ID | 47026 |
JaLCDOI | |
Sort Key | 7
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FullText URL | |
Author |
Yamagiwa, Masashi
Namba, Akitoshi
Akao, Tetsuyuki
Mizuki, Eiichi
Ohba, Michio
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Abstract | The crystal proteins produced by Bacillus thuringiensis subsp, israelensis (Bti) and subsp. coreanensis A1519 strain were examined for the cytotoxicity against MOLT-4 and HeLa cells by MTT assay and LDH assay, The A1519 crystal proteins processed by proteinase K exhibited the specific cell-killing activity toward MOLT-4 with little damage to the cell membrane, On the other hand, the Bti crystal proteins processed by proteinase K caused the substantial damage to the cell membrane of both MOLT-4 and HeLa, leading to the cell lysis. The non-digested crystal proteins of both strains exhibited no cytotoxicity, These data suggested that while the Bti crystal proteins caused the colloid-osmotic swelling and cell lysis of MOLT-4 and HeLa, the proteinase K-digested A1519 crystal proteins induced the specific cell death of MOLT-4 through a mechanism other than that of Bti.
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Publication Title |
Memoirs of the Faculty of Engineering, Okayama University
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Published Date | 2002-03
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Volume | volume36
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Issue | issue2
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Publisher | Faculty of Engineering, Okayama University
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Start Page | 61
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End Page | 66
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ISSN | 0475-0071
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NCID | AA10699856
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Content Type |
Departmental Bulletin Paper
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OAI-PMH Set |
岡山大学
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language |
English
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File Version | publisher
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NAID | |
Eprints Journal Name | mfe
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