start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=8
article-no=
start-page=e02306
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190831
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effectiveness and safety of low-concentrated ozonized water for the reduction of contamination in dental unit water lines
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Contamination of dental unit waterlines (DUWL) with heterotrophic bacteria can cause problems in immune compromised patients (aged, tumor and organ transplantation-patients). We focused on the use of low-concentrated ozonized water (OZW) as the biofilm formation restraint system for DUWL. Here, we examined the effects of low-concentrated OZW on the growth of bacteria and related biofilm formation and harmfulness to dental unit components (DUCs) in vitro.
Objectives
To evaluate the bactericidal effects of OZW on biofilms in DUWL and DUC in vitro. Methods
Low-concentrated OZW (0.4 mg/L) was generated using an OZS-PTDX generator. Heterotrophic bacterial biofilms in old DUWL tubes and Candia albicans solution (control microbe) were treated with OZW for 1 h with gentle agitation before static culturing for 96 h in Reasoner's 2A liquid media. The control solutions were 0.1% cetylpyridinium chloride (CPC), chlorinated tap water (TW), and phosphate-buffered saline (PBS). Adenosine triphosphate (ATP) amounts of the microbes were measured and the biofilms of these microbes were observed using scanning electron microscopy (SEM). Moreover, surfaces of DUC soaked in OZW and TW were observed by SEM.
Results
The OZW reduced ATP levels in microbes to 50% compared to TW and PBS treatment, although CPC reduced it below detection limits. SEM observation revealed deformation of microbes cultured with OZW, whereas no changes were seen on DUC surfaces.
Conclusions
Low-concentrated OZW is bactericidal against heterotrophic bacteria biofilms and it is not harmful to DUC, suggesting that it might be useful in preventing DUWL contamination.
en-copyright=
kn-copyright=
en-aut-name=Okubo Keisuke
en-aut-sei=Okubo
en-aut-mei= Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=Ito Takashi
en-aut-sei=Ito
en-aut-mei= Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Shiota Yasuyoshi
en-aut-sei=Shiota
en-aut-mei= Yasuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=Kawata Yusuke
en-aut-sei=Kawata
en-aut-mei= Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=Yamamoto Tadashi
en-aut-sei=Yamamoto
en-aut-mei= Tadashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=Takashiba Shogo
en-aut-sei=Takashiba
en-aut-mei= Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=
affil-num=3
en-affil=Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Biofilm
kn-keyword=Biofilm
en-keyword=Dental chair unit water line (DUWL)
kn-keyword=Dental chair unit water line (DUWL)
en-keyword=Heterotrophic bacteria
kn-keyword=Heterotrophic bacteria
en-keyword=Low-concentration
kn-keyword=Low-concentration
en-keyword=Materials science
kn-keyword=Materials science
en-keyword=Ozonized water
kn-keyword=Ozonized water
END
start-ver=1.4
cd-journal=joma
no-vol=5
cd-vols=
no-issue=6
article-no=
start-page=e01936
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Vision evaluation by functional observational battery, operant behavior test, and light/dark box test in retinal dystrophic RCS rats versus normal rats
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=BACKGROUND:
Vision plays a key role in some behavior tests for rats. Okayama University-type retinal prosthesis (OUReP) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to assess vision in retinal dystrophic (RCS) rats, in comparison with normal rats, by selected behavior tests. We also examined whether the tests could detect vision changes in RCS rats with dye-coupled film implantation.
METHODS:
Data sets were 5 normal rats, 4 untreated RCS rats, 7 RCS rats with dye-coupled films implanted at the age of 7 weeks after excluding unsuccessful implantation at autopsy. Behavior tests chosen were landing foot splay and visual forelimb-placing response in the menu of functional observational battery, operant-conditioning lever-press response and light/dark box test.
RESULTS:
Normal visual placing response was significantly less frequent in untreated RCS rats at the age of 9 and 11 weeks, compared with normal rats (P = 0.0027, chi-square test) while normal response was significantly more frequent at the age of 9 weeks in RCS rats with dye-coupled film implantation, compared with untreated RCS rats (P = 0.0221). In operant-conditioning lever-press test, the correct response rate was significantly lower in untreated RCS rats than in normal rats at the age of 9 weeks (P < 0.05, Tukey-Kramer test) while the rate was not significantly different between normal rats and RCS rats with dye-coupled film implantation. In light/dark box test, the time to enter dark box was significantly shorter in normal rats, compared with untreated RCS rats or RCS rats with dye-coupled film implantation (P < 0.05, Tukey-Kramer test).
CONCLUSIONS:
Behavior tests of functional observational battery, operant-conditioning lever-press response and light/dark box test discriminated vision between normal rats and RCS rats. The visual placing response and operant-conditioning lever-press test might have sensitivity to detect vision recovery in RCS rats with OUReP implantation.
en-copyright=
kn-copyright=
en-aut-name=MatsuoToshihiko
en-aut-sei=Matsuo
en-aut-mei=Toshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UchidaTetsuya
en-aut-sei=Uchida
en-aut-mei=Tetsuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=YamashitaKoichiro
en-aut-sei=Yamashita
en-aut-mei=Koichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=Takei Shigiko
en-aut-sei=Takei
en-aut-mei= Shigiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=Ido Daisuke
en-aut-sei=Ido
en-aut-mei= Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=Fujiwara Atsushi
en-aut-sei=Fujiwara
en-aut-mei= Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=Iino Masahiko
en-aut-sei=Iino
en-aut-mei= Masahiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=Oguchi Masao
en-aut-sei=Oguchi
en-aut-mei= Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Ophthalmology, Okayama University Medical School and Okayama University Hospital
kn-affil=
affil-num=2
en-affil=Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology
kn-affil=
affil-num=3
en-affil=Polymer Materials Science, Okayama University Graduate School of Natural Science and Technology
kn-affil=
affil-num=4
en-affil=Ina Research
kn-affil=
affil-num=5
en-affil=Ina Research
kn-affil=
affil-num=6
en-affil=Ina Research
kn-affil=
affil-num=7
en-affil=Ina Research
kn-affil=
affil-num=8
en-affil=Ina Research
kn-affil=
en-keyword=Bioengineering
kn-keyword=Bioengineering
en-keyword=Neuroscience
kn-keyword=Neuroscience
en-keyword=Physiology
kn-keyword=Physiology
en-keyword=Zoology
kn-keyword=Zoology
END
start-ver=1.4
cd-journal=joma
no-vol=17
cd-vols=
no-issue=6
article-no=
start-page=e56
end-page=e57
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190531
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Myoepithelial Hamartoma in the Ampulla of Vater
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=TomodaTakeshi
en-aut-sei=Tomoda
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KatoHironari
en-aut-sei=Kato
en-aut-mei=Hironari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OkadaHiroyuki
en-aut-sei=Okada
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Department of Gastroenterology, Okayama University Hospital
kn-affil=
affil-num=2
en-affil=Department of Gastroenterology, Okayama University Hospital
kn-affil=
affil-num=3
en-affil=Department of Gastroenterology, Okayama University Hospital
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=262
cd-vols=
no-issue=
article-no=
start-page=37
end-page=47
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190331
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Insects are the host and vector of diverse viruses including those that infect vertebrates, plants, and fungi. Recent wide-scale transcriptomic analyses have uncovered the existence of a number of novel insect viruses belonging to an alphavirus-like superfamily (virgavirus/negevirus-related lineage). In this study, through an in silico search using publicly available insect transcriptomic data, we found numerous virus-like sequences related to insect virga/nege-like viruses. Phylogenetic analysis showed that these novel viruses and related virus-like sequences fill the major phylogenetic gaps between insect and plant virga/negevirus lineages. Interestingly, one of the phylogenetic clades represents a unique insect-infecting virus group. Its members encode putative coat proteins which contained a conserved domain similar to that usually found in the coat protein of plant viruses in the family Virgaviridae. Furthermore, we discovered endogenous viral elements (EVEs) related to virga/nege-like viruses in the insect genomes, which enhances our understanding on their evolution. Database searches using the sequence of one member from this group revealed the presence of EVEs in a wide range of insect species, suggesting that there has been prevalent infection by this virus group since ancient times. Besides, we present detailed EVE integration profiles of this virus group in some species of the Bombus genus of bee families. A large variation in EVE patterns among Bombus species suggested that while some integration events occurred after the species divergence, others occurred before it. Our analyses support the view that insect and plant virga/nege-related viruses might share common virus origin(s).
