start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=27
end-page=38
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2024.
kn-title=公表学術論文等リスト　2024
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=21
end-page=25
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Elucidation of plant-bacterial pathogen interactions for the control of bacterial blight on cruciferous crops
kn-title=アブラナ科植物黒斑細菌病の防除に向けた植物－病原細菌の相互作用の解明
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Pseudomonas cannabina pv. alisalensis (Pcal), the causative agent of bacterial blight on cruciferous crops, is an economically important pathogen worldwide. We have conducted several studies on the interactions between plants and pathogenic bacteria to develop effective control strategies for this disease. Using forward and reverse genetics, we identified several virulence factors, including the type III secretion system, membrane transporters, transcriptional factors, and amino acid metabolism. Additionally, we emphasized the role of coronatine, a toxin produced by Pcal, which promotes stomatal reopening and suppresses salicylic acid accumulation in plants. We also examined plant defense mechanisms activated by one of the plant defense activators, acibenzolar-S-methyl (ASM). ASM enhanced stomatal-based defense, resulting in reduction of bacterial entry and disease development. Moreover, we explored innovative control strategies for bacterial disease and demonstrated that amino acids and cellulose nanofiber are efficient and environmentally friendly control strategies. These studies advance our understanding of plant-pathogen dynamics and offer promising, sustainable approaches for managing bacterial blight disease in cruciferous crops.
en-copyright=
kn-copyright=

en-aut-name=SakataNanami
en-aut-sei=Sakata
en-aut-mei=Nanami
kn-aut-name=坂田七海
kn-aut-sei=坂田
kn-aut-mei=七海
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Course of Applied Plant Science
kn-affil=応用植物科学コース
en-keyword=Plant pathogenic bacteria
kn-keyword=Plant pathogenic bacteria
en-keyword=Pseudomonas
kn-keyword=Pseudomonas
en-keyword=Cruciferous
kn-keyword=Cruciferous
en-keyword=Plant protection
kn-keyword=Plant protection
en-keyword=Stomata
kn-keyword=Stomata
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=11
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Uncovering the role of arcuate kisspeptin neurons as a source of the gonadotropin-releasing hormone pulse generator using gene-modified rats
kn-title=遺伝子改変ラットを用いた弓状核キスペプチンニューロンの性腺刺激ホルモン放出ホルモンパルスジェネレーターとしての役割解明
en-subtitle=
kn-subtitle=
en-abstract=　世界において，乳牛の受胎率（妊娠率）が低下しており，家畜の繁殖成績向上のための効果的な治療法が必要とされている．家畜を含む哺乳類において，生殖機能は視床下部－下垂体－性腺軸から分泌されるホルモンによって制御されている．これらのホルモンのうち，性腺刺激ホルモン放出ホルモン（GnRH）のパルス状分泌（GnRH パルス）は，性腺刺激ホルモン分泌と性腺機能に本質的に重要である．したがって，GnRH パルスを制御するメカニズム（GnRH パルスジェネレーター）を解明することは，家畜の生殖技術を向上させるために不可欠である．本総説では，著者らの遺伝子改変ラットモデルを用いた弓状核キスペプチンニューロン（ΚNDy ニューロンとしても知られる）がGnRH パルスジェネレーターの本体であることの直接的な証拠を示した研究を中心として，過去20年間の研究を概説した．また，ΚNDy ニューロンが分泌するニューロキニンB，グルタミン酸，ダイノルフィンA がΚNDy ニューロンの神経活動を同期させ，GnRH パルスを発生させるメカニズムについて論じた．遺伝子改変ラットモデルから得られた知識は，GnRH/ 性腺刺激ホルモンパルスを刺激して，家畜の繁殖能力を向上させる新規繁殖促進剤開発に寄与すると期待できる．
kn-abstract=　Strategies for increasing reproductive performance are needed for domestic animals because for example the conception (pregnancy) rate has decreased in dairy cows around the world. Reproductive function is controlled by hormones released by the hypothalamus-pituitary-gonadal axis in mammals, including domestic animals. Of those hormones, tonic (pulsatile) gonadotropin-releasing hormone (GnRH) release is fundamentally important for gonadotropin release and gonadal activity. Therefore, uncovering the mechanism controlling GnRH pulses, that is GnRH pulse generator, is essential to improve reproductive technologies for domestic animals. The present review is focused on the indispensable role of arcuate nucleus (ARC) kisspeptin neurons (also known as KNDy neurons) as the GnRH pulse generator in mammals. First, we give a brief overview of studies on hypothalamic kisspeptin neurons throughout the past two decades. Second, we review studies that have provided direct evidence that ARC kisspeptin neurons serve as the GnRH pulse generator, with a special focus on our gene-modified rat models. Finally, we discuss the mechanism underlying GnRH pulse generation. The knowledge obtained from gene-modified rat models should be clinically important and could be adapted to new tools to improve reproductive performance in livestock by stimulating GnRH/gonadotropin pulses.
en-copyright=
kn-copyright=

en-aut-name=NagaeMayuko
en-aut-sei=Nagae
en-aut-mei=Mayuko
kn-aut-name=長江麻佑子
kn-aut-sei=長江
kn-aut-mei=麻佑子
aut-affil-num=1
ORCID=

en-aut-name=UenoyamaYoshihisa
en-aut-sei=Uenoyama
en-aut-mei=Yoshihisa
kn-aut-name=上野山賀久
kn-aut-sei=上野山
kn-aut-mei=賀久
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院環境生命自然科学研究科

affil-num=2
en-affil=Graduate School of Bioagricultural Sciences, Nagoya University
kn-affil=名古屋大学大学院生命農学研究科
en-keyword=gene-modified rats
kn-keyword=gene-modified rats
en-keyword=GnRH
kn-keyword=GnRH
en-keyword=kisspeptin
kn-keyword=kisspeptin
en-keyword=LH
kn-keyword=LH
en-keyword=pulse generator
kn-keyword=pulse generator
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=1
end-page=10
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of dark respiration on dry matter production of various crop species
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Eleven crops were cultivated: maize, sunflower, soybean, groundnuts, sesame, kenaf, barley, wheat, rice, potato, and sweet potato. The crop growth rate (CGR) and specific dark-respiration rate (Rs) were measured, and growth efficiency GE =CGR/(CGR+R) (R, respiratory loss) was calculated. In each crop, whole-plant Rs reached a maximum in the earlier stages of growth, declined rapidly until the early reproductive growth, and remained almost constant during the ripening period. The Rs of leaves was higher than that of stems during the reproductive growth period, except for maize and potato. The Rs of storage organs was highest in the earlier stages, followed by a rapid decline to similar or lower values than those of leaves and stems during the ripening period. The GE in whole plant was higher than 60% in wheat, maize, barley, sunflower, rice, kenaf, sesame, but lower in soybean, sweet potato and groundnuts, and lowest in potato, which was affected by the higher respiratory loss. The GE in whole plant during the reproductive growth period was significantly lower, which we attributed to increased maintenance costs due to the increase of non-assimilative organs, and decrease in the dry weight of vegetative organs. A positive correlation was observed between the carbohydrate content of storage organs and GE, indicating that a crop with higher carbohydrate content in storage organs tended to have a higher GE. Crops with higher protein and crude fat content in storage organs tended to have lower GE. The GE over the growing season was low for kenaf, a fiber crop which contains high molecular weight compounds such as lignin and cellulose, and lower for sesame, groundnuts, and soybean, which contain high oil and protein and have high respiration costs for the synthesis of storage materials, suggesting that these higher respiration costs are related to lower dry matter production and hence lower yields.
en-copyright=
kn-copyright=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MurakamiTomohiro
en-aut-sei=Murakami
en-aut-mei=Tomohiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NakamuraYumi
en-aut-sei=Nakamura
en-aut-mei=Yumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NishiboriMisa
en-aut-sei=Nishibori
en-aut-mei=Misa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakagoshiYuki
en-aut-sei=Takagoshi
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=HiraiYoshihiko
en-aut-sei=Hirai
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=School of Agriculture, Okayama University
kn-affil=

affil-num=4
en-affil=School of Agriculture, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Cereal crops
kn-keyword=Cereal crops
en-keyword=Oil crops
kn-keyword=Oil crops
en-keyword=Crop growth rate
kn-keyword=Crop growth rate
en-keyword=Dark-respiration
kn-keyword=Dark-respiration
en-keyword=Growth efficiency
kn-keyword=Growth efficiency
en-keyword=Leguminous crops
kn-keyword=Leguminous crops
en-keyword=Nutrients composition
kn-keyword=Nutrients composition
en-keyword=Respiratory loss
kn-keyword=Respiratory loss
en-keyword=Root and tuber crops
kn-keyword=Root and tuber crops
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=114
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2025
dt-pub=20250201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=61
end-page=73
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2023.
kn-title=公表学術論文等リスト　2023
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=55
end-page=59
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Application of grafting-induced flowering to the breeding and seed production of cruciferous crops
kn-title=接ぎ木による開花誘導のアブラナ科作物の育種および採種への利用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Grafting-induced flowering has the potential to be applied to plant breeding and seed production, to shorten the time needed for floral induction, and to expand the environment in which seed production is possible. However, it is difficult to induce flowering by grafting in some crop species, which currently limits the use of this technique to specific plant species. We have established a technique for inducing flowering in cabbage (Brassica oleracea L.) by grafting, which had hitherto been considered difficult, by clarifying the causes of different flowering responses caused by various rootstocks. This article introduces the key factors for the floral induction of cabbage by grafting and its potential application to the breeding and seed production of cruciferous crops. It was observed that cabbage grafted onto B. oleracea rootstocks did not flower at all, while cabbage grafted onto some accessions of Raphanus sativus rootstocks did flower. Furthermore, the ability of R. sativus to induce flowering of grafted cabbage varied even within the species. Immunoblotting analysis of the FLOWERING LOCUS T (FT) protein, a main component of florigen, revealed that floral induction was quantitatively correlated with the level of accumulated FT protein in the grafted scion. It was concluded that increasing the total amount of FT protein produced in the rootstock is important for the stable floral induction of the grafted cabbage, and this can be accomplished by increasing FT transcription and the leaf area of the rootstock. Field cultivation experiments with cabbage progenies obtained by the grafting method indicated the direct applicability of this method to breeding and seed production in cruciferous crops.
en-copyright=
kn-copyright=

en-aut-name=MotokiKo
en-aut-sei=Motoki
en-aut-mei=Ko
kn-aut-name=元木航
kn-aut-sei=元木
kn-aut-mei=航
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Course of Applied Plant Science
kn-affil=応用植物科学コース
en-keyword=Brassica oleracea
kn-keyword=Brassica oleracea
en-keyword=Raphanus sativus
kn-keyword=Raphanus sativus
en-keyword=grafting
kn-keyword=grafting
en-keyword=rapid flowering induction
kn-keyword=rapid flowering induction
en-keyword=florigen
kn-keyword=florigen
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=49
end-page=54
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Identification of novel early flowering genes in tetraploid wheat (Triticum turgidum L.) and development of efficient methods for their genetic analysis
kn-title=四倍体コムギが保有する新規早生遺伝子の探索およびその効率的遺伝解析法の開発
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Wheat (Triticum spp.) is one of the world's three major cereals and a staple food for more than 35% of the world's population, and while nearly doubling crop yields is essential to meet the caloric demands of a growing population by 2050, current rates of wheat yield growth are insufficient to meet this demand. Therefore, it is important to promote the improvement of wheat varieties and to increase yields in a stable manner by utilizing all available genetic resources. Here I would like to introduce some research that has been conducted in this regard, using tetraploid wheat (Triticum turgidum L.) as a genetic resource in the search for genes related to flowering time and the research related to the streamlined genetic analysis technique.
en-copyright=
kn-copyright=

en-aut-name=NishimuraKazusa
en-aut-sei=Nishimura
en-aut-mei=Kazusa
kn-aut-name=西村和紗
kn-aut-sei=西村
kn-aut-mei=和紗
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Course of Applied Plant Science
kn-affil=応用植物科学コース
en-keyword=Wheat
kn-keyword=Wheat
en-keyword=Tetraploid wheat
kn-keyword=Tetraploid wheat
en-keyword=Flowering time
kn-keyword=Flowering time
en-keyword=Allopolyploid
kn-keyword=Allopolyploid
en-keyword=MIG-seq
kn-keyword=MIG-seq
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=41
end-page=48
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Instant estimation of rice yield using ground-based RGB images and its potential applicability to UAV
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Rice (Oryza sativa L.) is one of the most important cereals, which provides 20% of the world’s food energy. However, its productivity is poorly assessed especially in the global South. Here, we provide a first study to perform a deep learning-based approach for instantaneously estimating rice yield using RGB images. During ripening stage and at harvest, over 22,000 digital images were captured vertically downwards over the rice canopy from a distance of 0.8 to 0.9m at 4,820 harvesting plots having the yield of 0.1 to 16.1 t ha-1 across six countries in Africa and Japan. A convolutional neural network (CNN) applied to these data at harvest predicted 68% variation in yield with a relative root mean square error (rRMSE) of 0.22. Even when the resolution of images was reduced (from 0.2 to 3.2cm pixel-1 of ground sampling distance), the model could predict 57% variation in yield, implying that this approach can be scaled by use of unmanned aerial vehicles. Our work offers low-cost, hands-on, and rapid approach for high throughput phenotyping, and can lead to impact assessment of productivity-enhancing interventions, detection of fields where these are needed to sustainably increase crop production.
en-copyright=
kn-copyright=

en-aut-name=TanakaYu
en-aut-sei=Tanaka
en-aut-mei=Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WatanabeTomoya
en-aut-sei=Watanabe
en-aut-mei=Tomoya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KatsuraKeisuke
en-aut-sei=Katsura
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TsujimotoYasuhiro
en-aut-sei=Tsujimoto
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakaiToshiyuki
en-aut-sei=Takai
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanakaTakashi Sonam Tashi
en-aut-sei=Tanaka
en-aut-mei=Takashi Sonam Tashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KawamuraKensuke
en-aut-sei=Kawamura
en-aut-mei=Kensuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=SaitoHiroki
en-aut-sei=Saito
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HommaKoki
en-aut-sei=Homma
en-aut-mei=Koki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MairouaSalifou Goube
en-aut-sei=Mairoua
en-aut-mei=Salifou Goube
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=AhouantonKokou
en-aut-sei=Ahouanton
en-aut-mei=Kokou
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

en-aut-name=IbrahimAli
en-aut-sei=Ibrahim
en-aut-mei=Ali
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=

en-aut-name=SenthilkumarKalimuthu
en-aut-sei=Senthilkumar
en-aut-mei=Kalimuthu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=

en-aut-name=SemwalVimal Kumar
en-aut-sei=Semwal
en-aut-mei=Vimal Kumar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=

en-aut-name=MatuteEduardo Jose Graterol
en-aut-sei=Matute
en-aut-mei=Eduardo Jose Graterol
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=

en-aut-name=CorredorEdgar
en-aut-sei=Corredor
en-aut-mei=Edgar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=

en-aut-name=El-NamakyRaafat
en-aut-sei=El-Namaky
en-aut-mei=Raafat
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=17
ORCID=

en-aut-name=ManigbasNorvie
en-aut-sei=Manigbas
en-aut-mei=Norvie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=18
ORCID=

en-aut-name=QuilangEduardo Jimmy P.
en-aut-sei=Quilang
en-aut-mei=Eduardo Jimmy P.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=19
ORCID=

en-aut-name=IwahashiYu
en-aut-sei=Iwahashi
en-aut-mei=Yu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=20
ORCID=

en-aut-name=NakajimaKota
en-aut-sei=Nakajima
en-aut-mei=Kota
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=21
ORCID=

en-aut-name=TakeuchiEisuke
en-aut-sei=Takeuchi
en-aut-mei=Eisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=22
ORCID=

en-aut-name=SaitoKazuki
en-aut-sei=Saito
en-aut-mei=Kazuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=23
ORCID=

affil-num=1
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Mathematics, Kyushu University
kn-affil=

affil-num=3
en-affil=Graduate School of Agriculture, Tokyo University of Agriculture and Technology
kn-affil=

affil-num=4
en-affil=Japan International Research Center for Agricultural Sciences
kn-affil=

affil-num=5
en-affil=Japan International Research Center for Agricultural Sciences
kn-affil=

affil-num=6
en-affil=Faculty of Biological Sciences, Gifu UniversityFaculty of Biological Sciences, Gifu University
kn-affil=

affil-num=7
en-affil=Japan International Research Center for Agricultural Sciences
kn-affil=

affil-num=8
en-affil=Tropical Agriculture Research Front, Japan International Research Center for Agricultural Sciences
kn-affil=

affil-num=9
en-affil=Graduate School of Agricultural Science, Tohoku University
kn-affil=

affil-num=10
en-affil=Africa Rice Center （AfricaRice）
kn-affil=

affil-num=11
en-affil=Africa Rice Center （AfricaRice）
kn-affil=

affil-num=12
en-affil=Africa Rice Center （AfricaRice）, Regional Station for the Sahel
kn-affil=

affil-num=13
en-affil=Africa Rice Center （AfricaRice）
kn-affil=

affil-num=14
en-affil=Africa Rice Center （AfricaRice）, Nigeria Station
kn-affil=

affil-num=15
en-affil=Latin American Fund for Irrigated Rice - The Alliance of Bioversity International and CIAT
kn-affil=

affil-num=16
en-affil=Latin American Fund for Irrigated Rice - The Alliance of Bioversity International and CIAT
kn-affil=

affil-num=17
en-affil=Rice Research and Training Center, Field Crops Research Institute
kn-affil=

affil-num=18
en-affil=Philippine Rice Research Institute （PhilRice）
kn-affil=

affil-num=19
en-affil=Philippine Rice Research Institute （PhilRice）
kn-affil=

affil-num=20
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=

affil-num=21
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=

affil-num=22
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=

affil-num=23
en-affil=Japan International Research Center for Agricultural Sciences
kn-affil=
en-keyword=Rice (Oryza sativa L.)
kn-keyword=Rice (Oryza sativa L.)
en-keyword=rough grain yield
kn-keyword=rough grain yield
en-keyword=convolutional neural network
kn-keyword=convolutional neural network
en-keyword=RGB images
kn-keyword=RGB images
en-keyword=UAV
kn-keyword=UAV
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=33
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Comparisons of nitrogen use efficiency between rice cv. Nipponbare and Takanari at different fertilization levels
kn-title=異なる施肥条件下における水稲品種の窒素利用効率 ―日本晴とタカナリの比較―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The rice cultivar Nipponbare and the high-yielding cultivar Takanari were cultivated in field trials for three years from 2003, and in pot trials (1/2,000a) for two years from 2004. In the field trials, the following three levels of fertilizer were applied：“0N” without fertilizer, “1N” with the standard amount(8kgN 10a–1), and “2N” with twice the standard amount (16kgN 10a–1). In the pot trial, three levels of fertilizer were applied：“0N” without fertilizer, “1.5N” with 1.5times the standard amount (1.5gN pot–1), and “3N” with three times the standard amount (3gN pot–1). In the field trials, yields were higher in Takanari (538 to 843g m–2) than in Nihonbare (423 to 577g m–2), and the increase in yield with fertilizer application was also larger in Takanari. This was related to the larger sink capacity of Takanari and the smaller decrease in the percentage of filled grain with larger sink capacity. The dry matter weight and nitrogen uptake at the panicle initiation stage were higher in the plots with higher fertilizer application, but the differences between the cultivars were small. Dry matter weight and nitrogen uptake at harvest time were higher in Takanari, and nitrogen use efficiency and sink production efficiency were also higher in Takanari, but the differences in nitrogen use efficiency between cultivars became smaller with increasing fertilizer application. The nitrogen use efficiency for dry matter production also decreased with increasing fertilizer application, and was higher in 2005 in Takanari. The leaf photosynthetic rate of Takanari was higher than that of Nipponbare in the pot experiment. The difference in leaf photosynthetic rate was related to the nitrogen use efficiency (photosynthetic rate / leaf nitrogen content), and the difference in leaf nitrogen content between cultivars was small. The nitrogen use efficiency for dry matter was highest in the “0N” and decreased with increasing fertilizer application, and was higher in Takanari than in Nipponbare. This was presumably related to the higher nitrogen use efficiency of photosynthesis. It was found that fertilizer application decreased nitrogen use efficiency and sink production efficiency, but yield increased with increasing sink capacity, and that differences in nitrogen use efficiency among cultivars were related to the amount of nitrogen absorbed up to the panicle initiation stage and sink production efficiency. In order to improve the efficiency of fertilizer application, it is desirable to increase nitrogen absorption, which is expressed as multiplying the number of days to panicle initiation and the rate of nitrogen absorption, and to select cultivars with higher sink production efficiency.
en-copyright=
kn-copyright=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=1
ORCID=

en-aut-name=DannoYusuke
en-aut-sei=Danno
en-aut-mei=Yusuke
kn-aut-name=檀野祐亮
kn-aut-sei=檀野
kn-aut-mei=祐亮
aut-affil-num=2
ORCID=

affil-num=1
en-affil=The Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院環境生命自然科学研究科

affil-num=2
en-affil=The Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科
en-keyword=High-yielding rice cultivar
kn-keyword=High-yielding rice cultivar
en-keyword=Nitrogen use efficiency
kn-keyword=Nitrogen use efficiency
en-keyword=Nitrogen uptake
kn-keyword=Nitrogen uptake
en-keyword=Sink capacity
kn-keyword=Sink capacity
en-keyword=Sink production efficiency
kn-keyword=Sink production efficiency
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=25
end-page=32
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=アメリカ産ダイズ品種‘UA4805’ の多収性に関する解析的研究－日本品種 ‘あきまろ’ との比較－
kn-title=Analytical studies on high-yielding characteristics of US soybean cv. ‘UA4805’ in comparison with Japanese cv. ‘Akimaro’
en-subtitle=
kn-subtitle=
en-abstract=2020年と2021年に岡山大学農学部附属山陽圏フィールド科学センター（34°41ʼN, 133°55ʼE）でダイズの栽培試験を行った．アメリカ品種‘UA4805’ と日本品種‘あきまろ’ の2 品種を供試し，栽植密度を12.5 株 m-2（疎植，80 × 10 cm）と25 株m-2（密植，80 × 5 cm）の2 段階として，2020年5 月25日（早期）， 6 月29日（普通期）， 8 月3 日（晩期）に播種した．2021年は畦幅80 cm（広畦）と30 cm（狭畦），栽植密度12.5株m-2と25株m-2の2 段階で栽培した．子実収量は，2020年と2021年ともに‘あきまろ’ よりも‘UA4805’ の方が高かった．播種時期が遅いほど，粒/茎比が高くなった．両品種ともに疎植区に比べ密植区で乾物重が大きくなった．‘あきまろ’ は‘UA4805’ よりも乾物重は大きかったが，子実収量は低かった．一方，ʻUA4805ʼ は乾物重が小さかったが，節数，莢数，子実数が多く，子実収量が高かった．‘あきまろ’ は特に密植区において，倒伏程度が大きかった．粒/茎比は，いずれの試験区においてもʻ あきまろ’に比べ‘UA4805’ が著しく高かった．結莢率は‘UA4805’ が‘あきまろ’ より2 倍近く高かった．‘UA4805’ の子実収量が‘あきまろ’ に比べて高かったのは，結莢率，粒/茎比が高く，倒伏程度が小さかったことによるが，乾物生産は‘あきまろ’ の方が大きかった．晩期栽培の場合，子実収量を向上させるためには栽植密度を高くすることが推奨された．ダイズの子実収量を向上させるには，狭畦栽培が効果的であった．
kn-abstract=Field experiments were conducted in 2020 and 2021 at the Field Science Center of Okayama Univ. (34°41’ N, 133°55’ E). Two Soybean cultivars ‘UA4805’ and ‘Akimaro’ were sown with two planting densities, 12.5plants m−2 (sparse, 80×10cm) and 25plants m−2 (dense, 80×5cm)on May 25 (early), June 29 (normal), and Aug. 3 (late) in 2020, and 80 and 30cm row-width, and 12.5 and 25 plant m−2 in 2021 on June 23. Seed yield was higher in ‘UA4805’ than in ‘Akimaro’ in 2020 and 2021. The later the sowing time, the higher the seeds/stem ratio. Both cultivars showed higher dry matter in dense planting. Dry matter was higher in ‘Akimaro’, while seed yield was lower than ‘UA4805’. In contrast, ‘UA4805’ showed lower dry matter with higher seed yield. The numbers of nodes, pods, and seeds were higher in ‘UA4805’ resulting in the higher seed yield. Lodging score is larger in ‘Akimaro’ especially in dense planting. The seeds/stem ratio is much higher in ‘UA4805’ than ‘Akimaro’ across 2 densities, 3 sowing times and 2 row width. Pods setting ratio was nearly two times higher in ‘UA4805’ compared to ‘Akimaro’. The greater seed yield of ‘UA4805’ compared to ‘Akimaro’ was due to the higher pod setting ratio, seeds/stem ratio, and lower lodging score, nevertheless the dry matter was larger in ‘Akimaro’. If late sowing is applied, higher planting density is recommended for better seed yield. Narrow row is an effective way to improve seed yield in soybean.
en-copyright=
kn-copyright=

en-aut-name=MaroufSultanzada Mohammad
en-aut-sei=Marouf
en-aut-mei=Sultanzada Mohammad
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HasegawaYu
en-aut-sei=Hasegawa
en-aut-mei=Yu
kn-aut-name=長谷川湧
kn-aut-sei=長谷川
kn-aut-mei=湧
aut-affil-num=2
ORCID=

en-aut-name=ManabeRyuta
en-aut-sei=Manabe
en-aut-mei=Ryuta
kn-aut-name=眞鍋竜太
kn-aut-sei=眞鍋
kn-aut-mei=竜太
aut-affil-num=3
ORCID=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=School of Agriculture
kn-affil=岡山大学農学部

affil-num=3
en-affil=School of Agriculture
kn-affil=岡山大学農学部

affil-num=4
en-affil=Graduate School of Environmental and Life Science
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=Narrow row
kn-keyword=Narrow row
en-keyword=Planting density
kn-keyword=Planting density
en-keyword=Podding rate
kn-keyword=Podding rate
en-keyword=Seeds/stem ratio
kn-keyword=Seeds/stem ratio
en-keyword=Seed yield
kn-keyword=Seed yield
en-keyword=Sowing time
kn-keyword=Sowing time
en-keyword=Soybean
kn-keyword=Soybean
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=17
end-page=24
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cultivar differences in nitrogen use efficiency of rice
kn-title=水稲における窒素利用効率の品種間差異
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We investigated the effects of fertilizer-free and fertilizer-applied cultivation on growth, yield and nitrogen (N) utilization of rice cultivars in our Kurashiki paddy fields (Institute of Plant Science and Resources, Okayama Univ.), which have been cultivated without fertilizer since 1970, and also in our Okayama paddy fields, which are conventionally cultivated. In 2001, the cultivars Nipponbare (NIP) and Nourin 18 (N18) were cultivated in the Kurashiki fields, with a “0N plot” (no fertilizer application), a “1N plot” (standard fertilizer application), and a “2N plot” (double fertilizer application). In 2002, five cultivars were grown without fertilizer in the Kurashiki fields, and 51cultivars were tested in 0N and 1N plots in the Okayama fields. Yield (2001) in the Kurashiki fields was higher in the 0N plot for N18 (379g m–2), which had a higher number of spikelets per m2, than NIP (300 g m–2), while in the 1N and 2N plots it was higher for NIP, which had a higher percentage of ripening, and N18 had high yield potential even without fertilizer application, but low fertilizer tolerance. The differences in yield were related to N-uptake (NU), and the differences in N use efficiency (NUE, yield/NU) between cultivars were small. The pot experiment showed that the yield of 0N plot was higher for N18 than NIP grown in Kurashiki soil because of the higher number of spikelets per hill, and the yield in the Okayama soil was higher than that in the Kurashiki soil. Long-term non-fertilized soils are of poor soil fertility, which also decreases the NUE, and the NUE of N18 is higher than that of NIP under isolated conditions. The difference in yields is closely related to sink capacity (SC). In 2002, yields in the Kurashiki fields were highest in Takanari (TAK, 494g m–2) and lowest in NIP (350g m–2), and differences in yields were closely related to SC. NUE was highest in TAK (68.6) and lowest in Akebono (48.1). TAK had high NUE and high sink production efficiency (SPE, SC/NU), while N18 had low NUE but high SC due to higher NU, ensuring high yield even under unfertilized cultivation. Yields in the 0N and 1N plots cultivated in 2002 varied between 244–631g m–2 and 199–769g m–2, respectively. A close positive correlation was observed between yield and SC, and between NU and SC, suggesting that the SC through NU is involved in determining yield. A positive correlation was also observed between NUE and yield. It was found that yield increased with an increase in NUE, and that NUE decreased although yield increased with fertilizer application. Through selection of cultivars with high SPE, it is expected that it will be possible to breed low-input, high-yielding cultivars with high NUE in the future.
en-copyright=
kn-copyright=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=1
ORCID=

en-aut-name=IwameYoshifumi
en-aut-sei=Iwame
en-aut-mei=Yoshifumi
kn-aut-name=岩目好史
kn-aut-sei=岩目
kn-aut-mei=好史
aut-affil-num=2
ORCID=

en-aut-name=MaekawaMasahiko
en-aut-sei=Maekawa
en-aut-mei=Masahiko
kn-aut-name=前川雅彦
kn-aut-sei=前川
kn-aut-mei=雅彦
aut-affil-num=3
ORCID=

en-aut-name=TakedaKazuyoshi
en-aut-sei=Takeda
en-aut-mei=Kazuyoshi
kn-aut-name=武田和義
kn-aut-sei=武田
kn-aut-mei=和義
aut-affil-num=4
ORCID=

affil-num=1
en-affil=The Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院環境生命自然科学研究科

affil-num=2
en-affil=The Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=Institute of Plant Science and Resources （IPSR）, Okayama University
kn-affil=岡山大学資源植物科学研究所

affil-num=4
en-affil=Institute of Plant Science and Resources（IPSR）, Okayama University
kn-affil=岡山大学資源植物科学研究所
en-keyword=High-yielding rice cultivar
kn-keyword=High-yielding rice cultivar
en-keyword=Nitrogen use efficiency
kn-keyword=Nitrogen use efficiency
en-keyword=Nitrogen uptake
kn-keyword=Nitrogen uptake
en-keyword=Sink capacity
kn-keyword=Sink capacity
en-keyword=Sink production efficiency
kn-keyword=Sink production efficiency
en-keyword=Unfertilized paddy field
kn-keyword=Unfertilized paddy field
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=11
end-page=16
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Genetic factors for yield related traits in lowland New Rice for Africa (NERICA)
kn-title=水稲NERICA品種の農業形質に関する遺伝的要因
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Genetic factors of yield related traits in lowland New Rice for Africa variety (NERICA) using different nitrogen condition and QTL to increase rice yield in lowland NERICAs were clarified. Indica Group rice variety (Oryza sativa L.) IR64 increased panicle number, culm weight and panicle weight, according to nitrogen concentration. The tendency is especially remarkable under low nitrogen concentration. NERICA‒L‒19, which is derived from a cross between IR64 and TOG5681 (Oryza glaberrima S.), showed similar values for panicle weight and culm weight, and it showed higher plant height, panicle weight and culm weight and lower harvest index than those of IR64 under all the concentrations. QTL analysis for the agronomic traits were performed using hybrid population derived from a cross between IR64 and NERICA‒L‒19. A total of 22 QTLs were detected on chromosomes (chrs.) 1 (3 QTLs), 2 (2), 4 (2), 5 (5), 6 (1), 7 (1), 8 (6) and 10 (2). Among them, 4 regions at 34.1‒36.6Mbp of chr. 1, at 23.8‒27.8Mbp of chr. 5 and at 4.8Mbp and 20.3‒28.4Mbp of chr. 8 showed several QTLs’ peaks overlapped. The regions of chr. 5 and 4.8Mbp of chr. 8 increased panicle weight by IR64 allele. Those of chr. 1 and 20.3-28.4Mbp of chr. 8 increased plant height and harvest index, respectively, by NERICA‒L‒19 allele. These results indicated that the larger panicle type of lowland NERICA is suitable under low nitrogen condition. Also, the plant type was controlled by the combination of 4 QTLs’ regions on chr. 1 and short arm of chr. 8 originated from O. glaberrima, and those of chr. 5 and long arm of chr. 8 from O. sativa Indica Group.
en-copyright=
kn-copyright=

en-aut-name=TomitaAsami
en-aut-sei=Tomita
en-aut-mei=Asami
kn-aut-name=冨田朝美
kn-aut-sei=冨田
kn-aut-mei=朝美
aut-affil-num=1
ORCID=

en-aut-name=AkimotoYui
en-aut-sei=Akimoto
en-aut-mei=Yui
kn-aut-name=秋元唯
kn-aut-sei=秋元
kn-aut-mei=唯
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Course of Applied Plant Science
kn-affil=応用植物科学コース

affil-num=2
en-affil=Course of Applied Plant Science
kn-affil=応用植物科学コース
en-keyword=lowland New Rice for Africa (NERICA)
kn-keyword=lowland New Rice for Africa (NERICA)
en-keyword=nitrogen
kn-keyword=nitrogen
en-keyword=agronomic traits
kn-keyword=agronomic traits
en-keyword=QTL
kn-keyword=QTL
en-keyword=rice
kn-keyword=rice
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=7
end-page=10
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of seed cold PEG-priming and subsequent long storage on germination, growth and flowering of Eustoma grandiflorum（Raf.）Shinn ʻExe Lavenderʼ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cold wet treatment is frequently applied to Eustoma seeds to enhance the bolting rate of plants grown under high temperatures. Our previous study indicated that cold PEG‒primed Eustoma seeds could maintain their germination rate and bolting rate even after being re‒dried for 30days and grown under high temperatures. The present study aimed to investigate whether prolonged storage after cold PEG‒priming affect the germination, growth, and flowering of Eustoma ‘Exe Lavender’ seedling. Seeds were initially cold‒primed with water or PEG‒6000 at ‒1.5 MPa for 5 weeks at 10°C in the dark and were then subjected to re‒drying and storage for 30, 60, 90 and 360days at 10°C. After 360days of storage, cold PEG‒primed seeds germinated earlier and more effectively than cold hydro‒primed seeds. Compared to the results after 30days of storage, plants grown from 360days exhibited similar bolting rate, days to bolting, bolting node and flowering rate, cut flower length and number of flower node. These results suggest that ‘Exe Lavender’ seeds can germinate and develop well even after 360days of storage and under high temperature conditions when subjected to cold PEG‒6000 treatment.
en-copyright=
kn-copyright=

en-aut-name=PhanThao Thu
en-aut-sei=Phan
en-aut-mei=Thao Thu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=FukushimaKeigo
en-aut-sei=Fukushima
en-aut-mei=Keigo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Hiroshima Prefectural Technology Research Institute, Agricultural Technology Research Center
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=bolting
kn-keyword=bolting
en-keyword=chilling
kn-keyword=chilling
en-keyword=dehydrate
kn-keyword=dehydrate
en-keyword=long storage
kn-keyword=long storage
en-keyword=PEG-6000
kn-keyword=PEG-6000
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Evaluator of adaptability of S. cerevisiae to grape juice using the oversxpression profiling ADOPT method
kn-title=過剰発現プロファイリングADOPT法を用いたS.cerevisiaeのワイン醸造用ブドウ果汁への適応性評価
en-subtitle=
kn-subtitle=
en-abstract=筆者らは最近，過剰発現プロファイリングADOPT 法を開発した．ADOPT 法では，出芽酵母Saccharomyces cerevisiae ゲノムのほとんどの遺伝子をそれぞれ過剰発現する酵母株を混合・競合培養し，その過程で濃縮されてきた株が過剰発現している遺伝子を体系的に同定する．さらに同定された遺伝子をたよりに，S. cerevisiae の与えられた条件での増殖に必要だが欠落しているボトルネック因子を明らかにできる．これまでの研究では，実験室で人為的に構築したストレス環境でのボトルネックの同定を行ってきたが，本研究では産業上のボトルネックを明らかにできるかをワイン醸造用のブドウ果汁を例として検証した．通常のワイン醸造に用いられる亜硫酸添加ブドウ果汁でのADOPT 実験は，亜硫酸ポンプSSU1とその転写因子FZF1の過剰発現株が強く濃縮された．SSU1機能の強化はワイン用酵母の馴養でも起きることが知られていることから，産業上のボトルネックを探索する際にもADOPT が有効であることが示された．一方，亜硫酸添加のないブドウ果汁ではADOPT で強く濃縮された遺伝子は見られず，ブドウ果汁はS. cerevisiae の増殖にとってボトルネックの少ないバランスのとれた培地であることが示唆された．
kn-abstract=The authors have recently developed the overexpression profiling ADOPT method. In the ADOPT method, yeast strains overexpressing most of the genes in the budding yeast Saccharomyces cerevisiae genome are mixed and competitively cultured, and the genes overexpressed in the enriched strains are systematically identified. Furthermore, the identified genes can be used to identify bottleneck factors that are necessary but lacking for growth of S. cerevisiae under given conditions. In our previous studies, we have identified bottlenecks in artificially created stress environments in the laboratory, but in this study, we used grape juice for winemaking as an example to see if industrial bottlenecks can be identified. ADOPT experiments with sulfite-added grape juice used in conventional winemaking resulted in a strong enrichment of strains overexpressing the sulfite pump SSU1 and its transcription factor FZF1. Since enhancement of SSU1 function is known to occur in wine yeast acclimation, ADOPT was also shown to be useful in the search for industrial bottlenecks. On the other hand, no genes were strongly enriched by ADOPT in grape juice without sulfite addition, suggesting that grape juice is a balanced medium with few bottlenecks for S. cerevisiae growth.
en-copyright=
kn-copyright=

en-aut-name=MoriyaHisao
en-aut-sei=Moriya
en-aut-mei=Hisao
kn-aut-name=守屋央朗
kn-aut-sei=守屋
kn-aut-mei=央朗
aut-affil-num=1
ORCID=

en-aut-name=OnoChiyuki Kohata 
en-aut-sei=Ono
en-aut-mei=Chiyuki Kohata 
kn-aut-name=小野千由貴
kn-aut-sei=小野
kn-aut-mei=千由貴
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Course of Agrochemical Bioscience
kn-affil=農芸化学コース

affil-num=2
en-affil=Course of Agrochemical Bioscience
kn-affil=農芸化学コース
en-keyword=yeast
kn-keyword=yeast
en-keyword=S. cerevisiae
kn-keyword=S. cerevisiae
en-keyword=overexpression
kn-keyword=overexpression
en-keyword=wine making
kn-keyword=wine making
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=113
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=23
end-page=36
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2022
kn-title=公表学術論文等リスト　2022
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=19
end-page=22
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Sexual Dimorphism of Arginine Vasotocin Neuron in Birds
kn-title=鳥類におけるアルギニンバソトシン神経の性差
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Arginine vasotocin (AVT) is one of neurohypophysial peptides in birds and is well known both as an antidiuretic and oxytocic hormone. AVT is produced in the hypothalamus and mainly synthesized in magnocellular neurons, supraoptic nucleus (SON) and paraventricular nucleus (PVN). Recent, immunocytochemistry and in situ hybridization results indicate that AVT is also synthesized in parvocellular neurons corresponding to mammalian stria terminalis (nST). The AVT neurons in nST in the male are more numerous than those in the female. Therefore, these data suggest that there is sexual dimorphism in the distribution of AVT neurons. Several experiments involving electrical lesion, testosterone implantation, and the detection of the immediately-early gene expression in birds expressing copulatory behavior suggested that nST and nucleus praeopticus medialis (POM) were related to the reproductive behavior. When male embryo was treated with estradiol, the distribution of AVT neurons assumes the same distribution as found in the female, and reproductive behavior was abolished, suggesting that AVT in the brain has an important role in reproductive behavior.
en-copyright=
kn-copyright=

en-aut-name=SaitoNoboru
en-aut-sei=Saito
en-aut-mei=Noboru
kn-aut-name=齋藤昇
kn-aut-sei=齋藤
kn-aut-mei=昇
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Course of Applied Animal Science
kn-affil=応用動物科学コース
en-keyword=arginine vasotocin
kn-keyword=arginine vasotocin
en-keyword=sexual dimorphism
kn-keyword=sexual dimorphism
en-keyword=reproductive behavior
kn-keyword=reproductive behavior
en-keyword=parvocellular neurons
kn-keyword=parvocellular neurons
en-keyword=bird
kn-keyword=bird
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=13
end-page=18
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on l-Glutamate Oxidase with Strict Substrate Specificity from Streptomyces sp.
kn-title=Streptomyces属放線菌由来の高基質特異性l-グルタミン酸オキシダーゼに関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=l-glutamate oxidase (LGOX) from Streptomyces sp. is a heterohexameric flavin enzyme that catalyzes the oxidative deamination of l-glutamate to form α-ketoglutarate with ammonia and hydrogen peroxide. LGOX shows strict substrate specificity for l-Glu. In addition, it is highly thermostable and pH stable. Because of these properties, LGOX is currently used as a biosensor for the trace determination of l-Glu in the food industry and clinical laboratories. The full-length cDNA is 2103 bp and is encoded by a single polypeptide chain consisting of 701 residues including subunits α-γ-β. The LGOX gene was heterologously expressed in Escherichia coli JM109. The LGOX precursor expressed in E. coli is a homodimer with weak enzymatic activity and becomes a heterohexamer upon activation by protease treatment. X-ray crystallography and docking studies of purified recombinant LGOX suggest that the Arg305 residue is a key residue for substrate recognition. Mutant analysis showed that Arg305 is essential for substrate recognition, as the activity toward l-Glu was greatly reduced and substrate specificity was changed in some enzymes. The functional analysis of R305E-LGOX, which is an l-Arg oxidase, revealed that R305E-LGOX can be used as a enzyme biosensor for l-Arg.
en-copyright=
kn-copyright=

en-aut-name=NakayamaNatsume
en-aut-sei=Nakayama
en-aut-mei=Natsume
kn-aut-name=中山夏女
kn-aut-sei=中山
kn-aut-mei=夏女
aut-affil-num=1
ORCID=

en-aut-name=InagakiK.
en-aut-sei=Inagaki
en-aut-mei=K.
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Course of Agrochemical Bioscience
kn-affil=農芸化学コース

affil-num=2
en-affil=
kn-affil=岡山大学学術研究院環境生命科学学域
en-keyword=l-glutamate oxidase
kn-keyword=l-glutamate oxidase
en-keyword=biosensor
kn-keyword=biosensor
en-keyword=substrate recognition
kn-keyword=substrate recognition
en-keyword=X-ray crystallography
kn-keyword=X-ray crystallography
en-keyword=modification of substrate specificity
kn-keyword=modification of substrate specificity
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=7
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of seed cold PEG–priming concentration and period followed by re–drying on germination, growth and flowering of Eustoma grandiflorum （Raf.） Shinn ʻExe Lavenderʼ
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study aimed to investigate whether seed cold priming by PEG‒6000, followed by re‒drying would affect the germination, growth, and flowering of Eustoma ‘Exe Lavender’ raised seedling under high temperatures. Seeds of control treatment were not treated with cold‒priming or re‒drying. In experiment 1, seeds were cold‒primed with water and PEG‒6000 at ‒0.75 and ‒1.5 MPa for 5 weeks at 10°C in the dark and then subjected to re‒dried and storage for 30 days at 10°C. The results showed that cold‒primed seeds germinated earlier than those of the control. Compared to water treatment, plants grown from PEG‒6000 treatments and re‒drying showed similar germination rate, bolting rate, days to bolting, bolting node and flowering rate. In experiment 2, cold priming periods by water and ‒1.5 MPa PEG‒6000 were investigated for 4, 5, 6, 7, and 8 weeks. ‘Exe Lavender’ seeds treated with cold water and in the dark for longer than 5 weeks increased their percentage of radicle emergence and decreased germination rate after 30 days of re‒drying and storage. Meanwhile, those treated with cold-PEG did not protrude the radicle even for 8 weeks of priming and maintained the germination rate after re‒drying and storage. Days to bolting and days to flowering tended to decrease when the priming period decreased. The results suggest that the seed cold priming treatment by ‒1.5 MPa PEG‒6000 and subsequent re‒drying improved germination rate and maintained bolting rate and flowering rate in Eustoma compared to cold priming treatment by water raised seedling under high temperature conditions.
en-copyright=
kn-copyright=

en-aut-name=Thao Thu Phan
en-aut-sei=Thao Thu Phan
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=FukushimaKeigo
en-aut-sei=Fukushima
en-aut-mei=Keigo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Hiroshima Prefectural Technology Research Institute, Agricultural Technology Research Center
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=bolting
kn-keyword=bolting
en-keyword=chilling
kn-keyword=chilling
en-keyword=dehydrate
kn-keyword=dehydrate
en-keyword=PEG-6000
kn-keyword=PEG-6000
en-keyword=storage
kn-keyword=storage
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Comparison of LEDs, Fluorescent Lamps and Incandescent Bulbs for Long-day Treatment of Forcing Strawberries as Affected by Respective Color Temperature
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Long day (LD) treatment is frequently applied for horticultural crops, including strawberry, and sufficient effect similar to 4 to 6hours of day time elongation in the evening can be achieved by 1 to 3hours of night break in the mid night. Incandescent lamps (IC) have long been an important light source, but the general incandescent lamps are out of production and have been replaced with LED or florescent lamps (FL). Thus, we compared light sources and their color temperature (2,800K, warm white similar to IC; 6,500K, daylight) in different lighting programs. The effectiveness of 3 and 4cycles of alternate intermittent lighting, 30min on/30min off, were equal to and larger than 2hours of night break, respectively. Measured intensity of illumination (lux) at the canopy surface by 2,800K LED (6.9W) and FL (12W) was 1.50 and 0.95times as large as IC (54W), but leaf growth under LED and FL was similar to and less vigorous than IC, respectively. On the other hand, intensity values of illumination by 6,500K LED (6.9W) and FL (12W) were both 1.20times as large as 2,800K ones, but 6,500K light sources were much less effective compared to 2,800K ones. Daylight type LED and FL which have higher luminance efficiency but emit less red light compared to warm white ones, are not suitable as alternative light sources for IC.
en-copyright=
kn-copyright=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HanadaAtsushi
en-aut-sei=Hanada
en-aut-mei=Atsushi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OogumaKeita
en-aut-sei=Ooguma
en-aut-mei=Keita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YoshidaKenta
en-aut-sei=Yoshida
en-aut-mei=Kenta
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Faculty of Agriculture, Okayama University
kn-affil=

affil-num=4
en-affil=Japan Agency for Marine-Earth Science and Technology
kn-affil=

