-fold amplification of c-raf-1 gene without rearrangement. The clinical course of osteosarcoma and malignant fibrous histiocytoma with the amplified c-myc or c-raf-1 gene showed a rapid malignant progress with lung or bone metastasis. There appears to be a correlation between clinical prognosis and oncogene amplification. en-copyright= kn-copyright= en-aut-name=SumiiHiroshi en-aut-sei=Sumii en-aut-mei=Hiroshi kn-aut-name=住居広士 kn-aut-sei=住居 kn-aut-mei=広士 aut-affil-num=1 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name=井上一 kn-aut-sei=井上 kn-aut-mei=一 aut-affil-num=2 ORCID= en-aut-name=ItoShiro en-aut-sei=Ito en-aut-mei=Shiro kn-aut-name=伊藤士郎 kn-aut-sei=伊藤 kn-aut-mei=士郎 aut-affil-num=3 ORCID= en-aut-name=TanabeGozo en-aut-sei=Tanabe en-aut-mei=Gozo kn-aut-name=田辺剛造 kn-aut-sei=田辺 kn-aut-mei=剛造 aut-affil-num=4 ORCID= en-aut-name=TakechiHideo en-aut-sei=Takechi en-aut-mei=Hideo kn-aut-name=武智秀夫 kn-aut-sei=武智 kn-aut-mei=秀夫 aut-affil-num=5 ORCID= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name=池田正五 kn-aut-sei=池田 kn-aut-mei=正五 aut-affil-num=6 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name=小田琢三 kn-aut-sei=小田 kn-aut-mei=琢三 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部整形外科学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部整形外科学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部整形外科学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部整形外科学教室 affil-num=5 en-affil= kn-affil=吉備高原医療リハビリテーションセンター affil-num=6 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 affil-num=7 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=Oncogene kn-keyword=Oncogene en-keyword=Gene amplification kn-keyword=Gene amplification en-keyword=c-myc kn-keyword=c-myc en-keyword=c-raf-1 kn-keyword=c-raf-1 en-keyword=Osteosarcoma kn-keyword=Osteosarcoma en-keyword=MFH kn-keyword=MFH END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=1-2 article-no= start-page=131 end-page=139 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Primary culture of human primary liver cancer tissues treated with or without transcatheter arterial embolization and establishment of a cell line kn-title=肝動脈塞栓術施行或は非施行ヒト肝癌組織の初代培養と株化 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Primary culture of human primary liver cancer tissues treated with or without trancatheter arterial embolization (TAE) was perfomed with the following results. 1. The yield and viability were very low in cells from primary liver cancer tissues dissociated with enzymes. 2. Epithelial-like cells were found from TAE-treated cancer tissues at a ratio of 1/8 in both monolayer and explant culture and from TAE-nontreated tissues at a ratio of 4/12 in monolayer culture and 3/12 in explant culture. The AFP-producing capahity of these epithelial-like cells has been maintained from one week to one month in culture. 3. Cells derived from two TAE-nontreated cancer tissues were subculturable. One was established as a cholangiocellular carcinoma cell line. 4. No heterotransplantability of primary cultured cancer cells into nude mice was found. en-copyright= kn-copyright= en-aut-name=KusakaYasunori en-aut-sei=Kusaka en-aut-mei=Yasunori kn-aut-name=日下泰徳 kn-aut-sei=日下 kn-aut-mei=泰徳 aut-affil-num=1 ORCID= en-aut-name=TokiwaTakayoshi en-aut-sei=Tokiwa en-aut-mei=Takayoshi kn-aut-name=常盤孝義 kn-aut-sei=常盤 kn-aut-mei=孝義 aut-affil-num=2 ORCID= en-aut-name=SatoJiro en-aut-sei=Sato en-aut-mei=Jiro kn-aut-name=佐藤二郎 kn-aut-sei=佐藤 kn-aut-mei=二郎 aut-affil-num=3 ORCID= en-aut-name=TsugeHiroshi en-aut-sei=Tsuge en-aut-mei=Hiroshi kn-aut-name=津下宏 kn-aut-sei=津下 kn-aut-mei=宏 aut-affil-num=4 ORCID= en-aut-name=HamasakiKeisuke en-aut-sei=Hamasaki en-aut-mei=Keisuke kn-aut-name=浜崎啓介 kn-aut-sei=浜崎 kn-aut-mei=啓介 aut-affil-num=5 ORCID= en-aut-name=MimuraHisashi en-aut-sei=Mimura en-aut-mei=Hisashi kn-aut-name=三村久 kn-aut-sei=三村 kn-aut-mei=久 aut-affil-num=6 ORCID= en-aut-name=OritaKunzo en-aut-sei=Orita en-aut-mei=Kunzo kn-aut-name=折田薫三 kn-aut-sei=折田 kn-aut-mei=薫三 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設病理部門 affil-num=2 en-affil= kn-affil=岡山大学医学部癌源研究施設病理部門 affil-num=3 en-affil= kn-affil=岡山大学医学部癌源研究施設病理部門 affil-num=4 en-affil= kn-affil=岡山大学医学部第一外科学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第一外科学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第一外科学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部第一外科学教室 en-keyword=TAE kn-keyword=TAE en-keyword=ヒト肝癌 kn-keyword=ヒト肝癌 en-keyword=初代培養 kn-keyword=初代培養 en-keyword=株化 kn-keyword=株化 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=1-2 article-no= start-page=97 end-page=105 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Immunohistochemical studies on the binding of lectin (PNA) and human anti-liver cell membrane antibody to the hepatic carcinoma cell membrane of rats fed 3'-methyl-4-dimethylaminoazobenzene (DAB) kn-title=ラットDAB肝癌の免疫組織学的研究―レクチンおよびヒト由来の肝細胞膜抗体の反応性について― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Membrane changes of DAB hepatic carcinoma cells were examined immunohistochemically, using the binding activity of Arachis hypogaea agglutinin (PNA) and anti-liver membrane antibody (anti-LM) from patients with HBsAg-negative chronic active hepatitis. The hepatic carcinoma in rats was produced through DAB feedings for 3-5 months. As controls, non-cancerous liver tissues from rats fed DAB, and normal rat liver were used. In isolated calls of the hepatic carcinoma and control tissues, FITC-PNA binded in 10 of 11 cases of DAB hepatic carcinoma and in none of control cases (11 rats fed DAB and 2 normal rats). In tissue sections, similar results were obtained. Lens culnaris agglutinin did not show any difference in binding among the 3 groups. Two anti-LMs did not bind to the membranes of isolated calls in 3 of 10 DAB hepatic carcinomas, although the anti-LMs were bound in all of controls. These facts indicate that the transformation, DAB hepatic carcinoma cells is accompanied by changes in the cell membrane, and that PNA may be useful in the analysis of membrane changes in carcinogenesis. en-copyright= kn-copyright= en-aut-name=TomidaOsamu en-aut-sei=Tomida en-aut-mei=Osamu kn-aut-name=冨田治 kn-aut-sei=冨田 kn-aut-mei=治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科学教室 en-keyword=DAB肝癌 kn-keyword=DAB肝癌 en-keyword=PNA kn-keyword=PNA en-keyword=肝細胞膜抗体免疫蛍光法 kn-keyword=肝細胞膜抗体免疫蛍光法 en-keyword=レクチン蛍光法 kn-keyword=レクチン蛍光法 END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=11-12 article-no= start-page=1287 end-page=1297 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Chemosensitivity test using subrenal capsule assays Part 1. Experimental evaluation kn-title=Subrenal capsule assay法による制癌剤感受性試験に関する研究 第1編 Subrenal capsule assay法の基礎的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Experimental evaluation of a new chemosensitivity test using a subrenal capsule assays (SRC) was performed. The effects of various immunosuppressants-cyclosporin A (CSA), cyclophosphamide, whole body irradiation, and Bredinin were studied using human small cell lung cancer cell line (SBC-3) serially transplanted in nude mice. A significant degree of host cell infiltration was seen in tumor fragments implanted under the renal capsule of immunocompetent mice. However, treatment with immunosuppressants effectively suppressed the host immune reaction. The most effective immunosuppressant was CSA at 60mg/kg. We compared the antitumor activities of CDDP, MMC, VP-16, ADM, CPA, and VCR against SBC-3 using SRC and clonogenic assays. SRC was performed using mice administered CSA 60mg/kg. Chemotherapeutic agents (1/2 LD(10)) were administered on day 1 and antitumor activities were evaluated on day 6 after implantation. The results of the assays were well-correlated except with VCR. en-copyright= kn-copyright= en-aut-name=TadaAtsuhiko en-aut-sei=Tada en-aut-mei=Atsuhiko kn-aut-name=多田敦彦 kn-aut-sei=多田 kn-aut-mei=敦彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Subrenal capsule assay法 kn-keyword=Subrenal capsule assay法 en-keyword=制癌剤感受性試験 kn-keyword=制癌剤感受性試験 en-keyword=ヒト肺小細胞癌株 kn-keyword=ヒト肺小細胞癌株 END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=11-12 article-no= start-page=1267 end-page=1273 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A human monoclonal antibody against Pseudomonas aeruginosa and the possibility of its clinical use kn-title=緑膿菌に対するヒト型モノクローナル抗体の作成とその臨床応用の可能性 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A human monoclonal antibody (IgG) against Pseudomonas aeruginosa was established within 3 weeks after fusion of patient lymphocytes and human myeloma WIL2-86 cells. This method is useful in establishing human monoclonal antibodies for infectious bacteria of a variety of antigenicities and allows for use of such antibodies as immunotherapeutic drugs in chronically infected patients. The opsonic effect of the monoclonal antibody we established was 1.8-fold higher than that of commercially available immunoglobulin drugs. Since the established human monoclonal antibody recognizes serotype M, which has not yet been produced, the antibody may be used as a part of immunotherapeutic drug “cocktail”. en-copyright= kn-copyright= en-aut-name=KobayashiYouji en-aut-sei=Kobayashi en-aut-mei=Youji kn-aut-name=小林洋二 kn-aut-sei=小林 kn-aut-mei=洋二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部麻酔・蘇生学教室 en-keyword=ヒト型モノクローナル抗体 kn-keyword=ヒト型モノクローナル抗体 en-keyword=緑膿菌 kn-keyword=緑膿菌 en-keyword=M型O抗原 kn-keyword=M型O抗原 en-keyword=オプソニン kn-keyword=オプソニン END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=9-10 article-no= start-page=1119 end-page=1126 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the reactivity of human basophilic leukocytes Part 1. Correlation between morphological changes of human basophilic leukocytes and histamine release after stimulation with antigen or anti-IgE kn-title=好塩基球の反応性に関する研究 第1編 抗原，抗ヒトIgE刺激時の好塩基球の経時的形態的変化とヒスタミン遊離 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Morphological changes in human basophils from atopic asthmatics were observed in whole blood smears with respect to histamine release after stimulation with antigen or anti-IgE. Histamine was rapidly released and was accompanied by morphological changes in basophils upon stimulation with antigen and anti-IgE. A decreased number of basophils and morphological changes including increased cell diameters, increased ratios of the short to long axis diameters, and decreased intracellular granule counts were observed upon the release of histamine after antigen and anti-IgE stimulation. Antigen stimulus induced both more rapid histamine release and morphological changes than anti-IgE stimulation. en-copyright= kn-copyright= en-aut-name=NakagawaSaburo en-aut-sei=Nakagawa en-aut-mei=Saburo kn-aut-name=中川三郎 kn-aut-sei=中川 kn-aut-mei=三郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=気管支喘息 kn-keyword=気管支喘息 en-keyword=好塩基球 kn-keyword=好塩基球 en-keyword=形態的変化 kn-keyword=形態的変化 en-keyword=特異抗原 kn-keyword=特異抗原 en-keyword=抗ヒトIgE kn-keyword=抗ヒトIgE END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=9-10 article-no= start-page=1061 end-page=1074 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An immunohistochemical study of the localization of α and β subunits of S-100 protein in normal human brains and brain tumors kn-title=ヒト正常脳および脳腫瘍におけるS-100蛋白サブユニット(α鎖，β鎖)の局在に関する免疫組織化学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Immunohistochemical localization of α and β subunits of the S-100 protein in normal human brains and brain tumors was studied by the ABC method using mouse monoclonal antibodies against each subunit. In cerebral tissues, glial cells showed positive staining for both subunits, with some neurons positive only for the α subunit. In cerebellum, Purkinje cells and other neurons were negative for the α or β subunit, and Bergmann's cells were positive only for the β subunit. Low grade astrocytomas were strongly positive for the α and β subunits. In oligodendrogliomas and choroid plexus papillomas, the immunoreactivity of the β subunit was stronger than that of the α subunit. Medulloblastomas showed no positive staining for either subunit. Schwannomas were positive only for the β subunit. High grade astrocytomas, ependymomas and meningiomas manifested marked variability in the immunoreactivity for the α and β subunits of the S-100 protein. The evidence suggests that immunohistochemical examination of the α and β subunits of the S-100 protein in normal human brains and brain tumors may be useful in determining the histogenetic origin of brain tumors. en-copyright= kn-copyright= en-aut-name=TamiyaTakashi en-aut-sei=Tamiya en-aut-mei=Takashi kn-aut-name=田宮隆 kn-aut-sei=田宮 kn-aut-mei=隆 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳神経外科学教室 en-keyword=S-100蛋白α鎖サブユニット kn-keyword=S-100蛋白α鎖サブユニット en-keyword=S-100蛋白β鎖サブユニット kn-keyword=S-100蛋白β鎖サブユニット en-keyword=ヒト正常脳 kn-keyword=ヒト正常脳 en-keyword=ヒト脳腫瘍 kn-keyword=ヒト脳腫瘍 en-keyword=免疫組織化学 kn-keyword=免疫組織化学 END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=5-6 article-no= start-page=593 end-page=601 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Establishment of a new cell line derived from hepatocellular carcinoma exhibiting non-epithelial morphology kn-title=非上皮細胞様形態を呈するヒト肝細胞癌由来細胞株の樹立 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A cell line, HuH-33, was established in vitro from a patient with hepatocellular carcinoma (HCC), who had been treated with some chemotherapeutic agents. The chromosome number was widely distributed in 6888. This cell line has grown slowly, exhibiting a doubling time of approximately 150h, was intransplantable into nude mice, and secreted alpha-fetoprotein, albumin, β2-microglobulin, ferritin, tissue peptide antigen and extracellular matrix materials. HuH-33 was polygonal or spindle shaped, but it was keratin-positive and desmosome-like structures were observed with transmission electron microscopy, suggesting an epithelial origin of HuH-33. en-copyright= kn-copyright= en-aut-name=EndoAkira en-aut-sei=Endo en-aut-mei=Akira kn-aut-name=遠藤彰 kn-aut-sei=遠藤 kn-aut-mei=彰 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設病理部門 en-keyword=Hepatoma cell line kn-keyword=Hepatoma cell line en-keyword=cytokeratin kn-keyword=cytokeratin en-keyword=non-epithelial kn-keyword=non-epithelial END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=3-4 article-no= start-page=357 end-page=364 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An immunohistochemical and clinical study of human growth hormone in lung cancer kn-title=肺癌組織内ヒト成長ホルモンの免疫組織学的および臨床的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The human growth hormone (hGH) in lung cancer was studied immunohistochemically in 160 patients by the PAP method. Thirty-four neoplasm specimens (21.3%) contained hGH-positive tumor cells. Positive reaction for hGH was shown in 19 of 64 squamous cell carcinomas, 13 of 48 adenocarcinomas, 2 of 5 carcinods, and none of 26 small cell carcinomas and 17 large cell carcinomas. The sensitivity for hilar type lung cancer was higher than that for peripheral type (p<0.05). Positive rates for hGH were not affected by age, sex, tumor size, lymph node involvement or postsurgical stage. The survival rate of hGH positive patients was not worse than that of hGH negative. en-copyright= kn-copyright= en-aut-name=KunikataEiji en-aut-sei=Kunikata en-aut-mei=Eiji kn-aut-name=國方永治 kn-aut-sei=國方 kn-aut-mei=永治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二外科学教室 en-keyword=肺癌 kn-keyword=肺癌 en-keyword=ヒト成長ホルモン kn-keyword=ヒト成長ホルモン en-keyword=酵素抗体法 kn-keyword=酵素抗体法 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=医療用組換えヒトエリスロポエチンの品質および生物学的活性の管理 kn-title=Quality control and biological potency management of therapeutic recombinant human erythropoietin en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YanagiharaShigehiro en-aut-sei=Yanagihara en-aut-mei=Shigehiro kn-aut-name=柳原繁弘 kn-aut-sei=柳原 kn-aut-mei=繁弘 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト口腔細菌叢の健康時と病的時の変移に関する研究 kn-title=Analysis of Human Oral Bacterial Communities in Health and Disease en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WaelAmgad Refaat Ahmed Hassan en-aut-sei=Wael en-aut-mei=Amgad Refaat Ahmed Hassan kn-aut-name=ワエルアムカドレファット アハマド ハッサン kn-aut-sei=ワエル kn-aut-mei=アムカドレファット アハマド ハッサン aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=最終糖化産物はヒト混合リンパ球反応において単球機能の活性化を増強する kn-title=Advanced glycation end products enhance monocyte activation during human mixed lymphocyte reaction en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OhashiKatsuhisa en-aut-sei=Ohashi en-aut-mei=Katsuhisa kn-aut-name=大橋勝久 kn-aut-sei=大橋 kn-aut-mei=勝久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト血中生存浮遊がん細胞の簡易イメージングシステム kn-title=A simple biological imaging system for detecting viable human circulating tumor cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KojimaToru en-aut-sei=Kojima en-aut-mei=Toru kn-aut-name=児島亨 kn-aut-sei=児島 kn-aut-mei=亨 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue= article-no= start-page=29 end-page=33 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19830325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=好塩基球からのヒスタミン遊離に関する研究. 2. ハウスダスト抗原および抗ヒトIgEによるヒスタミン遊離 kn-title=Studies on the release of histamine from basophils 2. Histamine release induced by house dust extract and anti-IgE en-subtitle= kn-subtitle= en-abstract=好塩基球からのIgE-mediated histamine releaseの機序を,健康人(2名)および気管支喘息(4例)それぞれの代表例で比較検討した. 抗ヒトIgEの添加濃度別検討では,健康人および外因性喘息例いずれも有意のヒスタミン遊離の増加をしめしたが,Max. % histamine releaseをひきおこす抗ヒトIgEの濃度は比較的限られた範囲内にある傾向がみられた. 一方内因性喘息例では,いずれの抗ヒトIgE濃度でも有意のヒスタミン遊離はみられなかった. ハウスダスト抗原の添加濃度別検討では,ハウスダストが抗原である気管支喘息症例においてdose-dependentなヒスタミン遊離が観察されたが,使用された抗原濃度の範囲ではMax. % releaseをひきおこす至適濃度は明らかでなかった. kn-abstract=IgE-mediated release of histamine from whole blood was examined in two healthy and four asthmatic subjects by dose-dependent fashion. The significantly increased amount of histamine was released from basophils of both healthy and asthmatic subjects by a limited concentration of anti-IgE. Antigen (house dust) caused histamine release only from basophils of asthmatics who are sensitive to house dust. Basophils from one patients with asthma released no significant amount of histamine by anti-IgE. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=MifuneMasaaki en-aut-sei=Mifune en-aut-mei=Masaaki kn-aut-name=御般政明 kn-aut-sei=御般 kn-aut-mei=政明 aut-affil-num=4 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=5 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name=貴谷光 kn-aut-sei=貴谷 kn-aut-mei=光 aut-affil-num=6 ORCID= en-aut-name=GodaYoshinori en-aut-sei=Goda en-aut-mei=Yoshinori kn-aut-name=合田吉徳 kn-aut-sei=合田 kn-aut-mei=吉徳 aut-affil-num=7 ORCID= en-aut-name=TadaShinya en-aut-sei=Tada en-aut-mei=Shinya kn-aut-name=多田慎也 kn-aut-sei=多田 kn-aut-mei=慎也 aut-affil-num=8 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=6 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=7 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=8 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=9 en-affil= kn-affil=岡山大学医学部附属病院第2内科 END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue= article-no= start-page=23 end-page=28 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19830325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=好塩基球からのヒスタミン遊離に関する研究. 