start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=12 article-no= start-page=1334 end-page=1341 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=201412 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Assessment of intraoral mucosal pain induced by the application of capsaicin en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 μg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 μg doses of capsaicin were significantly greater than that by the 1 μg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury. en-copyright= kn-copyright= en-aut-name=TerayamaRyuji en-aut-sei=Terayama en-aut-mei=Ryuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaruhamaKotaro en-aut-sei=Maruhama en-aut-mei=Kotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsuchiyaHiroki en-aut-sei=Tsuchiya en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MizutaniMasahide en-aut-sei=Mizutani en-aut-mei=Masahide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IidaSeiji en-aut-sei=Iida en-aut-mei=Seiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SugimotoTomosada en-aut-sei=Sugimoto en-aut-mei=Tomosada kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Capsaicin kn-keyword=Capsaicin en-keyword=Behaviour kn-keyword=Behaviour en-keyword=c-Fos kn-keyword=c-Fos en-keyword=Medullary dorsal horn kn-keyword=Medullary dorsal horn en-keyword=Immunohistochemistry kn-keyword=Immunohistochemistry en-keyword=Nerve injury kn-keyword=Nerve injury END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=133 end-page=141 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=201402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objectives: The present study aimed to identify the effect of memory-related genes on male rats tested for spatial memory with either molar teeth extraction or its restoration by occlusal support using experimental dentures. Design: Memory-related genes were detected from hippocampi of male Wistar rats (exposed to teeth extraction with or without dentures, or no extraction (control)) (7-week old) after behavioural testing (via the radial maze task) using a DNA microarray. The time course of the expression of these genes was evaluated by quantitative real-time polymerase chain reaction (PCR) (on 49-week-old rats). Results: In preliminary experiments, to determine which memory genes are affected by spatial memory training, DNA microarray analysis revealed that thyrotropin-releasing hormone (Trh) and tenascin XA (Tnxa) were up-regulated and neuronatin (Nnat) and S100a9 were down-regulated after the maze training. The expression of Tnxa, Nnat and S100a9 of 49-week-old rats (during the time course) via quantitative real-time PCR was consistent with the results of microarrays of the preliminary experiment. Expression of Trh that was evaluated by quantitative real-time PCR did not agree with the results for this gene from the microarray for all groups. Therefore, expression of Trh may have increased in only young, trained rats. The expression of S100a9 prior to the maze task was down-regulated in only the extraction group. Conclusion: These results demonstrated that Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. This study also indicated that S100a9 is a memory-related gene, which is affected by the presence of occlusal support. en-copyright= kn-copyright= en-aut-name=IidaSachiyo en-aut-sei=Iida en-aut-mei=Sachiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HaraTetsuya en-aut-sei=Hara en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiDaisuke en-aut-sei=Araki en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Ishimine-KurodaChisa en-aut-sei=Ishimine-Kuroda en-aut-mei=Chisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KurozumiAkimasa en-aut-sei=Kurozumi en-aut-mei=Akimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SakamotoShunichi en-aut-sei=Sakamoto en-aut-mei=Shunichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MiyazakiTakako en-aut-sei=Miyazaki en-aut-mei=Takako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MinagiShogo en-aut-sei=Minagi en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=2 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=3 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=4 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=5 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=6 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=7 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil affil-num=8 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Occlusal & Oral Funct Rehabil en-keyword=Gene expression kn-keyword=Gene expression en-keyword=Memory and learning kn-keyword=Memory and learning en-keyword=Hippocampus kn-keyword=Hippocampus en-keyword=Trh kn-keyword=Trh en-keyword=Tnxa kn-keyword=Tnxa en-keyword=Nnat kn-keyword=Nnat en-keyword=S100a9 kn-keyword=S100a9 END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=2 article-no= start-page=306 end-page=308 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100113 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Palladium- and base-free synthesis of conjugated ynones by cross-coupling reactions of alkynylboronates with acid chlorides mediated by CuCl en-subtitle= kn-subtitle= en-abstract= kn-abstract=Alkynylboronates can be employed as a practical and versatile precursor for a variety of pi-conjugated organic compounds. In the presence of Cu(I) salt, cross-coupling reactions of acid chlorides with alkynylboronates giving rise to the corresponding conjugated ynones takes place readily in aprotic polar solvents such as DMI under neutral