affil-num=5
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=

affil-num=7
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=daylight
kn-keyword=daylight
en-keyword=Fragaria ×ananassa Duch.
kn-keyword=Fragaria ×ananassa Duch.
en-keyword=intermittent lighting
kn-keyword=intermittent lighting
en-keyword=luminance efficiency
kn-keyword=luminance efficiency
en-keyword=warm white
kn-keyword=warm white
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=112
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=111 Supplement
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Proceedings of the International Symposium on Animal Bioscience 2021
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=1
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=27
end-page=42
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2021
kn-title=公表学術論文等リスト　2021
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=21
end-page=25
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Propagation characteristics and method of appropriate management of invasive alien species Iris pseudacorus L.
kn-title=外来水生植物キショウブの繁殖特性と適切な管理法
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=To establish a proposal for a guiding principle supporting appropriate management of Iris pseudacorus L., which had been used as an important vegetation bank species until the Invasive Alien Species Act, this study investigated propaga-tion characteristics. The seed in which seed coat cracked germinated earlier, and it was shown that their cumulative germination percentage was high even under 30 °C constant conditions. When the fresh weight of the rhizome cut pieces in the spring became greater than 15 g, the individual plant flowering rate increased gradually. Individuals of more than 30 g achieved a flowering rate higher than 76 %. The flow-ering rate was highest for individual plants that had two green leaves, but it became progressively lower for increas-ing numbers of green leaves：non-flowering individuals grew better than flowering individuals. In the 0 cm height scape-cutting treatment, no significant difference was found in the numbers of flowers and fruits the next year. In the 5 cm height top-cutting treatment, the flowers and fruits in the next year were significantly fewer. 
en-copyright=
kn-copyright=

en-aut-name=NakashimaYoshitaka
en-aut-sei=Nakashima
en-aut-mei=Yoshitaka
kn-aut-name=中嶋佳貴
kn-aut-sei=中嶋
kn-aut-mei=佳貴
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Course of Applied Plant Science, The Faculty of Agriculture, Okayama University
kn-affil=岡山大学農学部　応用植物科学コース
en-keyword=Iris pseudacorus L.
kn-keyword=Iris pseudacorus L.
en-keyword=invasive alien species
kn-keyword=invasive alien species
en-keyword=propagation characteristics
kn-keyword=propagation characteristics
en-keyword=flood tolerance
kn-keyword=flood tolerance
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=15
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Big data in the sex chromosomes of Silene plants and a fungus acting as a plant sex chromosome
kn-title=Silene 属植物が保持する性染色体ビッグデータと性操作する菌
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Silene latifolia (Caryophyllaceae) is a dioecious plant that has long been used for study on sex chromosomes in plants. The advantage but also disadvantage of S. latifolia as a model system is the size of the Y chromosome that contains an extremely large male-specific region (approx. > 500 Mb). This feature implies that the evolutionary history of sex chromo-somes remains in the S. latifolia Y chromosome, while the size makes analyses esoteric. Another advantage is that plants in the genus Silene show variation in reproductive systems; most are gynodioecy (females and hermaphrodites), which is thought of as an evolutionary status before establishment of dioecy (males and females), with a few hermaphrodites and dioecy, suggesting that the genus Silene may represent an epitome of the sex chromosome evolution. Microbotryum is a biotrophic fungi, whose infection causes masculinization of the female flower, as if the fungus acts as the Y chromo-some. Though the underlying molecular mechanisms remain unknown, recent high-throughput sequence technologies provide many candidate genes for sex determination in plants and sex conversion by the fungus. In this article, I review and introduce studies of the Y chromosome in  S. latifolia plant, the evolution of sex chromosomes in the genus Silene, the masculinization of female flowers caused by a fungus infection, and a virus vector that can be used for genetic analysis of the key genes involved in these processes.
en-copyright=
kn-copyright=

en-aut-name=FujitaNaoko
en-aut-sei=Fujita
en-aut-mei=Naoko
kn-aut-name=藤田尚子
kn-aut-sei=藤田
kn-aut-mei=尚子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Course of Applied Plant Science, The Faculty of Agriculture, Okayama University
kn-affil=岡山大学農学部　応用植物科学コース
en-keyword=Silene latifolia
kn-keyword=Silene latifolia
en-keyword=Microbotryum lychnidis-dioicae
kn-keyword=Microbotryum lychnidis-dioicae
en-keyword=anther smut
kn-keyword=anther smut
en-keyword=sex chromosome
kn-keyword=sex chromosome
en-keyword=plant-microbe interaction
kn-keyword=plant-microbe interaction
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=7
end-page=14
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on antitumor enzyme l-lysine α-oxidase from Trichoderma viride
kn-title=糸状菌Trichoderma viride由来の抗腫瘍性酵素L-リシン α-オキシダーゼに関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=L-Lysine α-oxidase (LysOX) from Trichoderma viride is a homodimeric flavoenzyme that catalyzes the oxidative deamination of L-Lysine to produce α-keto-ε-aminocaproate with ammonia and hydrogen per-oxide. LysOX inhibited the growth of cancer cells but showed relatively low toxicity for normal cells. The full-length cDNA consists of 2,119 bp, and encodes a long N-terminal propeptide composed of 77 resi-dues (Met1-Arg77) and the mature protein (Ala78-Ile617). The LysOX gene was heterologously expressed in Streptomyces lividans TK24 or Escherichia coli SoluBL21. The enzymatic properties of the purified recombinant LysOX, such as substrate specificity, kinetic parameters and thermal stability, are the same as those of the native LysOX. The LysOX precursor (prLysOX) expressed in E. coli shows weak enzymatic activity and is activated by proteolytic processing. The crystal structure of prLysOX revealed that the propeptide of prLysOX indirectly changes the active site structure to inhibit enzyme activity. Moreover, the crystal structures of LysOX and its L-Lysine complex revealed that the hydrogen bonding network formed by Asp212, Asp315 and Ala440 with two water molecules is responsible for the recogni-tion of the ε-amino group of L-Lysine. In addition, a narrow substrate-binding site and acidic surface at the active site entrance both contribute to strict substrate specificity. Mutational analysis demonstrated that Asp212 and Asp315 are essential for substrate recognition, and the D212A/D315A LysOX prefers aromatic amino acids. Furthermore, the structural basis of the substrate specificity change has also been revealed by the structural analysis of the D212A/D315A LysOX and its substrate complexes. 
en-copyright=
kn-copyright=

en-aut-name=SaitoMasaya
en-aut-sei=Saito
en-aut-mei=Masaya
kn-aut-name=齋藤雅哉
kn-aut-sei=齋藤
kn-aut-mei=雅哉
aut-affil-num=1
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Course of Agrochemical Bioscience
kn-affil=岡山大学農学部農芸化学コース

affil-num=2
en-affil=
kn-affil=学術研究院環境生命科学学域
en-keyword=L-lysine α-oxidase
kn-keyword=L-lysine α-oxidase
en-keyword=antitumor enzyme
kn-keyword=antitumor enzyme
en-keyword=substrate recognition
kn-keyword=substrate recognition
en-keyword=X-ray crystallography
kn-keyword=X-ray crystallography
en-keyword=enzyme activity regulation
kn-keyword=enzyme activity regulation
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of Re-Drying and Storage after 10°C Wet Treatment on Germination and Growth of Five Eustoma grandiflorum (Raf.) Shinn Cultivars
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In Eustoma (Eustoma grandiflorum (Raf.) Shinn) cultivation in southwestern Japan, cold imbibition treatment of seeds is important to accelerate bolting and flowering of seedlings grown in the summer season. In order to facilitate the handling of cold imbibed seeds, re-drying treatment (RDT) after wet treatment at 10°C for 35 days in dark conditions (WT10°C) is considered to be an essential technique. The investigation of five Eustoma cultivars ‘Exe Lavender’, ‘Orb Snow’, ‘Philia Lavender’ , ‘Dure Lavender’ and ‘Exe Light Pink’ was to find the germinability and the growth characteristics of Eustoma when re-drying and storage at 10°C for 30 days were applied immedietely after ending the WT10°C. The results showed that germination and growth responses differed among Eustoma cultivars. RDT seeds and non- RDT seeds of ‘Exe Lavender’, ‘Orb Snow’ and ‘Philia Lavender’ had a similar germination rate. However, germination rate of RDT seeds in ‘Dure Lavender’ and ‘Exe Light Pink’ was lower than that of non-RDT seeds. There was no significant difference in cut-flower quality grown from RDT and non-RDT among ‘Exe Lavender’, ‘Orb Snow’ and ‘Dure Lavender’. However, bolting and flowering rate of RDT in ‘Dure Lavender’ and ‘Exe Light Pink’ were lower than those of non-RDT. This suggested that it might not be useful to apply RDT after the cold-wet treatment of Eustoma seed due to lower germination or bolting rates in some cultivars. Further investigation of cold-wet exposure and dehydrating conditions is required to establish RDT procedures for Eustoma cultivars.
en-copyright=
kn-copyright=

en-aut-name=Thao Thu Phan
en-aut-sei=Thao Thu Phan
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=FukushimaKeigo 
en-aut-sei=Fukushima
en-aut-mei=Keigo 
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science
kn-affil=

affil-num=2
en-affil=Hiroshima Prefectural Technology Research Institute, Agricultural Technology Research Center
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and Life Science
kn-affil=

affil-num=5
en-affil=Graduate School of Environmental and Life Science
kn-affil=

affil-num=6
en-affil=Graduate School of Environmental and Life Science
kn-affil=
en-keyword=bolting
kn-keyword=bolting
en-keyword=chilling
kn-keyword=chilling
en-keyword=cut flower quality
kn-keyword=cut flower quality
en-keyword=dehydrate
kn-keyword=dehydrate
en-keyword=flowering
kn-keyword=flowering
END

start-ver=1.4
cd-journal=joma
no-vol=111
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=15
end-page=31
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2020.
kn-title=公表学術論文等リスト　2020
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=7
end-page=13
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies for stabilization of flower color and cut flower longevity
kn-title=花色の安定化と切り花の延命に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　My research interest is in the physiology and ecology of ornamental plants. Here, I introduce my research subjects on the stabilization of flower color and cut flower longevity. Seasonal color change of dahlia (Dahlia variabilis) is studied as practical study for improving the stable dahlia cut flower production during autumn and winter. The results obtained from pigment analysis and clone selection are presented. Research on cut flower longevity is presented from two aspects. Firstly, study on the structure of conductive tissues that contribute to water transport is presented. Secondly, study on the floral organs that contribute to transpiration from cut flowers is presented.
en-copyright=
kn-copyright=

en-aut-name=KitamuraYoshikuni
en-aut-sei=Kitamura
en-aut-mei=Yoshikuni
kn-aut-name=北村嘉邦
kn-aut-sei=北村
kn-aut-mei=嘉邦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=cut flower
kn-keyword=cut flower
en-keyword=flower color
kn-keyword=flower color
en-keyword=transpiration
kn-keyword=transpiration
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A comparative gene analysis reveals a diatom-specific SET domain protein family
kn-title=遺伝子比較解析による珪藻類特異的なSET ドメインタンパク質ファミリーの同定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　The silica cell walls of diatoms, which exhibit species-spe-cific micro- and nano- patterned structures are promising candidates for applications in nanotechnology. Previous studies revealed a number of silica cell wall-associated proteins involved in silica formation. However, molecular biological analyses toward understanding of diatom cell wall formation have been mostly limited to model diatom species and general silica formation process in diatoms is still incompletely understood. In this study, to gain a compre-hensive insight into diatom silica biomineralization, tran-scriptome data of three diatom species, Nitzschia palea, Achnanthes kuwaitensis and Pseudoleyanella lunata, were newly developed. The reads obtained from RNA sequencing were assembled into 31,946, 60,767 and 38,314 unique transcripts for N. palea, A. kuwaitensis and P. lunata, respectively. In order to identify the diatom-specific genes, three transcriptome data sets developed in this study and the protein-coding gene sets of five genome-sequenced diatoms were compared. The proteins shared only by eight diatom species that are predicted to possess an endoplasmic reticulum (ER)-targeting signal peptide were selected for further analyses. These include proteins showing homology to silicanin-1, a recently reported diatom-specific protein involved in silica formation, as well as a number of SET domain proteins. The SET domain proteins might be novel diatom-specific family of methyltransferases that may reg-ulate the function of silica formation related proteins or long chain polyamines. The genes encoding the diatom-specific SET domain proteins identified in this study, which were shown to respond to silicon were suggested to be implicated in silica biomineralization.  
en-copyright=
kn-copyright=

en-aut-name=NemotoMichiko
en-aut-sei=Nemoto
en-aut-mei=Michiko
kn-aut-name=根本理子
kn-aut-sei=根本
kn-aut-mei=理子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=Biomineralization
kn-keyword=Biomineralization
en-keyword=Diatom
kn-keyword=Diatom
en-keyword=Silica
kn-keyword=Silica
en-keyword=Protein
kn-keyword=Protein
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=110
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=49
end-page=4961
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2019.
kn-title=公表学術論文等リスト2019
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=41
end-page=47
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Genetic mechanism for soil-surface roots originating from a New Plant Type rice (Oryza sativa L.)
kn-title=イネ地表根に関する遺伝的機構の解明
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= A new method of using seedling trays to evaluate root angle distribution in rice (Oryza sativa. L) was developed. By using this method, the root angle distributions of 97 accessions were characterized into two cluster groups ; A and B. The numbers of accessions in group A were limited, and these were categorized as shallow rooting types including soil-surface root. Group B included from shallow to deep rooting types, including both Indica and Japonica Group cultivars, lowland and upland cultivars, and landraces and improved types. An introgression line YTH16 harboring chromosome segments from a New Plant Type cultivar IR65600-87-2-2-3 with genetic background of an Indica Group rice IR 64, was included in Group A. To clarify the genetic mechanism for soil-surface rooting, quantitative trait loci (QTL) analysis was performed using hybrid populations derived from a cross between IR 64 and YTH16. A total of 8 QTLs were detected in the 3 introgressed segments on chromosomes (chr.) 2, 5 and 7. Seven chromosome segment lines (CSLs) combining these 3 QTL regions were selected from the progenies. The 2 CSLs harboring a single region (excluding the CSL with a region on chr. 5) showed high soil-surface root scores and low root vertical angles (RVA) in comparison with IR 64. Four CSLs harboring 2 or 3 regions showed high scores and low RVAs
in comparison with YTH16 and the CSLs harboring a single QTL region. These results indicated that the soil-surface and shallow rooting of YTH16 was controlled by the 2 major QTLs’ regions on chrs. 2 and 7, and that chr. 5 particularly played a role for supporting the effect with them.
en-copyright=
kn-copyright=

en-aut-name=TomitaAsami
en-aut-sei=Tomita
en-aut-mei=Asami
kn-aut-name=冨田朝美
kn-aut-sei=冨田
kn-aut-mei=朝美
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院 環境生命科学研究科
en-keyword=root angle distribution
kn-keyword=root angle distribution
en-keyword=soil-surface root
kn-keyword=soil-surface root
en-keyword=New Plant Type
kn-keyword=New Plant Type
en-keyword=QTL
kn-keyword=QTL
en-keyword=rice (Oryza sativa L.)
kn-keyword=rice (Oryza sativa L.)
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=37
end-page=40
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Sexuality in plants, unveiled from genome evolution
kn-title=ゲノム進化が紐解く植物の性決定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Sexual polymorphism, a main strategy to maintain genetic diversity within a species, has long been a major focus in biology. Notwithstanding, in plants, evolution of sexual systems and mechanisms underlying these transitions have been little unveiled. We have elucidated the molecular mechanism of sex determination in persimmons (Diospyros spp.), where the Y-encoded smRNA gene OGI can repress the female-determining gene MeGI, and also in kiwifruits (Actinidia spp.), where the Y-encoded two sex determinants, Shy Girl and Friendly Boy, control gynoecium and androecium development, respectively. Although the molecular functions of these determinants are distinct, they have common evolutionary scenarios involving transitions of sexual systems. In persimmon, a recent genome triplication (hexaploidization) in cultivated persimmon (D. kaki) derived “flexible” sexuality via establishing epigenetic layers on the two sex determinants. On the other hand, an ancient Diospyros-specific paleo-genome duplication (paleo-tetraploidization) enabled neofunctionalization in the proto-MeGI, via positive selection, to establish a new function as a sex determinant. In kiwifruit, one of the two sex determinants, Shy Girl, was derived from neofunctionalization via Actinidia-specific duplication event. These findings exemplify how plant-specific numerous duplication events can drive flexible genetic material whose variation can be selected for development of new sexual systems.
en-copyright=
kn-copyright=

en-aut-name=AkagiTakashi
en-aut-sei=Akagi
en-aut-mei=Takashi
kn-aut-name=赤木剛士
kn-aut-sei=赤木
kn-aut-mei=剛士
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
en-keyword=Sex determination
kn-keyword=Sex determination
en-keyword=Sex chromosome
kn-keyword=Sex chromosome
en-keyword=Polyploidization
kn-keyword=Polyploidization
en-keyword=Genome evolution
kn-keyword=Genome evolution
en-keyword=Tree crops
kn-keyword=Tree crops
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=29
end-page=36
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Development of Effective Biological Treatment Process for Acid Mine Drainage
kn-title=酸性鉱山廃水の 効果的な生物的処理プロセスの開発
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Acid mine drainage (AMD) is acidic and generally enriched with iron, aluminum, sulfate and heavy metals, such as lead and cadmium. AMD is a growing problem of emerging concern that cause detrimental effects to the environment and living organisms. Yanahara mine in Misaki Town, Okayama, Japan, had mainly produced pyrite for sulfuric acid manufacture. Although it was closed in 1991, AMD is being generated from the mine now. A passive treatment based on the biological oxidation of ferrous iron is a promising strategy for AMD remediation. AMD from Yanahara mine is treated in a plant using iron-oxidizing bacteria, Acidithiobacillus ferrooxidans and Ferrovum spp. The AMD generation continues for several centuries with dramatic consequences on the receiving environments. Therefore, the development of sustainable and cost effective treatment process is required. A development of the effective biological treatment process with an iron oxidation reactor operated at pH 3.5 is described in this report. Economic aspects are also discussed.
en-copyright=
kn-copyright=

en-aut-name=KamimuraKazuo
en-aut-sei=Kamimura
en-aut-mei=Kazuo
kn-aut-name=上村一雄
kn-aut-sei=上村
kn-aut-mei=一雄
aut-affil-num=1
ORCID=

en-aut-name=KanaoTadayoshi
en-aut-sei=Kanao
en-aut-mei=Tadayoshi
kn-aut-name=金尾忠芳
kn-aut-sei=金尾
kn-aut-mei=忠芳
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=acid mine drainage
kn-keyword=acid mine drainage
en-keyword=bioremediation
kn-keyword=bioremediation
en-keyword=iron-oxidizing bacteria
kn-keyword=iron-oxidizing bacteria
en-keyword=microbial community
kn-keyword=microbial community
en-keyword=Acidithiobacillus ferrooxidans
kn-keyword=Acidithiobacillus ferrooxidans
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=21
end-page=27
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Defoliating to 12-15 leaves increases calcium concentration and decreases blossom-end rot incidence in fruit of tomato plant grown under moderate water stress
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= The objectives of this study were to (i) determine the optimum number of whole leaves to retain on a tomato plant for effective blossom-end rot (BER) management and (ii) explore the relationship between shoot calcium (Ca) and fruit Ca in non-defoliated plants in two different sized fruit cultivars, a large-fruited cultivar ‘Momotaro fight’ and a medium-fruited cultivar ‘Cindy sweet’. Treatments involved maintaining 18, 15 and 12 leaves on a plant. All lateral shoots were removed regularly throughout the growing period except the shoot closest to the flowering truss in the 18-leaf treatment. At the length of 10cm, these shoots were sampled for real time Ca determination using a hand held Ca2+ meter. In the plants defoliated to 18 leaves, BER was higher in ‘Momotaro fight’ at 10% compared to 2% in ‘Cindy sweet’. Fruit growth rate was significantly increased by defoliation in ‘Momotaro fight’, however no significant difference was observed among treatments in ‘Cindy sweet’. Defoliating to 12 leaves increased daily Ca transport rate by 59% and 37% in ‘Momotaro fight’ and ‘Cindy sweet’, respectively. Defoliating to 12 leaves increased the water-soluble Ca concentration in the distal part of fruit by 34% and 14% in ‘Momotaro fight’ and ‘Cindy sweet’, respectively. In the plants defoliated to 18 leaves where only old yellowish leaves were removed, a significant steady decrease was observed in the concentration of water soluble Ca in the distal part of fruit with increase in truss order. There was a significant linear relationship between water-soluble Ca concentration in the distal part of the fruit and Ca concentration in the lateral shoot of plants defoliated to 18 leaves. We conclude that under moderate water stress by root zone restriction and also certain other BER inductive conditions, defoliation to 12–15 leaves on a tomato plant should be a promising approach for decreasing BER incidence in susceptible large fruit cultivars.
en-copyright=
kn-copyright=

en-aut-name=Annah Khatenje Indeche
en-aut-sei=Annah Khatenje Indeche
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MiyajiDaisuke
en-aut-sei=Miyaji
en-aut-mei=Daisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate school of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Graduate school of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Field Science Center
kn-affil=

affil-num=4
en-affil=Graduate school of Environmental and Life Science, Okayama University
kn-affil=

affil-num=5
en-affil=Graduate school of Environmental and Life Science, Okayama University
kn-affil=

affil-num=6
en-affil=Graduate School of Agriculture, Kyoto University
kn-affil=
en-keyword=BER management
kn-keyword=BER management
en-keyword=defoliation
kn-keyword=defoliation
en-keyword=water-soluble Ca
kn-keyword=water-soluble Ca
en-keyword= lateral shoot Ca
kn-keyword= lateral shoot Ca
en-keyword=root zone restriction
kn-keyword=root zone restriction
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=13
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effect of System of Rice Intensification（SRI）on growth and yield performance in Nam Dong district, Thua Thien Hue province, Vietnam
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= The field study was conducted in Nam Dong district, Thua Thien Hue province, Vietnam during May 2019 to observe the effects of SRI method on the rice yield and eradicate the factor affecting the yield. The study was carried out to evaluate the agronomic characteristics of rice, fresh and dry matter production, soil characteristics with a focus group discussion regarding cultivation by SRI and non-SRI methods. The soil analyses using standard measurements showed quite similar soil fertility in both SRI and non-SRI methods, while rice plants showed good agronomic performances and fresh and dry matter production in SRI method. At harvesting time, the yield and yield potentials of rice were greater in SRI than that in non-SRI methods. Focus group discussion found some factors that limited SRI development in Nam Dong district.
en-copyright=
kn-copyright=

en-aut-name=Nguyen Quang Co
en-aut-sei=Nguyen Quang Co
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Nguyen Thi Thanh Hien
en-aut-sei=Nguyen Thi Thanh Hien
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=University of Agriculture and Forestry, Hue Univer
kn-affil=

affil-num=2
en-affil=Institute of Resources and Environment, Hue University
kn-affil=

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=Actual yield
kn-keyword=Actual yield
en-keyword=Focus group discussion
kn-keyword=Focus group discussion
en-keyword=Filled spikelets
kn-keyword=Filled spikelets
en-keyword=SRI
kn-keyword=SRI
en-keyword=Weeding
kn-keyword=Weeding
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=7
end-page=11
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells
kn-title=酵母2μプラスミドのコピー数変動の数理的解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= 　Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency （fitness） produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy.
en-copyright=
kn-copyright=

en-aut-name=SaekiNozomu
en-aut-sei=Saeki
en-aut-mei=Nozomu
kn-aut-name=佐伯望
kn-aut-sei=佐伯
kn-aut-mei=望
aut-affil-num=1
ORCID=

en-aut-name=MoriyaHisao
en-aut-sei=Moriya
en-aut-mei=Hisao
kn-aut-name=守屋央朗
kn-aut-sei=守屋
kn-aut-mei=央朗
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University)
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=2
en-affil= Research Core for Interdisciplinary Sciences, Okayama University
kn-affil=異分野融合先端研究コア
en-keyword=yeast
kn-keyword=yeast
en-keyword=2 μ plasmid
kn-keyword=2 μ plasmid
en-keyword=mathematical model
kn-keyword=mathematical model
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea
kn-title=Marinomonas mediterranea由来キノン含有新規グリシンオキシダーゼの大腸菌発現系の確立と性質検討
en-subtitle=
kn-subtitle=
en-abstract=, , , 
kn-abstract= 　Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity.
en-copyright=
kn-copyright=

en-aut-name=KajiyamaYuki
en-aut-sei=Kajiyama
en-aut-mei=Yuki
kn-aut-name=梶山雄輝
kn-aut-sei=梶山
kn-aut-mei=雄輝
aut-affil-num=1
ORCID=

en-aut-name=MizobataSatsuki
en-aut-sei=Mizobata
en-aut-mei=Satsuki
kn-aut-name=溝端佐津紀
kn-aut-sei=溝端
kn-aut-mei=佐津紀
aut-affil-num=2
ORCID=

en-aut-name=AkajiShusaku
en-aut-sei=Akaji
en-aut-mei=Shusaku
kn-aut-name=赤地周作
kn-aut-sei=赤地
kn-aut-mei=周作
aut-affil-num=3
ORCID=

en-aut-name=NemotoMichiko
en-aut-sei=Nemoto
en-aut-mei=Michiko
kn-aut-name=根本理子
kn-aut-sei=根本
kn-aut-mei=理子
aut-affil-num=4
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=5
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=6
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=3
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=5
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=glycine oxidase
kn-keyword=glycine oxidase
en-keyword=Marinomonas mediterranea
kn-keyword=Marinomonas mediterranea
en-keyword=cysteine tryptophilquinone
kn-keyword=cysteine tryptophilquinone
en-keyword=recombinant expression
kn-keyword=recombinant expression
en-keyword=enzymatic glycine assay
kn-keyword=enzymatic glycine assay
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=109
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次 
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=19
end-page=32
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2018
kn-title=公表学術論文等リスト 2018
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=15
end-page=18
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Structural Features of Pollen Allergens N-Glycans and Synthesis of Glycopolymer carrying Multivalent N-glycans
kn-title=花粉アレルゲンの糖鎖構造特性と糖鎖ポリマーの合成
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=MaedaMegumi
en-aut-sei=Maeda
en-aut-mei=Megumi
kn-aut-name=前田恵
kn-aut-sei=前田
kn-aut-mei=恵
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院　環境生命科学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=5
end-page=13
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of boron deficiency on tip burn and malformed fruit incidence in strawberries
kn-title=B（ホウ素）欠乏処理がイチゴのチップバーンおよび受精不良果発生に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=　B（ホウ素）は植物にとって必須な微量要素であり，イチゴの受精不良果発生要因の1 つである．そこで2015年度と2016年度の2 回にわたりB 欠乏がイチゴの受精不良果発生に及ぼす影響について調査した．また，2016年度はB 欠乏処理後にB 回復処理を行い，その後のイチゴの果実形態の変化についても調査した．その結果，B欠乏処理を行うと蒸散機能の低い新葉や花芽においてチップバーンが発生し，種浮き果や部分不受精を主とした受精不良果が多発した．しかし，B 回復処理を行うことでこれらの症状が改善することが明らかになった．B は受精不良果発生に関係しており，欠乏条件下で根から吸収させると急速に若い成長中の組織に転流することが示されたことから，B 栄養をコントロールすることでB 不足によるイチゴの受精不良果発生を軽減できる可能性があると考えられる．
kn-abstract=Boron (B) is an essential micro element for plants and plays important roles in the synthesis and functions of cell wall. B deficiency has been reported as one of the causes of fruit malformation in strawberries. We investigated the effect of B deficiency on flower and fruit development of forced strawberries for two cropping seasons (2015-2017). In the second season, B was resupplied for B-deficient plants and we investigated changes in fruit development. When B-free nutrient solutions were supplied, tip burn began to occur in newly emerging leaves and calyx 2 to 3 months later, and fruit malformation including seedy or only partly developed fruits with undeveloped achenes occurred frequently. However, these deficient symptoms were quickly disappeared by supplying B containing nutrient solutions. In conclusion, B nutrition is closely related to the occurrence of fruit malformation through fertility of pollen and pistils, and also development of receptacle tissue in strawberries. It should be possible to reduce fruit malformation in strawberries by proper control of B nutrition.
en-copyright=
kn-copyright=

en-aut-name=SesumiMiho
en-aut-sei=Sesumi
en-aut-mei=Miho
kn-aut-name=瀬角美穂
kn-aut-sei=瀬角
kn-aut-mei=美穂
aut-affil-num=1
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=2
ORCID=

en-aut-name=KinjoAkari
en-aut-sei=Kinjo
en-aut-mei=Akari
kn-aut-name=金城朱理
kn-aut-sei=金城
kn-aut-mei=朱理
aut-affil-num=3
ORCID=

en-aut-name=HidakaKei
en-aut-sei=Hidaka
en-aut-mei=Kei
kn-aut-name=日高啓
kn-aut-sei=日高
kn-aut-mei=啓
aut-affil-num=4
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=5
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=安場健一郎
kn-aut-sei=安場
kn-aut-mei=健一郎
aut-affil-num=6
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=田中義行
kn-aut-sei=田中
kn-aut-mei=義行
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Graduate school of Environmental and Life Science
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=2
en-affil=Graduate school of Environmental and Life Science
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=3
en-affil=Graduate school of Environmental and Life Science
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=4
en-affil=Asahi Kagaku Kogyo Co., Ltd.)
kn-affil=旭化学工業

affil-num=5
en-affil=Graduate school of Environmental and Life Science
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=6
en-affil=Graduate school of Environmental and Life Science
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=7
en-affil=Graduate school of Environmental and Life Science
kn-affil=岡山大学大学院　環境生命科学研究科
en-keyword=floral organs
kn-keyword=floral organs
en-keyword=Fragaria × ananassa Duch.
kn-keyword=Fragaria × ananassa Duch.
en-keyword=pistil fertility
kn-keyword=pistil fertility
en-keyword=receptacle growth
kn-keyword=receptacle growth
en-keyword=seedy fruit
kn-keyword=seedy fruit
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=1
end-page=4
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Synthetic studies of ancistrocladinium A and B, antileishmanial compounds isolated from a Congolese Ancistrocladus Species
kn-title=抗リーシュマニア原虫活性物質Ancistrocladinium AとBの合成研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Synthetic studies of ancistrocladinium A and B, antileishmanial compounds isolated from a Congolese Ancistrocladus sp., are described. Buchwald‒Hartwig coupling reaction between the dihydroisoquinoline and the naphthyl triflate failed. The main framework of ancistrocladinium A was constructed by 1,2‒addition of the amine to the naphthoquinone in the presence of celium trichloride as a catalyst. On the other hand, 1,4‒addition of the amine to the naphthoquinone proceeded without catalyst to form the framework of B. These products will be valuable leads for antileishmanial agents.
en-copyright=
kn-copyright=

en-aut-name=KiyotaHiromasa
en-aut-sei=Kiyota
en-aut-mei=Hiromasa
kn-aut-name=清田洋正
kn-aut-sei=清田
kn-aut-mei=洋正
aut-affil-num=1
ORCID=

en-aut-name=SaitoAkihito
en-aut-sei=Saito
en-aut-mei=Akihito
kn-aut-name=齋藤昭人
kn-aut-sei=齋藤
kn-aut-mei=昭人
aut-affil-num=2
ORCID=

en-aut-name=TakaiMomoko
en-aut-sei=Takai
en-aut-mei=Momoko
kn-aut-name=高井桃子
kn-aut-sei=高井
kn-aut-mei=桃子
aut-affil-num=3
ORCID=

en-aut-name=KuwaharaShigefumi
en-aut-sei=Kuwahara
en-aut-mei=Shigefumi
kn-aut-name=桑原重文
kn-aut-sei=桑原
kn-aut-mei=重文
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=2
en-affil=Graduate School of Agricultural Science, Tohoku University
kn-affil=東北大学大学農学研究科

affil-num=3
en-affil=Graduate School of Agricultural Science, Tohoku University
kn-affil=東北大学大学農学研究科

affil-num=4
en-affil=Graduate School of Agricultural Science, Tohoku University
kn-affil=東北大学大学農学研究科
en-keyword=ancistrocladinium A and B
kn-keyword=ancistrocladinium A and B
en-keyword=synthetic studies
kn-keyword=synthetic studies
en-keyword=leishmania, Ancistrocladus
kn-keyword=leishmania, Ancistrocladus
en-keyword=isoquinoline
kn-keyword=isoquinoline
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次 
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=108
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次 
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=25
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2017
kn-title=公表学術論文等リスト　2017
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=19
end-page=24
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Non-surgical Transfer Method of Elongating Bovine Conceptus
kn-title=ウシ伸長期胚の非外科的移植技術
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= 　The objectives of the present study were to establish a non-surgical transfer method for elongating bovine conceptuses and to combine this method with biopsy and sexing. Bovine conceptuses were recovered from donor cows on day 13-14 of the estrus cycle. In experiment 1, day 13 conceptuses were transferred to recipient cows using a standard day 7 embryo transfer (ET) method. The pregnancy rate of day 13 conceptus transfer (CT) is comparable to that of day 7 ET. In experiment 2, day 14 conceptuses were transferred using modified methods (balloon catheters or ET guns with modified sheaths). Using the standard ET method, no pregnancies were obtained;however, when balloon catheters or ET guns with modified sheaths were used, the pregnancy rates after CT were 48.0％ and 44.8％, respectively. In experiment 3, day 14 conceptuses were biopsied without a micromanipulator, sexed using the loop-mediated isothermal amplification (LAMP) method, and transferred to recipient cows. The pregnancy rate of biopsied conceptuses was 46.2％ and did not differ significantly from that of unbiopsied with conceptuses. Moreover, all pregnant cows transferred conceptuses following biopsy and sexing delivered calves with the expected sexes. These results suggested that the non-surgical bovine conceptus transfer (CT) method was comparable to day 7 ET, and that this technique enables biopsy and sexing without expensive equipment such as a micromanipulator or specialized skills.
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=木村康二
kn-aut-sei=木村
kn-aut-mei=康二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=応用動物科学コース
en-keyword=conceptus
kn-keyword=conceptus
en-keyword=biopsy
kn-keyword=biopsy
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=11
end-page=17
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Virus-induced gene silencing in Prunus fruit tree species with the Apple latent spherical virus vector
kn-title=リンゴ小球形潜在ウイルスベクターを用いたサクラ属果樹のウイルス 誘導性ジーンサイレンシングに 関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Virus-induced gene silencing (VIGS) has been used as a rapid and effective tool for functional analysis of genes in various plants, including woody fruit tree species. In this study, we attempted to develop a VIGS-based gene evaluation system for seven Prunus species, including apricot (P. armeniaca L.), sweet cherry (P. avium L.), almond [P. dulcis (Mill.) D. A. Webb.], peach (P. persica Batsch), Japanese apricot (P. mume Siebold & Zucc.), Japanese plum (P. salicina Lindl.), and European plum (P. domestica L.), with the Apple latent spherical virus (ALSV) vectors. ALSV vectors carrying part of the apricot PHYTOENE DESATURASE (PDS) gene sequence were amplified in Nicotiana benthamiana, and inoculated into the cotyledons of Prunus seedlings by particle bombardment. Typical PDS-silenced phenotypes, characterized by uniform discoloration of the upper leaves, were observed in sweet cherry and some cultivars of apricot and almond several weeks after inoculation. In contrast, attempted ALSV infections of Japanese apricot, Japanese plum, European plum, and the other cultivars of apricot and almond were unsuccessful. Furthermore, although the infection rate of ALSV in peach was high, severe viral infection symptoms were observed in the infected leaves. These results collectively suggested that the efficiency of ALSV infection and VIGS could vary depending on species and/or cultivar in Prunus. The possible use of the ALSV-mediated VIGS system for functional analysis of genes in Prunus is discussed.
en-copyright=
kn-copyright=

en-aut-name=KawaiTakashi
en-aut-sei=Kawai
en-aut-mei=Takashi
kn-aut-name=河井崇
kn-aut-sei=河井
kn-aut-mei=崇
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=gene evaluation system
kn-keyword=gene evaluation system
en-keyword=post-transcriptional gene silencing
kn-keyword=post-transcriptional gene silencing
en-keyword=virus vector
kn-keyword=virus vector
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=5
end-page=10
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Alzheimer’s disease : Relationship between the Alzheimer’s disease and human microbiome
kn-title=アルツハイマー病の背景と発症 : ヒトマイクロバイオーム（細菌叢）との関連の視点から
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Alzheimer’s disease (AD) is a progressive, neurodegenerative disease characterized by memory and language disorder. The accumulation of senile plaques called β-amyloid and neurofibrillary tangles involving protein tau in the brains of AD patients have been considered as two hallmarks of AD. In AD, it is reported that accumulation of β-amyloid may be observed 25 years before onset, supporting early diagnosis and treatment by brain image analysis, because several techniques have recently been developed to detect β-amyloid and tau protein in brains of persons diagnosed with AD. AD patients are usually suffering from other diseases such as diabetes or periodontal disease, and there is accumulating data to show that these diseases associate with the human microbiome, such as gut and oral microbiota. In this report, the relation ship between AD and the human microbiome is reviewed.
en-copyright=
kn-copyright=

en-aut-name=FujiiYusuke
en-aut-sei=Fujii
en-aut-mei=Yusuke
kn-aut-name=藤井祐介
kn-aut-sei=藤井
kn-aut-mei=祐介
aut-affil-num=1
ORCID=

en-aut-name=MoritaHidetoshi
en-aut-sei=Morita
en-aut-mei=Hidetoshi
kn-aut-name=森田英利
kn-aut-sei=森田
kn-aut-mei=英利
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院　環境生命科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院　環境生命科学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=1
end-page=3
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Olibanum and Tea-tree, used in Aromatherapy today, on the Body and Mind
kn-title=現代のアロマセラピーで使用する乳香とティートリー精油の 身体・心への影響について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Olibanum (frankincense), used as a religious offering, has been one of the most valuable and
respected substances since ancient times. Tea-tree, with a long history as a folk medicine for Australian aborigines, has been used in modern aromatherapy since the late 1970’s. In this paper, comparisons of olibanum and tea-tree in aromatherapy and their effects on the body and mind are discussed.
en-copyright=
kn-copyright=

en-aut-name=AsadaaMasako
en-aut-sei=Asadaa
en-aut-mei=Masako
kn-aut-name=浅田昌古
kn-aut-sei=浅田
kn-aut-mei=昌古
aut-affil-num=1
ORCID=

en-aut-name=SakaiTakashi
en-aut-sei=Sakai
en-aut-mei=Takashi
kn-aut-name=酒井貴志
kn-aut-sei=酒井
kn-aut-mei=貴志
aut-affil-num=2
ORCID=

en-aut-name=KiyotaHiromasa
en-aut-sei=Kiyota
en-aut-mei=Hiromasa
kn-aut-name=清田洋正
kn-aut-sei=清田
kn-aut-mei=洋正
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=放送大学

affil-num=2
en-affil=
kn-affil=岡山大学名誉教授

affil-num=3
en-affil=
kn-affil=岡山大学大学院　環境生命科学研究科
en-keyword=flavor and fragrance
kn-keyword=flavor and fragrance
en-keyword=frankincense
kn-keyword=frankincense
en-keyword=aromatherapy
kn-keyword=aromatherapy
en-keyword=monoterpenoids
kn-keyword=monoterpenoids
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=107
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=59
end-page=73
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2016.
kn-title=公表学術論文等リスト　2016
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=51
end-page=57
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Expansion of large-scale Paddy field management entity in the regions advanced in agricultural structure
kn-title=農業構造変動の進展地域における 大規模水田農業経営の展開
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　This paper analyzes expansion of large-scale Paddy field management entity and change of agricultural structure in Japan. Paddy agriculture has changed. Mid-size agricultural management entities have decreased and large-scale management entities have increased. This factor is due to downturn in rice price and agricultural structure policy. Those large-scale management entities have accumulated paddy fields more than half of agricultural commune. Sustainability and vison of such a farm management entities are vital for maintenance and development of regional agriculture.<br>
　This paper takes up development of large-scale management. N Corporation, which manages 395ha of farmland in Toyota, Aichi Prefecture, has been analyzed as a case study. The findings of the analysis are as follows : The speed of increasing the workforce to accompany the enlargement of the managed area depends on, which is the reason why N Corporation became organization change from cooperative to corporation. The conversion in the latter case has changed the status of workers, who used to be members of the cooperative, to employees and the management of workers from a horizontal system to a more vertical and sectional structure.<br>
　From the above, this paper can draw the following conclusion : Large-scale management entities was changed by field scale expansion. They are face a decrease in yield and decrease in earnings of the paddy fields agriculture. As a result, they need to organization change. And agricultural structure policy is not only required to accumulate fields but also to overall management support.
en-copyright=
kn-copyright=

en-aut-name=OhanakaKatsutoshi
en-aut-sei=Ohanaka
en-aut-mei=Katsutoshi
kn-aut-name=大仲克俊
kn-aut-sei=大仲
kn-aut-mei=克俊
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=tenanted land
kn-keyword=tenanted land
en-keyword=agricultural cooperatives
kn-keyword=agricultural cooperatives
en-keyword=large-scale management entity
kn-keyword=large-scale management entity
en-keyword=enlargement
kn-keyword=enlargement
en-keyword=organization change
kn-keyword=organization change
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=43
end-page=49
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fruit quality control using water management and simple water status detection methods in fruit tree leaves
kn-title=果樹における水分ストレスによる 果実成分の制御と葉内水分の計測
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　In fruit production, water status during the maturation season has a significant effect on fruit quality, influencing not only sugar but also organic acid and amino acid contents. Water management technology, therefore, is important for improving fruit quality and maintaining yield in Japan. This study firstly discusses extensive research into the effect of irrigation control on each component of grape fruit juice. Secondly, given that real time monitoring of leaf moisture content is essential to controlling water status, a simple estimation method is described. This method involved fixing a “water stress indicator” to the abaxial side of a leaf; the indicator changed color depending on the water status, which could then be evaluated. The water status was assessed against an indicator color scale, based on a property of cobalt （II） chloride that causes it to change color, from blue to light pink, through a hydration reaction between the indicator sheet and the water evaporating from the leaf to which the indicator sheet is affixed. By using this method, estimates were made of decreases under water stress conditions in the water evaporation rate from satsuma mandarin, grapes, peaches, and Japanese pear, based on the time required for the indicator sheet to change color. Thirdly, a new electrical sensor method to investigate water status in fruit tree leaves was developed, and used to measure electro pulse period; the relationship with transpiration rate was then evaluated using a leaf porometer. Pulse period was found to be consistently correlated with transpiration rate. The results indicate that the water status of fruit tree leaves can be estimated by measuring pulse period. This provides an accurate and quick method for detecting water stress, which could potentially be used for other crops that are particularly sensitive to water stress.
en-copyright=
kn-copyright=

en-aut-name=MorinagaKunihisa
en-aut-sei=Morinaga
en-aut-mei=Kunihisa
kn-aut-name=森永邦久
kn-aut-sei=森永
kn-aut-mei=邦久
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=fruit quality
kn-keyword=fruit quality
en-keyword=water status
kn-keyword=water status
en-keyword=patch test
kn-keyword=patch test
en-keyword=pulse period
kn-keyword=pulse period
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=39
end-page=42
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Mitochondrial dynamics in mammalian oocytes
kn-title=哺乳類卵子におけるミトコンドリアの動態
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Subcellular distribution of mitochondria are reorganized during development of the oocyte into a
fertilizable egg. In growing oocytes, mitochondria are heterogeneously distributed in the cytoplasm, whereas they diffuse throughout the cytoplasm in fully-grown oocytes. GFP-labeled mitochondria demonstrate that oocyte maturation involves dynamic redistribution of the mitochondria, whose process is associated with microtubule organization. These spatio-temporal regulations of mitochondria in oocytes may be an important process in the preparation for fertilization and subsequent embryonic development.
en-copyright=
kn-copyright=

en-aut-name=WakaiTakuya
en-aut-sei=Wakai
en-aut-mei=Takuya
kn-aut-name=若井拓哉
kn-aut-sei=若井
kn-aut-mei=拓哉
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=Oocyte
kn-keyword=Oocyte
en-keyword=Mitochondria
kn-keyword=Mitochondria
en-keyword=Meiosis
kn-keyword=Meiosis
en-keyword=Cytoskeleton
kn-keyword=Cytoskeleton
en-keyword=Endoplasmic reticulum
kn-keyword=Endoplasmic reticulum
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=27
end-page=32
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=トウガラシ（Capsicum baccatum）における カプサイシノイド含量の変異とその非辛味系統
kn-title=Variations in capsaicinoid contents in the chili pepper (Capsicum baccatum) and its non-pungent accessions
en-subtitle=
kn-subtitle=
en-abstract=　トウガラシ（Capsicum 属）は世界的に重要な香辛料および野菜である．C. baccatum は南米原産のマイナーな栽培種であるが，果色，果形や辛味など果実形質に多様性が認められることから，トウガラシ遺伝資源として注目されている．トウガラシの辛味性についてはC. annuum 種において多くの研究が行われているが，C. baccatum 種においては十分研究されていない．<br>
　本研究では，C. baccatum 36系統について辛味成分カプサイシノイドの含量を調査した．カプサイシノイド含量の幅は0 ～4,258 ㎍ /gDW であった．また果実重と辛味成分含量の間に負の相関が認められた．C. baccatum の辛味は低〜中程度であるが，非辛味系統はほとんど認められず，唯一1 系統（‘Kaleidoscope’）が非辛味であった．この非辛味の安定性を調査するために，辛味程度の異なる系統とともに異なる収穫時期におけるカプサイシノイド含量を調査した．<br>
　他のC. baccatum 系統ではカプサイシノイド含量は収穫時期で変化したが，‘Kaleidoscope’ ではいずれの収穫時期でもカプサイシノイドは検出されなかった．本研究で見出された非辛味系統は将来のC. baccatum の育種において有用であろう．
kn-abstract=The chili pepper (Capsicum) is both an important spice and fresh vegetable worldwide. C. baccatum is a lesser known domesticated species that is native to the Andean region. Fruit traits such as color, shape, and pungency markedly vary in this species. C. baccatum has potential as a bioresource for future chili pepper breeding programs. Although extensive studies have been conducted on the pungency of C. annuum, that of C. baccatum has not been examined in as much detail. In the present study, capsaicinoid contents were analyzed in 36 C. baccatum accessions. Capsaicinoid contents ranged between 0 and 4,258 μg/gDW. Furthermore, a negative relationship was observed between capsaicinoid contents and fruit weights. Although the pungency of C. baccatum is regarded as low-mild, very few non-pungent accessions were detected ; only one non-pungent accession (‘Kaleidoscope’) was identified among the C. baccatum accessions examined. In order to validate the stability of non-pungency in the accession, capsaicinoid contents were determined at different harvest dates, along with other accessions with different pungencies. Although capsaicinoid contents in other C. baccatum accessions changed with the picking date, capsaicinoid was not detected in ‘Kaleidoscope’ at any date. The non-pungent accession reported here may be useful for future C. baccatum pepper breeding programs.
en-copyright=
kn-copyright=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=田中義行
kn-aut-sei=田中
kn-aut-mei=義行
aut-affil-num=1
ORCID=

