start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=1 article-no= start-page=31 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680329 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Grouping of elongated plant viruses reported in Japan up to 1967 according to Brandes' system of classification. kn-title=1967年までに報告された本邦産長形植物ウイルスのBrandesの方式による類別 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=2 article-no= start-page=61 end-page=65 dt-received= dt-revised= dt-accepted= dt-pub-year=1964 dt-pub=196403 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The thiobarbituric acid test of 4,5-unsaturated digalacturonic acid. kn-title=4,5-不飽和ヂガラクチュロン酸のチオバルビツール酸反応 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OkamotoK. en-aut-sei=Okamoto en-aut-mei=K. kn-aut-name=岡本賢一 kn-aut-sei=岡本 kn-aut-mei=賢一 aut-affil-num=1 ORCID= en-aut-name=HatanakaC. en-aut-sei=Hatanaka en-aut-mei=C. kn-aut-name=畑中千歳 kn-aut-sei=畑中 kn-aut-mei=千歳 aut-affil-num=2 ORCID= en-aut-name=OzawaJ. en-aut-sei=Ozawa en-aut-mei=J. kn-aut-name=小沢潤二郎 kn-aut-sei=小沢 kn-aut-mei=潤二郎 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199507 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=80年の歴史から21世紀へ向けて : 研究活動と学術的・社会的貢献 : 岡山大学資源生物科学研究所創設80周年記念 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=2 article-no= start-page=71 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=197203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cattleya系の花のえそ症状株から分離されたウイルス en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=1 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ホウレンソウ種子に存在するα-グルコシダーゼの分子多型変化 kn-title=Change in the Multiple Forms of α-Glucosidase from Spinach Seeds en-subtitle= kn-subtitle= en-abstract=4℃保存したホウレンソウ種子から2種のα-グリコシダーゼをCM-セルロースカラムクロマトグラフィーとゲル濾過により精製した。α-グルコシダーゼAとBの分子量はそれぞれSDS-PAGEで78kDA、82kDa、ゲル濾過で62kDa、70kDaであった。α-グルコシダーゼAはマルトオリゴ糖だけではなく可溶性デンプンに対して強い加水分解活性を示した。至適pHは4.5-5.5であり、65℃、20分処理後に約50%の残存活性を示した。一方、α-グルコシダーゼBはマルトオリゴ等に対して強い加水分解活性を示したが、可溶性デンプンに対してほとんど活性を示さなかった。至適pHは5.0であり、65℃、20分処理後に活性を消失した。α-グルコシダーゼAとα-グルコシダーゼBは、4℃保存を行わない種子に見出されたα-グルコシダーゼT、Uとα-グルコシダーゼV、Wにそれぞれ酵素化学的性質、免疫化学的性質が類似していた。これらの結果、ホウレンソウ種子に存在するα-グルコシダーゼの分子多型は2種類に収束されることが示唆された。 kn-abstract=Two molecular forms of α-glucosidase were isolated from spinach seeds after storage at 4℃ by CM-cellulose column chromatography and gel filtration. The molecular masses of α-glucosidase A and B were 78 kDa and 82 kDa by SDS-PAGE, and 62 kDa and 70 kDa by gel filtration, respectively. α-Glucosidase A had high activity not only toward maltooligosaccharides but also toward α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 65℃ for 20 min. On the other hand, α-glucosidase B had high activity toward maltooligosaccharides but faint activity toward α-glucans. The optimum pH was 5.0 and no activity was found after incubation at 65℃ for 20 min. The enzymatic and immunological properties of α-glucosidase A and B were similar to those of α-glucosidase. Tor U, and α-glucosidase V or W, isolated from spinach seeds without 4℃ storage, respectively. These findings suggest that the α-glucosidase in spinach seeds is modified to be two molecular forms. en-copyright= kn-copyright= en-aut-name=FuruiSatoshi en-aut-sei=Furui en-aut-mei=Satoshi kn-aut-name=古井聡 kn-aut-sei=古井 kn-aut-mei=聡 aut-affil-num=1 ORCID= en-aut-name=SugimotoManabu en-aut-sei=Sugimoto en-aut-mei=Manabu kn-aut-name=杉本学 kn-aut-sei=杉本 kn-aut-mei=学 aut-affil-num=2 ORCID= en-aut-name=SuzukiYukio en-aut-sei=Suzuki en-aut-mei=Yukio kn-aut-name=鈴木幸雄 kn-aut-sei=鈴木 kn-aut-mei=幸雄 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Spinach kn-keyword=Spinach en-keyword=α-Glucosidase kn-keyword=α-Glucosidase en-keyword=Multiple molecular forms kn-keyword=Multiple molecular forms END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=135 end-page=144 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 kn-title=Characteristics of Pectic Polysaccharides from Rice Shoots en-subtitle= kn-subtitle= en-abstract=イネ幼植物から調整した細胞壁から、50mMの1,2−シクロヘキサンジアミン四酢酸と炭酸ナトリウムにて、ペクチン質を化学的に抽出した。抽出される全ペクチン質に含まれる糖含量は、乾燥細胞壁重の約1%に相当した。両者の薬剤で抽出されたペクチン質をDEAE−Trisacryl Mカラムトグラフィーによって、5種の多糖画分に分画し、主要3画分の構成糖組成と分子量を検討した。1,2−シクロヘキサンジアミン四酢酸で抽出され上記カラムに未吸着の中性多糖画分は、アラビノース、ガラクトースから成り、分子量56kDaであった。また、炭酸ナトリウムで抽出されカラムに未吸着の中性多糖画分は、アラビノース、キシロース、ガラクトースから成り、分子量30kDaであった。一方、酸性多糖画分は、中性糖側鎖を持った、ラムノガラクツロナンとホモガラクツロナンを含んでいた。Erwinia carotovora Er.からのエンド−ペクチン酸リアーゼとKluyveromyces fragilisからのエンド−ポリガラクツロナーゼの精製標品を、イネ細胞壁に作用させたところ、0.4〜0.6%に相当するペクチン質断片が可溶化された。