conditions. en-copyright= kn-copyright= en-aut-name=NishiharaYasushi en-aut-sei=Nishihara en-aut-mei=Yasushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SaitoDaisuke en-aut-sei=Saito en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoueEiji en-aut-sei=Inoue en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkadaYoshiaki en-aut-sei=Okada en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyazakiMikihiro en-aut-sei=Miyazaki en-aut-mei=Mikihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InoueYoshiaki en-aut-sei=Inoue en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakagiKentaro en-aut-sei=Takagi en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=5 article-no= start-page=907 end-page=913 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201107 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Long-term exposure to methylmercury and psychiatric symptoms in residents of Minamata, Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=Introduction: It is well-known that prenatal or postnatal exposure to methylmercury can produce neurological signs in adults and children, exemplified by a case of large-scale poisoning in Minamata, Japan, in the 1950s. However, evidence regarding whether pre- or postnatal exposure to methylmercury causes psychiatric symptoms (e.g., impairment of intelligence and mood and behavioral dysfunction) is still limited-excluding cases of fetal Minamata disease patients. Methods: We evaluated the effects of pre- or postnatal exposure to methylmercury on psychiatric symptoms using data derived from a 1971 population-based survey in Minamata and neighboring communities. We adopted residential areas as an exposure indicator and psychiatric symptoms as the outcome. Then, we estimated the adjusted prevalence odds ratio (POR) and confidence interval (Cl) of psychiatric symptoms in relation to residential area. Results: There were 904 participants in Minamata (high exposure area), 1700 in Goshonoura (middle exposure area), and 913 in Ariake (low exposure area). Compared to the Ariake area, participants in the Minamata area manifested psychiatric symptoms more frequently: PORs for impairment of intelligence and mood and behavioral dysfunction were 5.2 (95% Cl: 3.7-7.3) and 4.4 (95% Cl: 2.9-6.7), respectively. Furthermore, participants with psychiatric symptoms in the Minamata area more frequently had neurological signs. Peaks in prevalence of psychiatric symptoms occurred around age 20 and in older age adults in the area. These findings did not change when we excluded those who had been officially certified as Minamata disease patients by that time. Conclusions: The present study suggests a relationship between pre- or postnatal exposure to methylmercury and psychiatric symptoms among the general population in Minamata even after excluding officially certified patients. en-copyright= kn-copyright= en-aut-name=YorifujiTakashi en-aut-sei=Yorifuji en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsudaToshihide en-aut-sei=Tsuda en-aut-mei=Toshihide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoueSachiko en-aut-sei=Inoue en-aut-mei=Sachiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HaradaMasazumi en-aut-sei=Harada en-aut-mei=Masazumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Environmental Epidemiology, Okayama University Graduate School of Environmental Science affil-num=3 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Environmental pollution kn-keyword=Environmental pollution en-keyword=Epidemiological studies kn-keyword=Epidemiological studies en-keyword=Methylmercury compounds kn-keyword=Methylmercury compounds en-keyword=Minamata disease kn-keyword=Minamata disease en-keyword=Prenatal exposure delayed effects kn-keyword=Prenatal exposure delayed effects en-keyword=Psychiatric disorders kn-keyword=Psychiatric disorders END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=10 article-no= start-page=3388 end-page=3391 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Superhydrophilicity on nano-rough carbon surfaces achieved by hyperthermal oxygen-atom beam exposure en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to investigate a method to increase hydrophilicity on nano-rough carbon surfaces, a nano-rough surface of C(60) film and an atomically flat surface of highly oriented pyrolytic graphite (HOPG) were oxidized by hyperthermal oxygen-atom beam exposure and the hydrophilicities of the surfaces were investigated. Superhydrophilicity were achieved on these exposed carbon surfaces, which had low O/C ratio of approximately 28% and surface roughness (Ra) of approximately 3 nm. The direct oxidations on sp(2) bonded carbon atoms (basal plane) of these two carbon materials by the exposure of hyperthermal O-atom beam would contribute the superhydrophilicity. en-copyright= kn-copyright= en-aut-name=KinoshitaHiroshi en-aut-sei=Kinoshita en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakayamaTakashi en-aut-sei=Nakayama en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsumotoNaohiro en-aut-sei=Matsumoto en-aut-mei=Naohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhmaeNobuo en-aut-sei=Ohmae en-aut-mei=Nobuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Mechanical Engineering, Faculty of Engineering, Okayama University affil-num=2 en-affil= kn-affil=Department of Mechanical Engineering, Faculty of Engineering, Okayama University affil-num=3 en-affil= kn-affil=Department of Mechanical Engineering, Faculty of Engineering, Okayama University affil-num=4 en-affil= kn-affil=Department of Mechanical Engineering, Faculty of Engineering, Okayama