en-aut-name=HaraMotohito
en-aut-sei=Hara
en-aut-mei=Motohito
kn-aut-name=原一仁
kn-aut-sei=原
kn-aut-mei=一仁
aut-affil-num=2
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=3
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=4
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=安場健一郎
kn-aut-sei=安場
kn-aut-mei=健一郎
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=3
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=4
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=5
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=Bio-resource
kn-keyword=Bio-resource
en-keyword=Fruit shape
kn-keyword=Fruit shape
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=21
end-page=25
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Beginning Date of Intermittent Low Temperature Treatments on Flowering of Tray-grown Strawberry 'Nyoho'
kn-title=処理開始時期がトレイ育苗したイチゴ‘女峰’に対する 間欠冷蔵処理の効果発現に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Intermittent low temperature storage is expected to be a new artificial flower-inducing treatment and becoming to be a practical procedure as it does not require expensive equipment or much energy cost. Tray-grown ‘Nyoho’ plants were placed in a refrigerator (15°C, in the dark) for 3 days and then transferred to a further outdoor shelter for 3 days. Plants were transferred at noon and this cycle was repeated twice (3D/3D). Such 3D/3D treatments were begun on August 22, 25, 28, 31, and September 3and the effectiveness on flowering was determined for 2 years. Both in 2012 and 2013, treatments begun before August 28 were less effective compared to the treatments begun on August 28 or later. Continuous 6 days of 15°C storage (6D) was effective to some extent, but 6D from August 25 was apparently less effective compared to those begun later. Both in intermittent and continuous treatments for ‘Nyoho’, it was confirmed that any flower-inducing effect became relatively small and unstable when the treatments were begun before 28 August in Okayama.
en-copyright=
kn-copyright=

en-aut-name=KinjoaAkari
en-aut-sei=Kinjoa
en-aut-mei=Akari
kn-aut-name=金城朱理
kn-aut-sei=金城
kn-aut-mei=朱理
aut-affil-num=1
ORCID=

en-aut-name=HanadaAtsushi
en-aut-sei=Hanada
en-aut-mei=Atsushi
kn-aut-name=花田惇史
kn-aut-sei=花田
kn-aut-mei=惇史
aut-affil-num=2
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=3
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=4
ORCID=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=安場健一郎
kn-aut-sei=安場
kn-aut-mei=健一郎
aut-affil-num=5
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=田中義行
kn-aut-sei=田中
kn-aut-mei=義行
aut-affil-num=6
ORCID=

en-aut-name=YoshidaAtsuko
en-aut-sei=Yoshida
en-aut-mei=Atsuko
kn-aut-name=吉田敦子
kn-aut-sei=吉田
kn-aut-mei=敦子
aut-affil-num=7
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=3
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=4
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=5
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=6
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=7
en-affil=Nozomi Farm Co.
kn-affil=(有)のぞみふぁーむ
en-keyword=artificial flower induction
kn-keyword=artificial flower induction
en-keyword=Fragaria x ananassa Duch.
kn-keyword=Fragaria x ananassa Duch.
en-keyword=low temperature storage
kn-keyword=low temperature storage
en-keyword=runner cutting
kn-keyword=runner cutting
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=13
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=圃場栽培したベトナム産水稲品種（Oriza sativa L.）の乾物生産，収量と玄米の外観品質
kn-title=Effect of shading on dry-matter production, yield and grain appearance quality of Vietnamese rice cultivars （Oryza sativa L.） in the paddy field
en-subtitle=
kn-subtitle=
en-abstract=　光強度は，水稲の生育・収量を決定する最も重要な環境要因である．しかし，光強度の減少，すなわち遮光処理がインド型水稲品種，特にベトナム産品種の生育・収量に及ぼす影響に関する知見は限られている．本研究では，登熟期間の遮光がベトナム産水稲品種の乾物生産，収量と収量構成要素，玄米の外観品質に及ぼす影響を2015年に調査した．ベトナム産水稲14品種を圃場栽培を行い，最初に出穂が認められた品種の出穂期以降収穫期まで，群落上層を黒色寒冷紗（遮光率50 ）を用いて被覆する，遮光処理を行った．ベトナム産14品種の収量は，遮光に伴い自然光条件に比較して32.2～65.0 の低下がみられた．遮光処理の影響が大きかった品種は‘Jasmine 85’ と‘OM2395’ で，それぞれ65.0，59.8 の収量低下がみられた．収量の低下には，登熟期の個体群成長速度，収量構成要素では穎花不稔の増加による登熟歩合の低下に起因していた．遮光処理はすべての品種で玄米の未熟粒割合を増加させた．遮光処理により，白未熟粒と青未熟粒の増加がみられ，その程度は白未熟粒が‘OM2517’ で，青未熟粒が‘OM5415’ で最も著しかった．以上の結果，遮光処理はベトナム産水稲14品種の収量と玄米の外観品質を低下させたが，その程度には品種間差があることが明らかになり，これらの傾向は日本産水稲品種で得られた結果と同様であった．
kn-abstract=Light intensity is one of the most important environmental factors to determine the growth and yield of rice. However, little information is known about the response of indica cultivars, especially Vietnamese cultivars to shading conditions. In this study, a field experiment was conducted in 2015 to determine the response of Vietnamese cultivars to shading condition during the grain filling period. Fourteen Vietnamese cultivars were covered by black cloth (50% shading treatment) after the heading stage in the field conditions. Grain yield of fourteen Vietnamese cultivars in shading condition fell to about 32.2–65.0% of the natural light condition. The most sensitive cultivars to shading are ‘Jasmine 85’ and ‘OM2395’, of which the grain yield decreased to 65.0 and 59.8% of natural light condition, respectively. The shaded yield decreased corresponding to the reduction in crop growth rate (CGR) and yield components, especially in the percentage of filled grain due to the increase in spikelet sterility. Shading increased the percentage of immature grains in all cultivars. Shading induced an increase in the percentage of chalky and green grains, which was largest in ‘OM2517’ and ‘OM5451’, respectively. Clearly, shading depressed both grain yield and the appearance quality of brown rice in Vietnamese cultivars, similarly reported in previous studies with Japanese cultivars.
en-copyright=
kn-copyright=

en-aut-name=Tran LocThuya
en-aut-sei=Tran LocThuya
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=Crop growth rate
kn-keyword=Crop growth rate
en-keyword=Dry matter production
kn-keyword=Dry matter production
en-keyword=Grain appearance quality
kn-keyword=Grain appearance quality
en-keyword=Shading
kn-keyword=Shading
en-keyword=Vietnamese cultivars.
kn-keyword=Vietnamese cultivars.
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=5
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ginkgo biloba α-fucosidase with activity towards plant complex type N-glycans containing the Lewis a epitope: Purification and characterization
en-subtitle=
kn-subtitle=
en-abstract=　銀杏種子から高分子量 （SDS-PAGE で120 kDa） を有し，α-フコース含有オリゴ糖に活性を示すα-フコシダーゼ（α-fucosidase Gb）を均一に精製した．ルイス a エピトープ含有 N-グリカンを基質とした場合，α-fucosidase Gb の至適 pH は 5.5 付近であることから，本酵素は液胞のような酸性環境で機能していることが示唆された．N‒末端アミノ酸配列が化学修飾のため同定できなかったため，本酵素が GH29 ファミリーに属するかどうかは不明である．α-Fucosidase Gb は，Lacto-N-fucopentaose IIIの α1,3-フコース残基やルイス a エピトープ含有の植物複合型N-グリカンのα1,4-フコース残基を加水分解することから，α-フコース含有オリゴ糖やN 型糖タンパク質の分解プロセスに関与することが示唆された．
kn-abstract=　We have identified, and purified to homogeneity, a high molecular weight Ginkgo biloba α-fucosidase (α-fucosidase Gb, 120 kDa estimated by SDS‒PAGE) with activity against α-fucosylated oligosaccharides. When a Lewis a epitope-containing N-glycan was used as a substrate, α-fucosidase Gb showed optimum activity at approximately pH 5.5, suggesting that it functions in acidic environments such as the vacuole. It remains uncertain, however, whether this Ginkgo α-fucosidase belongs to the GH29 family, since its N-terminal sequence could not be determined, probably due to a chemical modification. α-Fucosidase Gb showed substantial activity towards the α1,3-fucosyl linkage in Lacto-N-fucopentaose III and an α1,4-fucosyl linkage in the Lewis a epitope found in plant complex type N-glycans, indicating an involvement in the degradation process of α-fucosylated oligosaccharides or N-glycoproteins.
en-copyright=
kn-copyright=

en-aut-name=ItanoSatsuki
en-aut-sei=Itano
en-aut-mei=Satsuki
kn-aut-name=板野紗月
kn-aut-sei=板野
kn-aut-mei=紗月
aut-affil-num=1
ORCID=

en-aut-name=MaedaMegumi
en-aut-sei=Maeda
en-aut-mei=Megumi
kn-aut-name=前田恵
kn-aut-sei=前田
kn-aut-mei=恵
aut-affil-num=2
ORCID=

en-aut-name=Md. Ziaur Rahman
en-aut-sei=Md. Ziaur Rahman
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KimuraYoshinobu
en-aut-sei=Kimura
en-aut-mei=Yoshinobu
kn-aut-name=木村吉伸
kn-aut-sei=木村
kn-aut-mei=吉伸
aut-affil-num=4
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=3
en-affil=Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission
kn-affil=

affil-num=4
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=α-fucosidase
kn-keyword=α-fucosidase
en-keyword=plant N-glycan
kn-keyword=plant N-glycan
en-keyword=N-glycan degradation
kn-keyword=N-glycan degradation
en-keyword=Ginkgo biloba
kn-keyword=Ginkgo biloba
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=1
end-page=3
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Compulsory Repetitive Learning to Improve Student Achievement and Performance in Fundamental Subjects
kn-title=半強制的な反復学習による基礎科目の徹底教育
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　We employ the methodology of “Compulsory Repetitive Learning” to improve student learning and performance in fundamental subjects. It is a challenge for many universities to intrinsically motivate students and it is a demanding issue that a large part of students do not sufficiently do homework. Here, we let students solve the problems associated with the teaching immediately following each lecture. Through the usage of “Compulsory Repetitive Learning”, each student as well as the subject teacher(s) could monitor and evaluate the quality of student learning and performance.
en-copyright=
kn-copyright=

en-aut-name=IzumiMinoru
en-aut-sei=Izumi
en-aut-mei=Minoru
kn-aut-name=泉実
kn-aut-sei=泉
kn-aut-mei=実
aut-affil-num=1
ORCID=

en-aut-name=MurataYoshiyuki
en-aut-sei=Murata
en-aut-mei=Yoshiyuki
kn-aut-name=村田芳行
kn-aut-sei=村田
kn-aut-mei=芳行
aut-affil-num=2
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=Graduate School of Environmental and life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=compulsory repetitive learning
kn-keyword=compulsory repetitive learning
en-keyword=homework
kn-keyword=homework
en-keyword=exercises
kn-keyword=exercises
en-keyword=learning comprehension level
kn-keyword=learning comprehension level
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=106
cd-vols=
no-issue=
article-no=
start-page=33
end-page=38
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=20170201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Enzyme-catalyzed synthesis and odor evaluation of both enantiomers of perfume compounds
kn-title=酵素触媒反応を鍵とする光学活性な香気物質両鏡像体の合成と香気評価
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Synthesis of both enantiomers of some perfume compounds, and their difference in aroma
characteristics are described. Enantiomeric pairs of methyl jasmonate and its 4,5-didehydro congener, principal components of jasmin absolute, were prepared from the corresponding commercially available racemates using lipase-catalyzed optical resolution. The E-value for the reaction is as high as 370. The nature-identical isomers produced superior aroma activity relative to unnatural ones. Racemic lavandulol from a commercial source, was also resolved using several enzymatic transesterifications followed by hydrolysis with PPL. Odor evaluation revealed that the nature-identical isomer should play a key role in lavender oil. Cis-α-irone and cis-γ-irone, used as important violet components for perfumery, were synthesized in optically active forms through fractional crystallization of the diastereomeric salts with α-phenethylamine. The nature-identical irones also had better floral characteristics like ionone.
en-copyright=
kn-copyright=

en-aut-name=KiyotaHiromasa
en-aut-sei=Kiyota
en-aut-mei=Hiromasa
kn-aut-name=清田洋正
kn-aut-sei=清田
kn-aut-mei=洋正
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=岡山大学大学院環境生命科学研究科
en-keyword=flavor and fragrance
kn-keyword=flavor and fragrance
en-keyword=enantiomers
kn-keyword=enantiomers
en-keyword=synthesis
kn-keyword=synthesis
en-keyword=methyl jasmonate
kn-keyword=methyl jasmonate
en-keyword=lavandulol
kn-keyword=lavandulol
en-keyword=irone
kn-keyword=irone
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=41
end-page=52
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2015.
kn-title=公表学術論文等リスト　2015
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=35
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Identification of commensal bacteria coated with secretory immunoglobulin A
kn-title=分泌型免疫グロブリンＡが認識する 腸内細菌種の同定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　A part of commensal intestinal bacteria in mammal including human, mouse, bovine and pig are coated with secretory immunoglobulin A (S‒IgA). It has been suggested from our previous research that S‒IgA coating of commensal bacteria occur in bacterial group specific manner in human and mouse intestine. Thus, identification of S‒IgA‒coated bacterial genera/species would certainly help to elucidate the interaction between S‒IgA and commensal intestinal bacteria. However, the method to identify the genera/species of S‒IgA‒coated bacteria has not been established. To identify S‒IgA‒coated bacterial composition, we developed the method combining immunohistochemical detection of S‒IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Furthermore, human and mice fecal S‒IgA coated bacterial composition was evaluated by this newly developed method with ten frequently‒used FISH probes. Fecal S‒IgA‒coated bacterial composition was successfully analyzed with this method and this analysis suggested that Enterobacteriaceae was preferably coated with S‒IgA whereas Bacteroides/Prevotella and Lactobacillus/ Enterococcus groups seemed to be poorly coated with S‒IgA. This method will be applied to confirm whether interaction between S‒IgA and commensal intestinal bacteria relate to symptom of inflammatory bowel diseases.
en-copyright=
kn-copyright=

en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=鶴田剛司
kn-aut-sei=鶴田
kn-aut-mei=剛司
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=Secretory immunoglobulin A
kn-keyword=Secretory immunoglobulin A
en-keyword=commensal intestinal bacteria
kn-keyword=commensal intestinal bacteria
en-keyword=fluorescence in situ hybridization
kn-keyword=fluorescence in situ hybridization
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=29
end-page=33
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Regulatory mechanisms for the expressions of local factors controlling bovine oviductal smooth muscle motility
kn-title=ウシ卵管平滑筋運動を制御する局所 因子とその発現制御機構
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Oviductal motility is required for transport of oocyte and embryo resulting in successful fertilization and implantation in mammals. The oviduct consists of epithelial, stromal and smooth muscle layers. Oviductal motility is systemically and locally regulated by various factors including prostaglandin F2 alpha (PGF) and endothelins (EDNs), and relaxing factors including prostaglandin E2 (PGE2) and nitric oxide (NO). The objective of our research is to clarify the regulatory system of oviductal motility including the production mechanisms of these factors in cattle. First, the expressions of regulating factors of oviductal motility were examined throughout the estrous cycle in the bovine oviduct. Some of them showed cyclical changes, which suggested that they were controlled by some other factors. Second, the effects of ovarian steroids or oviductal local factors on the expressions of PGs, EDNs and NO synthases were investigated using cell culture method. Several factors such as estradiol‒17beta, progesterone and lysophosphatidic acid affected the expressions of regulating factors of smooth muscle motility. In addition, we found that these actions differed between the ampulla and isthmus in same types of cultured cell. Our studies suggest that regulatory factors of oviductal motility are produced during the optimal period and at proper location to transport the oocyte and early embryo in the bovine oviduct. Although the precise control of oviductal motility is essential for successful pregnancy, methods for diagnosing and treating of its functional abnormality have not been established yet not only in cows but also in other animals including human. Our studies should contribute to improving the fertility rates in mammals.
en-copyright=
kn-copyright=

en-aut-name=YamamotoYuki
en-aut-sei=Yamamoto
en-aut-mei=Yuki
kn-aut-name=山本ゆき
kn-aut-sei=山本
kn-aut-mei=ゆき
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=ovarian steroid
kn-keyword=ovarian steroid
en-keyword=oviduct
kn-keyword=oviduct
en-keyword=prostaglandin
kn-keyword=prostaglandin
en-keyword=physiology
kn-keyword=physiology
en-keyword=smooth muscle motility
kn-keyword=smooth muscle motility
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=21
end-page=27
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Functional analysis of plant immune regulator OsPti1a in rice
kn-title=イネ耐病性制御因子OsPti1a の 制御機構の解明
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　An understanding of plant immune systems is important for crop breeding with enhanced disease resistance against pathogen infection. Previous studies reveal that plant has evolved two types of defense mechanisms, which are called “basal resistance” and “R‒gene mediated resistance”, for protecting thewrselves from pathogen attack. Recent studies suggest that both defense systems use a common pathway to activate defense responses, however, the downstream components in both pathways are still obscure. 
　OsPto-interacting protein 1a (OsPti1a), which is a functional ortholog of tomato Pti1, negatively regulates both basal resistance and R‒gene mediated resistance in rice. ospti1a mutant shows lesion formation and accompanying defense responses without pathogen infection. OsPti1a is phosphorylated by upstream kinase oxidative signal inducible1 (OsOxi1) and the phosphorylation of OsPti1a has an important role in activating basal resistance against pathogen infection. Additionally, OsOxi1 is phosphorylated by upstream kinase 3‒phosphoinotiside-dependent protein kinase 1 (OsPdk1). OsPdk1 has an important role for activating basal resistance against compatible pathogen infection. Therefore, OsPdk1-OsOxi1-OsPti1a phosphorylation cascade regulates proper activation of basal resistance in rice.
　Interestingly, OsPti1a localizes at plasma membrane, and cellular localization of OsPti1a has an important function in suppvessing lesion formation. Especially, N‒terminal amino acid sequences of OsPti1a have a post-translational modification for binding to plasma membrane. Further, OsPti1a forms complexes with potentially plant immune related proteins at plasma membrane, suggesting that plasma membrane localized OsPti1a probably regulates plant immune complex through its phosphorylation during pathogen infection.
en-copyright=
kn-copyright=

en-aut-name=MatsuiHidenori
en-aut-sei=Matsui
en-aut-mei=Hidenori
kn-aut-name=松井英譲
kn-aut-sei=松井
kn-aut-mei=英譲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=lesion mimic mutant
kn-keyword=lesion mimic mutant
en-keyword=plant immunity
kn-keyword=plant immunity
en-keyword=phosphorylation
kn-keyword=phosphorylation
en-keyword=signaling
kn-keyword=signaling
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=17
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental study on identification of target molecule of isothiocyanates using stable conjugation with lysine residue
kn-title=リジン付加反応を利用したイソチオ シアネート類の標的分子探索のため の基礎的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Isothiocyanates (ITCs) are contained as glucosinolates in cruciferous plants such as Wasabia japonica ( wasabi) and broccoli. Numerous studies have shown that ITCs have beneficial effects in our body such as induction of detoxification enzymes and inhibitory effects on cancer cell growth. The biological activities of ITCs are considered to be triggered in the reaction of ITC with thiols to form an unstable thiocarbamoyl adduct. On the other hand, ITCs are also known to react with amino moieties stably under alkaline pH. However, the reaction of ITCs with amino moieties under physiological conditions has not been explored fully. Therefore, we investigated the reactivity of allyl ITC (AITC) with amino groups under neutral conditions. When AITC was incubated with bovine serum albumin (BSA) as a model protein in a phosphate buffer (pH 7.4), amino groups were decreased. In addition, AITCmodified Nα‒benzoyl-glycyl-L-lysine (BGK) with a Nε‒thiocarbamoyl linkage was detected by incubation of AITC and BGK in the buffer. To verify the transformation of ITC from predominant target ‘thiol’ to amine, synthetic AITC‒modified Nα‒acetyl-L-cysteine (NAC) was incubated with BGK. The AITC‒Lys adduct was generated in a timedependent manner, while AITC‒NAC adduct was degraded. Furthermore, AITC‒Lys adduct was detected from the mixture of AITC‒NAC and BSA using a novel anti‒AITC‒Lys monoclonal antibody. Thus, the adduct of ITC and Lys residue may be a useful tag for identification of ITC target molecules.
en-copyright=
kn-copyright=

en-aut-name=NakamuraToshiyuki
en-aut-sei=Nakamura
en-aut-mei=Toshiyuki
kn-aut-name=中村俊之
kn-aut-sei=中村
kn-aut-mei=俊之
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=isothiocyanate
kn-keyword=isothiocyanate
en-keyword=lysine
kn-keyword=lysine
en-keyword=cysteine
kn-keyword=cysteine
en-keyword=reactivity
kn-keyword=reactivity
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=7
end-page=15
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=分子遺伝学的手法を用いたわが国メロン品種の多様性と分類
kn-title=Molecular-based analysis of genetic diversity and classification of Japanese melon breeding lines
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=For the breeding of Japanese netted melon, various types of foreign cultivars have been utilized for improving adaptability, disease and pest resistance, fruit quality and so on. However, little is known about their genetic diversity and relationships, since most cultivars derived from crosses between various horticultural groups. To figure out the genetic structure of Japanese melon, in this study, 57 melon accessions from three horticultural groups were examined using 55 RAPD markers produced by 24 RAPD primers. Genetic diversity of the Japanese netted melon was as high as those of cultivar groups of Groups Cantalupensis and Inodorus, while it was low in Group Conomon irrespective of large variations in fruit traits. Cluster analysis and PCO analysis based on genetic distance showed that Group Conomon was distantly related to other melon accessions. Among the latter, European cantaloupe (nonnetted) and American open-field type (netted) proved to be genetically close, while England glasshouse melon (netted) including ‘Earl’s Favourite’ is distantly related to these two groups and closely related with Group Inodorus. It was therefore suggested that England glasshouse type was established from hybrids between European cantaloupe and Group Inodorus. Japanese netted melon was most closely related with England glasshouse type, irrespective of the fact that various kinds of melon accessions have been crossed to improve adaptability, disease resistance and so on. In contrast, pure line cultivars of the Japanese netted melon bred by pure line selection from ‘Earl's Favourite’ or by crossing ‘Earl’s Favourite’ with ‘British Queen’ were confirmed to be mostly homogenous, and it was difficult to establish RAPD markers to discriminate each cultivar. Group Conomon var. makuwa and var. conomon, which have been cultivated and utilized as different crops, proved to be genetically indistinguishable and were considered to share the same gene pool.
en-copyright=
kn-copyright=

en-aut-name=DungTran Phuong
en-aut-sei=Dung
en-aut-mei=Tran Phuong
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TanakaKatsunori
en-aut-sei=Tanaka
en-aut-mei=Katsunori
kn-aut-name=田中克典
kn-aut-sei=田中
kn-aut-mei=克典
aut-affil-num=2
ORCID=

en-aut-name=AkashiYukari
en-aut-sei=Akashi
en-aut-mei=Yukari
kn-aut-name=明石由香利
kn-aut-sei=明石
kn-aut-mei=由香利
aut-affil-num=3
ORCID=

en-aut-name=ThuyDuong Thanh
en-aut-sei=Thuy
en-aut-mei=Duong Thanh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NishidaHidetaka
en-aut-sei=Nishida
en-aut-mei=Hidetaka
kn-aut-name=西田英隆
kn-aut-sei=西田
kn-aut-mei=英隆
aut-affil-num=5
ORCID=

en-aut-name=KatoKenji
en-aut-sei=Kato
en-aut-mei=Kenji
kn-aut-name=加藤鎌司
kn-aut-sei=加藤
kn-aut-mei=鎌司
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学環境生命科学研究科

affil-num=2
en-affil=
kn-affil=弘前大学人文学部

affil-num=3
en-affil=
kn-affil=岡山大学環境生命科学研究科

affil-num=4
en-affil=
kn-affil=岡山大学環境生命科学研究科

affil-num=5
en-affil=
kn-affil=岡山大学環境生命科学研究科

affil-num=6
en-affil=
kn-affil=岡山大学環境生命科学研究科
en-keyword=breeding
kn-keyword=breeding
en-keyword=classification
kn-keyword=classification
en-keyword=genetic diversity
kn-keyword=genetic diversity
en-keyword=melon
kn-keyword=melon
en-keyword=RAPD
kn-keyword=RAPD
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=1
end-page=5
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Survey on the undernourished university students who tend to lack breakfast ; a proposal for a novel viewpoint for the improvement
kn-title=大学生の朝食欠食習慣の統計解析と改善への新指針
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study investigated the current status and causes underneath the life of university students who tend to lack breakfast at a relatively high frequency, and statistical analysis on consequences leading to such lack of well-nourished eating habitat in their university life. In October 2014, self-assessed questionnaires were administered to over 150 faculty students. It contained questions about breakfast habits, time allowance for the morning class, and lunchtime setting in their high school timetable. Breakfast states were clearly separated in three groups : 68% of students regularly have breakfast throughout the weekdays, 21% students skipping the breakfast occasionally, and 11% student no habit for breakfast at all. The survey on the high school lives revealed that 70% students used to have lunch 30 min later than the lunchtime set in the university timetable, 7% of them had the lunch time even more than 1 h later. Lunchtime varies among high schools, and statistical significance was revealed (p<0.01) that schools with higher deviation scores tend have late lunch beyond 12: 30. Accordingly, university students were given directions to prepare for the timetable reform on postulation of having lunch time over one o’clock. After continuous survey on the breakfast habits during the second semester, more than 90% of students established the habit of breakfast regularly in their university lives with the improved consciousness toward well-balanced healthy breakfast contents for their higher level of education quality.
en-copyright=
kn-copyright=

en-aut-name=TamuraT.
en-aut-sei=Tamura
en-aut-mei=T.
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=1
ORCID=

en-aut-name=IbiT.
en-aut-sei=Ibi
en-aut-mei=T.
kn-aut-name=揖斐隆之
kn-aut-sei=揖斐
kn-aut-mei=隆之
aut-affil-num=2
ORCID=

en-aut-name=InagakiK.
en-aut-sei=Inagaki
en-aut-mei=K.
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=3
ORCID=

en-aut-name=KuboY.
en-aut-sei=Kubo
en-aut-mei=Y.
kn-aut-name=久保康隆
kn-aut-sei=久保
kn-aut-mei=康隆
aut-affil-num=4
ORCID=

en-aut-name=OkudaK.
en-aut-sei=Okuda
en-aut-mei=K.
kn-aut-name=奥田潔
kn-aut-sei=奥田
kn-aut-mei=潔
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学教務FD 委員会

affil-num=2
en-affil=
kn-affil=岡山大学教務FD 委員会

affil-num=3
en-affil=
kn-affil=岡山大学教務FD 委員会

affil-num=4
en-affil=
kn-affil=岡山大学教務FD 委員会

affil-num=5
en-affil=
kn-affil=岡山大学教務FD 委員会
en-keyword=Undernourished students
kn-keyword=Undernourished students
en-keyword=breakfast
kn-keyword=breakfast
en-keyword=lunchtime
kn-keyword=lunchtime
en-keyword=statistical significance
kn-keyword=statistical significance
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=105
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=20160201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=61
end-page=73
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2014.
kn-title=公表学術論文等リスト　2014
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=55
end-page=59
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of fasting and re-feeding on aquaporin gene expression in liver and kidney of the chicken
kn-title=ニワトリにおける肝臓と腎臓のアクアポリン（AQP）遺伝子発現に対する絶食と再給餌の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Aquaporin (AQP) which is a water channel, is also including the group (aquaglyceroporin) letting glycerol or urea go through as well as a water molecule. In mammals, AQP7 and AQP9 which are aquaglyceroporin have a role in the transportation of glycerol related to the gluconeogenesis in the liver. It is reported that AQP9 increases uptake of glycerol in the liver, when blood glucose level decreases. This study investigated the effect of AQP9 and AQP7
mRNA levels in liver and kidney in relation to the fasting and the re-feeding in the chicks. The purpose of this study was to determine whether the functions of AQP9 and AQP7 in the chicks are similar to mammals. One-day old chicks were kept with free access to water and food until 7-days
old. At 7-days old, chicks were divided into three groups of 6 chicks : a control groups with free access to food and water and a fasting group with free access to water but not to water for 24 hours, a re-feeding group with free access to
food and water for 6 hours after fasting treatment. At the end of the experiments all the chicks were sacrificed and blood and tissues (liver and kidney) were taken for measurement of blood glucose levels and mRNA levels. Total RNA extracted and reverse transcription was performed. AQP7 and AQP9 and the phosphoenolpyruvate carboxykinase (PEPCK) mRNA levels were measured by using real-time PCR. In the fasting group, body weight significantly decreased as compared with control group, but
blood glucose levels did not decrease. In the re-feeding groups, blood glucose levels significantly increased as compared with control group and fasting group. In the liver, the fasting and the re-feeding treatment did not affect AQP7 and AQP9 mRNA levels. In the kidney, the fasting did not affect AQP7 and AQP9 mRNA levels, but the re-feeding treatment significantly increased AQP7 and AQP9 mRNA levels. In the liver, PEKCK mRNA levels
significantly decreased in fasting group and further decreased in the re-feeding group. The re-feeding treatment significantly increased PEPCK mRNA levels in the kidney. From these results, kidney may play a roll in the increase of
blood glucose levels at the re-feeding. But, a roll in gluconeogenesis of liver is not clear in the chicks. Therefore, further study is necessary to understand the gluconeogenesis in the chicks.
en-copyright=
kn-copyright=

en-aut-name=SaitoNoboru
en-aut-sei=Saito
en-aut-mei=Noboru
kn-aut-name=齋藤昇
kn-aut-sei=齋藤
kn-aut-mei=昇
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=chicken
kn-keyword=chicken
en-keyword=aquaporin
kn-keyword=aquaporin
en-keyword=plasma glucose
kn-keyword=plasma glucose
en-keyword=fasting
kn-keyword=fasting
en-keyword=liver
kn-keyword=liver
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=49
end-page=53
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Development of a new computer-assisted technigue for efficient usage of latent heat with computer system
kn-title=施設栽培における潜熱利用に関するコンピュータ利用技術の開発
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　In protected horticulture, cooling techniques with latent heat has been effective for improving the growth of various kinds of plants in the summer season. It was necessary to understand special knowledge of the VETH diagrammatic
drawing for effective operation of cooling with latent heat. In order to obtain the information from VETH diagrammatic drawing, it was necessary to perform a lot of calculations. It was difficult to obtain such information simply. So, a device and software were developed for getting
effective information in order to manage cooling of latent heat. The developed device and software were run on the Ubiquitous Environment Control System (UECS). The rules for controlling the environment of UECS was were set up on
a web site, and an information communication technique was used for controlling the environment. The developed ventilation calculated node gathered the necessary information from the other nodes via LAN automatically. The node calculated valuable information for cooling with
latent heat, including the ventilation rate, and sent the calculated values, after entering the constant parameters using an http server, which was installed in the node. The developed software was run on a personal computer. The software could be used after describing various information
for calculating ventilation rate and evaporation rate at the setting file. It calculated these values, and users were shown the data using graphs and text files. As a result, we could get
effective information concerning cooling latent heat fairly easily by using of the developed node and the software.
en-copyright=
kn-copyright=

en-aut-name=YasubaKen-ichiro
en-aut-sei=Yasuba
en-aut-mei=Ken-ichiro
kn-aut-name=安場健一郎
kn-aut-sei=安場
kn-aut-mei=健一郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=latent heat
kn-keyword=latent heat
en-keyword=computer
kn-keyword=computer
en-keyword=fogging
kn-keyword=fogging
en-keyword=ubiquitous environment control system
kn-keyword=ubiquitous environment control system
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=35
end-page=47
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Microbiological Research on the Traditional Alcoholic Fermented Milk“Airag”in Mongolia
kn-title=モンゴルの伝統的アルコール発酵乳アイラグに関する微生物学的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Airag, a traditional fermented milk well loved by Mongolians, has been drunk habitually since
ancient times as a unique drink. It is produced from cow, mare and camel milk by a traditional method using indigenous starter cultures containing lactic acid bacteria, yeasts and other fermentative microorganisms. Spontaneously fermented milk products have for centuries been consumed for their therapeutic value in promoting health and well-being, especially among the pastoral communities in Mongolia. Most traditional fermentations are conducted as uncontrolled processes, however, increasing
research, such as that focused on compositional properties and microbial biota properties, offers
prospective views for improving spontaneous fermented products with respect to safety, shelf life, sensory characteristics, and nutritional and functional qualities. The aim of this article is to investigate progress in the microbiological research on the traditional alcoholic fermented milk, airag, in Mongolia and to give an outline of the traditional preparation processes.
en-copyright=
kn-copyright=

en-aut-name=MiyamotoTaku
en-aut-sei=Miyamoto
en-aut-mei=Taku
kn-aut-name=宮本拓
kn-aut-sei=宮本
kn-aut-mei=拓
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=airag
kn-keyword=airag
en-keyword=alcoholic fermented milk
kn-keyword=alcoholic fermented milk
en-keyword=lactic acid bacteria
kn-keyword=lactic acid bacteria
en-keyword=yeasts
kn-keyword=yeasts
en-keyword=traditional preparation process
kn-keyword=traditional preparation process
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=23
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Why do rabbits ingest their feces ?
kn-title=ウサギはなぜ糞を食べる？
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　The utilization of dietary energy and protein must depend on microbial activity in the gut in every herbivore. For animals adopting the cecum as a microbial habitat, from the viewpoint of the protein utilization, the position of the cecum in the digestive tract is less advantageous than that of foregut fermenters. As a solution to this problem, cecum fermenters perform cecotrophy in order to utilize microbial protein proliferating in the cecum. Cecotrophy is supported by the mechanism that separates microbes from digesta in the colon, sends them back into the cecum and promotes microbial proliferation in the cecum. The colonic separation mechanism can be classified into two types. One is the mucus-trap type separation of bacteria with mucus from digesta in the proximal colon of rodents.
The other is the wash-back type separation of particle phase of the colonic contents and liquid phase containing microbes in the colon of rabbits. For microbes guaranteed to inhabit the cecum under colonic separation mechanism, it is necessary for them to obtain sufficient nutrients for survival and proliferation. The source of nitrogen is easily obtained as urea transfered from the blood flow. On the other hand, the energy source is considered to be the limiting factor for bacterial proliferation due to
the limited amount of easily usable energy source. In fact, cecal microbial proliferation of rabbits and guinea pigs increases when adding indigestible but fermentable sugars to the feed. As a result, the amount of cecotrophy increases, and the percentage of nitrogen accumulation in the body tends to increase.
en-copyright=
kn-copyright=

en-aut-name=SakaguchiEi
en-aut-sei=Sakaguchi
en-aut-mei=Ei
kn-aut-name=坂口英
kn-aut-sei=坂口
kn-aut-mei=英
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
en-keyword=Small herbivore
kn-keyword=Small herbivore
en-keyword=Cecum
kn-keyword=Cecum
en-keyword=Nitrogen metabolism
kn-keyword=Nitrogen metabolism
en-keyword=Cecotrophy
kn-keyword=Cecotrophy
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=13
end-page=21
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=水稲品種の乾物生産，収量と窒素利用効率に及ぼす肥料と施肥法の影響
kn-title=Effects of different types of fertilizers and methods on dry matter production, yield and nitrogen use efficiency of rice cultivars under field conditions
en-subtitle=
kn-subtitle=
en-abstract=　水稲の乾物生産，収量，窒素利用効率に及ぼす肥料と施肥法の影響を試験するため，水稲品種日本晴とタカナリを供試し，対照区（無施肥），慣行施肥区（分割追肥），深層追肥区，標準緩効性肥料基肥施肥区，倍量緩効性肥料基肥施肥区の５試験区を用いて栽培を行った．窒素施肥とともに，乾物生産が増大し，最終乾物重はタカナリでは深層追肥区，日本晴では標準緩効性肥料基肥施肥区で最も高くなった．乾物重の相違には個体群成長速度と葉面積指数が主として影響していた．収穫期の乾物重が大きいほど，窒素蓄積が多くなった．窒素蓄積量は両品種ともに深層追肥区で多くなった．施肥量の増加は穂数と㎡当たり穎花数を増加させた．タカナリの収量が高いことには，シンク容量が大きいことが関係した．両品種ともに慣行施肥区に比べ深層追肥区の収量が高くなったが，これにはシンク容量の拡大が関係していた．深層追肥区，標準緩効性肥料基肥施肥区ともに慣行施肥区に比べ窒素回収効率，部分要因生産性ともに向上したが，省力・低コストの観点からは施肥効率の高い緩効性肥料の利用が推奨された．
kn-abstract=　To examine the effects of different types of fertilizers and application methods on dry matter production, yield, nitrogen accumulation and use efficiency in rice cultivars, we used two rice cultivars (Nipponbare and Takanari) and five fertilizer methods, i.e. Control (0N), Conventional method, Deep fertilizer
method, Standard fertilizer method and High fertilizer method in 2009. Dry matter production
was more markedly increased with nitrogen fertilizer application than in control, and it was higher with deep fertilizer application in Takanari and standard fertilizer application in Nipponbare, respectively. The differences in dry matter production resulted from CGR and mean LAI in rice cultivars. Greater dry matter production was accompanied with the nitrogen accumulation at harvesting. Rice cultivars accumulated the largest amount of nitrogen at deep fertilizer application. Higher fertilizer application increased the number of panicle and total spikelets m–2. The higher grain yield in Takanari
resulted from the larger sink capacity. The grain yield of rice cultivars tended to be higher with deep fertilizer application due to the increase in sink capacity. Both deep fertilizer application and basal application of slow-release fertilizer increased the recovery efficiency and partial factor productivity of applied N, however, using slow-release fertilizer is recommended in terms of labor saving and lower
cost.
en-copyright=
kn-copyright=

en-aut-name=YabeRyo
en-aut-sei=Yabe
en-aut-mei=Ryo
kn-aut-name=矢部亮
kn-aut-sei=矢部
kn-aut-mei=亮
aut-affil-num=1
ORCID=

en-aut-name=Nguyen Quang Co
en-aut-sei=Nguyen Quang Co
en-aut-mei=
kn-aut-name=グエン クアン コ
kn-aut-sei=グエン クアン コ
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Trinh Thi Sen
en-aut-sei=Trinh Thi Sen
en-aut-mei=
kn-aut-name=トリン ティ セン
kn-aut-sei=トリン ティ セン
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学自然科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学環境生命科学研究科

affil-num=3
en-affil=
kn-affil=岡山大学自然科学研究科

affil-num=4
en-affil=
kn-affil=岡山大学環境生命科学研究科
en-keyword=Conventional method
kn-keyword=Conventional method
en-keyword=deep fertilizer method
kn-keyword=deep fertilizer method
en-keyword=nitrogen use efficiency
kn-keyword=nitrogen use efficiency
en-keyword=rice (Oryza sativa L.)
kn-keyword=rice (Oryza sativa L.)
en-keyword=slow-release fertilizer
kn-keyword=slow-release fertilizer
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=5
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=好酸性鉄酸化細菌 Acidithiobacillus ferrooxidans の染色体凝集・分配タンパク質（ScpB）の性質
kn-title=Characterization of a putative chromosome segregation and condensation protein (ScpB) in an acidophilic iron‒oxidizing bacterium Acidithiobacillus ferrooxidans
en-subtitle=
kn-subtitle=
en-abstract=　Acidithiobacillus ferrooxidans は，低品位の銅鉱石から銅を回収するバイオリーチングにおいて使用される微生物
の一つである．この細菌は，エネルギー源として二価鉄イオンや還元型無機硫黄化合物（RISC）を使用する．鉄の酸化に関与する遺伝子の転写は，A. ferrooxidans が RISC で生育したときには抑制されるが，RISC の酸化に関与すると考えられている遺伝子の転写は活性化されることが知られている．硫黄やテトラチオン酸で生育したときにその発現が上方制御される硫化水素：キノン酸化還元酵素のすぐ上流に，ヘリックスターンへリックスモティーフを持つ，ScpB と推定されるタンパク質をコードする遺伝子が存在していた．鉄，硫黄，テトラチオン酸生育細胞から調製した cDNA を用いた半定量的 PCR 分析の結果，硫黄やテトラチオン酸で生育した細胞内の scpB 遺伝子の転写は，鉄生育細胞と比較すると上方制御されていた．組換え ScpB タンパク質を用いたゲルシフトアッセイ法で，ScpB
が転写制御因子として機能するかどうかを調べた．その結果，ScpB は DNA に結合したが，結合の特異性はなかった．ScpB が直接 DNA と相互作用する報告はこれまでになかった．
kn-abstract=　Acidithiobacillus ferrooxidans is one of the most widely used microorganisms in bioleaching operations to recover copper from low-grade copper sulfide. This bacterium uses ferrous iron and reduced inorganic sulfur compounds (RISCs) as energy sources. Transcriptions of genes thought to be involved in the oxidation of RISCs have been known to be highly activated in A. ferrooxidans cells grown on RISCs, while transcriptions of genes involved in the iron oxidation were repressed in the cells grown on
RISCs. A gene encoding a putative chromosome segregation and condensation protein (ScpB) with a
helix-turn-helix motif was found in the upstream region of sulfide : quinone oxidoreductase gene, whose expression was up-regulated in cells grown in sulfur and tetrathionate. A semi-quantitative PCR analysis using cDNA prepared from iron-, sulfur-, or tetrathionate-grown cells revealed that the transcription of scpB was up-regulated in cells grown on sulfur or tetrathionate as the energy source. Electrophoretic mobility shift assays were employed to examine whether the ScpB functions as a transcription factor. The result indicated that the recombinant His-tagged ScpB protein was able to nonspecifically bind in
vitro to DNA. This is the first report on a direct association of ScpB with DNA.
en-copyright=
kn-copyright=

en-aut-name=KamimuraKazuo
en-aut-sei=Kamimura
en-aut-mei=Kazuo
kn-aut-name=上村一雄
kn-aut-sei=上村
kn-aut-mei=一雄
aut-affil-num=1
ORCID=

en-aut-name=NagataNozomu
en-aut-sei=Nagata
en-aut-mei=Nozomu
kn-aut-name=長田臨
kn-aut-sei=長田
kn-aut-mei=臨
aut-affil-num=2
ORCID=

en-aut-name=KikumotoMei
en-aut-sei=Kikumoto
en-aut-mei=Mei
kn-aut-name=菊本愛生
kn-aut-sei=菊本
kn-aut-mei=愛生
aut-affil-num=3
ORCID=

en-aut-name=SharminSultana
en-aut-sei=Sharmin
en-aut-mei=Sultana
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=WakaiSatoshi
en-aut-sei=Wakai
en-aut-mei=Satoshi
kn-aut-name=若井暁
kn-aut-sei=若井
kn-aut-mei=暁
aut-affil-num=5
ORCID=

en-aut-name=KanaoTadayoshi
en-aut-sei=Kanao
en-aut-mei=Tadayoshi
kn-aut-name=金尾忠芳
kn-aut-sei=金尾
kn-aut-mei=忠芳
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=3
en-affil=
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=4
en-affil=
kn-affil=岡山大学大学院環境生命科学研究科

affil-num=5
en-affil=
kn-affil=神戸大学自然科学系先端融合研究環

affil-num=6
en-affil=
kn-affil=神戸大学自然科学系先端融合研究環
en-keyword=Acidithiobacillus ferrooxidans
kn-keyword=Acidithiobacillus ferrooxidans
en-keyword=Acidophile
kn-keyword=Acidophile
en-keyword=ScpB
kn-keyword=ScpB
en-keyword=transcription factor
kn-keyword=transcription factor
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=1
end-page=4
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Improvement of dye-mediated dehydrogenase activity of pyranose oxidase by site-directed mutagenesis
kn-title=部位特異的変異によるピラノース酸化酵素の色素依存性脱水素酵素活性の向上
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Pyranose oxidase (EC 1.1.3.10 ; PROD) catalyzes the oxidation of aldopyranoses at the position C‒2
to yield the corresponding 2‒keto-aldoses and H2O2 , using oxygen as an electron acceptor. The enzyme shows broad substrate specificity as well as reactivity for 1,5‒anhydro‒d‒glucitol (1,5‒AG), which is known as a clinical glycemic marker. It is considered that the reactivity of PROD for 1,5‒AG is useful in the development of an amperometric-type biosensor, which is a convenient diagnostic device for selfmonitoring
blood glucose (SMBG). However, the levels of dissolved oxygen in blood affect biosensor
systems that are equipped with an artificial electron mediator. In the present study, we attempted to develop an O2‒insensitive oxidase that would improve the dye-mediated dehydrogenase activity. We performed site-directed mutagenesis on PROD isolated from basidiomycetous fungus No. 52, which generated 11 mutants. The amino acid substitution Q421A exhibited a significant decrease (8.8% of wild type) in its oxidase activity, whereas it maintained its dehydrogenase activity (67% of wild type). In this study, we characterized PROD mutants from basidiomycetous fungus No. 52, which showed improved dye-mediated dehydrogenase activity.
en-copyright=
kn-copyright=

en-aut-name=ArakiToshio
en-aut-sei=Araki
en-aut-mei=Toshio
kn-aut-name=荒木俊雄
kn-aut-sei=荒木
kn-aut-mei=俊雄
aut-affil-num=1
ORCID=

en-aut-name=NakatsukaTomoko
en-aut-sei=Nakatsuka
en-aut-mei=Tomoko
kn-aut-name=中柄朋子
kn-aut-sei=中柄
kn-aut-mei=朋子
aut-affil-num=2
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=3
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=池田食研株式会社

