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ID 48823
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Author
Koreishi, Mayuko
Abstract
Gene perturbation methods are commonly used in the study of gene and protein function. The authors of this paper recently developed a rapid protein inactivation technique utilizing tobacco etch virus (TEV)-derived protease. TEV protease recognizes the ENLYFQG (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) amino acid sequence and specifically cleaves between Q and G. The authors developed antibodies that recognize the cleaved TEV (ENLYFQ) sequence, both in vitro and in vivo, but do not bind to uncleaved TEV (ENLYFQG). Using these antibodies, in situ protein cleavage was successfully detected. These antibodies used in combination with the TEV protease may be a useful complement to other perturbation methods.
Keywords
TEV protease
Golgi
golgins
microinjection
recombinant proteins
Published Date
201-08-09
Publication Title
Antibody Technology Journal
Volume
volume2011
Issue
issue1
Publisher
Dove Medical Press
Start Page
5
End Page
11
ISSN
2230-3170
Content Type
Journal Article
Official Url
http://www.dovepress.com/detection-of-in-situ-cleaved-p115-with-the-cut-specific-antibodies-in--peer-reviewed-article-ANTI
language
英語
Copyright Holders
© 2011 Koreishi et al
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publisher
Refereed
True
DOI