University END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue=19 article-no= start-page=5901 end-page=5904 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20111001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=In vivo anti-inflammatory and antioxidant properties of ellagitannin metabolite urolithin A en-subtitle= kn-subtitle= en-abstract= kn-abstract=Urolithin A is a major metabolite produced by rats and humans after consumption of pomegranate juice or pure ellagitannin geraniin. In this study, we investigated the anti-inflammatory effect of urolithin A on carrageenan-induced paw edema in mice. The volume of paw edema was reduced at 1 h after oral administration of urolithin A. In addition, plasma in treated mice exhibited significant oxygen radical antioxidant capacity (ORAC) scores with high plasma levels of the unconjugated form at 1 h after oral administration of urolithin A. These results indicate strong associations among plasma urolithin A levels, the plasma ORAC scores, and anti-inflammatory effects and may help explain a mechanism by which ellagitannins confer protection against inflammatory diseases. en-copyright= kn-copyright= en-aut-name=IshimotoHidekazu en-aut-sei=Ishimoto en-aut-mei=Hidekazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShibataMari en-aut-sei=Shibata en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MyojinYuki en-aut-sei=Myojin en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ItoHideyuki en-aut-sei=Ito en-aut-mei=Hideyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SugimotoYukio en-aut-sei=Sugimoto en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TaiAkihiro en-aut-sei=Tai en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HatanoTsutomu en-aut-sei=Hatano en-aut-mei=Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Pharmacognosy, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Pharmacology, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Department of Pharmacognosy, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Pharmacology, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima affil-num=7 en-affil= kn-affil=Department of Pharmacognosy, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Ellagitannin kn-keyword=Ellagitannin en-keyword=Urolithin A kn-keyword=Urolithin A en-keyword=Antioxidant activity kn-keyword=Antioxidant activity en-keyword=Anti-inflammatory activity kn-keyword=Anti-inflammatory activity END start-ver=1.4 cd-journal=joma no-vol=66 cd-vols= no-issue=51 article-no= start-page=9659 end-page=9666 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20101218 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of pyrrolidine-based oxy-peptide nucleic acids carrying four types of nucleobases and their transport into cytoplasm en-subtitle= kn-subtitle= en-abstract= kn-abstract=We synthesized 16 pyrrolidine-based oxy-peptide nucleic acid (POPNA) monomers carrying four different nucleobases onto four different stereoisomers of pyrrolidine rings. Using these monomers, we prepared POPNA oligomers, which formed sequence-specific hybrids with DNAs. The oligomer configurations influenced the hybrid stability. The oligomers were not taken into CHO cells. However, they could enter the cell cytoplasm when mixed with the influenza virus hemagglutinin peptide-arginine heptamer conjugate. en-copyright= kn-copyright= en-aut-name=KitamatsuMizuki en-aut-sei=Kitamatsu en-aut-mei=Mizuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiAkiko en-aut-sei=Takahashi en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtsukiTakashi en-aut-sei=Ohtsuki en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SisidoMasahiko en-aut-sei=Sisido en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Bioscience and Biotechnology, Okayama University affil-num=2 en-affil= kn-affil=Department of Bioscience and Biotechnology, Okayama University affil-num=3 en-affil= kn-affil=Department of Bioscience and Biotechnology, Okayama University affil-num=4 en-affil= kn-affil=Department of Bioscience and Biotechnology, Okayama University en-keyword=Peptide nucleic acid kn-keyword=Peptide nucleic acid en-keyword=Solid-phase peptide synthesis kn-keyword=Solid-phase peptide synthesis en-keyword=Cell-penetrating peptide kn-keyword=Cell-penetrating peptide en-keyword=Confocal laser scanning microscopy kn-keyword=Confocal laser scanning microscopy END start-ver=1.4 cd-journal=joma no-vol=20 cd-vols= no-issue=20 article-no= start-page=5976 end-page=5978 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20101015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino acids as fluorescent tags en-subtitle= kn-subtitle= en-abstract= kn-abstract=EGF receptor-binding peptides could be found by a peptide screening method using fifteen fluorescent amino acids as fluorescent tags. Of 225 peptides, we found an 8-mer peptide containing a dipeptide unit, Y-F, which was the strongest binding peptide to the EGF receptor. en-copyright= kn-copyright= en-aut-name=KitamatsuMizuki en-aut-sei=Kitamatsu en-aut-mei=Mizuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoTakahiro en-aut-sei=Yamamoto en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FutamiMidori en-aut-sei=Futami en-aut-mei=Midori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SisidoMasahiko en-aut-sei=Sisido en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University en-keyword=Peptide kn-keyword=Peptide en-keyword=Peptide library kn-keyword=Peptide library en-keyword=Peptide screening kn-keyword=Peptide screening en-keyword=EGFR kn-keyword=EGFR en-keyword=Fluorescent amino acid