1 自動分析装置による全血からのヒスタミン遊離の測定 kn-title=Studies on the release of histamine from basophils 1. Determination of histamine from whole blood by an automated fluorometric histamine analysis system en-subtitle= kn-subtitle= en-abstract=ヒスタミン自動分析装置により,健康人10名,気管支喘息23例の全血からのヒスタミン遊離を測定した. 抗ヒトIgEを添加した際のヒスタミン遊離は,健康人および外因性気管支喘息症例では有意の増加傾向を示したが,一方内因性喘息症例では遊離増加はほとんどみられなかった. ハウスダスト抗原添加では,ハウスダストが抗原である気管支喘息症例においてのみ全血からの有意のヒスタミン遊離の増加が観察された. 以上の結果より,ヒスタミン自動分析装置による全血からの遊離ヒスタミンの測定は,気管支喘息の病態解明の1手段として極めて有用であると考えられる. kn-abstract=Histamine released from whole blood was determined by an automated fiuorometric histamine analysis system. The increased release of histamine from basophils by anti-IgE was observed in ten healthy subjects and 12 extrinsic asthma patients, while the release in 11 intrinsic asthma patients was significantly less as compared to that in healthy and extrinsic asthma subjects. House dust extract caused a significant increase in the histamine release from basophils of the extrinsic asthma patients who are sensitive to house dust. It was concluded from this study that histamine released from basophils could be easily determined by an automated analysis system and that the method is useful for the diagnosis and study of allergy. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=MifuneMasaaki en-aut-sei=Mifune en-aut-mei=Masaaki kn-aut-name=御般政明 kn-aut-sei=御般 kn-aut-mei=政明 aut-affil-num=4 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=5 ORCID= en-aut-name=OhtaniJun en-aut-sei=Ohtani en-aut-mei=Jun kn-aut-name=大谷純 kn-aut-sei=大谷 kn-aut-mei=純 aut-affil-num=6 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name=貴谷光 kn-aut-sei=貴谷 kn-aut-mei=光 aut-affil-num=7 ORCID= en-aut-name=GodaYoshinori en-aut-sei=Goda en-aut-mei=Yoshinori kn-aut-name=合田吉徳 kn-aut-sei=合田 kn-aut-mei=吉徳 aut-affil-num=8 ORCID= en-aut-name=TadaShinya en-aut-sei=Tada en-aut-mei=Shinya kn-aut-name=多田慎也 kn-aut-sei=多田 kn-aut-mei=慎也 aut-affil-num=9 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=6 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=7 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=8 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=9 en-affil= kn-affil=岡山大学医学部附属病院第2内科 affil-num=10 en-affil= kn-affil=岡山大学医学部附属病院第2内科 END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue= article-no= start-page=35 end-page=38 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19840325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=好塩基球からのヒスタミン遊離に関する研究. 3. 抗ヒトIgE に対する好塩基球の反応性と末梢血好酸球増多 kn-title=Studies on the release of histamine from basophils. 3. Correlation between basophil reactivity to anti-IgE and blood eosinophilia en-subtitle= kn-subtitle= en-abstract=末梢血好酸球増多を示す気管支喘息14症例について,抗ヒトIgE による好塩基球からのヒスタミン遊離を検討した. ハウスダストに対する皮内反応およびRASTが陽性を示す症例群(groupI)では,血清IgE値が高く,また抗ヒトIgEによるMax. % ヒスタミン遊離も高く(50.7士5.0%),そのdoseresponse curveは抗ヒトIgE添加濃度が高くなるにつれて上昇する傾向を示した.一方種々のアレルゲンエキスに対する皮内反応が陰性で,かつRAST も陰性を示す症例群(group II)では,血清IgE値が低く,また抗ヒトIgEによるMax. % ヒスタミン遊離も低く(10.5士1.7%),そのdose-response curveは全般的に低く,抗ヒトIgE添加濃度が高いところではむしろ抑制される傾向を示した. kn-abstract=Histamine release from basophils induced by anti-IgE was examined in 14 asthmatic subjects with blood eosinophilia. The subjects were divided into two groups; group I (with blood eosinophilia, positive skin test and positive RAST to house dust) and group II (with blood eosinophilia, negative skin test to various allergens and negative RAST to house dust). 1. Serum IgE levels in group I were much higher than those in group II. 2. Maximum percent histamine release induced by anti-IgE was much higher in group I than in group II. 3. Dose-response curve of anti-IgE-induced histamine release in group I showed consistent increase as anti-IgE concentrations increased. while, dose-response curve in group II was very low. These findings suggested that blood eosinophilia in group I might be elicited relating to IgE-mediated reaction. However, mechanism causing eosinophilia in group II was not clear in the present study. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=MifuneMasaaki en-aut-sei=Mifune en-aut-mei=Masaaki kn-aut-name=御般政明 kn-aut-sei=御般 kn-aut-mei=政明 aut-affil-num=4 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=5 ORCID= en-aut-name=NakagawaSaburo en-aut-sei=Nakagawa en-aut-mei=Saburo kn-aut-name=中川三郎 kn-aut-sei=中川 kn-aut-mei=三郎 aut-affil-num=6 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name=貴谷光 kn-aut-sei=貴谷 kn-aut-mei=光 aut-affil-num=7 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=6 en-affil= kn-affil=岡山大学医学部第2内科 affil-num=7 en-affil= kn-affil=岡山大学医学部第2内科 affil-num=8 en-affil= kn-affil=岡山大学医学部第2内科 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue= article-no= start-page=7 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19841130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Spa therapy for patients with chronic obstructive lung disease kn-title=Studies on the release of histamine from basophils 4. Difference between house dust- and Candida-induced secretion en-subtitle= kn-subtitle= en-abstract=ハウスダストあるいはカンジダが特異抗原である気管支喘息30例を対象に,それぞれの抗原刺激時の好塩基球からのヒスタミン遊離を,抗ヒトIgEによる遊離と比較検討した. 1. ハウスダストが原因抗原である症例の好塩基球からのヒスタミン遊離は,抗原刺激時41.8%,抗ヒトIgE刺激時40.8%であった. 2. カンジダが原因抗原である症例の好塩基球からのヒスタミン遊離は,抗原刺激時27.1%,抗ヒトIgE刺激時32.2%であった. 3. ハウスダストと抗ヒトIgEによる好塩基球からのヒスタミン遊離は,ほぼ同様の値を示した. 一方カンジダと抗ヒトIgEによるヒスタミン遊離の間には有意の 相関関係はみられなかった. kn-abstract=Histamine releasse from basophils induced by house dust and C. albicans was examined in 30 patients with bronchial asthma. House dust and C. albicans caused a significant amount of histamine release in subjects with a RAST score to corresponding allergen. A close correlation was found between house dust- and anti-IgE-induced histamine release. However, histamine release induced by C. albicans was considerably different from the release induced by anti-IgE. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=4 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name=貴谷光 kn-aut-sei=貴谷 kn-aut-mei=光 aut-affil-num=5 ORCID= en-aut-name=NakagawaSaburo en-aut-sei=Nakagawa en-aut-mei=Saburo kn-aut-name=中川三郎 kn-aut-sei=中川 kn-aut-mei=三郎 aut-affil-num=6 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学第2内科 affil-num=6 en-affil= kn-affil=岡山大学第2内科 affil-num=7 en-affil= kn-affil=岡山大学第2内科 END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue= article-no= start-page=17 end-page=21 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=好塩基球からのヒスタミン遊離に関する研究. 5.臨床的評価 kn-title=Studies on the release of histamine from basophils 5. Clinical evaluation en-subtitle= kn-subtitle= en-abstract= kn-abstract=過去3年間にわたり,気管支喘息患者末梢血好塩基球からのヒスタミン遊離について,ヒスタミン自動分析装置を用いて全血法により検討を加えてきた. 1. 抗ヒトIgE によるヒスタミン遊離は,症例間で著しい差がみられた. この際血清IgE値が高値を示す症例では全般的に高度なヒスタシソ遊離がみられたが,血清IgE値が正常かまたはむしろ低値を示す症例のヒスタミン遊離は,かなり高度なものから全くみられないものまでさまざまであった. 2.ハウスダストやカンジダなどの抗原物質によるヒスタミン遊離は,特異的IgE抗体依存性であり,抗体価が上昇するにつれヒスタミン遊離は高度となる傾向がみられた. しかし,ハウスダストとカンジダによるヒスタミン遊離には若干の差がみられた. すなわち,ハウスダストと抗ヒトIgE によるヒスタミン遊離の間には密接な関連がみられたが,カンジダと抗ヒトIgEの間には全く関連がみられなかった. またカンジダによるヒスタミン遊離においては,IgE系反応以外の反応が関与する可能性が一部示唆された. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学三朝分院内科 END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue= article-no= start-page=2 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=環境の発ガン物質に対するヒト曝露の研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HayatsuHikoya en-aut-sei=Hayatsu en-aut-mei=Hikoya kn-aut-name=早津彦哉 kn-aut-sei=早津 kn-aut-mei=彦哉 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学薬学部 END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue= article-no= start-page=12 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199809 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=癌の遺伝子治療 en-subtitle= kn-subtitle= en-abstract= kn-abstract=癌抑制遺伝子p53の突然変異や欠損による機能的異常は､多くのヒト癌で普遍的かつ高頻度に認められている｡p53タンパク質の機能の一つとしては､細胞増殖に関するいろいろな遺伝子の発現制御を介した細胞周期の調節が考えられているが､その他に最近アポトーシスの誘導分子としても注目を浴びてきている｡正常型p53遺伝子を有する胃癌､大腸癌では､術前化学療法や放射線療法で癌細胞のアポトーシスが誘導されたが､変異型p53を発現する腫瘍 ではアポトーシスに陥った細胞はほとんど認められなかった｡ヌードマウスの皮下に移植したp53遺伝子に異常を持つヒト肺癌腫瘍にリコンビナント･アデノウイルスベクターを用いて正常型p53遺伝子を導入すると､抗癌剤に対する感受性が劇的に増強し､シスプラチンの腹腔内投与により腫瘍内にアポトーシスによる広範囲な組織破壊が認められた｡この正常型p53発現アデノウイルスベクターとDNA障害性抗癌剤を併用した遺伝子治療は､臨床的にヒト悪性腫癌に応用可能と考えられる｡ en-copyright= kn-copyright= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name=田中紀章 kn-aut-sei=田中 kn-aut-mei=紀章 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科 END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue= article-no= start-page=35 end-page=37 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=200409 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of High-Fat and High-Calorie Diets on the Onset of Diabetes in Meg1/Grb10 Transgenic Mice kn-title=高脂肪･高カロリー飼料がMeg1/Grb10遺伝子導入マウスの糖尿病発症に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Meg1/Grb10遺伝子導入マウスはインスリンのシグナル伝達阻害による高インスリン血症を呈することから､II型糖尿病モデルと考えられている｡そこで､本モデルマウスを用いてII型糖尿病発症に及ぼす飼料の影響について検討した結果､ヒトII型糖尿病モデルとして有用性を確認することが出来た｡ en-copyright= kn-copyright= en-aut-name=YamamotoYoshie en-aut-sei=Yamamoto en-aut-mei=Yoshie kn-aut-name=山本美江 kn-aut-sei=山本 kn-aut-mei=美江 aut-affil-num=1 ORCID= en-aut-name=SuzukiOsamu en-aut-sei=Suzuki en-aut-mei=Osamu kn-aut-name=鈴木治 kn-aut-sei=鈴木 kn-aut-mei=治 aut-affil-num=2 ORCID= en-aut-name=Yamada-UchioKozue en-aut-sei=Yamada-Uchio en-aut-mei=Kozue kn-aut-name=山田‐内尾こずえ kn-aut-sei=山田‐内尾 kn-aut-mei=こずえ aut-affil-num=3 ORCID= en-aut-name=Ishino-KanekoTomoko en-aut-sei=Ishino-Kaneko en-aut-mei=Tomoko kn-aut-name=石野‐金児智子 kn-aut-sei=石野‐金児 kn-aut-mei=智子 aut-affil-num=4 ORCID= en-aut-name=MatsudaJunichirou en-aut-sei=Matsuda en-aut-mei=Junichirou kn-aut-name=松田潤一郎 kn-aut-sei=松田 kn-aut-mei=潤一郎 aut-affil-num=5 ORCID= en-aut-name=SatoKatsunori en-aut-sei=Sato en-aut-mei=Katsunori kn-aut-name=佐藤勝紀 kn-aut-sei=佐藤 kn-aut-mei=勝紀 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院自然科学研究科 affil-num=2 en-affil= kn-affil=国立感染症研究所獣医科学部 affil-num=3 en-affil= kn-affil=国立感染症研究所獣医科学部 affil-num=4 en-affil= kn-affil=東海大学健康科学部 affil-num=5 en-affil= kn-affil=国立感染症研究所獣医科学部 affil-num=6 en-affil= kn-affil=岡山大学農学部 END start-ver=1.4 cd-journal=joma no-vol=24 cd-vols= no-issue= article-no= start-page=19 end-page=21 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200712 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study on the usefulness of Meg1Crb10 transgenic mouse as aType2 diabetes mellitus model animal. -Analysis of blood plasma component and expression of genes related to onset of diabetes - kn-title=2型糖尿病モデル動物Meg1/Grb10遺伝子導入マウスの有用性の検討 - 血漿成分及び糖尿病関連遺伝子の発現と発症の解析 - en-subtitle= kn-subtitle= en-abstract= kn-abstract=Meg1/Grb10遺伝子導入マウス（Meg1マウス）はインスリンのシグナル伝達阻害による高インスリン血症を呈することから2型糖尿病モデルと考えられている。Meg1マウスは肥満を伴なわずに高血糖を発症するが､脂肪･カロリーの過剰摂取によっても糖尿病の発症が著しく増加する｡本研究はMeg1マウスの2型糖尿病モデルとしての有用性を検討するために､Meg1マウスと対照マウスを高脂肪･高カロリー飼料（HFD）及び対照飼料（NFD）で飼育した時の血漿アディポネクチン量とBMI値を比較するとともに糖尿病関連遺伝子の発現量について他の糖尿病モデルマウスと比較検討した。血漿アディポネクチン量はMeg1マウスのHFDが最も高く､対照マウスのNFDが最も低い値を示した。一方､BMI値は対照マウスのHFDが最も高い値を示し､血漿アディポネクチン量とBMI値は逆相関が認められ､ヒト2型糖尿病と類似することが認められた。また､Grb10､Glut4遺伝子の発現量はMeg1マウスと他の糖尿病モデルマウスでは異なる値を示し､Meg1マウスでのGrb10遺伝子の発現量は高く､Glut4遺伝子の発現量は低かった。以上のことから､Meg1マウスには他の糖尿病モデルと異なる発症機構の存在が示唆され､Meg1マウスは2型糖尿病モデルとしての有用性が考えられた。 en-copyright= kn-copyright= en-aut-name=YamamotoYoshie en-aut-sei=Yamamoto en-aut-mei=Yoshie kn-aut-name=山本美江 kn-aut-sei=山本 kn-aut-mei=美江 aut-affil-num=1 ORCID= en-aut-name=SuzukiOsamu en-aut-sei=Suzuki en-aut-mei=Osamu kn-aut-name=鈴木治 kn-aut-sei=鈴木 kn-aut-mei=治 aut-affil-num=2 ORCID= en-aut-name=Yamada-UchioKozue en-aut-sei=Yamada-Uchio en-aut-mei=Kozue kn-aut-name=山田-内尾こずえ kn-aut-sei=山田-内尾 kn-aut-mei=こずえ aut-affil-num=3 ORCID= en-aut-name=Ishino-KanekoTomoko en-aut-sei=Ishino-Kaneko en-aut-mei=Tomoko kn-aut-name=石野-金児智子 kn-aut-sei=石野-金児 kn-aut-mei=智子 aut-affil-num=4 ORCID= en-aut-name=MatsudaJunichirou en-aut-sei=Matsuda en-aut-mei=Junichirou kn-aut-name=松田潤一郎 kn-aut-sei=松田 kn-aut-mei=潤一郎 aut-affil-num=5 ORCID= en-aut-name=SatoKatsunori en-aut-sei=Sato en-aut-mei=Katsunori kn-aut-name=佐藤勝紀 kn-aut-sei=佐藤 kn-aut-mei=勝紀 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院自然科学研究科 affil-num=2 en-affil= kn-affil=医薬基盤研究所生物資源研究部 affil-num=3 en-affil= kn-affil=医薬基盤研究所生物資源研究部 affil-num=4 en-affil= kn-affil=東海大学健康科学部 affil-num=5 en-affil= kn-affil=医薬基盤研究所生物資源研究部 affil-num=6 en-affil= kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=9 article-no= start-page=1305 end-page=1308 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=19560930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Parathion-Splitting Enzyme. Part 2. Parathion-splitting activity of human blood serum and distribution of parathion-splitting enzyme in various organs of dogs kn-title=パラチオン分解酵素に関する研究 U. ヒト血清のパラチオン分解能及びパラチオン分解酵素の諸臓器（イヌ）内分布に就て en-subtitle= kn-subtitle= en-abstract= kn-abstract=Human blood serum and various organs of dogs were determined for its content of parathion-splitting enzyme by the procedure presented in the preceding paper. Ten per cent saline suspension of the relevant homogenized tissues was used for the estimation of parathion-splitting enzyme of the organs. (1) The activity of the enzyme in blood serum of normal individuals was 134 ± 44.6 γ/ml for men and 180 ± 62 γ/ml for women. (2) Of the various organs of dogs the blood serum was the most abundant in the enzyme, and the liver, the lung, the bone marrow and the kidney followed in the order given. The distribution of the enzyme was not appreciable in other organs. (3) The blood, the liver, the lung and the bone marrow were thought to play a major part in the detoxication of parathion, because the afore-mentioned decomposition of this poison to the less toxic paranitrophenol was believed to imply detoxication. The blood will constitute the most important and the liver the next-important organ of detoxication, if volume of the organs is taken into account. en-copyright= kn-copyright= en-aut-name=ShiigiTeiji en-aut-sei=Shiigi en-aut-mei=Teiji kn-aut-name=椎木悌二 kn-aut-sei=椎木 kn-aut-mei=悌二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=山口県立医科大学臨床病理学教室 END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue= article-no= start-page=31 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=気管支喘息および慢性気管支炎患者における抗ヒトIgEによる好塩基球からのヒスタミン遊離 kn-title=Basophil histamine release by anti-IgE in Subjects of chronic bronchitis and bronchial asthma en-subtitle= kn-subtitle= en-abstract=気管支喘息50例，慢性気管支炎8例を対象に，抗ヒトIgE添加時の好塩基球からのヒスタミン遊離を全血法により行ない，その臨床的評価について検討を加えた。抗ヒトIgE海底時のMax　％　histamine releaseの平均は，健康人24.7±14.2％，慢性気管支炎27.7±22.1％，気管支喘息28.4±17.0％であり，3者間に有意の差はみられなかった。すなわち，抗ヒトIgE添加により健康人や慢性気管支炎患者の好塩基球からも有意のヒスタミン遊離が見られた。気管支喘息のなかでは，内因性喘息症例においてヒスタミン遊離（14.1±7.2％）の低い傾向が見られた。Dose-response curveの検討では，健康人，慢性気管支炎症例では全例E（2）からE（1）へかけてのnegative slopeを示した。気管支喘息症例では，血清IgE値500IU/ml以下の症例ではnegative slopeを示す症例が多く，一方501IU/ml以上の症例ではpositive slopeを示す症例がより多く見られた。 kn-abstract=Histamine release from basophils induced by anti-IgE was studied in 8 patients with chronic bronchitis and 50 patients with bronchial asthma by analyzing doseresponse curves. As the result, there were no significant differences in maximum percent histamine release from basophils among three groups of healthy subjects (24.7± 14.2%), patients with chronic bronchitis (27.7±22.1%) and those with bronchial asthma (28.4±17.0%). In the patients with bronchial asthma, the maximum percent histamine release was higher in accordance with higher serum IgE levels, and low maximum percent release was observed in patients with intrinsic asthma (14.1±7.2%). Study of dose-response curves of anti-IgE-induced histamine release showed that a negative slope from E(2) to E(1) was observed in both healthy subjects and patients with chronic bronchitis. The majority of asthmatics with serum IgE levels of 501IU/ml or over showed a positive slope from E(2) to E(1). en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=2 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name=貴谷光 kn-aut-sei=貴谷 kn-aut-mei=光 aut-affil-num=3 ORCID= en-aut-name=ArakiHiroyuki en-aut-sei=Araki en-aut-mei=Hiroyuki kn-aut-name=荒木洋行 kn-aut-sei=荒木 kn-aut-mei=洋行 aut-affil-num=4 ORCID= en-aut-name=TsujiMitsuaki en-aut-sei=Tsuji en-aut-mei=Mitsuaki kn-aut-name=辻光朗 kn-aut-sei=辻 kn-aut-mei=光朗 aut-affil-num=5 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name=高橋清 kn-aut-sei=高橋 kn-aut-mei=清 aut-affil-num=6 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=6 en-affil= kn-affil=岡山大学医学部第2内科 affil-num=7 en-affil= kn-affil=岡山大学医学部第2内科 en-keyword=Basophil response (好塩基球の反応性) kn-keyword=Basophil response (好塩基球の反応性) en-keyword=Anti-lgE (抗ヒトIgE) kn-keyword=Anti-lgE (抗ヒトIgE) en-keyword=Histamine release (ヒスタミン遊離) kn-keyword=Histamine release (ヒスタミン遊離) en-keyword=chronic bronchitis (慢性気管支炎) kn-keyword=chronic bronchitis (慢性気管支炎) en-keyword=bronchial asthma (気管支喘息) kn-keyword=bronchial asthma (気管支喘息) END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue= article-no= start-page=5 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study on anti-allergic action of Terfenadine kn-title=Terfenadineの抗アレルギー作用について en-subtitle= kn-subtitle= en-abstract=Anti-allergic action of terfenadine, one of the H(1) receptor inhibitors, was examined by human basophil histamine release induced by anti-IgE or a specific allergen (house dust), and the release of leukotriens from leucocytes stimulated by Ca ionophore A23187. 1. Terfenadine did not inhibit the release of histamine from basophils of healthy subjects induced by anti-IgE, and also of patients with bronchial asthma induced by house dust when the in vitro examination was performed by a whole blood method. 2. Histamine release from basophils of patients with bronchial asthma was significantly inhibited when washed leucocytes was preincubated with terfenadine in vitro. The release of LT B(4) from leucocytes was also inhibited. 3. In vivo examination of inhibitory action of terfenadine showed no effect on basophil histamine release of healthy subjects by anti-IgE and of asthmatic subjects by house dust, although skin test by house dust in these subjects was inhibited 60 min after administration in vivo. These results show that terfenadine inhibits the release of chemical mediators from tissue mast cells, and basophils migrating into local allergic sites. kn-abstract=選択的H(1)受容体拮抗薬であるTerfenadineの抗アレルギー作用，特にIgE系反応によるヒスタミン，およびCa ionophore A23187による好中球からのleukotrienes遊離に対する抑制作用を，ヒト末梢血白血球を用いて検討した。１．Terfenadineは，抗ヒトIgE刺激による健康人好塩基球および特異抗原刺激による気管支喘息患者好塩基球からのヒスタミン遊離に対して，全血法でのin vitro添加実験では有意の抑制作用を示さなかった。 2．洗浄白血球法によるin vitro添加実験では，Terfenadineは特異抗原および抗ヒトIgE刺激時の気管支喘息患者好塩基球からのヒスタミン，Ca ionophore　A23187刺激時のleukotriene B(4)遊離に対して有意の抑制効果を示した。3．Terfenadineの内服によるin vivoの抑制実験では，IgE系反応による健康人および気管支喘息患者好塩基球からのヒスタミン遊離に対して有意の抑制効果は見られなかったが，特異抗原による皮膚反応に対しては明らかな抑制作用が観察された。以上より，Terfenadineは，組織肥満細胞および組織へと遊走してきた好塩基球に対してはある程度の抑制作用を有しているものと考えられた。 