start-ver=1.4
cd-journal=joma
no-vol=525
cd-vols=
no-issue=7
article-no=
start-page=1586
end-page=1598
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170501
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Several regions of the brain and spinal cord control male reproductive function. We previously demonstrated that the gastrin-releasing peptide (GRP) system, located in the lumbosacral spinal cord of rats, controls spinal centers to promote penile reflexes during male copulatory behavior. However, little information exists on the male-specific spinal GRP system in animals other than rats. The objective of this study was to examine the functional generality of the spinal GRP system in mammals using the Asian house musk shrew (Suncus murinus; suncus named as the laboratory strain), a specialized placental mammal model. Mice are also used for a representative model of small laboratory animals. We first isolated complementary DNA encoding GRP in suncus. Phylogenetic analysis revealed that suncus preproGRP was clustered to an independent branch. Reverse transcription-PCR showed that GRP and its receptor mRNAs were both expressed in the lumbar spinal cord of suncus and mice. Immunohistochemistry for GRP demonstrated that the sexually dimorphic GRP system and male-specific expression/distribution patterns of GRP in the lumbosacral spinal cord in suncus are similar to those of mice. In suncus, we further found that most GRP-expressing neurons in males also express androgen receptors, suggesting that this male-dominant system in suncus is also androgen-dependent. Taken together, these results indicate that the sexually dimorphic spinal GRP system exists not only in mice but also in suncus, suggesting that this system is a conserved property in mammals.
en-copyright=
kn-copyright=

en-aut-name=TamuraKei
en-aut-sei=Tamura
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KobayashiYasuhisa
en-aut-sei=Kobayashi
en-aut-mei=Yasuhisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HirookaAsuka
en-aut-sei=Hirooka
en-aut-mei=Asuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakanamiKeiko
en-aut-sei=Takanami
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OtiTakumi
en-aut-sei=Oti
en-aut-mei=Takumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=JogaharaTakamichi
en-aut-sei=Jogahara
en-aut-mei=Takamichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OdaSen-ichi
en-aut-sei=Oda
en-aut-mei=Sen-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=SakamotoTatsuya
en-aut-sei=Sakamoto
en-aut-mei=Tatsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=SakamotoHirotaka
en-aut-sei=Sakamoto
en-aut-mei=Hirotaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=5
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=6
en-affil= Laboratory of Animal Management and Resources, Department of Zoology, Okayama University of Science
kn-affil=

affil-num=7
en-affil= Laboratory of Animal Management and Resources, Department of Zoology, Okayama University of Science
kn-affil=

affil-num=8
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=9
en-affil=Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=RRID
kn-keyword=RRID
en-keyword=AB_2060157
kn-keyword=AB_2060157
en-keyword=RRID: AB_2571636
kn-keyword=RRID: AB_2571636
en-keyword=RRID: AB_626757
kn-keyword=RRID: AB_626757
en-keyword=Suncus murinus (suncus)
kn-keyword=Suncus murinus (suncus)
en-keyword=gastrin-releasing peptide
kn-keyword=gastrin-releasing peptide
en-keyword=male reproductive function
kn-keyword=male reproductive function
en-keyword=sexual dimorphism
kn-keyword=sexual dimorphism
en-keyword=spinal cord
kn-keyword=spinal cord
END