kn-keyword=Fluorescent amino acid END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=1 article-no= start-page=35 end-page=40 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Occlusal disharmony induces BDNF level in rat submandibular gland en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objectives: Brain-derived neurotrophic factor (BDNF), which is produced in rat submandibular gland, is one of the most abundant neurotrophins in the central nervous system. It is generally accepted that occlusal disharmony causes stress. The purpose of the present study was to investigate whether occlusal disharmony-induced chronic stress affects BDNF levels and morphology in rat submandibular gland. Design: Eight wks old male Wistar rats (n = 21) were randomly divided into three groups of 7 rats. In a control (C) group, the rats received no treatment for 8 wks. In a molar cusp-less (OD) group, maxillary molar cusps were cut off with a dental turbine at baseline and kept for 8 wks. In a molar cusp-less + recovered cusp (OR) group, maxillary molar cusps were cut off and then were recovered after 4 wks using resin material. After the experimental period, expression of BDNF mRNA and protein as well as histological findings were evaluated in the submandibular glands. The comparisons between the groups were made using the Mann-Whitney U test with Bonferroni correction. Results: The OD group showed a significant increase in submandibular gland BDNF mRNA and protein expression after 8 wks, and plasma adrenocorticotropic hormone and corticosterone levels increased in a time-dependent manner. There were no significant differences in BDNF expression in the submandibular glands and in levels of plasma adrenocorticotropic hormone and corticosterone between the OR and C groups. Conclusions: These results indicate that psychological stress induced by occlusal disharmony reversibly induces BDNF expression in the rat submandibular gland. en-copyright= kn-copyright= en-aut-name=IrieKoichiro en-aut-sei=Irie en-aut-mei=Koichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EkuniDaisuke en-aut-sei=Ekuni en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TomofujiTakaaki en-aut-sei=Tomofuji en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AzumaTetsuji en-aut-sei=Azuma en-aut-mei=Tetsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=EndoYasumasa en-aut-sei=Endo en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KasuyamaKenta en-aut-sei=Kasuyama en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MoritaManabu en-aut-sei=Morita en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Occlusal disharmony kn-keyword=Occlusal disharmony en-keyword=Brain-derived neurotrophic factor kn-keyword=Brain-derived neurotrophic factor en-keyword=Submandibular gland kn-keyword=Submandibular gland en-keyword=Stress kn-keyword=Stress END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=5 article-no= start-page=1198 end-page=1204 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200905 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Smad3 activates the Sox9-dependent transcription on chromatin en-subtitle= kn-subtitle= en-abstract= kn-abstract=Transforming growth factor (TGF)-β has an essential role for the Sry-type high-mobility-group box (Sox)-regulated chondrogenesis. Chondrogenic differentiation is also controlled by chromatin-mediated transcription. We have previously reported that TGF-β-regulated Smad3 induces chondrogenesis through the activation of Sox9-dependent transcription. However, the cross-talk between TGF-β signal and Sox9 on chromatin-mediated transcription has not been elucidated. In the present study, we investigated the activity of Smad3, Sox9, and coactivator p300 using an in vitro chromatin assembly model. Luciferase reporter assays revealed that Smad3 stimulated the Sox9-mediated transcription in a TGF-β-dependent manner. Recombinant Sox9 associated with phosphorylated Smad3/4 and recognized the enhancer region of type II collagen gene. In vitro transcription and S1 nuclease assays showed that Smad3 and p300 cooperatively activated the Sox9-dependent transcription on chromatin template. The combination treatment of phosphorylated Smad3, Sox9, and p300 were necessary for the activation of chromatin-mediated transcription. These findings suggest that TGF-β signal Smad3 plays a key role for chromatin remodeling to induce chondrogenesis via its association with Sox9. en-copyright= kn-copyright= en-aut-name=FurumatsuTakayuki en-aut-sei=Furumatsu en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AsaharaHiroshi en-aut-sei=Asahara en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Orthopaedic Surgery, Okayama University Graduate School affil-num=2 en-affil= kn-affil=Department of Orthopaedic Surgery, Okayama University Graduate School affil-num=3 en-affil= kn-affil=Department of Molecular and Experimental Medicine, The Scripps Research Institute en-keyword=Chondrogenesis kn-keyword=Chondrogenesis en-keyword=Chromatin kn-keyword=Chromatin en-keyword=p300 kn-keyword=p300 en-keyword=Smad3 kn-keyword=Smad3 en-keyword=Sox9 kn-keyword=Sox9 END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=1 article-no= start-page=148 end-page=156 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Scleraxis and E47 cooperatively regulate the Sox9-dependent transcription en-subtitle= kn-subtitle= en-abstract= kn-abstract=During musculoskeletal development, Sry-type HMG box 9 (Sox9) has a crucial role in mesenchymal condensation and chondrogenesis. On the other hand, a tissue-specific basic helix-loop-helix (bHLH) transcription