en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=KitaniHikaru en-aut-sei=Kitani en-aut-mei=Hikaru kn-aut-name=貴谷光 kn-aut-sei=貴谷 kn-aut-mei=光 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部三朝分院内科 en-keyword=夕一フェナンジン (Terfenadine) kn-keyword=夕一フェナンジン (Terfenadine) en-keyword=lgE系反応 (IgE-mediated reaction) kn-keyword=lgE系反応 (IgE-mediated reaction) en-keyword=ヒスタミン (histamine) kn-keyword=ヒスタミン (histamine) en-keyword=好塩基球 (basophils) kn-keyword=好塩基球 (basophils) END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue= article-no= start-page=42 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=198607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on anti-allergic actions of tranilast (Rizaben?) kn-title=Tranilast(Rizaben?)の抗アレルギー作用について en-subtitle= kn-subtitle= en-abstract=Tlanilast is clinically used for bronchial asthma as one of the prophylactic agents for asthma attacks. In this study, anti-allergic actions of tranilast were examined in (45)Ca uptake of and histamine release from target cells of IgE after stimulation with antigen, anti-IgE and camp. 48/80. 1. (45)Ca uptake of and histamine release from rat peritoneal mast cells stimulated by antigen were significantly inhibited by preincubation of the cells with tranilast. Inhibition by tranilast of increased (45)Ca uptake of mast cells by addition of phosphatidylserine were less, although inhibition of histamine release was not so affected by addition of phosphatidylserine. No significant inhibition by tranilast of (45)Ca uptake and histamine release was not observed when the cells were stimulated by camp. 48/80. 2. Tranilast showed any inhibitory effects on basophil histamine release induced by antigen and anti-IgE. kn-abstract=抗アレルギー剤（脱顆粒抑制剤）の1つであるtranilastの肥満細胞の遊離機序に対する抑制作用について検討を加えた。1．抗原刺激時の肥満細胞の(45)Ca uptakeおよびヒスタミン遊離に対して，tranilastは有意の抑制作用を示したが，comp.48/80刺激時にはtranilastの(45)Ca uptake，ヒスタミン遊離に対する抑制作用はほとんどみられなかった。2．phosphatidylserine添加時には，(45)Ca uptakeに対するtranilastの抑制作用は減弱傾向を示し，この傾向はヒスタミン遊離に対する作用に比べより高度であった。3．抗原および抗ヒトIgEによる好塩基球からのヒスタミン遊離に対して，tranilastは明らかな抑制作用を示さなかった。 en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=2 ORCID= en-aut-name=SudoMichiyasu en-aut-sei=Sudo en-aut-mei=Michiyasu kn-aut-name=周藤真康 kn-aut-sei=周藤 kn-aut-mei=真康 aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=4 ORCID= en-aut-name=OhtaniJun en-aut-sei=Ohtani en-aut-mei=Jun kn-aut-name=大谷純 kn-aut-sei=大谷 kn-aut-mei=純 aut-affil-num=5 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属環境病態研究施設基礎環境病態学分野 affil-num=2 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学医学部第2内科 affil-num=6 en-affil= kn-affil=岡山大学医学部第2内科 en-keyword=トラニラスト (tranilast) kn-keyword=トラニラスト (tranilast) en-keyword=(45)Ca取り込み ((45)Ca uptake) kn-keyword=(45)Ca取り込み ((45)Ca uptake) en-keyword=ヒスタミン遊離 (histamine release) kn-keyword=ヒスタミン遊離 (histamine release) en-keyword=肥満細胞 (mast cell) kn-keyword=肥満細胞 (mast cell) en-keyword=好塩基球 (basophils) kn-keyword=好塩基球 (basophils) END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=5-1 article-no= start-page=2293 end-page=2301 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590415 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Experimental Study of the Specific Protein Contained in Human Fetal Serum Globulin kn-title=ヒト胎児血清グロブリン中に含有する特異蛋白に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=1) When rabbits are immunized by human fetal serum, high titer precipitin reaction on human serum can be obtained by injecting ten times. 2) When rabbits are immunized by human fetal serum with Globulin and Albumin precipitin productions can be obtained easier than that of human fetal serum. As for each antigenity, the remarkable difference can not be seen and the antigenity of fetal serum Albumin is by no means inferior to that of Globulin. 3) In case of fetal serum Albumin as antigen, precipitin quantity is lower than that of Globulin, and appearance of reaction is less. 4) Specific protein contained in fetal serum, exists in serum Globulin, not in Albumin. en-copyright= kn-copyright= en-aut-name=MiyoshiYoshinori en-aut-sei=Miyoshi en-aut-mei=Yoshinori kn-aut-name=三好義則 kn-aut-sei=三好 kn-aut-mei=義則 aut-affil-num=1 ORCID= en-aut-name=OchiaiYoshiyuki en-aut-sei=Ochiai en-aut-mei=Yoshiyuki kn-aut-name=落合義幸 kn-aut-sei=落合 kn-aut-mei=義幸 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部法医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部法医学教室 END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=2-1 article-no= start-page=555 end-page=565 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sero-immunological Studies of the Specific Protein Contained in Human Meconium kn-title=ヒト胎便中に含有する特異蛋白の血清免疫学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Author intended the sero-immunologic studies of human meconium as the series of studies concerning fetal serum, placenta and so on, and then obtained the results as follows; 1) When rabbits are injected repeatedly with the extract of human meconium, the antiserum can be obtained high titer precipitin reaction on human serum. 2) Researching production of precipitin about rabbit's serum immunized with human meconium of the 7, 8, 9 and 10th pregnant months separatedly, author cannot find striking differences on antigenity in each months. 3) Rabbit's serum immunized with human meconium has very strong species specifity. 4) In human meconium exist specific protein fractions which are not contained in the adult human serum and these fractions are considered to coincide with those exist in the fetal serum and there were very small quantities. en-copyright= kn-copyright= en-aut-name=MiyoshiYoshinori en-aut-sei=Miyoshi en-aut-mei=Yoshinori kn-aut-name=三好義則 kn-aut-sei=三好 kn-aut-mei=義則 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部法医学教室 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=7 article-no= start-page=2415 end-page=2426 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=1958 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=On the Allergic Histological Changes that have occurred on the Organs of Pregnant Rabbits due to Injection of Human Placental Extract as well as Fetal Serum Protein kn-title=ヒト胎盤ならびに胎児血清蛋白による妊娠ウサギ臓器のアレルギー性組織変化について 第2編 ヒト胎児血清,胎盤ならびに羊水蛋白による妊娠ウサギ臓器のアレルギー性組織変化について en-subtitle= kn-subtitle= en-abstract=It has been discovered that there exists, within the fetal serum, as well as human placental extract and an amniotic fluid, the specific protein that hadn't found out in any human adult serum; which has the similar subjects to above. They were repeatedly injected to pregnant rabbits as antigens, and the following are the results. There are caused the allergic histological changes on liver and kidney outstandingly, the changes are alike to the organs of pregnant toxicosis, and are the most strongest when injected placental protein, more stonger fetal serum than amniotic fluid. However any differences cann't find out among themselves histologically, so the author has considered that the specific protein is caused to the above said changes. kn-abstract= en-copyright= kn-copyright= en-aut-name=OkamuraYasumasa en-aut-sei=Okamura en-aut-mei=Yasumasa kn-aut-name=岡村安政 kn-aut-sei=岡村 kn-aut-mei=安政 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=1 article-no= start-page=97 end-page=103 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=19580131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=On the Allergic Histologial Changes that have occurred due to Human Placenta as well as Fetus Serum Protein in Pregnant Rabbit's Organs kn-title=ヒト胎盤ならびに胎児血清蛋白による妊娠ウサギ臓器のアレルギー性組織変化について en-subtitle= kn-subtitle= en-abstract= kn-abstract=It has been discovered that there exists, within the fetus serum, as well as human placenta, a similar specific protein that one cannot find out in any human serum; which has been considered to have been formulated in placenta; further, one can probe out the abovesaid unique protein part in a show alike. en-copyright= kn-copyright= en-aut-name=OkamuraYasumasa en-aut-sei=Okamura en-aut-mei=Yasumasa kn-aut-name=岡村安政 kn-aut-sei=岡村 kn-aut-mei=安政 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部法医学教室 END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=5-7 article-no= start-page=1463 end-page=1469 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=19600730 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Influences of Electrical Stimulation (In Vitro) on the Metabolism of the Brain Part 3. Influences of Electrical Stimulation (in vitro) on the Cerebral Transamination kn-title=電気刺戟（in vitro）の脳物質代謝におよぼす影響 第3編 電気刺戟（in vitro）の大脳トランスアミネーションにおよぼす影響 en-subtitle= kn-subtitle= en-abstract=With the use of homogenate of the brains and cerebral cortex slices obtained from albino rats, Pithecus monkey and human, the author observed the influences of electrical stimulation (in vitro) on the fransamination, and obtained the following results. 1. In the case of albino rat cerebrum the electrical stimulation inhibited the synthesis of glutamic acid from the aspatic acid 14.6 per cent with homogenate and 9.6 per cent with slices. Namely, the rate of such inhibition seems to he greater in the case of homogenate than in the case of slices. In addition, in the alanine series ne marked changes could be recognired but in GABA series all tended to receive a slight inhibitory effect. In all cases, however, the rate of reactivity is diminished more with slices than with homogenates. 2. In the case of the cerebral cortex homogenate of monkey, the electrical stimulation inhibited the glutamic and production in the aspartic acid seriet about 11 per cent on the average. In the case of the normal human cerebral cortex slices, the electrical stimulation accelerated the glutamic acid production about 11.3 per cent on the average, while in the case of atrophic human cerebral cortex homogenate hardly any changes could be recognized. In comparing the rats of reactivity in the case of the normal human cerebral cortex slices it is about 20 per cent higher both in the control group and experimental group than in the case of the slices of albino rats. In the cisa of the atrophic human cerebral cortex homogenate the reactivity was slightly lower than in the case of albino rats. kn-abstract=1) ダイコクネズミ,タイワンザル,ヒト脳の大脳ホモジネートおよび大脳皮質切片を用いて電気刺戟(in vitro)のトランスアミネーションにおよぼす影響をみた. 2) ダイコクネズミ大脳においてはAsGT活性は電気刺戟によつてホモジネートでは14.6%切片では9.6%阻害された.すなわちホモジネートの方が切片より阻害率は著明なようであつた.またAIGT活性は電気刺戟によつてホモジネート,切片とも著変なく,γAGT活性は電気刺戟によつてホモジネート,切片とも阻害傾向を示した.そこでAIGTは酵素系が異なるためではないかと推論した.また反応率はいづれの場合も切片の方がホモジネートより減少していた. 3) タイワンザル大脳皮質ホモジネートにおいてはAsGT活性は電気刺戟によつて平均約11%阻害された.またヒト健常大脳皮質切片においてはAsGT活性は電気刺戟によつて平均約11.3%の促進をみ,ヒト萎縮大脳皮質ホモジネートにおいては殆ど変化をみなかつた.また反応率を比較するとヒト健常大脳皮質切片ではダイコクネズミの切片の場合に比し対照時,刺戟時ともに約20%高く,ヒト萎縮大脳皮質ホモジネートではダイコクネズミのそれに比しやや低い値を示した.すなわち萎縮脳のAsGT活性の低下が考えられる. en-copyright= kn-copyright= en-aut-name=KumashiroHisashi en-aut-sei=Kumashiro en-aut-mei=Hisashi kn-aut-name=熊代永 kn-aut-sei=熊代 kn-aut-mei=永 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=10 article-no= start-page=903 end-page=912 dt-received= dt-revised= dt-accepted= dt-pub-year=1963 dt-pub=19631030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Megakaryocytes in the Spleen by Splenic Tissue Culture Method Part 3. Megakaryocytes in the Human Spleen kn-title=脾組織培養法による脾内巨核球に関する研究 第3編 ヒト脾内巨核球について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Followings are the results of studies by our clinical tissue culture method mainly on motility and ability of platelet formation of megakaryocytes in the spleen of human embryo and a patient with osteomyelofibrosis. 1) Megakaryocytes are always found in the spleen of human embryo, and no significant morphological differences are noted from those in the bone marrow. 2) Deformated movement, pseudopodial movement, and tentacle formation for the platelet formation are noted on megakaryocytes in the spleen of human embryos. As for functions of megakaryocytes, motility and ability of the platelet formation are significantly found at 4 month of age, but are remarkably decreased after 5 month of age. 3) A correlation is noted in human embryo between the functions of megakaryocytes and erythropoiesis in the spleen, a rise and fall of the one being accompanied by the other. 4) In a patient with osteomyelofibrosis, megakaryocytes found in the spleen show a remarkable decrease in the functions. en-copyright= kn-copyright= en-aut-name=HanzawaAtsumasa en-aut-sei=Hanzawa en-aut-mei=Atsumasa kn-aut-name=半澤敦正 kn-aut-sei=半澤 kn-aut-mei=敦正 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=5-7 article-no= start-page=1299 end-page=1306 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=19600730 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Free Amino Acids in the Brains XIII A Study on the Free Amino Acids in Various Parts of Human Brain kn-title=脳の遊離アミノ酸について(XIII) ヒト脳各部位の遊離アミノ酸およびその関連物質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=By means of ion exchange column chromatography the author carried out quantitative analyses of 14 kinds of free amino acids including the related compounds using the brain of the person who died of acute loss of blood. The parts of the brain used for the analysis were frontal cortex, corpus callosum, caudate nucleus, globus pallidus, thalamus, hypothalamus and medulla oblongata. The results are as follows. 1. In corpus callosum which contains little cellular components extremely minute quantities of phosphoethanolamine, aspartic acid, glutamic acid and γ-aminobutyric acid could be detected. 2. In globus pallidus a surprisingly large quantity of γ-aminobutyric acid could be found and it was far greater than that contained in hypothalamus. 3. In medulla oblongata only small quantities of phosphoethanolamine, aspartic acid, and glutamic acid could be detected. Likewise γ-aminobutyric acid was found not so abundant. This seems to be due to the fact that the present experiment was conducted with medulla oblongata including white matter. 4. Although only in a small quantity, cystathionine could be assayed in all these parts except globus pallidus and thalamus. 5. Even from the comparative biochemistry the present quantitative analyses gave an interesting contrast to the values obtainable in the brains of lower animals. 6. Although it was difficult to recognize any distinct difference in the pattern of amino acids between the adult brain and the infant brain, there was a clear-cut difference in the amino acid pattern of the adult brain and that of the fetal brain. Namely, in the adult human brain there exist far greater quantities of aspartic acid, glutamic acid, γ-aminobutyrie acid, and N-acetylaspartic acid than those in the fetal brain and conversely far less quantities of phosphoethanolamine and taurine than in the latter. Likewise tyrosine detected in the fetal brain could not be recognized in the adult human brain. en-copyright= kn-copyright= en-aut-name=NishiokaHirosuke en-aut-sei=Nishioka en-aut-mei=Hirosuke kn-aut-name=西岡博輔 kn-aut-sei=西岡 kn-aut-mei=博輔 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=2 article-no= start-page=507 end-page=513 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=19600130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Transaminase of Brain (I) Transaminase Activities of the Monkey and Human Brain kn-title=脳のトランスアミナーゼ 第1編 タイワンザル脳およびヒト脳におけるトランスアミナーゼ活性について en-subtitle= kn-subtitle= en-abstract=The author measured the transaminase activities of eighteen amino acids with α-keto-glutarate in a Pithecus monkey brain and in two human brains, one obtained from a man died from accident and another from a woman died of senile psychosis due to brain atrophy. 1. In the monkey brain, alanine and valine showed low activities in the cerebral cortex in comparison with other parts but γ-amino-β-hydroxybutyric acid showed a low activity. 2. In normal human brain, lower activities of valine, leucine and three ω-amino acids were showed in the cerebral cortex, which were different from the monkey and lower vertebrates. 3. The markedly atrophic cerebral cortex was more similar to the lower vertebrates than to the normal human brain in high activities of alanine, leucine, valine and γ-amino-β-hydroxybutyric acid. But an increase of activities of histidine, proline and ornithine was not similar to both of normal human and animal brain. These high activities of histidine and praline correspond unlikely to the transamination only. kn-abstract=タイワンザル脳，事故死ヒト脳の大脳皮質，大脳白質および小脳皮質，さらに高度の脳萎縮を伴い老人性痴呆にて死亡したヒト脳の大脳皮質を用い， 11種のα-アミノ酸， 3種のω-アミノ酸， 3種のヂアミノ酸，システイン酸，タウリンについて，組織のトランスアミナーゼ活性を測定した. 1. タイワンザル脳では，大脳皮質においてアラニンとバリンの活性が他部より低く，γ-アミノ酪酸とγ-アミノ-β-オキシ酪酸が高い. 2. 健常人脳では，アスパラギン酸，セリンが大脳皮質に，ヒスチジン，ロイシン，バリンが小脳皮質にて他部より高く，アラニン，フエニールアラニン，システイン酸は大脳白質において低い活性を示した. 3. タイワンザルおよび健常ヒト脳の大脳皮質では，いずれもアスパラギン酸，グリシン，フエニールアラニン，オルニチン，システイン酸の高い活性，およびアラニンの低い活性の点で他の下等動物脳と異なる. 4. 健常ヒト大脳皮質は，バリン，ロイシンおよび3種のω-アミノ酸の低い活性を以つてサルおよび下等動物と異なる. 5. 萎縮したヒト大脳皮質は，アラニン，ロイシン，バリン，γ-アミノ-β-オキシ酪酸の活性が健常ヒト大脳皮質より高く，むしろ下等動物のそれに近い.ヒスチジン，プロリン，オルニチンのグルタミン酸生成の著しい増加は健常ヒト脳および下等動物脳のいずれとも異なるが，ヒスチジンとプロリンはトランスアミナーゼ活性増加のみによるものかどうか疑わしい. en-copyright= kn-copyright= en-aut-name=OnoMasaya en-aut-sei=Ono en-aut-mei=Masaya kn-aut-name=小野昌也 kn-aut-sei=小野 kn-aut-mei=昌也 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=7-8 article-no= start-page=665 end-page=676 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Enzyme Histochemistry of the Cerebellum with a special reference to α-glycerophosphate dehydrogenase in Purkinje cells and the problem of the fixation of the material kn-title=小脳の酵素組織化学―特にプルキンエ細胞のα-グリセロ燐酸脱水酵素と「固定の問題」をめぐつて― en-subtitle= kn-subtitle= en-abstract=With the cerebellum of albino rat, human and cat as the material the activities of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), and α-glycerophosphate dehydro-genase (α-GPDH) were studied histochemically. For the identification of SDH and α-GPDH, menadione (MD) was added to the incubation medium as an intermediator. In the freshly-prepares cryostat sections of the cerebellum, the activities of LDH and SDH, as has already been reported, have been found in the granular layer (glomeruli cerebellosi), molecular layer and Purkinje cells (P-cells), but the activity of α-GPDH has hardly been detected in P-cell layer. In contrast to this findings, when the tissue block was first fixed with cold formalin solution and then frozen sections were prepared, in the course of identification of α-GPDH the formation of Nitro-BT formazans was observed in P-cells. This fact in dicates that a special precaution is required in the selection of methods and the determination of results inthe enzyme histochemistry. From this point of view, the establishment of various experimental conditions optimal for such a study have been tried　with a specal reference to the problem of the fixation. The following results have been fouud with regard to the reaction in the sections fixed with formalin: 1) there is no marked difference due to the length of fixation time: 2) there is little effect due to the change in pH: 3) no specific inhibitory effect can be observed: 4) the presence of MD is prerequisite;　5) SH-group is involved; and 6) the enhancement of incorporation (adsorption; cf: substativity; Pearse) of Nitro-BT to the tissue is associated with the reaction. From these findings I have postulated that the reaction observable in the formalinfixed sections results from non-enzymic reaction in which SH-group and MD are involved, on the assumption that Nitro-BT is bound to the tissue protein. Furthevmore, the findings that P-cells do hardly exhibit the activity of α-GPDH in the non-fixed sections different from other cellular components of cerebellar tissue, as well as the fact that the non-enzymic recuction of Nitro-BT appears markedly in P-cells of the formalin-fixed specimen, seem to reflect one aspect of the cytochemical specificity of the P-cells. kn-abstract=(1) ダイコクネズミ及びヒト,ネコの小脳について,乳酸脱水素酵素(LDH),コハク酸脱水素酵素(SDH),α-グリセロ燐酸脱水素酵素(α-GPDH)活性を組織化学的に検索した. SDH,α-GPDHの検出にあたつてはintermediatorとしてmenadione (MD)を反応液に添加した. 