ID | 46906 |
FullText URL | |
Author |
Saiga, Kenta
Yoshida, Aki
Masuda, Shin
Takihira, Shota
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Abstract | Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.
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Keywords | Medial collateral ligament
bFGF
GDF-5
Hydrogel scaffold
Ligament healing
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Published Date | 2010-11-12
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Publication Title |
Biochemical and Biophysical Research Communications
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Volume | volume402
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Issue | issue2
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Publisher | Academic Press Inc Elsevier Science
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Start Page | 329
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End Page | 334
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ISSN | 0006-291X
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NCID | AA00564395
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Content Type |
Journal Article
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language |
English
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Copyright Holders | © 2010 Elsevier Inc. All rights reserved.
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File Version | author
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Refereed |
True
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DOI | |
PubMed ID | |
Web of Science KeyUT |