factor Scleraxis (Scx) regulates the differentiation of tendon and ligament progenitors. Whereas these two transcription factors cooperatively participate in the determination of cellular lineages, the precise interaction between Sox9 and Scx remains unclear. We have previously demonstrated that the Sox9-dependent transcription is synergistically activated by several Sox9-associating molecules, such as p300 and Smad3, on chromatin. In this study, we investigated the function of Scx in the Sox9-dependent transcription. The expression of α1(II) collagen (Col2a1) gene was stimulated by an appropriate transduction of Sox9 and Scx. Scx and its partner E47, which dimerizes with other bHLH proteins, cooperatively enhanced the Sox9-dependent transcription in luciferase reporter assays. Coactivator p300 synergistically increased the activity of Sox9-regulated reporter gene, which contains promoter and enhancer regions of Col2a1, in the presence of Scx and E47. Immunoprecipitation analyses revealed that Scx and E47 formed a transcriptional complex with Sox9 and p300. Scx/E47 heterodimer also associated with a conserved E-box sequence (CAGGTG) in the Col2a1 promoter on chromatin. These findings suggest that Scx and E47 might modulate the primary chondrogenesis by associating with the Sox9-related transcriptional complex, and by binding to the conserved E-box on Col2a1 promoter. en-copyright= kn-copyright= en-aut-name=FurumatsuTakayuki en-aut-sei=Furumatsu en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShukunamiChisa en-aut-sei=Shukunami en-aut-mei=Chisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Amemiya-KudoMichiyo en-aut-sei=Amemiya-Kudo en-aut-mei=Michiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimanoHitoshi en-aut-sei=Shimano en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Orthopaedic Surgery, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Cellular Differentiation, Institute for Frontier Medical Sciences, Kyoto University affil-num=3 en-affil= kn-affil=Okinaka Memorial Institute for Medical Research, Toranomon Hospital affil-num=4 en-affil= kn-affil=Department of Internal Medicine, Endocrinology and Metabolism, Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences Center for Tsukuba Advanced Research Alliance, University of Tsukuba affil-num=5 en-affil= kn-affil=Department of Orthopaedic Surgery, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences en-keyword=E47 kn-keyword=E47 en-keyword=p300 kn-keyword=p300 en-keyword=Scleraxis kn-keyword=Scleraxis en-keyword=Sox9 kn-keyword=Sox9 en-keyword=Transcription kn-keyword=Transcription END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=8 article-no= start-page=768 end-page=774 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of exercise training on gingival oxidative stress in obese rats en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet. Design: Rats were divided into three groups (n = 14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress. Results: The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean +/- SD; 413 +/- 64) than the control (333 +/- 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 +/- 0.18) (p < 0.05) and a lower GSH/GSSG ratio (17.0 +/- 3.1) (p < 0.05) in gingival tissues, compared to the control (0.55 +/- 0.13 for 8-OHdG and 23.6 +/- 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 +/- 103) (p<0.001) and gingival 8-OHdG levels (0.69 +/- 0.17) (p = 0.012) than those without exercise training (1105 95 for ROM and 0.55 +/- 0.13 for 8-OHdG) at 8 weeks. Conclusions: Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats. en-copyright= kn-copyright= en-aut-name=AzumaTetsuji en-aut-sei=Azuma en-aut-mei=Tetsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TomofujiTakaaki en-aut-sei=Tomofuji en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=EndoYasumasa en-aut-sei=Endo en-aut-mei=Yasumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TamakiNaofumi en-aut-sei=Tamaki en-aut-mei=Naofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=EkuniDaisuke en-aut-sei=Ekuni en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IrieKoichiro en-aut-sei=Irie en-aut-mei=Koichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KasuyamaKenta en-aut-sei=Kasuyama en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KatoTomo en-aut-sei=Kato en-aut-mei=Tomo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MoritaManabu en-aut-sei=Morita en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Obesity kn-keyword=Obesity en-keyword=Exercise training kn-keyword=Exercise training en-keyword=Periodontal diseases kn-keyword=Periodontal diseases en-keyword=Oxidative stress kn-keyword=Oxidative stress END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=38 article-no= start-page=7989 end-page=7997 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090919 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthetic studies on pterin glycosides: the first synthesis of 2′-O-(α-d-glucopyranosyl)biopterin en-subtitle= kn-subtitle= en-abstract= kn-abstract=L-Rhamnose was led, in a 14-step-sequence, to N2-(N,N-dimethylaminomethylene)-1′-O-(4-methoxybenzyl)-3-[2-(4-nitrophenyl)ethyl]biopterin (23), an appropriately protected precursor for 2′-O-glycosylation, while 4,6-di-O-acetyl-2,3-di-O-(4-methoxybenzyl)-α-d-glucopyranosyl bromide (32), a novel glycosyl donor, was efficiently prepared from d-glucose in 8 steps. The first synthesis of 2′-O-(α-d-glucopyranosyl)biopterin (2a) was achieved by treatment of the key intermediate 23 with 32 in the presence of silver triflate and tetramethylurea, followed by