en-copyright=
kn-copyright=
en-aut-name=Kondo Hideki
en-aut-sei=Kondo
en-aut-mei= Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=Chiba Sotaro
en-aut-sei=Chiba
en-aut-mei= Sotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Maruyama Kazuyuki
en-aut-sei=Maruyama
en-aut-mei= Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=Andika Ida Bagus
en-aut-sei=Andika
en-aut-mei= Ida Bagus
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=Suzuki Nobuhiro
en-aut-sei=Suzuki
en-aut-mei= Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=2
en-affil=Asian Satellite Campuses Institute, Nagoya University
kn-affil=
affil-num=3
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=4
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=5
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
en-keyword=Bumblebee
kn-keyword=Bumblebee
en-keyword=Endogenous viral element
kn-keyword=Endogenous viral element
en-keyword= Evolution
kn-keyword= Evolution
en-keyword=Insect
kn-keyword=Insect
en-keyword= Plant alpha-like virus
kn-keyword= Plant alpha-like virus
en-keyword=Transcriptome shotgun assembly
kn-keyword=Transcriptome shotgun assembly
en-keyword=Whole genome shotgun assembly
kn-keyword=Whole genome shotgun assembly
END
start-ver=1.4
cd-journal=joma
no-vol=1863
cd-vols=
no-issue=4
article-no=
start-page=672
end-page=680
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190117
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Regulation of Ca2+/calmodulin-dependent protein kinase kinase beta by cAMP signaling
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=BACKGROUND:
Ca2+/calmodulin-dependent protein kinase kinase (CaMKK) is a pivotal activator of CaMKI, CaMKIV and 5'-AMP-activated protein kinase (AMPK), controlling Ca2+-dependent intracellular signaling including various neuronal, metabolic and pathophysiological responses. Recently, we demonstrated that CaMKKβ is feedback phosphorylated at Thr144 by the downstream AMPK, resulting in the conversion of CaMKKβ into Ca2+/CaM-dependent enzyme. However, the regulatory phosphorylation of CaMKKβ at Thr144 in intact cells and in vivo remains unclear.
METHODS:
Anti-phosphoThr144 antibody was used to characterize the site-specific phosphorylation of CaMKKβ in immunoprecipitated samples from mouse cerebellum and in transfected mammalian cells that were treated with various agonists and protein kinase inhibitors. CaMKK activity assay and LC-MS/MS analysis were used for biochemical characterization of phosphorylated CaMKKβ.
RESULTS:
Our data suggest that the phosphorylation of Thr144 in CaMKKβ is rapidly induced by cAMP/cAMP-dependent protein kinase (PKA) signaling in CaMKKβ-transfected HeLa cells, that is physiologically relevant in mouse cerebellum. We confirmed that the catalytic subunit of PKA was capable of directly phosphorylating CaMKKβ at Thr144 in vitro and in transfected cells. In addition, the basal phosphorylation of CaMKKβ at Thr144 in transfected HeLa cells was suppressed by AMPK inhibitor (compound C). PKA-catalyzed phosphorylation reduced the autonomous activity of CaMKKβ in vitro without significant effect on the Ca2+/CaM-dependent activity, resulting in the conversion of CaMKKβ into Ca2+/CaM-dependent enzyme.
CONCLUSION:
cAMP/PKA signaling may confer Ca2+-dependency to the CaMKKβ-mediated signaling pathway through direct phosphorylation of Thr144 in intact cells.
GENERAL SIGNIFICANCE:
Our results suggest a novel cross-talk between cAMP/PKA and Ca2+/CaM/CaMKKβ signaling through regulatory phosphorylation.
en-copyright=
kn-copyright=
en-aut-name=TakabatakeShota
en-aut-sei=Takabatake
en-aut-mei=Shota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OhtsukaSatomi
en-aut-sei=Ohtsuka
en-aut-mei=Satomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=SugawaraTakeyuki
en-aut-sei=Sugawara
en-aut-mei=Takeyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=HatanoNaoya
en-aut-sei=Hatano
en-aut-mei=Naoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KanayamaNaoki
en-aut-sei=Kanayama
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MagariMasaki
en-aut-sei=Magari
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=SakagamiHiroyuki
en-aut-sei=Sakagami
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=TokumitsuHiroshi
en-aut-sei=Tokumitsu
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=2
en-affil=Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Anatomy, Kitasato University School of Medicine
kn-affil=
affil-num=4
en-affil=Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=5
en-affil=Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=6
en-affil=Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=7
en-affil=Department of Anatomy, Kitasato University School of Medicine
kn-affil=
affil-num=8
en-affil=Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
en-keyword=CaMKK
kn-keyword=CaMKK
en-keyword=Calmodulin
kn-keyword=Calmodulin
en-keyword=Intracellular Ca(2+)
kn-keyword=Intracellular Ca(2+)
en-keyword=PKA
kn-keyword=PKA
en-keyword=Phosphorylation
kn-keyword=Phosphorylation
en-keyword=Signal transduction
kn-keyword=Signal transduction
END
start-ver=1.4
cd-journal=joma
no-vol=913
cd-vols=
no-issue=
article-no=
start-page=72
end-page=77
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190101
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Energy response of X-rays under high flux conditions using a thin APD for the energy range of 6–33 keV
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= This paper reports on the demonstration of a high-rate energy measurement technique using a thin depletion layer silicon avalanche photodiode (Si-APD). A dedicated amplitude-to-time converter is developed to realize simultaneous energy and timing measurement in a high rate condition. The energy response of the system is systematically studied by using monochromatic X-ray beam with an incident energy ranging from 6 to 33 keV. The obtained energy spectra contain clear peaks and tail distributions. The peak fraction monotonously decreases as the incident photon energy increases. This phenomenon can be explained by considering the distribution of the energy deposit in silicon, which is investigated by using a Monte Carlo simulation.
