start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=10
article-no=
start-page=1051
end-page=1058
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160510
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Regulation of Vibrio mimicus metalloprotease (VMP) production by the quorum-sensing master regulatory protein, LuxR
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=   Vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. This pathogen secretes a relatively important toxin named V. mimicus metalloprotease (VMP). In this study, we clarified regulation of the VMP production according to the quorum-sensing master regulatory protein named LuxR. First, the full length of luxR gene, encoding LuxR, was detected in V. mimicus strain E-37, an environmental isolate. Next, the putative consensus binding sequence of LuxR protein could be detected in the upstream (promoter) region of VMP encoding gene, vmp. Finally, the effect of disruption of luxR gene on the expression of vmp and production of VMP was evaluated. Namely, the expression of vmp was significantly diminished by luxR disruption and the production of VMP was severely altered. Taken together, here we report that VMP production is under the positive regulation of the quorum-sensing master regulatory protein, LuxR.
en-copyright=
kn-copyright=

en-aut-name=Abdel]SattarEl]Shaymaa
en-aut-sei=Abdel]Sattar
en-aut-mei=El]Shaymaa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Miyoshi Shin]ichi
en-aut-sei=Miyoshi
en-aut-mei= Shin]ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=ElgamlAbdelaziz
en-aut-sei=Elgaml
en-aut-mei=Abdelaziz
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=3
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=LuxR protein
kn-keyword=LuxR protein
en-keyword=Metalloprotease
kn-keyword=Metalloprotease
en-keyword=Quorum-sensing
kn-keyword=Quorum-sensing
en-keyword=Vibrio mimicus
kn-keyword=Vibrio mimicus
END