新鮮凍結クリオスタット切片にて,LDHは顆粒層の“gloneruli cerebellosi”に相当して最も強く,分子層ではformazan顆粒はびまん性に形成されて反応は顆粒層よりやや弱くPurkinje細胞(P-細胞)は核を除いて細胞体に強度〜中等度の活性を示し,白質には活性はほとんど証明されなかつた. SDHは全体としてLDHの反応態度に似た像を示し,顆粒層に最も強く,ついでP-細胞層,分子層の順で活性が認められ,白質にはほとんど証明されなかつた. α-GPDH反応は,顆粒層,分子層においてはLDH, SDHとほぼ同じであるが,P-細胞層にはformazanの形成がほとんどなく,あたかも顆粒層と分子層の間に空隙がある様な像を示した.白質には活性はほとんど証明されなかつた,これらの所見は,これまでの報告にほぼ一致するものである. (2) 上記酵素活性の検出にあたり,新鮮凍結クリオスタット切片とともに,同一材料について組織塊をまず10%冷フォルマリンで固定した後に作製した凍結切片をあわせて使用した. SDH,α-GPDH, LDHともに反応は全般に減弱されるが,LDHの反応の減弱の度合は他の2者にくらべて少ないようであつた. ここで注目すべきことは,非固定切片のP-細胞層において,α-GPDHの活性がほとんど認められなかつたにもかかわらず固定切片のP-細胞には反応が認められ,しかもformazan形成は他の部よりもむしろ強度であつた点である.この事実は酵素組織化学における方法の選定や結果の判定にあたつては常に慎重な配慮が要求されることを示す現象であるとみなされ,この点に着目し特に固定の問題をめぐつて諸種の条件を実験的に設定してしらべた. (3) フォルマリン固定切片における反応には, i) 固定時間の長さによる著明な差異がみられない. ii) pHの変化による影響が少ない. iii) 特異的抑制剤による影響がみられない. iv) MDの存在が不可欠である. v) SH-基の関与がある. vi) Nitro-BTの組織への吸着性の増強が関連している. ということを認めた. このことからフォルマリン固定切片に見られる反応は,Nitro-BTの組織蛋白との結合性を前提とて,SH-groupとMDとが関与している非酵素的作用の結果であることを推論した. (4) 小脳組織でP-細胞が他の細胞要素と異なり,非固定切片においてα-GPDH活性をほとんど示さないこと,およびフォルマリン固定によりP-細胞に非酵素的なNitro-BT還元が著明にあらわれることは,P-細胞の細胞化学的特異性の一面を示すものであろう. en-copyright= kn-copyright= en-aut-name=TokunagaIwao en-aut-sei=Tokunaga en-aut-mei=Iwao kn-aut-name=徳永五輪雄 kn-aut-sei=徳永 kn-aut-mei=五輪雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 END start-ver=1.4 cd-journal=joma no-vol=79 cd-vols= no-issue=3-4 article-no= start-page=271 end-page=280 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=19670430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Malic Dehydrogenese Isozyme Part U. A Study on Malic Dehydrogenese Isozymes of Human Serum and Rat organs during Development kn-title=リンゴ酸脱水素酵素のアイソザイムに関する研究 第2編 ヒト血清及び発育過程におけるラット臓器のリンゴ酸脱水素酵素アイソザイムについて en-subtitle= kn-subtitle= en-abstract= kn-abstract=Malic dehydrogenese (MDH) activities in human serum of various diseases were studied. Elevation of MDH activities were observed in myocardial infarction, acute hepatitis, and some malignant tumors. Characteristic MDH isozyme patterns were obtained from serum of patients with myocardial infarction and acute hepatitis showing marked elevation of MDH activities. During the tetrazolium procedure for staining of electrophoreticaly separated serum malic or lactic dehydrogenese isozymes on agargel, without substrate and co-enzyme, two peaks were noticed and named serum "non-specific factor". These peaks were considered a kind of non specific reactions which resulted from a reduction of the tetrazolium salt electrostaticaly adsorbed on Alb. 〜 α(1), Glb. by SH-groups of these serum proteins. These reactions were accelerated by the elavation of PH or the temperature, exposure during staining and the prolonged staining time, as well as the rise of concentrations of NTB, PMS and CN- in the staining medium. Considering of optimal conditions of enzyme reaction, the minimum use of NTB, PMS and Na CN, washing stained agargels with PH 4.5 acetic acid SoL, and complete shading during procedure could minimize the influence of this factor. Two MDH isozymes were demonstrated in agargel isozymograms of rat organs, one migrating towards the cathod and the other toward the anode. The cathodal fraction was considered mitochondrial MDH (m-MDH), and the anodal one cytoplasmic MDH (c-MDH), respectively. During development, m-MDH showed marked increase in heart muscle. In contrast to this, cMDH of liver and gastric mucosa increased in procedure of development. On the other hand the ratio of m-MDH and c-MDH in kidney remained constant through development. en-copyright= kn-copyright= en-aut-name=TakayasuMasao en-aut-sei=Takayasu en-aut-mei=Masao kn-aut-name=高安正雄 kn-aut-sei=高安 kn-aut-mei=正雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=79 cd-vols= no-issue=3-4 article-no= start-page=259 end-page=270 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=19670430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Malic Dehydrogenese Isozyme Part T. A Study on Malic Dehydrogenese Activities and Isozymes of Various Human Organs kn-title=リンゴ酸脱水素酵素のアイソザイムに関する研究 第1編 ヒト各種臓器のリンゴ酸脱水素酵素活性及びアイソザイムについて en-subtitle= kn-subtitle= en-abstract= kn-abstract=Malic dehydrogenese (MDH) activities and isozymes of various human organs were measured. The assays of isozyme were carried out by means of agargel electrophoresis. The highest MDH activity was demonstrated in heart muscle. Liver, skeletal muscle, kidney and brain showed considerably high MDH activities. Six fractions were distinguished in many MDH isozymograms. In order of movility towards the cathod, each MDH isozyme was named MD(1), MD(2)…MD(6). Intracellular distribution of each MDH isozyme was investigated by means of cell fractionation, and MD(1)〜MD(5) were confirmed mitochondrial, MD(6) cytoplasmic, in origin. MDH isozyme-patterns of human organs were divided into 3 groups according to the ratio of mitochondrial MDH (m-MDH) and cytoplasmic MDH (c-MDH). Group T (m-MDH>c-MDH): heart muscle, kidney, skeletal muscle, white blood cell, red blood cell and lung-tissues. Group U (m-MDH≒c-MDH): liver, pancreas. Group V (This group situates between Group T and Group U): brain, gastric mucosa, and spleen. Studies on inhibitory effect of p-chlor-mercuri-benzoate (PCMB) on each MDH isozyme and heat stability of each MDH isozyme demonstrated that m-MDH was not inhibited by PCMB and stable to a heat test at 50℃ for 30,' when 1-malate was used as substrate. It was also observed that m-MDH was activated by a high concentration of 1-malate. According to the standard deviation of MD(6) and MD(3) activites, c-MDH considered regulatory enzyme and m-MDH constitutive enzyme, respectively. en-copyright= kn-copyright= en-aut-name=TakayasuMasao en-aut-sei=Takayasu en-aut-mei=Masao kn-aut-name=高安正雄 kn-aut-sei=高安 kn-aut-mei=正雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=82 cd-vols= no-issue=5-6 article-no= start-page=295 end-page=317 dt-received= dt-revised= dt-accepted= dt-pub-year=1970 dt-pub=19700630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Evoked Cortical Responses after the Stimulation of Specific and Unspecific Nuclei of the Thalamus of the Human and Cats kn-title=ヒトおよびネコの視床核電気刺激による皮質誘発反応に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cortical evoked responses after single or repetitive stimulations of the nucleus ventralis lateralis (VL), n. centrum medianum (CM) and n. ventralis anterior (VA) were observed in 61 cases of parkinsonism, 7 cases of intractable pain and a case of dystonia musculorum deformans from February, 1968 to December, 1969. Experimental studies of the thalamic stimulations with 15 cats were performed as well and the results were compared with that of the human. Evoked responses were analysed with averaging computer technique. Results were as followings. 1) Cortical evoked potentials after single stimulation of the VL nucleus of the human thalamus. a) Evoked potentials appeared on the ipsilateral centro-frontal regions with weaker stimulation in EEG records. However, the evoked potentials showed clear bilateral distribution except the temporal region, predominantly on both centro-frontal regions, with stronger stimulation electroencephalogaphically. b) The evoked potentials of the central site showed clear three negative waves within 200 msec after the stimulation, that is, the first negative wave (T-N) with the peak time of about 10 msec, the second (positive-) negative wave (U-PN) with the peak time of about 50 msec and the third negative wave (V-N) with that of 80-100 msec with averaging computer records. The peak time of the T-N wave and the negative phase of the U-PN wave were nearly constant, but that of the V-N wave considerably varied according to the background activities of EEG. The evoked potentials showed no significant discrepancy between right and left patterns, and moreover, they maintained a similar pattern when the stimulating voltages were changed. c) Bilateral evoked potentials were clearly seen with averaging computer technique even when evoked potentials were small and hardly identified on the contralateral scalp with EEG records. When the stimulating voltages were gradually decreased, the ipsilateral and contralateral evoked potentials disappeared approximately at the same time. d) The latency of the T-N wave was measured to be 5-6 msec with averaging computer records, and the amplitude of the T-N wave increased proportionately with the increase of stimulating voltages. en-copyright= kn-copyright= en-aut-name=NambaShimpei en-aut-sei=Namba en-aut-mei=Shimpei kn-aut-name=難波真平 kn-aut-sei=難波 kn-aut-mei=真平 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=11-12 article-no= start-page=575 end-page=593 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19721230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Fine Structure of Human Normal Gall Bladder and Cholesterosis kn-title=ヒト胆嚢粘膜及び胆嚢コレステローシスの電子顕微鏡的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Human gall bladders specimens obtained soon after cholecystectomy from 50 patients were observed under electron microscopy. Four types of cells were distinguished by light and electron microscopy in the human gall bladder epithelium, i.e.,"Ordinary epithelial cell", "Clear cell", "Dark cell", and" Basal cell". The Clear cell have been subdivided at least two types by electron microscopy. One was degenerative type and another was immatured one. It was also observed that the mechanism of discharge of secretory granules was the" Merocrine mode". In the cholesterosis, it was assemed that cholesterol was absorbed into the epithelial cells from the bile and accumlated in their basal area, then discharged through their basis. It was taken by phagocytes which gradually transformed into the foamy cells. en-copyright= kn-copyright= en-aut-name=TakahashiKimio en-aut-sei=Takahashi en-aut-mei=Kimio kn-aut-name=高橋公雄 kn-aut-sei=高橋 kn-aut-mei=公雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第T外科教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=11-12 article-no= start-page=395 end-page=396 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19721230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=低カタラーゼ血液症の簡易検出法に関する研究 第2編 ヒト血液凍結保存によるカタラーゼ活性度の変動について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=高越良明 kn-aut-sei=高越 kn-aut-mei=良明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=11-12 article-no= start-page=391 end-page=393 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19721230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=低カタラーゼ血液症の簡易検出法に関する研究 第1編 ヒト血液保存によるカタラーゼ活性度の変動について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=高越良明 kn-aut-sei=高越 kn-aut-mei=良明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=9-10 article-no= start-page=247 end-page=266 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19721030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Thalamo-cortical Relationship in Evoked Cortical Responses Following Human Ventrolateral Thalamic Stimulation kn-title=Thalamo-cortical Relationに関する電気生理学的研究-ヒト視床腹外側核刺激による皮質誘発反応の分析- en-subtitle= kn-subtitle= en-abstract= kn-abstract=Electrophysiological studies on evoked cortical potentials following ventrolateral (VL) thalamic stimulation of man were performed with averaging computer technique for 41 patients of parkinsonism and other involuntary movement disorders during stereotaxic surgery for last 2 years. 1. Single stimulation of the VL nucleus demonstrated bilateral cortical activity with I-PN (first positive negative), U-PN (second positive negative), V-PN (third positive negative) and W-PN (fourth positive negative) waves. V-P wave was frequently superimposed on negative response between U-N and V-N wave, and W-PN waves were frequently feeble in single stimulation of the VL nucleus. 2. Peak latencies of these waves were estimated 3.4±1.2 msec in T-P, 10.6±1.8 msec in T-N, 29±5 msec in U-P, 55±6 msec in U-N, 71±11 msec in V-P, 90±11 msec in V-N, 115±19 msec in W-P and 160±24 msec in W-N wave in ipsilateral central lead, and 4.0±1.5 msec in T-P, 11.1±2.1 msec in T-N, 31±4 msec in U-P, 57±6 msec in U-N, 74±13 msec in V-P, 92±12 msec in V-N, 118±24 msec in W-P and 156±29 msec in W-N wave in contralateral central lead. 3. The first deflection time of T-P wave, which meant beginning of the evoked response, was 1.5-1.8 msec in stimulated side of central cortex and 2.2-2.9 msec in contralateral central lead, which were obtained in 5 cases precisely measured. 4. The impulse, which provoked T-P N waves, was thought to be conducted from stimulated VL nucleus to the contralateral cortex directly via corpus callosum with 36-43m/sec of velocity. 5. Cortical evoked responses following suprathreshold low frequency stimulation (5-12Hz) of the VL nucleus showed invariably augmenting response and recruiting-like augmenting response. Augmenting response, which consisted of a train of growth in both. positive and negative components, was evoked when each stimulus was given on the descending phase from the peak of V-N or augmented negativiy to the bottom of following positive wave of the preceding response. Analysis of augmentation suggested that synchronization of preceding evoked W-P and present U-P wave would be occured in augmented positivity and synchronization of preceding evoked W-N and present U-N or V-N wave would be occured in augmented　negativity. Recruiting-like augmenting response was obtained when each stimulus was given on the ascending phase from the bottom of deep W-P to the following negative wave of the preceding response. Recruiting-like augmentation was shown to be a similar response as augmenting response in the fundamental pattern of averaged evoked activity, although development of negativity and attenuation of positivity caused recruiting-like pattern. Component analysis of recruiting-like augmenting response revealed that predominant development of negativity was thought to be the result of the synchronization of preceding evoked W-N and present U-N or V-N wave. And attenuation of U-P wave was thought to be the result of the desynchronization of preceding W-N and present U-P wave. Responses following lower frequency stimulation with 4Hz or less were similar to responses following single stimulation. Subthreshold stimulation were thought to be difficult to induce any growth of negative cortical response. 6. It was clarified that cortical evoked response following stimulation of the VL nucleus was influenced by components and phases of the preceding cortical response, suggesting that human specific thalamic system would play a possible important role in modulation upon cortical electrical activity. en-copyright= kn-copyright= en-aut-name=MiyamotoToshihiko en-aut-sei=Miyamoto en-aut-mei=Toshihiko kn-aut-name=宮本俊彦 kn-aut-sei=宮本 kn-aut-mei=俊彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科学教室 END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=7-8 article-no= start-page=403 end-page=408 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=19740830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cultivation of Human Lymphoblastoid Cells in Diffusion Chambers Implanted in the Abdominal Cavity of MiceU. Propagation of Rauscher Leukemia Virus to HumanLymphoblastoid Cells kn-title=マウス腹腔内な挿入されたdiffusion chamber内でのヒトリンパ芽球様株細胞の培養 第2編 ヒトリンパ芽球様株細胞へのRauscher白血病ウィルスの感染 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Diffusion chambers containing EBV-bearing human lymphoblastoid cells were placed temporarily within the peritoneal cavity of BALB/c mice with Rauscher leukemia. Reestablished human leukocyte cultures became dually infected with C type and EBV particles and have continuously produced abundant C type particles. These virus particles were nonleukemogenic when inoculated into BALB/c mice but afforded some protection against challenge infection with spleen-derived leukemogenic RLV. en-copyright= kn-copyright= en-aut-name=YoshimotoShuko en-aut-sei=Yoshimoto en-aut-mei=Shuko kn-aut-name=吉本修子 kn-aut-sei=吉本 kn-aut-mei=修子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科 END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=7-8 article-no= start-page=393 end-page=401 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=19740830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cultivation of Human Lymphoblastoid Cells in Diffusion Chambers Implanted in the Abdominal Cavity of Mice T. Morphologic Alteration of Human Lymphoblastoid Cells kn-title=マウス腹腔内に挿入されたdiffusion chamber内でのヒトリンパ芽球様株細胞の培養 第1編 ヒトリンパ芽球様株細胞の形態学的変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A clonal lymphoblastoid cell line derived from lymph node of a patient with Hodgkin's diseasewas cultivated in diffusion chambers implanted in the abdominal cavity of mice and cellular morphologic changes were sequentially studied for up to 8 weeks. The majority of cells before intraperitoneal implantation were lymphoblasts with approximately 16% mature lymphocytes. However, after 3 weeks of in vivo cultivation in diffusion chambers, macrophage-like cells began to increase and occupied 80-90% of cells after 4 and 5 weeks. These macrophage-like cells, when tested for phagocytic activity after 4 weeks, exhibited bacterial phagocytosis. Labeling studies with (3)H-thymidine indicated that active DNA synthesis continued for up to 2 weeks of in vivo diffusion chamber cultivation, during which mitotic figures were also observed morphologically. Further, it was attempted to recultivate in vitro cells that were taken out of diffusion chambers after 2, 3, and 4 weeks of in vivo implantation. These 3 separate attempts led to the development of lymphoblastoid cells that were morphologically and cytogenetically similar to those prior to diffusion chamber implantation. From these findings, it is suggested that human lymphoid cells undergo morphologic alteration to macrophages under certain circumstances. en-copyright= kn-copyright= en-aut-name=YoshimotoShuko en-aut-sei=Yoshimoto en-aut-mei=Shuko kn-aut-name=吉本修子 kn-aut-sei=吉本 kn-aut-mei=修子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科 END start-ver=1.4 cd-journal=joma no-vol=83 cd-vols= no-issue=11-12 article-no= start-page=569 end-page=578 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=19711230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of lymphoblastoid cell lines derived from human peripheral blood leukocytes. Part 11. Immunofluorescence study kn-title=ヒト末梢血由来リンパ芽球様株細胞に関する研究 第2編 蛍光抗体法による研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Three types of investigations by the immunofluorescence method were performed. Antibody to EB virus (EBV) in various sera and EBV antigen in the nine lymphoblastoid cell lines were tested by the indirect method and production of immunoglobulin in these cell lines was by the direct method. 