en-copyright=
kn-copyright=
en-aut-name=MasudaT.
en-aut-sei=Masuda
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HirakiT.
en-aut-sei=Hiraki
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KainoH.
en-aut-sei=Kaino
en-aut-mei=H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KishimotoS.
en-aut-sei=Kishimoto
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=MiyamotoY.
en-aut-sei=Miyamoto
en-aut-mei=Y.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OkaiK.
en-aut-sei=Okai
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=OkuboS.
en-aut-sei=Okubo
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=OzakiR.
en-aut-sei=Ozaki
en-aut-mei=R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=SasaoN.
en-aut-sei=Sasao
en-aut-mei=N.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=SuzukiK.
en-aut-sei=Suzuki
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=UetakeS.
en-aut-sei=Uetake
en-aut-mei=S.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=YoshimiA.
en-aut-sei=Yoshimi
en-aut-mei=A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=YoshimuraK.
en-aut-sei=Yoshimura
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
affil-num=1
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=2
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=3
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=4
en-affil=High Energy Accelerator Research Organization
kn-affil=
affil-num=5
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=6
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=7
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=8
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=9
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=10
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=11
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=12
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
affil-num=13
en-affil=Research Institute for Interdisciplinary Science, Okayama University
kn-affil=
en-keyword=Avalanche photodiode
kn-keyword=Avalanche photodiode
en-keyword=X-ray
kn-keyword=X-ray
END
start-ver=1.4
cd-journal=joma
no-vol=484
cd-vols=
no-issue=
article-no=
start-page=394
end-page=401
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180925
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Syntheses and crystal structures of neodymium(III) and europium(III) complexes bearing dimethyl-, pyrrolidine-, or S-prolinol- dithiocarbamato ligands and their natural and magnetic circular dichroism spectra
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= A series of Nd-III and Eu-III complexes containing achiral or chiral dithiocarbamato (dtc) ligands, [Ln(Xdtc)(3)(NN)] {Ln = Nd or Eu; X = dimethyl- (Me-2), pyrrolidine- (pyr), or (S)-prolinol- (S-proOH); NN = 1,10-phenanthroline (phen) or 2,2'-bipyridine (bpy)}, were prepared and their crystal structures and spectroscopic properties, in particular the natural circular dichroism (CD) and magnetic circular dichroism (MCD), were investigated. The crystal structures of the complexes analyzed by the X-ray diffraction method showed an 8-coordinate geometry around the Ln III center with comparable structural parameters to one another and to the related complexes reported previously. These complexes exhibited similar spectral patterns in their absorption, natural CD and MCD spectra in solution. Weak but characteristic sharp f-f transition bands were observed in the absorption and MCD spectra, but no CD signals associated with these transitions were observed even in the S-proOHdtc complexes. The MCD spectral pattern of the Eu-III complexes revealed a local C-2v symmetry around the Ln(III) center in solution, in contrast to the aqua and the analogous beta-diketonato Eu-III complexes.
en-copyright=
kn-copyright=
en-aut-name=YakubuAbdallah
en-aut-sei=Yakubu
en-aut-mei=Abdallah
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=SuzukiTakayoshi
en-aut-sei=Suzuki
en-aut-mei=Takayoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KitaMasakazu
en-aut-sei=Kita
en-aut-mei=Masakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=
affil-num=3
en-affil=Faculty of Education, Okayama University
kn-affil=
en-keyword=Dithiocarbamate
kn-keyword=Dithiocarbamate
en-keyword=Lanthanoid
kn-keyword=Lanthanoid
en-keyword=Crystal structures
kn-keyword=Crystal structures
en-keyword=Magnetic circular dichroism
kn-keyword=Magnetic circular dichroism
en-keyword=(S)-prolinol dithiocarbamate
kn-keyword=(S)-prolinol dithiocarbamate
END
start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=
article-no=
start-page=154
end-page=164
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20171016
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Attenuation of CD4+ CD25+ Regulatory T Cells in the Tumor Microenvironment by Metformin, a Type 2 Diabetes Drug
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= CD4+CD25+ regulatory T cells (Treg), an essential subset for preventing autoimmune diseases, is implicated as a negative regulator in anti-tumor immunity. We found that metformin (Met) reduced tumor-infiltrating Treg (Ti-Treg), particularly the terminally-differentiated CD103+KLRG1+ population, and also decreased effector molecules such as CTLA4 and IL-10. Met inhibits the differentiation of naïve CD4+ T cells into inducible Treg (iTreg) by reducing forkhead box P3 (Foxp3) protein, caused by mTORC1 activation that was determined by the elevation of phosphorylated S6 (pS6), a downstream molecule of mTORC1. Rapamycin and compound C, an inhibitor of AMP-activated protein kinase (AMPK) restored the iTreg generation, further indicating the involvement of mTORC1 and AMPK. The metabolic profile of iTreg, increased Glut1-expression, and reduced mitochondrial membrane-potential and ROS production of Ti-Treg aided in identifying enhanced glycolysis upon Met-treatment. The negative impact of Met on Ti-Treg may help generation of the sustained antitumor immunity.