一方、上述したカラムトグラフィーで得られた82kDaのラムノガラクツロナンにエンド−ポリガラクツロナーゼを作用させると、多量のガラクツロン酸が遊離され、また、未分画分は90%以上のガラクトースとアラビノースから成っていた。この結果から、この酸性多糖画分の”hairly”領域は、エンド−ポリガラクツロナーゼの作用できないガラクトースとアラビノースのみから成る側鎖であった。 kn-abstract=Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4〜0.6% of the starch-free cell walls. en-copyright= kn-copyright= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=1 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=2 ORCID= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Cell wall kn-keyword=Cell wall en-keyword=Oryza sativa kn-keyword=Oryza sativa en-keyword=Pectic polymer kn-keyword=Pectic polymer END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=201 end-page=213 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ixia から分離された bean yellow mosaic virus kn-title=Characterization of Bean Yellow Mosaic Virus from Ixia hybrida en-subtitle= kn-subtitle= en-abstract=1992年に岡山県倉敷市玉島で、葉に斑入りを生じた球根類花卉植物Ixia hybridaからpotyvirus(Ixia-B)が分離され、その諸性質から bean yellow masaic virus(BYMV)と同定された。本ウイルスを11科47種の植物に接種したとこと、フリージャ、Nicotiana clevelandii、Chenopodium amaranticolar、ソラマメ、クリムソンクローバー、インゲンマメ、ホウレンソウに全身感染し、またC.quinoa、フダンソウ、ツルナ、センニチコウなどに局部感染したが、エンドウ、ササゲ、ダイズなどには感染しなかった。本ウイルスをはモモアカアブラムシにより非永続的伝搬され、C.quinoa の病葉粗汁液中での安定性は耐熱性が55℃〜60℃(10分)、耐希釈性10-3〜10-4、耐保存性2〜4日であった。DN法試料の電顕観察で多くのpotyvirusよりやや長い約820nmのひも状粒子と管状封入体の破片が見られた。感染葉の超薄切片では風車状、層板状の封入体、dence body、細胞質に散在するウイルス粒子が観察された。本ウイルスはfreesia mosaic virusおよびclover yellow vein virusの抗血清と反応せず、また本ウイルス抗血清を用いた寒天ゲル内二重拡散法ではBYMV分離株(Cro-4, BYMV-G)と反応したが、本ウイルスとBYMVのCro-4およびBYMV-G間にspurが形成され、異種抗原の存在が認められた。外被タンパク質の分子量は約34Kで、ssRNAのサイズは約9Kbであった。Papain,chymotrypsin,pepsinを用いた外被タンパク質のぺプタイドマッピングでは、本ウイルス、Cro-4、BYMV-G、Cal-35の部分分解パターンがそれぞれ異なり、外被タンパク質がアミノ酸配列レベルで異なっていることが示唆された。 kn-abstract=A strain (Ixia-B) of bean yellow mosaic virus (BYMV) isolated from Ixia hybrida was characterized and compared with other isolates of BYMV and clover yellow vein virus (CYVV). Ixia-B was transmitted by aphids,Myzus presicae in a non-presistent manner and by sap-inoculation to 11 of 46 species in 5 of 10 families tested, and had a similar host range to that of some BYMV isolates, althrough some defferences were detected. Sap from diseased C. quinoa was infective after 10 min heating at 55℃ but not 60℃, after a dilution to 10-3 but not 10-4, and after 2 days but not 4 days at 20℃.The Virus particles were filamentous rods of about 13×820 nm. Ixia-B contaied a single protein species with a molecular weight of 34,000 and a single viral RNA with approximately 9,000 bases. In ultrahtin sections of leaf tissues from infected plants, the virus particles, cylindrical cytoplasmic inclusions and dense bodies were obsserved in the cytoplasm. The antiserum to Ixia-B produced by immunizing a rabbit had a titer of 1/512. A close serological relationship was revealed between Ixia-B and two strains of BYMV from crocus and gladiolus, but no relationship to clover yellow vein virus was found in agar gel diffusion tests. However,Ixia-B could be distinguished from two strains of BYMV by the formation of spurs among them in agar gel and by the differences in the patterns of peptide mapping of coat proteins. From these findings, Ixia-B was identified as a strain of BYMV. en-copyright= kn-copyright= en-aut-name=TsujiToshiya en-aut-sei=Tsuji en-aut-mei=Toshiya kn-aut-name=辻俊也 kn-aut-sei=辻 kn-aut-mei=俊也 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=3 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Ixia hybrida kn-keyword=Ixia hybrida en-keyword=Bean yellow mosaic virus kn-keyword=Bean yellow mosaic virus en-keyword=Potyvirus kn-keyword=Potyvirus END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=58 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The cladosporium leaf mold of chestnut caused by Cladosporium epiphillum (Pers.) Mart. kn-title=Cladosporium epiphillum (Pers.) Mart.によるクリの葉カビ病(新称)について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YoshitomiK. en-aut-sei=Yoshitomi en-aut-mei=K. kn-aut-name=吉富C志 kn-aut-sei=吉富 kn-aut-mei=C志 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=091 end-page=103 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 kn-title=Comparison of Absorption Rates between Ammonium and Nitrate Nitrogen in Plants en-subtitle= kn-subtitle= en-abstract=硝酸アンモニウム2.5mMの単塩溶液(以下、硝安単塩溶液)及び2.5mMの硝酸アンモニウムを窒素源とする完全培地(以下、硝安完全培地)を吸収溶液とし、アンモニア態窒素及び硝酸態窒素について24時間の吸収実験を行った。実験材料はイネ、オオムギ、レタス、キュウリ、ニンジン、ハクサイ、ホウレンソウ、ダイコンであった。硝安単塩溶液及び硝安完全培地における両形態窒素の吸収特性から、試験植物は次のように分類できた。