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Kataoka, Kensuke Department of Systems BioMedicine, Tokyo Medical and Dental University
Kurimoto, Ryota Department of Systems BioMedicine, Tokyo Medical and Dental University
Tsutsumi, Hiroki Department of Systems BioMedicine, Tokyo Medical and Dental University
Chiba, Tomoki Department of Systems BioMedicine, Tokyo Medical and Dental University
Kato, Tomomi Department of Systems BioMedicine, Tokyo Medical and Dental University
Shishido, Kana Department of Systems BioMedicine, Tokyo Medical and Dental University
Kato, Mariko Department of Systems BioMedicine, Tokyo Medical and Dental University
Ito, Yoshiaki Department of Systems BioMedicine, Tokyo Medical and Dental University
Cho, Yuichiro Anatomy and Physiological Science, Tokyo Medical and Dental University
Hoshi, Osamu Anatomy and Physiological Science, Tokyo Medical and Dental University
Mimata, Ayako Research Core, Tokyo Medical and Dental University
Sakamaki, Yuriko Research Core, Tokyo Medical and Dental University
Nakamichi, Ryo Department of Systems BioMedicine, Tokyo Medical and Dental University
Lotz, Martin K. Department of Molecular Medicine, The Scripps Research Institute
Naruse, Keiji Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Asahara, Hiroshi Department of Systems BioMedicine, Tokyo Medical and Dental University
Abstract
Tendons and ligaments are pivotal connective tissues that tightly connect muscle and bone. In this study, we developed a novel approach to generate tendon/ligament-like tissues with a hierarchical structure, by introducing the tendon/ligament-specific transcription factor Mohawk (MKX) into the mesenchymal stem cell (MSC) line C3H10T1/2 cells, and by applying an improved three-dimensional (3D) cyclic mechanical stretch culture system. In our developed protocol, a combination of stableMkxexpression and cyclic mechanical stretch synergistically affects the structural tendon/ligament-like tissue generation and tendon related gene expression. In a histological analysis of these tendon/ligament-like tissues, an organized extracellular matrix (ECM), containing collagen type III and elastin, was observed. Moreover, we confirmed thatMkxexpression and cyclic mechanical stretch, induced the alignment of structural collagen fibril bundles that were deposited in a fibripositor-like manner during the generation of our tendon/ligament-like tissues. Our findings provide new insights for the tendon/ligament biomaterial fields.
Keywords
Mohawk
tendon
ligament
tissue engineering
mechanical-stress
Published Date
2020-06-02
Publication Title
Frontiers in Cell and Developmental Biology
Volume
volume8
Publisher
Frontiers Media
Start Page
307
ISSN
2296-634X
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 Kataoka, Kurimoto, Tsutsumi, Chiba, Kato, Shishido, Kato, Ito, Cho, Hoshi, Mimata, Sakamaki, Nakamichi, Lotz, Naruse and Asahara.
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publisher
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.3389/fcell.2020.00307
License
https://creativecommons.org/licenses/by/4.0/
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development