en-copyright=
kn-copyright=
en-aut-name=KunisadaYuki
en-aut-sei=Kunisada
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=EikawaShingo
en-aut-sei=Eikawa
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TomonobuNahoko
en-aut-sei=Tomonobu
en-aut-mei=Nahoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=DomaeShohei
en-aut-sei=Domae
en-aut-mei=Shohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=UeharaTakenori
en-aut-sei=Uehara
en-aut-mei=Takenori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=HoriShohei
en-aut-sei=Hori
en-aut-mei=Shohei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=FurusawaYukihiro
en-aut-sei=Furusawa
en-aut-mei=Yukihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=HaseKoji
en-aut-sei=Hase
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=SasakiAkira
en-aut-sei=Sasaki
en-aut-mei=Akira
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=UdonoHeiichiro
en-aut-sei=Udono
en-aut-mei=Heiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Laboratory of Immunology and Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
kn-affil=
affil-num=7
en-affil=Division of Biochemistry, Keio University Graduate School of Pharmaceutical Science
kn-affil=
affil-num=8
en-affil=Division of Biochemistry, Keio University Graduate School of Pharmaceutical Science
kn-affil=
affil-num=9
en-affil=Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=10
en-affil=Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=Glycolysis
kn-keyword=Glycolysis
en-keyword=Regulatory T cell (Treg)
kn-keyword=Regulatory T cell (Treg)
en-keyword=Tumor immunity
kn-keyword=Tumor immunity
en-keyword=Tumor microenvironment
kn-keyword=Tumor microenvironment
en-keyword=mTOR
kn-keyword=mTOR
END
start-ver=1.4
cd-journal=joma
no-vol=54
cd-vols=
no-issue=
article-no=
start-page=47
end-page=53
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201710
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Characterization of Vibrio cholerae O1 strains that trace the origin of Haitian-like genetic traits
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Vibrio cholerae O1 is the etiological agent of the severe diarrheal disease cholera. The bacterium has recently been causing outbreaks in Haiti with catastrophic effects. Numerous mutations have been reported in V. cholerae O1 strains associated with the Haitian outbreak. These mutations encompass among other the genes encoding virulence factors such as the pilin subunit of the toxin-co-regulated pilus (tcpA), cholera toxin B subunit (ctxB), repeat in toxins (rtxA), and other genes such as the quinolone resistance-determining region (QRDR) of gyrase A (gyrA), rstB of RS element along with the alteration in the number of repeat sequences at the promoter region of ctxAB. Given the numerous genetic changes in those Haitian isolates, we decided to investigate the possible origins of those variations in the Indian subcontinent. Thus, we determined the genetic traits among V. cholerae O1 strains in Delhi, India. A total of 175 strains isolated from cholera patients during 2004 to 2012 were analysed in the present study. Our results showed that all the tested strains carried Haitian type tcpA (tcpACIRS) and variant gyrA indicating their first appearance before 2004 in Delhi. The Haitian variant rtxA and ctxB7 were first detected in Delhi during 2004 and 2006, respectively. Interestingly, not a single strain with the combination of El Tor rtxA and ctxB7 was detected in this study. The Delhi strains carried four heptad repeats (TTTTGAT) in the CT promoter region whereas Haitian strains carried 5 such repeats. Delhi strains did not have any deletion mutations in the rstB like Haitian strains. Overall, our study demonstrates the sequential accumulation of Haitian-like genetic traits among V. cholerae O1 strains in Delhi at different time points prior to the Haitian cholera outbreak.
en-copyright=
kn-copyright=
en-aut-name=GhoshPriyanka
en-aut-sei=Ghosh
en-aut-mei=Priyanka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KumarDhirendra
en-aut-sei=Kumar
en-aut-mei=Dhirendra
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ChowdhuryGoutam
en-aut-sei=Chowdhury
en-aut-mei=Goutam
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=SinghPuneeta
en-aut-sei=Singh
en-aut-mei=Puneeta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SamantaProsenjit
en-aut-sei=Samanta
en-aut-mei=Prosenjit
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=DuttaShanta
en-aut-sei=Dutta
en-aut-mei=Shanta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=RamamurthyT.
en-aut-sei=Ramamurthy
en-aut-mei=T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=SharmaN. C.
en-aut-sei=Sharma
en-aut-mei=N. C.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=SinhaPreety
en-aut-sei=Sinha
en-aut-mei=Preety
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=PrasadYogendra
en-aut-sei=Prasad
en-aut-mei=Yogendra
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=ShinodaSumio
en-aut-sei=Shinoda
en-aut-mei=Sumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=MukhopadhyayAsish K.
en-aut-sei=Mukhopadhyay
en-aut-mei=Asish K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
affil-num=1
en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=2
en-affil=Maharishi Valmiki Infectious Diseases Hospital
kn-affil=
affil-num=3
en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=4
en-affil=Maharishi Valmiki Infectious Diseases Hospital
kn-affil=
affil-num=5
en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=6
en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=7
en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=8
en-affil=Maharishi Valmiki Infectious Diseases Hospital
kn-affil=
affil-num=9
en-affil=Department of Zoology, A.N. College
kn-affil=
affil-num=10
en-affil=Department of Animal Science, MJP Rohilkhand University
kn-affil=
affil-num=11
en-affil=Collaborative Research Center of Okayama University for Infectious Diseases at NICED
kn-affil=
affil-num=12
en-affil=Division of Bacteriology, National Institute of Cholera and Enteric Diseases
kn-affil=
en-keyword=Cholera
kn-keyword=Cholera
en-keyword=Vibrio cholerae
kn-keyword=Vibrio cholerae
en-keyword=ctxAB promoter
kn-keyword=ctxAB promoter
en-keyword=ctxB
kn-keyword=ctxB
en-keyword=gyrA
kn-keyword=gyrA
en-keyword=rstB
kn-keyword=rstB
en-keyword=rtxA
kn-keyword=rtxA
en-keyword=tcpA
kn-keyword=tcpA
END
start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=6
article-no=
start-page=812
end-page=818
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201706
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Predictors of vasovagal reactions during preoperative autologous blood donation: a single-institution analysis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Studies examining risk factors associated with vasovagal reactions (VVRs) during autologous blood donations, especially in younger subjects, have been limited. The aim of the present study was to define risk factors for VVRs during preoperative autologous blood donation in patients, including those younger than 18 years old. We retrospectively analyzed 4192 autologous, preoperative blood donations between 2007 and 2015 at Okayama University Hospital. Eighty-seven (2.08%) of the patients experienced VVRs. VVRs occurred approximately three times as often in patients 0-17 years old (16/320, 5.0%) than in patients 18 years and older (71/3872, 1.8%). In particular, VVRs occurred more frequently in those 10-13 years old, and decreased with older age (P = 0.006). In a univariate analysis, younger age, lower body mass index, lower systolic blood pressure, lower body weight, lower total blood volume, female gender, first-time collection, and higher heart rate were associated with a higher incidence of VVRs. In a multivariate analysis, lower systolic blood pressure (P < 0.001), higher heart rate (P = 0.007), and first-time collection (P = 0.015), remained independent predictors of VVRs. These results emphasize the need for careful attention during blood collection.