(1)イネ、オオムギ、レタス:何れの吸収溶液からでも、硝酸態窒素に比較して常にアンモニア態窒素を速やかに吸収する植物群、(2)キュウリ、ニンジン:硝安単塩溶液ではアンモニア態窒素を速やかに吸収するが、硝安完全培地ではこの傾向が逆転し、硝酸態窒素の吸収がやや速やかになる植物群、(3)ハクサイ、ホウレンソウ、ダイコン:硝安単塩溶液では両形態の窒素の吸収速度はほぼ等しいが、硝安完全培地では明らかに硝酸態窒素の吸収が速やかになる植物群。上記の分類は、それぞれの培地におけるアンモニア態窒素の吸収と拮抗する他のイオンの吸収特性、換言すれば、NO3-/NH4+吸収比、あるいは、Ca2+とMg2+の合計吸収速度、または、植物根の硝酸態窒素に対する相対的な根の親和性、つまり、NO3-/H2PO4-吸収比によって説明できた。すなわち、第1群は1価陽イオン、特に、アンモニウムイオンに対する根の親和性は大きいが硝酸態窒素に対する根の親和性は小さく、また、耐酸性、耐アンモニア性の大きい植物と考えられ、24時間の吸収時間中の培地pHの低下は著しかった。これに対して、第3群は硝安完全培地中では硝酸態窒素、2価陽イオンに対する根の親和性の大きい植物であり、また第2群は、アンモニア態及び硝酸態窒素、ならびに、2価陽イオンに対する根の親和性などが第1群と第3群の中間の植物であった。 kn-abstract=Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. en-copyright= kn-copyright= en-aut-name=MoritsuguMasumi en-aut-sei=Moritsugu en-aut-mei=Masumi kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=1 ORCID= en-aut-name=KawasakiToshio en-aut-sei=Kawasaki en-aut-mei=Toshio kn-aut-name=河ア利夫 kn-aut-sei=河ア kn-aut-mei=利夫 aut-affil-num=2 ORCID= en-aut-name=SuzukiTakao en-aut-sei=Suzuki en-aut-mei=Takao kn-aut-name=鈴木孝夫 kn-aut-sei=鈴木 kn-aut-mei=孝夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Alkaline earth kn-keyword=Alkaline earth en-keyword=Ammonium kn-keyword=Ammonium en-keyword=Ion balance kn-keyword=Ion balance en-keyword=Nitrate kn-keyword=Nitrate en-keyword=Phosphate kn-keyword=Phosphate END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=31 end-page=38 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=日本におけるCymbidium属植物から分離されて生物学的性質の異なるOdontoglossum Ringspot Virus分離株のペプチドマッピングによる比較 kn-title=Comparison of Biologically Different Isolates of Odontoglossum Ringspot Virus from Cymbidium in Japan by Peptide Mapping en-subtitle= kn-subtitle= en-abstract=日本においてCymbidium属植物から分離されたodontoglossum ringspot virus (ORSV)5分離株間の比較を行った。接種試験の結果、Cy-1分離株(井上 1966)はCymbidiumでの病徴が他の4分離株と異なっていた。外被タンバク質のペプチドマッピングでは、ペプシンを用いた場合Cy-1分離株において特異的な部分分解バンドが認められ、そのバンドパターンが他の分離株と大きく異なった。また、V8プロテアーゼによる部分分解パターンも分離株間でわずかに異なったが、キモトリプシンとパパインでは差は認められなかった。ORSV感染葉から2本鎖RNAを抽出しPAGEを行ったところ、すべての分離株で分子量が4.3、1.4、0.6×106の3種バンドが検出されたが、これらの2本鎖RNAの分子量は分離株間で差がなかった。 kn-abstract=Symptoms on Cymbidium, double-stranded (ds) RNA pattern and peptide mapping of coat protein (CP) of five isolates of odontoglossum ringspot virus from Cymbidium in Japan were compared. One of the isolates, Cy-1, that produced unique symptoms on Cymbidium, showed a distinct peptide mapping pattern from those of the other four isolates by partial digestion of CP with pepsin. All the isolates produced three major dsRNA species of Mr=4.3,1.4 and 0.6×106 in the infected plants. en-copyright= kn-copyright= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Odontoglossum ringspot virus kn-keyword=Odontoglossum ringspot virus en-keyword=Cymbidium kn-keyword=Cymbidium en-keyword=Peptide mapping kn-keyword=Peptide mapping en-keyword=Double-stranded RNA kn-keyword=Double-stranded RNA END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=2 article-no= start-page=89 end-page=97 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680624 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Some experiments on the transmission of Cymbidium mosaic virus and Odontoglossum ringspot virus kn-title=Cymbidium mosaic virusおよびOdontoglossum ringspot virusの伝搬に関する2,3の実験 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=2 article-no= start-page=53 end-page=67 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Host range and properties of a strain of odontoglossum ringspot virus in Japan kn-title=Cymbidiumから分離されたodontoglossum ringspot virusの1系統の性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=49 end-page=60 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Dendrobiumから分離されたCucumber mosaic virus en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=109 end-page=118 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=インドネシアのランに発生するウイルスの検索と同定 kn-title=Detection and Identification of Viruses of Orchids in Indonesia en-subtitle= kn-subtitle= en-abstract=1989年8月と1990年7月の2回にわたって、インドネシア国のランに発生するウイルス病の調査研究を行った。5地域12ヵ所のラン園または温室で採集したランのうち、特徴のある病斑を示した14属43個体について、病徴、血清反応、電顕によるウイルス粒子の形態を調べ、また一部の試料については病葉超薄切片の電顕観察を行ってウイルスの診断同定を行った。その結果、Cymbidium mosaic virus(CyMV), odontoglossum ringspot virus (ORSV)および未同定 potyvirusの3種のウイルスの発生が確認された。CyMV は Aranthera,Calanthe,Cattleya,Cymbidium,Grommatophyllum,Onicidium,Phalaenopsis,Vanda の8属から、ORSVはBulbophyllum、Calanthe,Cattleya,Oncidium,Phalaenopsis の5属から、また未同定のpotyvirusはArantheraから検出された。ORSVおよび未同定のpotyvirusはCyMVと混合感染しているものが多かった。以上の調査研究から、インドネシアではCyMVが各地に広く発生分布し、ORSVとともに被害の大きいウイルス病の病原であることが認められた。 