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ID 60274
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Telengech, Paul Institute of Plant Science and Resources, Okayama University
Hisano, Sakae Institute of Plant Science and Resources, Okayama University
Mugambi, Cyrus Institute of Plant Science and Resources, Okayama University
Hyodo, Kiwamu Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Arjona-Lopez, Juan Manuel Institute of Plant Science and Resources, Okayama University
Lopez-Herrera, Carlos Jose Institute for Sustainable Agriculture,Spanish Research Council
Kanematsu, Satoko Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO)
Kondo, Hideki Institute of Plant Science and Resources, Okayama University Kaken ID publons researchmap
Suzuki, Nobuhiro Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Abstract
Partitiviruses (dsRNA viruses, familyPartitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus,Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16R. necatrixstrains belonging to 15 new species, for which "Rosellinia necatrix partitivirus 11-Rosellinia necatrix partitivirus 25" were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera,Alphapartitivirus, andBetapartitivirus. Some partitiviruses were transfected into reference strains of the natural host,R. necatrix, and an experimental host,Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns ofR. necatrixandC. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.
Keywords
partitivirus
dsRNA virus
phytopathogenic fungus
Rosellinia necatrix
Cryphonectria parasitica
diversity
reassortment
horizontal transfer
Published Date
2020-06-26
Publication Title
Frontiers in Microbiology
Volume
volume11
Publisher
Frontiers Media
Start Page
1064
ISSN
1664-302X
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 Telengech et al.
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PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.3389/fmicb.2020.01064
License
https://creativecommons.org/licenses/by/4.0/
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
17H01463
16H06436
16H06429
16K21723