en-copyright=
kn-copyright=
en-aut-name=NishimoriHisakazu
en-aut-sei=Nishimori
en-aut-mei=Hisakazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=FujiiNobuharu
en-aut-sei=Fujii
en-aut-mei=Nobuharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=FujiiKeiko
en-aut-sei=Fujii
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=IkedaTohru
en-aut-sei=Ikeda
en-aut-mei=Tohru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=AsanoNaomi
en-aut-sei=Asano
en-aut-mei=Naomi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OgoHiroaki
en-aut-sei=Ogo
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=YamakawaMiwa
en-aut-sei=Yamakawa
en-aut-mei=Miwa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=TakagiNaoe
en-aut-sei=Takagi
en-aut-mei=Naoe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=OtsukaFumio
en-aut-sei=Otsuka
en-aut-mei=Fumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=IkedaKazuma
en-aut-sei=Ikeda
en-aut-mei=Kazuma
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=2
en-affil= Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=3
en-affil= Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=4
en-affil= Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=5
en-affil= Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=6
en-affil= Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=7
en-affil=Department of Nursing, Okayama University Hospital
kn-affil=
affil-num=8
en-affil=Department of Nursing, Okayama University Hospital
kn-affil=
affil-num=9
en-affil=Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
affil-num=10
en-affil=Department of Transfusion Medicine, Okayama University Hospital
kn-affil=
en-keyword=Autologous blood donation
kn-keyword=Autologous blood donation
en-keyword=Vasovagal reactions
kn-keyword=Vasovagal reactions
END
start-ver=1.4
cd-journal=joma
no-vol=33
cd-vols=
no-issue=3
article-no=
start-page=e124
end-page=e135
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201703
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Sandblasting may damage the surface of composite CAD-CAM blocks
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= OBJECTIVE:
CAD-CAM blocks to fabricate semi-direct and indirect restorations are available in different sorts of ceramics as well as composite. In order to bond restorations prepared out of composite blocks into tooth cavities, it is recommended to gently sandblast the surface prior to the application of a primer/adhesive. Today, the effect of sandblasting composite block surfaces has not thoroughly been investigated. In this study, the ultra-structure of composite CAD-CAM blocks was investigated with special attention to the effect of sandblasting on the surface topography and of silanization on the bonding performance.
METHODS:
Five different composite CAD-CAM blocks were involved. We correlatively investigated their structural and chemical composition using X-ray diffraction (XRD), energy dispersion spectroscopy (EDS), scanning electron microscopy (SEM) and (scanning) transmission electron microscopy ((S)TEM). The effect of sandblasting was also imaged in cross-section and at the interface with composite cement. Finally, we measured the shear bond strength to the sandblasted block surface with and without silanization.
RESULTS:
All composite blocks revealed a different ultra-structure. Sandblasting increased surface roughness and resulted in an irregular surface with some filler exposure. Sandblasting also damaged the surface. When the sandblasted composite blocks were silanized, superior bonding receptiveness in terms of higher bond strength was achieved except for Shofu Block HC.
SIGNIFICANCE:
Sandblasting followed by silanization improved the bond strength to composite CAD-CAM blocks. However, sandblasting may also damage the composite CAD-CAM block surface. For the composite CAD-CAM block Shofu Block HC, the damage was so severe that silanization did not improve bond strength.
en-copyright=
kn-copyright=
en-aut-name=YoshiharaKumiko
en-aut-sei=Yoshihara
en-aut-mei=Kumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=NagaokaNoriyuki
en-aut-sei=Nagaoka
en-aut-mei=Noriyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MaruoYukinori
en-aut-sei=Maruo
en-aut-mei=Yukinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NishigawaGoro
en-aut-sei=Nishigawa
en-aut-mei=Goro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=IrieMasao
en-aut-sei=Irie
en-aut-mei=Masao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=YoshidaYasuhiro
en-aut-sei=Yoshida
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MeerbeekfBart Van
en-aut-sei=Meerbeekf
en-aut-mei=Bart Van
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Centrer for Innovative Clinical Medicine, Okayama University Hospital
kn-affil=
affil-num=2
en-affil=Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School
kn-affil=
affil-num=3
en-affil= Department of Occlusion and Removable Prosthodontics, Okayama University Hospital
kn-affil=
affil-num=4
en-affil= Department of Occlusion and Removable Prosthodontics, Okayama University Hospital
kn-affil=
affil-num=5
en-affil=Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil= Department of Biomaterials and Bioengineering, Graduate School of Dental Medicine, Hokkaido University
kn-affil=
affil-num=7
en-affil= KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals
kn-affil=
en-keyword=CAD–CAM
kn-keyword=CAD–CAM
en-keyword=bond strength
kn-keyword=bond strength
en-keyword=composite
kn-keyword=composite
en-keyword=sandblast
kn-keyword=sandblast
en-keyword=silane coupling agent
kn-keyword=silane coupling agent
END
start-ver=1.4
cd-journal=joma
no-vol=43
cd-vols=
no-issue=6
article-no=
start-page=372
end-page=378
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=201606
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Noninvasive evaluation of nicotinic acetylcholine receptor availability in mouse brain using single-photon emission computed tomography with [(123)I]5IA.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=INTRODUCTION:
Nicotinic acetylcholine receptors (nAChRs) are of great interest because they are implicated in higher brain functions. Nuclear medical imaging is one of the useful techniques for noninvasive evaluation of physiological and pathological function in living subjects. Recent progress in nuclear medical imaging modalities enables the clear visualization of the organs of small rodents. Thus, translational research using nuclear medical imaging in transgenic mice has become possible and helps to elucidate human disease pathology. However, imaging of α4β2 nAChRs in the mouse brain has not yet been performed. The purpose of this study was to assess the feasibility of single-photon emission computed tomography (SPECT) with 5-[(123)I]iodo-3-[2(S)-azetidinylmethoxy]pyridine ([(123)I]5IA) for evaluating α4β2 nAChR availability in the mouse brain.
METHODS:
A 60-min dynamic SPECT imaging session of α4β2 nAChRs in the mouse brain was performed. The regional distribution of radioactivity in the SPECT images was compared to the density of α4β2 nAChRs measured in an identical mouse. Alteration of nAChR density in the brains of Tg2576 mice was also evaluated.
RESULTS:
The mouse brain was clearly visualized by [(123)I]5IA-SPECT and probe accumulation was significantly inhibited by pretreatment with (-)-nicotine. The regional distribution of radioactivity in SPECT images showed a significant positive correlation with α4β2 nAChR density measured in an identical mouse brain. Moreover, [(123)I]5IA-SPECT was able to detect the up-regulation of α4β2 nAChRs in the brains of Tg2576 transgenic mice.