affil-num=2
en-affil=
kn-affil=池田食研株式会社

affil-num=3
en-affil=
kn-affil=岡山大学農学部

affil-num=4
en-affil=
kn-affil=岡山大学農学部
en-keyword=pyranose oxidase
kn-keyword=pyranose oxidase
en-keyword=1,5-anhydro-d-glucitol
kn-keyword=1,5-anhydro-d-glucitol
en-keyword=biosensor
kn-keyword=biosensor
en-keyword=site-directed mutagenesis
kn-keyword=site-directed mutagenesis
en-keyword=SMBG
kn-keyword=SMBG
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=104
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=20150201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=45
end-page=59
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2013.
kn-title=公表学術論文等リスト　2013
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=37
end-page=43
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Research on capsiconinoid contents, nonpungent capsaicinoid analogues, in Capsicum cultivars
kn-title=トウガラシにおける新規カプサイシン類似物質・カプシコニノイドの含量
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　In the course of analyses of nonpungent capsaicinoid
analogs named capsinoids, two unknown compounds were
discovered in pepper fruits. These compounds were isolated
from the fruit of ‘CCB’(Capsicum baccatum var.
praetermissum). Their structures were determined to be
coniferyl (E)‒8‒methyl‒6‒nonenoate and coniferyl
8‒methylnonanoate. These novel capsaicin analogs were
named capsiconiate and dihydrocapsiconiate, respectively,
and the coniferyl ester group was named capsiconinoid.
Capsiconinoids have agonist activity for transient receptor
potential vanilloid type 1, and their pungency is very low, as
similar to that of capsinoids. Cultivars containing high
levels of capsiconinoid are considered to be important for
vegetable or dietary supplement. HPLC analysis was
conducted to determine capsiconinoid content in fruits of
54 Capsicum cultivars : 28 cultivars of C. annuum, 9 of
C. baccatum, 12 of C. chinense, 4 of C. frutescens, and 1 of
C. pubescens. Twelve cultivars contained capsiconinoids.
‘CCB’ showed the highest capsiconinoid content level (3314
μg・g‒1 DW) and ‘Charapita’ (C. chinense) had the second
highest (2694 μg・g‒1 DW). The other 10 cultivars contained
much lower capsiconinoid than these two cultivars
(<300 μg・g‒1 DW). Time-course analysis during fruit
development clarified that capsiconinoid content increased
and reached maximum level in green mature fruit.
Capsaicinoid contents also increased, correspondingly. As
fruits matured, capsiconinoid contents decreased rapidly,
while capsaicinoid content either did not change or
decreased slightly.
en-copyright=
kn-copyright=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=田中義行
kn-aut-sei=田中
kn-aut-mei=義行
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=pepper
kn-keyword=pepper
en-keyword=capsaicinoid analogs
kn-keyword=capsaicinoid analogs
en-keyword=capsiconinoid
kn-keyword=capsiconinoid
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=31
end-page=36
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Isolation and characterization of plant immune-priming chemicals
kn-title=植物免疫プライミング剤の単離と作用機序解明
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Plant disease resistance inducers, so-called plant
activators, are agrochemicals that protect crops from
pathogens. They confer long-lasting resistance against a
broad range of diseases by activating their immune system.
Since plant activators impinge on host plants, unlike
commonly-used pesticides which directly target pathogens,
no drug-resistant microbes for plant activators have been
found so far in the field. They originated from probenazole
(Oryzemate ) and have been widely used over 30 years for
the protection of paddy-field rice from blast fungus and
bacterial leaf blight in East Asia. In spite of the advantages
of plant activators, their application is still limited. The lack
of both knowledge about their modes of action and an
appropriate high-throughput screening system restrict the
isolation of novel compounds. We established a highthroughput
chemical screening procedure to identify plant
immune-priming compounds which increase but do not
directly induce immune responses in Arabidopsis
suspension cells upon infection of the bacterial pathogen,
Pseudomonas syringae pv. tomato DC3000 avrRpm1. From
the screening of a commercially available chemical library
of 10,000 diverse small organic molecules, we identified
seven compounds that prime the immune response and we
designated them ‘imprimatins’ for immune-priming
chemicals. The isolated compound, imprimatin C1 activates
the expression of defense-related genes independent of
pathogen and functions as a weak analog of salicylic acid
(SA). Those originally-isolated three compounds and their
four derivatives were classified into two groups with distinct
molecular structures and they weve named imprimatins A
and B. We found that they conferred disease resistance in
plants by inhibiting both a known and a previously
unknown SA glucosyltransferase (SAGT). Imprimatins and
their targets are useful for the development of practical
plant activators and also their modes of action might give a
clue for novel crop protection technology.
en-copyright=
kn-copyright=

en-aut-name=NoutoshiYoshiteru
en-aut-sei=Noutoshi
en-aut-mei=Yoshiteru
kn-aut-name=能年義輝
kn-aut-sei=能年
kn-aut-mei=義輝
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=plant disease resistance
kn-keyword=plant disease resistance
en-keyword=plant immunity
kn-keyword=plant immunity
en-keyword=disease resistance inducer
kn-keyword=disease resistance inducer
en-keyword=plant activator
kn-keyword=plant activator
en-keyword=chemical biology
kn-keyword=chemical biology
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=21
end-page=30
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=コムギ染色体欠損系統を用いた新規活性型レトロトランスポゾン TriRe-1 の分子遺伝学的解析
kn-title=Characterization of a novel retrotransposon TriRe‒1 using nullisomic-tetrasomic lines of hexaploid wheat
en-subtitle=
kn-subtitle=
en-abstract=　レトロトランスポゾンは植物ゲノムの主要な構成要素であり，コムギゲノムにおいてはその80ｵを占める．特に
LTR 型レトロトランスポゾンの割合が高く，ゲノムの拡大，配列の多様性およびゲノム構造変異等に大きく寄与し
てきたと考えられている．これら配列は自身のコピー配列を複製し増幅するため，ゲノム中には数百，数千に及ぶコ
ピー配列をもつ．また，ゲノム進化の過程において多数のファミリーを形成してきた．これら多数のファミリーのう
ち，現在でも転移活性を示す活性型ファミリーは，品種間において高い挿入多型を示すことが知られている．このよ
うな挿入多型は，連鎖解析および系統解析等各種遺伝解析に利用可能である. 本研究では，コムギにおける新規活性
型レトロトランスポゾンファミリー TriRe-1 の特徴を詳細に解析した．TriRe-1 は転移に必要なタンパク質をコー
ドする内部配列をもち，また日本で育成されたコムギ近縁品種間においても高い挿入多型を示したため，現在でも転
移活性を有している，もしくはごく最近まで転移していた可能性が高いと考えられた．一方で，コムギ染色体欠損系
統（ナリソミックテトラソミック系統）を用い，TriRe-1 の挿入箇所を比較解析した．その結果，大部分の挿入箇所
は複数の同祖染色体に存在すると考えられたが，Ｂゲノムにおいて最も多くの特異的な挿入箇所が同定された．よっ
て，Ｂゲノム祖先種において活発に増幅してきた可能性が示唆された．今回の結果により，新規活性型レトロトラン
スポゾン TriRe-1 の品種間挿入多型を利用した DNA マーカー，また，各ゲノム（Ａ，Ｂ，Ｄゲノム）特異的な挿
入箇所を利用したゲノム識別性に優れた DNA マーカーの開発の可能性が期待される．
kn-abstract=　Retrotransposons constitute the large fraction (～80%) of the wheat genome where numerous and
diverse retrotransposon families exist, where especially the long terminal repeat (LTR) retrotransposon
family is known to be predominant. Thus, they have been considered to contribute to the genome
expansion, sequence diversification and the genome structure alternation in the wheat genome. In addition,
the insertion polymorphism of the LTR retrotransposon family among the cultivars has been
known to be quite useful for the genetic analysis such as the linkage mapping and the phylogenetic studies.
Here, we report the characteristics of a novel active LTR retrotransposon family TriRe‒1, which
belongs to the Ty1‒copia group in the hexaploid wheat (Triticum aestivum L.) genome. This retroelement
appears to encode all proteins required for the transposition and showed high insertion polymorphism
among the hexaploid wheat cultivars, suggesting its potential of transpositional activity with at least
recent transposition during wheat evolution. We studied the chromosomal localization of the TriRe‒1
insertion site based on the genome-wide comparative analysis using the nullisomic-tetrasomic lines of
the cultivar Chinese Spring. The results showed that although the majority of the TriRe‒1 insertion sites
exist across the homoeologous chromosomes of A, B or D genomes, a higher number of insertions in the
B genome was detected compared to A or D genome, suggesting a specific amplification in the history
of B genome progenitors. In conclusion, a novel LTR retrotransposon TriRe‒1 should be valuable for the
development of molecular markers based on insertion polymorphism among the cultivars, and also the
genome-specific TriRe‒1 insertion site can be utilized to study evolutional history of wheat genomes.
en-copyright=
kn-copyright=

en-aut-name=MondenYuki
en-aut-sei=Monden
en-aut-mei=Yuki
kn-aut-name=門田有希
kn-aut-sei=門田
kn-aut-mei=有希
aut-affil-num=1
ORCID=

en-aut-name=TakaiTakeru
en-aut-sei=Takai
en-aut-mei=Takeru
kn-aut-name=高井健
kn-aut-sei=高井
kn-aut-mei=健
aut-affil-num=2
ORCID=

en-aut-name=TaharaMakoto
en-aut-sei=Tahara
en-aut-mei=Makoto
kn-aut-name=田原誠
kn-aut-sei=田原
kn-aut-mei=誠
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=Retrotransposon
kn-keyword=Retrotransposon
en-keyword=Wheat
kn-keyword=Wheat
en-keyword=Molecular markers
kn-keyword=Molecular markers
en-keyword=Nullisomic-tetrasomic lines
kn-keyword=Nullisomic-tetrasomic lines
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=11
end-page=19
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of potassium nutrition on fruit development and yield of substrate grown strawberry
kn-title=養液栽培イチゴの生育・収量と果実発育に及ぼすカリウム栄養の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　Effect of K nutrition on sugar and organic acid concentration in fruit and yield of strawberry
cultivars (Fragaria ×ananassa Duch.; Nyoho, Toyonoka, Sachinoka, Asukarubi, Akihime, Tochiotome,
and Sagahonoka) was investigated. Nutrient solution (NO3 8, NH4 1, P 1, K 4, Ca 2, Mg 1, SO4 1 ; mM)
was modified to contain 0 to 4mM of K by replacing K with Ca and supplied from the beginning of
flowering. K in the drainage decreased to a trace level 3 weeks after the beginning of treatment except
for 4mM‒K solution. Absorption of NO3 apparently decreased in plants supplied 0mM‒K solution, but
little difference was observed among the other 3 solutions. K concentration in petiole decreased linearly
with decrease in K concentration in solutions, but there was little difference between the concentrations
of leaflet of plants supplied with 4 and 2mM‒K solutions. No difference was observed in concentrations
of sugars and organic acids in fruit in primary inflorescence. In the second inflorescence, organic acids
and K concentration in fruit linearly decreased with decrease in K in supplied solutions while sugar
concentration of fruit decreased significantly only in plants supplied 0mM‒K solution. When 0.5 to
4mM‒K solutions were supplied from 2 weeks after planting, marketable fruit yield was smallest in
plants supplied 0.5mM‒K solution followed, by that supplied 1mM‒K solution, and largest in that
supplied with 2mM‒K solution. Almost all K supplied with 2mM‒K solution was absorbed by
strawberry plants and no difference was observed in quality and K concentration of fruit, yield and plant
growth between the plants supplied with 2 and 4mM‒K solutions. It may be suitable to reduce the
concentration of K in nutrient solution by half for substrate production of strawberry.
en-copyright=
kn-copyright=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=1
ORCID=

en-aut-name=OhmoriToshimasa
en-aut-sei=Ohmori
en-aut-mei=Toshimasa
kn-aut-name=大森敏正
kn-aut-sei=大森
kn-aut-mei=敏正
aut-affil-num=2
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=3
ORCID=

en-aut-name=TanakaYoshiyuki
en-aut-sei=Tanaka
en-aut-mei=Yoshiyuki
kn-aut-name=田中義行
kn-aut-sei=田中
kn-aut-mei=義行
aut-affil-num=4
ORCID=

en-aut-name=MurakamiKenji
en-aut-sei=Murakami
en-aut-mei=Kenji
kn-aut-name=村上賢治
kn-aut-sei=村上
kn-aut-mei=賢治
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=農林水産省中国四国農政局

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=石川県立大学生物資源環境学部
en-keyword=Fragaria ×ananassa Duch.
kn-keyword=Fragaria ×ananassa Duch.
en-keyword=K/Ca ratio
kn-keyword=K/Ca ratio
en-keyword=organic acids
kn-keyword=organic acids
en-keyword=sugars
kn-keyword=sugars
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=5
end-page=9
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Characterization of L-Arginine Oxidase Made from L-Glutamate Oxidase
kn-title=高基質特異性L-グルタミン酸オキシダーゼより作成した基質特異性改変酵素L-アルギニンオキシダーゼの性質検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=　L‒Glutamate oxidase (LGOX) from Streptomyces sp. X‒119‒6 has strict substrate specificity toward
L‒glutamate. Recently, we solved the X‒ray crystal structure of LGOX and this revealed that Arg305 in
the active site is the key residue involved in substrate recognition. Therefore, we created 19 mutant
enzymes of R305X‒LGOX by saturation mutagenesis. One of them R305D‒LGOX, Arg305 substituted
with Asp exhibited oxidase activity for L‒Arg. Optimum pH of R305D‒LGOX mutant enzyme was pH
8.5. Interestingly, the activity of R305D‒LGOX toward L‒Arg was inhibited by phosphate. And furthermore,
the substrate specificity of R305D‒LGOX was affected by using buffer. The results of inhibition
analysis suggest, that phosphate is a competitive inhibitor of R305D‒LGOX when L‒Arg is used as
substrate. Kinetic analysis of R305D‒LGOX showed that Km value and kcat value of R305D‒LGOX toward
l-Arg were 0.68 mM and 6.7 s-1 respectively. In this study, we showed that R305D‒LGOX mutant
enzyme is a novel l-arginine oxidase and useful for l-arginine biosensor.
en-copyright=
kn-copyright=

en-aut-name=NakaiRyuichiro
en-aut-sei=Nakai
en-aut-mei=Ryuichiro
kn-aut-name=中井隆一郎
kn-aut-sei=中井
kn-aut-mei=隆一郎
aut-affil-num=1
ORCID=

en-aut-name=FujinoShihoko
en-aut-sei=Fujino
en-aut-mei=Shihoko
kn-aut-name=藤野志保子
kn-aut-sei=藤野
kn-aut-mei=志保子
aut-affil-num=2
ORCID=

en-aut-name=UtsumiTomohiro
en-aut-sei=Utsumi
en-aut-mei=Tomohiro
kn-aut-name=内海友宏
kn-aut-sei=内海
kn-aut-mei=友宏
aut-affil-num=3
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=4
ORCID=

en-aut-name=KusakabeaHitoshi
en-aut-sei=Kusakabea
en-aut-mei=Hitoshi
kn-aut-name=日下部均
kn-aut-sei=日下部
kn-aut-mei=均
aut-affil-num=5
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=㈱エンザイムセンサ

affil-num=6
en-affil=
kn-affil=岡山大学
en-keyword=L-glutamate oxidase
kn-keyword=L-glutamate oxidase
en-keyword=L-arginine oxidase
kn-keyword=L-arginine oxidase
en-keyword=biosensor
kn-keyword=biosensor
en-keyword=modified substrate specificity
kn-keyword=modified substrate specificity
en-keyword=L-amino acid oxidase
kn-keyword=L-amino acid oxidase
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=1
end-page=4
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=大豆発芽時期におけるグリシニン分解酵素の活性変動
kn-title=Changes in Glycinin‒Digesting Protease Activity During Soybean Germination.
en-subtitle=
kn-subtitle=
en-abstract=　大豆発芽期におけるグリシニン分解酵素 (98 kDa SBP) の活性変動を解析した．大豆種子を４時間水で膨潤後， 25
℃ 暗黒下で発芽させた．経時的にサンプリングを行い，2M NaCl を含むトリス緩衝液 (㏗ 7.0) により粗酵素を抽出
後，グリシニン由来のトリプシン分解ペプチドを基質としてグリシニン分解酵素の活性変動を逆相 HPLC により追
跡した．その結果，種子膨潤後４日間比活性はほぼ一定の値を保ち，以後徐々に低下することが分かった．次いで，
粗酵素溶液からイオン交換 HPLC により98 kDa SBP を部分精製するとともに，発芽期における 98 kDa SBP の消長
を解析したところ，98 kDa SBP は乾燥種子及び各発芽段階の種子中全てに認められ，かつグリシン分解活性もグリ
シニン由来のトリプシン分解ペプチド基質に対する活性と同様に認められた．以上の結果から，98 kDa SBP は種子
発芽に伴い誘導されるプロテアーゼではなく，種子貯蔵型のプロテアーゼであることが明らかになった．
kn-abstract=　Changes in glycinin-digesting protease activity during soybean germination have been investigated.
The glycinin-digesting protease activities of imbibed or germinated soybean seed were assayed by
RP‒HPLC using a tryptic peptide from CM‒glycinin or by SDS‒PAGE using CM‒glycinin as the endogenous
substrate. Proteolytic activities of the germinated soybean seeds were found through the whole
period of germination, the activities were maintained significantly unchanged during germination for 4
days, and then those specific activities declined slowly. AE‒HPLC analysis of the glycinin-digesting
protease in the imbibed or germinated soybean seeds showed unchanged peaks corresponding to glycinin-
digesting activity, suggesting that the glycinin-digesting protease was not induced during germination
but had already been synthesized during seed maturation.
en-copyright=
kn-copyright=

en-aut-name=Md. Akhtaruzzaman
en-aut-sei=Md. Akhtaruzzaman
en-aut-mei=
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MaedaMegumi
en-aut-sei=Maeda
en-aut-mei=Megumi
kn-aut-name=前田恵
kn-aut-sei=前田
kn-aut-mei=恵
aut-affil-num=2
ORCID=

en-aut-name=KitagawaKeiko
en-aut-sei=Kitagawa
en-aut-mei=Keiko
kn-aut-name=北川恵子
kn-aut-sei=北川
kn-aut-mei=恵子
aut-affil-num=3
ORCID=

en-aut-name=TakagiShigeaki
en-aut-sei=Takagi
en-aut-mei=Shigeaki
kn-aut-name=高木茂明
kn-aut-sei=高木
kn-aut-mei=茂明
aut-affil-num=4
ORCID=

en-aut-name=KimuraYoshinobu
en-aut-sei=Kimura
en-aut-mei=Yoshinobu
kn-aut-name=木村吉伸
kn-aut-sei=木村
kn-aut-mei=吉伸
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岡山大学
en-keyword=Plant protease
kn-keyword=Plant protease
en-keyword=glycinin
kn-keyword=glycinin
en-keyword=germination
kn-keyword=germination
en-keyword=Glycine max
kn-keyword=Glycine max
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=103
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=69
end-page=83
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2012.
kn-title=公表学術論文等リスト　2012
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=63
end-page=68
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Plasmodium Falciparum-infected Erythrocytes Adhere to Class A Scavenger Receptor, SR-A
kn-title=マラリア重症化関連新規宿主因子としてのScavenger receptor Aの同定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Severe falciparum malaria such as cerebral malaria and severe anemia is leading causes of morbidity and mortality. Plasmodium falciparum-infected red blood cells (pRBC) adhere to the endothelial cells via receptors expressed on the surface of the endothelial cells, and sequester in the microvasculature of several organs. Severe anemia, which may be due to a number of factors including rupture of the pRBC and phagocytosis of pRBC, is another cause of death. However, the molecular mechanism underlying both the cytoadherence and erythrophagocytosis related with severe malaria is not completely understood. Here, we report that the pRBC bind to the class A scavenger receptor, scavenger receptor A (SR-A), which is expressed on the surface of the activated phagocytes.
　First, we confirmed mRNA expression of scavenger receptors in the various tissues of P. berghei ANKA-infected mice. The expression of SR-A mRNA in all tissues was enhanced for 7 days postinfection. We also confirmed mRNA expression of SR-A in the human macrophage cell line, THP-1 cells, cultivated with pRBC. SR-A mRNA expression in THP-1 cells with pRBCs was observed after 24 hr cultivation, but not RBCs. Then, to identify cytoadherence of pRBCs to SR-A, human SR-A cDNA was transfected to CHO cells (CHO-SR-A cells). pRBC adhered to the CHO-SR-A cells, but not to the CHO-mock cells. Interestingly, the cytoadherence of both mature stage and ring form pRBCs to the CHO-SR-A cells was observed. Anti-SR-A antibody, but not Anexin V, efficiently blocked the cytoadherence of the pRBC to the CHO-SR-A cells.
　These results may suggest that SR-A acts as a host factor related with cytoadherence of the pRBC, which contributes to our present understanding of the pathology of severe falciparum malaria.
en-copyright=
kn-copyright=

en-aut-name=HatabuToshimitsu
en-aut-sei=Hatabu
en-aut-mei=Toshimitsu
kn-aut-name=畑生俊光
kn-aut-sei=畑生
kn-aut-mei=俊光
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=severe malaria
kn-keyword=severe malaria
en-keyword=scavenger receptor
kn-keyword=scavenger receptor
en-keyword=cytoadherence
kn-keyword=cytoadherence
en-keyword=host-parasite relationship
kn-keyword=host-parasite relationship
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=53
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Development of New Irrigation System based on Water Relations Research for Citrus Fruit Production
kn-title=カンキツの水分生理特性の解析に 基づく「マルチ・ドリップ栽培技術」の開発
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A new cultivation system using drip irrigation and liquid fertilization methods combined with year-round plastic mulching system (DLYM system) was developed, expecting high quality and stable fruit production based on the research results on water relations of Satsuma mandarin (Citrus unshiu M.), where sugar accumulation in fruit was caused by an increase in translocation of photosynthates into fruit under drought stress.
　Water and/or nutrient solution is automatically supplied through the drip tubes laid under mulching sheets to give adequate water stress. Fruit quality was well controlled through the DLYM system. Sugar content of fruit increased by 2 Brix %, and functional components such as β‒cryptoxantin and β‒caroten increased by about 50% compared to juice from fruit grown on control trees. In addition, new technologies were developed, such as hydraulic design support system and water stress indicator sheet.
en-copyright=
kn-copyright=

en-aut-name=MorinagaKunihisa
en-aut-sei=Morinaga
en-aut-mei=Kunihisa
kn-aut-name=森永邦久
kn-aut-sei=森永
kn-aut-mei=邦久
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=water stress
kn-keyword=water stress
en-keyword=drip irrigation
kn-keyword=drip irrigation
en-keyword=mulching
kn-keyword=mulching
en-keyword=satuma mandarin
kn-keyword=satuma mandarin
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=43
end-page=51
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Sex-steroid Hormones on Lymphocyte Genesis in the Central Lymphoid Organs of Chickens
kn-title=ニワトリのリンパ球造成と性ステロイドホルモン
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Lymphocytes play essential roles as a kind of leukocyte in the defense mechanism of animals against infectious factors. Lymphocytes are classified into two subsets, T lymphocytes and B lymphocytes in mammals or avian species. In avian species, T lymphocytes differentiate and proliferate in the thymus which is a central lymphoid organ common to mammalians and avian species, whereas on the other hand, B lymphocytes have been known to occur in the bursa of Fabricius (bursa) which is a unique central lymphoid organ of birds. Steroid hormones, such as androgen and estrogen, have been reported to change differentiations and proliferations of these lymphocytes in corresponding lymphoid organs, indicating steroid hormones give influence lymphocyte development positively or negatively in the bursa and thymus of birds. Studying the relation between steroid hormones and lymphocyte development in the central lymphoid organs is important, because changes in the lymphocyte genesis in central organs of birds may result in altered levels of antibody production and immune functions related to T lymphocyte activity. We have studied effects of androgen and estrogen on lymphocyte differentiation and proliferation in the central lymphoid organs of chicken at Okayama University since the 1980s. In the present report, the results of these studies are summarized.
en-copyright=
kn-copyright=

en-aut-name=KondoYasuhiro
en-aut-sei=Kondo
en-aut-mei=Yasuhiro
kn-aut-name=近藤康博
kn-aut-sei=近藤
kn-aut-mei=康博
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=lymphocyte
kn-keyword=lymphocyte
en-keyword=steroid hormone
kn-keyword=steroid hormone
en-keyword=bursa of Fabricius
kn-keyword=bursa of Fabricius
en-keyword=thymus
kn-keyword=thymus
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=35
end-page=41
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Lighting Using Yellow LEDs Designed for Moth Control on Flowering Response of Chrysanthemum
kn-title=防蛾用黄色LED光がキクの開花反応に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=キクの防蛾と開花抑制に両用できる単一の光源を開発するために，青（ピーク波長：463nm），緑（519nm），黄緑（576nm），黄（597nm）および赤色（646nm）LED光が開花および切り花形質に及ぼす影響を調査した．青色光を除いて，放射照度が大きいほど発蕾までの日数が大きくなった．黄緑および黄色光は，赤色光とほぼ同等の開花抑制作用を有していた．いずれの光質および放射照度に関わらず，やなぎ葉数や花弁の展開異常の発生に差は見られなかった．次に，黄色LED光による暗期中断と終夜照明による影響を調査した．暗期中断と終夜照明では，やなぎ葉数を除く切り花形質に有意な差が見られたが，実用上の問題はなかった．開花抑制に必要となる放射照度の下限値は，終夜照明では約80mW m-2であり，暗期中断のほぼ半分であった．以上のことから，黄色LED光は，単一の光源としてキクの防蛾と開花抑制に両用することが可能であった．
kn-abstract=In order to develop a single light source which can be used both for moth control and flower inhibition in chrysanthemum, effects of blue (463nm), green (519nm), yellow green (576nm), yellow (597nm) and red light (646nm) LEDs on the flowering and the cut flower characteristics of chrysanthemum were investigated. As irradiance increased, the days to flower budding increased except under blue light. Yellow green and yellow LED had flower inhibiting effect equivalent to red LED. There was no difference in the crown bud number and the occurrence of abnormal flower irrespective of the light quality and irradiance. Next the, effects of night break and continuous lighting treatment by yellow LED on the flowering and cut flower characteristics of the chrysanthemum were investigated. There were significant differences in the cut flower characteristics except for the blade number on the neck in these treatments ; there was no practical problem with night break or continuous lighting. The minimum irradiance strength enough for flower inhibition in the continuous lighting treatment was about 80 mW m−2 that was half in night break treatment. Therefore, it is considered that yellow LED can be used as single light source for both moth control and flower inhibition in chrysanthemum.
en-copyright=
kn-copyright=

en-aut-name=IshikuraSatoshi
en-aut-sei=Ishikura
en-aut-mei=Satoshi
kn-aut-name=石倉聡
kn-aut-sei=石倉
kn-aut-mei=聡
aut-affil-num=1
ORCID=

en-aut-name=KajiharaShinji
en-aut-sei=Kajihara
en-aut-mei=Shinji
kn-aut-name=梶原真二
kn-aut-sei=梶原
kn-aut-mei=真二
aut-affil-num=2
ORCID=

en-aut-name=FukushimaKeigo
en-aut-sei=Fukushima
en-aut-mei=Keigo
kn-aut-name=福島啓吾
kn-aut-sei=福島
kn-aut-mei=啓吾
aut-affil-num=3
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=広島県立総合技術研究所農業技術センター

affil-num=2
en-affil=
kn-affil=広島県立総合技術研究所農業技術センター

affil-num=3
en-affil=
kn-affil=広島県立総合技術研究所農業技術センター

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=illuminated culture
kn-keyword=illuminated culture
en-keyword=light-emitting diode
kn-keyword=light-emitting diode
en-keyword=long day treatment
kn-keyword=long day treatment
en-keyword=regulation of flowering
kn-keyword=regulation of flowering
en-keyword=spectral quality of light
kn-keyword=spectral quality of light
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=29
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Day Length, Supplemental Lighting Strength, Shading Period and Minimum Night Temperature on Occurrence of Abnormal Inflorescence in Gypsophila paniculata ‘Altair’
kn-title=日長，補光強度，遮光時期および最低夜温がシュッコンカスミソウ ‘アルタイル’の形態異常花序発生に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=シュッコンカスミソウ‘アルタイル’の形態異常花序の発生には環境要因が関与していると考えられたので，日長，補光強度，遮光時期および最低夜温が形態異常花序発生に及ぼす影響を調査した．形態異常程度は４種類のパターン (０：正常，１：茎が短いもの，２：２本の茎が癒着，3：ひどく湾曲し変形したもの) に分類し，その影響を受けた小花の割合を求めた．蛍光灯による日長処理（12時間，16時間，20時間，24時間）や白熱灯による日長処理（自然日長，24時間）は形態異常花序発生率に影響を及ぼさなかった．蛍光灯（PPFD 1μmol・m-2・s-1），白熱灯（PPFD 3μmol・m-2・s-1），メタルハライドランプ（PPFD 14μmol・m-2・s-1），高圧ナトリウムランプ（PPFD 48μmol・m-2・s-1）を用いて16時間の補光を行った．異なる光源による光強度でも形態異常発生率に一定の傾向は認められなかった．遮光時期を変えても形態異常発生率に一定の傾向は認められなかった． 最低夜温を15℃に上げると８℃区と比較して15℃区の形態異常発生は大きく減少した．特にパターン２と３の発生率は大幅に低下した．各実験の処理開始から発蕾までの平均夜温（7.1℃，9.0℃，9.2℃，11.6℃，16.4℃）と，パターン３の形態異常発生率（13.1%，8.7%，7.1%，1.1%，0.7%）との間に高い負の相関（R2＝0.849）が認められ，処理開始から発蕾までの平均夜温が高いほど形態異常発生率は低下した．以上のことから，形態異常花序発生には夜間の温度が大きく関与しているのではないかと推察された．
kn-abstract=As occurrence of abnormal inflorescence in Gypsophila paniculata ‘Altair’ is caused by environmental conditions, effects of day length, supplemental lighting strength, shading period and minimum night temperature on occurrence of abnormal inflorescence were investigated. Abnormal inflorescence was classified into four types : normal, pattern 1 (Short-flower stalk), pattern 2 (Coalescent two-flower stalk) and pattern 3 (Looping and irregular-flower stalk). Neither of 12h, 16h, 20h or 24h day length by fluorescent lamp, nor 24h by incandescent lamp affected occurrence of abnormal inflorescence. Effects of four levels of light intensity (fluorescent lamp : PPFD 1μmol・m−2・s−1, incandescent lamp : PPFD 3μmol・m−2・s−1, metal halide lamp : PPFD 14μmol・m−2・s−1 and high-pressure sodium lamp : PPFD 48μmol・m−2・s−1) were examined in 16h photoperiod. Occurrence of abnormal inflorescence was not affected by different light intensities, neither was it affected by shading period. Occurrence of abnormal inflorescence at 15°C was however significantly reduced compared to that at 8°C. In particular, patterns 2 and 3 at 15°C were significantly reduced compared to those at 8°C. There was a strong negative correlation between average night temperature from starting the treatment to flower budding (7.1°C, 9.0°C, 9.2°C, 11.6°C and 16.4°C) and incidence of pattern 3 (13.1%, 8.7%, 7.1%, 1.1% and 0.7%). Therefore, as average night temperature increased, occurrence of abnormal inflorescence decreased. The results show that low night temperature may be the main factor inducing occurrence of abnormal inflorescence.
en-copyright=
kn-copyright=

en-aut-name=YamaguchiNorihito
en-aut-sei=Yamaguchi
en-aut-mei=Norihito
kn-aut-name=山口訓史
kn-aut-sei=山口
kn-aut-mei=訓史
aut-affil-num=1
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=2
ORCID=

en-aut-name=KobikiKayoko
en-aut-sei=Kobiki
en-aut-mei=Kayoko
kn-aut-name=小日置佳世子
kn-aut-sei=小日置
kn-aut-mei=佳世子
aut-affil-num=3
ORCID=

en-aut-name=OtaniShoko
en-aut-sei=Otani
en-aut-mei=Shoko
kn-aut-name=大谷翔子
kn-aut-sei=大谷
kn-aut-mei=翔子
aut-affil-num=4
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=

affil-num=4
en-affil=
kn-affil=

affil-num=5
en-affil=
kn-affil=岡山大学
en-keyword=abnormal inflorescence pattern
kn-keyword=abnormal inflorescence pattern
en-keyword=cut flower form
kn-keyword=cut flower form
en-keyword=environmental factor
kn-keyword=environmental factor
en-keyword=incidence of abnormal inflorescence
kn-keyword=incidence of abnormal inflorescence
en-keyword=low night temperature
kn-keyword=low night temperature
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=21
end-page=28
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Incidence of Blossom-end Rot in Relation to Water-soluble Ca Concentration in Tomato Fruits as Affected by Ca Nutrition under Root Restriction
kn-title=培養液中Ca濃度が根域制限栽培したトマト果実の 水溶性Ca濃度と尻腐れ果発生に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=根域制限下におけるトマトの養分吸収を調査した結果，培養液中Caの多くは排液とともに排出され，Ｎに対するCaの吸収比率は園試処方やHoagland処方中の比率の50%以下であった．そこで，園試処方培養液中のCa(NO3)2･4H2Oの一部をNH4NO3に置換してCa濃度１，２，４ﾋの培養液を作成し，日射比例給液制御下で ‘ハウス桃太郎’の根城制限栽培（培地容量250，500
㎖）を行った．培養液のCa濃度が１ﾋであっても排液中のCa濃度が２ﾋよりも高く推移したが，Ca吸収量は極めて少なく，著しいCa欠乏症状が現れた．低Ca濃度区では尻腐れ果が多発し，１ﾋ区では第３果房より上段の果実は全て尻腐れ果となり，第９果房までの収量は２ﾋ区，１ﾋ区でそれぞれ４ﾋ区の50%，36%に低下した．腋芽茎中のCa濃度の低下は0.6N-NaCl可溶性画分や0.6N-HCl可溶性画分と比較して水溶性画分の低下が著しかった．そこで，Ca濃度が１～４ﾋの培養液を用いて点滴栽培を行い，果実中のCa濃度を分画して定量した．果実頂部のCa濃度はいずれの画分においても培養液中Ca濃度が低いほど低く，開花から尻腐れ症状が発生するまでの日数も短くなった．尻腐れ果発生率と果頂部の水溶性Ca濃度との間にのみ有意な負の相関が認められた．以上のことから，果実の水溶性Ca濃度がトマトの尻腐れ果発生リスクの大小に大きくかかわっており，植物体の水溶性Ca濃度測定は尻腐れ果発生防止のためのCa栄養診断に応用できる可能性が高いと考えられる．
kn-abstract=The rate of absorbed Ca to N was less than half of Enshi or Hoagland solution in tomato (Solanum lycopersicum Mill.) grown with restricted root zone volume in previous experiments. Tomato plants were grown in plastic pots containing 250 or 500ml of peat based medium with modified Enshi solutions containing 1, 2 or 4mM of Ca. The solutions were prepared by replacing a part of Ca(NO3)2・4H2O to NH4NO3. Although Ca concentration was higher than 2mM in drainage collected from plants supplied 1mM Ca solution, absorbed amount of Ca was very small and severe symptom of Ca deficiency was observed. Incidence of blossom-end rot (BER) increased with decrease in Ca concentration of the solution and yield decreased to 50% and 36% of 4mM control, in 2mM and 1mM plots, respectively. Among fractionated Ca in stem of lateral shoot, decrease in water-soluble fraction was remarkable compared to 1N NaCl- or 0.6N HCl-soluble fractions. Tomato plants were then grown with modified solutions containing 1~4mM of Ca and fractionated Ca was determined for distal half of fruits. With decrease in solution Ca, fruit Ca decreased in all fractions, and days to BER incidence after flowering also decreased. Significant relationship was found only between the water-soluble Ca concentration in fruit tissue and rate of BER incidence. Thus water-soluble Ca in tomato fruit may closely relate to BER incidence, and an efficient tool to estimate the potential risk of BER may possibly be developed by determining the Ca fraction in the stem of lateral shoots.
en-copyright=
kn-copyright=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=1
ORCID=

en-aut-name=ShingaiAya
en-aut-sei=Shingai
en-aut-mei=Aya
kn-aut-name=新開礼
kn-aut-sei=新開
kn-aut-mei=礼
aut-affil-num=2
ORCID=

en-aut-name=OoyamaMitsuo
en-aut-sei=Ooyama
en-aut-mei=Mitsuo
kn-aut-name=大山光男
kn-aut-sei=大山
kn-aut-mei=光男
aut-affil-num=3
ORCID=

en-aut-name=MurakamiKenji
en-aut-sei=Murakami
en-aut-mei=Kenji
kn-aut-name=村上賢治
kn-aut-sei=村上
kn-aut-mei=賢治
aut-affil-num=4
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=

affil-num=3
en-affil=
kn-affil=

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岡山大学
en-keyword=BER
kn-keyword=BER
en-keyword=Ca absorption
kn-keyword=Ca absorption
en-keyword=Ca fractions
kn-keyword=Ca fractions
en-keyword=dripfertigation
kn-keyword=dripfertigation
en-keyword=Enshi solution
kn-keyword=Enshi solution
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=15
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Supplied Amount and Strength of Nutrient Solution on Growth, Yield, and Fruit Quality of Strawberry ‘Saga-honoka’ Grown with Sufficiently Elevated CO2
kn-title=培養液濃度と施用量が高CO2濃度条件下で育てたイチゴ‘さがほのか’の生育・収量と果実品質に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=イチゴ‘さがほのか’を用いて，高CO2濃度条件下(日中800～2500ppm)での栽培における培養液濃度と施用量の影響について検討した．培養液は排液率20%を目標に，大塚Ａ処方を30～50%濃度で適宜変更する慣行区を標準として，濃度をその1.2倍，0.8倍とする高濃度区と低濃度区，さらに低濃度区においては，給液量を1.2倍(排液率35%)とする低濃度多量区を設け，計４処理区とした．排液率20%を目標とした低濃度培養液管理では，12月以降の排液中NO3-Nは検出限界以下であり，栽培期間を通して養分が不足しがちであった．また，全Ｎ施用量も他の３処理区と比較すると少なくなり，１月以降の葉面積が小さくなった．収量・果実品質においては処理区間で有意な差は認められなかったが，チップバーン発生率は，高濃度区で高く，低濃度多量区の発生率が最も低かった．低濃度多量区では，葉面積も大きくなったことから，‘さがほのか’においては，排液率30～40%，排液EC50～60mS・m-1を目標とした低濃度での培養液管理が望ましいといえる．
kn-abstract=The effects of supplied amount and strength of nutrient solution were investigated for strawberry (Fragaria×ananassa Duch. cv. Saga-honoka) grown with peat bags in elevated CO2 environment (800-2500 ppm in the day time, November 4 to April 7). Three strengths (L : low-80%, M : standard-100% and H : high-120%) of nutrient solution (N : 8.85, P : 0.85, K : 3.90, Ca : 2.05, Mg : 0.93 mM ; half strength of Ohtsuka A solution) were compared. Around 20% of discharged rate (discharged/supplied amount of nutrient solution) was kept for these 3 plots and 30 to 40% of the rate was kept for additional plot of 80%-solution (L2) by altering the supplied amount of 80%-solution. As almost no nitrate could be detected in drainage of L, nutrient supply was probably insufficient throughout the experiment. Total amount of nitrogen supply was lower than the other 3 plots and leaf area was the smallest after December. Although there was no significant difference in yield and fruit quality, the rate of tip burn affected flowers was lowest in L2 and highest in H. Thus, around 30-40% of drainage rate and 50-60 mS・m−1 of drainage EC may be desirable target values for ‘Saga-honoka’ strawberry grown with peat based substrate.
en-copyright=
kn-copyright=

en-aut-name=InazumiDaichi
en-aut-sei=Inazumi
en-aut-mei=Daichi
kn-aut-name=稲角大地
kn-aut-sei=稲角
kn-aut-mei=大地
aut-affil-num=1
ORCID=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=2
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=3
ORCID=

en-aut-name=MurakamiKenji
en-aut-sei=Murakami
en-aut-mei=Kenji
kn-aut-name=村上賢治
kn-aut-sei=村上
kn-aut-mei=賢治
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=nutrient absorption
kn-keyword=nutrient absorption
en-keyword=tipburn
kn-keyword=tipburn
en-keyword=titratable acid
kn-keyword=titratable acid
en-keyword=total soluble solid
kn-keyword=total soluble solid
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=7
end-page=14
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A Volatile Substance, β-Caryophyllene, from Talaromyces wortmannii Promotes Growth and Tolerance to Diseases on Several Plants
kn-title=β-caryophylleneの植物に対する生育促進作用 および耐病性増進作用の解析
en-subtitle=
kn-subtitle=
en-abstract=岡山県総社市の圃場から分離した植物生育促進菌Talaromyces wortmannii FS2が生産するβ-caryophylleneは，コマツナ（アブラナ科）のみならず，キュウリ（ウリ科），タバコ（ナス科）およびオオムギ（イネ科）など広汎な植物に対して，生育促進作用および耐病性増進作用を示したことから，有用な農業資材として利用可能であるものと考察した．
kn-abstract=A plant growth-promoting fungus, Talaromyces wortmannii strain FS2 was isolated from an agricultural field at Okayama Pref. FS2 enhanced seed germination, root elongation and leaf growth of Brassica rapa var perviridis (Komatsuna). Such plant growth-promoting effect was observed in the same sealed chamber where FS2 was cultured on PDA medium separated from seedlings, suggesting effective volatile compound(s). GC‒MS analysis showed that FS2 emitted at least seven terpenoids, of which a volatile was identified as β‒caryophyllene. β‒caryophyllene alone promoted the growth of cucumber, Nicotiana benthamiana and barley. Furthermore β‒caryophyllene increased the yield of cucumber fruits. Interestingly, we found that β‒caryophyllene conditioned these plants to be resistant to respective diseases caused by Colletotrichum orbiculare, Botrytis cinerea or Blumeria graminis f. sp hordei. The findings indicate that β‒caryophyllene has desirable dual features and therefore, it is available to cultivation of many crops.
en-copyright=
kn-copyright=

en-aut-name=YamagiwaYasuo
en-aut-sei=Yamagiwa
en-aut-mei=Yasuo
kn-aut-name=山際泰夫
kn-aut-sei=山際
kn-aut-mei=泰夫
aut-affil-num=1
ORCID=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=豊田和弘
kn-aut-sei=豊田
kn-aut-mei=和弘
aut-affil-num=2
ORCID=

en-aut-name=InagakiYoshishige
en-aut-sei=Inagaki
en-aut-mei=Yoshishige
kn-aut-name=稲垣善茂
kn-aut-sei=稲垣
kn-aut-mei=善茂
aut-affil-num=3
ORCID=

en-aut-name=IchinoseYuki
en-aut-sei=Ichinose
en-aut-mei=Yuki
kn-aut-name=一瀬勇規
kn-aut-sei=一瀬
kn-aut-mei=勇規
aut-affil-num=4
ORCID=

en-aut-name=HyakumachiaMitsuro
en-aut-sei=Hyakumachia
en-aut-mei=Mitsuro
kn-aut-name=百町満朗
kn-aut-sei=百町
kn-aut-mei=満朗
aut-affil-num=5
ORCID=

en-aut-name=ShiraishiTomonori
en-aut-sei=Shiraishi
en-aut-mei=Tomonori
kn-aut-name=白石友紀
kn-aut-sei=白石
kn-aut-mei=友紀
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岐阜大学大学院

affil-num=6
en-affil=
kn-affil=岡山大学
en-keyword=β-caryophyllene
kn-keyword=β-caryophyllene
en-keyword=plant growth-promoting
kn-keyword=plant growth-promoting
en-keyword=resistance induction
kn-keyword=resistance induction
en-keyword=Talaromyces wortmannii
kn-keyword=Talaromyces wortmannii
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Isolation and Identification of a Plant Growth-Promoting Fungus from an Agricultural Field in Okayama Prefecture
kn-title=岡山県の栽培圃場における植物生育促進菌の探索と同定
en-subtitle=
kn-subtitle=
en-abstract=本研究では，実際の生産圃場から植物生育促進菌（PGPF）の探索を試み，コマツナの生育を促進するFS2株を分離した．FS2株の形態観察並びにのITS1領域の系統樹解析から本菌をTalaromyces wortmanniiと同定した．
kn-abstract=A plant growth-promoting fungus was isolated from an agricultural field in Okayama Prefecture, Japan. The strain FS2, which enhanced seed germination, root elongation and leaf growth of Brassica rapa var. perviridis, was identified as Talaromyces wortmannii based on ITS1 sequence and its morphology.
en-copyright=
kn-copyright=

en-aut-name=YamagiwaYasuo
en-aut-sei=Yamagiwa
en-aut-mei=Yasuo
kn-aut-name=山際泰夫
kn-aut-sei=山際
kn-aut-mei=泰夫
aut-affil-num=1
ORCID=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=豊田和弘
kn-aut-sei=豊田
kn-aut-mei=和弘
aut-affil-num=2
ORCID=

en-aut-name=InagakiYoshishige
en-aut-sei=Inagaki
en-aut-mei=Yoshishige
kn-aut-name=稲垣善茂
kn-aut-sei=稲垣
kn-aut-mei=善茂
aut-affil-num=3
ORCID=

en-aut-name=IchinoseYuki
en-aut-sei=Ichinose
en-aut-mei=Yuki
kn-aut-name=一瀬勇規
kn-aut-sei=一瀬
kn-aut-mei=勇規
aut-affil-num=4
ORCID=

en-aut-name=ShiraishiTomonori
en-aut-sei=Shiraishi
en-aut-mei=Tomonori
kn-aut-name=白石友紀
kn-aut-sei=白石
kn-aut-mei=友紀
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岡山大学
en-keyword=Brassica rapa var. perviridis (Komatsuna)
kn-keyword=Brassica rapa var. perviridis (Komatsuna)
en-keyword=ITS1 region
kn-keyword=ITS1 region
en-keyword=Plant growth-promoting fungus
kn-keyword=Plant growth-promoting fungus
en-keyword=Talaromyces wortmannii (Penicillium kloeckeri)
kn-keyword=Talaromyces wortmannii (Penicillium kloeckeri)
en-keyword=volatile compounds
kn-keyword=volatile compounds
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2013
dt-pub=20130201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=裏表紙・英文目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=奥付
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=71
end-page=83
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Published by Members of the Faculty From January to December 2011
kn-title=公表学術論文等リスト 2011
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=65
end-page=70
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Research into food preservation using a bacteriocin produced by Lactobacillus gasseri
kn-title=Lactobacillus gasseri の生産するバクテリオシンの食品利用へ向けた検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bacteriocins are natural antibacterial peptides ribosomally biosynthesized by bacteria. Gassericin T which is a bacteriocin produced by many strains of Lactobacillus gasseri has a broad antibacterial spectrum against food spoilage and pathogenic Gram-positive bacteria; therefore, the bacteriocin and its producers are predicted for use as safe food preservatives. However, probiotic Lb. gasseri strains show poor growth and minimally produce gassericin T in food-grade
natural media such as reconstituted cheese whey, unlike in MRS broth. The growth of Lb. gasseri in reconstituted cheese whey was improved by adding proteose peptone as a nutrient factor. The production of gassericin T in MRS broth was specifically inhibited by adding divalent metal cations, and then the inhibition was removed using a chelator of divalent cations, trisodium citrate dihydrate. The production of gassericin T in reconstituted cheese whey was also restored by adding trisodium citrate dihydrate, and the activity was enhanced by a surfactant, Tween 80. In addition, trisodium citrate dihydrate led to over-production of and synergistic antibacterial effect with gassericin T. In this study, we developed a cheese whey-based medium containing proteose peptone, trisodium citrate dihydrate and Tween 80 for gassericin T production. The developed food-grade medium may contribute to the effective use of some bacteriocins from probiotic lactic acid bacteria for biopreservation of foods.
en-copyright=
kn-copyright=

en-aut-name=ArakawaKensuke
en-aut-sei=Arakawa
en-aut-mei=Kensuke
kn-aut-name=荒川健佑
kn-aut-sei=荒川
kn-aut-mei=健佑
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
en-keyword=Bacteriocin
kn-keyword=Bacteriocin
en-keyword=Lactobacillus gasseri
kn-keyword=Lactobacillus gasseri
en-keyword=Gassericin T
kn-keyword=Gassericin T
en-keyword=Biopreservation
kn-keyword=Biopreservation
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=49
end-page=64
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Application of continuous light in a plant factory system 4. Physiological changes and concept of injury induction in plant leaves under continuous light
kn-title=閉鎖型植物工場における連続光の利用（第4報）連続光下における植物の生理学的変化ならびに障害誘発の概念
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Physiological changes and concept of injury induction occurring under continuous light are comprehensively reviewed. Continuous light usually reduces photosynthetic rate, which may relate to changes in transpiration and leaf necrosis caused by reactive oxygen species. Other factors apart from photosynthesis may also affect leaf injuries occurring under continuous light. Continuous light sometimes increases carbohydrate and some secondary metabolite contents.
en-copyright=
kn-copyright=