1) It has been shown that the virus is widely disseminated among normal persons in Japan. 2) Newborn infants possess EBV antibody on delivery. This antibody is probably transmitted from mother to fetus via the placenta. 3) There is no difference in EBV antibody titers between normal individuals and donors from whom the lymphoblastoid cell lines were established. 4) EBV antigen appears in a small number of lymphocytes and macrophages within a few weeks after initiation of peripheral leukocyte cultures. 5) All the established cell lines persistly demonstrate a few percentage of EBV antigen-positive cells. 6 ) All the cell lines produce two or three classes of immunoglobulin but the proportion of the stained cells varies considerably during long-term culture. en-copyright= kn-copyright= en-aut-name=HasegawaHarumi en-aut-sei=Hasegawa en-aut-mei=Harumi kn-aut-name=長谷川晴巳 kn-aut-sei=長谷川 kn-aut-mei=晴巳 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科 END start-ver=1.4 cd-journal=joma no-vol=83 cd-vols= no-issue=11-12 article-no= start-page=553 end-page=567 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=19711230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of lymphoblastoid cell lines derived from human peripheral blood leukocytes. Part 1. Establishnent of 9 new cell lines kn-title=ヒト末梢血由来リンパ芽球様株細胞に関する研究 第1編 リンパ芽球様株細胞の樹立 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Nine new lymphoblastoid cell lines have been established from peripheral blood leukocytes of patients with infectious mononucleosis, subacute myelo-optico-neuropathy (SMON), acute myelogenous leukemia and chronic lymphatic leukemia. All the cell lines grow in suspension and mostly consist of lymphoblastoid cells regardless of the cell source and clinical status of the donors. A few percentage of Epstein-Barr virus (EBV) antigen-positive cells were detected by the immunofluorescence method in all the cell lines. These cell lines have maintained karyotypes of predominantly normal diploid modes. It is suggested that the origin of these cell lines may be certain lymphocytes in peripheral leukocytes. From the present study, a following hypothesis may be offered on the establishment of the lymphoblastoid cell lines. It is conceivable that EBV which is harbored in vivo in lymphocytes might be activated and replicate in the early stage of leukocyte culture. The virus would, thereafter, exert an antigenic stimulation on some of the lymphocytes and consequently they would undergo blastoid transformation, yielding self-sustaining cell lines. en-copyright= kn-copyright= en-aut-name=HasegawaHarumi en-aut-sei=Hasegawa en-aut-mei=Harumi kn-aut-name=長谷川晴巳 kn-aut-sei=長谷川 kn-aut-mei=晴巳 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科 END start-ver=1.4 cd-journal=joma no-vol=83 cd-vols= no-issue=7-8 article-no= start-page=247 end-page=258 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=19710830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cytological Studies on the Endocrine Cells in the Mucosa of Human Stomach　Part U. Histochemical and Ultrastructual Studies on the Endocrine Cells in the Pathological Antral Mucosa of Human Stomach kn-title=ヒトの胃の内分泌細胞の細胞学的研究 第U編 病的状態の胃前庭部粘膜における内分泌細胞の組織化学的・電子顕微鏡的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Normal and pathological antral mucosa (gastric ulcer, gastroduodenal ulcer, duodenal ulcer, gastric cancer, giant rugae, gastritis chronica and gastric polyp) were examined histochemically and electron microscopically. 1) It was clarified that the Ec-cells are observed only in the mucosa with intestinal metaplasia, and never in the normal mucosa. We tentatively classified the Ec-cells into two types; Ec-T-cell and Ec-U-cell. The specific granules in the Ec-T-cells are polymorphous and contain a dense core enclosed by a limiting membrane which fits either tightly or loosely. The Ec-U-cells have electron dense and polymorphous granules but do not contain a dense core in the limiting membrane. The ratio of Ec-T-cells to Ec-U-cells in the antral mucosa with intestinal metaplasia seems to be almost equal in gastric ulcer but smaller in gastric cancer. 2) Cell type W-cell in the normal antral mucosa has three kinds of granules; electron dense granules (100-200 mμ in diameter), granules (about 400 mμ in diameter) which have cloud-like or bubble-like substances in the limiting membrane, and granules which are empty-looking in the limiting membrane. In gastric ulcer cases, the W-type cells were apt to be granulated but there were no increse in the number. On the contrary, in atrophic gastritis, the W-type cells had low electron dense granules which were empty-looking in the limiting membrane. en-copyright= kn-copyright= en-aut-name=NabeyamaAkira en-aut-sei=Nabeyama en-aut-mei=Akira kn-aut-name=鍋山晃 kn-aut-sei=鍋山 kn-aut-mei=晃 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1外科教室 END start-ver=1.4 cd-journal=joma no-vol=83 cd-vols= no-issue=7-8 article-no= start-page=235 end-page=245 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=19710830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cytological Studies on the Endocrine Cells in the Mucosa of Human Stomach Part T. Ultrastructual Classification of Endocrine Cells in the Normal Antral Mucosa of Human Stomanch kn-title=ヒトの胃の内分泌細胞の細胞学的研究 第T編 ヒトの正常胃前庭部粘膜にみられる内分泌細胞の電子顕微鏡的分類 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The endocrine cells in te normal antral mucosa of the human stomach were examined by the electron microscope. According to their fine structural characteristics, they were classified into the following five types. 1) Cell-type U is triangular in shape. The granules are round and vary in diameter (300-600mμ) and in electron density. 2) Cell-type V is triangular in form and has many grnules (130mμ in diameter) with high electron density. These granules are characterized by their uniform size and homogeneous appearance. 3) Cell-type W contains a prominent endoplasmic reticulum in the supranuclear region. These kinds of granules are recognizable in the basal part: smaller ones (100-200mμ), which are dense, larger ones (400mμ) which are pale and another larger ones (400mμ) which are empty-looking. 4) Cell-type Xis contact with the glandular lumen and contains numerous secretory granules with various densities. 5) Cell-type Y is oval in shape and characterized by small granules (100-150mμ) with moderate density, localized in the basal region. This cell type seems to have some features of an immature endocrine cells. en-copyright= kn-copyright= en-aut-name=NabeyamaAkira en-aut-sei=Nabeyama en-aut-mei=Akira kn-aut-name=鍋山晃 kn-aut-sei=鍋山 kn-aut-mei=晃 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1外科教室 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=7-8 article-no= start-page=373 end-page=385 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Mechanism of Cortical Synchronized Activities with Special Reference to the Ventrolateral Thalamic Nucleus kn-title=大脳皮質電気活動の同期化における視床腹外側核の関与について-ヒトとネコにおける電気生理学的研究- en-subtitle= kn-subtitle= en-abstract= kn-abstract=Spindle-like afterdischarges evoked by stimulation of ventrolateral thalamic nucleus and spontaneous spindle waves were analized to investigate the electrophysiological mechanism of cortical synchronized activities in man as well as in cat. In man, three positive and three negative waves, namely T-P, T-N, U-P, U-N, V-P and V-N, were observed as cortical evoked responses after single stimulation of the ventrolateral thalamic nucleus. And W-P and W-N were observed after the stronger stimulation which could provoke augmenting response with the repetitive volley. Later components of evoked cortical responses were investigated in cat immobilized under local anesthasia. Recording electrole was located on the anterior sigmoid gyrus. As the intensity of the thalamic stimulation was increased, afterdischarges of the negative waves, which appeared with long latency (about 200 msec.), increased in number and developed into spindle-like pattern with notches on afterdischarges. The long latencied negative wave in cat and W-N in man were considered to correspond to Lehtinen's "precursor of the evoked spindle activity". It has been already reported by Miyamoto that W-N was supposed to play an important role in augmenting response in man. Close relationship was indicated between augmenting response and spindle-like afterdischarges. Futhermore, the similarities of the pattern between spindle-like afterdischarges following thalamic stimulation and spontaneous spindle waves in cat, and attenuation of ipsilateral spontaneous spindle waves after destruction of the ventrolateral thalamic nucleus in cat as well as in man were also observed. From these facts these cortical synchronized activities, namely AR, spindle-like afterdischarges and spontaneous spindle waves were considered to be brought by similar neuronal mechanism with some varieties of synchronization ascribed to arousal levels under influences of activities of the ventrolateral thalamic nucleus. On the other hand, spindle-like afterdischarges and spontaneous spindle waves in cat were analized for investigating their waxing and waning phenomena. Notches were observed on each waves. When the wave grew with maximal amplilude in a train of spindle, the notch of the wave became obscure. The phenomenon was considered that the notch was synchronized with the wave, forming maximal amplilude of the wave in a train of spindle. In other words, mechanism of this phenomenon was supposed to be explained as a beat of two rhythmic activities slightly different in frequency. en-copyright= kn-copyright= en-aut-name=BeckHiroichi en-aut-sei=Beck en-aut-mei=Hiroichi kn-aut-name=別宮博一 kn-aut-sei=別宮 kn-aut-mei=博一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学脳神経外科教室 END start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=5-6 article-no= start-page=223 end-page=229 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=19730630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cytotoxic effect of sensitized apleen lymphocytes with SV40 and SV40 induced tumor on SV40 transformed cells kn-title=SV40ウイルス及びSV40誘発腫瘍細胞により感作されたリンパ球のSV40変異ヒト胎児細胞に対する細胞障碍作用 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sensitized lymphocytes have been demonstrated to inhibit tumor growth in vivo systems. In SV40-viral oncogenesis, some specified immunization with virns and with virus-induced tumors to the animals results in rejection of further transplantation of the tumor cells. To analyze these phenomena by our specially devised cytotoxicity test, the authors have studied cell mediated immunity for tumor specific transplantation antigen (TSTA) in SV40 viral oncogenesis. The results suggest that sensitized lymphocytes inhibit the growth of heterogenic SV40-transformed target cells as well as syngenic ones. And the lymphocytes from tumor-bearing animals have lower ability of the inhibition on the target cells, which may be explained that the lymphocytes in tumor bearing animals are under the condition of immunologically insufficient state. en-copyright= kn-copyright= en-aut-name=EgusaKuniyuki en-aut-sei=Egusa en-aut-mei=Kuniyuki kn-aut-name=江草国之 kn-aut-sei=江草 kn-aut-mei=国之 aut-affil-num=1 ORCID= en-aut-name=SatoKeiko en-aut-sei=Sato en-aut-mei=Keiko kn-aut-name=佐藤啓子 kn-aut-sei=佐藤 kn-aut-mei=啓子 aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name=小田琢三 kn-aut-sei=小田 kn-aut-mei=琢三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌研生化学 affil-num=2 en-affil= kn-affil=岡山大学医学部癌研生化学 affil-num=3 en-affil= kn-affil=岡山大学医学部癌研生化学 END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1-2 article-no= start-page=97 end-page=100 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=19760228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Structure of the thoracic duct wall in human kn-title=ヒトの胸管壁の構造について en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the human thoracic duct wall the direction and the ascending angle of the smooth muscle cells are not necessarily fixed according to the site, but as a rule the smooth muscle cells of the interior layer of the tunica media ascend longitudinally at about 80° with their origin at the internal elastic membrane and connect to the circular muscle of tunica media that ascends at angle of about 10° and terminates at the external elastic membrane. At the exterior side of tunica media it becomes often longitudinal and sometimes it forms the exterior layer. The smooth muscle cells which connect the middle layer and exterior layer from the interior layer run in a specific arc line. In the case where a pair of valves cover the thoracic wall, at the site between the two valves there can be observed no longitudinal muscle of the interior layer. Generally the internal elastic membrane is incomplete, and often it forms longitudinal networks, but there is sometimes the case where morphologically it forms a well arranged internal elastic membrane. en-copyright= kn-copyright= en-aut-name=SuwaKiichi en-aut-sei=Suwa en-aut-mei=Kiichi kn-aut-name=諏訪喜一 kn-aut-sei=諏訪 kn-aut-mei=喜一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一解剖学教室 END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=11-12 article-no= start-page=1023 end-page=1032 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=19751230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=U. Histological Studies on Experimental Allergic Hepatitis with Appraisal of Mononuclear Cells in its Development kn-title=実験的アレルギー性肝炎の研究 第2編 同種肝抽出液感作リンパ球の移入による肝の組織変化 en-subtitle= kn-subtitle= en-abstract=In an attempt to evaluate the significance of mononuclear cells in the experimental allergic hepatitis which was previously studied, lymphocytes from inbred strain C(57) BL mice with the hepatitis was passively transfered into x-ray irradiated isologous mice. Early histological changes in the liver of the recipient mice consisted of an extensive coagulation necrosis on the 2nd or 3rd day after the transfer, and delayed changes occurring on the 7th to 10th post-transfer day included a marked infiltration of mononuclear cells in the portal areas, various types of liver cell necrosis and degeneration, a mononuclear cell infiltration in the sinusoid as well as an activation of the Kupffer cells as compared with control groups. These histological changes, however, were transient and subsided in 2 to 3 weeks post to the transfer of the cells. There was no tendency to the development of a feature of chronic hepatitis. The present experiment indicated that the passive transfer of sensitized lymphocytes obtained from mice with the experimental allergic hepatitis made by the previous experiment produced an allergic hepatitis comprising a coagulation necrosis and others in isologous mice, but this hepatitic change was transient and by no means transformed to a chronic status similar to a human chronic hepatitis as in the previous experiment. kn-abstract=ヒトの肝炎の進展,慢性化の過程において,肝のグリソン鞘に浸潤している単核細胞がいかなる役割を果しているかを究明する一つの試みとして, complete Freund's adjuvant添加同種肝抗原でC57Blマウスを長期感作して実験的アレルギー性肝炎を作成し,感作C57Blマウスの脾およびリンパ節より感作リンパ球を分離し,レントゲン全身照射後の同系マウスに移入し,受動転嫁実験を実施した.その結果,対照群に比して,39例中15例に肝の組織変化を認めた.そのうち,早期すなわち移入後2〜3日目に発生した広範な凝固壊死を14例中3例に,やゝ遅れた時期すなわち移入後5〜8日目に発生したpiecemeal necrosisを主体とする変化を25例中2例に認め,それらの変化は感作リンパ球による肝細胞性壊死であることを強く示唆した.以上の肝の組織変化はヒトのウイルス性肝炎の組織所見に類似しており, B型肝炎発症における主要因の1つ,すなわちHB抗原感作リンパ球の果す意義にも関連して重要な実験成績であると考えた. en-copyright= kn-copyright= en-aut-name=HirataHiroaki en-aut-sei=Hirata en-aut-mei=Hiroaki kn-aut-name=平田弘昭 kn-aut-sei=平田 kn-aut-mei=弘昭 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科学教室 END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=11-12 article-no= start-page=1009 end-page=1021 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=19751230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=T. Histological Studies on Experimental Hepatitis after Sensitization with Homologous Liver Extract kn-title=実験的アレルギー性肝炎の研究 第1編 AKRマウス肝抽出液によるC57Blマウス長期感作後の組織学的変化 en-subtitle= kn-subtitle= en-abstract=In an attempt to evaluate histologically an allergic phenomenon involved in the development of chronic hepatitis in man, the inbred strain C57BL mice were sensitized for prolonged durations from 5 to 19 weeks with a homologous liver extract from AKR mice as well as with complete Freund's adjuvant. Histological changes in the liver of the sensitized mice immediately after the treatment comprised a marked round cell infiltration in the portal areas, piece-meal necrosis, various types of liver cell necrosis and degeneration, and an activation of the Kupffer cells as compared with control groups. These histological changes somewhat resembled the findings of chronic hepatitis in human liver disease, but they have greatly subsided in 5 weeks post to the last sensitization. It may be assumed on the basis of the present experiment that a persistent sensitization for a longer duration is necessitated for the establishment of a chronic murine hepatitis similar to a human chronic hepatitis. kn-abstract=ヒトのウイルス性肝炎の発症および慢性化のアレルギー性機序を解明するための一手段として, AKR肝抽出液を抗原としてC57Blに長期感作を施行し,肝グリソン氏鞘内に著明な円形細胞浸潤,piecemeal necrosis,肝細胞壊死,星細胞の反応を作成しえたが,リンパ?胞の形成,肝グリソン氏鞘の結合織の増生は認められなかった.以上の組織変化は肝炎B抗原,すなわち,　hetero抗原とその抗体とのアレルギー機序によって生じると考えられるヒトのウイルス性肝炎の組織表現にかなり類似している.