CONCLUSIONS:
[(123)I]5IA-SPECT imaging would be a promising tool for evaluating α4β2 nAChR availability in the mouse brain and may be useful in translational research focused on nAChR-related diseases.
en-copyright=
kn-copyright=
en-aut-name=MatsuuraYuki
en-aut-sei=Matsuura
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UedaMasashi
en-aut-sei=Ueda
en-aut-mei=Masashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=HigakiYusuke
en-aut-sei=Higaki
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WatanabeKeiko
en-aut-sei=Watanabe
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=HabaraShogo
en-aut-sei=Habara
en-aut-mei=Shogo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KaminoShinichiro
en-aut-sei=Kamino
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=SajiHideo
en-aut-sei=Saji
en-aut-mei=Hideo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=EnomotoShuichi
en-aut-sei=Enomoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=6
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=7
en-affil=Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University
kn-affil=
affil-num=8
en-affil=Department of Pharmaceutical Analytical Chemistry, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=5-[(123)I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([(123)I]5IA)
kn-keyword=5-[(123)I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([(123)I]5IA)
en-keyword=Alzheimer's disease
kn-keyword=Alzheimer's disease
en-keyword=Mouse
kn-keyword=Mouse
en-keyword=Nicotinic acetylcholine receptor (nAChR)
kn-keyword=Nicotinic acetylcholine receptor (nAChR)
en-keyword=Single-photon emission computed tomography (SPECT)
kn-keyword=Single-photon emission computed tomography (SPECT)
en-keyword=Tg2576
kn-keyword=Tg2576
END
start-ver=1.4
cd-journal=joma
no-vol=213
cd-vols=
no-issue=
article-no=
start-page=353
end-page=364
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=201602
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Sequence and phylogenetic analyses of novel totivirus-like double-stranded RNAs from field-collected powdery mildew fungi
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= The identification of mycoviruses contributes greatly to understanding of the diversity and evolutionary aspects of viruses. Powdery mildew fungi are important and widely studied obligate phytopathogenic agents, but there has been no report on mycoviruses infecting these fungi. In this study, we used a deep sequencing approach to analyze the double-stranded RNA (dsRNA) segments isolated from field-collected samples of powdery mildew fungus-infected red clover plants in Japan. Database searches identified the presence of at least ten totivirus (genus Totivirus)-like sequences, termed red clover powdery mildew-associated totiviruses (RPaTVs). The majority of these sequences shared moderate amino acid sequence identity with each other (<44%) and with other known totiviruses (<59%). Nine of these identified sequences (RPaTV1a, 1b and 2-8) resembled the genome of the prototype totivirus, Saccharomyces cerevisiae virus-L-A (ScV-L-A) in that they contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA dependent RNA polymerase (RdRp), while one sequence (RPaTV9) showed similarity to another totivirus, Ustilago maydis virus H1 (UmV-H1) that encodes a single polyprotein (CP-RdRp fusion). Similar to yeast totiviruses, each ScV-L-A-like RPaTV contains a -1 ribosomal frameshift site downstream of a predicted pseudoknot structure in the overlapping region of these ORFs, suggesting that the RdRp is translated as a CP-RdRp fusion. Moreover, several ScV-L-A-like sequences were also found by searches of the transcriptome shotgun assembly (TSA) libraries from rust fungi, plants and insects. Phylogenetic analyses show that nine ScV-L-A-like RPaTVs along with ScV-L-A-like sequences derived from TSA libraries are clustered with most established members of the genus Totivirus, while one RPaTV forms a new distinct clade with UmV-H1, possibly establishing an additional genus in the family. Taken together, our results indicate the presence of diverse, novel totiviruses in the powdery mildew fungus populations infecting red clover plants in the field.
en-copyright=
kn-copyright=
en-aut-name=KondoHideki
en-aut-sei=Kondo
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HisanoSakae
en-aut-sei=Hisano
en-aut-mei=Sakae
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ChibaSotaro
en-aut-sei=Chiba
en-aut-mei=Sotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MaruyamaKazuyuki
en-aut-sei=Maruyama
en-aut-mei=Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=AndikaIda Bagus
en-aut-sei=Andika
en-aut-mei=Ida Bagus
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=FujimoriFumihiro
en-aut-sei=Fujimori
en-aut-mei=Fumihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=SuzukiNobuhiro
en-aut-sei=Suzuki
en-aut-mei=Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=2
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=3
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=4
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=5
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=7
en-affil=Department of Environmental Education, Tokyo Kasei University
kn-affil=
affil-num=8
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
en-keyword=Deep sequencing
kn-keyword=Deep sequencing
en-keyword=Double stranded RNA virus
kn-keyword=Double stranded RNA virus
en-keyword= Powdery mildew
kn-keyword= Powdery mildew
en-keyword=Saccharomyces cerevisiae virus L-A
kn-keyword=Saccharomyces cerevisiae virus L-A
en-keyword=Totivirus
kn-keyword=Totivirus
en-keyword=Ustilago maydis virus H1
kn-keyword=Ustilago maydis virus H1
END
start-ver=1.4
cd-journal=joma
no-vol=32
cd-vols=
no-issue=supplment 1
article-no=
start-page=A20
end-page=A28
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140811
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Hospital based surveillance and genetic characterization of rotavirus strains in children (<5 years) with acute gastroenteritis in Kolkata, India, revealed resurgence of G9 and G2 genotypes during 2011-2013
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=INTRODUCTION:
India accounts for an estimated 457,000-884,000 hospitalizations and 2 million outpatient visits for diarrhea. In spite of the huge burden of rotavirus (RV) disease, RV vaccines have not been introduced in national immunization programme of India. Therefore, continuous surveillance for prevalence and monitoring of the circulating genotypes is needed to assess the disease burden prior to introduction of vaccines in this region.
METHODS:
During January 2011 through December 2013, 830 and 1000 stool samples were collected from hospitalized and out-patient department (OPD) patients, respectively, in two hospitals in Kolkata, Eastern India. After primary screening, the G-P typing was done by multiplex semi-nested PCR using type specific primers followed by sequencing. Phylogenetic analysis for the VP7 gene of 25 representative strains was done.
RESULTS:
Among hospitalized and OPD patients, 53.4% and 47.5% cases were positive for rotaviruses, respectively. Unlike previous studies where G1 was predominant, in hospitalized cases G9 rotavirus strains were most prevalent (40%), followed by G2 (39.6%) whereas G1 and G12 occurred at 16.4% and 5.6% frequency. In OPD cases, the most prevalent strain was G2 (40.3%), followed by G1, G9 and G12 at 25.5%, 22.8%, 9.3%, respectively. Phylogenetically the G1, G2 and G9 strains from Kolkata did not cluster with corresponding genotypes of Rotarix, RotaTeq and Rotavac (116E) vaccine strains.