en-aut-name=HataNaoki
en-aut-sei=Hata
en-aut-mei=Naoki
kn-aut-name=畑直樹
kn-aut-sei=畑
kn-aut-mei=直樹
aut-affil-num=1
ORCID=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=2
ORCID=

en-aut-name=MurakamiKenji
en-aut-sei=Murakami
en-aut-mei=Kenji
kn-aut-name=村上賢治
kn-aut-sei=村上
kn-aut-mei=賢治
aut-affil-num=3
ORCID=

en-aut-name=KobayashiAkio
en-aut-sei=Kobayashi
en-aut-mei=Akio
kn-aut-name=小林昭雄
kn-aut-sei=小林
kn-aut-mei=昭雄
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=4
en-affil=
kn-affil=大阪大学大学院工学研究科
en-keyword=carbohydrates
kn-keyword=carbohydrates
en-keyword=circadian rhythm
kn-keyword=circadian rhythm
en-keyword=ethylene
kn-keyword=ethylene
en-keyword=photosynthesis
kn-keyword=photosynthesis
en-keyword=reactive oxygen species
kn-keyword=reactive oxygen species
en-keyword=secondary metabolites
kn-keyword=secondary metabolites
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=39
end-page=47
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=インドネシア，西ジャワのマンゴスチン園における気象環境と葉のC-N 率の樹冠部位および樹齢による違い
kn-title=Meteorological Conditions of Mangosteen Orchard in West Java, Indonesia and Seasonal Changes in C-N Ratio of Their Leaves as Affected by Sector (Position in Canopy) and Tree Age
en-subtitle=
kn-subtitle=
en-abstract=マンゴスチンの果実生産性や果実品質が樹冠部位や樹齢によって異なる点について，インドネシア西ジャワの商業的マンゴスチン園で照度と降雨量を調査するとともに，20，35および50年生樹を５個体ずつ選び，各個体樹冠の高さと幅から9つのセクター（樹冠部位）に分け，葉の葉色値（SPAD値），クロロフィル含量，炭水化物含量，窒素含量およびC-N率を比較した．晴天日の照度は，樹齢による差は小さくセクター9（各樹齢7.5～8.1μmol・M(-2)・S(-1)）が最も高く，一方セクター1（各樹齢0.4～0.5μmol・M(-2)・S(-1)）で最も低かった．樹冠外部の照度はいずれの樹齢も樹冠内部よりも高く，またセクター1，2，3のような下部よりも7，8，9のような上部で高かった．調査を行った2010年は雨季の開始が例年よりも2～3か月早く，このため樹体の生育相が例年とは大きく異なった．葉色値は樹齢に関係なくセクター1～4のような下部の葉よりもセクター5～9のような上部の葉で高かった．葉の炭水化物含量は，20年生樹ではセクター1～3の下部よりも7～9の上部で高かったが，35年生と50年生樹ではそれぞれ50.7～53.0%と51.3～52.6%で大差なかった．一方，葉の窒素含量は樹齢や測定時期に関係なくセクター5～9の上部よりもセクター1～4の下部で高かった．このため，葉のC-N率は樹冠の下部よりも上部で高かった．この結果をもとにマンゴスチンの果実生産性と品質および樹冠部位と樹齢との関係を考察した．
kn-abstract=Productivity and quality of mangosteen fruit is markedly affected by tree age and sector (position in canopy). The objective of this study was to make clear seasonal changes in meteorological conditions of mangosteen orchard, and C-N ratio of leaves in relation to tree age and sector. The experiment was conducted using mangosteen trees grown in commercial orchard in Bogor, Indonesia during May to October 2010. Mangosteen trees of 3 different ages, young (20-year-old), middle (35-year-old), and old
(50-year-old), each of five trees, were selected for study, and the canopy of each tree was divided into 9 sectors based on height (bottom, middle, top) and width (inner, center, outer). The light intensity on sunny days was higher in Sector 9, it was 8.1, 7.5, and 7.7 μmol・M(−2)・s(−1) in young, middle-aged and old trees, respectively, whereas in Sector 1 it was low, resulting 0.5, 0.4, and 0.5 μmol・M(−2)・s(−1) in young, middle-aged and old trees, respectively. Regardless of tree age, light intensity in outer position in canopy was higher than in the inner, and also it was higher in taller than in lower positions in canopy. In 2010, the phenological aspects of mangosteen orchard markedly changed because of irregular rainfall. In spite of tree age, SPAD value of leaves in upper parts such as Sectors 5-9 was high comparing with lower ones such as Sectors 1-4. In young trees, carbohydrate content of leaves was higher in top positions such as Sectors 7-9 than in bottom ones such as Sectors 1-3, whereas in middle-aged and old trees, no significant difference of carbohydrate content was observed among sectors. Regardless of month and tree age measured, nitrogen content of leaves was higher in lower positions in canopy such as Sectors 1-4 than in upper ones such as Sectors 5-9. Consequently, the C-N ratio of leaves was higher in the upper part of canopy compared to the lower. Based on the results, the relationships between meteorological status and C-N ratio of leaves and fruit productivity and quality of mangosteen are discussed in
relation to position in canopy and tree age.
en-copyright=
kn-copyright=

en-aut-name=SetiawanEko
en-aut-sei=Setiawan
en-aut-mei=Eko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=PoerwantoRoedhy
en-aut-sei=Poerwanto
en-aut-mei=Roedhy
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=FukudaFumio
en-aut-sei=Fukuda
en-aut-mei=Fumio
kn-aut-name=福田文夫
kn-aut-sei=福田
kn-aut-mei=文夫
aut-affil-num=3
ORCID=

en-aut-name=KubotaNaohiro
en-aut-sei=Kubota
en-aut-mei=Naohiro
kn-aut-name=久保田尚浩
kn-aut-sei=久保田
kn-aut-mei=尚浩
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Faculty of Agriculture, Bogor Agricultural University

affil-num=3
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
en-keyword=chlorophyll
kn-keyword=chlorophyll
en-keyword=C-N ratio
kn-keyword=C-N ratio
en-keyword=meteorological conditions
kn-keyword=meteorological conditions
en-keyword=sector (position in canopy)
kn-keyword=sector (position in canopy)
en-keyword=tree age
kn-keyword=tree age
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=33
end-page=37
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Air or Medium Temperature on Occurrence of Leaf-yellow-spot in Chrysanthemum 'Seikou-no-makoto'
kn-title=気温および地温がキク‘精興の誠’の黄斑発生に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=キク（Chrysanthemum × morifolium）‘精興の誠’の葉身で発生する黄斑に及ぼす気温および培地温度の影響を調査した．昼夜温を40/30℃，35/25℃，なりゆきの3区で栽培した場合，昼夜温が高いほど生育は抑制され，黄斑の発生は早くなったが，黄斑発生度は40/30℃区と比較してなりゆき区で高くなる傾向がみられた．夜温を30℃，25℃となりゆき区で栽培した場合，黄斑発生度は30℃区と比較してなりゆき区で有意な差がみられた．生育が抑制されるほどの長期間の強光や高温は黄斑発生を抑制させたことから，生育が旺盛な環境条件における外的要因により黄斑発生は助長されると考えられた．地下部の温度は黄斑発生に影響しなかったことから黄斑発生は地上部，特に葉身部位の高温により助長されることが明らかになった．
kn-abstract=Leaf-yellow-spot, a physiological abnormality occurring in leaves of several chrysanthemum (Chrysanthemum x morifolium) cultivars is a very serious production problem in Japan. High temperature or high irradiation are possible physiological factors, which may lead to leaf-yellow-spot. In the present study, effects of air or medium temperature on the occurrence of leaf-yellow-spot in 'Seikou-nomakoto' were investigated. The nodal position with spotted leaves and rate of leaf-yellow-spot increased
with increasing day/night temperature. The nodal position with spotted leaves and rate of leaf-yellow-spot of plants grown on 30°C night air temperature were smaller than those grown on 25°C or ambient night air temperature. The days to first occurrence of leaf-yellow-spot showed no differences among medium temperatures. As occurrence of leaf-yellow-spot was not affected by root zone temperature, we recognized that occurrence of leaf-yellow-spot was promoted by high temperature or solar radiation on shoot, especially leaf. Occurrence of leaf-yellow-spot was reduced by long period high temperature and/or high solar radiation as plant growth reduced. Therefore, we thought that occurrence of leaf-yellow-spot was promoted by environmental condition as plant growth would promote.
en-copyright=
kn-copyright=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=1
ORCID=

en-aut-name=YamaguchiNorihito
en-aut-sei=Yamaguchi
en-aut-mei=Norihito
kn-aut-name=山口訓史
kn-aut-sei=山口
kn-aut-mei=訓史
aut-affil-num=2
ORCID=

en-aut-name=FujitaShino
en-aut-sei=Fujita
en-aut-mei=Shino
kn-aut-name=藤田紫乃
kn-aut-sei=藤田
kn-aut-mei=紫乃
aut-affil-num=3
ORCID=

en-aut-name=YotsuyaRyosuke
en-aut-sei=Yotsuya
en-aut-mei=Ryosuke
kn-aut-name=四谷亮介
kn-aut-sei=四谷
kn-aut-mei=亮介
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=

affil-num=3
en-affil=
kn-affil=

affil-num=4
en-affil=
kn-affil=
en-keyword=air temperature
kn-keyword=air temperature
en-keyword=environmental factor
kn-keyword=environmental factor
en-keyword=irradiation
kn-keyword=irradiation
en-keyword=leaf-yellow-spot
kn-keyword=leaf-yellow-spot
en-keyword=medium temperature
kn-keyword=medium temperature
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=25
end-page=32
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of shading and high-temperature treatments on appearance quality of brown rice and palatability of cooked rice
kn-title=水稲玄米の外観品質と米飯の食味に及ぼす高温・遮光処理の影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We examined the appearance quality and palatability of milled rice for each grain-thickness group. The rice cultivars, Nipponbare and Hinohikari, were cultivated at the paddy field of Field Science Center, Okayama University in 2009. For high-temperature treatment, both sides of the transparent chamber were opened when the temperature exceeded 36 degrees C, and closed when lower than 25 degrees C, and 50% shading treatment with black cloth was applied during the grain-filling period. After harvesting, the grains of brown rice were sorted according to thickness using a rice grader, and classified
into eight grain thickness groups, less than 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, and over 2.2mm. The appearance quality of grains was measured, and milled grains were cooked, and the palatability and physical properties were measured. The brown rice yield in high-temperature plots was 10-21% less than control due to the decrease in the ripening percentage and 1000-grains weight. Yield in the shading plot dropped to 16-24% less than control mainly due to the decrease in the ripening percentage. The percentage occupation of grains with 1.8-1.9mm thickness was higher in Hinohikari than Nipponbare. In high-temperature plots, thickness of 1.8-2.0mm decreased and thickness of higher than 2.0mm increased in both cultivars. The shading treatment increased thickness of lower than 1.9mm markedly. It was
cleared that the shading decreased grain thickening growth and grains was distributed more among thinner groups, and high-temperature promoted the grain thickening growth despite the decrease in 1000-grains weight. In both cultivars, the lower the grain thickness group, the lower the percentage of perfect grain due to the increase in the percentage of chalky grain. The palatability value of milled rice
was greater in Hinohikari than Nipponbare. The lower the grain thickness group, the lower the palatability value and stickiness, and those tendencies were remarkable in Nipponbare than Hinohikari. The shading and high-temperature treatments decreased the palatability value and the stickiness, especially in Nipponbare. The palatability value decreased with lower the grain thickness group due to an increase in the percentage occupation of chalky grains.
en-copyright=
kn-copyright=

en-aut-name=IshizukiYuuki
en-aut-sei=Ishizuki
en-aut-mei=Yuuki
kn-aut-name=石突裕樹
kn-aut-sei=石突
kn-aut-mei=裕樹
aut-affil-num=1
ORCID=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院自然科学研究科
en-keyword=Chalky rice grains
kn-keyword=Chalky rice grains
en-keyword=Grain thickness group
kn-keyword=Grain thickness group
en-keyword=High-temperature
kn-keyword=High-temperature
en-keyword=palatability
kn-keyword=palatability
en-keyword=Shading
kn-keyword=Shading
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=19
end-page=24
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Pericarp Characteristics of the F(1) Hybrid Medium-Fruited Tomato between the Male Sterile Mutant (T-4) of the Large-Fruited 'First' and a Small-Fruited Pure Line with Soft Pericarp
kn-title=‘ファースト’雄性不稔突然変異体 (T -4) を種子親とした軟果皮中玉トマトF(1) 系統 (MS-II) の特性
en-subtitle=
kn-subtitle=
en-abstract=薄皮果柔の中玉トマト品種育成を目的とし，‘ファースト’花粉非崩壊型雄性不稔系統 (T-4) を種子親，果皮の軟らかい小果固定系統 (S) を花粉親とするF(1)雑種 (MS-II) の特性について，両親系統および既存の中玉F(1)品種‘レッドオーレ’，‘フルティカ’のそれと比較した．両親系統と比較したところ，MS-IIの果実硬度は花粉親である軟果皮Sと同等となり，果皮硬度はSよりも低い値となった．3段摘心栽培において，MS-IIの果実硬度は第1段では2品種と差は無かったが，上位果房ほど両品種よりも低くなる傾向を示した．果皮硬度は，第1果房では‘レッドオーレ’よりも低く，‘フルティカ’よりも高かったが，上位果房ほど両品種よりも低い値となる傾向を示した．MS-IIの外果皮におけるクチクラ厚を測定したところ，‘フルティカ’と同等となり，‘レッドオーレ’よりも低かった．また，MS-IIのクチクラ層の発達程度が2品種よりも低いことが観察され，MS-IIの果皮硬度が2品種よりも低い傾向を示すのは，外果皮におけるクチクラ層の発達程度が低いためと推測された．
kn-abstract=Breeding for a soft pericarp in medium-sized tomato fruit was conducted by crossing the male sterile mutant (T-4) of the large-fruited 'First' and a small-fruited pure line with a soft pericarp (S). Pericarp characteristics of the F(1) hybrid (named MS-II) were compared with the parents and two similar medium-fruited tomato cultivars, 'Red ore' and 'Frutica'. Pericarp firmness in MS-II was lower as compared with that of both T-4 and S. Differences in pericarp firmness among MS-II, 'Red ore' and 'Frutica' were dependent on truss. In the first truss, MS-II developed fruits with a softer pericarp than 'Red ore', but with a firmer pericarp than 'Frutica'. In the second and third trusses, pericarp firmness of the fruit in MS-II tended to be lower than those of the other two cultivars. The thickness of the exocarp cuticle in MS-II was lower than that in 'Red ore', but was no different to that in 'Frutica'. Thus genotypic differences in pericarp firmness among MS-II, 'Red ore' and 'Frutica' seem to be derived from differences in the degree of cutin development in the epidermal perimeter. A thinner cuticle can explain pericarp softness in the fruits above the second truss in MS-II.
en-copyright=
kn-copyright=

en-aut-name=ToyaJunko
en-aut-sei=Toya
en-aut-mei=Junko
kn-aut-name=遠矢純子
kn-aut-sei=遠矢
kn-aut-mei=純子
aut-affil-num=1
ORCID=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=2
ORCID=

en-aut-name=MurakamiKenji
en-aut-sei=Murakami
en-aut-mei=Kenji
kn-aut-name=村上賢治
kn-aut-sei=村上
kn-aut-mei=賢治
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=（現）兵庫県光都農林水産振興事務所

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=cuticle
kn-keyword=cuticle
en-keyword=fruit firmness
kn-keyword=fruit firmness
en-keyword=pericarp firmness
kn-keyword=pericarp firmness
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=13
end-page=18
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Possibility of Sand Culture for Melon Using Root-proof Capillary Wick in Mid-summer Period
kn-title=盛夏期における防根給水ひもを用いたメロン砂栽培の可能性
en-subtitle=
kn-subtitle=
en-abstract=栽培期間の大半の日数が高温となる盛夏期において防根給水ひもを用いたメロン砂栽培が可能であるかどうかを検討した．処理区は，大塚A処方培養液のECを段階的に低下させて供給する培養液区，全量を肥料調節型肥料で培地に混和する肥効調節型肥料区（以下、肥料区）の2区とした．最高平均地温は34.8℃，最低平均地温は26.9℃であった．肥料区では定植2週間後の交配期に8株中5株に萎れ症状が生じたが，これは定植後～交配期において培地が高EC状態であったことが原因と考えら
れた．一方で，培養液区では萎れ症状は全くみられなかった．肥料区の正常３個体と培養液区の全個体の果実重の平均は2.3 ㎏，糖度 (Brix°) は14.4であった．以上より，盛夏期における防根給水ひもを用いたメロン砂栽培は，培養液管理によって安定生産が見込めること，肥料での栽培も施肥設計を考慮すれば同期の砂栽培に適用可能であることが示唆された．
kn-abstract=Effects of liquid fertilizer (LF) and controlled-release fertilizer (CRF) on growth and fruit enlargement of netted melon in 6L sand medium were investigated in a cultivation method using root-proof capillary wicks in mid-summer. Average of maximum and minimum root-zone temperatures was
34.8°Cand 26.9°C, respectively. Wilted symptoms appeared very often in 5 out of 8 plants at 2 weeks after transplanting in CRF with a high electric conductivity (EC) of medium solution. In contrast, such symptoms were not found at all in LF with a lower EC. In LF, average fruit weight was 2.3Kg and fruit Brix was 14.4, and non-wilted plants in CRF also showed the same levels as LF in weight and Brix of fruits. These results indicated the possibility of sand culture for melon in mid-summer with LF or a modified fertilizer combination design of CRF.
en-copyright=
kn-copyright=

en-aut-name=KawaharaMasaki
en-aut-sei=Kawahara
en-aut-mei=Masaki
kn-aut-name=川原雅規
kn-aut-sei=川原
kn-aut-mei=雅規
aut-affil-num=1
ORCID=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=capillary watering
kn-keyword=capillary watering
en-keyword=liquid fertilizer
kn-keyword=liquid fertilizer
en-keyword=controlled-release fertilizer
kn-keyword=controlled-release fertilizer
en-keyword=high temperature
kn-keyword=high temperature
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=7
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The Effect of Prevention of Root Injury on Growth, Development and Dry Weight in Rice (Oryza sativa L.) Transplanted to Saline Soil
kn-title=根損傷の軽減が塩土壌における移植イネの生育と乾物重におよぼす影響
en-subtitle=
kn-subtitle=
en-abstract=イネの根は吸水するとともにナトリウムイオンを排除する役割を持っている．水田移植栽培において根の損傷を防ぐことが効果的に塩害を軽減するかどうかを検討した．耐塩性の弱いイネ極早生品種アキヒカリを用い，土壌を詰めたビニール袋で育苗した．苗の根を5 ㎜～10 ㎜ 残し切除して，塩化ナトリウム（NaCl）0ｇ，7ｇ，10ｇを土壌に添加したポットに移植し，根を傷つけないように移植した植物と生育と成熟期の乾物重を比較した．移植は播種後12日，19日，26日，33日の4回行った．茎数の増加は根の切除および塩添加によって抑制されたが，二要因の交互作用はなかった．成熟期の地上部と穂の乾物重は塩添加によって減少したが，根切除の影響はなかった．穂の乾物重の減少は穂数と平均1籾重が変わらなかったため，平均1穂籾数の低下による平均1穂重の低下によっていた．以上の結果から耐塩性が小さい日本の品種において移植時の根の損傷を防ぐことは，塩害軽減に効果的でないと考えられた．
kn-abstract=Rice root has an important role in water absorption and exclusion of sodium ion in saline soil. However, it is injured during transplanting to paddy field. The objective of this study was to examine whether prevention of such root injury reduces salinity damage in rice. Extremely early rice cultivar "Akihikari" that is not salt tolerant was grown in plastic bags filled with soil. Roots of seedlings were clipped leaving 5mm~10mm, then transplanted to 5L pots filled with soil applied with 0g, 7g and 10g of sodium chloride (NaCl). The growth and dry weight at the mature stage was compared to those of plants transplanted with intact roots. Time of transplanting was 12, 19, 26 and 33 days after sowing. Increase of number of stems was suppressed due to root clipping and salinity, but there was no interaction between the two factors. Dry weight of total above ground part and panicle decreased due to salinity though root clipping did not change them. The reduction of panicle dry weight was caused by the reduction of mean number of spikelets per panicle, thus mean panicle dry weight, as number of panicles and mean spikelet dry weight showed no changes. It was concluded that prevention of the root injury may not effectively alleviate salinity damage in less salt tolerant rice cultivars.
en-copyright=
kn-copyright=

en-aut-name=TsudaMakoto
en-aut-sei=Tsuda
en-aut-mei=Makoto
kn-aut-name=津田誠
kn-aut-sei=津田
kn-aut-mei=誠
aut-affil-num=1
ORCID=

en-aut-name=UedaYukie
en-aut-sei=Ueda
en-aut-mei=Yukie
kn-aut-name=上田裕紀枝
kn-aut-sei=上田
kn-aut-mei=裕紀枝
aut-affil-num=2
ORCID=

en-aut-name=HiraiYoshihiko
en-aut-sei=Hirai
en-aut-mei=Yoshihiko
kn-aut-name=平井儀彦
kn-aut-sei=平井
kn-aut-mei=儀彦
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=Rice (Oryza sativa L.)
kn-keyword=Rice (Oryza sativa L.)
en-keyword=Root clipping
kn-keyword=Root clipping
en-keyword=Salinity
kn-keyword=Salinity
en-keyword=Tillering
kn-keyword=Tillering
en-keyword=Transplantation
kn-keyword=Transplantation
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Purification and Characterization of Thermostable Amidase from Thermus sp.O-3-1
kn-title=好熱性細菌Thermus sp.O-3-1由来耐熱性アミダーゼの精製及び性質検討
en-subtitle=
kn-subtitle=
en-abstract=好熱性細菌Thermus sp.O-3-1 由来の耐熱性アミダーゼ遺伝子を大腸菌中にクローニングし，その塩基配列を決定した．ami 遺伝子は930 bp からなり，310アミノ酸をコードしていた．本酵素の分子量は33，089 Daであると予想された．Thermus sp.O-3-1 由来アミダーゼを大腸菌で生産させ，熱処理とDEAE-トヨパール650M陰イオン交換カラム等により精製した．ゲル濾過クロマトグラフィーとSDS-PAGE の結果から本酵素は分子質量33 kDa のサブユニット2分子からなるダイマー構造を有していることが明らかとなった．精製酵素の熱安定性は80℃まで，pH 安定性は7.0～10.0であり，安定性の
高い酵素であった．最適温度は90℃，最適 pH は9.0であ
った．EDTA により活性が著しく阻害され，Co(2+)やNi(2+)，Mn(2+)によって活性の回復，向上が見られたため，本酵素は金属酵素であることが示唆された．基質特異性の検討
の結果，L-Leu-pNA よりもD-Leu-pNA に対して高い活性を示したため，本酵素がＤ-アミノ酸基質に特異性を持つアミダーゼであることが判明した．本酵素は耐熱性を有するユニークなＤ-アミノ酸アミダーゼであり，今後産業利用が期待される．
kn-abstract=The gene encoding a thermostable amidase (EC 3.5.1.4) from thermophilic bacterium Thermus sp.O-3-1, was cloned and expressed in Escherichia coli JM109. The cloned amidase gene (ami) is 930 bp and encodes a protein composed of 310 amino acids. The protein is predicted to have a molecular mass of 33,089 Da. The amidase from Thermus sp.O-3-1 was purified by heat treatment and DEAE Toyopearl 650M column chromatography. The molecular mass of the native enzyme was estimated to be about 70 kDa by gel filtration chromatography, indicating that the enzyme has a homodimeric structure. The purified enzyme was stable up to 80°C and within a pH range from 7.0 to 10.0. The optimum temperature and pH for enzyme activity were 90°C, and 9.0, respectively. The enzyme was strongly inhibited by the metal-chelating compound EDTA. The activity of the EDTA-treated enzyme was reactivated by the addition of Co(2+), Ni(2+) and Mn(2+) ions. Therefore the enzyme was predicted to be metalloenzyme. Finally,
as a result of investigation into substrate specificity, the purified enzyme was suggested to be D-amino acid specific amidase, as it showed higher activity toward D-Leu-pNA than L-Leu-pNA.
en-copyright=
kn-copyright=

en-aut-name=KobayashiFumiaki
en-aut-sei=Kobayashi
en-aut-mei=Fumiaki
kn-aut-name=小林史明
kn-aut-sei=小林
kn-aut-mei=史明
aut-affil-num=1
ORCID=

en-aut-name=AomineHiroki
en-aut-sei=Aomine
en-aut-mei=Hiroki
kn-aut-name=青峰弘起
kn-aut-sei=青峰
kn-aut-mei=弘起
aut-affil-num=2
ORCID=

en-aut-name=MizunashiWataru
en-aut-sei=Mizunashi
en-aut-mei=Wataru
kn-aut-name=水無渉
kn-aut-sei=水無
kn-aut-mei=渉
aut-affil-num=3
ORCID=

en-aut-name=YuFujio
en-aut-sei=Yu
en-aut-mei=Fujio
kn-aut-name=湯不二夫
kn-aut-sei=湯
kn-aut-mei=不二夫
aut-affil-num=4
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=5
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=

affil-num=2
en-affil=
kn-affil=

affil-num=3
en-affil=
kn-affil=(株)三菱レイヨン

affil-num=4
en-affil=
kn-affil=

affil-num=5
en-affil=
kn-affil=岡山大学

affil-num=6
en-affil=
kn-affil=岡山大学
en-keyword=amidase
kn-keyword=amidase
en-keyword=thermostable enzyme
kn-keyword=thermostable enzyme
en-keyword=Thermus
kn-keyword=Thermus
en-keyword=D-amino acid specific amidase
kn-keyword=D-amino acid specific amidase
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=目次
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=101
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=表紙
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=67
end-page=81
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Papers Published by Members of the Faculty from January to December 2010
kn-title=公表学術論文等リスト　2010
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=Faculty of Agriculture, Okayama University
en-aut-sei=Faculty of Agriculture, Okayama University
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=61
end-page=65
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Estimation of genetic parameters for beef marbling score and marbling on top round of Japanese Black
kn-title=黒毛和牛におけるBMSおよび モモヌケの遺伝的パラメータの推定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Japanese Black cattle are the predominant beef breed in Japan. This breed has excellent meat quality, especially degree of (intramuscular) marbling. Degree of marbling was measured at the 6th to 7th rib section and according to the Beef Marbling Standard with scores 1 to 12 (so-called BMS), with number 12 being the best. BMS is used with business deal of beef carcass and has strongly effect on selling price of carcass. On the other hands, more degree of marbling on top round (MTR) is better carcass unit price in same BMS, because of good price of part of thigh. MTR has been focused in recent years. However, MTR is unclear how to evaluate, therefore, collection of records and genetic evaluations are not enough. In this study, records of MTR were collected and then, genetic parameters for BMS and MTR of Japanese Black were estimated. The original data were 11,052 carcass records collected from April in 2008 to June in 2009 at the Agura Farm. Only information from abattoirs having more than 20 records and feedlot farms having more than 10 records were used. This resulted in a total of 10,990 records. In this study, pedigree information was traced back to two generations. An animal model that included all relationships among all animals was used as a statistical model. The fixed effects included sex, farm, abattoir and the combination of slaughter year and month. Slaughter age was fitted as linear covariables. Genetic parameters were estimated using REMLF90 and THRGIBBS1F90 programs. The estimates of heritability were moderately high (0.37 to 0.44) for MTR treated as threshold traits. The estimates of phenotypic and genetic correlations between BMS and MTR were very high (0.87 and 0.98, respectively). These indicate that MTR can be improved genetically and improvement of MTR can treat BMS as indicator.
en-copyright=
kn-copyright=

en-aut-name=IbiTakayuki
en-aut-sei=Ibi
en-aut-mei=Takayuki
kn-aut-name=揖斐隆之
kn-aut-sei=揖斐
kn-aut-mei=隆之
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=動物遺伝育種分野
en-keyword=Marbling score
kn-keyword=Marbling score
en-keyword=Marbling on top round
kn-keyword=Marbling on top round
en-keyword=genetic parameter
kn-keyword=genetic parameter
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=53
end-page=59
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Mekong - Sight of Agriculture and Food -
kn-title=メコン　その農と食
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author has had the opportunity to be engaged in field work in the Mekong River basin for forty years. In this report, the variety of agriculture and food of the Mekong River basin are described, based on that experience. The Mekong River is the biggest river in Southeast Asia. The source of the Mekong River is Tibet. The river flows through Chinese Yunnan, through Myanmar, Laos, Thailand, and Cambodia, forming a delta in Vietnam where it comes out into the sea. There live many people in that basin, and various agricultural elements of cultural complexity are found. Food culture also varies throughout this area. The author divided the whole basin into seven agriculture zones. The key words indicating the various forms of agriculture and food cultures recognized in the Mekong River basin are listed, illustrated by photographs. It has been said that Chinese ancient civilization was started from the Yellow River civilization. The Chang Jiang civilization now comes to the fore, which started before the Yellow River civilization. It is just now being admitted that the Chang Jiang civilization is equal to the four big civilizations of the world. It is just now being recognized that the Chang Jiarg civilization is on a par with the four big civilization of the word. According to the author’s supposition, not only Chang Jiang but also Mekong, Thanlwin and Huang river constituted a Asian Big civilization. The author predicts whether a time will come when the existence of a “Asian Big River civilization” will come to be explained.
en-copyright=
kn-copyright=

en-aut-name=KurodaToshiro
en-aut-sei=Kuroda
en-aut-mei=Toshiro
kn-aut-name=黒田俊郎
kn-aut-sei=黒田
kn-aut-mei=俊郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=応用植物科学コース
en-keyword=Asian Big River civilization
kn-keyword=Asian Big River civilization
en-keyword=Chang Jiang civilization
kn-keyword=Chang Jiang civilization
en-keyword=Chinese ancient civilization
kn-keyword=Chinese ancient civilization
en-keyword=Mekong
kn-keyword=Mekong
en-keyword=Southeast Asia
kn-keyword=Southeast Asia
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=39
end-page=51
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=エジプト紅海沿岸のマングローブ林の林分構造
kn-title=Forest structure of gray mangrove (Avicennia marina) along Egyptian Red Sea coast
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Established mangrove forests along the coastal area of the Arabian Peninsula and African side of the Red Sea are uniquely different from mangrove forests in other parts of the world because of their low biodiversity and harsh habitat of arid and highly saline conditions. Therefore mangrove forests in this area appear in patchy and scattered patterns at mouths of wadi or in sheltered lagoons with rare and irregular flooding. Most of them are pure forests of Avicennia marina, occasionally mixed with Rhizophora mucronata in the southern part of the Red Sea. In this study, we analyze the forest structure of A. marina and discuss the regeneration strategy and the forest dynamics of this unique mangrove species. Three experimental plots of 1000 to 2000 trees/ha were selected from north to south along the Red Sea coast. The highest tree size (6.8m) suggested severe effects of the high salinity of the Red Sea (3.2 to 4.9%) on tree growth. Dense mantle vegetation had developed at the forest edge facing the open sea to protect the forest interior against strong waves and wind. Tree growth was also prevented by severe drought on the landside edge of the forest. All the forests had a dense seedling bank throughout the forest floor, with a very high rate of turnover and regeneration, which seldom occurred in other forests.
en-copyright=
kn-copyright=

en-aut-name=YoshikawaKen
en-aut-sei=Yoshikawa
en-aut-mei=Ken
kn-aut-name=吉川賢
kn-aut-sei=吉川
kn-aut-mei=賢
aut-affil-num=1
ORCID=

en-aut-name=InoueMasaki
en-aut-sei=Inoue
en-aut-mei=Masaki
kn-aut-name=井上正樹
kn-aut-sei=井上
kn-aut-mei=正樹
aut-affil-num=2
ORCID=

en-aut-name=YoshimoriIchidou
en-aut-sei=Yoshimori
en-aut-mei=Ichidou
kn-aut-name=吉森一道
kn-aut-sei=吉森
kn-aut-mei=一道
aut-affil-num=3
ORCID=

en-aut-name=NakashimaAtsushi
en-aut-sei=Nakashima
en-aut-mei=Atsushi
kn-aut-name=中島敦司
kn-aut-sei=中島
kn-aut-mei=敦司
aut-affil-num=4
ORCID=

en-aut-name=TeraminamiTomohiro
en-aut-sei=Teraminami
en-aut-mei=Tomohiro
kn-aut-name=寺南智弘
kn-aut-sei=寺南
kn-aut-mei=智弘
aut-affil-num=5
ORCID=

en-aut-name=MatsuoNaoko
en-aut-sei=Matsuo
en-aut-mei=Naoko
kn-aut-name=松尾奈緒子
kn-aut-sei=松尾
kn-aut-mei=奈緒子
aut-affil-num=6
ORCID=

en-aut-name=BanjoRyo
en-aut-sei=Banjo
en-aut-mei=Ryo
kn-aut-name=萬城遼
kn-aut-sei=萬城
kn-aut-mei=遼
aut-affil-num=7
ORCID=

en-aut-name=MiyamotoChiharu
en-aut-sei=Miyamoto
en-aut-mei=Chiharu
kn-aut-name=宮本千晴
kn-aut-sei=宮本
kn-aut-mei=千晴
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University

affil-num=2
en-affil=
kn-affil=Faculty of Agriculture, Okayama University

affil-num=3
en-affil=
kn-affil=Faculty of Agriculture, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Systems Engineering, Wakayama University

affil-num=5
en-affil=
kn-affil=Graduate School of Systems Engineering, Wakayama University

affil-num=6
en-affil=
kn-affil=Graduate School of Bioresources, Mie University

affil-num=7
en-affil=
kn-affil=Faculty of Bioresources, Mie University

affil-num=8
en-affil=
kn-affil=NGO, Action for Mangrove Reforestation
en-keyword=Gray mangrove (Avicennia marina)
kn-keyword=Gray mangrove (Avicennia marina)
en-keyword=the Red Sea
kn-keyword=the Red Sea
en-keyword=forest structure
kn-keyword=forest structure
en-keyword=seedling bank
kn-keyword=seedling bank
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=31
end-page=37
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of seed lots and seed production time on growth, flowering and cut flower quality in Bupleurum rotundifolium L.
kn-title=スプレーカーネーションの灌水同時施肥栽培における 栽植方式が収量および切り花品質に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effects of three planting patterns; two-row-planting high density (25 plants·m(-2)), two-row-planting low density (12.5 plants·m(-2)), and four-row-planting (25 plants·m(-2)) on the cut flower number and quality of spray carnation (Dianthus caryophyllus L.) cv. ‘Barbara’ and ‘Cherry Tessino’ grown by fertigation cultivation were investigated. In ‘Barbara’, there was no difference in total cut flower number between two-row-planting high density and four-row-planting. Total cut flower number of two-row-planting low density decreased to 60% of two-row-planting high density or four-row-planting. In ‘Cherry Tessino’, the total cut flower number of two-row-planting high density was 15% more than that of four-row-planting. The total cut flower number of two-row-planting low density decreased to 60~70% of two-row-planting high density or four-row-planting. There was no difference in cut flower quality between two-row-planting high density and four-row-planting in both cultivars. Cut flower weight and stem diameter of two-row-planting low density increased compared to those of two-row-planting high density or four-row-planting. Thus, the result indicates that two-row-planting high density may be suitable for fertigation cultivation in carnation.
en-copyright=
kn-copyright=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=山中正仁
kn-aut-sei=山中
kn-aut-mei=正仁
aut-affil-num=1
ORCID=

en-aut-name=IshiiMayumi
en-aut-sei=Ishii
en-aut-mei=Mayumi
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=2
ORCID=

en-aut-name=FujiwaraKazuki
en-aut-sei=Fujiwara
en-aut-mei=Kazuki
kn-aut-name=東浦優
kn-aut-sei=東浦
kn-aut-mei=優
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=大学院自然科学研究科

affil-num=2
en-affil=
kn-affil=大学院自然科学研究科

affil-num=3
en-affil=
kn-affil=兵庫県立農林水産技術総合センター
en-keyword=cultivated by fertigation
kn-keyword=cultivated by fertigation
en-keyword=planting density
kn-keyword=planting density
en-keyword=planting pattern
kn-keyword=planting pattern
en-keyword=spray carnation
kn-keyword=spray carnation
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=25
end-page=29
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of seed lots and seed production time on growth, flowering and cut flower quality in Bupleurum rotundifolium L.
kn-title=種子のロットおよび採種時期がブプレウルムの 生育，開花および切り花形質に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effects of seed lots (No. 021793, 025090, 026247, 027668) and seed production time on growth, flowering and cut flower quality in Bupleurum rotundifolium L. were investigated. The rate of germination was different depending on the seed lots, 021793 was lowest, and 026247 was highest. Days to visible bud and flowering were the shortest at 025090, and longest at 026247. The rate of germination of seed harvested on home seed-raising was different for seed production time. Seed harvested during high temperature
period from the middle of June to the beginning of July showed remarkably high rate of germination.
While plants grown by seed harvested during high temperature period remarkably delayed flowering and had low cut flower quality, plants grown on seed harvested during low temperature period enhanced flowering and had high cut flower quality. There was a little difference in parental line. It was thought that seed production time was affected by the environmental conditions during seed production time than by the seed lot.
en-copyright=
kn-copyright=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=1
ORCID=

en-aut-name=IshiiMayumi
en-aut-sei=Ishii
en-aut-mei=Mayumi
kn-aut-name=石井真由美
kn-aut-sei=石井
kn-aut-mei=真由美
aut-affil-num=2
ORCID=

en-aut-name=FujiwaraKazuki
en-aut-sei=Fujiwara
en-aut-mei=Kazuki
kn-aut-name=藤原一毅
kn-aut-sei=藤原
kn-aut-mei=一毅
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=
kn-affil=岡山大学大学院自然科学研究科
en-keyword=cut flower quality
kn-keyword=cut flower quality
en-keyword=environmental condition
kn-keyword=environmental condition
en-keyword=home seed-raising
kn-keyword=home seed-raising
en-keyword=parental line
kn-keyword=parental line
en-keyword=water absorption
kn-keyword=water absorption
en-keyword=seed production time
kn-keyword=seed production time
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=17
end-page=23
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Growth and Yield of Substrate-Cultured Strawberry as Affected by Root Zone Volume and Fertigation Frequency
kn-title=ピート栽培における培地量と給液制御方法がイチゴの生育と 収量に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effects of root-zone volume and fertigation frequency on 4 cultivars of substrate-grown strawberry (Fragaria×ananassa Duch., ‘Nyoho’, ‘Asukarubi’, ‘Akihime’ and ‘Tochiotome’) were investigated. Fertigation frequency had no significant effect on growth and yield of strawberry even in the minimum root-zone volume of 0.6L/plant. Total yield, mean berry weight, and root dry weight at end of harvest decreased with decrease in volume, but no significant difference was observed in number of harvested berries and subsequent growth of runners and daughter plants. In the yield and root growth, there were large differences between peat bags (2.25L/plant) and bowl-shaped containers (0.6-1.5L/plant), and highly significant non-linear regressions between the two parameters and the root-zone volume. The differences may be caused by factors other than the volume, such as the shape of root-zone and edge of containers or drain property, and so on.
en-copyright=
kn-copyright=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=1
ORCID=

en-aut-name=HiroseYasuhisa
en-aut-sei=Hirose
en-aut-mei=Yasuhisa
kn-aut-name=廣瀬泰久
kn-aut-sei=廣瀬
kn-aut-mei=泰久
aut-affil-num=2
ORCID=

en-aut-name=MorimotoYukari
en-aut-sei=Morimoto
en-aut-mei=Yukari
kn-aut-name=森本由香里
kn-aut-sei=森本
kn-aut-mei=由香里
aut-affil-num=3
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=応用植物科学コース

affil-num=2
en-affil=
kn-affil=応用植物科学コース

affil-num=3
en-affil=
kn-affil=応用植物科学コース

affil-num=4
en-affil=
kn-affil=応用植物科学コース
en-keyword=Fragaria×ananassa
kn-keyword=Fragaria×ananassa
en-keyword=root
kn-keyword=root
en-keyword=runner
kn-keyword=runner
en-keyword=solar radiation
kn-keyword=solar radiation
en-keyword=water absorption
kn-keyword=water absorption
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=9
end-page=15
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Application of Controlled-release Fertilizer to Netted Melon Cultivation Using Root-proof Capillary Wick
kn-title=ネットメロンの防根給水ひも栽培における肥効調節型肥料の適用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Use of controlled-release fertilizer in limited amount of soil and sand medium for netted melon cultivation using a root-proof capillary wick was investigated. The first experiment consisted of 3 lots of soil medium. 1) 3L medium used throughout the cultivation period (A); 2) 6L medium used throughout the cultivation period (B); 3) 3L used until pollination stage, then a partitioning board removed to increase the growth medium to 6L (C). Average of one fruit weight was 1.4 kg in A and B, but only 1.2 kg in C. Brix° value was 13.1 in A, 13.6 in B and 13.4 in C, respectively. The stem diameter at the time of pollination was largest in B in which there was fruit cracking that was also observed in C, but not in A. The root dry weight was highest in A. These results indicate that the restricted medium of 3L presents a potential for good fruit production. The second experiment consisted of 4 lots with 3L medium each: 1) soil and fertilizer medium as in experiment 1; 2) sand and fertilizer medium as in lot 1); 3) sand and slower release fertilizer; 4) sand and half of fertilizer at planting and an additional fertilizer bag placed on the wick at time of pollination. Although cracking fruits occurred 75% in 3) and 37.5% in 4), there was no cracking of fruits in 1) and 2), in which fruit weight in the latter was 1.5kg and brix° value of 14.5 was obtained. These results indicate that controlled-release fertilizer is useful for fruit production
of netted melon in sand medium of 3L with a high potential for reuse.
en-copyright=
kn-copyright=

en-aut-name=KawaharaMasaki
en-aut-sei=Kawahara
en-aut-mei=Masaki
kn-aut-name=川原雅規
kn-aut-sei=川原
kn-aut-mei=雅規
aut-affil-num=1
ORCID=

en-aut-name=MurakamiSayo
en-aut-sei=Murakami
en-aut-mei=Sayo
kn-aut-name=村上紗代
kn-aut-sei=村上
kn-aut-mei=紗代
aut-affil-num=2
ORCID=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=応用植物科学コース

affil-num=2
en-affil=
kn-affil=応用植物科学コース

affil-num=3
en-affil=
kn-affil=応用植物科学コース
en-keyword=medium volume
kn-keyword=medium volume
en-keyword=sand medium
kn-keyword=sand medium
en-keyword=fruit yield
kn-keyword=fruit yield
en-keyword=cracking fruit
kn-keyword=cracking fruit
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=3
end-page=7
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Purification and Characterization of l-Methionine Decarboxylase from Streptomyces sp. 590
kn-title=放線菌Streptomyces sp.590由来l-メチオニン脱炭酸酵素の精製および性質検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=L-Methionine decarboxylase [EC 4.1.1.57] catalyzes the decarboxylation of L-methionine and is a pyridoxal 5’-phosohate(PLP)-dependent enzyme. L-Methionine decarboxylase has been purified 630-fold by DEAE-Toyopearl 650M, Phenyl-Toyopearl 650M and Sephacryl S-300 column chromatographies from Streptomyces sp.590. The enzyme has a dimeric structure with identical subunits of Mr 60,000. This enzyme shows optimum activity at pH7.0 and 45°C, and is stable between pH5.7 and pH9.0. L-Methionine decarboxylase has antitumor activity against RERF-LC-AI and HeLa cells. Ten N-terminal amino acid sequence of L-methionine decarboxylase was determined, and the sequence showed no homology with other reported proteins.
en-copyright=
kn-copyright=

en-aut-name=MaemuraTomomi
en-aut-sei=Maemura
en-aut-mei=Tomomi
kn-aut-name=前村知美
kn-aut-sei=前村
kn-aut-mei=知美
aut-affil-num=1
ORCID=

en-aut-name=UchitomiKumiko
en-aut-sei=Uchitomi
en-aut-mei=Kumiko
kn-aut-name=内富久美子
kn-aut-sei=内富
kn-aut-mei=久美子
aut-affil-num=2
ORCID=

en-aut-name=KusakaChika
en-aut-sei=Kusaka
en-aut-mei=Chika
kn-aut-name=日下知香
kn-aut-sei=日下
kn-aut-mei=知香
aut-affil-num=3
ORCID=

en-aut-name=InagakiJunko
en-aut-sei=Inagaki
en-aut-mei=Junko
kn-aut-name=稲垣純子
kn-aut-sei=稲垣
kn-aut-mei=純子
aut-affil-num=4
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=5
ORCID=

en-aut-name=SodaKenji
en-aut-sei=Soda
en-aut-mei=Kenji
kn-aut-name=左右田健次
kn-aut-sei=左右田
kn-aut-mei=健次
aut-affil-num=6
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=農芸化学コース

affil-num=2
en-affil=
kn-affil=農芸化学コース

affil-num=3
en-affil=
kn-affil=農芸化学コース

affil-num=4
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科

affil-num=5
en-affil=
kn-affil=農芸化学コース

affil-num=6
en-affil=
kn-affil=京都大学

affil-num=7
en-affil=
kn-affil=農芸化学コース
en-keyword=L-methionine decarboxylase
kn-keyword=L-methionine decarboxylase
en-keyword=pyridoxal 5’-phosohate
kn-keyword=pyridoxal 5’-phosohate
en-keyword=Streptomyces
kn-keyword=Streptomyces
en-keyword=decarboxylation of L-methionine
kn-keyword=decarboxylation of L-methionine
END

start-ver=1.4
cd-journal=joma
no-vol=100
cd-vols=
no-issue=
article-no=
start-page=1
end-page=2
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=岡山大学農学部学術報告100巻の発刊に際して
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=神崎浩
kn-aut-sei=神崎
kn-aut-mei=浩
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学農学部
END

start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=1
article-no=
start-page=46
end-page=51
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=1953
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ゴボウモグリバエ Melanagromvza lappivora sp. n. について（双翅目農園芸害虫の分類及び生活史の研究 第3報）
kn-title=Melanagromyza lappivora, a new speCIes of root miner of Burdock in Japan (Diptera, Agromyzidae). (Taxonomy and bionomics of Dipterous pests for farm and gardenlin Japan. III)
en-subtitle=
kn-subtitle=
en-abstract=近年，岐阜地方で問題となつた，ゴボウの根に潜入加害するゴボウネモグリバエに関し，岐阜農試より送附された標本にもとづき，これの分類上の位置並びに形態を明にし，次の新学名を与へた．Melanagromyza lappivora Koizumi
kn-abstract=Recently, Mr. Muto of the Gifu Agric. Exp. Sta. observed that the edible roots of Burdock "Gobo" (Arctium lappa L.) cultivated in Gifu district were severely injured by the larvae of an Agromyzid fly. This is apparently a new pest of Burdock and its bionomics and control measures are being studied by him. After examination of the specimens which recieved from Mr. Muto, I came to the conclusion that this Agromyzid is undescribed form. The present paper deals with the taxonomical study of this fly. The terminology used here are taken from Dr. Frick (1952) with minor exceptions. I wish to express here my sincere thanks to Mr. Toshiro Muto of the Gifu Agricultural Experiment Station for the gift of the specimens, and also to Prof. Dr. Chukichi Harukawa of the Okayama University for his kindness in reading through this manuscript.
en-copyright=
kn-copyright=