kn-abstract=Three viruses, Cymbidium mosaic virsu(CyMV), odontoglossum ringspot virus (ORSV) and an unidentified potyvirus were found in the orchids in Indonesia. CyMV was detected from orchids in 8 genera, namely Aranthera, Calanthe, Cattleya, Cymbidium, Gromatophyllum, Phalaenopsis,Oncidium and Vanda. The virus was widespread in many orchids in Indonesia and was common in Aranthera and Calanthe, thus being an economically important virus in Indonesia.ORSV was also detected in orchids of 5 genera, namely Bulvophyllum,Calanthe, Cattleya,Oncidium and Phalaenopsis. The unidentified potyvirus was found in Aranthera. en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=GaraI Wayan en-aut-sei=Gara en-aut-mei=I Wayan kn-aut-name=ガライ ワヤン kn-aut-sei=ガラ kn-aut-mei=イ ワヤン aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Cymbidium mosaic virus kn-keyword=Cymbidium mosaic virus en-keyword=Odontoglossum ringspot virus kn-keyword=Odontoglossum ringspot virus en-keyword=Unidentified potyvirus kn-keyword=Unidentified potyvirus en-keyword=Identification kn-keyword=Identification en-keyword=Orchids in Indonesia kn-keyword=Orchids in Indonesia END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue=1 article-no= start-page=53 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=1999 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ライムギ小型染色体を保持する普通系コムギからのライムギ型cDNAのディファレンシャルスクリーニング kn-title=Differential Screening of Rye-type cDNAs from a Common Wheat Carrying the Rye Midget Chromosomes en-subtitle= kn-subtitle= en-abstract=ライムギの細胞質を有する普通系コムギ(cereale)-Chinese Spring (CS)には、「ミジェット」と呼ばれる非常に小型の染色体が存在する。この染色体が失われると、その種子は胚乳が退化して発芽出来ない。かりに発芽しても、その植物は虚弱で不稔となる。このことは、この染色体にライムギ細胞質の機能維持に不可欠な遺伝子が座乗していることを示唆している。本研究では、ディファレンシャルスクリーニング法により、このミジェット染色体に座乗している遺伝子の同定を試みた。(cereale)-CSの若苗からcDNAライブラリーを作成し、約20,000のプラークをスクリーニングした。その結果、ライムギのcDNAには強くハイブリダイズするが、CSのcDNAには弱くハイブリダイズするクローンが27得られた。これらについてその特異性をサザンハイブリダイゼイション等で解析したところ、19個のクローン( CrClAなど) はサイズ、制限酵素部位に違いは認められるもののお互いにハイブリダイズすることから、同じクラスTに分類された。これらは、正常CSと(cereale)-CSとの間でRELP(制限酵素断片長多型)を示し、その型はライムギと同様であった。このcDNAの塩基配列は、コムギ葉緑体DNA遺伝子rbcLに類似しており、ライムギの葉緑体DNAに由来していると考えられた。他のクローンは、コムギの核遺伝子cabとrbcにその塩基配列が類似していたが、はっきりとした多型は検出されなかった。ここで用いた方法では、光合成に関連した発現量の多いcDNAがスクリーニングされやすい傾向があり、効率的なスクリーニングには均一化したcDNAを用いる必要があると思われる。 kn-abstract=Occurrence of the midget chromosome in a common wheat with rye cytoplasm [(cereale)-Chinese Spring (CS)] indicates that the chromosome carries the essential gene(s) for maintaining the function of rye cytoplasm. To elucidate the interaction between the midget chromosome and rye cytoplasm, in this study, an attempt was made to isolate rye-type cDNAs from a cDNA library of (cereale)-CS by differential screening. Two replica filters from each plate were hybridized with digoxigenin (DIG)-labeled wheat CS cDNAs and with DIG-labeled rye cDNAs,respectively. Out of ca. 20,000 plaques, 27 were hybridized more strongly with rye cDNAs than with CS cDNAs. These clones were classified into six classes (T-Y) by blot hybridization. The majority of the clones (21 out of 27) was belonged to the same class (1), showing rye-type RFLP (restriction fragment length polymorphism). The DNA sequence of clone CrClA in class T, was very similar to that of wheat ribulose 1,5-bisphosphate carboxylase,large subnit gene, rbcL(94.5% homology). However, the 3' end of CrClA was shorter than that of wheat rbcL, and terminated at TAA instead of TAG, like the rbcL of Aegilops crassa. In the clone CrC5.4, the first half of the sequence was similar to that of one rice EST clone, the functions of which are not known, and the latter was similar to the reverse sequence of maize 4.5S-23S ribosomal RNA. This suggests that CrC5.4 had been derived from two defferent cDNAs of (cereale)-CS. Three other clones had homology to the chlorophyll a/b binding protein genes (cab) of wheat, maize and tomato, and one to wheat rbcS (ribulose1,5-bisphosphate carboxylase small subnit gene). However, no clear polymorphisms were detected between wheat and rye by using those clones as probes. en-copyright= kn-copyright= en-aut-name=MurataMinoru en-aut-sei=Murata en-aut-mei=Minoru kn-aut-name=村田稔 kn-aut-sei=村田 kn-aut-mei=稔 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=Cytoplasm substitution line kn-keyword=Cytoplasm substitution line en-keyword=Differential screening kn-keyword=Differential screening en-keyword=Midget chromosome kn-keyword=Midget chromosome en-keyword=Rye kn-keyword=Rye en-keyword=Wheat kn-keyword=Wheat END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=2 article-no= start-page=53 end-page=65 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=197503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characteristics and inheritance of EMS-induced mutants in barley I. A semidominant chlorophyll mutant, several glossy sheath mutants and a smooth awned mutant kn-title=EMS処理で得た大麦変異体の特性と遺伝 I.