しかし,長期感作を中止し放置すると,組織変化は消褪した. en-copyright= kn-copyright= en-aut-name=HirataHiroaki en-aut-sei=Hirata en-aut-mei=Hiroaki kn-aut-name=平田弘昭 kn-aut-sei=平田 kn-aut-mei=弘昭 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科学教室 END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=9-10 article-no= start-page=893 end-page=899 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=19751030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Infection of Human Cultured Leukocytes with Murine Rauscher Leukemia Virus 2. Heterotransplantation of a Human Lymphoblastoid Cell Line Chronically Infected with Epstein-Barr Virus and Rauscher Leukemia Virus kn-title=マウス白血病ウイルスのヒト培養白血球への感染に関する研究 第2編EBウイルスとC型ウイルスの重複持続感染系ヒトリンパ芽球様株細胞の異種移植 en-subtitle= kn-subtitle= en-abstract= kn-abstract=An Epstein-Barr virus (EBV) -positive human lymphoblastoid cell line chronically infected with Rauscher murine leukemia virus (RLV) was transplanted into antilymphocyte serum (ALS)- treated newborn hamsters. Four of 10 hamsters transplanted developed nonlethal regressive tumors which continued to shed C-type virus. Lymphoblastoid cell cultures reestablished from the heterotransplanted tumor cells were positive for both EBV capsid and murine gs-1 antigens. In contrast, all the hamsters transplanted with its parent cell line without RLV infection succumbed to lethal progressive tumors. The RLV-infected cells were shown to have cell surface antigen that was not found in the noninfected cells. It is postulated that new membrane antigen associated with C-type virus infection provoked more immunologic reaction even in ALS-treated hamsters, accounting for the reduced tumorigenicity of the RLV-infected human leukocytes. en-copyright= kn-copyright= en-aut-name=HayashiTakehiko en-aut-sei=Hayashi en-aut-mei=Takehiko kn-aut-name=林建彦 kn-aut-sei=林 kn-aut-mei=建彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=5-6 article-no= start-page=549 end-page=556 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=19750630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Infection of Human Cultured Leukocytes with Murine Rauscher Leukemia Virus T. Persistent Dual Infection of Human Lymphoblastoid Cells with EB Virus and C Type Virus kn-title=マウス白血病ウィルスのヒト培養白血球への感染に関する研究 第1編 ヒトリンパ芽球様株細胞におけるEBウイルスとC型ウイルスの重複持続感染の成立 en-subtitle= kn-subtitle= en-abstract= kn-abstract=An EB virus-carrying lymphoblastoid cell line established from peripheral blood of a patient with SMON was super-infected in vitro with murine Rauscher leukemia virus. Since then, the cell line has been dually infected with both viruses for more than 1.5 years, as repeatedly demonstrated by the presence of herpes type virus particles and C type virus particles. The C type virus particles liberated from the human cultured leukocytes were no longer leukemogenic when inoculated into otherwise susceptible BALB/c mice, but this procedure rendered them considerably resistant to challenge infection with leukemogenic Rauscher leukemia virus that had been maintained by passage through BALB/c mice. The infectivity of the human cell-derived C type virus to other lymphoblastoid cell lines established from different patients and to primary BALB/c mouse embryo cells could not be demonstrated by the method employed. The implication of these findings is discussed in the light of possible viral etiology of human malignancy. en-copyright= kn-copyright= en-aut-name=HayashiTakehiko en-aut-sei=Hayashi en-aut-mei=Takehiko kn-aut-name=林建彦 kn-aut-sei=林 kn-aut-mei=建彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=フコース除去抗体のヒト末梢血中における抗体依存性細胞傷害（ADCC）活性誘導作用及びその機序に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IidaShigeru en-aut-sei=Iida en-aut-mei=Shigeru kn-aut-name=飯田茂 kn-aut-sei=飯田 kn-aut-mei=茂 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=5-6 article-no= start-page=629 end-page=639 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Human embryonic liver cell in culture Part U. kn-title=ヒト胎児肝細胞の培養 第2報 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the previous report, we dealt with the method and the culture of human embryonic liver cells, and its difficulties in establishing epithelial cell lines from normal human liver. HuL-4 cell strain was published as an epithelial cell strain in the previous paper. We examined the cell morphologically, histochemically, and the cellular localisation of albumin and AFP (α-fetoprotein) immunofluorescently. Chromosome of the cells and uptake of Indian ink into the cell were also undertaken. We also calculated the growth curve of the strain and analyzed the liver specific enzymes. The results are summarized as follows. 1). HuL-4 cell strain derived from human embryonic liver disclosed dominant epithelial cell growth than fibroblastic cells, and it continued to cultivate up to 24 passages in 758 days. 2). Direct immunofluorescence method for albumin and AFP failed to show any positive stain. 3). PAS staining of the cell revealed weak positive, and it was digested by diastase. PAP silver staining was negative, and the uptake of Indian ink into the cells was negative for up to 12 hours. 4). Chromosome analysis of the cell was normal (46 numbers, diploid) and no marker chromosomes were detected. 5). Hul-4 cell strain was epithelial morphologically, but it contained no liver specific enzymes. en-copyright= kn-copyright= en-aut-name=ImaiHiromichi en-aut-sei=Imai en-aut-mei=Hiromichi kn-aut-name=今井寛途 kn-aut-sei=今井 kn-aut-mei=寛途 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部付属癌源研究施設病理部 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトデ・プロテインキナーゼCアイソフォームおよびCdc25プロテインホスファターゼに関する研究 kn-title=Studies of Starfish Protein Kinase C Isoforms and Cdc25 Protein Phosphatase en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MiyakeYasuo en-aut-sei=Miyake en-aut-mei=Yasuo kn-aut-name=三宅康夫 kn-aut-sei=三宅 kn-aut-mei=康夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=可逆性不死化ヒト骨髄間葉系幹細胞を用いた骨再生の検討 kn-title=Bone Repair by Transplantation of hTERT-Immortalized Human Mesenchymal Stem Cells in Mice en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakaharaHiroyuki en-aut-sei=Nakahara en-aut-mei=Hiroyuki kn-aut-name=中原啓行 kn-aut-sei=中原 kn-aut-mei=啓行 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=11-12 article-no= start-page=1597 end-page=1607 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19771230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Enzymic studies of breast cancer Part 2. Influences of hormonal incubation of human breast cancer tissue on glycolytic enzymic activity kn-title=乳癌の酵素活性に関する研究 第2編 ヒト乳腺組織の酵素活性ならびにヒト乳癌組織の酵素活性に及ぼすホルモンの影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Glycolytic enzyme activities of human breast cancer was studied on age, menopausal status, T, N, Stage and pathology in benign and malignant tumor. Influences on incubation with additional hormones also were studied. Mastopathy, benign tumor and breast cancer showed varied enzymic activity. High enzymic activity was observed in breast cancer tissue, but the enzymic activity was not paralleled with tumor size. Enzymic activity changed by incubation with additional hormones. Pre menopausal state, T(1), negative lymph node metastasis, Stage I tissues were elevated enzymic activity. Post menopausal state, T(3), positive lymph node metastasis, and Stage III tissues were decreased enzymic activity. en-copyright= kn-copyright= en-aut-name=InoueMakoto en-aut-sei=Inoue en-aut-mei=Makoto kn-aut-name=井上真 kn-aut-sei=井上 kn-aut-mei=真 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1外科教室 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=3-4 article-no= start-page=265 end-page=294 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Transmission and scanning electron microscopic studies on human chronic gastritis kn-title=ヒトの慢性胃炎胃粘膜の電子顕微鏡的走査型電子顕微鏡的観察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Thirty-six cases of human atrophic chronic gastritis were observed by transmission and scanning electron microscope. Generally, in the surface and neck mucous cells, vacuole formations and swelling of mitochondria were occasionally observed. Although, increase of young parietal cells were frequently noted, ultrastructural changes were hardly seen in the cytoplasm and the nucleus of both the parietal cells and the chief cells. Intestinal metaplastic mucosa was found in almost all cases of antral mucosa and in half cases of corporal mucosa of chronic gastritis. In the SEM observation, the gastric mucosa and the intestinal metaplastic mucosa were clearly bordered. In TEM observations, all types of normal small intestinal cells were found in the intestinal metaplastic mucosa, and there were no ultrastructural differences between normal intestinal cells and intestinal metaplastic mucosa cells. It was interesting that enterochromaffin cells, which were scarce in normal gastric mucosa and numerous in small intestine, were frequently observed in intestinal metaplastic mucosa. In the three cases of chronic gastritis, the peculiar type cells which had both endocrine cells granules and mucous granules were found and their origins were discussed. en-copyright= kn-copyright= en-aut-name=ImajoTamotsu en-aut-sei=Imajo en-aut-mei=Tamotsu kn-aut-name=今城保 kn-aut-sei=今城 kn-aut-mei=保 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1外科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=9-10 article-no= start-page=1329 end-page=1357 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19771030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A cytochemical study of the absorption of horseradish peroxidase through the human gastrointestinal tract kn-title=ヒト消化管からのHorseradish Peroxidaseの吸収の細胞化学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using horseradish peroxidase (HRP) which serves as its own tracers, the absorption of intact macromolecules through the human gastrointestinal tracts was observed. In healthy volunteers, HRP was poured into the stomach, the duodenum, and the ileum through the gastric, duodenal and colonofiberscopes at several days interval. Twenty minutes after the injection, small samples of mucosa were removed. The tissues thus obtained were treated in the manner of Karnovsky and embedded in Epon and observed under an electron microscope. The absorption of HRP through the human small intestine and the intestinal metaplastic epithelia of the stomach was clearly demonstrated. Moreover, in intestinalized epithelium of the stomach, HRP was absorbed and transferred into the capillary lumen. These results indicated the posibility of absorption of macromolecules through the human gastrointestinal tract, which may be a cause of food-allergy and food poisoning. At the same time, it showed the effectiveness of oral administration of some enzyme-containing medicines. The absorption through the intestinal metaplastic epithelium was considered to be especially significant, because eaten proteins are promptly absorbed before digestion occurred. en-copyright= kn-copyright= en-aut-name=MatsumotoTeruo en-aut-sei=Matsumoto en-aut-mei=Teruo kn-aut-name=松本昭郎 kn-aut-sei=松本 kn-aut-mei=昭郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1外科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=9-10 article-no= start-page=1221 end-page=1243 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19771030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A cytological study on the caveolated cells of the rat and the human digestive organs kn-title=ラットおよびヒトにおけるcaveolated cellの細胞学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Firstly, the ultrastructure of the caveolated cells on the rat bile duct were examined by a transmission and a scanning electron microscope. Next, the author seeked whether caveolated cells were present or not in the human digestive organs. Lastly, functional study of the caveolated cells on the rat bile duct were investigated using horseradish peroxidase and pilocarpine. Then the facts mentioned below were clarified. 1) Caveolated cells in the rat bile duct were found most densely at the divelticulum-like recesses but on the luminal surface except this recesses caveolated cells were found partly densely and partly sparsely. 2) On the rat, the straight filaments of the caveolated cells of the bile duct were less developed than that of the digestive tract, but circular filaments were more developed than that of the digestive tract. 3) The apical vesicles of the caveolated cells were revealed to have no communication to outer lumen of these cells using lanthanum as a tracer. 4) The caveolated cell-like cells were found in the mucosa of a human colon. But these cells could not be identified clearly with caveolated cells. Because the vesicles were scanty and small in size and the inner substance was relatively electron opaque. 5) After intraluminal injection of peroxidase into ligated segments of bile duct, the marker was found within the apical vesicles. But it was not uncertain whether pre-existing apical vesicles had taken up the marker or vesicles containing the marker were formed newly by pinocytosis. 6) After intraperitoneal injection of pilocarpine, the supranuclear vesicles began to expand and the vesicles with granules moved toward the free surface suggesting eruption or diacrine secretion. And frequently apocrine secretion was also observed. In conclusion, the author supposes that caveolated cell may have both absorptive and secretory functions and this type cells may present in human digestive tract. en-copyright= kn-copyright= en-aut-name=OkudaTakehiko en-aut-sei=Okuda en-aut-mei=Takehiko kn-aut-name=奥田武彦 kn-aut-sei=奥田 kn-aut-mei=武彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=7-8 article-no= start-page=1035 end-page=1047 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the distribution of lymphocyte subpopulations in human tissues Part 2. Identification of lymphocyte subpopulations infiltrating in autoimmune diseases kn-title=ヒト組織内リンパ球subpopulationの分布に関する研究 第2編 臓器内浸潤リンパ球subpopulahonの分布-自己免疫性疾患を中心として. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The lymphocyte subpopulations were detected in the target organs obtained from various autoimmune disorders on the tissue cryosections. Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) were detected by rosette formation with sheep erythrocytes (E) or with sheep erythrocytes coated with antibody and complement (EAC) respectively. Almost all infiltrating lymphocytes in the salivery glands in patients with Sjogren's syndrome were identified as B cells and T cells were detected only along the ducts. In Hashimoto's thyroiditis, infiltrating lymphocytes with the lymph follicles in the thyroid gland were mostly B cells whereas T cells localized as surrounded the thyroid follicles. T cells would not be detected in the spleen sections obtained from the patients with hypoplastic anemia at all. The white pulp was constituted by B cells, and the mononuclear cells in the red pulp might be macrophages of which these cell surfaces had IgG-Fc receptors. The lymph follicles of the thymus in a patient with myasthenia gravis complicated with thymoma was demonstrated to be formed with B cells. Therefore, it could be concluded that B cells were predominant lymphocytes in the target organs, while T cells localized in situ developing the autoimmune reactions in various autoimmune diseases. en-copyright= kn-copyright= en-aut-name=KoriyamaKenji en-aut-sei=Koriyama en-aut-mei=Kenji kn-aut-name=郡山健治 kn-aut-sei=郡山 kn-aut-mei=健治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=7-8 article-no= start-page=1021 end-page=1033 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the distribution of lymphocyte subpopulations in human tissues Part 1. Fundamental studies on identification of lymphocyte subpopulations and their application to lymphoid organs kn-title=ヒト組織内リンパ球Subpopulationの分布に関する研究 第l編 組織内リンパ球subpopulationの分布同定法の基礎的検討および各リンパ組織におけるその分布 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Thymus-derived lymphocytes (T-cells) and bone marrow-derived lymphocytes (B-cells) in the cryosection of human tissues were identified either by rosette formation with sheep erythrocytes (E) or with erythrocytes sensitized with antibody and complement (EAC), respectively. Invariable and stable reaction was obtained in EAC rosettes of the tissue lymphocytes while E binding of them was rather unstable. E could be adhered in the thymus sections which were obtained from normal infants during open heart surgery, but EAC did not bind to them at all. It was confirmed that B-cells located mostly in the lymph follicles of the lymph nodes as well as the palatine tonsils and T-cells were present predominantly in the paracortical area and interfollicular region. B-cells in the germinal centers would generally have more activated C(3) receptors than those of the mantle zones. In addition, B-cells of the lymph follicles both in the lymph nodes and in the palatine tonsils could be also detected utilizing the membrane immunofluorescence technique. The proportion of T and B-cells distribution in the lymphoid tissue was well correlated with that obtained on free cell suspension technique.Therefore, it could be concluded that T and B lymphocytes were able to identify respectively even in tissue level. en-copyright= kn-copyright= en-aut-name=KoriyamaKenji en-aut-sei=Koriyama en-aut-mei=Kenji kn-aut-name=郡山健治 kn-aut-sei=郡山 kn-aut-mei=健治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=7-8 article-no= start-page=949 end-page=953 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Pattern of immunoglobulin production in human lymphoblastoid cell lines kn-title=ヒトリンパ芽球様細胞株における免疫グロブリン産生の研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Membrane-associated (M-Ig) and cytoplasmic immunoglobulins (C-Ig) of 4 human lymphoblastoid cell lines and 3 clones isolated from one of them were examined by immunofluorescence test. It was found that individual cells produced only a single type of light chain components and one or more types of heavy chain components of Ig. There was no specific correlation between the Ig types of M-Ig and C-Ig. en-copyright= kn-copyright= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name=赤木忠厚 kn-aut-sei=赤木 kn-aut-mei=忠厚 aut-affil-num=1 ORCID= en-aut-name=MatsudaYuzo en-aut-sei=Matsuda en-aut-mei=Yuzo kn-aut-name=松田勇蔵 kn-aut-sei=松田 kn-aut-mei=勇蔵 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=高知県立中央病院:癌研究所 affil-num=2 en-affil= kn-affil=岡山大学医学部:第2内科 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=11-12 article-no= start-page=1131 end-page=1139 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19801230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Monocytes in patients with malignant diseases Part 2 kn-title=ヒト末梢血単球の検討 第2編 悪性腫瘍患者における末梢血単球数とlysosomal enzymeの検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Absolute peripheral blood monocyte counts and the β-galactosidase activity of monocytes were studied in 60 patients with lung cancer, 20 with malignant lymphoma, 17 with other malignant diseases, 13 with sarcoidosis and 28 normal individuals. The monocyte count was 296.3±176.8/cmm in lung cancer, 315.6±160.0/cmm in malignant lymphoma, 217.8±89.6/cmm in sarcoidosis, and 298.6±144.1/cmm in normal individuals. Although there was no significant differences in the monocyte count of malignant and non-malignant diseases, all patients with lung cancer or malignant lymphoma had monocyte counts less than 100/cmm. In patients with lung cancer and malignant lymphoma, there was no significant differences in histological types or clinical stages. The monocyte count in lung cancer patients treated with streptococcal agent OK-432 was elevated to 484.9±269.0/cmm, as compared with 315.5±107.7/cmm before administration. A nadir of monocyte counts was found at one week after combination chemotherapy