CONCLUSION:
The study highlights the high prevalence of RV in children with gastroenteritis in Kolkata. The circulating genotypes have changed over the time with predominance of G9 and G2 strains during 2011-2013. The current G2, G9 and G1 Kolkata strains shared low amino acid homologies with current vaccine strains. Although there is substantial evidence for cross protection of vaccines against a variety of strains, still the strain variation should be monitored post vaccine introduction to determine if it has any impact on vaccine effectiveness.
en-copyright=
kn-copyright=
en-aut-name=MullickSatarupa
en-aut-sei=Mullick
en-aut-mei=Satarupa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MandalPaulami
en-aut-sei=Mandal
en-aut-mei=Paulami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Mukti Kant Nayak
en-aut-sei=Mukti Kant Nayak
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=GhoshSouvik
en-aut-sei=Ghosh
en-aut-mei=Souvik
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=DePapiya
en-aut-sei=De
en-aut-mei=Papiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=RajendranK.
en-aut-sei=Rajendran
en-aut-mei=K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=BhattacharyaMihir K.
en-aut-sei=Bhattacharya
en-aut-mei=Mihir K.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=MitraUtpala
en-aut-sei=Mitra
en-aut-mei=Utpala
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=RamamurthyThandavarayan
en-aut-sei=Ramamurthy
en-aut-mei=Thandavarayan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=KobayashiNobumichi
en-aut-sei=Kobayashi
en-aut-mei=Nobumichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=Chawla-SarkarMamta
en-aut-sei=Chawla-Sarkar
en-aut-mei=Mamta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
affil-num=1
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=2
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=3
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=4
en-affil=Department of Hygiene, Sapporo Medical University School of Medicine
kn-affil=
affil-num=5
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=6
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=7
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=8
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=9
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
affil-num=10
en-affil=Department of Hygiene, Sapporo Medical University School of Medicine
kn-affil=
affil-num=11
en-affil=National Institute of Cholera and Enteric Diseases
kn-affil=
en-keyword=Diarrhea
kn-keyword=Diarrhea
en-keyword=Rotavirus
kn-keyword=Rotavirus
en-keyword=India
kn-keyword=India
en-keyword=Kolkata
kn-keyword=Kolkata
en-keyword=G9 strains
kn-keyword=G9 strains
en-keyword=G2 strains
kn-keyword=G2 strains
END
start-ver=1.4
cd-journal=joma
no-vol=200
cd-vols=
no-issue=Part.A
article-no=
start-page=42
end-page=46
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A model-free method for extracting interaction potential between protein molecules using small-angle X-ray
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= A small-angle X-ray scattering has been used to probe protein–protein interaction in solution. Conventional methods need to input modeled potentials with variable/invariable parameters to reproduce the experimental structure factor. In the present study, a model-free method for extracting the excess part of effective interaction potential between protein molecules in solutions over an introduced hard-sphere potential by using experimental data of small-angle X-ray scattering is presented on the basis of liquid-state integral equation theory. The reliability of the model-free method is tested by the application to experimentally derived structure factors for dense lysozyme solutions with different solution conditions [Javid et al., Phys. Rev. Lett. 99, 028101 (2007), Schroer et al., Phys. Rev. Lett. 106, 178102 (2011)]. The structure factors calculated from the model-free method agree well with the experimental ones. The model-free method provides the following picture of the lysozyme solution: these are the stabilization of contact-pair configurations, large activation barrier against their formations, and screened Coulomb repulsion between the charged proteins. In addition, the model-free method will be useful to verify whether or not a model for colloidal system is acceptable to describing protein–protein interaction.
en-copyright=
kn-copyright=
en-aut-name=SumiTomonari
en-aut-sei=Sumi
en-aut-mei=Tomonari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=ImamuraHiroshi
en-aut-sei=Imamura
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MoritaTakeshi
en-aut-sei=Morita
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NishikawaKeiko
en-aut-sei=Nishikawa
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Department of Chemistry, Faculty of Science, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Advanced integration Science, Chiba University
kn-affil=
affil-num=3
en-affil=Graduate School of Advanced integration Science, Chiba University
kn-affil=
affil-num=4
en-affil=Graduate School of Advanced integration Science, Chiba University
kn-affil=
en-keyword=Protein solutions
kn-keyword=Protein solutions
en-keyword=Lysozyme
kn-keyword=Lysozyme
en-keyword=Protein-protein interaction
kn-keyword=Protein-protein interaction
en-keyword=Liquid state theory
kn-keyword=Liquid state theory
en-keyword=Integral equation
kn-keyword=Integral equation
en-keyword=DLVO model
kn-keyword=DLVO model
END
start-ver=1.4
cd-journal=joma
no-vol=177
cd-vols=
no-issue=1
article-no=
start-page=75
end-page=86
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201310
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Characterization of burdock mottle virus, a novel member of the genus Benyvirus, and the identification of benyvirus-related sequences in the plant and insect genomes.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= The complete nucleotide sequence of the burdock mottle virus (BdMoV) isolated from an edible burdock plant (Arctium lappa) in Japan has been determined. BdMoV has a bipartite genome, whose organization is similar to RNA1 and RNA2 of benyviruses, beet necrotic yellow vein virus (BNYVV), beet soil-borne mosaic virus (BSBMV), and rice stripe necrosis virus (RSNV). BdMoV RNA1 (7038 nt) contains a single open reading frame (ORF) encoding a 249-kDa polypeptide that consists of methyl-transferase, helicase, papain-like protease, AlkB-like, and RNA-dependent RNA polymerase domains. The AlkB-like domain sequence is not present in the proteins encoded by other known benyviruses, but is found in replication-associated proteins of viruses mainly belonging to the families Alfaflexiviridae and Betaflexiviridae. BdMoV RNA2 (4315 nt) contains six ORFs that are similar to those of benyviruses: these are coat protein (CP), CP readthrough, triple gene block movement and cysteine-rich proteins. Phylogenetic analyses showed that BdMoV is more closely related to BNYVV and BSBMV than to RSNV. Database searches showed that benyvirus replicase-related sequences are present in the chromosomes of a chickpea plant (Cicer arietinum) and a blood-sucking insect (Rhodnius prolixus). Some other benyvirus-related sequences are found in the transcriptome shotgun libraries of a few species of plants and a bark beetle. Our results show that BdMoV is a distinct species of the genus Benyvirus and that ancestral and extant benyviruses may have infected or currently infect a wide range of hosts, including plants and insects.