半優性葉緑素変異体,glossy sheath変異体および滑芒変異体 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonishiT. en-aut-sei=Konishi en-aut-mei=T. kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=4 article-no= start-page=209 end-page=220 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on induced mutation in barley by EMS. I. Comparative effects of several EMS treatments on chlorophyll mutation. kn-title=EMS処理による大麦の人為突然変異に関する研究 第1報 葉緑素変異誘発効果について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonishiT. en-aut-sei=Konishi en-aut-mei=T. kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=3 article-no= start-page=141 end-page=152 dt-received= dt-revised= dt-accepted= dt-pub-year=1970 dt-pub=197001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=EMS処理による大麦の人為突然変異に関する研究 第3報 数種の矮性変異体の遺伝分析 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=2 article-no= start-page=45 end-page=59 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=197203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=EMS処理による大麦の人為突然変異に関する研究 第4報 数種の矮性遺伝子の作用およびそれら矮性遺伝子の渦性遺伝子との働きあい en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page=17 end-page=29 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=197303 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on EMS-induced mutations in barley V. Effects of several dwarf genes on lodging resistance and yielding ability kn-title=EMS処理による大麦の人為突然変異に関する研究 第5報 数種の矮性遺伝子の耐倒伏性および収量性に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonishiT. en-aut-sei=Konishi en-aut-mei=T. kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=3 article-no= start-page=155 end-page=166 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on EMS-induced mutations in barley VI. Interactions among several dwarf mutant genes kn-title=EMS処理による大麦の人為突然変異に関する研究 第6報 矮性変異遺伝子相互間の働きあい en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonishiT. en-aut-sei=Konishi en-aut-mei=T. kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=3 article-no= start-page=99 end-page=109 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19681015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on EMS induced mutations in barley II. Induction of dwarf mutations and characteristics of their mutants kn-title=EMS処理による大麦の人為突然変異に関する研究 第2報 矮性変異の誘発とそれらの変異体の特性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonishiT. en-aut-sei=Konishi en-aut-mei=T. kn-aut-name=小西猛朗 kn-aut-sei=小西 kn-aut-mei=猛朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=1 article-no= start-page=1 end-page=8 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=硫化カドミウムの高濃度添加時における小麦の生育とカドミウム集積濃度に対する炭酸カルシウムの影響 kn-title=Effect of Calcium Carbonate on the Cadmium Content of Wheat after Addition of Large Ammount of CdS to Soil en-subtitle= kn-subtitle= en-abstract=植物に対する有害金属の生育限界濃度域での実験は極めて少ない。ここでは、小麦の硫化カドミウム(CdS)に対する生育限界濃度と小麦穀粒中カドミウム含有量に及ぼす炭酸カルシウム添加栽培の影響を調べた。その結果、硫化カドミウムに対する小麦の生育限界濃度は1,500ppmであり、その時の小麦穀粒中のカドミウム含有量は123.2μg/g(乾燥物中)であることが判明した。すなわち溶解度の低いカドミウムの化学形においても、穀粒中への集積が生じる結果が表示された。また、炭酸カルシウムの同時添加により、無添加に対して1,500ppm区では穀粒中のカドミウム含有量は57%減少し、小麦の生育限界濃度5,000ppmに拡大された。茎葉長、茎葉重、穀粒重等の各生育項目において炭酸カルシウムの添加効果が見られ、特にカドミウム濃度500-5,000ppmの間では炭酸カルシウム添加によるカドミウム毒性の抑制現象が有意に認められた。このことは高濃度カドミウム汚地域における炭酸カルシウムによる植物保護の一策としての有効性を示唆したと考えられる。 kn-abstract=In Japan, wheat is usually cultivated from December to June as a second crop in the same field as rice. In Cd-polluted areas, the fields are affected by both wastewater and the emissions from the chimneys of zinc refineries or plating factories. The application of inhibition agents, such as slaked lime, calcium silicate or fused magnesium are applied to inhibit the absorption of these metals by plants. Many reports have been made on the decrease of metal absorption through the application of some form of calcium, phosphate or silicate. Also, it has been reported that wheat is more sensitive to CdS than to CdO. However, almost all experiments were performed at less than 500 ppm Cd in soil, and these experiments have not been made at a concentration close to the level critical for wheat plants. There have been few reports on the effects of calcium cabonate on metal tolerance or metal uptake at the a level critical wheat. The typical example of using calcium carbonate was investigated by Maeda, and