start-ver=1.4 cd-journal=joma no-vol=34 cd-vols= no-issue=16 article-no= start-page=2316 end-page=2322 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=201308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Discrimination of glycoproteins via two-color laser-induced fluorescence detection coupled with postcolumn derivatization in capillary electrophoresis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Here, we report a novel method consisting of capillary electrophoretic separation followed by two-color LIF detection with postcolumn derivatization. The method can be used to discriminate glycoproteins in a protein mixture containing both glycosylated and unglycosylated proteins. The detector permitted simultaneous measurements of two electropherograms obtained by 450 nm (diode laser) and 532 nm (Nd:YAG laser) lasers excited native proteins following postcolumn derivatization with naphthalene-2,3-dicarboxaldehyde and concanavalin A (Con A) labeled with tetramethylrhodamine (rhodamine-labeled Con A), respectively. So, a protein can be assigned as glycosylated if it shows a peak at the same migration time in both electropherograms. According to the proposed principle, in a single run we discriminated a glycosylated protein (thyroglobulin) from an unglycosylated protein (albumin) in the presence of rhodamine-labeled Con A. Because the methodology permits the simultaneous detection of native proteins and their complexes with a fluorescently labeled probe, it should have broad applicability to binding assays. en-copyright= kn-copyright= en-aut-name=TabaraAyumi en-aut-sei=Tabara en-aut-mei=Ayumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=Capillary electrophoresis kn-keyword=Capillary electrophoresis en-keyword=Concanavalin A kn-keyword=Concanavalin A en-keyword=Glycoprotein kn-keyword=Glycoprotein en-keyword=Postcolumn derivatization kn-keyword=Postcolumn derivatization en-keyword=Two-color laser-induced fluorescence kn-keyword=Two-color laser-induced fluorescence END