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ID 52861
FullText URL
Author
Inoue, Kentaro
Teshigawara, Sanae Kaken ID
Murakami, Kazutoshi ORCID Kaken ID publons
Ogawa, Daisuke Kaken ID
Terami, Takahiro
Katayama, Akihiro
Tone, Atsuhito
Iseda, Izumi
Hida, Kazuyuki
Yamada, Masao
Ogawa, Tomohisa
Abstract
We analyzed the urine samples of patients with type 2 diabetes at various stages of diabetic nephropathy by lectin microarray to identify a biomarker to predict the progression of diabetic nephropathy. Japanese patients with type 2 diabetes at various stages of nephropathy were enrolled and we performed lectin microarray analyses (n = 17) and measured urinary excretion of fetuin-A (n = 85). The increased signals of urine samples were observed in Sia alpha 2-6Gal/GalNAc-binding lectins (SNA, SSA, TJA-I) during the progression of diabetic nephropathy. We next isolated sialylated glycoproteins by using SSA-lectin affinity chromatography and identified fetuin-A by liquid chromatography-tandem mass spectrometer. Urinary excretion of fetuin-A significantly increased during the progression of albuminuria (A1, 0.40 +/- 0.43; A2, 0.60 +/- 0.53; A3 1.57 +/- 1.13 ng/gCr; p = 7.29x10(-8)) and of GFR stages (G1, 0.39 +/- 0.39; G2, 0.49 +/- 0.45; G3, 1.25 +/- 1.18; G4, 1.34 +/- 0.80 ng/gCr; p = 3.89x10(-4)). Multivariate logistic regression analysis was employed to assess fetuin-A as a risk for diabetic nephropathy with microalbuminuria or GFR<60 mL/min. Fetuin-A is demonstrated as a risk factor for both microalbuminuria and reduction of GFR in diabetic nephropathy with the odds ratio of 4.721 (1.881-11.844) and 3.739 (1.785-7.841), respectively. Collectively, the glycan profiling analysis is useful method to identify the urine biomarkers and fetuin-A is a candidate to predict the progression of diabetic nephropathy.
Published Date
2013-10-15
Publication Title
PLoS ONE
Volume
volume8
Issue
issue10
Publisher
Public Library Science
ISSN
1932-6203
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52832
language
English
Copyright Holders
© 2013 Inoue et al.
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publisher
Refereed
True
DOI
Web of Science KeyUT