the application of phosphate by Koshino, and by Muramoto et al. The effects of calcium carbonate on the metal content of wheat to the critical levels were examined. en-copyright= kn-copyright= en-aut-name=MuramotoShigeki en-aut-sei=Muramoto en-aut-mei=Shigeki kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= en-aut-name=NishizakiHisao en-aut-sei=Nishizaki en-aut-mei=Hisao kn-aut-name=西崎日佐夫 kn-aut-sei=西崎 kn-aut-mei=日佐夫 aut-affil-num=2 ORCID= en-aut-name=AoyamaIsao en-aut-sei=Aoyama en-aut-mei=Isao kn-aut-name=青山勲 kn-aut-sei=青山 kn-aut-mei=勲 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=181 end-page=190 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=タバコ懸濁培養細胞におけるアルミニウムの二価鉄、銅およびカドミウム毒性に対する影響 kn-title=Effects of Aluminum on the Toxicity of Iron(II), Copper and Cadmium in Suspension-cultured Tobacco Cells en-subtitle= kn-subtitle= en-abstract=二価鉄[Fe(U)]、銅(Cu)およびカドミウム(Cd)の細胞毒性に対するアルミニウム(Al)の効果について検討した。120μM AlCl3の存在もしくは非存在下において、対数増殖期の細胞をFeSO4,CuSO4,CdCl2で各々18時間、pH4.0で処理した。処理後の生存率は、処理後の細胞を増殖させたのちに、未処理細胞(コントロール)の増殖に対する金属で処理した細胞の相対増殖率で求めた。Al,Fe(U),Cdの単独処理による増殖阻害は、各々300μM、200μM、500μMの濃度まで観察されなかったが、Cuでは15μMで大きく阻害された。このように、タバコ細胞は相対的にAl、Fe(U)、Cdには感受性が低く、Cuには感受性が高かった。細胞をFe(U)およびAlの両方で処理すると、増殖は著しく阻害され、AlおよびFeの細胞内含量も相乗的に増加した。Cuで処理した場合、Alを加えることにより逆に増殖率が増加した。しかし、細胞内Cu含量はAlの影響を受けなかった。Cd処理の場合、Alを加えてもCdによる増殖阻害の程度は変わらなかった。このように、AlはFe(U)、Cu、Cd毒性に対して各々異なる相互作用を示し、Fe(U)に対し、相乗効果を、Cuに対しては拮抗的な阻害を示したが、Cdに対してはみかけ上効果がなかった。 kn-abstract=The effects of aluminum (Al) on the cytotoxicity of ferrous iron (Fe(U)), copper(Cu) and cadmium(Cd) were studied. Log-phase cells were treated with either FeSO4,CuSO4, or CdCl2 in the presence or absence of AlCl3(120μM) for 18h at pH 4.0. After the treatment, the viability was determined as relative growth of the metal-treated cells to the untreated control cells during the post-treated culture. A single treated with either Al, Fe(U) or Cd did not inhibit the growth at the metal concerntrations up to 300 μM, 200 μM and 500 μM, respectively, whereas the growth was markedly inhibited at 15 μM Cu. Thus,the cells were relatively insensitive to Al, Fe(U) and Cd and sensitive to Cu. When cells were treated with both Fe(U)(120 μM)and Al(120μM), the growth was significantly inhibited and the cellular contents of both Al and Fe increased synergistically. After the treatment with Cu(0 to 10 μM) with or without Al, the cells grew more vigorously when they were treated in the presence of Al, althrouh the Cu content of the cells were not alterd by Al. The presence of Al during the treatmemt with Cd(0 to 2 μM) had no effect on the degree of growth inhibition by Cd. Thus, Al interacts with the toxicity of Fe(U), Cu and Cd in different manners; synergistic with Fe(U), antagonistic with Cu and apparently no effeco on Cd. en-copyright= kn-copyright= en-aut-name=YamamotoYoko en-aut-sei=Yamamoto en-aut-mei=Yoko kn-aut-name=山本洋子 kn-aut-sei=山本 kn-aut-mei=洋子 aut-affil-num=1 ORCID= en-aut-name=ChangYi-Chieh en-aut-sei=Chang en-aut-mei=Yi-Chieh kn-aut-name=張藝潔 kn-aut-sei=張 kn-aut-mei=藝潔 aut-affil-num=2 ORCID= en-aut-name=OnoKanji en-aut-sei=Ono en-aut-mei=Kanji kn-aut-name=小野寛治 kn-aut-sei=小野 kn-aut-mei=寛治 aut-affil-num=3 ORCID= en-aut-name=MatsumotoHideaki en-aut-sei=Matsumoto en-aut-mei=Hideaki kn-aut-name=松本英明 kn-aut-sei=松本 kn-aut-mei=英明 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Aluminum kn-keyword=Aluminum en-keyword=Antagonistic kn-keyword=Antagonistic en-keyword=Nicotiana tabacum kn-keyword=Nicotiana tabacum en-keyword=Suspension culture kn-keyword=Suspension culture en-keyword=Synergistic kn-keyword=Synergistic END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=1-2 article-no= start-page=67 end-page=73 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=196512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sexual compatibility between form species of Erysiphe graminis DC. and viability of the ascospores derived from the inter-form-specific hybridization. kn-title=Erysiphe graminis DC.