en-aut-name=KoizumiKenji
en-aut-sei=Koizumi
en-aut-mei=Kenji
kn-aut-name=小泉憲治
kn-aut-sei=小泉
kn-aut-mei=憲治
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=1
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1953
dt-pub=1953
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=SCIENTIFIC REPORTS No.3 ERRATA
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=97
end-page=113
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Papers Published by Members of the Faculty from January to December 2009.
kn-title=公表学術論文等リスト 2009
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=85
end-page=95
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Analysis and application of meat quality characteristics
kn-title=食肉の品質特性の解析と応用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The quality of food has always been evaluated by the five senses of human. It is possible for a sensory evaluation with no special device for anyone anywhere anytime. The evaluation is only result information, but it is not possible by evaluation to obtain cause information for quality control. Among evaluation information by the five senses for the food, sight has been developed most as the scientific method. The next development is texture, and then taste, odor and hearing are just developing. Deterioration of food becomes economic loss then results in environment load, so quality control is important. Instrumental analyses of the qualities of taste and flavor have not been very developed. Actually, the sensory evaluations for sight, odor, taste, hearing and texture examination are performed by the senses, but it is only evaluation. The evaluation is results information and there is a limit to know cause information. Sensory evaluation is subjective, vague and may become arbitrary. Quality control is difficult by non experts with knowledge and experience. Therefore, cause information for scientific quality control was integrated with result information as evaluation. Objective cause information for the quality control is obtained by computer connected with the instrument having sensory sensor. In this report, first, differences of evaluation and quality control for meat quality are explained. Successively, automatic analysis of characteristics of color quality and quality control, human interface of texture quality, inhibition of cells DNA damage by heme protein, the method of color development without nitrous acid and inhibition with fig protease against ACE, angiotensin-I converting enzyme were described.
en-copyright=
kn-copyright=

en-aut-name=IzumimotoMasatoshi
en-aut-sei=Izumimoto
en-aut-mei=Masatoshi
kn-aut-name=泉本勝利
kn-aut-sei=泉本
kn-aut-mei=勝利
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=meat
kn-keyword=meat
en-keyword=computer analysis
kn-keyword=computer analysis
en-keyword=evaluation
kn-keyword=evaluation
en-keyword=quality control
kn-keyword=quality control
en-keyword=color
kn-keyword=color
en-keyword=texture
kn-keyword=texture
en-keyword=DNA damage
kn-keyword=DNA damage
en-keyword=fig protease
kn-keyword=fig protease
en-keyword=ACE
kn-keyword=ACE
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=71
end-page=84
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=リン脂質過酸化物の生物有機化学的合成
kn-title=Chemoenzymatic Synthesis of Phospholipid Hydroperoxides
en-subtitle=
kn-subtitle=
en-abstract=健康正常人の血液などの組織中にはリン脂質過酸化物が極微量で存在し，疾病や老化によってその濃度が顕著に上昇する事が知られている．その事が明らかにされた当初は，極めて複雑な混合物をなす生体脂質中に含まれる不安定な極微量の過酸化脂質を単離・構造決定する事は殆ど不可能と考えられていた．現在もなお，そのような脂質過酸化物を生体組織から純粋に取り出し，構造決定したという報告は無い．従ってそのような分子種の化学的・生理学的性質は不明であったが，脂肪酸過酸化物が毒性を示す事から，リン脂質過酸化物もおそらく毒性を示すだろうと考えられてきた．このような漠然とした推定を科学的に明らかにするためには，化学合成によらざるを得ない．我々はこの未知の合成に取りかかった．しかし，従来の化学的手法のみでは不可能である事も明らかであった．その中で予想された困難の一つは極めて不安定なヒドロペルオキシ基を不飽和脂肪酸のある特定の位置にどのように導入するかという問題と，ヒドロペルオキシ基に影響を与える事なく合成中間体をどのように化学変換するかであった．第一の問題に対する解決策として，不飽和脂肪酸に大豆リポキシゲナーゼを作用させる事で解決する事ができた．植物に広く分布する酵素であるリポキシゲナーゼは植物中でリノール酸に作用して過酸化し，その生成物にヒドロペルオキシドリアーゼという酵素が作用して種々のアルデヒドが精製し，これは植物の青臭みを与える．第二の問題に対しては，リノール酸に導入された不安定なヒドロペルオキシ基をパーアセタールとしての保護する事により解決した．この保護基は，中間体から最終生成物に至るまでの反応条件，例えばDCCによるアシル化反応に対して安定である事が明らかとなった．この二つの問題を解決する事によって，リン脂質過酸化物の一つであるホスファチジルコリン過酸化物を世界に先駆けて成功した．さらにこのホスファチジルコリン過酸化物に微生物由来のホスフォリパーゼDを作用させる事によってホスファチジルエタノールアミン過酸化物，ホスファチジルセリン過酸化物やホスファチジルグリセロール過酸化物の合成にも成功した．また，トリグリセリド過酸化物の合成も可能になった．これらの脂質過酸化物が化学的に実態のあるものとして認識されてから，その生理作用に関する研究が広範に行われている．しかし，生体組織に存在するリン脂質過酸化物の生理学的役割は依然として明らかになっていない．ある種のリン脂質過酸化物が動物の免疫系を活性化するという報告もあり，必ずしも生体に対して悪い作用をするばかりではないようである．
kn-abstract=Chemoenzymatic synthesis of 1-stearoyl-2-hydropeoxyacyl-sn-glycerophospholipids including phosphatidylcholine (PC-OOH), phosphatidic acid (PA-OOH), phosphatidylethanolamine (PE-OOH), phosphatidylglycerol (PG-OOH) and phosphatidylserine (PS-OOH). The hydroperoxy acyl moieties were prepared via hydroperoxidation of linoleic, dihomo-γ-linolenic and arachidonic acids by soybean, potate lipoxygenase or autoxidation. Their hydroperoxy group was protected as a dimethylperacetal before condensation with lysophosphatidylcholine. Optically active lysophosphatidylcholine was prepared via short pathway involving lipase-catalyzed direct enantioselective stearoylation of 2-O-benzylglycerol and choline phosphate synthesis. Peroxy fatty acids and lysophosphatidylcholine thus obtained were condensed using dicyclohexylcarbodiimide
in chloroform. Removing the peracetal group in the product and purification by reverse-phase chromatography afforded the desired PC-OOH’s. PA-OOH, PG-OOH, PE-OOH and PS-OOH were obtained by phospholipase-D catalyzed transphosphatidylation from PC-OOH. As a reference compound for biological studies of hydroperoxy phopholipid, PC-OH's were also prepared in which hydroxy unsaturated fatty acyl group was linked to the sn-2 position of the glycerophospholipids.
en-copyright=
kn-copyright=

en-aut-name=BabaNaomichi
en-aut-sei=Baba
en-aut-mei=Naomichi
kn-aut-name=馬場直道
kn-aut-sei=馬場
kn-aut-mei=直道
aut-affil-num=1
ORCID=

en-aut-name=YonedaKenji
en-aut-sei=Yoneda
en-aut-mei=Kenji
kn-aut-name=米田健司
kn-aut-sei=米田
kn-aut-mei=健司
aut-affil-num=2
ORCID=

en-aut-name=SasakuraKeiji
en-aut-sei=Sasakura
en-aut-mei=Keiji
kn-aut-name=笹倉敬司
kn-aut-sei=笹倉
kn-aut-mei=敬司
aut-affil-num=3
ORCID=

en-aut-name=ShigetaYasutami
en-aut-sei=Shigeta
en-aut-mei=Yasutami
kn-aut-name=繁田泰民
kn-aut-sei=繁田
kn-aut-mei=泰民
aut-affil-num=4
ORCID=

en-aut-name=KishidaYasuhiro
en-aut-sei=Kishida
en-aut-mei=Yasuhiro
kn-aut-name=岸田靖弘
kn-aut-sei=岸田
kn-aut-mei=靖弘
aut-affil-num=5
ORCID=

en-aut-name=AoishiAkihiro
en-aut-sei=Aoishi
en-aut-mei=Akihiro
kn-aut-name=青石晃宏
kn-aut-sei=青石
kn-aut-mei=晃宏
aut-affil-num=6
ORCID=

en-aut-name=DaidoHiroko
en-aut-sei=Daido
en-aut-mei=Hiroko
kn-aut-name=大同浩子
kn-aut-sei=大同
kn-aut-mei=浩子
aut-affil-num=7
ORCID=

en-aut-name=NakajimaShuhei
en-aut-sei=Nakajima
en-aut-mei=Shuhei
kn-aut-name=中島修平
kn-aut-sei=中島
kn-aut-mei=修平
aut-affil-num=8
ORCID=

en-aut-name=IwasaJunkichi
en-aut-sei=Iwasa
en-aut-mei=Junkichi
kn-aut-name=岩佐順吉
kn-aut-sei=岩佐
kn-aut-mei=順吉
aut-affil-num=9
ORCID=

en-aut-name=TaharaShoichi
en-aut-sei=Tahara
en-aut-mei=Shoichi
kn-aut-name=田原正一
kn-aut-sei=田原
kn-aut-mei=正一
aut-affil-num=10
ORCID=

en-aut-name=KanekoTakao
en-aut-sei=Kaneko
en-aut-mei=Takao
kn-aut-name=金子孝夫
kn-aut-sei=金子
kn-aut-mei=孝夫
aut-affil-num=11
ORCID=

en-aut-name=MatsuoMitsuyoshi
en-aut-sei=Matsuo
en-aut-mei=Mitsuyoshi
kn-aut-name=松尾光芳
kn-aut-sei=松尾
kn-aut-mei=光芳
aut-affil-num=12
ORCID=

en-aut-name=ShimizuSakayu
en-aut-sei=Shimizu
en-aut-mei=Sakayu
kn-aut-name=清水昌
kn-aut-sei=清水
kn-aut-mei=昌
aut-affil-num=13
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岡山大学

affil-num=6
en-affil=
kn-affil=岡山大学

affil-num=7
en-affil=
kn-affil=岡山大学

affil-num=8
en-affil=
kn-affil=岡山大学

affil-num=9
en-affil=
kn-affil=岡山大学

affil-num=10
en-affil=
kn-affil=東京都老人総合研究所

affil-num=11
en-affil=
kn-affil=東京都老人総合研究所

affil-num=12
en-affil=
kn-affil=東京都老人総合研究所

affil-num=13
en-affil=
kn-affil=京都大学農学部
en-keyword=phospholipid
kn-keyword=phospholipid
en-keyword=peroxide
kn-keyword=peroxide
en-keyword=hydroperoxide
kn-keyword=hydroperoxide
en-keyword=phospholipase D
kn-keyword=phospholipase D
en-keyword=lipoxygenase
kn-keyword=lipoxygenase
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=63
end-page=70
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Saline Irrigation using Ion-exchange Resin and Reverse Osmosis Membrane in Wheat and Barley
kn-title=イオン交換樹脂および逆浸透膜を用いたコムギとオオムギの塩水灌漑栽培
en-subtitle=
kn-subtitle=
en-abstract=塩水を農業用水として利用し淡水資源を節約するために，根域に除塩処理を施す塩水灌漑法を検討した．内径56㎜，長さ40㎝の塩ビ製のポットを用いた．ポットの底部には除塩効果があるアンバーライト（イオン交換樹脂）および逆浸透膜を設置する区と除塩効果がないラブシート（不織布）を設置する対照区を設けた．そしてポットに土壌を詰めコムギとオオムギを移植し，ポットの底から淡水および塩水で灌漑した．脱塩処理および灌漑に対する反応は２作物で同じであった．対照区では塩水灌漑によって生育が著しく抑制された．アンバーライト区で作物の生育は，淡水灌漑で対照区と変わらなかった．塩水灌漑ではアンバーライトによって乾物生産が対照区より向上したが，最終的な茎葉部と土壌のナトリウム含有率は対照区と変わらなかった．逆浸透膜は茎葉部と土壌のナトリウム濃度を低下させた．しかし逆浸透膜によって作物の生育は，淡水および塩水の二つの灌漑条件で対照区より著しく小さくなった．アンバーライトと逆浸透膜はともに除塩効果があった．しかし，アンバーライトは塩水処理能力の限界があること，逆浸透膜は水の移動が阻害されることが解決しなければならない課題であることが分かった．
kn-abstract=Saline irrigation equipped desaline apparatus in a root zone was tested to save fresh water resources. Pots of 56 mm inner-diameter and 40 cm in length were used. Amberlite (ion-exchange resin) and reverse osmosis membrane, both of which had a desalinating ability, were set at the bottom of each pot in desaline plots, respectively. In the control plot, a lovesheet (non-woven cloth) that had no ability to desaline was set. Wheat and barley were transplanted into these pots, which were filled with soil, and the pots were irrigated through the bottom either by fresh or saline water. The two crops responded to desaline methods and irrigation similarly. Growth and development in the two crops were suppressed largely by saline irrigation in the control plot. Amberlite did not affect the growth and development under fresh water irrigation. Shoot dry weight was increased by amberlite under saline irrigation, but the final values of sodium concentration of shoot and soil was not affected. The sodium concentration was reduced by reverse osmosis membrane, whereas the growth and development were suppressed under both fresh and saline irrigations. Amberlite and reverse osmosis membrane improved the growth and development and sodium concentration, but it should be a challenge to improve desaline ability of amberlite and water permeability of reverse osmosis membrane.
en-copyright=
kn-copyright=

en-aut-name=TsudaMakoto
en-aut-sei=Tsuda
en-aut-mei=Makoto
kn-aut-name=津田誠
kn-aut-sei=津田
kn-aut-mei=誠
aut-affil-num=1
ORCID=

en-aut-name=YokomizoYuka
en-aut-sei=Yokomizo
en-aut-mei=Yuka
kn-aut-name=横溝友香
kn-aut-sei=横溝
kn-aut-mei=友香
aut-affil-num=2
ORCID=

en-aut-name=TakamiShinichi
en-aut-sei=Takami
en-aut-mei=Shinichi
kn-aut-name=高見晋一
kn-aut-sei=高見
kn-aut-mei=晋一
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=近畿大学農学部
en-keyword=Amberlite
kn-keyword=Amberlite
en-keyword=Desaline method
kn-keyword=Desaline method
en-keyword=Reverse osmosis membrane
kn-keyword=Reverse osmosis membrane
en-keyword=Saline irrigation
kn-keyword=Saline irrigation
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=55
end-page=62
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Nutrient Solution Concentration on Cut Flower Yield, Quality and Characteristics of Seasonal Nutrient Absorption of Spray Carnation Grown by Cultivation in Drip Fertigation
kn-title=給液濃度がかん水同時施肥法で栽培したスプレーカーネーションの切り花収量と品質および時期別養分吸収特性に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=スプレーカーネーションのかん水同時施肥法における養分吸収特性を‘バーバラ’で調査した．窒素：リン（P）：カリウム（K）：カルシウム（Ca）：マグネシウム（Mg）の含有率（%）が15：6.5：12.5：3.6：0.9の液肥を，低濃度（3,000倍），中濃度（1,500倍），高濃度（1,000倍）の3水準で，毎日0.0～13.9㎜ずつ点滴給液した．切り花収量は中濃度区と高濃度区が多く，切り花品質は2番花において低濃度区の切り花重が減少した．植物体の窒素およびカリウム含有率は乾物増加量が増大するにつれて低下し，リンは秋期と春期に減少した．低濃度区の窒素，カリウムの含有率は土壌養分含有量の減少にともない，低下した．高濃度区では土壌の窒素およびリン含有量が栽培終了時に大幅に増大した．中濃度での給液における１株当たりの総養分吸収量は窒素が2,807.0㎎，リンが493.5㎎，カリウムが3,667.4㎎，カルシウムが1,615.8㎎，マグネシウムが320.9㎎であった．リン，カリウム，カルシウム，マグネシウムの窒素に対する吸収割合はいずれも春期に約1.3倍に上昇した．スタンダードカーネーションとの比較では，スプレーカーネーションでは，ややリンの植物体含有率と吸収量が少ないが，窒素，カリウム，カルシウム，マグネシウムの植物体含有率および吸収量には，ほとんど差がなかった．
kn-abstract=Effects of nutrient solution concentration on cut flower yield, quality and characteristics of seasonal nutrient absorption of spray carnation ‘Barbara’ grown by cultivation in drip fertigation were investigated.
'Barbara' was grown with a nutrient solution (N:15%, P:6.5%, K:12.5%, Ca:3.6%, Mg:0.9%), at three concentration levels which were high concentration (1,000 times), middle concentration (1,500 times) and low concentration (3,000 times). The daily amount of nutrient solution was ranged from 0.0mm to 13.9mm. The total cut flower yield of middle concentration and high concentration were bigger than that of low concentration. The weight of the second cut flower of low concentration was decreased as compared to that of middle concentration and high concentration. Nitrogen (N) and potassium (K) in the plant nutrient ratio were decreased as the increase of dry matter and phosphorus
(P) was increased at autumn and spring. With a reduction in the soil nutrient contents, Nitrogen and potassium in the plant nutrient ratio of low concentration were decreased. Nitrogen and phosphorus in the soil nutrient contents of high concentration were increased at the end of cultivation. In the total nutrient absorbed amount of middle concentration, 2,807.9 mg nitrogen, 493.5 mg phosphorus, 3,667.4 mg potassium, 1,615.8 mg calcium (Ca) and 320.9 mg magnesium (Mg) were absorbed per plant. In spring, the ratio of nitrogen absorbed amount to nutrients absorbed amount was increased 1.3 times. Phosphorus ratio and phosphorus absorbed amount in spray carnation were fewer than that of standard carnation. There were no differences in total nitrogen, potassium, calcium and magnesium absorption between spray carnation and standard carnation.
en-copyright=
kn-copyright=

en-aut-name=YamanakaMasahito
en-aut-sei=Yamanaka
en-aut-mei=Masahito
kn-aut-name=山中正仁
kn-aut-sei=山中
kn-aut-mei=正仁
aut-affil-num=1
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=2
ORCID=

en-aut-name=OgawaKinoe
en-aut-sei=Ogawa
en-aut-mei=Kinoe
kn-aut-name=小河甲
kn-aut-sei=小河
kn-aut-mei=甲
aut-affil-num=3
ORCID=

en-aut-name=UdaAkira
en-aut-sei=Uda
en-aut-mei=Akira
kn-aut-name=宇田明
kn-aut-sei=宇田
kn-aut-mei=明
aut-affil-num=4
ORCID=

en-aut-name=IwaiToyomichi
en-aut-sei=Iwai
en-aut-mei=Toyomichi
kn-aut-name=岩井豊通
kn-aut-sei=岩井
kn-aut-mei=豊通
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=兵庫県立農林水産技術総合センター

affil-num=4
en-affil=
kn-affil=兵庫県立農林水産技術総合センター

affil-num=5
en-affil=
kn-affil=兵庫県立農林水産技術総合センター
en-keyword=fertigation
kn-keyword=fertigation
en-keyword=nutrient absorption
kn-keyword=nutrient absorption
en-keyword=plant nutrient
kn-keyword=plant nutrient
en-keyword=soil nutrient
kn-keyword=soil nutrient
en-keyword=spray carnation
kn-keyword=spray carnation
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=49
end-page=53
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Flower Bud Differentiation and Flowering of Tray Grown Strawberry 'Nyoho' as Affected by Plant age and the Duration of Nutrient Starvation
kn-title=トレイ育苗したイチゴ‘女峰’の花芽分化と開花に及ぼす挿し苗時期と施肥中断時期の影響
en-subtitle=
kn-subtitle=
en-abstract=イチゴでは，根域容量130mL程度のセルトレイを利用した挿し苗育苗が省力育苗技術として急速に普及している．そこで，‘女峰’を用いて挿し苗時期と施肥中断時期が花芽分化と開花に及ぼす影響について検討した．挿し苗は7月中下旬が適期であり，6月や8月挿し苗区より花芽分化と開花が早く，極端に遅れる株が少なかった．液肥を用いて約6㎎-N/株・週の割合で与えた場合，施肥中断時期は8月末が最も優れ，週間早いと定植前の肥料不足によって極端に開花の遅れる株が増加し，1週間遅れると高窒素による花芽分化抑制が認められた．
kn-abstract=In the forcing production of strawberry Fragaria × ananassa Duch., the use of tray plants grown from cuttings of runner tips is becoming popular with the increase in strawberry production with substrate culture systems, due to their lower cost and ease of plant management compared to conventional pot-grown plants. Effects of the date of runner cutting and the duration of nutrient starvation on flower bud differentiation and flowering of tray grown plants of strawberry 'Nyoho' were investigated. Plants cut on mid or late July and rooted on cell trays performed early and uniform flower bud differentiation and flowering compared to June or August cut plants. When the plants were supplied nutrient solution containing ca. 6 mg-N/plant/week, nutrient starvation from the end of August led to the early and uniform flowering of tray plants, outperforming the earlier or later starvation.
en-copyright=
kn-copyright=

en-aut-name=YoshidaYuichi
en-aut-sei=Yoshida
en-aut-mei=Yuichi
kn-aut-name=吉田裕一
kn-aut-sei=吉田
kn-aut-mei=裕一
aut-affil-num=1
ORCID=

en-aut-name=MorimotoYukari
en-aut-sei=Morimoto
en-aut-mei=Yukari
kn-aut-name=森本由香里
kn-aut-sei=森本
kn-aut-mei=由香里
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=forcing
kn-keyword=forcing
en-keyword=Fragaria×ananassa Duch.
kn-keyword=Fragaria×ananassa Duch.
en-keyword=nutrient solution
kn-keyword=nutrient solution
en-keyword=runner cutting
kn-keyword=runner cutting
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=43
end-page=47
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Controlled-release Fertilizer and Root-proof Capillary Wick Addition to Root-zone on Tomato Growth and Yield
kn-title=トマトの防根給水ひも栽培における肥効調節型肥料と根域拡張に伴う「紐」の適用
en-subtitle=
kn-subtitle=
en-abstract=中玉トマト18段摘心の長期促成栽培における肥効調節型肥料の適用と生育途中の根域拡張に伴う「紐」適用が生育と果実収量に及ぼす効果を調べた．栽培容器は可動仕切り板で2つに仕切り区画当たり容量は2.8ℓとし，第8段花房開花時に仕切り板をはずして5.6ℓ（1st Box＋2nd Box）とした．試験区は2区で2nd Boxに紐を配置しない1本区と2nd Boxにもう１本を配した2本区である．2本区の草丈は1月以降，1本区に比べ高く推移し第18段花房の開花日が約10日早まり，成長の指標とした摘心時の茎頂部新鮮重も1本区の2倍となった．1株の果実収量は1本区で8kg，2本区で9kgとなり果実の糖度，酸度には両区間に差はなかった．栽培終了時の根の乾物重と茎切断面から採取した木部いっ泌液は両区間に差はなかったが，両区とも栽培終期の4月には生育が衰え葉色は黄緑色で果実の着色も不均一であった．木部いっ泌の成分分析ではNO(3)-N濃度が1本区で10me/ℓ，2本区で6me/ℓであり，これらの濃度は一般に養液栽培に使用される園試標準濃度よりも低かった．以上の結果から，第8段花房開花時に仕切りを開放し新区画への土壌と肥料ならびに紐の新たな適用は，生育安定と収量確保に効果的に作用することが明らかとなった．しかし，紐の有無にかかわらず生育後期の生育が不良となったことから，今後は施肥設計の面から，とくにNO(3)-NとSO(4)-Sの両面から検討する必要があると考察した．
kn-abstract=This study was conducted to investigate the effects of controlled-release fertilizers and wick addition to the root-zone on plant growth and yield of medium-fruit sized tomato up to the 18(th) truss in long-term forcing culture. The set-up consisted of a box partitioned by a board into two halves, named box 1 and box 2. Box 1 contained 2.8 ℓ of growth medium (soil : bark : perlite : peat=2 : 2 : 1 : 1). Plant was grown in box 1 with a capillary wick, and at flowering of the 8(th) truss, the partition was removed and box 2 filled with 2.8 ℓ of the medium. There were two treatments, with or without capillary wick in box 2, namely, single wick (S) and double wicks (D). Plant height was greater in D than S in January, and the difference increased gradually thereafter. Flowering time of 18(th) truss in D was 10 days earlier and decapitated shoot weight was twice that of S. Fruit yield per plant was 8 kg in S and 9 kg in D with similar value of Brix and titratable acidity. There was no difference between S and D in dry root weight (7g/plant) or in xylem exudates (8ml/h), while in S in April plant growth was inferior, leaf color yellow greenish and fruit colour uneven towards the end of growth of plants. Xylem sap analysis showed that NO(3)-N was 10 me/ℓ in S and 6 me/ℓ in D. This concentration is weaker than that of Enshi standard nutrient solution generally used in hydroponics. These results suggest that application of controlled-release fertilizers and wick addition to box 2 with root-zone extended was effective for plant growth performance and fruit yield.
en-copyright=
kn-copyright=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=1
ORCID=

en-aut-name=FujiiYuki
en-aut-sei=Fujii
en-aut-mei=Yuki
kn-aut-name=藤井由貴
kn-aut-sei=藤井
kn-aut-mei=由貴
aut-affil-num=2
ORCID=

en-aut-name=KinoshitaTakafumi
en-aut-sei=Kinoshita
en-aut-mei=Takafumi
kn-aut-name=木下貴文
kn-aut-sei=木下
kn-aut-mei=貴文
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=(独)農業・食品産業技術総合研究機構近畿中国四国農業研究センター
en-keyword=capillary watering
kn-keyword=capillary watering
en-keyword=combination of fertilizer
kn-keyword=combination of fertilizer
en-keyword=medium-fruit sized tomato
kn-keyword=medium-fruit sized tomato
en-keyword=sectional box
kn-keyword=sectional box
en-keyword=substrate volume
kn-keyword=substrate volume
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=35
end-page=42
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=休眠程度の異なるブドウ‘ピオーネ’の発芽に及ぼす温度の影響
kn-title=Effect of Temperature on 'Pione' Grapevine Budbreaking at Different Stages of Dormancy
en-subtitle=
kn-subtitle=
en-abstract=露地栽培されているブドウ‘ピオーネ’について，休眠の深さが異なる7月から翌年3月まで約1か月間隔で枝を採取し，1芽を有す挿し穂を調整した後，20，25および30℃に制御したインキュベーター（いずれも14時間日長）に入れ，経時的に発芽を調査した．発芽の早さを示す発芽所要日数と発芽の揃いを示す60%発芽所要日数から発芽に及ぼす温度の影響を評価した．実験期間中の温度を測定し，休眠完了と温度との関係を考察した．いずれの処理時期においても30℃の発芽が最も優れ，次いで25℃，20℃の順であった．しかし，発芽に及ぼす温度の影響は処理時期によって大きく異なった．すなわち，発芽所要日数は7月から10月までは徐々に増加し，11月に最大に達した後，3月に向けて少しずつ減少した．このことから，‘ピオーネ’では7月から9月が条件的休眠期，10月から12月が自発休眠期，1月から3月が他発休眠期と推察された．自発休眠期までの最終発芽率はいずれの温度も100%未満であり，また7月～9月の20℃処理では60%未満の発芽率であった．一方，自発休眠期の中期以降の処理ではいずれの温度とも均一な発芽を示し，最終発芽率はほぼ100%であった．11月以降の処理において，発芽所要日数と7.2℃以下の温度に遭遇した時間数（CCH）との間に有意な負の相関があった．また，11月１日から各処理時期までの0℃以上の温度に遭遇した時間数と20，25または30℃で処理を始めた日から各処理区の発芽までの時間数との積算（CT, ℃・h）との間にも有意な負の相関が認められた．以上のことから，‘ピオーネ’の芽の休眠完了の予測には低温遭遇量だけでなく，0℃以上の積算温度による方法も有効と考えられた．
kn-abstract=The effects of temperature on budbreak of cuttings obtained at different stages of dormancy from 'Pione' grapevines (Vitis labrusca × V. vinifera) grown in open field were investigated. Cuttings were collected at monthly intervals from July to March. Judging from the number of days to initial and 60% budbreak after treatment, indicating promotion and the uniformity of budbreak, respectively, 30℃ was the most effective in budbreak, followed by 25 and 20℃ in that order in all treatment times. However, the effect of temperature on budbreak was markedly affected by treatment time. The number of days to initial budbreak (NDIB) increased gradually from July to October, peaked in December and thereafter decreased gradually towards March. The periods from July to September, from October to December, and from January to March were assumed to correspond to paradormancy, endodormancy, and ecodormancy of 'Pione' grapevines, respectively. Final percentage of budbreak was less than 100% until endodormancy for all temperatures. It was below 60% at 20℃ treatments of July to September. On the other hand, a uniform budbreak was observed in the treatments after the middle of endodormancy for all temperatures, resulting in almost 100% of final percentage of budbreak. There was a significant negative correlation between NDIB and cumulative chilling hour (CCH) of exposure to below 7.2℃ in the treatments after November, and also between NDIB and cumulative temperature (CT, ℃･h), a summation of temperature and hours of exposure to above 0°C from November 1 to each treatment time and hours of exposure to 20, 25, or 30℃ from start of treatment
to budbreak in each plot. The results suggest that besides CCH, CT can also be used to estimate the completion of dormancy in 'Pione' grapevine bud.
en-copyright=
kn-copyright=

en-aut-name=PotjanapimonChaiwat
en-aut-sei=Potjanapimon
en-aut-mei=Chaiwat
kn-aut-name=ポジャナピモンチャイワット
kn-aut-sei=ポジャナピモン
kn-aut-mei=チャイワット
aut-affil-num=1
ORCID=

en-aut-name=FukudaFumio
en-aut-sei=Fukuda
en-aut-mei=Fumio
kn-aut-name=福田文夫
kn-aut-sei=福田
kn-aut-mei=文夫
aut-affil-num=2
ORCID=

en-aut-name=KubotaNaohiro
en-aut-sei=Kubota
en-aut-mei=Naohiro
kn-aut-name=久保田尚浩
kn-aut-sei=久保田
kn-aut-mei=尚浩
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=breaking of grapevine bud
kn-keyword=breaking of grapevine bud
en-keyword=cumulative chilling hour (CCH)
kn-keyword=cumulative chilling hour (CCH)
en-keyword=cumulative temperature (CT)
kn-keyword=cumulative temperature (CT)
en-keyword=different dormant stages
kn-keyword=different dormant stages
en-keyword=temperature
kn-keyword=temperature
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=27
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of FFC Ceramic Water on the Infection Process of a Fungal Pathogen
kn-title=病原菌の感染行動に及ぼす FFC セラミック水の効果について
en-subtitle=
kn-subtitle=
en-abstract=本研究は，FFCセラミックス(TM)（株 エフフシージャパン）の植物病原菌の発病抑制効果について調べたものである．FFCセラミック水は原液の1/2～1/6の濃度でエンドウ褐紋病菌の発病を顕著に抑制した．この原因を調べたところ，FFCセラミック水は，病原菌の発芽，発芽管伸長，侵入（貫入）を顕著に阻害することが判明した．FFCセラミック水中にはCa並びにS，O元素が多量に存在し，SEM観察の結果と合わせると，CaSO(4)が多量に含まれることが示唆された．そこで，CaSO(4)飽和液の1/2～1/4濃度で，病原菌に対する作用を調べた結果，発芽あるいは発芽管伸長はほとんど阻害されず，低率ながら侵入も観察された．これらの結果を総合して，FFCセラミック水やCaSO4の栽培場面での応用を考察した．
kn-abstract=In this report, an effect of FFC-ceramic (FFC-Japan Co. Ltd., Tsu) water on the process of infection by a pea fungal pathogen, Mycosphaerella pinodes was investigated. Energy dispersive X-ray analysis showed that both of the FFC-ceramic water and a common ceramic water contained mainly Ca and S elements, of which the relative atomic percentages were 53~56% and 44~45%, respectively. Lesion formation by pycnospores of M. pinodes on pea leaves was inhibited severely by the application with both ceramic waters at the 1/2~1/6 concentration of saturated solution. Cytological observation under microscope showed that germination, germ-tube elongation and penetration were severely inhibited by these ceramic waters. However, such inhibitory effect of FFC-ceramic water was superior to that of the common ceramic water. On ethanol-killed pea epidermal tissues, both FFC-ceramic water and the common ceramic water blocked the germination, germ-tube elongation and penetration by the pathogen, indicating
the direct effect of both ceramic waters on the fungus. In this case, the inhibiting effect of FFC ceramic water was more intensive than the common ceramic water. CaSO(4) at a 1/2~1/4 concentration of saturated solution blocked penetration by the fungus on the killed epidermis of onion bulb but scarcely affected germination and germ-tube elongation. Based on these results, we discussed the role of FFC-ceramic water in disease tolerance of plants and its availability for cultivation.
en-copyright=
kn-copyright=

en-aut-name=ShiraishiTomonori
en-aut-sei=Shiraishi
en-aut-mei=Tomonori
kn-aut-name=白石友紀
kn-aut-sei=白石
kn-aut-mei=友紀
aut-affil-num=1
ORCID=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=豊田和弘
kn-aut-sei=豊田
kn-aut-mei=和弘
aut-affil-num=2
ORCID=

en-aut-name=SuzukiTomoko
en-aut-sei=Suzuki
en-aut-mei=Tomoko
kn-aut-name=鈴木智子
kn-aut-sei=鈴木
kn-aut-mei=智子
aut-affil-num=3
ORCID=

en-aut-name=MeguroAkane
en-aut-sei=Meguro
en-aut-mei=Akane
kn-aut-name=目黒あかね
kn-aut-sei=目黒
kn-aut-mei=あかね
aut-affil-num=4
ORCID=

en-aut-name=HasegawaSachiko
en-aut-sei=Hasegawa
en-aut-mei=Sachiko
kn-aut-name=長谷川幸子
kn-aut-sei=長谷川
kn-aut-mei=幸子
aut-affil-num=5
ORCID=

en-aut-name=NishimuraTomio
en-aut-sei=Nishimura
en-aut-mei=Tomio
kn-aut-name=西村富生
kn-aut-sei=西村
kn-aut-mei=富生
aut-affil-num=6
ORCID=

en-aut-name=KunohHitoshi
en-aut-sei=Kunoh
en-aut-mei=Hitoshi
kn-aut-name=久能均
kn-aut-sei=久能
kn-aut-mei=均
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=株式会社赤塚植物園・生物機能開発研究所

affil-num=5
en-affil=
kn-affil=株式会社赤塚植物園・生物機能開発研究所

affil-num=6
en-affil=
kn-affil=株式会社赤塚植物園・生物機能開発研究所

affil-num=7
en-affil=
kn-affil=岡山大学
en-keyword=Calcium sulfate
kn-keyword=Calcium sulfate
en-keyword=FFC-ceramics
kn-keyword=FFC-ceramics
en-keyword=infection establishment
kn-keyword=infection establishment
en-keyword=Mycosphaerella pinodes
kn-keyword=Mycosphaerella pinodes
en-keyword=Pisum sativum
kn-keyword=Pisum sativum
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=21
end-page=26
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=FFC Ceramic Water(TM) Enhances Plant Apyrase Activity
kn-title=FFC セラミック水(TM)による植物アピラーゼの活性化作用
en-subtitle=
kn-subtitle=
en-abstract=本報は，FFC セラミックス(TM)（㈱エフエフシー・ジャパン）で調製した FFC セラミック水（FFC 水）の植物アピラーゼ（EC 3.6.1.5）の活性に及ぼす直接的な作用について調べたものである．FFC 水はアピラーゼがもつ ATP加水分解活性を促進し，その作用は反応液への添加量に依存した．先の無機元素分析結果から，FFC 水に含まれる主要な塩類はCa(2＋)であることが判明している．そこで，Ca(2＋)キレート剤EGTAを反応液へ加え，その影響について調べたところ，FFC 水による活性化作用は消失することが明らかとなった．また，FFC 水と類似の作用は，硫酸カルシウム，塩化カルシウムまたは硝酸カルシウムの添加で認められ，陰イオンの種類によって明確な違いはなかった．これらの結果から，FFC 水が植物アピラーゼに及ぼす活性化作用の一因は，セラミックスから遊離する Ca(2＋)に依存しているものと推察された．一方，アピラーゼ活性を指標として，使用済のセラミックスから調製した FFC 水の効果について検討したところ，未使用からの水と比べて，カルシウム濃度ならびに活性化作用の顕著な低下が認められた．このことは，継続的な使用によってセラミックスから遊離する塩類，特にカルシウムの溶出量が大きく変わることを意味し，アピラーゼを用いた本検定が，FFC 水の効果を定量的に確かめる方法の一つとして利用できると考えられた．以上，これらの結果を総合して，FFC水の植物酵素への直接的作用，ならびに植物への施用によって効果が現れる耐病性獲得作用との関連について考察した．
kn-abstract=The FFC ceramics(TM) from FFC Japan Co., Ltd. are now widely used in the fields of agriculture, fishery and food industry in Japan. Recently the FFC ceramic beads-based technology has been also applied to meet several environmental problems including pollution in sea, lakes and rivers. In this study the FFC ceramic water was tested for effect on plant enzyme, potato apyrase (EC 3.6.1.5; ATP-diphosphohydrolase), which hydrolyses nucleoside triphosphate (NTP) and -diphosphate (NDP) to produce corresponding nucleoside monophosphate (NMP) and inorganic phosphate (Pi). Addition of the FFC ceramic water to the enzyme reaction mixture markedly enhanced ATP-hydrolyzing activity, when used as ATP as substrate. However, the concomitant presence of Ca(2+) chelator, EGTA (O,O'-bis(2-aminoethyl)ethyleneglycol-N,N,N',N'-tetraacetic acid) with the FFC ceramic water, completely abolished the enzyme activation. In fact, exogenous calcium ion such as CaSO4 mimicked the FFC ceramic water. These results indicate that apyrase activation by the FFC ceramic water largely depends on calcium ions. On the other hand, when the FFC ceramic water prepared from "used" ceramics was tested for the apyrase activity, the enhanced effect on apyrase was decreased compared to the FFC ceramic water from "new" ones. This result, consistent with our present data covering concentration of calcium ions and conductivity, indicates that long and/or successive usage of the ceramic beads results in decrease of contents of released minerals, especially calcium ions. The apyrase-based enzyme assay presented here is probably applicable to estimate and quantify the effect of FFC ceramic water.
en-copyright=
kn-copyright=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=豊田和弘
kn-aut-sei=豊田
kn-aut-mei=和弘
aut-affil-num=1
ORCID=

en-aut-name=MatsuokaSachiko
en-aut-sei=Matsuoka
en-aut-mei=Sachiko
kn-aut-name=松岡祥子
kn-aut-sei=松岡
kn-aut-mei=祥子
aut-affil-num=2
ORCID=

en-aut-name=MeguroAkane
en-aut-sei=Meguro
en-aut-mei=Akane
kn-aut-name=目黒あかね
kn-aut-sei=目黒
kn-aut-mei=あかね
aut-affil-num=3
ORCID=

en-aut-name=HasegawaSachiko
en-aut-sei=Hasegawa
en-aut-mei=Sachiko
kn-aut-name=長谷川幸子
kn-aut-sei=長谷川
kn-aut-mei=幸子
aut-affil-num=4
ORCID=

en-aut-name=NishimuraTomio
en-aut-sei=Nishimura
en-aut-mei=Tomio
kn-aut-name=西村富生
kn-aut-sei=西村
kn-aut-mei=富生
aut-affil-num=5
ORCID=

en-aut-name=KunohHitoshi
en-aut-sei=Kunoh
en-aut-mei=Hitoshi
kn-aut-name=久能均
kn-aut-sei=久能
kn-aut-mei=均
aut-affil-num=6
ORCID=

en-aut-name=ShiraishiTomonori
en-aut-sei=Shiraishi
en-aut-mei=Tomonori
kn-aut-name=白石友紀
kn-aut-sei=白石
kn-aut-mei=友紀
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=赤塚植物園生物機能開発研究所

affil-num=4
en-affil=
kn-affil=赤塚植物園生物機能開発研究所

affil-num=5
en-affil=
kn-affil=赤塚植物園生物機能開発研究所

affil-num=6
en-affil=
kn-affil=岡山大学

affil-num=7
en-affil=
kn-affil=岡山大学
en-keyword=apyrase
kn-keyword=apyrase
en-keyword=ATP-diphosphohydrolase
kn-keyword=ATP-diphosphohydrolase
en-keyword=calcium ion
kn-keyword=calcium ion
en-keyword=FFC ceramic(TM)
kn-keyword=FFC ceramic(TM)
en-keyword=FFC ceramic water(TM)
kn-keyword=FFC ceramic water(TM)
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=13
end-page=20
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=土壌改質材 FFC エースによるオオムギの生育と収量の促進効果
kn-title=Enhancement of Growth and Yield of Barley by the Soil Conditioner FFC-ace
en-subtitle=
kn-subtitle=
en-abstract=本報は，㈱エフエフシー・ジャパンから販売されている土壌改質材FFCエースTMの作物の生長促進効果について，2006年11月から翌年６月，本学農学部内の実験圃場で実施された，オオムギの生育ならびに収量調査に関する試験結果をとりまとめたものである．実施圃場の砂土壌にFFCエースを所定量混和した区画を設け，オオムギの種子を播種した．なお，対照区は非導入土壌とした．定期的に行った生育調査の結果，FFCエースを導入した土壌では非導入の区画と比べて，生育初期における根の生育が良好となり，地上部における分けつ数の増加とともに穂の生長も旺盛となって，１穂当たりの収穫量（粒数）の著しい増加をもたらした．結果，FFCエース導入区における全収量は非導入区と比べて約1.7倍となった．また，それぞれから収穫したオオムギ粒に含まれる栄養価ならびに無機元素類の量には，FFCエースの導入，非導入によって大きな違いは認められず，導入の効果は収量に大きく反映された．事実，調査期間中に行った測定から，FFCエースを投入した土壌で生育するオオムギ葉は高いクロロフィル量を示しており，光合成が促進されているものと考えられた．実際，播種後４ヶ月目以降，光合成ならびに蒸散速度値を測定した結果，FFCエース導入区で生育したオオムギでは常に高い値を示した．また，FFCエースの導入によって強光条件下における水利用効率が促進された．本報告では，FFCエースの投与と空気中からの二酸化炭素の吸収量との関連について考察するとともに，併せて，FFCエースの土壌への導入によって作物の生育に必要な灌水量を大きく減らすことができる可能性についても言及したい．
kn-abstract=The effects of a unique soil conditioner, FFC-ace, on photosynthesis, transpiration, growth and yield of barley were examined in a field experiment. FFC-ace well-mixed with sandy soil greatly enhanced root and shoot growth, tillering and the number of grains per stock. The total yield in the treated plot increased by about 172%. The plants grown in the FFC-ace plot were greener and contained a higher level of chlorophyll, compared with the control. Photosynthesis and transpiration, which are tightly linked to productivity were also significantly enhanced at the broad range of photon flux observed in our study. The quality of grain harvested from the FFC-ace plot was similar to the control plot in terms of nutritional and inorganic components. The increased photosynthesis in the FFC-ace treated barley reflects a higher absorption of CO(2) from the atmosphere. It was also noted that the efficiency of water utilization for photosynthesis was significantly greater under the high light intensity in the treated plot. The relationship between application of FFC-ace and absorption of atmospheric CO(2) is discussed. Our investigation provides
data showing that application of FFC-ace to soil significantly reduces water requirements for plant growth and yield.
en-copyright=
kn-copyright=

en-aut-name=FujitaKeiko
en-aut-sei=Fujita
en-aut-mei=Keiko
kn-aut-name=藤田景子
kn-aut-sei=藤田
kn-aut-mei=景子
aut-affil-num=1
ORCID=

en-aut-name=SuzukiTomoko
en-aut-sei=Suzuki
en-aut-mei=Tomoko
kn-aut-name=鈴木智子
kn-aut-sei=鈴木
kn-aut-mei=智子
aut-affil-num=2
ORCID=

en-aut-name=HasegawaSachiko
en-aut-sei=Hasegawa
en-aut-mei=Sachiko
kn-aut-name=長谷川幸子
kn-aut-sei=長谷川
kn-aut-mei=幸子
aut-affil-num=3
ORCID=

en-aut-name=MeguroAkane
en-aut-sei=Meguro
en-aut-mei=Akane
kn-aut-name=目黒あかね
kn-aut-sei=目黒
kn-aut-mei=あかね
aut-affil-num=4
ORCID=

en-aut-name=SugiuraHiroyuki
en-aut-sei=Sugiura
en-aut-mei=Hiroyuki
kn-aut-name=杉浦裕幸
kn-aut-sei=杉浦
kn-aut-mei=裕幸
aut-affil-num=5
ORCID=

en-aut-name=ToyodaKazuhiro
en-aut-sei=Toyoda
en-aut-mei=Kazuhiro
kn-aut-name=豊田和弘
kn-aut-sei=豊田
kn-aut-mei=和弘
aut-affil-num=6
ORCID=

en-aut-name=ShiraishiTomonori
en-aut-sei=Shiraishi
en-aut-mei=Tomonori
kn-aut-name=白石友紀
kn-aut-sei=白石
kn-aut-mei=友紀
aut-affil-num=7
ORCID=

en-aut-name=SakaguchiEi
en-aut-sei=Sakaguchi
en-aut-mei=Ei
kn-aut-name=坂口英
kn-aut-sei=坂口
kn-aut-mei=英
aut-affil-num=8
ORCID=

en-aut-name=NishimuraTomio
en-aut-sei=Nishimura
en-aut-mei=Tomio
kn-aut-name=西村富生
kn-aut-sei=西村
kn-aut-mei=富生
aut-affil-num=9
ORCID=

en-aut-name=KunohHitoshi
en-aut-sei=Kunoh
en-aut-mei=Hitoshi
kn-aut-name=久能均
kn-aut-sei=久能
kn-aut-mei=均
aut-affil-num=10
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=㈱赤塚植物園・生物機能開発研究所