but recovery to normal value was observed by 3 weeks after. On the other hand, white blood cell counts reached a nadir 2 weeks after chemotherapy and recovered by 4 weeks after. The percentages of β-galactosidase positive monocytes were 21.5±11.5% in patients with lung cancer, 20.1±7.5% in malignant lymphomas, 19.1±6.8% in other malignant diseases and 25.3±12.4% in normal individuals. There was no significant differences in β-galactosidase activity related to histological types or clinical stages of patients with lung cancer and malignant lymphoma. No changes in β-galactosidase activity were found between before and after combination chemotherapy. en-copyright= kn-copyright= en-aut-name=TakasugiKenta en-aut-sei=Takasugi en-aut-mei=Kenta kn-aut-name=高杉健太 kn-aut-sei=高杉 kn-aut-mei=健太 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第二内科学教室 en-keyword=悪性腫瘍 kn-keyword=悪性腫瘍 en-keyword=末梢血単球数 kn-keyword=末梢血単球数 en-keyword=β-ガラクトシダーゼ活性 kn-keyword=β-ガラクトシダーゼ活性 en-keyword=ライゾゾーム酵素活性 kn-keyword=ライゾゾーム酵素活性 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=11-12 article-no= start-page=1123 end-page=1130 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19801230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation of monocytes in human peripheral blood Part 1 by discontinuous density gradient kn-title=ヒト末梢血単球の検討 第1編 ヒト末梢血単球の分離と同定 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The isolation of monocytes in human peripheral blood by discontinuous density gradients was studied. Discontinuous density gradients were obtained by successive layering of bovine serum albumins into three layers in 10ml glass tubes. Buffy coat cells were layered on top of a gradient and centrifuged under various conditions of centrifugation. Optimal isolation was achieved by centrifugation on a three layer of 35-32-28% bovine serum albumin at 490g for 60 minutes. The purity, yield and recovery of monocytes at the interface between 35% and 32% bovine serum albumin were 54.0%, 7.0×10(5) and 22.0% respectively. After centrifugation on Conray-Ficoll mixtures, the interface fraction containing lymphocytes was removed and the bottom fraction containing monocytes and other cells was centrifuged under various condition. The optimal condition was on a three layer of 35-32-28% bovine serum albumin at 400g for 40 minutes. The purity of monocytes by this method increased to 75% but the yield and recovery declined to 2.5×10(5) and 4.5% respectively. From these data I concluded that isolation of monocytes from blood only on density gradients was difficult because of a partial overlap between monocytes and lymphocytes in the density distribution profiles. en-copyright= kn-copyright= en-aut-name=TakasugiKenta en-aut-sei=Takasugi en-aut-mei=Kenta kn-aut-name=高杉健太 kn-aut-sei=高杉 kn-aut-mei=健太 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 en-keyword=末梢血単球の分離 kn-keyword=末梢血単球の分離 en-keyword=比重遠沈法による末梢血単球の分離 kn-keyword=比重遠沈法による末梢血単球の分離 en-keyword=牛アルブミンによる末梢血単球の分離 kn-keyword=牛アルブミンによる末梢血単球の分離 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=5-6 article-no= start-page=679 end-page=691 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A new rapid assay method of collagenase activity and its clinical applications Part 2. Determination of collagenase activity in human leucocytes and synovial fluids by the newly developed method kn-title=コラゲナーゼ活性の新しい迅速測定法の検討とその応用 第2編 ヒト好中球および関節液コラゲナーゼ活性測定への溶液法の応用 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A newly developed collagenase assay method using (14)C-labeled soluble collagen as substrate has been applied to the determination of collagenase activity in human leucocytes and synovial fluids. The results indicated that collagenase activity in the both samples could be measured by this method as observed with tadpole collagenase, except that 35 % dioxane was preferable for the extraction of the enzyme digestion products in the case of synovial fluids. Collagenase activity determined by the present method was well correlated with that obtained by the conventional assay method using reconstituted collagen fiblils as substrate (r=0.963 and 0.829 (p<0.01) respectively). In preliminary experiments with leucocytes from patients with diabetes mellitus (DM), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), it was observed that collagenase activity in leucocytes increased significantly in SLE and from some patients with RA, while no appreciable differences were observed in diabetics, compared to that in normals. In synovial fluids from patients with RA and osteoarthritis (OA), the accumulation of large amount of inactive (latent) form of collagenase was demonstrated by the treatment of synovial fluids with 3M KI. And it was varied significantly in one patient at different time while no appreciable amount of the active form was observed during the period. en-copyright= kn-copyright= en-aut-name=TeratoKuniaki en-aut-sei=Terato en-aut-mei=Kuniaki kn-aut-name=寺戸国昭 kn-aut-sei=寺戸 kn-aut-mei=国昭 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第3内科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=5-6 article-no= start-page=609 end-page=613 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Identification of tyramine and octopamine in human serum by mass fragmentography kn-title=Mass fragmentographyによるヒト血清中tyramineおよびoctopamineの同定 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Tyramine and octopamine were identified by means of mass fragmentography in human serum. The peaks identical to the retention time of tyramine and octopamine were found on gaschromatogram obtained from serum sample. Two fragments, M/e 316 and M/e 303 in mass spectrum of tyramine were used in tyramine identification, and M/e 528 and M/e 515 were used in octopamine identification. As the experimental results, the ratio M/e 303 : M/e 316 of the sample was identical to that of authentic tyramine, and ratio M/e 515 : M/e 528 was identical to that of authentic octopamine. These results suggest that tyramine and octopamine could exist in human serum. en-copyright= kn-copyright= en-aut-name=OkitaMisako en-aut-sei=Okita en-aut-mei=Misako kn-aut-name=沖田美佐子 kn-aut-sei=沖田 kn-aut-mei=美佐子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳代謝研究施設病態生化学部門 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=5-6 article-no= start-page=775 end-page=785 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on human leukemic B-cell line Part U. Serial transplantation of human leukemic B-cell line (BALL-1) into hamsters kn-title=ヒト白血病性B細胞株に関する研究 第2編 ヒト白血病性B細胞株（BALL-1株）のハムスターへの継代移植 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A human leukemic B-cell line, which was designated as BALL-1, was recently established in our laboratory. In this experiment, BALL-1 cells were serially transplanted into antilymphocyte serum (ALS)-treated newborn Syrian hamsters. When 1.0-8.5×10(7) viable cells were inoculated intraperitoneally, all of them developed invasive tumors 12-26 days after implantation. All the hamsters except one had 0.2-8.0ml of milky ascites containing 0.9-11.0×10(8) tumor cells/ml. Histologically, invasion of the tumor cells was observed in many abdominal organs and in subcutaneous tissue. Progression to leukemia was common (24/25), and the percentage of the tumor cells in the peripheral blood ranged between 2 and 91% . Morphological features, surface markers, and chromosome constitutions of the tumor cells were identical to those of BALL-1 cells. These findings show thast BALL-1 cells were serially transplantable into the experimental animals possessing the characteristics of human leukemic B-cells. To our knowledge, this is the first report of successful heterotransplantation of a human leukemic B-cell line. This animal model will provide a new advantage in many aspects of leukemia research. en-copyright= kn-copyright= en-aut-name=NakamuraKazuo en-aut-sei=Nakamura en-aut-mei=Kazuo kn-aut-name=中村一夫 kn-aut-sei=中村 kn-aut-mei=一夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=human acute lymphoblastic leukemia kn-keyword=human acute lymphoblastic leukemia en-keyword=leukemic B-cell line kn-keyword=leukemic B-cell line en-keyword=heterotransplantation kn-keyword=heterotransplantation END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=5-6 article-no= start-page=763 end-page=773 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on human leukemic B-cell line Part I. Establishment and characterization of human leukemic B-cell line (BALL-1) kn-title=ヒト白血病性B細胞株に関する研究 第1編 ヒト白血病性B細胞株(BALL-1株)の樹立とその性状 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A human B-cell line designated as BALL-1 was established from the peripheral blood of a patient with acute lymphoblastic leukemia (ALL). Neither Epstein-Barr virus (EBV) nor its genome was detectable. The morphological and growth characteristics were clearly distinct from those of numerous EBV-positive lymphoblastoid cell lines previously reported. BALL-1 cells probably originated from the donor's leukemic cells judging from their cytogenetic, morphological and surface features. This BALL-1 line is the first EBV-negative B-cell line established from ALL. en-copyright= kn-copyright= en-aut-name=NakamuraKazuo en-aut-sei=Nakamura en-aut-mei=Kazuo kn-aut-name=中村一夫 kn-aut-sei=中村 kn-aut-mei=一夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=human acute lymphoblastic leukemia kn-keyword=human acute lymphoblastic leukemia en-keyword=tissue culture kn-keyword=tissue culture en-keyword=leukemic B-cell line kn-keyword=leukemic B-cell line END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=3-4 article-no= start-page=301 end-page=309 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on a human leukemic null cell line Part T. Establishment of a human leukemic null cell line kn-title=ヒト白血病性null細胞株に関する研究 第I編 急性リンパ性白血病患者よりのnull細胞株の培養樹立 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A new cell line (NALL-1) was established in vitro from the peripheral blood of a patient with acute lymphoblastic leukemia (ALL). NALL-1 cells had neither properties of T and B cells nor Epstein-Barr virus (EBV). Many characteristics of the NALL-1 line were distinct from those of numerous EBV-positive lymphoblastoid cell lines previously established. The NALL-1 line grew as single-cell suspension cultures with a doubling time of 72 hours and consisted of primitive lymphoid cells with large nuclei, prominent nucleoli and scanty cytoplasm. Chromosome analysis of NALL-1 cells revealed a hypodiploid karyotype with a minute marker chromosome. NALL-1 cells are considered to have originated from the donor's leukemic cells on the basis of their cytoplasmic, morphologic and functional features. en-copyright= kn-copyright= en-aut-name=DabasakiHiroshi en-aut-sei=Dabasaki en-aut-mei=Hiroshi kn-aut-name=駄場崎浩 kn-aut-sei=駄場崎 kn-aut-mei=浩 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=財団法人積善会十全総合病院 en-keyword=急性リンパ性白血病 kn-keyword=急性リンパ性白血病 en-keyword=培養 kn-keyword=培養 en-keyword=Null 細胞株 kn-keyword=Null 細胞株 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1117 end-page=1125 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood leukocytes using sheep red blood cells　as target cells　Part II. ADCC and erythrophagocytosis of peripheral leukocytes in patients with Sjogren's syndrome or rheumatoid arthritis kn-title=ヒツジ赤血球を標的細胞とするヒト末梢白血球のAntibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 第2編 Sjogren症候群ならびに慢性関節リウマチ患者白血球のADCC活性とErythrophagocytosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent erythrophagocytosis (ADEP) of peripheral blood leukocytes in patients with Sjogren's syndrome (SjS) or rheumatoid arthritis (RA) were investigated using (51)Cr-labelled sheep red blood cells as target cells. The Lymphocytes (phagocyte depleted fraction, PDF) in healthy controls showed higher ADCC activities (58.6±9.9%) than the phagocyte-contaminated crude lymphocyte fraction (CLF) (49.1±10.9%). There was no statistically significant difference between the ADCC activities of PDF in patients with SjS (55.9±13.5%) or RA (58.8±9.6%) and those in healthy controls, although decreased ADCC activity was observed in some patients with SjS. ADCC activities of CLF in patients with SjS (61.2±14.3%) or RA (59.3±6.9%) were significantly higher than those of CLF in healthy controls. Decreased ADEP activity of monocytes in CLF was demonstrated in patients with SjS (17.9±9.4%) compared with healthy controls (37.2±10.6%), and increased ADCC activity of CLF in patients with SjS was dependent on decreased ADEP activity of contaminating monocytes. Decreased ADEP activity of monocytes in patients with RA was also suggested by these results. It was speculated that the decreased Fc-receptor function of peripheral blood monocytes, demonstrated in these patients, had originated from aberrant cell-mediated immunity in these diseases, since these Fe-receptor functions in patients with SjS did not correeate with the following conditions: associated diseases, erythrocyte sedimentation rate, titer of serum rheumatoid factor, lymphocyte count of peripheral blood, or treatment with anti-inflammatory drugs including prednisolone. en-copyright= kn-copyright= en-aut-name=IchikawaYukinobu en-aut-sei=Ichikawa en-aut-mei=Yukinobu kn-aut-name=市川幸延 kn-aut-sei=市川 kn-aut-mei=幸延 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第二内科学教室 en-keyword=antibody-dependent cell-mediated cytotoxicity kn-keyword=antibody-dependent cell-mediated cytotoxicity en-keyword=erthrophagocytosis kn-keyword=erthrophagocytosis en-keyword=sheep red blood cells kn-keyword=sheep red blood cells en-keyword=Sjogrens syndrome kn-keyword=Sjogrens syndrome en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1101 end-page=1115 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood leukocytes using sheep red blood cells as target cells Part I. Fundamental studies with special reference to erythrophagocytosis kn-title=ヒツジ赤血球を標的細胞とするヒト末梢白血球のAntibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 第1編 Erythrophagocytosisを中心とした基礎的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral leukocytes was investigated using (51)Cr-labelled sheep red blood cells (SRBC) sensitized with rabbit anit-SRBC antibody as target cells. ADCC (lysis of target cells without erythrophagocytosis) was mediated by the lymphocytes. On the other hand, phagocytes including monocytes and granulocytes showed antibody-dependent erythrophagocytosis (ADEP). ADCC of the lymphocytes was augumented by the enrichment of Fc-receptor bearing or Nylon-wool column passing cells. Phagocytes showed rapid ADEP, and granulocytes released (51)Cr-radioactivity originating from target cells into the culture medium in a short time, whereas the monocytes released little radioactivity during the incubation period. The monocytes inhibited ADCC of the lymphocytes competitivelly through ADEP, since decreased ADCC activity of monocyte-contaminated crude lymphocyte fraction was recovered by elimination of contaminating monocytes. Antibody-independent erythrophagocytosis of SRBC was mediated by monocytes, but not by granulocytes. Both ADCC of lymphocytes and ADEP of phagocytes were dependent on IgG-antibody, IgM-antibody did not participate in these phenomena. ADEP of monocytes was detected at 10(-2)-10(-6) dilution of IgG-antibody fraction (original hemmagglutination titer; × 1600), and ADCC of lymphocytes and ADEP of granulocytes were detected at 10(-2)-10(-4) and 10(-2)-10(-3) dilution of this antibody fraction, respectively. Soluble cytotoxic factors were not released from lymphocytes, monocytes or granulocytes in this assay system, because no cytotoxic activity on (51)Cr-labelled human erythrocytes used as an adjacent cell was demonstrated by any leukocyte fraction in the presence of antibody-coated SRBC. en-copyright= kn-copyright= en-aut-name=IchikawaYukinobu en-aut-sei=Ichikawa en-aut-mei=Yukinobu kn-aut-name=市川幸延 kn-aut-sei=市川 kn-aut-mei=幸延 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=antibody-dependent cell-mediated kn-keyword=antibody-dependent cell-mediated en-keyword=cytotoxicity kn-keyword=cytotoxicity en-keyword=erythrophagocytosis kn-keyword=erythrophagocytosis en-keyword=sheep red blood cells kn-keyword=sheep red blood cells END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=7-8 article-no= start-page=905 end-page=927 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Relationship between thalamic and scalp alpha rhythms in man -with cross-spectral analysis- kn-title=ヒトの視床脳波と頭皮上脳波のαリズムの相関に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Thalamic and acalp EEGs were recorded simultaneously during stereotaxic surgery. Thalamic EEGs were recorded from the ventro-lateral (VL) nucleus of the thalamus in 19 patients with parkinsonism, and from the centre median (CM) nucleus in 3 with intractable pain. Scalp EEGs following thalamotomy were also recorded. These EEGs were analyzed quantitatively by means of cross-spectral analysis. The relationships between thalamic and scalp alpha rhythms and postoperative changes of scalp alpha rhythms were studied to investigate the hypothesis that the cortical alpha rhythms are paced by thalamic generators. The results were as follows: 1) The alpha rhythms in the thalamic VL and CM nuclei had the same frequency components as those in the scalp, and were more regular and predominant than those in the scalp. 2) Significant coherences were found between alpha rhythms in the thalamic VL and CM nuclei and those in the bilateral frontal, central and parietal scalp regions. The stronger coherences were found between VL thalamic alpha rhythms and frontal and central ones, and between CM thalamic alpha rhythms and central and parietal ones. 3) Among these alpha rhythms, there were found constant phase relationships that the alpha rhythms from the VL or CM thalamic nucleus were followed, in the order of time lag, by the scalp ones in the frontal, central and then parietal regions without interhemispheric phase lag. Interhemispheric time lag was not found. 4) The alpha-coherences between the contralateral central scalp region and the VL or CM nucleus were apt to be stronger than those between the two hemispheres.5) The alpha-coherences between the central scalp region and the CM nucleus were stronger than those between the central scalp region and the dorsomedial nucleus. 6) Intrathalamic and intrahemispheric alpha-coherences were stronger than those between thalamus and scalp. Intrathalamic alpha-coherences were the strongest and had a broad bandwidth. 