start-ver=1.4
cd-journal=joma
no-vol=27
cd-vols=
no-issue=1
article-no=
start-page=181
end-page=189
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210210
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Medial meniscus posterior root repairs: A comparison among three surgical techniques in short-term clinical outcomes and arthroscopic meniscal healing scores
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background
Medial meniscus (MM) posterior root repairs lead to favorable clinical outcomes in patients with MM posterior root tears (MMPRTs). However, there are few comparative studies in evaluating the superiority among several pullout repair techniques such as modified Mason?Allen suture, simple stitch, and concomitant posteromedial pullout repair. We hypothesized that an additional pullout suture at the MM posteromedial part would have clinical advantages in transtibial pullout repairs of the MMPRTs. The aim of this study was to compare the clinical usefulness among several types of pullout repair techniques in patients with MMPRTs.
Methods
Eighty-three patients who underwent arthroscopic pullout repairs of the MMPRTs were investigated. Patients were divided into three groups using different pullout repair techniques: a modified Mason?Allen suture using FasT-Fix all-inside meniscal repair device (F-MMA, n?=?28), two simple stitches (TSS, n?=?30), and TSS concomitant with posteromedial pullout repair using all-inside meniscal repair device (TSS-PM, n?=?25). Postoperative clinical outcomes and semi-quantitative arthroscopic meniscal healing scores (0?10 points) were evaluated at second-look arthroscopies.
Results
No significant differences among the three groups were observed in patient demographics and preoperative clinical scores, except for preoperative Lysholm scores. At second-look arthroscopies, there were no significant differences among the three techniques in postoperative clinical outcomes and meniscal healing scores.
Conclusions
This study demonstrated that the TSS-PM pullout repair technique did not show better scores in postoperative clinical outcomes and meniscal healings compared with the F-MMA and TSS techniques. Our results suggest that the concomitant posteromedial pullout suture may have no clinical advantage in the conventional pullout repairs for the patients with MMPRTs.
en-copyright=
kn-copyright=
en-aut-name=FurumatsuTakayuki
en-aut-sei=Furumatsu
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HiranakaTakaaki
en-aut-sei=Hiranaka
en-aut-mei=Takaaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OkazakiYuki
en-aut-sei=Okazaki
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KintakaKeisuke
en-aut-sei=Kintaka
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KodamaYuya
en-aut-sei=Kodama
en-aut-mei=Yuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KamatsukiYusuke
en-aut-sei=Kamatsuki
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=OzakiToshifumi
en-aut-sei=Ozaki
en-aut-mei=Toshifumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
affil-num=2
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
affil-num=3
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
affil-num=4
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
affil-num=5
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
affil-num=6
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
affil-num=7
en-affil=Department of Orthopaedic Surgery, Okayama University Hospital
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=
article-no=
start-page=41
end-page=44
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190731
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A proposed simple screening method to determine relative contributions of CYP3A4 and CYP3A5 to drug metabolism in?vitro
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Purpose
The cytochrome P450 (CYP) 3A family of enzymes metabolize the majority of clinically used drugs. CYP3A4 and CYP3A5 are the two major CYP3A isoforms, but exhinbit different substrate specificity. The aim of this study was to establish a simple screening method to determine the relative contributions of CYP3A4 and CYP3A5 to drug metabolism in vitro.
Methods
A screening method was developed based on competitive inhibition using luciferin-PPXE (L-PPXE), a luminogenic CYP3A substrate. CYP3cide, tacrolimus, and midazolam were selected as standard compounds metabolized by CYP3A4 or CYP3A5. Nine clinically-used drugs were evaluated for their abilities to inhibit luminescence resulting from L-PPXE metabolism. Appropriate reaction conditions for the screening method were determined using recombinant CYP3A4 and CYP3A5.
Results
A significant decrease in luminescence resulting from L-PPXE metabolism by CYP3A4 and CYP3A5 was observed only for drugs reported to be metabolized by CYP3As. The substrate specificities of CYP3A4 or CYP3A5 for the proposed CYP3A substrates using our screening method were consistent with those of previous reports or available drug information from pharmaceutical companies. The reaction volume for this method was 50 ƒÊL, and the time required for the entire procedure was 70 min. Furthermore, this screening can be performed using a single tube with minimal training.