affil-num=6
en-affil=
kn-affil=岡山大学

affil-num=7
en-affil=
kn-affil=岡山大学

affil-num=8
en-affil=
kn-affil=岡山大学

affil-num=9
en-affil=
kn-affil=㈱赤塚植物園・生物機能開発研究所

affil-num=10
en-affil=
kn-affil=岡山大学
en-keyword=barley (Hordeum vulgare L.)
kn-keyword=barley (Hordeum vulgare L.)
en-keyword=enhanced growth and yield
kn-keyword=enhanced growth and yield
en-keyword=FFC-ace (soil conditioner)
kn-keyword=FFC-ace (soil conditioner)
en-keyword=enhanced photosynthesis and transpiration
kn-keyword=enhanced photosynthesis and transpiration
en-keyword=chemical analysis of grains
kn-keyword=chemical analysis of grains
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=7
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Purification and Characterization of Cystathionine γ-Synthase from Thermoacidophilic Archaea Sulfolobus tokodaii
kn-title=好熱好酸性アーキア Sulfolobus tokodaii 由来シスタチオニン γ-シンターゼの精製及び性質検討
en-subtitle=
kn-subtitle=
en-abstract=好熱好酸性アーキア Sulfolobus tokodaii 由来シスタチオニンγﾝシンターゼ（stCGS）遺伝子を pET-11a に組み込み pET-stCGS を構築した．このベクターでE. coli Rosettaﾝgami（DE3）を形質転換し，本遺伝子を発現させ，精製及び性質検討を行った．大腸菌で発現したシスタチオニンγﾝシンターゼの活性が無細胞抽出液で確認できた．S. tokodaii シスタチオニンγﾝシンターゼを70℃熱処理 DEAEﾝトヨパールイオン交換カラム等により単一精製した．精製酵素の最適温度は100℃以上であり，熱安定性は60分間処理で70℃までほぼ100ｵの残存活性を示した．また，最適pHについてはリン酸緩衝液やブリトンﾝロビンソン広域緩衝液の場合はpH7.0の時が最も活性が高く，トリス塩酸緩衝液の場合はpH9.0が最適であった．pH安定性についてはpH5.0～9.0において安定であった．O-ホスホ-l-ホモセリンに対するKm，Vmaxは，それぞれ0.82mM，2.42U/㎎であった．アポ酵素のホロ化実験により，本酵素活性がPLP に依存していることが明らかとなった．更に本酵素の脱離反応での基質特異性の検討を行った．変異酵素を用いた実験により，stCGSの基質特異性には，活性中心に存在するPhe97を含む領域が深く関わっていることが示唆された．
kn-abstract=The gene encoding a cystathionine γ-synthase from Sulfolobus tokodaii was cloned and expressed in Escherihia coli Rosetta-gami (DE3). Cystathionine γ-synthase ［EC 2. 5. 1. 48］ from Sulfolobus tokodaii (stCGS) was purified by heat treatment, DEAE- Toyopearl 650M and Sephacryl S-300 column chromatographies from E. coli transformants. stCGS shows optimum activity at pH 7.0, and is stable between pH5.0 and pH9.0. The optimum temperature of stCGS is above 100℃, and the enzyme showed the remaining activity of almost 100% up to 70℃. The K(m) and V(max) with O-phospho-L- homoserine as a substrate are 0.82 mM and 2.42 U/mg. To analyze the role of Phe 97 in the active site of stCGS, we constructed F97Y, R99C, and F97Y-R99C mutant enzymes. Although native stCGS has no activity toward l-methionine, F97Y mutant enzyme gained the elimination activity toward L-methionine.
en-copyright=
kn-copyright=

en-aut-name=ShinozakiMai
en-aut-sei=Shinozaki
en-aut-mei=Mai
kn-aut-name=篠崎舞
kn-aut-sei=篠崎
kn-aut-mei=舞
aut-affil-num=1
ORCID=

en-aut-name=YanagitaniMasahiko
en-aut-sei=Yanagitani
en-aut-mei=Masahiko
kn-aut-name=柳谷昌彦
kn-aut-sei=柳谷
kn-aut-mei=昌彦
aut-affil-num=2
ORCID=

en-aut-name=KanedaShouichirou
en-aut-sei=Kaneda
en-aut-mei=Shouichirou
kn-aut-name=兼田翔一郎
kn-aut-sei=兼田
kn-aut-mei=翔一郎
aut-affil-num=3
ORCID=

en-aut-name=KudouDaizou
en-aut-sei=Kudou
en-aut-mei=Daizou
kn-aut-name=工藤大蔵
kn-aut-sei=工藤
kn-aut-mei=大蔵
aut-affil-num=4
ORCID=

en-aut-name=EndouYuuichi
en-aut-sei=Endou
en-aut-mei=Yuuichi
kn-aut-name=遠藤祐一
kn-aut-sei=遠藤
kn-aut-mei=祐一
aut-affil-num=5
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=6
ORCID=

en-aut-name=KuramitsuSeiki
en-aut-sei=Kuramitsu
en-aut-mei=Seiki
kn-aut-name=倉光成紀
kn-aut-sei=倉光
kn-aut-mei=成紀
aut-affil-num=7
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=8
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岡山大学

affil-num=6
en-affil=
kn-affil=岡山大学

affil-num=7
en-affil=
kn-affil=大阪大学大学院理学研究科

affil-num=8
en-affil=
kn-affil=岡山大学
en-keyword=cystathionine γ-synthase
kn-keyword=cystathionine γ-synthase
en-keyword=pyridoxal 5’-phosphate
kn-keyword=pyridoxal 5’-phosphate
en-keyword=thermoacidophilic archaea
kn-keyword=thermoacidophilic archaea
en-keyword=Sulfolobus tokodaii
kn-keyword=Sulfolobus tokodaii
END

start-ver=1.4
cd-journal=joma
no-vol=99
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=5
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Identification of Ornithine-lactam Converted from Arginine in Streptomyces incarnatus NRRL8089
kn-title=シネフンギン生産菌Streptomyces incarnatus NRRL 8089由来アルギニン変換化合物「オルニチンラクタム」の同定
en-subtitle=
kn-subtitle=
en-abstract=シネフンギンは抗真菌，抗マラリア活性を有する核酸系抗生物質であり，放線菌 S. incarnatus により生合成される．シネフンギンはアデノシンとオルニチンがＣﾝＣ結合した構造であり，無細胞抽出液での取り込み実験からＬﾝアルギニンと ATP から生合成されると推測される．Ｌﾝアルギニン，Ｌﾝオルニチンを S. incarnatus の休止菌体反応系への投与を行いシネフンギン中間体の探索を行った．その結果50ﾋアルギニンは24時間以内に低極性化合物へと変換された．一方50ﾋオルニチンは変換されず反応液中に残存した．HPLC で化合物を精製し，1HﾝNMR，FABﾝMS での分析の結果オルニチン環状モノペプチド，「オルニチンラクタム」（分子量114）であることを明らかにした．この結果は S. incarnatus がアルギニンからオルニチンラクタムへの変換酵素を有する事を示唆する．このような酵素の報告例はこれまでになく，ニ次代謝酵素であることが示唆され，シネフンギン生合成との関連性に興味が持たれる．
kn-abstract=Sinefungin is a nucleoside antibiotic, in which a molecule of L-ornithine is linked to the 5' end of adenosine through a C-C bond. The antibiotic was isolated from the culture broth of Streptomyces incarnatus. For the purpose of detecting intermediate of sinefungin biosynthesis, resting cell suspensions were incubated with supplemental L-arginine, and L-ornithine. 50mM Arginine was converted to a compound X that has low polarity. 50mM ornithine was not converted and remained in reaction solution. Compound X was purified using HPLC, and analyzed using (1)H-NMR and FAB-MS. These analyses showed that a compound X is "ornithine-lactam" (Mw＝114), which has a structure of circularized ornithine. These results indicated that S. incarnatus has an enzyme that converts arginine to ornithine-lactam. Such an enzyme has never been reported, and suggested that it may be relevant to sinefungin biosynthesis.
en-copyright=
kn-copyright=

en-aut-name=FukudaKoji
en-aut-sei=Fukuda
en-aut-mei=Koji
kn-aut-name=福田康二
kn-aut-sei=福田
kn-aut-mei=康二
aut-affil-num=1
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=2
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学
en-keyword=sinefungin
kn-keyword=sinefungin
en-keyword=arginine
kn-keyword=arginine
en-keyword=ornithine
kn-keyword=ornithine
en-keyword=Streptomyces
kn-keyword=Streptomyces
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=59
end-page=76
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=The List of Papers Published by Members of the Faculty
from January to December 2008.
kn-title=公表学術論文等リスト・2008
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=岡山大学農学部
kn-aut-sei=岡山大学農学部
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=47
end-page=57
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Breeding, Feed, and Nutrition of Japanese Quail (Coturnix coturnix japonica)
kn-title=ウズラの育種ならびに飼料，栄養
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This report summarizes research achievements on the breeding, feed, and nutrition of Japanese quail performed at the Laboratory of Animal Genetics and Breeding, Department of Agriculture, Okayama University, between 1973 and 2008.
　The study included analysis of inbreeding depression and heterosis, which are important subjects in breeding. Secondly, the genetic parameters useful for selection were identified and the sexing of newborn chicks utilizing sex-linked inheritance was investigated. As problems related to feed and nutrition, the reduced excretion of nitrogen (N) and phosphorus (P), the utilization of a cochineal residue, and the effects of red salmon astaxanthin, palm oil, and astaxanthin supplementation on eggs functions were investigated.
　In quail, inbreeding depression was noted in overall productive characters, and the depression was marked in reproductive characters, such as hatchability. The genetic load was calculated using the equation: －logeS＝A＋BF. On crossbreeding between highly inbred lines of Japanese quail, marked heterosis was noted in the hatchability, viability,
and egg production rate.
　Analysis of early embryonic development showed that the number of mitotic cells, mitotic index, and 3H-thymidine and 3H-uridine uptakes were lower in the inbred embryos compared with those of hybrid embryos, indicating important
metabolic differences.
　Basic information for the selective breeding of quail, heritability of characters, such as the body weight, muscle mass, skeleton, and egg characteristics were accumulated; accumulated information suggested the possibility of selective
breeding with regard to these characters.
　Sexing of newborn quail chicks utilizing sex-linked inheritance was performed. Mating males of the brown line and females of the normal line, all male chicks had normal plumage colors, whereas all females had brown plumage colors, which allowed simple sexing.
The supplementation of low-CP feed with essential amino acids and low-CP, low-P feed with phytase improved the growth of chicks, increased the egg production rate, and reduced N and P excretions. Phytase supplementation enhanced tibial growth in the early growth period, and reduced perosis and the mortality of chicks. When female chicks were fed a cochineal residue at 6 weeks of age, the egg production rate was similar to that of chicks fed fish meal, showing the possibility of substitution with fish meal.
　The yolk color was improved by supplementation with red salmon astaxanthin, palm oil, and astaxanthin. The yolk color improved with increases in the supplemented amounts of palm oil and astaxanthin. Astaxanthin added to feed was transferred to the egg yolk, and the antioxidative capacity improved as the amount added increased.
　To further clarify the characteristics of quail as experimental animals, and to improve their productivity as livestock, a continuation in both basic and applied research is indispensable.
en-copyright=
kn-copyright=

en-aut-name=SatoKatsunori
en-aut-sei=Sato
en-aut-mei=Katsunori
kn-aut-name=佐藤勝紀
kn-aut-sei=佐藤
kn-aut-mei=勝紀
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=応用動物科学コース
en-keyword=Japanese quail
kn-keyword=Japanese quail
en-keyword=inbreeding depression
kn-keyword=inbreeding depression
en-keyword=heterosis
kn-keyword=heterosis
en-keyword=genetic parameters
kn-keyword=genetic parameters
en-keyword=N and P excretions
kn-keyword=N and P excretions
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=39
end-page=45
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Measurement and Analysis of Fog Flow in Plastic Greenhouse
kn-title=温室における煙霧流の測定と解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This paper deals with measurement and analysis of fog flow in a greenhouse.
　In this study we applied a gas-liquid two phase flow spray and a pesticide spray using new technology such as a non-heating fog carrier, in order to prevent desease and insects in agricultural operations.
　Spray particle density was analyzed by a new method using an air sampler, and the behavior of fog particles in the space was studied.
　The following results were obtained,
1. The spray particles were within a size of 15 ~ 30μm and the behavior of these particles were clarified in this experiment.
2. The floating time after spray of particles, and the diffusing performance of spray particles in the greenhouse were clearly observed, tracked and recorded in this experiment.
3. Effective methods of pesticide application in greenhouse were discovered in this study.
en-copyright=
kn-copyright=

en-aut-name=TangBomin
en-aut-sei=Tang
en-aut-mei=Bomin
kn-aut-name=湯伯敏
kn-aut-sei=湯
kn-aut-mei=伯敏
aut-affil-num=1
ORCID=

en-aut-name=MohriKentaro
en-aut-sei=Mohri
en-aut-mei=Kentaro
kn-aut-name=毛利建太郎
kn-aut-sei=毛利
kn-aut-mei=建太郎
aut-affil-num=2
ORCID=

en-aut-name=MontaMitsuji
en-aut-sei=Monta
en-aut-mei=Mitsuji
kn-aut-name=門田充司
kn-aut-sei=門田
kn-aut-mei=充司
aut-affil-num=3
ORCID=

en-aut-name=NambaKazuhiko
en-aut-sei=Namba
en-aut-mei=Kazuhiko
kn-aut-name=難波和彦
kn-aut-sei=難波
kn-aut-mei=和彦
aut-affil-num=4
ORCID=

en-aut-name=IwasakiMasami
en-aut-sei=Iwasaki
en-aut-mei=Masami
kn-aut-name=岩崎正美
kn-aut-sei=岩崎
kn-aut-mei=正美
aut-affil-num=5
ORCID=

en-aut-name=QiuBai-jing
en-aut-sei=Qiu
en-aut-mei=Bai-jing
kn-aut-name=邱白
kn-aut-sei=邱
kn-aut-mei=白
aut-affil-num=6
ORCID=

en-aut-name=LiangJian
en-aut-sei=Liang
en-aut-mei=Jian
kn-aut-name=梁建
kn-aut-sei=梁
kn-aut-mei=建
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=中国農業部南京農業機械化研究所

affil-num=2
en-affil=
kn-affil=岡山大学名誉教授

affil-num=3
en-affil=
kn-affil=環境生態学コース

affil-num=4
en-affil=
kn-affil=環境生態学コース

affil-num=5
en-affil=
kn-affil=鳥取大学

affil-num=6
en-affil=
kn-affil=中国江蘇大学

affil-num=7
en-affil=
kn-affil=中国農業部南京農業機械化研究所
en-keyword=egg
kn-keyword=egg
en-keyword=food allergy
kn-keyword=food allergy
en-keyword=IgE
kn-keyword=IgE
en-keyword=mast cells
kn-keyword=mast cells
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=31
end-page=37
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Animal Experiment on Functional Features of Eggs Stated to be
Hypoallergenic for People with Food Allergies
kn-title=低アレルギー発症性が言及されている鶏卵の機能特性に関する動物実験
en-subtitle=
kn-subtitle=
en-abstract=食物アレルギーを持つ消費者の経験に基づいて低アレルギー誘発性であることが言及されている鶏卵について，低アレルギー誘発性の有無と科学的な根拠を明らかにするために動物を用いた実験をおこなった．実験にはアレルギーを発症することが知られているラットの系統であるBrown Norway rat（BNラット）を用い，この鶏卵を混ぜ込んだ飼料で飼育した．対照として，市販の鶏卵（普通卵）で同様に飼育した．試験卵で飼育したBNラットでは，空腸と回腸組織のIgE陽性細胞の密度は普通卵で飼育した場合に比較して大きく低下した．組織中のIgE陽性細胞の大部分は肥満細胞であることが知られていることから試験卵群における小腸組織の肥満細胞密度は低下した．さらに，血中の好酸球数は同様に試験卵による飼育で低下した．一方，血清のIgE濃度には摂取させた卵による違いは認められなかった．以上の結果は，試験卵を摂取した場合，通常の卵の摂取に比較して即時型アレルギー症状の発生に直接的に関与する肥満細胞と好酸球の誘導や増殖が促進されにくいと考えられ，このことが，試験卵がアレルギーを引き起こしにくい一因であると推察される．
kn-abstract=Functional features concerned with low proallergic natures were examined using an allergy-inducible rat strain (Brown Norway rat; BN rat) on hen's eggs which have been empirically mentioned as hypoallergenic
for patients suffering from food allergies (experimental eggs). BN rats were fed on feed containing
whole experimental eggs (feed E) and whole normal eggs (control feed, feed C). The densities of immunoglobulin E (IgE)-positive cells, have reported to be IgE-bearing mast cells, in the jejunum and ileum of BN rats fed on experimental-egg-containing feed were lower than those of BN rats fed on normal-egg-containing feed. The number of blood eosinophils was also lower in BN rats fed on feed E. Serum IgE levels were no different between BN rats fed on feed E and feed C. These results indicate that the low proallergic nature of hen's eggs studied in the present study is due to the dereased ability of experimental eggs to facilitate the proliferation and induction of mast cells in the intestinal tissue.
en-copyright=
kn-copyright=

en-aut-name=NagatoKumiko
en-aut-sei=Nagato
en-aut-mei=Kumiko
kn-aut-name=長門久美子
kn-aut-sei=長門
kn-aut-mei=久美子
aut-affil-num=1
ORCID=

en-aut-name=AbeAsaki
en-aut-sei=Abe
en-aut-mei=Asaki
kn-aut-name=阿部浅樹
kn-aut-sei=阿部
kn-aut-mei=浅樹
aut-affil-num=2
ORCID=

en-aut-name=NarabaraKiyoaki
en-aut-sei=Narabara
en-aut-mei=Kiyoaki
kn-aut-name=楢原清顕
kn-aut-sei=楢原
kn-aut-mei=清顕
aut-affil-num=3
ORCID=

en-aut-name=KondoYasuhiro
en-aut-sei=Kondo
en-aut-mei=Yasuhiro
kn-aut-name=近藤康博
kn-aut-sei=近藤
kn-aut-mei=康博
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=応用動物科学コース

affil-num=2
en-affil=
kn-affil=応用動物科学コース

affil-num=3
en-affil=
kn-affil=応用動物科学コース

affil-num=4
en-affil=
kn-affil=応用動物科学コース
en-keyword=egg
kn-keyword=egg
en-keyword=food allergy
kn-keyword=food allergy
en-keyword=IgE
kn-keyword=IgE
en-keyword=mast cells
kn-keyword=mast cells
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=23
end-page=29
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Large-fruited Tomato Production as Affected by Root-zone Extension and Wick Addition During Cultivation in a Capillary Wick System
kn-title=大玉トマトの防根給水ひも栽培における生育途中の根域拡張と「ひも」適用が果実生産に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=「防根給水ひも」を利用した底面給水法が大玉トマト生産に適用可能かどうかを検討した．2006年秋ﾝ冬季の実験では，培地量がトマトの生育と収量に及ぼす影響について調査した．大玉トマトを終始1stBox（＝2.8ℓ･plant－1）で栽培する処理区と７段花房開花時に2ndBox（＝2.8ℓ）に培地を追加し，最終的に１stBox + 2ndBox（＝5.6ℓ･plant－1）で栽培する処理区を比較した結果，培地量を２倍にし，根域を拡張した処理区の収量が高くなった．2007年春ﾝ夏季の実験では根域拡張および「ひも」適用本数の増加によりトマトの生育収量を改善出来るかを検討するため，終始 1stBox，「ひも」1本で栽培する区，4段花房開花時に培地を追加し最終的に1stBox + 2ndBoxで栽培するが，そこには「ひも」は配さない区，培地を加えると同時に「ひも」を配する区の3区でトマトを栽培した．その結果，1stBox + 2ndBox，「ひも」2本で栽培したトマトが最も収量が高かった．培地追加時に「ひも」を配することで水輸送量が増大した事が収量増加に繋がったものと考えられた．以上の結果より，生育途中で培地量を増やすと同時に，もう1本の「ひも」を配置して大塚A処方1/2濃度で終始管理すれば，生育中期にK欠乏と思しき葉縁の黄変が見られるものの，尻腐れ果実のほとんど発生しない安定したトマト生産が可能であるといえる．
kn-abstract=This study was conducted to investigate the possibility to use capillary wick system in large-fruited tomato production. The first experiment in the autumn-winter season of 2006 was carried out to investigate
the effects of the amount of substrate on growth and yield of tomato. Treatments involved growing large-fruited tomato in one box (2.8ℓ/plant) continuously, or adding substrate at flowering of the 7th truss in a 2nd box (5.6ℓ/plant). Fruit yield was higher when the root-zone was extended with double the substrate volume. The second experiment in spring-summer season of 2007 was aimed at improving growth and yield of tomato by extension of root-zone and addition of wick. Tomato plants were either grown in one box with one wick continuously, or with addition of substrate in a 2nd box at flowering of the 4th truss. Additionally, one more wick was inserted into 50% of the 2nd boxes. Half of the plants were grown in two boxes with one wick, and the other half with two wicks. Higher yield was obtained from the plants grown in two boxes with two wicks, suggesting that fruit yield was increased by increasing water transport through wick addition coupled with root-zone extension. Plant growth and fruit yield of large-fruited tomato was stable without blossom-end rot when root-zone was extended and half strength of Ohtsuka-A nutrient solution supplied through the capillary wick system. There was, however, a slight sign of physiological disorder at the leaf margins similar to potassium deficiency.
en-copyright=
kn-copyright=

en-aut-name=MorishigeAyuki
en-aut-sei=Morishige
en-aut-mei=Ayuki
kn-aut-name=森重歩己
kn-aut-sei=森重
kn-aut-mei=歩己
aut-affil-num=1
ORCID=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=2
ORCID=

en-aut-name=MurakamiKenji
en-aut-sei=Murakami
en-aut-mei=Kenji
kn-aut-name=村上賢治
kn-aut-sei=村上
kn-aut-mei=賢治
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=応用植物科学コース

affil-num=2
en-affil=
kn-affil=応用植物科学コース

affil-num=3
en-affil=
kn-affil=応用植物科学コース
en-keyword=root-proof capillary wick
kn-keyword=root-proof capillary wick
en-keyword=large-fruited tomato
kn-keyword=large-fruited tomato
en-keyword=substrate volume
kn-keyword=substrate volume
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=17
end-page=22
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of Combination of Overhead Irrigation and Subirrigation on the Growth of Bedding Plants Grown in Polyester Fiber Medium Hardened by Heat Fusion, without use of Polyethylene Pots
kn-title=頭上灌水と底面灌水の組み合わせが熱融着性ポリエステル繊維固化
ポットレス培地で育てた花壇苗の生育に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=熱融着性ポリエステル繊維固化培地を利用したポットレス花壇苗生産における灌水方法として，水受けトレイを用いた頭上灌水（頭上底面灌水）の実用性を検討した．頭上灌水と比較して頭上底面灌水では灌水効率（培地に吸収された灌水量／灌水量）が大幅に改善できた．ガーデンシクラメンの生育は頭上底面灌水区で最もよく，次いでひも灌水，底面灌水の順であり，頭上灌水区で最も生育が抑制された．ニチニチソウでは頭上灌水より頭上底面灌水区で生育が改善されたが，灌水量には差はほとんどみられなかった．ペチュニアでは灌水量が多いほど，専用トレイに溜める水量が多いほど生育が旺盛になった．しかし，パンジーでは頭上底面灌水によって生育が抑制された．ポットレス花壇苗生産では頭上底面灌水が最も適切であったが，蒸発散の少ない冬期や過湿に弱い種においては灌水量をできるだけ少なくすべきと考えられた．
kn-abstract=The effects of irrigation methods on several bedding plants without polyethylene pots using compacted
polyester fiber medium hardened by heat fusion were investigated. The characteristics of overhead
irrigation with a tray collecting runoff water (combination of overhead irrigation and subirrigation)
were investigated at the same time. Irrigation usage (the amount of water supplied per pot / amount of irrigation water) with a combination of overhead irrigation and subirrigation was improved compared to overhead irrigation alone. The growth of garden type Cyclamen grown under a combination of both overhead irrigation and subirrigation was significantly greater than that grown in either wick irrigation, ebb & flow irrigation or overhead irrigation. Notably, smaller growth was achiered in garden type cyclamen grown with overhead irrigation. The growth of Vinca grown in a combination of overhead irrigation and subirrigation was significantly greater than that grown with overhead irrigation alone. The irrigation amount had no effect on the growth of Vinca regardless of irrigation method. The growth of Petunia was greater with increased amounts of irrigation water. However, the growth of Pansy grown under overhead irrigation was significantly greater than that grown in a combination of overhead
irrigation and subirrigation. These results suggest that a combination of overhead irrigation and subirrigation method in bedding plant production without polyethylene pots is generally suitable since the plants grow without drought stress. However, it may be suggested that the amount of irrigation water should be as low as possible in the winter season or with sensitive species in order to prevent waterlogging injury.
en-copyright=
kn-copyright=

en-aut-name=OohashiYuji
en-aut-sei=Oohashi
en-aut-mei=Yuji
kn-aut-name=大橋佑司
kn-aut-sei=大橋
kn-aut-mei=佑司
aut-affil-num=1
ORCID=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=2
ORCID=

en-aut-name=ShimizuNozomi
en-aut-sei=Shimizu
en-aut-mei=Nozomi
kn-aut-name=清水希
kn-aut-sei=清水
kn-aut-mei=希
aut-affil-num=3
ORCID=

en-aut-name=KomaYasushi
en-aut-sei=Koma
en-aut-mei=Yasushi
kn-aut-name=小間康史
kn-aut-sei=小間
kn-aut-mei=康史
aut-affil-num=4
ORCID=

en-aut-name=MorishitaTeruhisa
en-aut-sei=Morishita
en-aut-mei=Teruhisa
kn-aut-name=森下照久
kn-aut-sei=森下
kn-aut-mei=照久
aut-affil-num=5
ORCID=

en-aut-name=FujiiKazunori
en-aut-sei=Fujii
en-aut-mei=Kazunori
kn-aut-name=藤井一徳
kn-aut-sei=藤井
kn-aut-mei=一徳
aut-affil-num=6
ORCID=

en-aut-name=ShimaKohji
en-aut-sei=Shima
en-aut-mei=Kohji
kn-aut-name=島浩二
kn-aut-sei=島
kn-aut-mei=浩二
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=みのる産業㈱植物工学研究所

affil-num=2
en-affil=
kn-affil=応用植物科学コース

affil-num=3
en-affil=
kn-affil=応用植物科学コース

affil-num=4
en-affil=
kn-affil=みのる産業(株)植物工学研究所

affil-num=5
en-affil=
kn-affil=みのる産業(株)植物工学研究所

affil-num=6
en-affil=
kn-affil=みのる産業(株)植物工学研究所

affil-num=7
en-affil=
kn-affil=和歌山県農林水産総合技術センター農業試験場
en-keyword=bedding plant
kn-keyword=bedding plant
en-keyword=combination of overhead irrigation and subirrigation
kn-keyword=combination of overhead irrigation and subirrigation
en-keyword=drought stress
kn-keyword=drought stress
en-keyword=irrigation usage
kn-keyword=irrigation usage
en-keyword=waterlogging injury
kn-keyword=waterlogging injury
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=16
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Estimation of Completion of Dormancy in Grapevine Bud Based on Cumulative Temperature
kn-title=積算温度によるブドウの芽の休眠完了予測
en-subtitle=
kn-subtitle=
en-abstract=ブドウの芽の休眠完了と温度との関係を調査するため，温度条件の異なる７園（中信農試，山梨果試，岡山農試，岡山農試北部支場，岡山大学，福岡農試および宮崎）で栽培されている‘巨峰’と‘ピオーネ’から低温遭遇量の異なる３時期（12月，１月，２月）に結果母枝を採取した．直ちに岡山大学に送り，１芽を持つ挿し穂に調整した後，25または30℃のインキュベーター（14時間日長）に入れ，２日間隔で60日間発芽を調査した．処理開始時の7.2℃以下の遭遇量は中信農試で最も多く，次いで岡山農試北部支場，山梨果試，岡山農試，岡山大学，福岡農試，宮崎の順で，中信農試と宮崎では処理時期により2.5～4.8倍の差があった．発芽所要日数は，両品種とも処理時期が遅いほど，また温度が高いほど少なく，60％発芽所要日数もほぼ同様の傾向であった．‘巨峰’の発芽所用日数は中信農試，岡山大学および宮崎で少ない一方，岡山農試，山梨果試および福岡農試で多く，また‘ピオーネ’では福岡農試と岡山大学で少なく，山梨果試と岡山農試で多かった．７園のうち４園で低温遭遇量と発芽所要日数との間に負の相関がみられたが，相関係数は低かった．一方，11月以降処理開始までの０℃以上の温度の積算値と処理開始から発芽までの25または30℃での積算値を合計した積算温度と発芽所要日数との間には１園を除き極めて高い正の相関が認められた．これらの結果を基に，積算温度によるブドウの休眠完了予測の可能性を考察した．
kn-abstract=　This study was conducted to investigate the relationship between completion of dormancy of grapevine
bud and temperature. Canes of ‘Kyoho’ and ‘Pione’ grapevines (Vitis labrusca×V. vinifera) grown in 7 vineyards with different temperature conditions, in Nagano, Northern Okayama, Yamanashi, Okayama, Okayama University, Fukuoka and Miyazaki, were collected at three different chilling exposures,
December, January and February. These were then sent to Okayama University all at the same time. Cuttings with one bud were put into growth chambers kept at 25 or 30°C with 14 hours daylength, and budbreak in each cutting was surveyed at two day intervals for 60 days. Cumulative chilling hours (CCH) of exposure to below 7.2°C in each treatment time was largest in Nagano, followed in order by Northern Okayama, Yamanashi, Okayama, Okayama University, Fukuoka and Miyazaki. The CCH in Nagano was 2.5 to 4.8 times larger than in Miyazaki depending on the treatment time. The later the treatment time and the higher the temperature, the fewer were the number of days to first budbreak (NDFB) after treatment, irrespective of cultivar. A similar trend was observed in the number of days to 60% budbreak. In ‘Kyoho’ the NDFB was short in Nagano, Okayama University and Miyazaki, and longer in Okayama, Yamanashi and Fukuoka. In ‘Pione’ the NDFB was short in Fukuoka and Okayama University, and longer in Yamanashi and Okayama. The result was a weak negative correlation observed between CCH and NDFB in 4 of 7 vineyards. However, there was a strong positive correlation between NDFB and cumulative temperature (CT), a summation of temperature and hours of exposure to above 0°C from November 1 to treatment time and hours of exposure to 25 or 30°C from start of treatment to budbreak in each plot, in 6 vineyards excluding Miyazaki. The importance of estimating the completion of dormancy in grapevine bud based on CT is discussed.
en-copyright=
kn-copyright=

en-aut-name=KubotaNaohiro
en-aut-sei=Kubota
en-aut-mei=Naohiro
kn-aut-name=久保田尚浩
kn-aut-sei=久保田
kn-aut-mei=尚浩
aut-affil-num=1
ORCID=

en-aut-name=PotjanapimonChaiwat
en-aut-sei=Potjanapimon
en-aut-mei=Chaiwat
kn-aut-name=ポジャナピモンチャイワット
kn-aut-sei=ポジャナピモン
kn-aut-mei=チャイワット
aut-affil-num=2
ORCID=

en-aut-name=FukudaFumio
en-aut-sei=Fukuda
en-aut-mei=Fumio
kn-aut-name=福田文夫
kn-aut-sei=福田
kn-aut-mei=文夫
aut-affil-num=3
ORCID=

en-aut-name=FujiiaYuichiro
en-aut-sei=Fujiia
en-aut-mei=Yuichiro
kn-aut-name=藤井雄一郎
kn-aut-sei=藤井
kn-aut-mei=雄一郎
aut-affil-num=4
ORCID=

en-aut-name=OnoToshiro
en-aut-sei=Ono
en-aut-mei=Toshiro
kn-aut-name=小野俊朗
kn-aut-sei=小野
kn-aut-mei=俊朗
aut-affil-num=5
ORCID=

en-aut-name=KurafujiYuki
en-aut-sei=Kurafuji
en-aut-mei=Yuki
kn-aut-name=倉藤祐輝
kn-aut-sei=倉藤
kn-aut-mei=祐輝
aut-affil-num=6
ORCID=

en-aut-name=OgoroAtsuo
en-aut-sei=Ogoro
en-aut-mei=Atsuo
kn-aut-name=尾頃敦郎
kn-aut-sei=尾頃
kn-aut-mei=敦郎
aut-affil-num=7
ORCID=

en-aut-name=KunugiYukihiro
en-aut-sei=Kunugi
en-aut-mei=Yukihiro
kn-aut-name=功刀幸博
kn-aut-sei=功刀
kn-aut-mei=幸博
aut-affil-num=8
ORCID=

en-aut-name=KobayashiKazushi
en-aut-sei=Kobayashi
en-aut-mei=Kazushi
kn-aut-name=小林和司
kn-aut-sei=小林
kn-aut-mei=和司
aut-affil-num=9
ORCID=

en-aut-name=ShigeharaIzumi
en-aut-sei=Shigehara
en-aut-mei=Izumi
kn-aut-name=茂原泉
kn-aut-sei=茂原
kn-aut-mei=泉
aut-affil-num=10
ORCID=

en-aut-name=YamashitaHiroyuki
en-aut-sei=Yamashita
en-aut-mei=Hiroyuki
kn-aut-name=山下裕之
kn-aut-sei=山下
kn-aut-mei=裕之
aut-affil-num=11
ORCID=

en-aut-name=FujishimaHiroyuki
en-aut-sei=Fujishima
en-aut-mei=Hiroyuki
kn-aut-name=藤島宏之
kn-aut-sei=藤島
kn-aut-mei=宏之
aut-affil-num=12
ORCID=

affil-num=1
en-affil=
kn-affil=応用植物科学コース

affil-num=2
en-affil=
kn-affil=応用植物科学コース

affil-num=3
en-affil=
kn-affil=応用植物科学コース

affil-num=4
en-affil=
kn-affil=岡山県農業総合センター農業試験場

affil-num=5
en-affil=
kn-affil=岡山県農業総合センター農業試験場

affil-num=6
en-affil=
kn-affil=岡山県農業総合センター農業試験場

affil-num=7
en-affil=
kn-affil=岡山県農業総合センター農業試験場

affil-num=8
en-affil=
kn-affil=山梨県果樹試験場

affil-num=9
en-affil=
kn-affil=山梨県果樹試験場

affil-num=10
en-affil=
kn-affil=長野県中信農業試験場

affil-num=11
en-affil=
kn-affil=長野県中信農業試験場

affil-num=12
en-affil=
kn-affil=福岡県農業総合試験場
en-keyword=budbreak
kn-keyword=budbreak
en-keyword=chilling exposure
kn-keyword=chilling exposure
en-keyword=growing region
kn-keyword=growing region
en-keyword=temperature condition
kn-keyword=temperature condition
en-keyword=Vitis labrusca × V. vinifera
kn-keyword=Vitis labrusca × V. vinifera
END

start-ver=1.4
cd-journal=joma
no-vol=98
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=7
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Expression, Purification and Properties of Alanine Racemase from Thermus thermophillus HB8
kn-title=高度好熱性細菌 Thermus thermophilus HB8 由来アラニンラセマーゼの大腸菌での発現，精製及び諸性質の検討
en-subtitle=
kn-subtitle=
en-abstract=高度好熱性細菌 Thermus thermophilus HB8 由来アラニンラセマーゼ遺伝子を大腸菌中にクローニングし、発現させた後に、精製及び性質検討を行った。alr遺伝子は1080bpからなり360アミノ酸残基 HB8をコードしていたので、本酵素は38,596Daの分子量であると予想された。alr遺伝子の [ G + C ] 含量は、72%であり、Tm値は98.8℃であった。T.thermophilus HB8由来アラニンセマーゼを中等度好熱性細菌 Geobacillus stearothermophilus 及び赤痢菌 Shigella sonnei 由来アラニンラセマーゼと一次配列の比較をしたところ、G.stearothermophilus 由来のものと33%、赤痢菌由来の酵素を28%の相同性を示した。T.thermophilus HB8 由来アラニンラセマーゼを、70℃で10分間の熱処理後、DEAE-トーヨーパール陰イオン交換カラム等により精製した。精製酵素の最適温度は、d-アラニンからl-アラニンへの反応で55℃、l-アラニンからd-アラニンへの反応では60℃であり、最適pHは、9.0〜10.0であった。また、70℃で30分インキュベーションを行った後にも、活性の低下は見受けられず耐熱性を示した。更に、本酵素は分子量38,000モノマー酵素であると推定され、その反応機構に興味が持たれる。
kn-abstract=An alanine racemase (EC 5.1.1.1) from an extreme thermophilic bacterium Thermus thermophilus HB8, was purified and characterized, and its gene was cloned. The cloned alanine racemase gene (alr) was expressed in Escherichia coli JM 109. The alr gene is composed of a 1080 bp and encoded a 360 amino
acid, and was predicted to have a molecular weight of 38,596. The enzyme was purified by heat shock at 70°C for 10min and DEAE Toyopearl 650M column chromatography. The purified enzyme had an optimum pH9.0∼10.0 and an optimum temperature of 55°C∼60°C. Enzyme activity was retained 100% after incubation of the enzyme at 70°C for 10min. Alanine racemase from Thermus thermophilus HB8 is a monomeric enzyme with a molecular mass of 39 kDa.
en-copyright=
kn-copyright=

en-aut-name=YanagitaniMasahiko
en-aut-sei=Yanagitani
en-aut-mei=Masahiko
kn-aut-name=柳谷昌彦
kn-aut-sei=柳谷
kn-aut-mei=昌彦
aut-affil-num=1
ORCID=

en-aut-name=UemaeSatoshi
en-aut-sei=Uemae
en-aut-mei=Satoshi
kn-aut-name=上前智
kn-aut-sei=上前
kn-aut-mei=智
aut-affil-num=2
ORCID=

en-aut-name=ShiragaTomoyuki
en-aut-sei=Shiraga
en-aut-mei=Tomoyuki
kn-aut-name=白神智行
kn-aut-sei=白神
kn-aut-mei=智行
aut-affil-num=3
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=4
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学

affil-num=5
en-affil=
kn-affil=岡山大学
en-keyword=alanine racemase
kn-keyword=alanine racemase
en-keyword=pyridoxal 5’-phosphate
kn-keyword=pyridoxal 5’-phosphate
en-keyword=thermostable enzyme
kn-keyword=thermostable enzyme
en-keyword=Thermus thermophilus HB8
kn-keyword=Thermus thermophilus HB8
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=83
end-page=86
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Establishment of Science for Integrated Pest Management
kn-title=総合的害虫管理学の確立に向けて
en-subtitle=
kn-subtitle=
en-abstract=最近農業現場で IPM という英略語の知名度が高まっている．総合的害虫管理，または総合的病害虫・雑草管理（integrated pest management，以下 IPM と言う）のことであり，私がライフワークとしてきた分野である．岡山大学農学部または他大学での集中講義で，私の「総合的害虫管理学」の授業を受けて卒業し，国や県の農業職に就いた学生諸君と顔を合わせた時，在学中は良く理解出来なかったが，農業現場に来て IPM の重要性を認識出来たと言われることがしばしばある．農業現場では，IPM は持続的農業環境保全型農業における植物保護技術の切り札のように言われているが，果たしてそうであろうか．ここでは，IPM がどのような考え方に基づいているのかについて解説する（中筋房夫（1997）「総合的害虫管理学」養賢堂に詳しい)．
kn-abstract=Since the 1940's, we have had a powerful tool to combat
crop pests, i.e. synthetic pesticides. The application of pesticides has undoubtedly contributed to a remarkable
increase and stability in crop production. The heavy use of
unsuitable pesticides, however, has brought about various
undesirable effects on the agroecosystem and environment.
Frequent applications of these pesticides have led to the
development of resistance in key pests, and induced a resurgence of primary pests or an upsurge in secondary pests. In order to cope with the issue brought about by almost singular dependence on insecticidal control, the Food and Agriculture Organization’s (FAO) panel of experts proposed a new concept for future pest control, in 1965 as follows: “a pest management system that in the context of the associated environment and the population dynamics of the pest species, utilizes all suitable techniques and methods in a manner as compatible as possible and maintains pest population at levels below those causing economic injury”. The proposal has been called Integrated Pest Management (IPM) since the late 1970s. The proposal includes three key concepts, namely (1) integration of plural control measures, (2) economic injury level (EIL), and (3) population management system. Basecally, IPM integrates fundamental tactics, mainly natural control agents and subsidiary tactics, e.g. agricultural chemicals and physical methods. It is primarily significant in IPM that when both set of tactics are integrated, the latter tactics should not suppress the action of the former. Therefore any agricultural chemicals including pesticides must be highly selective against targeted pests, taking into account the crops, natural enemies and the environment. The concept of EIL suggests that the pest
population is not necessarily suppressed completely in IPM
and control should be implemented with due consideration
to a balance of cost and benefit. The population management
system is used for monitoring pest population dynamics to forecast whether or not the pest density will overshoot
an EIL. It is also used for assessing the efficacy of control tactics.
en-copyright=
kn-copyright=

en-aut-name=NakasujiFusao
en-aut-sei=Nakasuji
en-aut-mei=Fusao
kn-aut-name=中筋房夫
kn-aut-sei=中筋
kn-aut-mei=房夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=integrated pest management
kn-keyword=integrated pest management
en-keyword=synthetic insecticide
kn-keyword=synthetic insecticide
en-keyword=integration of plural control measures
kn-keyword=integration of plural control measures
en-keyword=economic injury level
kn-keyword=economic injury level
en-keyword=population management system
kn-keyword=population management system
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=69
end-page=81
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Vitis Coignetiae Grapes― Vine Physiology and Fruit Constituents ―
kn-title=ヤマブドウ研究― 樹及び果実の特性 ―
en-subtitle=
kn-subtitle=
en-abstract=岡山県真庭市蒜山で，地域特産物として1980年代から栽培が始められたヤマブドウ（Vitis coignetiae Pulliat）について，1993年から現在で，系統の識別，結実と果実発育の特性，果実成分の分析を行った．その結果，雌雄異株であるヤマブドウの結実安定のためには，受粉昆虫の活動が必要で，有用な数種の訪花昆虫が同定された．ヤマブドウの雌ずいは形態的に発育がよく，確実に受粉されれば，結実安定は達成される．しかし，ヤマブドウ樹は乾燥には強いが，耐湿性は低く，また，ベト病抵抗性も高くないので，ブドウ園の立地条件が重要である．成熟果実には糖，酸が高濃度で含まれるが，アミノ酸濃度は低い．しかし，探索の結果，一般の赤ワイン用品種
と同程度のアミノ酸含量を持つ樹が発見された．また，ヤマブドウ果実には高濃度の色素が含まれるが，同時にこの色素の光分解を抑制する成分が含まれていることが明らかになった．今後，この成分の同定と食品添加物としての利用が期待される．蒜山地区内のひるぜんワイン㈲ではヤマブドウワイン製造の技術改善が続けられ，赤およびロゼワインとして高い評価を得るようになった．また，「おかやまバイオアクティブ」の開発プロジェクトとしてワインビネガーの試作も行われた．現在，発ガン抑制などの機能性研究も進められており，健康食品としての生産拡大が期待される．
kn-abstract=Vitis coignetiae Pulliat is one of the naturally growing grape vines in the Japanese Islands, of which
the fruit has been utilized as a healthy juice and wine. In Hiruzen Highlands, the vines have been cultivated
for wine making since the 1980's. We have studied the physiology of berry set and berry maturation
of the vines to improve the fruit production and fruit quality for last 14 seasons. V. coignetiae vines
are dioecious and need insect pollination, mainly by two species of Diptera, Eristalis tenax and Eristalis
cerealis, and one species of Hymenoptera, Ceratina japonica, indicating that mix planting with male
vines and reservation of such insects are recommended. Once pollen grains have pollinated onto the
stigma, most of them grow a pollen tube to penetrate into ovule tissue and finally reach the embryo sac
to complete ovule fertilization. There are several types of coignetiae vines in Hiruzen vineyards that have
different genetic backgrounds. RAPD analysis of 15 vines revealed that they can be divided into three
groups and two individuals. Coignetiae vines have been found to have a lower tolerance for water logging
than other cultivars, whereas they have moderate drought tolerance. Furthermore, coignetiae vines are
not very disease tolerant, especially toward downy mildew, indicating that growers must take sufficient
care over drainage and fungus control. V. coignetiae vines accumulate high levels of sugar and acid into
the flesh and anthocyanins in the berry skin when they reach the full ripe stage, which is mid and late
October in Hiruzen. Tartaric acid, the main acid constituent in coignetiae berries, is contained at levels
as high as 1.0%. Amino acid concentration in the juice is much lower in most coignetiae vines than other
wine grapes, although a special coignetiae vine with berries with high amino acid content has been found.
V. coignetiae berries have been proved to have several functional properties such as high free radical
scavenging activity and anti-photodecomposition of anthocyanin pigments. As commercial products of
coignetiae berries, beautiful wines, pure juice, and wine vinegar with a rich and fruity taste are produced.
en-copyright=
kn-copyright=

en-aut-name=OkamotoGoro
en-aut-sei=Okamoto
en-aut-mei=Goro
kn-aut-name=岡本五郎
kn-aut-sei=岡本
kn-aut-mei=五郎
aut-affil-num=1
ORCID=

en-aut-name=GotoShintaro
en-aut-sei=Goto
en-aut-mei=Shintaro
kn-aut-name=後藤信太郎
kn-aut-sei=後藤
kn-aut-mei=信太郎
aut-affil-num=2
ORCID=

en-aut-name=UekiKeiji
en-aut-sei=Ueki
en-aut-mei=Keiji
kn-aut-name=植木啓司
kn-aut-sei=植木
kn-aut-mei=啓司
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=ひるぜんワイン(有)
en-keyword=juice constituent
kn-keyword=juice constituent
en-keyword=skin anthocyanin
kn-keyword=skin anthocyanin
en-keyword=functional food
kn-keyword=functional food
en-keyword=Vitis coignetiae
kn-keyword=Vitis coignetiae
en-keyword=wine
kn-keyword=wine
en-keyword=wine vinegar
kn-keyword=wine vinegar
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=61
end-page=67
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Medium Shape, Fertilization Methods and Plant Density on the Growth of Pansy Grown in Medium Hardened by
Heat Fusion Polyester Fiber without Polyethylene Pot
kn-title=培地の形状，施肥方法，栽植密度が熱融着性ポリエステル繊維固化ポットレス培地で育てたパンジーの生育に及ぼす影響
en-subtitle=
kn-subtitle=
en-abstract=固化培地を利用したポリポットを用いないポットレス栽培において，潅水効率の上昇，施肥の簡易化，藻の発生抑制のため，培地の形状，施肥方法および栽植密度がパンジーの生育に及ぼす影響について検討した．9㎝角のポット型培地に6×6×深さ3㎝のくぼみ（ウォータースペース）を培地上部に作成すると，栽培前期の水供給量（灌水後の重量―灌水前の重量）が約10～25% に多くなった．しかし，栽培中期から後期にかけてはその違いは次第に小さくなり，特に曇雨天時には水供給量に差はみられなくなった．ウォータースペースの有無にかかわらず，ポリポットがあると生育に違いは認めらなかったが，ポットレス区ではウォータースペースによって生育が改善された．元肥量10ｇ/liter 区では生育初期に高濃度障害と思われる症状が見られ，5ｇ/liter 区より生育が抑制された．栽植密度を半分にしたスペーシングによって，草丈はかなり抑制された．スペーシングなしの場合にはポットの有無による差は小さかったが，ポットレス栽培の生育がスペーシング栽培でかなり改善された．ポットレス・スペーシング有・灌水量120ml区の生育が最も旺盛であった．養分量が多いほど灌水量が多いほど，藻の発生が大であったが，スペーシングとポットレスを組み合わせると藻の発生が改善された．
kn-abstract=To develop bedding plant production system without polyethylene pots (PP), problems that may be
encountered with compacted medium hardened by heat fusion polyester fiber were investigated. Effects
of medium shape, fertilization methods and plant density on the growth of pansy in medium without
PP were investigated. To improve water usage on compacted medium, a watering space (6×6×depth
3cm) was made on the upper part of the compacted medium (9×9×depth 9cm). In the early stages of
the experiment the amount of water supplied per pot (weight after irrigation-weight before irrigation)
of medium with water space was 10–25% greater than that of medium without a water space. However,
from the mid to late stages of the experiment, the difference gradually declined. The amount of water
supplied per pot was similar between with and without water space especially on cloudy or rainy days.
The growth of pansies on medium without PP was remarkably greater with water space than without
water space. On 10g/liter basal fertilizer treatment, pansies had severe symptoms of fertilizer damage.
The growth of pansy on 10g/liter basal fertilizer was inhibited compared to that of 5g/liter basal fertilizer.
Spacing treatment was conducted whenever plant density was half. Height of pansy grown with
spacing treatment was significantly lower than that without spacing treatment. PP had no effects on
growth when pansy was grown without spacing treatment. However, when pansy was grown with spacing
treatment, pansy grown without PP had significantly greater growth than with PP. Algal growth on
the medium increased with increasing irrigation rate or nutrient rate. Combination of spacing treatment
and medium without PP decreased the rate of algal growth on medium.
en-copyright=
kn-copyright=