successive removal of the protecting groups. en-copyright= kn-copyright= en-aut-name=HanayaTadashi en-aut-sei=Hanaya en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BabaHiroki en-aut-sei=Baba en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ToyotaHiroki en-aut-sei=Toyota en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoHiroshi en-aut-sei=Yamamoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=School of Pharmacy, Shujitsu University en-keyword=Pterin glycoside kn-keyword=Pterin glycoside en-keyword=Pteridine kn-keyword=Pteridine en-keyword=Glycosylation kn-keyword=Glycosylation en-keyword=Protecting group kn-keyword=Protecting group en-keyword=Total synthesis kn-keyword=Total synthesis END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=9 article-no= start-page=2090 end-page=2100 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Efficient total syntheses of natural pterin glycosides: limipterin and tepidopterin en-subtitle= kn-subtitle= en-abstract= kn-abstract=The key, versatile precursors N-2-(N,N-dimethylaminomethylene)-1'-O-(4-methoxybenzyl)-3-[2-(4-nitrophenyl)ethyl]biopterin (29a) and its ciliapterin analog (29b) were prepared, respectively, from D-xylose (in 14 steps) and L-xylose (in 11 steps). Treatment of 29a and 29b with 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-beta-D-glucopyranosyl bromide in the presence of silver triflate and tetramethylurea, followed by removal of the protecting groups, led to the first selective syntheses of limipterin (3) and tepidopterin (5), respectively. en-copyright= kn-copyright= en-aut-name=HanayaTadashi en-aut-sei=Hanaya en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BabaHiroki en-aut-sei=Baba en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ToyotaHiroki en-aut-sei=Toyota en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoHiroshi en-aut-sei=Yamamoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=School of Pharmacy, Shujitsu University en-keyword=pteridine kn-keyword=pteridine en-keyword=pterin glycoside kn-keyword=pterin glycoside en-keyword=glycosylation kn-keyword=glycosylation en-keyword=protecting group kn-keyword=protecting group END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue=2 article-no= start-page=922 end-page=940 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080115 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor studies. Part 4: Design, synthesis, antitumor activity, and molecular docking study of novel 2-substituted 2-deoxoflavin-5-oxides, 2-deoxoalloxazine-5-oxides, and their 5-deaza analogs en-subtitle= kn-subtitle= en-abstract= kn-abstract=Various novel 10-alkyl-2-deoxo-2-methylthioflavin-5-oxides and their 2-alkylamino derivatives were prepared by facile nitrosative cyclization of 6-(N-alkylanilino)-2-methylthiopyrimidin-4(3H)-ones followed by nucleophilic replacement of the 2-methylthio moiety by different amines, and acidic hydrolysis of the 2-methylthio moiety afforded the corresponding flavin derivatives. 2-Deoxo-2-methylthio-5-deazaalloxazines and 2-deoxo-2-methylthioalloxazine-5-oxides were also prepared by Vilsmeier reaction and by nitrosation of 6-anilino-2-methylthiopyrimidin-4(3H)-ones, respectively. Then, they were subjected to nucleophilic replacement with appropriate amines to produce the corresponding 2-alkylamino derivatives. Regiospecific N-3-alkylation of 2-deoxo-2-methylthioalloxazine-5-oxides was carried out with various alkylating agents in the usual way, The antitumor activities against CCRF-HSB-2 and KB tumor cells have been investigated in vitro, and many compounds showed promising antitumor activities. Furthermore, AutoDock molecular docking into PTK (PDB: 1t46) has been done for lead optimization of the aforementioned compounds as potential PTK inhibitors. en-copyright= kn-copyright= en-aut-name=AliHamed I. en-aut-sei=Ali en-aut-mei=Hamed I. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AshidaNoriyuki en-aut-sei=Ashida en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=3 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=antitumor activity kn-keyword=antitumor activity en-keyword=flavin-5-oxide kn-keyword=flavin-5-oxide en-keyword=alloxazine-5-oxide kn-keyword=alloxazine-5-oxide en-keyword=AutoDock kn-keyword=AutoDock END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=1 article-no= start-page=242 end-page=256 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor studies. Part 1: Design, synthesis, antitumor activity, and AutoDock study of 2-deoxo-2-phenyl-5-deazaflavins and 2-deoxo-2-phenylflavin-5-oxides as a new class of antitumor agents en-subtitle= kn-subtitle= en-abstract= kn-abstract=Novel 2-deoxo-2-phenyl-5-deazaflavins and 2-deoxo-2-phenylflavin-5-oxides were prepared as a new class of antitumor agents and showed significant antitumor activities against NCI-H 460, HCT 116, A 431, CCRF-HSB-2, and KB cell lines. In vivo investigation, 2-deoxo-10-methyl-2-phenyl-5-deazaflavin exhibited the effective antitumor activity against A 431 human adenocarcinoma cells transplanted subcutaneously into nude mouse. Furthermore, AutoDock study has been done by binding of the flavin analogs into PTK pp60(c-src), where a good correlation between their IC50 and AutoDock binding free energy was exhibited. In particular, 2-deoxo-2-phenylflavin-5-oxides exhibited the highest potential binding affinity within the binding pocket of PTK. en-copyright= kn-copyright= en-aut-name=AliHamed I.. en-aut-sei=Ali en-aut-mei=Hamed I.. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TomitaKeiichiro en-aut-sei=Tomita en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkahoEiichi en-aut-sei=Akaho en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KambaraHiroto en-aut-sei=Kambara en-aut-mei=Hiroto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiuraShinji en-aut-sei=Miura en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HayakawaHiroyuki en-aut-sei=Hayakawa en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AshidaNoriyuki en-aut-sei=Ashida en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KawashimaYutaka en-aut-sei=Kawashima en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamagishiTakehiro en-aut-sei=Yamagishi en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IkeyaHisao en-aut-sei=Ikeya en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=YonedaFumio en-aut-sei=Yoneda en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Faculty of Pharmaceutical Sciences, High Technology Research Center (Life Science Center), and Center for Area Research and Development, Kobe Gakuin University affil-num=4 en-affil= kn-affil=Faculty of Pharmaceutical Sciences, High Technology Research Center (Life Science Center), and Center for Area Research and Development, Kobe Gakuin University affil-num=5 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=6 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=7 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=8 en-affil= kn-affil=Medicinal Research Laboratories, Taisho Pharmaceutical Co. affil-num=9 en-affil= kn-affil=Medicinal Research Laboratories, Taisho Pharmaceutical Co. affil-num=10 en-affil= kn-affil=Medicinal Research Laboratories, Taisho Pharmaceutical Co. affil-num=11 en-affil= kn-affil=Faculty of Pharmaceutical Sciences, Kyoto University affil-num=12 en-affil= kn-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=antitumor activity kn-keyword=antitumor activity en-keyword=Flavin analog kn-keyword=Flavin analog en-keyword=AutoDock kn-keyword=AutoDock en-keyword=protein tyrosine kinase kn-keyword=protein tyrosine kinase END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=19 article-no= start-page=6336 end-page=6352 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20071001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antitumor studies. Part 3: Design, synthesis, antitumor activity, and molecular docking study of novel 2-methylthio-, 2-amino-, and 2-(N-substituted amino)-10-alkyl-2-deoxo-5-deazaflavins en-subtitle= kn-subtitle= en-abstract= kn-abstract=Various novel 10-alkyl-2-deoxo-2-methylthio-5-deazaflavins have been synthesized by reaction of 6-(N-alkylanilino)-2-methylthiopyrimidin-4(3H)-ones with Vilsmeier reagent. The similar 2-(N-substituted amino) derivatives were prepared by nucleophilic replacement reaction of the 2-methylthio moiety by appropriate amines. The 2-oxo derivatives (i.e., 5-deazaflavins) were obtained by acidic hydrolysis of the 2-methylthio derivatives. The antitumor activities against CCRF-HSB-2 and KB cells and the antiviral activities against HSV-1 and HSV-2 have been investigated in vitro, and many compounds showed promising antitumor activities. Furthermore, AutoDock molecular docking into PTK has been done for lead optimization of these compounds as potential PTK inhibitors. Whereas, the designed 2-deoxo-5-deazaflavins connected with amino acids at the 2-position exhibited the good binding affinities into PTK with more hydrogen bonds. en-copyright= kn-copyright= en-aut-name=AliHamed I. en-aut-sei=Ali en-aut-mei=Hamed I. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AshidaNoriyuki en-aut-sei=Ashida en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagamatsuTomohisa en-aut-sei=Nagamatsu en-aut-mei=Tomohisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Biology Laboratory, Research and Development Division, Yamasa Shoyu Co. affil-num=3 en-affil= kn-affil=aDepartment of Drug Discovery and Development, Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=antitumor activity kn-keyword=antitumor activity en-keyword=5-deazaflavin kn-keyword=5-deazaflavin en-keyword=AutoDock kn-keyword=AutoDock en-keyword=protein tyrosine kn-keyword=protein tyrosine en-keyword=kinase kn-keyword=kinase END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=6 article-no= start-page=1021 end-page=1025 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Alkaline hydrothermal treatment of brominated high impact polystyrene (HIPS-Br) for bromine and bromine-free plastic recovery en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A method to recover both Br and Br-free plastic from brominated flame retardant high impact polystyrene (HIPS-Br) was proposed. HIPS-Br containing 15% Br was treated in autoclave at 280℃ using water or KOH solution of various amounts and concentrations. Hydrothermal treatment (30 ml water) leads to 90% debromination of 1 g HIPS-Br but plastic is strongly degraded and could not be recovered. previous termAlkalinenext term hydrothermal treatment (45 ml or 60 ml KOH 1 M) showed similar debromination for up to 12 g HIPS-Br and plastic was recovered as pellets with molecular weight distribution close to that of the initial material. Debromination occurs at melt plastic/KOH solution interface when liquid/vapour equilibrium is attained inside autoclave (280℃ and 7 MPa in our experimental conditions) and depends on the plastic amount/KOH volume ratio. The antimony oxide synergist from HIPS-Br remains in recovered plastic during treatment. A pictorial imagination of the proposed debromination process is presented.

