このエントリーをはてなブックマークに追加
ID 53002
FullText URL
Author
Moritou, Yuki
Takeuchi, Yasuto
Seki, Hiroyuki
Nanba, Shintaro
Abstract
We encountered a patient positive for anti-hepatitis C virus (HCV) whose serum HCV RNA was undetectable with the Roche AmpliPrep/Cobas TaqMan HCV assay (CAP/CTM) version 1 but showed a high viral load with the Abbott RealTime HCV assay (ART). Discrepancies in the detectability of serum HCV RNA were investigated among 891 consecutive patients who were positive for anti-HCV. Specific nucleotide variations causing the undetectability of HCV RNA were determined and confirmed by synthesizing RNA coding those variations. Serum samples with the discrepancies were also reassessed by CAP/CTM version 2. Among the 891 anti-HCV-positive patients, 4 patients had serum HCV RNA levels that were undetectable by CAP/CTM version 1 despite having levels of > 5 log IU/ml that were detected by ART. All four patients had HCV genotype 2a and high titers of anti-HCV. Sequencing of the HCV 5' noncoding regions revealed 2 common variations, A at nucleotide (nt) 145 and T at nt 151. Synthesized RNAs of the HCV 5' noncoding region with standard (NCR145G151C) and variant nucleotides at nt 145 and nt 151 were quantified with CAP/CTM. RNAs of NCR145G151C and NCR145G151T were quantifiable with CAP/CTM version 1, while those of NCR145A151T and NCR145A151C went undetected. The substitution from G to A at nt 145 specifically conferred this undetectability, while this undetectability was reverted in synthesized HCV RNA with correction of this variation. Reassessment of these samples by CAP/CTM version 2 resulted in similar levels of HCV RNA being detected by ART. We conclude that HCV patients with undetectable HCV RNA by CAP/CTM version 1 should be reassessed for viral quantification.
Published Date
2014-02
Publication Title
Journal of Clinical Microbiology
Volume
volume52
Issue
issue2
Publisher
American Society for Microbiology
Start Page
544
End Page
548
ISSN
0095-1137
NCID
AA00695531
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52957
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
File Version
author
Refereed
True
DOI
Web of Sience KeyUT