のform species間の和合性およびform species間交雑により形成された子のう胞子の寄生力 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HiuraU. en-aut-sei=Hiura en-aut-mei=U. kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=4 article-no= start-page=239 end-page=247 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Genetics of host-parasite interaction in formae speciales of Erysiphe graminis kn-title=Erysiphe graminisのformae specialesとそれらの宿主との相互関係の遺伝 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HiuraU. en-aut-sei=Hiura en-aut-mei=U. kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=1 ORCID= en-aut-name=HetaH. en-aut-sei=Heta en-aut-mei=H. kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=39 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ラン科植物に発生するシンビジウムモザイクウイルスの血清学的検出 kn-title=Evaluation of Dot-Immunobinding Assay and Rapid Immunofilter Paper Assay for Detection of Cymbidium Mosaic Virus in Orchids en-subtitle= kn-subtitle= en-abstract=Dot-immunobinding assay (DIA)法とrapid immunofilter paper assay (RIPA)法の2種の血清学的診療法をcymbidium mosaic virus (CyMV)の検出に適用したところ、両方法とも少なくとも純化ウイルスで100ng/ml、罹病葉汁液では10-4希釈まで検出可能であった。DIA法では検出に5〜6時間要したが、本法は多量の資料の診断に適していると思われた。一方、RIPA法では本ウイルスをDIA法と同程度の検出感度で数分以内で正確に検出できたことから、本法は今後のCyMVの簡易・迅速診断法として多種属のラン科植物に広く活用できると考えられた。さらにRIPA法ではCyMVとORSVに重複感染した植物から両ウイルスを同時に検出できた。 kn-abstract=Dot-immunobinding assay (DIA) on nitrocellulose membranes and rapid immunofilter paper assay (RIPA) were examind for their usefulness in the detection of cymbidium mosaic virus (CyMV) in orchids. The minimum detection levels of CyMV by these methods were 100 ng/ml in purified preparations and at 10-4 dilution of extracts from infected leaves of orchids could be detected by these methods. Although DIA took 5 to 6 hours for the detection of the virus, it was reliable method for diagnosis of a large-number of samples. On the other hand, RIPA, which enabled detection of CyMV within a few minutes with sensitivity similar to that of DIA, will be suitable as a rapid and handy tool for virus disease diagnosis in orchids. Moreover, by RIPA, we could detect CyMV and odontoglossum ringspot virus (ORSV) simultaneously form doubly infected plant. en-copyright= kn-copyright= en-aut-name=I WayanGara en-aut-sei=I Wayan en-aut-mei=Gara kn-aut-name=イ ワヤンガラ kn-aut-sei=イ ワヤン kn-aut-mei=ガラ aut-affil-num=1 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=2 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Serological detection kn-keyword=Serological detection en-keyword=Cymbidium mosaic virus kn-keyword=Cymbidium mosaic virus en-keyword=Orchid kn-keyword=Orchid END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=91 end-page=103 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ニンジン懸濁培養細胞の培養液に分泌される細胞外多糖成分 kn-title=Extracellular Polysaccharides from the Culture Medium of Cell Suspension Cultures of Carrot en-subtitle= kn-subtitle= en-abstract=植物培養細胞は懸濁(液体)培養を行うことによって、細胞壁構成多糖成分と構造的に類似している多糖成分を、その培地中に分泌することが知られており、これまで数種の植物培養細胞について報告されている。そこで、これまで研究例のないニンジン培養細胞を使用して、その細胞外多糖成分について検討した。ニンジン懸濁培養細胞の対数増殖後期の培養濾液からエタノール分画によって多糖成分を集め、DEAE-Sepharose CL-6およびDEAE-Trisacry Mカラムクロマトグラフィーによって、数種の多糖画分に分画し、それら画分の構成糖組成を検討した。また、上記カラムに吸着しない中性多糖分画は、分子量52kDaと40kDaの2種の画分から成り、Trichoderma virideから分子量52kDaの画分はキシログルカンであると示唆された。一方、上記カラムに吸着する酸性多糖画分は、DEAE-Trisacryl M カラムクロマトグラフィーによって数種の画分に分画された。その中で、分子量80kDaを示す主要画分は、Erwinia carotovora Er.から精製したエンド‐ペクチン酸リアーゼ、Kluyveromyces fragilisから精製したエンド‐ポリガラクチュロナーゼ、ニンジン培養細胞から精製したα-L-アラビノフラノシダーゼやβ-ガラクトシダーゼなどによって分解されなかった。しかし、50mMのトリフルオロ酢酸による分解にて、4%に相当するガラクトースを含む生成物が遊離された。これらの結果から、この酸性多糖画分は、複雑な中性糖側鎖の結合したラムノガラクチュロナン領域(”Hairy”region)であることが示唆された。 kn-abstract=The extracellular polysaccharides have been fractionated from the culture medium of carrot(Daucus carota)cell culrures by precipitation with ethanol and by chromatography on DEAE-Sepharose CL-6B DEAE-Trisacryl M ion-exchange and Bio-Gel A-1.5m gel-permeation.The sugar composition and molecular mass of purified neutral and acidic polymers were determined. The neutral and acidic polymers were treated with purified endo-Β-glucanase from Trichoderma viride and pectic depolymerases,such as endo-pectate lyase from Erwinia carotovora Er. and endo-polygalacturonase from Kluyveromyces fragilis, respectively. The "hairly"(ramified)regions of acidic polymer were sequentially treated with purified α-L-arabinofuranosidase and β-galactosidase from carrot cell cultures, and were further hydrolyzed with 50mM trifluoroacetic for 1 hr at 100℃. From these results, the extracellular polysaccharides secreted from carrot cell cultures are charactarized. en-copyright= kn-copyright= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=1 ORCID= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=2 ORCID= en-aut-name=KatohKenji en-aut-sei=Katoh en-aut-mei=Kenji kn-aut-name=加藤研治 kn-aut-sei=加藤 kn-aut-mei=研治 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Carrot kn-keyword=Carrot en-keyword=Cell suspension culture kn-keyword=Cell suspension culture en-keyword=Cell wall kn-keyword=Cell wall en-keyword=Extracellular polysaccharides kn-keyword=Extracellular polysaccharides en-keyword=Pectic polymer kn-keyword=Pectic polymer END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=164 end-page=174 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=バンダから分離されたCymubidium Mosaic Virusの諸性質 kn-title=Further Characterization of Cymbidium Mosaic Virus from Vanda Orchid en-subtitle= kn-subtitle= en-abstract=日本において葉にえそ斑やえそ輪紋を示すバンダから分離されたウイルスは、宿主範囲、病葉汁液中での安定性、粒子長、物理化学的性質、外被タンパク質ならびにウイルスRNAの分子量等からCymbidium mosaic virus(CyMV) と同定された。