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ID 60464
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Author
Shen, Lianhua Collaborative Research Center (OMIC)
Yamamoto, Takushi Analytical & Measuring Instruments Division, Shimadzu Corporation
Tan, Xian Wen Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Ogata, Koretsugu Analytical & Measuring Instruments Division, Shimadzu Corporation
Ando, Eiji Analytical & Measuring Instruments Division, Shimadzu Corporation
Ozeki, Eiichi Technology Research Laboratory, Shimadzu Corporation
Matsuura, Eiji Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences ORCID Kaken ID publons researchmap
Abstract
Background and aimsDysregulated lipid metabolism has emerged as one of the major risk factors of atherosclerosis. Presently, there is a consensus that oxidized LDL (oxLDL) promotes development of atherosclerosis and downstream chronic inflammatory responses. Due to the dynamic metabolic disposition of lipoprotein, conventional approach to purify bioactive lipids for subsequent comprehensive analysis has proven to be inadequate for elucidation of the oxidized lipids species accountable for pathophysiology of atherosclerotic lesions. Herein, we aimed to utilize a novel mass microscopic imaging technology, coupled with mass spectrometry (MS) to characterize oxidized lipids in atherosclerotic lesions. MethodsWe attempted to use MALDI-TOF-MS and iMScope to identify selected oxidized lipid targets and visualize their respective localizations in study models of atherosclerosis. ResultsBased on the MS analysis, detection of 7-K under positive ionization through product ion peak at m/z 383 [M+H-H2O] indicated the distinctive presence of targeted lipid within Cu2+-oxLDL and Cu2+-oxLDL loaded macrophage-like J774A.1 cell, along with other cholesterol oxidation products. Moreover, the application of two-dimensional iMScope has successfully visualized the localization of lipids in aortic atherosclerotic plaques of the Watanabe heritable hyperlipidemic (WHHL) rabbit. Distinctive lipid distribution profiles were observed in atherosclerotic lesions of different sizes, especially the localizations of lysoPCs in atherosclerotic plaques. ConclusionsTaken together, we believe that both MALDI-TOF-MS and iMScope metabolomics technology may offer a novel proposition for future pathophysiological studies of lipid metabolism in atherosclerosis.
Keywords
Atherosclerosis
Low-density lipoprotein (LDL)
Oxidized LDL (oxLDL)
Oxidized lipids;
Imaging mass microscopy (iMScope)
Mass spectroscopy (MS)
Published Date
2020-08-28
Publication Title
Atherosclerosis
Volume
volume311
Publisher
Elsevier
Start Page
1
End Page
12
ISSN
00219150
NCID
AA00553457
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
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author
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DOI
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isVersionOf https://doi.org/10.1016/j.atherosclerosis.2020.08.001
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
Open Access (Publisher)
non-OA
Open Archive (publisher)
Non-OpenArchive