en-copyright=
kn-copyright=
en-aut-name=KondoHideki
en-aut-sei=Kondo
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HiranoShuichi
en-aut-sei=Hirano
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ChibaSotaro
en-aut-sei=Chiba
en-aut-mei=Sotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=AndikaIda Bagus
en-aut-sei=Andika
en-aut-mei=Ida Bagus
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=HiraiMakoto
en-aut-sei=Hirai
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MaedaTakanori
en-aut-sei=Maeda
en-aut-mei=Takanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=TamadaTetsuo
en-aut-sei=Tamada
en-aut-mei=Tetsuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=2
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=3
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
affil-num=4
en-affil=Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences
kn-affil=
affil-num=5
en-affil=Department of Parasitology, Graduate School of Medicine, Gunma University
kn-affil=
affil-num=6
en-affil=Formerly College of Bioresource Sciences, Nihon University
kn-affil=
affil-num=7
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
en-keyword=AlkB
kn-keyword=AlkB
en-keyword=Benyvirus
kn-keyword=Benyvirus
en-keyword=Burdock mottle virus
kn-keyword=Burdock mottle virus
en-keyword=Endogenous viral element
kn-keyword=Endogenous viral element
en-keyword=Paleovirology
kn-keyword=Paleovirology
en-keyword=Transcriptome shotgun assembly
kn-keyword=Transcriptome shotgun assembly
END
start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=1-2
article-no=
start-page=220
end-page=224
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=201307
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= The aim of this study was to assess the anticonvulsant effect of carbon dioxide (CO2) on Scn1a mutation-related febrile seizures. We examined physiological changes in the blood gas levels after the induction of hyperthermia-induced seizures (HISs), which were associated with the Scn1a missense mutation. We determined the efficacy of inhalation of 5% or 10% CO2 to treat HISs. HISs were evoked in Scn1a mutant and wild-type (WT) rats by hot water baths. To determine the anticonvulsant effect of CO2 inhalation, rats were placed in a chamber filled with air or mixed gas containing 5% CO2 or 10% CO2 for 3 min, immediately after the induction of HISs. We also analyzed the blood gas levels at the end of inhalation of CO2. Hot water bathing induced a significant reduction in the partial pressure of CO2 (pCO2) and respiratory alkalosis in the WT and Scn1a mutant rats. HISs were evoked in 100% of the Scn1a mutant rats within 5 min, but in none of the WT rats. The Scn1a mutant rats demonstrated a higher HISs susceptibility associated with respiratory alkalosis than the WT rats. Inhalation of 10% CO2 shortened the seizure duration from 62.6±12.1 s to 15.5±1.0 s. Blood gas analysis after the inhalation of 10% CO2 demonstrated an elevated pCO2 level and respiratory acidosis. Inhalation of 10% CO2 demonstrated a potent and fast-acting anticonvulsant effect against HISs.
en-copyright=
kn-copyright=
en-aut-name=OhmoriIori
en-aut-sei=Ohmori
en-aut-mei=Iori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HayashiKeiichiro
en-aut-sei=Hayashi
en-aut-mei=Keiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=WangHaijiao
en-aut-sei=Wang
en-aut-mei=Haijiao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=OuchidaMamoru
en-aut-sei=Ouchida
en-aut-mei=Mamoru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=FujitaNaohiro
en-aut-sei=Fujita
en-aut-mei=Naohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=InoueTakushi
en-aut-sei=Inoue
en-aut-mei=Takushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MichiueHiroyuki
en-aut-sei=Michiue
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=NishikiTeiichi
en-aut-sei=Nishiki
en-aut-mei=Teiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=MatsuiHideki
en-aut-sei=Matsui
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
affil-num=1
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Molecular Genetics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=6
en-affil=Department of Child Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=7
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=8
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=9
en-affil=Department of Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=167
cd-vols=
no-issue=1
article-no=
start-page=74
end-page=85
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201207
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Identification of host genes showing differential expression profiles with cell-based long-term replication of hepatitis C virus RNA
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Persistent hepatitis C virus (HCV) infection frequently causes hepatocellular carcinoma. However, the mechanisms of HCV-associated hepatocarcinogenesis and disease progression are unclear. Although the human hepatoma cell line, HuH-7, has been widely used as the only cell culture system for robust HCV replication, we recently developed new human hepatoma Li23 cell line-derived OL, OL8, OL11, and OL14 cells, in which genome-length HCV RNA (O strain of genotype 1b) efficiently replicates. OL, OL8, OL11, and OL14 cells were cultured for more than 2 years. We prepared cured cells from OL8 and OL11 cells by interferon-γ treatment. The cured cells were also cultured for more than 2 years. cDNA microarray and RT-PCR analyses were performed using total RNAs prepared from these cells. We first selected several hundred highly or moderately expressed probes, the expression levels of which were upregulated or downregulated at ratios of more than 2 or less than 0.5 in each set of compared cells (e.g., parent OL8 cells versus OL8 cells cultured for 2 years). From among these probes, we next selected those whose expression levels commonly changed during a 2-year culture of genome-length HCV RNA-replicating cells, but which did not change during a 2-year culture period in cured cells. We further examined the expression levels of the selected candidate genes by RT-PCR analysis using additional specimens from the cells cultured for 3.5 years. Reproducibility of the RT-PCR analysis using specimens from recultured cells was also confirmed. Finally, we identified 5 upregulated genes and 4 downregulated genes, the expression levels of which were irreversibly altered during 3.5-year replication of HCV RNA. These genes may play roles in the optimization of the environment in HCV RNA replication, or may play key roles in the progression of HCV-associated hepatic diseases.
en-copyright=
kn-copyright=
en-aut-name=SejimaHiroe
en-aut-sei=Sejima
en-aut-mei=Hiroe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MoriKyoko
en-aut-sei=Mori
en-aut-mei=Kyoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=AriumiYasuo
en-aut-sei=Ariumi
en-aut-mei=Yasuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=IkedaMasanori
en-aut-sei=Ikeda
en-aut-mei=Masanori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KatoNobuyuki
en-aut-sei=Kato
en-aut-mei=Nobuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
kn-affil=
en-keyword=HCV
kn-keyword=HCV
en-keyword=HCV RNA replication system
kn-keyword=HCV RNA replication system
en-keyword=Li23 cells
kn-keyword=Li23 cells
en-keyword=Long-term RNA replication
kn-keyword=Long-term RNA replication
en-keyword=Upregulated host genes
kn-keyword=Upregulated host genes
en-keyword=Downregulated host genes
kn-keyword=Downregulated host genes
END