en-aut-name=GotoTanjuro
en-aut-sei=Goto
en-aut-mei=Tanjuro
kn-aut-name=後藤丹十郎
kn-aut-sei=後藤
kn-aut-mei=丹十郎
aut-affil-num=1
ORCID=

en-aut-name=OohashiYuji
en-aut-sei=Oohashi
en-aut-mei=Yuji
kn-aut-name=大橋佑司
kn-aut-sei=大橋
kn-aut-mei=佑司
aut-affil-num=2
ORCID=

en-aut-name=ShimizuNozomi
en-aut-sei=Shimizu
en-aut-mei=Nozomi
kn-aut-name=清水希
kn-aut-sei=清水
kn-aut-mei=希
aut-affil-num=3
ORCID=

en-aut-name=MorisitaTeruhisa
en-aut-sei=Morisita
en-aut-mei=Teruhisa
kn-aut-name=森下照久
kn-aut-sei=森下
kn-aut-mei=照久
aut-affil-num=4
ORCID=

en-aut-name=FujiiKazunori
en-aut-sei=Fujii
en-aut-mei=Kazunori
kn-aut-name=藤井一徳
kn-aut-sei=藤井
kn-aut-mei=一徳
aut-affil-num=5
ORCID=

en-aut-name=IshikawaJunya
en-aut-sei=Ishikawa
en-aut-mei=Junya
kn-aut-name=石川順也
kn-aut-sei=石川
kn-aut-mei=順也
aut-affil-num=6
ORCID=

en-aut-name=ShimaKohji
en-aut-sei=Shima
en-aut-mei=Kohji
kn-aut-name=島浩二
kn-aut-sei=島
kn-aut-mei=浩二
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=みのる産業(株)植物工学研究所

affil-num=5
en-affil=
kn-affil=みのる産業(株)植物工学研究所

affil-num=6
en-affil=
kn-affil=兵庫県立農林水産技術総合センター

affil-num=7
en-affil=
kn-affil=和歌山県農林水産総合技術センター農業試験場
en-keyword=algal
kn-keyword=algal
en-keyword=bedding plant
kn-keyword=bedding plant
en-keyword=spacing
kn-keyword=spacing
en-keyword=water usage
kn-keyword=water usage
en-keyword=watering space
kn-keyword=watering space
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=55
end-page=59
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Production of Full Ripe and Aromatic Muscat of Alexandria
Grapes and the Preferences of Consumers
kn-title=濃厚・完熟マスカットの生産と消費者の嗜好性
en-subtitle=
kn-subtitle=
en-abstract=マスカットを根域制限ベッドで栽培し，成熟開始期から適度の水分ストレスを与えて生産した完熟・濃厚マスカットを，果実店などを通じて消費者に販売し，その嗜好性を調査した．2006年は主として関東圏の消費者が購し，このマスカットを「自然の姿で，味，香りともよい」という評価が多かった．しかし，2007年はほとんどが兵庫県内の消費者にわたり，「味はよいが，果粒は小さく，種が多く，食べにくい」との感想が多かった．この
調査結果を基として，岡山における今後のマスカット生産技術の方向性について考察した．
kn-abstract=Full ripe Muscat clusters with high aroma, produced by root zone restriction and partial root drying
method, were sold to consumers in the seasons of 2006 and 2007. Their preferences concerning the
clusters were examined by obtaining their opinions through the questionnaire method. In the 2006 test,
most consumers, mainly living in the Kanto district, preferred clusters with a natural cluster shape and
strong taste and aroma, while in the 2007 test Kansai consumers commented that the berry size must be
bigger and the seeds should be removed, although the taste was acceptable. Such different responses
depending on the consumer district should be noted when discussing future strategies for Muscat of
Alexandria grape growing in Okayama.
en-copyright=
kn-copyright=

en-aut-name=OkamotoGoro
en-aut-sei=Okamoto
en-aut-mei=Goro
kn-aut-name=岡本五郎
kn-aut-sei=岡本
kn-aut-mei=五郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=Muscat of Alexandria grape
kn-keyword=Muscat of Alexandria grape
en-keyword=full ripe
kn-keyword=full ripe
en-keyword=aroma
kn-keyword=aroma
en-keyword=consumers
kn-keyword=consumers
en-keyword=preference
kn-keyword=preference
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=49
end-page=54
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Potential for Tomato Cultivation Using Capillary Wick- watering Method
kn-title=｢毛管給水ひも」によるトマト栽培の可能性について
en-subtitle=
kn-subtitle=
en-abstract=「毛管給水ひも」によるトマト栽培の可能性を探るため，中玉トマトの10段摘心栽培を試み，ひもの特性と栽培上の問題点を明らかにした．1/5,000ａワグネルポットの底から5㎜位と50㎜位に小穴を開け，そこへ毛管ひも（幅4㎝，長さ45㎝）の一端を導入し，そのまま鉢中央を横切り対壁に沿って土壌表面まで配置した．他の一端は培養液に浸した．この毛管ひもを遮根透水シートで被覆し（接着あるいは機械織りによるチューブ状管にひもを挿入），その「被覆ひも」を「無被覆ひも」と比較したところ，被覆ひもでは根の侵入が強く抑制でき成育が旺盛で収量も高かった．被覆しないと1か月後には根が毛管ひも内に伸長して貯液槽にまで達した．この根を除去すると多くの個体は萎れの症状を呈した．この症状は50㎜位の方が著しかった．被覆が不完全だと接着部あるいは織り込み部から根は毛管ひもに侵入する．従って，毛管ひもは完全被覆されることが重要であるといえる．また，遮根透水ひもを2㎝幅，4㎝幅としシングル状とダブル状で比較したところ，成育半ばでシングル2㎝幅では萎れ症状が観察され枯死する個体も現れた．その
他では成育等に大きな差異は認められなかったが，いずれの処理区においても多くの尻腐れ果が発生した．トマトの吸水に伴って水位は低下するため本試験では毎日，培養液を手で補給した．しかし，水位変化は最大で3㎝と大きかった．尻腐れ果発生はこの液槽の水位レベル管理に起因するところ大と推察され，今後の課題として残された．
kn-abstract=This research was conducted to investigate any potential problems that may be encountered while
using capillary wick irrigation system. Medium-fruited tomato plants were cultivated in spring up to the
10th truss.
In the first experiment, a 1/5,000a wagner pot was used, and small openings were made 5mm or
50mm from the bottom of the pots. Capillary wicks, 4 cm in width and 45 cm in length were inserted
into the slits and aligned to the pot’s inner wall perpendicularly to the soil surface. The other end of the
wick was dipped in half or full strength Ohtsuka-A nutrient solution. The wick was covered with water
permeable root-barrier material to prevent root penetration into wick. This resulted in good growth and
relatively high yield. Without covering, roots grew vigorously into the wicks and 1 month after transplanting
extended to the reservoir solution. Removal of root overgrowth caused wilting in some of the
plants. This symptom was more pronounced where the wick insertion points were 50 mm from the base.
Root contexture was observed in non-covered and imperfectly covered wicks. These results indicate that
it is important to make a wick with a perfect covering for stable plant growth and fruit yield.
In the second experiment, the capacity of 2 cm or 4 cm wide with and single or double layers of capillary
wicks to transport water to the plant root-zone was examined. Some of the plants wilted in the 2 cm
single layer, while no differences were observed in other treatments. Furthermore, a high percentage of
blossom-end rot was recorded in all treatments. In response to water uptake by the plants, the nutrient
solution was supplied daily depending on the decrease of water in the reservoir, in order to maintain the
water level fluctuation within a 3 cm range. This fluctuation may account for the blossom-end rot
prevalence observed, probably due to water-stress imposed on the plants by unstable water level in the reservoir.
en-copyright=
kn-copyright=

en-aut-name=MasudaMasaharu
en-aut-sei=Masuda
en-aut-mei=Masaharu
kn-aut-name=桝田正治
kn-aut-sei=桝田
kn-aut-mei=正治
aut-affil-num=1
ORCID=

en-aut-name=FukumotoShoko
en-aut-sei=Fukumoto
en-aut-mei=Shoko
kn-aut-name=福元祥子
kn-aut-sei=福元
kn-aut-mei=祥子
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=capillary wick
kn-keyword=capillary wick
en-keyword=root-proof cover
kn-keyword=root-proof cover
en-keyword=watering method
kn-keyword=watering method
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=41
end-page=47
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ブドウの芽の休眠打破に伴う生理的変化
kn-title=Physiological Aspects of Bud Associated with　Breaking Dormancy in Grapevines
en-subtitle=
kn-subtitle=
en-abstract=ブドウ‘ピオーネ’について，休眠の導入と覚醒の過程における芽の生理的変化を調査した．休眠導入期の8月から覚醒初期の12月まで露地で栽培されている個体から芽を採取し，呼吸量，エチレン生成量および含水率を測定し
た．調査期間を通して呼吸量は低く，エチレンもほとんど検出されなかった．芽の含水率は8月から10月まで僅かに上昇し，その後は変化がみられなかった．休眠覚醒初期の12月から発芽期の4月中旬まで，芽の呼吸量とエチレン生成量を測定した．呼吸量は4月上旬までは低く推移し，発芽（4月13日）の直前に急上昇した．エチレンは測定期間を通して低かった．休眠期の12月，1月および2月に採取した穂木を2% H2CN2 または蒸留水（対照）で処理し，25℃以上に保ったプラスチックハウスに入れて発芽を調査するとともに，経時的に芽の呼吸量，エチレン生成量および含水率を測定した．両時期とも対照区よりも H2CN2 処理区の発芽が早く，しかも休眠の深い12月処理で区による差が大きかった．両区いずれの時期とも，芽の呼吸量は発芽直前に急上昇したのに対し，エチレン生成量は調査期間を通して低いままであった．芽の含水率は，いずれの時期および処理区とも発芽期に低下し，特に H2CN2 処理区の低下が大きかった．休眠最深期の10月に採取した穂木に ACC，GSH（還元型グルタチオン）および GSSG（酸化型グルタチオン）を処理し，発芽に及ぼす影響を調査したところ，ACC だけが発芽を促進した．同様に，4種のシアン化合物（CaCN2，H2CN2，KCN，NaCN）を処理したところ，H2CN2を除き有意に発芽を促した．これらの結果を基に，ブドウの発芽と生理的変化との関係および休眠覚醒に及ぼすエチレン生合成関連物質の作用性について考察した．
kn-abstract=Changes in CO2 and C2H4 production and water content of bud associated with breaking in
‘Pione’ grapevine (Vitis labrusca ×V. vinifera) were investigated throughout dormancy. Buds
were collected monthly from August to December, during dormancy induction and maintenance,
and CO2 and C2H4 production were determined by GC after incubation. Both CO2 and
C2H4 production, especially for the latter, were low throughout the experiment. Water content of
bud gradually increased until October ; thereafter it was constant. When CO2 and C2H4 production
was determined from December to April, during dormancy maintenance to release, CO2
production was low from beginning of experiment to early April, prior to bursting, then rapidly
increased to April 13, the bursting date. C2H4 production was almost undetectable throughout
the experiment. Cuttings obtained at 3 different stages of dormancy were applied with 2%
H2CN2 or distilled water (control), and budbreak was monitored in a plastic house kept at 20℃
or more. The CO2 and C2H4 production of bud were also determined weekly until budbreak.
Regardless of treatment time H2CN2 significantly promoted budbreak compared to the control.
Significantly higher production of CO2 was observed in cuttings treated with H2CN2 at 3 to 9
days before bursting for all the treatment times. C2H4 production was very low throughout the
experiment for all the treatments. Irrespective of chemical application and treatment time, water
content of bud decreased to the bursting stage, H2CN2 treatment especially showing a large
decline. When dormant cuttings were treated with ACC, GSH (reduced glutathione) and GSSG
(oxidized glutathione), only ACC promoted budbreak. Budbreak in cuttings treated with cyanamides
such as CaCN2 and H2CN2 and cyanides such as KCN and NaCN was significantly accelerated
except for H2CN2. Based on these results, the relationship between budbreak of grapevine
buds and physiological changes in buds, and the roles of substances related to ethylene biosynthesis
on breaking bud dormancy are discussed.
en-copyright=
kn-copyright=

en-aut-name=PotjanapimonChaiwat
en-aut-sei=Potjanapimon
en-aut-mei=Chaiwat
kn-aut-name=ポジャナピモンチャイワット
kn-aut-sei=ポジャナピモン
kn-aut-mei=チャイワット
aut-affil-num=1
ORCID=

en-aut-name=FujiokaNatsuko
en-aut-sei=Fujioka
en-aut-mei=Natsuko
kn-aut-name=藤岡奈津子
kn-aut-sei=藤岡
kn-aut-mei=奈津子
aut-affil-num=2
ORCID=

en-aut-name=FukudaFumio
en-aut-sei=Fukuda
en-aut-mei=Fumio
kn-aut-name=福田文夫
kn-aut-sei=福田
kn-aut-mei=文夫
aut-affil-num=3
ORCID=

en-aut-name=KubotaNaohiro
en-aut-sei=Kubota
en-aut-mei=Naohiro
kn-aut-name=久保田尚浩
kn-aut-sei=久保田
kn-aut-mei=尚浩
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=grapevine
kn-keyword=grapevine
en-keyword=breaking bud dormancy
kn-keyword=breaking bud dormancy
en-keyword=iration rate
kn-keyword=iration rate
en-keyword=ethylene production
kn-keyword=ethylene production
en-keyword=substances related to ethylene biosynthesis
kn-keyword=substances related to ethylene biosynthesis
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=33
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of Rising Temperature on Grain Quality and Palatability of Rice Cultivars
kn-title=気温上昇が水稲品種の玄米外観品質，食味におよぼす影響
en-subtitle=
kn-subtitle=
en-abstract=岡山県平野部の主要水稲品種は，中生のヒノヒカリ，晩生のアケボノである．近年，夏期の高温障害が原因とされる乳白米，腹白米などのいわゆる白未熟粒の発生による玄米外観品質の低下が顕著となっており，その回避技術やより高品質，良食味品種の選択など，さまざまな対策が検討されている．このような背景のもと，1996年に九州地方において多収，良食味の観点から「にこまる」，「あきまさり」が育成された．にこまるはヒノヒカリと同等の熟期で極良食味であることに加えて，玄米外観品質が優れ，高温下における白未熟粒の発生が少ない品種である．一方あきまさりは，アケボノと同等の熟期で，ヒノヒカリ並みの食味を有する品種である．すなわち，これら２品種をヒノヒカリ，アケボノに替わる品種として導入することにより，より良食味で高品質米の生産が可能であると考えられる．著者らは，水田および畑圃場に温度勾配チャンバー（TGC）を設置し，温暖化による気温上昇を想定した作物（水稲，ダイズ，冬コムギ）の生産性を評価してきた．水稲（品種：日本晴）では，気温上昇によって不稔籾の増加することで減収となること，白未熟粒の発生によって玄米外観品質が低下することを報告した．また，気温上昇下での米飯の食味についても検討したが，その品種間差については検討を行っていない．本研究では，水田内に設置した TGC を用いて，移植から収穫に至る生育期間全体の気温上昇が，水稲４品種の収量，玄米外観品質，米飯の食味におよぼす影響の品種間差異を検討した．
kn-abstract=The effects of high temperatures on grain quality and palatability of cooked rice of four rice cultivars were examined in a temperature gradient chamber (TGC). Experimental plots going from TG1 (near the air intake side) to TG3 (near the air exhaust side) along the temperature gradient in TGC, corresponding to low and high temperature, and an open field plot (outside of TGC) were arranged. The mean and maximum air-temperatures in TG3 were 3.6℃ and 7.2℃ higher, respectively, than those in TG1. Brown rice yield per m2 in the outside was the highest, and those in the TGC plots were lower than outside in all cultivars. This yield decline was due to the decrease in the percentage of ripened spikelets. The
increase in the percentage of sterile spikelets due to high temperatures in Nikomaru and Akimasari was larger than that in Hinohikari and Akebono. The yield difference among TGC plots was not significant. High temperature increased the white berry grains and white back grains in Hinohikari and Akebono, respectively. The overall eating quality (＋3 to －3) in the outside was in the order of Nikomaru (0.56)
＞Akimasari (0.50)＞Hinohikari (0.00)＞Akebono (－0.06). The overall eating quality in TG3 was lower
than outside, especially in the quality of appearance. However, the decreasing degrees of appearance quality in Nikomaru and Akimasari were smaller than those in Hinohikari and Akebono. The protein content of milled rice in TG3 (6.0−7.8%) was higher than that in the outside (8.6−10.4%) in all cultivars. The increase in protein content by higher temperature was in the order of Akebono (4.4%)＞Hinohikari (1.9%)＝Akimasari (1.9%)＝Nikomaru (0.8%). In terms of grain quality, the cultivation of new cultivars, Nikomaru and Akimasari are recommended when air temperature exceeds normal years. However, yield reductions in Nikomaru and Akimasari caused by high temperatures were larger than those in the conventional cultivars, Hinohikari and Akebono.
en-copyright=
kn-copyright=

en-aut-name=Oh-eIzumi
en-aut-sei=Oh-e
en-aut-mei=Izumi
kn-aut-name=大江和泉
kn-aut-sei=大江
kn-aut-mei=和泉
aut-affil-num=1
ORCID=

en-aut-name=KobayashiYasuko
en-aut-sei=Kobayashi
en-aut-mei=Yasuko
kn-aut-name=小林恭子
kn-aut-sei=小林
kn-aut-mei=恭子
aut-affil-num=2
ORCID=

en-aut-name=SaitohKuniyuki
en-aut-sei=Saitoh
en-aut-mei=Kuniyuki
kn-aut-name=齊藤邦行
kn-aut-sei=齊藤
kn-aut-mei=邦行
aut-affil-num=3
ORCID=

en-aut-name=KurodaToshiro
en-aut-sei=Kuroda
en-aut-mei=Toshiro
kn-aut-name=黒田俊郎
kn-aut-sei=黒田
kn-aut-mei=俊郎
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=岡山大学

affil-num=4
en-affil=
kn-affil=岡山大学
en-keyword=Grain quality
kn-keyword=Grain quality
en-keyword=High temperature
kn-keyword=High temperature
en-keyword=Palatability
kn-keyword=Palatability
en-keyword=Rice (Oryza sativa L.)
kn-keyword=Rice (Oryza sativa L.)
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=25
end-page=31
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=異数体を含むレンゲ人為同質４倍体集団での全兄弟と半兄弟の共分散の計算
kn-title=Calculation of Full and Half Sib Covariances in an Artificial Autotetraploid Population Including Aneuploids, in Astragalus Sinica L.
en-subtitle=
kn-subtitle=
en-abstract=任意交配するレンゲ人為同質４倍体集団における全兄弟と半兄弟の共分散を計算した．特定の相同染色体が必ずしも異数体に関わるとは限らないので，特定の相同染色体が関わる場合と関わらない場合について共分散を計算し，平均しなければならない．共分散を平均するため，特定の相同染色体が異数性に関わる確率を３/８とした“８”と“３”はゲノム染色体数と正４倍体で形成される４価染色体数の平均値である．４価染色体は MI で確率κ＝ 0.8とλ＝ 0.2（κ＋λ＝１）で２-２と１-３に分配され，Ⅲ価染色体とⅤ価染色体は確率１で１-２と２-３に分配されるとし，２xと２x＋１花粉と雌性配偶子は等しく受精するとして共分散を計算した．両親の近交系数はＦ＝０であると仮定した．次いで家族の共分散を家族内の兄弟間の共分散の平均として計算し，集団の共分散を家族の共分散の平均として計算した．仮定に基づき求めた共分散の分散成分の係数は２x花粉のみが受精するとして計算した値と違っていた．相加遺伝分散成分の係数は全兄弟と半兄弟でそれぞれ3.3％と7.2％ずつ違っていた．他の分散成分も同様であった．実際のレンゲ人為同質４倍体集団では２x＋１花粉は受精能力が２x花粉より低く稀にしか受精しないので，２x花粉のみが受精するとして全兄弟と半兄弟の共分散を計算しても問題はないであろう．
kn-abstract=Full and half sib covariances were investigated in an artificial autotetraploid population with
random mating in Astragalus sinicus L.. Since a set of homologous chromosomes is not necessarily
involved in aneuploidy, the covariances must be averaged for two cases, that is, with and
without involvement. To average the covariances, the probability that a set of homologous chromosomes
was involved in aneuploidy was assumed as 3/8, where “8” and “3” represent the
chromosome number of a genome and the mean number of quadrivalent chromosomes formed
in a euploid, respectively. The covariances were calculated under the assumption that quadrivalent
chromosomes were distributed to the poles by 2-2 and 1-3 with probabilities κ＝ 0.8 and λ
＝0.2 (κ＋λ＝1) respectively, and that trisomic and pentasomic chromosomes were distributed
by 1-2 and 2-3 both with a probability of 1. It was also assumed that the inbreeding coefficient
of the parents was F＝ 0, and that 2x and 2x＋ 1 pollens and all female gametes could fertilize
equally. The covariance of a family was taken as an average of the covariance of each sib combination
in a family. As a result, the covariance of a population could be obtained as an average of
the covariance of each family in a population. The coefficients of variance components calculated
under these assumptions were different from those calculated under the same condition except
that 2x＋ 1 pollen could not fertilize. Differences in the coefficient of additive genetic variance
components were about 3.3% and 7.2% for full and half sib covariances, respectively.
Coefficients of the other variance components were also different between the two cases.
However, 2x＋1 pollen could rarely fertilize, since their ability to fertilize in a practical population
were lower than 2x pollen. Therefore, it would be valid to calculate full and half sib covariances
in an artificial autotetraploid population of Astragalus sinicus L. under the condition
thatonly 2x pollen could fertilize.
en-copyright=
kn-copyright=

en-aut-name=MorisawaTetsuo
en-aut-sei=Morisawa
en-aut-mei=Tetsuo
kn-aut-name=森澤徹男
kn-aut-sei=森澤
kn-aut-mei=徹男
aut-affil-num=1
ORCID=

en-aut-name=KatoKenji
en-aut-sei=Kato
en-aut-mei=Kenji
kn-aut-name=加藤鎌司
kn-aut-sei=加藤
kn-aut-mei=鎌司
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=高知県立安芸高等学校

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=full and half sib covariances
kn-keyword=full and half sib covariances
en-keyword=quadrivalent chromosomes
kn-keyword=quadrivalent chromosomes
en-keyword=additive genetic variance
kn-keyword=additive genetic variance
en-keyword=variance of a family
kn-keyword=variance of a family
en-keyword=covariance of a population
kn-keyword=covariance of a population
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=17
end-page=24
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=人為同質４倍体集団における全兄弟及び半兄弟共分散の数学モデル
kn-title=Mathematical Model for the Calculation of Full and
Half Sib Covariance in an Artificial Autotetraploid
Population Including Aneuploids
en-subtitle=
kn-subtitle=
en-abstract=人為同質４倍体集団の遺伝分散を求めるため，両親が近交系数Ｆ＝０の同質４倍体家族の全兄弟と半兄弟の共分散を検討した．Kempthorne のモデルにおいて定義された分散の係数を①Ａ，Ｄ，Ｔ，Ｑと②φ，ψの２つに分割し
た．①は互いに独立な相加，２遺伝子，３遺伝子，４遺伝子効果の組み合わせの確率である．②は兄弟が片親から受け取る同一対立遺伝子の数と対立遺伝子ペアの数である．これは兄弟が片親から対立遺伝子と対立遺伝子の組を受け取る確率の関数であり，この確率は減数分裂での染色体行動と配偶子の染色体数によって決まる.この確率を推定するため，Ⅳ価染色体は確率κ，λ（κ＋λ＝１）で２-２と１-３で分配され，Ⅲ価染色体とⅤ価染色体は１-２と２-３に分配されると仮定した．本報告では，四染色体が完全にⅣ価染色体を形成するとして，全ての雌雄の配偶子がその染色体数に関係なく受精できる単純な場合について検討した．共分散の分散成分の構造は兄弟の組み合わせと家族によって異なる．したがって，家族の共分散は各兄弟の共分散
とその組み合わせ頻度を用い平均すれば求めることができ，集団の平均の共分散は家族の共分散と集団での家族の頻度を用い平均すれば求めることができる．
kn-abstract=For the estimation of genetic variance of an artificial autotetraploid population, a mathematical
model of full and half sib covariances between sibs with various chromosome numbers,
which were derived from euploid or aneuploid parents, was devised for a case where the
inbreeding coefficient of the parents was F＝0. The coefficients defined in Kempthorne's model
were separated into two parts: (i) A, D, T and Q, and (ii) φ and ψ. The former four parameters
were defined as probabilities of factor combinations, which could be compared between various
sibs, for additive, digenic, trigenic, and quadrigenic effects, and were mutually independent. The
latter two parameters, which were the numbers of the identical allele and the identical allele pair
combinations that two sibs inherited from a parent, were defined as linear functions of the probabilities
that two sibs inherited allele or allele pair from a parent, respectively. These probabilities
depend on chromosome behavior during meiosis and the chromosome number of the gametes.
For the estimation, it was assumed that quadrivalent chromosomes were distributed by 2-2
and 1-3 with probabilities κ and λ (κ＋λ＝ 1), respectively. The distribution of trisomic and
pentasomic chromosomes to the poles was assumed to be 1-2 and 2-3. Then, the probabilities
were estimated for the simple case where all male and female gametes could equally fertilize
irrespective of their chromosome number, provided that tetrasomic chromosomes completely
formed a quadrivalent chromosome.
　The constitution of variance components were different according to the sib combinations and
family. Therefore, for the calculation of the covariance of a family, the covariances between
various sibs were averaged by the combination frequency in a family, and for the calculation of
the covariance of population, the family's covariances were averaged by the family's frequency in the population.
en-copyright=
kn-copyright=

en-aut-name=MorisawaTetsuo
en-aut-sei=Morisawa
en-aut-mei=Tetsuo
kn-aut-name=森澤徹男
kn-aut-sei=森澤
kn-aut-mei=徹男
aut-affil-num=1
ORCID=

en-aut-name=KatoKenji
en-aut-sei=Kato
en-aut-mei=Kenji
kn-aut-name=加藤鎌司
kn-aut-sei=加藤
kn-aut-mei=鎌司
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=高知県立安芸高等学校

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=artificial autotetraploid
kn-keyword=artificial autotetraploid
en-keyword=covariance
kn-keyword=covariance
en-keyword=variance component
kn-keyword=variance component
en-keyword=euploid
kn-keyword=euploid
en-keyword=aneuploid
kn-keyword=aneuploid
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=15
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ハツカダイコンの胚軸肥大成長に対するトリフルオロインドール酪酸の効果
kn-title=Effects of a Plant Growth Regulator 4,4,4–Trifluoro–3–(indole–3–)–butyric Acid on Thickening Growth of Radish Hypocotyl
en-subtitle=
kn-subtitle=
en-abstract=ハツカダイコンの肥大成長に対する合成植物成長調節物質トリフロオロインドール酪酸（TFIBA）の効果について調べた．種子を10－4ないしは10-5M TFIBA 溶液に24時間浸漬し，発芽種子をバーミキュライトの入ったポットに移植して，14時間日長，昼夜温度23℃/18℃のグロスチャンバーで栽培すると，明らかな胚軸の肥大成長促進が認められた．肥大成長は10－4M TFIBA 処理の植物体でより顕著に現れた．同様に処理した発芽種子を自然光，無加温のガラス室で，2007年３月から４月にかけて栽培すると，胚軸の肥大成長はグロスチャンバーでの栽培に比べて，より顕著に現れた．35日目の肥大胚軸部の新鮮重は，10－4Ｍと10－5Ｍ のいずれの濃度においても30ﾝ50ｵ増加した．TFIBA処理によって生育初期段階で胚軸が20ﾝ50ｵ長くなり，また葉数が増えて地上部重が有意に重くなり，胚軸の肥大部径も有意に大きくなった．これらの結果は，TFIBA が胚軸の初期成長を促進し，生育の進行と共に光合成器官である葉組織を増加させ，胚軸の肥大成長を促進したことを示している．可食部である胚軸肥大部の形状は，ガラス室ではきれいな球状となるものが多いのに比べて，グロスチャンバーでは縦長のいびつな形をしたものが多くなった．これは，グロスチャンバーでは光量が不足し，十分な光合成産物が胚軸の肥大に供給されなかったためと考えられた．
本研究で，TFIBA を発芽時に浸漬投与するだけでハツカダイコンの可食部（胚軸）の肥大成長を有意に促進させることが明らかとなった．得られた結果は，TFIBA がハツカダイコンをはじめとする根茎野菜の生産促進に有効な植物成長調節物質であることを示している．
kn-abstract=Effects of a synthesized plant growth regulator 4,4,4–trifluoro–3–(indole–3–)–butyric acid
(TFIBA) on thickening growth of radish hypocotyls were investigated. The thickening growth of
radish hypocotyls, which are the edible part, was stimulated by TFIBA applied to the seeds before
transplanting. The fresh weight of the hypocotyls in the seedlings treated with 10－4mol L－1
(10－4M) TFIBA solution significantly increased during cultivation in a growth chamber for 28
days as compared to that in the control. However, such significant increase was not observed in
the seedlings treated with 10－5mol L－1 (10－5M) TFIBA solution. The fresh weight and maximal
diameter of hypocotyls in the seedlings treated with TFIBA solution of 10－4M and 10－5M were
significantly increased by 30–50 % as compared to those of the control at 35 days after transplanting
when the seedlings were cultured in a green house under natural light and temperature
conditions. The length of hypocotyls in the seedlings treated with 10－4M and 10－5M TFIBA
increased approximately 20–50 % during the early growth stage as compared to that in the control.
The fresh and dry weight of the shoots in the seedlings was apparently increased by treatment
with 10－4M and 10－5M TFIBA solutions at 35 days after transplanting. These results suggest
that TFIBA stimulates hypocotyl elongation and total photosynthetic activity in the shoots
and results in promotion of thickening growth of the hypocotyls.
en-copyright=
kn-copyright=

en-aut-name=LiXiuping
en-aut-sei=Li
en-aut-mei=Xiuping
kn-aut-name=李秀平
kn-aut-sei=李
kn-aut-mei=秀平
aut-affil-num=1
ORCID=

en-aut-name=SasakawaHideo
en-aut-sei=Sasakawa
en-aut-mei=Hideo
kn-aut-name=笹川英夫
kn-aut-sei=笹川
kn-aut-mei=英夫
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学
en-keyword=hypocotyls
kn-keyword=hypocotyls
en-keyword=plant growth regulator
kn-keyword=plant growth regulator
en-keyword=radish
kn-keyword=radish
en-keyword=thickening growth
kn-keyword=thickening growth
en-keyword=trifluoro-indole-butyric acid (TFIBA)
kn-keyword=trifluoro-indole-butyric acid (TFIBA)
END

start-ver=1.4
cd-journal=joma
no-vol=97
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=7
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=200802
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Purification, Characterization and Crystal Structure of Isoamylase from Thermophilic Bacteria Rhodothermus marinus
kn-title=海産性好熱性細菌 Rhodothermus marinus 由来イソアミラーゼ
の精製，性質検討及びＸ線結晶構造解析
en-subtitle=
kn-subtitle=
en-abstract=Rhodothermus marinus 由来イソアミラーゼ遺伝子を組み込んだプラスミド pBX２を使用し，大腸菌 Top10株を形質転換し，16時間の前培養，24時間の本培養後，菌体破砕し，得られた無細胞抽出液を熱処理（80℃，10 min），50ｵ硫安分画，陰イオン交換カラムクロマトグラフィー（DEAEﾝトヨパール），ハイドロキシアパタイトカラムクロマトグラフィーに供して本酵素の精製を行った．本精製酵素の性質検討を行った結果，本酵素の最適反応温度は70℃，pH４であり，また本酵素は60℃で１時間処理しても活性が低下することが無く，Pseudomonas amyloderamosa 由来イソアミラーゼよりも高い耐熱性を有することが判明した．本酵素の結晶化・X線結晶構造解析を行った結果，本酵素は P. amyloderamosa 由来イソアミラーゼと同様Ｎドメイン・ＡドメインＣドメインの３つのドメインから構成されており，活性残基（Ｄ359，Ｅ395，Ｄ467）など活性中心付近のアミノ酸残基も P. amyloderamosa 由来イソアミラーゼと同様，高度に保存されていた．本酵素の熱安定性が P. amyloderamosa 由来イソアミラーゼよりも高
い要因として，P. amyloderamosa 由来イソアミラーゼよりもループの長さが全体的に短いことと，カルシウムイオン結合サイトの欠如が挙げられた．今後さらに構造解析を進めることにより，本酵素の熱安定性機構，反応
機構など更なる知見が得られることが期待される．
kn-abstract=The isoamylase gene from Rhodothermus marinus was cloned into and expressed in Escherichia coli
Top 10. As a result of characterization of purified R. marinus isoamylase. the enzyme had an optimum
pH of 4.0 and optimum temperature of 70℃. Thermal inactivation studies of the purified R. marinus
isoamylase revealed the enzymatic activity to be uninfluenced after one hour incubation at 60℃. These
results suggest that R. marinus isoamylase has high thermostability. The crystallization and crystal
structure analysis of R. marinus isoamylase was performed. The three-dimensional structure at 1.9Å
resolution was determined in complex with the panose. R. marinus isoamylase is composed of three
domains N, A and C, and, has a (β/α)8-barrel in domain A. The secondary structural alignments of the
R. marinus isoamylase and P. amyloderamosa isoamylase was carried out. They have the four active-site
consensus regions characteristic of the α-amylase family. And the essential residue of the α-amylase
family (D359, E395, and D467) was conserved in these enzymes. R. marinus isoamylase has shorter loops
than P. amyloderamosa isoamylase. And R. marinus isoamylase had no Ca2+ binding site. These results
are thought to be factors of thermostability of R. marinus isoamylase.
en-copyright=
kn-copyright=

en-aut-name=TachibanaAkiko
en-aut-sei=Tachibana
en-aut-mei=Akiko
kn-aut-name=立花亜紀子
kn-aut-sei=立花
kn-aut-mei=亜紀子
aut-affil-num=1
ORCID=

en-aut-name=TamuraTakashi
en-aut-sei=Tamura
en-aut-mei=Takashi
kn-aut-name=田村隆
kn-aut-sei=田村
kn-aut-mei=隆
aut-affil-num=2
ORCID=

en-aut-name=ImadaKatsumi
en-aut-sei=Imada
en-aut-mei=Katsumi
kn-aut-name=今田勝巳
kn-aut-sei=今田
kn-aut-mei=勝巳
aut-affil-num=3
ORCID=

en-aut-name=KinoshitaMiki
en-aut-sei=Kinoshita
en-aut-mei=Miki
kn-aut-name=木下実紀
kn-aut-sei=木下
kn-aut-mei=実紀
aut-affil-num=4
ORCID=

en-aut-name=NambaKeiichi
en-aut-sei=Namba
en-aut-mei=Keiichi
kn-aut-name=難波啓一
kn-aut-sei=難波
kn-aut-mei=啓一
aut-affil-num=5
ORCID=

en-aut-name=TsutsumiNoriko
en-aut-sei=Tsutsumi
en-aut-mei=Noriko
kn-aut-name=堤紀子
kn-aut-sei=堤
kn-aut-mei=紀子
aut-affil-num=6
ORCID=

en-aut-name=HashidaMiyoko
en-aut-sei=Hashida
en-aut-mei=Miyoko
kn-aut-name=橋田みよ子
kn-aut-sei=橋田
kn-aut-mei=みよ子
aut-affil-num=7
ORCID=

en-aut-name=SakaguchiHiromichi
en-aut-sei=Sakaguchi
en-aut-mei=Hiromichi
kn-aut-name=坂口博脩
kn-aut-sei=坂口
kn-aut-mei=博脩
aut-affil-num=8
ORCID=

en-aut-name=InagakiKenji
en-aut-sei=Inagaki
en-aut-mei=Kenji
kn-aut-name=稲垣賢二
kn-aut-sei=稲垣
kn-aut-mei=賢二
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学

affil-num=2
en-affil=
kn-affil=岡山大学

affil-num=3
en-affil=
kn-affil=大阪大学

affil-num=4
en-affil=
kn-affil=大阪大学

affil-num=5
en-affil=
kn-affil=大阪大学

affil-num=6
en-affil=
kn-affil=ノボザイムズ ジャパン

affil-num=7
en-affil=
kn-affil=ノボザイムズ ジャパン

affil-num=8
en-affil=
kn-affil=ノボザイムズ ジャパン

affil-num=9
en-affil=
kn-affil=岡山大学
en-keyword=isoamylase
kn-keyword=isoamylase
en-keyword=Rhodothermus marinus
kn-keyword=Rhodothermus marinus
en-keyword=crystal structure
kn-keyword=crystal structure
en-keyword=thermostability
kn-keyword=thermostability
END

start-ver=1.4
cd-journal=joma
no-vol=85
cd-vols=
no-issue=1
article-no=
start-page=79
end-page=88
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19960201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Overcoming the cross-incompatibility through embryo rescue and the transfer of characters within the genus Brassica and between wild relatives and Brassica crops
kn-title=胚救助法による交雑不和合性の克服とBrassica 属内の野生近縁種からBrassica 属作物への形質導入
en-subtitle=
kn-subtitle=
en-abstract=胚救助法による交雑不和合性の克服以外に種属間雑種を作種する方法は、プロトプラストの融合法による異種間雑種作出の方法が考えられる。植物細胞は全形成能を持つのでどんな場合にもプロトプラストから植物体の再分化が感応と考えられたが、異種プロトプラストの融合から雑種個体の再生は染色体の除去や融合細胞の枯死等がおこって、期待どうりには運べなっかた。それでも、最近少しずつ有用植物の異種プロトプラストの融合から植物体の再生が報告された。Brassiceae類においては最近になって新しい種属間雑種の作出が報告された79-85)。このように種属間雑種を作出するのが色々な方法が考えられてきたが、それをうまく利用することにより有用形質をある作物から別の作物へ、あるいは野生植物からの栽培植物へ導入の可能性は大きく広がってきている。さらに、最近では遺伝子組み替えの技術が発達して、植物にもその技術が活用できる可能性が出てきた。しかし、現在では基礎的な研究が主で有用植物に利用できる段階まで達していないが、やがてこの技術も発達して有用植物に他の生物の遺伝子あるいは形質を導入できる事が出来るようになるだろう。
kn-abstract=Cross-incompatibility of interspecific and intergeneric hydrids, and between diploid and polyploid is described in this paper. The causes of the failure in seed development between diploid and autotetraploid Brassica campestris lay in the genome imbalance of the hybrid endosperm. Overcoming this cross-incompatibility through embryo rescue, embryo culture, ovule culture and ovary culture is described. Especially the culture conditions of ovary culture in Brassica campestris, and the production of interspecific and intergeneric hybrids among the genus Brassica is discussd. Many interspecific and intergeneric by hybrids were obtained through embryo rescue. The cytogenetics of the interspecific and intergeneric hybrids, and the crossability of the hybrid progenies were carried out in the ganus Brassica. The cytogenetics of interspecific and intergeneric hybrids showed that a few bivalent formations were always observed at the first meiotic division of the hybrids among Brassiceae. The F1 hybrids almost never had any pollen fertility but many seeds were obtained from open pollination and backcross with their parents. Gene or character transfer within the genus Brassica and between wild relatives and Brassica crops was possible by successive backcrossing.
en-copyright=
kn-copyright=

en-aut-name=InomataNobumichi
en-aut-sei=Inomata
en-aut-mei=Nobumichi
kn-aut-name=猪俣伸道
kn-aut-sei=猪俣
kn-aut-mei=伸道
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=cross-incompatibility
kn-keyword=cross-incompatibility
en-keyword=cytogenetics
kn-keyword=cytogenetics
en-keyword=embryo rescue
kn-keyword=embryo rescue
en-keyword=genus Brassica
kn-keyword=genus Brassica
en-keyword=gene or character transfer
kn-keyword=gene or character transfer
END

start-ver=1.4
cd-journal=joma
no-vol=85
cd-vols=
no-issue=1
article-no=
start-page=89
end-page=98
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19960201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Molecular Genetics of Mammals
kn-title=哺乳類の分子遺伝学
en-subtitle=
kn-subtitle=
en-abstract=近年の分子生物学の発展は、ヒトを含む哺乳類の遺伝学にも大きな変化をもたらしている。すなわち、表現型を主な対象とした従来の遺伝学から、DNAをはじめとする分子を対象とした遺伝学へとその中心は移行しつつある。この傾向に拍車をかけたのがここ数年話題になることの多いヒトゲノムプロジェクトであり、またヒトの遺伝病の原因遺伝子の解析と遺伝子診断、遺伝子治療の進展である。農学や畜産学の分野でも、分子育種という概念が確立しつつあり、本論文ではヒト、家畜および実験動物を対象とした哺乳類の分子遺伝学の最近の動向について、我々の研究結果を含めて概説したい。
kn-abstract=Recent advances in molecular genetics of mammals,including construction of genetic linkage maps, identification of mutations responsible for inheritable diseases, mapping of quantitative trait loci,and findings about sex determination are reviewed in the present paper. Information about genomes of humans,laboratory animals and domestic animals has been rapidly accumulated in the last decade. Establishment of polymorphic linkage markers and construction of chromosomal linkage maps of mammalian species enable us to identify the genes responsible for particular genetic traits. Various mutations have been found in genes responsible for human inheritable diseases. We have constructed genetic linkage maps for rats and identified a mutation responsible for mucopoly saccharidosis in rats. Mapping of loci affecting the quantitative traits of domestic animals, including growth rate, litter size, and milk production, as well as identification of the genes responsible for inheritable diseases of domestic animals, are also being carried out.Recently, the testis-determining gene on the Y chromosome, which determine the sex of mammals by defferentiating the testis in the embryos, have been identified in humans and mice. Functions of the sex-determining gene in sexual differentiation of the mammalian embryos have been intensively analyzed. We have found a sexual difference in the growth rate of mouse preimplantation embryos. An application of the recent findings in the molecular genetics of mammals will contribute to animal science and agricultural science.
en-copyright=
kn-copyright=

en-aut-name=KuniedaTetsuo
en-aut-sei=Kunieda
en-aut-mei=Tetsuo
kn-aut-name=国枝哲夫
kn-aut-sei=国枝
kn-aut-mei=哲夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=chromosome mapping
kn-keyword=chromosome mapping
en-keyword=linkage
kn-keyword=linkage
en-keyword=inheritable disease
kn-keyword=inheritable disease
en-keyword=sex determination
kn-keyword=sex determination
END

start-ver=1.4
cd-journal=joma
no-vol=85
cd-vols=
no-issue=1
article-no=
start-page=69
end-page=77
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1996
dt-pub=19960201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Introduction of Biocatalysis into Chemical Industry
kn-title=生体触媒の化学工業への導入
en-subtitle=
kn-subtitle=
en-abstract=今日のバイオインダストリーは、アルコール発酵をはじめとする醸造発酵工場に端をはっしていると言えよう。発酵工場は、アルコールから有機酸(酢酸、クエン酸など)、有機溶媒(アセトン、ブタノールなど)の生産目的とする化学工場へと発展していった。そしてペニシリンの発見とその発酵生産法の確立が契機となって微生物利用技術は大きく躍進、発展を遂げた。その後、微生物利用工場は勢いついた潮流となて抗生物質、アミノ酸、核酸という3つの大きな山を乗り越えて、工業として確固たる地位築に至る。これらの成果を通じて、微生物利用工場は専らファインケミカルズの生産を対象とする特徴を呈した。従来より、微生物を用いた生産法は化学合成法を凌ぐ場合や競合する事態もしばしばあった。しかし1950年以降の石油化学工場の著しい発展に伴い、大量生産型の化成品を対象とする化学工場においては微生物利用技術は大した意味を持たなくなっていった。しかし、最近になり、微生物反応を化学工場に積極的に導入し、利用しようとする試みが盛んになってきた。これは微生物を触媒として用いる培養、反応系は効率および特異性において化学反応を凌駕し、生成物に純度が高いこと、反応が常温、常圧の温和な条件で行われ環境適応型プロセスであること、生産プラントがコンパクトであり、効率的な省エネルギー型プロセスであることなどに因ることが挙げられる。また今日の地球環境問題を考える時、バイオプロセスは化学工場において、新たな意義を見いだしつつある。さらに遺伝子組み替え技術の著しい発展により、従来の障壁に突破口を開く可能性も具体化しつつある。しかしながら、バイオプロセスの開発は時間がかかるのを常としており、実際の向上化例はまだまだ数少ない。バイオプロセスによってたとえ有用物が生産できても、従来の化学合成プロセスに比べて経済的利点があるのか、新たな設備投資をしてでも採用すべきプロセスなのか、が常に問題となる。本報では、生体触媒を用いた最近の代表的な工場化研究が紹介されている。またここで微生物生産の化学工場における位置付けを概略して述べ、筆者が長年取り組んできた典型的な大量生産型化成品、アクリルアミドの工場生産の開発研究を紹介しつつ、化学工場における生体触媒の問題点と展望を述べてみたい。
kn-abstract=Microbial processes for industrial production of commodity chemicals are rapidly gaining practical significance for preparation of high purity products, in an environmentally acceptable manner, while realizing energy savings. The use of bacterial nitrile hydratase for industrial production of the important chemical, acrylamide, was recently pioneered in Japan. We review here the enzymatic production of acrylamide and recent progress in the production of other commodity chemicals through microbial processes.
en-copyright=
kn-copyright=

en-aut-name=NagasawaToru
en-aut-sei=Nagasawa
en-aut-mei=Toru
kn-aut-name=長澤透
kn-aut-sei=長澤
kn-aut-mei=透
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=biocatalysis
kn-keyword=biocatalysis
en-keyword=commodity chemicals
kn-keyword=commodity chemicals
en-keyword=nitrile hydratase
kn-keyword=nitrile hydratase
en-keyword=acrylamide
kn-keyword=acrylamide
en-keyword=microbial process
kn-keyword=microbial process
END