Conclusions
Through the establishment of our screening method in the present study, we are sure it is useful to determine the relative contributions of CYP3A4 and CYP3A5 to drug metabolism in vitro.
en-copyright=
kn-copyright=
en-aut-name=MatsumotoJun
en-aut-sei=Matsumoto
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=NakamuraHiroyoshi
en-aut-sei=Nakamura
en-aut-mei=Hiroyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=SanSu Nwe
en-aut-sei=San
en-aut-mei=Su Nwe
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=SatoHikari
en-aut-sei=Sato
en-aut-mei=Hikari
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=TakezawaManami
en-aut-sei=Takezawa
en-aut-mei=Manami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KishiRyuto
en-aut-sei=Kishi
en-aut-mei=Ryuto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=KitoYutaro
en-aut-sei=Kito
en-aut-mei=Yutaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=SuganoJunko
en-aut-sei=Sugano
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=IzukiMai
en-aut-sei=Izuki
en-aut-mei=Mai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=YanagisawaNao
en-aut-sei=Yanagisawa
en-aut-mei=Nao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=IkedaNaoki
en-aut-sei=Ikeda
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=SaitoYusuke
en-aut-sei=Saito
en-aut-mei=Yusuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=KatoYoshinori
en-aut-sei=Kato
en-aut-mei=Yoshinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=YamadaHarumi
en-aut-sei=Yamada
en-aut-mei=Harumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
en-aut-name=FujiyoshiMasachika
en-aut-sei=Fujiyoshi
en-aut-mei=Masachika
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=
en-aut-name=AriyoshiNoritaka
en-aut-sei=Ariyoshi
en-aut-mei=Noritaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=
affil-num=1
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=3
en-affil=Graduate School of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=4
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=5
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=6
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=7
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=8
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=9
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=10
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=11
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=12
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=13
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=14
en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare
kn-affil=
affil-num=15
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=16
en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
en-keyword=CYP3A
kn-keyword=CYP3A
en-keyword=CYP3A5*3 allele
kn-keyword=CYP3A5*3 allele
en-keyword=P450 Glo Assay system
kn-keyword=P450 Glo Assay system
en-keyword=Pharmacokinetics
kn-keyword=Pharmacokinetics
en-keyword=Pharmacogenetics
kn-keyword=Pharmacogenetics
END
start-ver=1.4
cd-journal=joma
no-vol=924
cd-vols=
no-issue=
article-no=
start-page=60
end-page=67
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160614
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Chelate titrations of Ca2+ and Mg2+ using microfluidic paper-based analytical devices
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We developed microfluidic paper-based analytical devices (ƒÊPADs) for the chelate titrations of Ca2+ and Mg2+ in natural water. The ƒÊPAD consisted of ten reaction zones and ten detection zones connected through narrow channels to a sample zone located at the center. Buffer solutions with a pH of 10 or 13 were applied to all surfaces of the channels and zones. Different amounts of ethylenediaminetetraacetic acid (EDTA) were added to the reaction zones and a consistent amount of a metal indicator (Eriochrome Black T or Calcon) was added to the detection zones. The total concentrations of Ca2+ and Mg2+ (total hardness) in the water were measured using a ƒÊPAD containing a buffer solution with a pH of 10, whereas only Ca2+ was titrated using a ƒÊPAD prepared with a potassium hydroxide solution with a pH of 13. The ƒÊPADs permitted the determination of Ca2+ and Mg2+ in mineral water, river water, and seawater samples within only a few minutes using only the naked eye?no need of instruments.
en-copyright=
kn-copyright=
en-aut-name=KaritaShingo
en-aut-sei=Karita
en-aut-mei=Shingo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
affil-num=1
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=‰ªŽR‘åŠw‘åŠw‰@Ž©‘R‰ÈŠwŒ¤‹†‰È
affil-num=2
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=‰ªŽR‘åŠw‘åŠw‰@Ž©‘R‰ÈŠwŒ¤‹†‰È
en-keyword=Chelate titration
kn-keyword=Chelate titration
en-keyword=Microfluidic paper-based analytical device
kn-keyword=Microfluidic paper-based analytical device
en-keyword=Hardness
kn-keyword=Hardness
en-keyword=Calcium
kn-keyword=Calcium
en-keyword=Magnesium
kn-keyword=Magnesium
en-keyword=Natural water
kn-keyword=Natural water
END