7) Following VL or CM thalamotomy, alpha rhythms in the frontal, central and parietal regions slowed and became irregular in frequency, but the phase relationships in these alpha rhythms remained the same as preoperative ones which spread in anteroposterior direction. However, the coherences between the fronto-centro-parietal alpha rhythms and the occipital alpha rhythms became lessened and the phase lag between parietal and occipital alpha rhythms was prolonged. On the basis of these findings, it seems to indicate that the thalamus, with close intrathalamic and internuclear relationships, has an ability to generate more stable and regular alpha rhythms than the cortex, and that the individual thalamic nucleus has the ability to generate intrinsic alpha rhythms and to regulate the alpha rhythms arising in the cortical area which receives projections from the thalamic nucleus. It also suggests that the alpha rhythms in the fronto-centro-parietal cortex, with close relationships with VL and CM nucleus, are different to some degree in synchronization from the occipital alpha rhythms. en-copyright= kn-copyright= en-aut-name=MimuraYasunaga en-aut-sei=Mimura en-aut-mei=Yasunaga kn-aut-name=三村恭永 kn-aut-sei=三村 kn-aut-mei=恭永 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳神経外科教室 en-keyword=脳波 kn-keyword=脳波 en-keyword=視床脳波 kn-keyword=視床脳波 en-keyword=αリズム kn-keyword=αリズム en-keyword=スペクトル分析 kn-keyword=スペクトル分析 en-keyword=定位脳手術 kn-keyword=定位脳手術 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=5-6 article-no= start-page=749 end-page=761 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Morphological studies on human blood basophils in immunological reactions Part II. Scanning electron microscope observation of morphological changes in basophils challenged in vitro with anti-human immunoglobulins kn-title=免疫反応下における好塩基球の形態学的研究 第2編 抗ヒト免疫グロブリンによる好塩基球の形態学的変化の走査型電子顕微鏡観察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Morphological change of blood basophils were investigated in 3 healthy controls, 4 atopic and 4 intractable asthmatics by scanning electron microscopy after they had been exposed to anti-human IgE or anti-human IgG. Most of the basophils from healthy controls, atopic and intractable asthmatics challenged with physiological saline solution (P. S. S.) were sphericalshaped. Basophils from atopic asthmatics, or basophils from healthy controls and intractable asthmatics after passively sensitized with IgE rich serum, were pear-shaped or 'swollen' when challenged with anti-IgE. In such conditions some basophils showed surface structural changes such as hemispheric projections, pore formation or ruptured cell membrane. Basophils from intractable asthmatics tended to be pear-shaped or 'swollen' and also showed surface structural changes as mentioned above when anti-IgG was added. The results show that not only IgE but also IgG may participate in the pathogenesis of bronchial asthma, especially of intractable asthma. en-copyright= kn-copyright= en-aut-name=KobayashiMakoto en-aut-sei=Kobayashi en-aut-mei=Makoto kn-aut-name=小林誠 kn-aut-sei=小林 kn-aut-mei=誠 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室/大学院医学研究科 en-keyword=好塩基球 kn-keyword=好塩基球 en-keyword=走査電顕 kn-keyword=走査電顕 en-keyword=抗ヒト免疫グロプリン kn-keyword=抗ヒト免疫グロプリン en-keyword=アトピー型喘息 kn-keyword=アトピー型喘息 en-keyword=重症難治性喘息 kn-keyword=重症難治性喘息 END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=9-10 article-no= start-page=899 end-page=913 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19821030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies of gamma-aminobutyric acid (GABA) and its metabolite on the control mechanism of secretion of anterior pituitary hormones. Part U: Effects of gamma-hydroxybutyric acid (GHB) on secretion of anterior pituitary hormones in human subjects. kn-title=Gamma-aminobutyric acid (GABA) 及びその代謝産物の下垂体前葉ホルモン分泌調節における役割に関する研究 第二報 Gamma-hydroxybutyric acid (GHB) のヒト下垂体前葉ホルモン分泌に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Gamma-hydroxybutyric acid (GHB), a physiological metabolite of gamma-aminobutyric acid (GABA) was administered to normal persons and patients with hypothalamo-pituitary disorders to examine the effect of GHB on pituitary hormone secretion. GHB stimulated the releases of growth hormone (GH), prolactin (PRL) and cortisol but not luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyrotropin (TSH) in healthy male volunteers. In patients with hypothalamic disorders, plasma GH, PRL and cortisol levels did not increase after GHB administration. In patients with diabetes insipidus, plasma GH responses to GHB were lower than those in normal control, but plasma cortisol and PRL responses were normal. In patients with hypopituitarism, plasma GH and cortisol levels did not elevate after GHB injection, but plasma PRL responses to GHB was normal in three out of patients. In patients with pituitary dwarfism, plasma PRL and cortisol levels increased after GHB injection but plasma GH did not respond to GHB. In patients with acromegaly, plasma GH responses after GHB administration was not consistent. In cases with Cushing's syndrome, plasma cortisol levels increased in patients with adrenocortical hyperplasia but not in those with adrenocortical adenoma. Although plasma GH responses after GHB injection disappeared in patients with Cushing's syndrome, plasma PRL responses was slightly greater than that in normal subjects. In a patient with Addison's disease, plasma ACTH level increased, and plasma PRL and GH responses were normal after GHB injection. To study the relationship between GHB and biogenic amines induced release of human pituitary hormones, the dopaminergic mechanism was blocked by pimozide or stimulated by CB-154 administration, and the serotonergic mechanism was blocked by parachlorophenyl-alanine (PCPA) before GHB administration. GHB induced GH release after treatment with pimozide or PCPA was not significantly different from GH release induced by GHB alone. CB-154 inhibited the increase of plasma GH induced by GHB. Plasma PRL responses to GHB was completely inhibited by CB-154 pretreatment, but not altered by pimozide or PCPA pretreatment. Plasma cortisol responses to GHB was inhibited by pimozide or PCPA pretreatment, but CB-154 had no effect on plasma cortisol responses to GHB injection. GH and cortisol releases induced by GHB was inhibited by continuous TRH infusion. These results suggest that GHB administration is a useful method for examining pituitary hormone secretion. The site of GHB action on pituitary hormone release is probably more central than pituitary in human. Not only inhibition of dopaminergic mechansim but also another mechanism may be involved in GHB induced release of human pituitary hormones. en-copyright= kn-copyright= en-aut-name=YunokiSho en-aut-sei=Yunoki en-aut-mei=Sho kn-aut-name=柚木昌 kn-aut-sei=柚木 kn-aut-mei=昌 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第三内科 en-keyword=GHB kn-keyword=GHB en-keyword=ヒト kn-keyword=ヒト en-keyword=ＧＨ kn-keyword=ＧＨ en-keyword=ＰＲＬ kn-keyword=ＰＲＬ en-keyword=Ｃｏｒｔｉｓｏｌ kn-keyword=Ｃｏｒｔｉｓｏｌ en-keyword=ＬＨ kn-keyword=ＬＨ en-keyword=Pimozide kn-keyword=Pimozide en-keyword=PCPA kn-keyword=PCPA en-keyword=CB-154 kn-keyword=CB-154 en-keyword=TRH kn-keyword=TRH END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=5-6 article-no= start-page=685 end-page=695 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on specific anti-macrophage serum (AMS) II) Distribution of macrophages in the site of pathology kn-title=特異的抗マクロファージ血清に関する研究 第二編 抗ヒト単球血清の作製と各種免疫病の病変局所におけるマクロファージの分布 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A cultured human monocytic leukemia cell line, J-III, was used to make heterologous antiserum against human peripheral monocyte surface antigens. To eliminate non-specific antibodies, this antiserum was extensively absorbed with human erythrocytes, neutrophils, nonglass adherent mononuclear cells (mainly lymphocytes) and fetal kidney homogenate. The absorbed serum showed high specificity against human peripheral monocytes, suggesting the presence of similar antigenic determinants on J-III cells and human monocytes. Using this purified serum, an immunofluorescent technique was used to demonstrate macrophages in the site of pathology in various immunological diseases. In synovial tissues from patients with rheumatoid arthritis, positively stained cells were observed in the center and surrounding area of the germinal center. Small numbers of positive cells had also infiltrated around small vessels. Mesangial cells of renal biopsy specimens from patients with SLE were not stained. In Sjogren's disease, salivary glands showed granulomatous change, in which small numbers of diffusely scattered positive cells were observed. Similar changes were seen in Hashimoto thyroiditis. Patients with Behcet's disease showed only a few positive cells in the site of erythema nodosum. There were a small number of infiltrated positive cells in biopsied skin at the site of a positive tuberculin reaction. en-copyright= kn-copyright= en-aut-name=MiyakeSusumu en-aut-sei=Miyake en-aut-mei=Susumu kn-aut-name=三宅晋 kn-aut-sei=三宅 kn-aut-mei=晋 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第三内科教室 en-keyword=特異的抗ヒト単球血清の作製 kn-keyword=特異的抗ヒト単球血清の作製 en-keyword=多臓器吸着法 kn-keyword=多臓器吸着法 en-keyword=J-111細胞 kn-keyword=J-111細胞 en-keyword=マクロファージの同定 kn-keyword=マクロファージの同定 en-keyword=各種免疫病 kn-keyword=各種免疫病 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=3-4 article-no= start-page=511 end-page=518 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19790430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The relationship between incurable gastric ulcer and Candida albicans at the ulcer base I. Immuno-fluorescent study of human gastric ulcer using anti-Candida albicans antisera kn-title=難治性胃潰瘍とCandida albicansとの関り合いに関する研究 第I編 螢光抗体法によるヒト胃潰瘍組織部Candida albicansの免疫組織学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Forty-seven cases of gastric ulcer including some with ulcerated carcinoma were investigated by an immuno-fluorescent technique using anti-Candida albicans antisera. Fresh tissue containing ulcer base was also stained with PAS reagent. Candida albicans at the ulcer base was positive for fluorescence. The cytoplasm of many histiocytes was also fluorescent. Histiocytes were predominant at the ulcer base and were present even in the muscular and subserosal layers. The cytoplasm of these cells was PAS-positive. These data suggest that Candida albicans at the necrotic ulcer base is not a simple opportunistic growth, but that some of its watersoluble factor may be involved in the poor curability of gastric ulcer. en-copyright= kn-copyright= en-aut-name=HitomiFumio en-aut-sei=Hitomi en-aut-mei=Fumio kn-aut-name=人見文雄 kn-aut-sei=人見 kn-aut-mei=文雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科学教室 en-keyword=難治性潰瘍 kn-keyword=難治性潰瘍 en-keyword=Candiba albicans kn-keyword=Candiba albicans en-keyword=螢光抗体法 kn-keyword=螢光抗体法 en-keyword=可溶性抗原 kn-keyword=可溶性抗原 en-keyword=組織球 kn-keyword=組織球 END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=7-8 article-no= start-page=683 end-page=692 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the diagnosis and treatment of human lung cancer using double-layered soft agar cloning assay U. Direct cloning of human lung cancer cells and in vitro chemosensitivity test kn-title=コロニー形成法を利用したヒト肺癌の診断と治療に関する研究 第2編 ヒト肺癌細胞のdirect cloning及びin vitro制癌剤感受性試験 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The extent of tumor dissemination at the time of diagnosis is an important predictor for survival of lung cancer patients. In vitro sensitivity to drugs is helpful for successful chemotherapy of advanced lung cancer patients. A major objective of the present study was to clarify the significance of direct cloning assay for the detection of metastatic sites as well as for the selection of sensitive drugs for individual patients. Tumor cell colony growth was evaluated in an enriched double-layered soft agar system. Colonies were successfully grown from 5 of 7 cytology positive effusions, and 4 of 5 histology/cytology positive bone marrow aspirates. Two of 17 histology/cytology negative bone marrow aspirates yielded colonies, whereas 5 cytology negative effusions yielded no colonies. Colony growth was observed in 3 of 5 tumor specimens obtained surgically. For in vitro chemosensitivity, tumor cells were exposed for 1 hour prior to plating. Although colonies were successfully grown from specimens of 5 patients, none of them were sensitive to the drugs tested. Three of the 5 patients were treated with intensive combination chemotherapy including therapy with drugs which had been defined to be resistant in the in vitro chemosensitivity test and no response was observed in these cases. In this sense, in vitro sensitivity correlated with in vivo sensitivity. en-copyright= kn-copyright= en-aut-name=MiyaiMasahiro en-aut-sei=Miyai en-aut-mei=Masahiro kn-aut-name=宮井正博 kn-aut-sei=宮井 kn-aut-mei=正博 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=tumor stem cell assay in man kn-keyword=tumor stem cell assay in man en-keyword=lung cencer kn-keyword=lung cencer en-keyword=in vitro chemosensitivity test kn-keyword=in vitro chemosensitivity test END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=7-8 article-no= start-page=673 end-page=681 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the diagnosis and treatment of human lung cancer using double-layered soft agar cloning assay I. In vitro chemosensitivity test on permanent cell lines from adeno-, epidermoid and small cell carcinoma of the lung using double-layered soft agar cloning assay kn-title=コロニー形成法を利用したヒト肺癌の診断ならびに治療に関する研究 第1編 ヒト肺癌細胞株(腺癌,扁平上皮癌,小細胞癌)のin vitro制癌剤感受性試験 en-subtitle= kn-subtitle= en-abstract= kn-abstract=An in vitro chemosensitivity test on permanent cell lines from adeno-, epidermoid and small cell carcinoma of the lung was performed using a double-layered soft agar cloning assay. Drugs tested in the present study were Adriamycin, 40497S, Mitomycin C, cis-Dichlorodiammineplatinum, Methotrexate and Vincristine. Adriamycin, 40497S, Mitomycin C and cis-Dichlorodiammineplatinum showed dose-dependent cytotoxity. Methotrexate and Vincristine did not show a significant cytotoxity when the cell lines were exposed for one hour. Differences in chemosensitivity to the six drugs examined among the three cell lines was relatively small e.g. the lethal dose 90% (LD90) of Adriamycin was 0.19mcg/ml in adenocarcinoma, 0.46mcg/ml in epidermoid carcinoma and 0.27mcg/ml in small cell carcinoma. The lack of a difference in chemosensitivity in vitro might be attributed to the fact that the cell lines had been established from bronchogenic carcinomas which were refractory to intensive combination chemotherapy. en-copyright= kn-copyright= en-aut-name=MiyaiMasahiro en-aut-sei=Miyai en-aut-mei=Masahiro kn-aut-name=宮井正博 kn-aut-sei=宮井 kn-aut-mei=正博 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=human lung cancer cell lines kn-keyword=human lung cancer cell lines en-keyword=in vitro chemosensitivity test kn-keyword=in vitro chemosensitivity test en-keyword=cloning assay kn-keyword=cloning assay END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=5-6 article-no= start-page=471 end-page=485 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Analgestic effect of histamine kn-title=ヒスタミン脳内投与による鎮痛作用発現について en-subtitle= kn-subtitle= en-abstract=All the test drugs were dissolved in saline and a fixed amount of 0.5μl was injected into the lateral ventricle of conscious mice (dd-Y, male). Injection of the same amount of saline exhibited a negligible effect. After mice were injected intraperitoneally with 0.1ml/10g of 0.02% aqueous solution of phenylquinone or 0.6% acetic acid solution, the number of writhing syndrome was counted for 30min. When histamine (Hi) was given immediately after intraperitoneal injections of those chemical stimulants, Hi displayed a protective effect and the number of writhes decreased significantly at doses higher than 5μg/body dose-dependently. Electrical stimulation (25V, 1Hz, 25msec) to the mouse tail evokes a squeak response with the mean reaction time of 5.6±0.4 sec. When Hi was applied, the reaction time was prolonged significantly at the doses higher than 10μg/body in a dose-dependent fashion. A similar analgesic effect of Hi was also displayed in a hot plate method which was carried out at 55℃. In this case, application of 10μg or more of Hi definitely prolonged the pain reaction time. When morphine was given subcutaneously at the dose of 3mg/kg which is less than the minimum effective dose, morphine augmented the analgesic effect of Hi. In accordance with this, pretreatment of naloxone (0.01mg/kg) antagonized the analgesic action of Hi almost completely. Further, by injecting the subthreshold dose of leucine-enkephalin (5mg/kg) prior to Hi administration, analgesic effect of the latter was enhanced. When the H(1)-receptor blocking agent, diphenhydramine was injected simultaneously with Hi either intraventricularly or intravenously at the doses less than affecting the reaction time in a hot plate method, it antagonized the analgesic action of Hi. However, the administration of H(2)-receptor blocking agent, cimetidine was not effective in antagonizing the Hi action. From these findings, it was concluded that Hi injected into the ventricle reveals some analgesic actions which appear via H(1) receptor and the mechanism of Hi analgesia may be related in some way to the enkephalinergic mechanism. kn-abstract=中枢神経系に関するcatecholamine(CA)や5-hydroxytryptamine(5-HT)の生化学的及び薬理学的研究に比較すると,Histamine(Hi)の中枢作用は最近急速に注目を浴びてはいるが,まだ充分には理解されていない.しかし,哺乳動物の中枢神経系内におけるHiの分布は,CAや5-HTと極めて類似し,特に下垂体や視床下部には高濃度に分布する事が知られており(1)),Hiも中枢神経系においてneurotransmitterまたはneuromodulatorとしての役割を果している可能性は高いと考えられている(2)).Feldbergら(3))は,無麻酔ネコの側脳室にHi 100μg/bodyを注入した際,ネコの活動性が低下し,傾眠や筋力低下が観察される事を報告している.最近,Nowakら(4))はHiをラット脳室内に投与した際,運動量の低下やcatalepsyの発現がみられる事を報告している.また,ラット脳室内へのHi投与後に,動脈血圧が上昇する事も知られている(5)).マウスの場合には,Hiの脳内投与後体温下降が誘発される事が報告されている(6)).このようにHiの脳室内投与が,動物の行動,体温,血圧等に影響を与える事は知られているが,Hiが鎮痛効果を誘発し得るか否かについて検討された報告は見当らない.Hiをヒトに静注すると,搏動性の頭痛が誘発され(7)),皮内注射では局所の疼痛を惹起する事(8))が知られている.しかし, Sanuki(9))は,マウスにHiの大量を皮下注射したところ,鎮痛効果がみられたと報告している.Hiをマウスの末梢側に非経口投与した際発現する種々な中枢作用には,脳内にとりこまれたHiが関与している可能性が報告されている(10,11))ので,Sanukiの実験でも末梢側に投与された大量のHiの一部が中枢神経系に移行し,鎮痛効果を発現している可能性は否定できない.著者はマウス脳内にHiを投与した際,鎮痛効果と可成り密接に相関すると考えられる仮性疼痛反応に対するHiの効果を調べると共に,morphineやその関連物質の前処置がHiの効果をいかに修飾するのかを調べ,Hi鎮痛効果発現の機序についても検討した.また,Hiの鎮痛効果がH(1)またはH(2)-receptorのいずれとより密接に相関するのかについての検討も併せて行なった. en-copyright= kn-copyright= en-aut-name=OkadaIkuo en-aut-sei=Okada en-aut-mei=Ikuo kn-aut-name=岡田郁男 kn-aut-sei=岡田 kn-aut-mei=郁男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学歯学部口腔外科学教室 en-keyword=ヒスタミン kn-keyword=ヒスタミン en-keyword=鎮痛作用 kn-keyword=鎮痛作用 en-keyword=脳内投与 kn-keyword=脳内投与 en-keyword=モルヒネ kn-keyword=モルヒネ en-keyword=抗ヒスタミン剤 kn-keyword=抗ヒスタミン剤 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒト病理組織における myeloid DC と plasmacytoid DC の免疫組織化学的識別 kn-title=Immunohistochemical discrimination of plasmacytoid dendritic cells from myeloid dendritic cells in human pathological tissues en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NishikawaYuriko en-aut-sei=Nishikawa en-aut-mei=Yuriko kn-aut-name=西川由利子 kn-aut-sei=西川 kn-aut-mei=由利子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END