en-copyright= kn-copyright= en-aut-name=BrebuMihai en-aut-sei=Brebu en-aut-mei=Mihai kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BhaskarThallada en-aut-sei=Bhaskar en-aut-mei=Thallada kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MutoAkinori en-aut-sei=Muto en-aut-mei=Akinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakataYusaku en-aut-sei=Sakata en-aut-mei=Yusaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Petru Poni Institute of Macromolecular Chemistry affil-num=2 en-affil= kn-affil=Department of Applied Chemistry, Faculty of Engineering, Okayama University affil-num=3 en-affil= kn-affil=Department of Applied Chemistry, Faculty of Engineering, Okayama University affil-num=4 en-affil= kn-affil=Department of Applied Chemistry, Faculty of Engineering, Okayama University en-keyword=Debromination kn-keyword=Debromination en-keyword=Alkaline hydrotreatment kn-keyword=Alkaline hydrotreatment en-keyword=HIPS-Br kn-keyword=HIPS-Br en-keyword=Bromine recovery kn-keyword=Bromine recovery END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=8 article-no= start-page=2120 end-page=2124 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200907 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The use of graphite oxide to produce mesoporous carbon supporting Pt, Ru, or Pd nanoparticles en-subtitle= kn-subtitle= en-abstract= kn-abstract=Mesoporous carbon having platinum, ruthenium or palladium nanoparticles on exfoliated graphene sheets were produced from graphite oxide (GO) and metal complexes. The Pt included carbon was made by heating of the intercalation compound including tetraammineplatinum (II) chloride monohydrate. Samples having Ru or Pd are producible by heating in nitrogen gas atmosphere using hexaammineruthenium (III) chloride or tetraamminepalladium (II) chloride monohydrate instead of Pt complex. The particle sizes of platinum, ruthenium, and palladium were, respectively, 1–3, 1–2, and 3–7 nm. The platinum- or palladium-containing sample showed catalytic activity for oxygen reduction. en-copyright= kn-copyright= en-aut-name=GotohKazuma en-aut-sei=Gotoh en-aut-mei=Kazuma kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawabataKoji en-aut-sei=Kawabata en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiiEiji en-aut-sei=Fujii en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MorishigeKunimitsu en-aut-sei=Morishige en-aut-mei=Kunimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KinumotoTaro en-aut-sei=Kinumoto en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyazakiYuki en-aut-sei=Miyazaki en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IshidaHiroyuki en-aut-sei=Ishida en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Industrial Technology Center of Okayama Prefecture affil-num=3 en-affil= kn-affil=Industrial Technology Center of Okayama Prefecture affil-num=4 en-affil= kn-affil=Okayama University of Science affil-num=5 en-affil= kn-affil=Faculty of Engineering, Oita University affil-num=6 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=24 article-no= start-page=5103 end-page=5111 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20041215 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A theoretical interpretation of the chemical shift of Si-29 NMR peaks in alkali borosilicate glasses en-subtitle= kn-subtitle= en-abstract= kn-abstract=In Si-29-NMR, it has so far been accepted that the chemical shifts of Q(n) Species (SiO4 units containing n bridging oxygens) were equivalent between alkali borosilicate, and boron-free alkali silicate classes. In the sodium borosilicate glasses with low sodium content. however. a contradiction was confirmed in the estimation of alkali distributions B-11 NMR suggested that Na ions were entirely distributed to berate groups to form BO4 units, whereas a -90 ppm component Was also observed in Si-29-NMR spectra, which has been attributed to Q(3) species associated with a nonbridging oxygen (NBO). Then. cluster molecular orbital calculations were performed to interpret the -90 ppm component in the borosilicate, glasses. It Was found that a silicon atom which had two tetrahedral borons (B4) as its second nearest neighbors was similar in atomic charge and Si2p energy to the Q(3) species in boron-free alkali silicates. Unequal distribution of electrons in Si-O-B4 bridging bonds was also found. where much electrons Were localized oil the Si-O bonds. It was finally concluded that the Si-O-B4 bridges with narrow bond angle were responsible for the -90 ppm Si-29 component in the borosilicate glasses. There still remained another interpretation: the Q(3) species were actually present in the glasses. and NBOs in the Q(3) species were derived from the tricluster groups. such as (O3Si)O(BO3)(2). In the classes With low sodium content. however. it was concluded that the tricluster groups were not so abundant to contribute to the -90 ppm component. en-copyright= kn-copyright= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishimuraMitsunori en-aut-sei=Nishimura en-aut-mei=Mitsunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiuraYoshinari en-aut-sei=Miura en-aut-mei=Yoshinari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=short-range order kn-keyword=short-range order en-keyword=nuclear-magnetic-resonance kn-keyword=nuclear-magnetic-resonance en-keyword=mas-nmr kn-keyword=mas-nmr en-keyword=structural kn-keyword=structural en-keyword=groups kn-keyword=groups en-keyword=oxygen sites kn-keyword=oxygen sites en-keyword=ab-initio kn-keyword=ab-initio en-keyword=o-17 nmr kn-keyword=o-17 nmr en-keyword=b-11 kn-keyword=b-11 en-keyword=na20-b2-3-s1-2 kn-keyword=na20-b2-3-s1-2 en-keyword=spectroscopy kn-keyword=spectroscopy END