本ウイルスを12科57種の植物に汁液接種したところ、数種のラン科植物とゴマに全身感染した。また、本ウイルスは汁液接種で容易に伝搬されず、種子伝染も認められなかった。ツルナの病葉汁液中での安定性は不活化温度が65〜70℃(10分)、希釈限度が10-5〜10-6、保存限度が20℃で1〜2ヶ月であった。DN法による電顕観察では屈曲性のある長さ約475×13nmのひも状粒子が多数認められ、感染葉(Cymbidium) の超薄切片にはウイルス粒子がfingerprint状として細胞質内に存在することが確かめられた。ゲル電気泳動により外被タンパク質とウイルスRNAの分子量の解析を行ったところ、それぞれ27.8×103、2.2×106であった。また、本ウイルスに罹病したツルナ葉からは、主に4種の感染に特異的な二本鎖RNA(分子量約5.4×106、4.0×106、3.6×106 3.0×106)が検出された。 kn-abstract=A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. en-copyright= kn-copyright= en-aut-name=GaraI Wayan en-aut-sei=Gara en-aut-mei=I Wayan kn-aut-name=GaraI Wayan kn-aut-sei=Gara kn-aut-mei=I Wayan aut-affil-num=1 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=2 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=3 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Vanda orchid kn-keyword=Vanda orchid en-keyword=Cymbidium mosaic virus kn-keyword=Cymbidium mosaic virus en-keyword=Potexvirus kn-keyword=Potexvirus END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=1 article-no= start-page=9 end-page=20 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=数種禾穀類におけるアコニット酸の含量とアブラムシ密度との関係 kn-title=Further Observations on the Relationship between Aconitic Acid Contents and Aphid Densities on Some Cereal Plants en-subtitle= kn-subtitle= en-abstract=禾本科植物の葉に含まれているアコニット酸を抽出定量し、寄生性のアブラムシに対する耐性との関係を調べた。トウモコロシの16系統、典型的な抵抗性と感受性のソルガムの2系統、ヒエ類の3亜種、イネの2系統、オオムギの9系統とコムギの8系統について比較した。一般的に逆齢期の植物は老熟期の植物より高い含量であった。トウモコロシは全体として200μg/g以上を含有する系統が多く、アブラムシに対して耐性を示した。外部からトウモロコシアブラを導入し、そう推移とアコニット酸の関係を調べたところ、比較的耐性を示す系統は多量に含有し、アブラムが寄生し易い系統は少量であった。ヒエノアブラムシに耐性のソルガムの系統は感受性の系統の約2倍のアコニット酵を含量していた。タイヌビエのみがイヌビエ、ヒメタイヌビエ、イネのホウレイとアケボノと異なって、成熟するにつれてアコニット酸の含有量が増加していた。このことはトビイロウンカによって稲田が坪枯れの時にタイヌビエのみが青春としており、それはアコニット酸によるとの報告と一致している。オオムギは比較的少量のアコニット酸が検出され、耐性における作用は弱く、オオムギには特有のグラミンがあり、これがアブラムシに対する耐性の主要因に成っていると考えられた。コムギは少量のアコニットさん含有であり、グラミンは検出されなかった。数個の未同定の作用力不明の多量成分と摂取忌避作用の認められる微量のフェノール酸もあった。いくつかの成分が耐虫性成分であるが、アコニット酸も重要な要因と考えられた。 kn-abstract=Plant-feeding arthropods, in the course of evolution have adapted differentially to their plant host and thereby gained access to the available resource. Plants responded by developing chemicals and morphological defense to arthropods. Some secondary metabolic substances in plants have been assumed to be toxic or deter feeding. Aconitic acid in the barnyard-grass subspecies, Echinochloa oryzycola Vasing has been reported to be an active component against brown rice planthopper Nilapalvata lugens (Stal) (Kim et al. 1976). Aphids, Ropalosiphum padi. L., R. maidis (F.), Schizaphis graminum (Rondani)and Sitobion akeviae (Shinji), are important pests of barley (Hordeum vulgare L.) and wheat (Triticum aesstivum L.). On barley plants, more than seven aphids per stem causes yield loss (Hansen 1990). R. maidis sometimes severely infested corn plants (Zea mays L.), the leaves being wet by honeydew. Melanaphis sacchari (Zehntner) caused brownish decay of leaves of a susceptible line・ of sorghum Sorghum bicolor Moench. We examined the relation between aconitic acid contents and aphid densities in wheat, barley, corn, sorghum, barnyard grass and rice (Rustamani et al. 1992a). The plants containing larger amounts of aconitic acid showed a moderate degree of resistance to aphids. The findings obtained on additional lines in 1991, and on other components, such as DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), are reported herein. en-copyright= kn-copyright= en-aut-name=A.RustamaniMaqsood en-aut-sei=A.Rustamani en-aut-mei=Maqsood kn-aut-name=ルスタマニマクスード kn-aut-sei=ルスタマニ kn-aut-mei=マクスード aut-affil-num=1 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=3 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=79 end-page=88 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=オオムギの組換え型自殖系統、同質遺伝子系統、および倍加半数体系統を用いた発芽時における耐塩性の遺伝解析 kn-title=Genetical Studies on Salt Tolerance at Germination in Recombinant Inbred, Isogenic, and Doubled Haploid Lines of Barley (Hordeum vulgare L.) en-subtitle= kn-subtitle= en-abstract=オオムギの発芽時みおける耐塩性と形態マーカーとの連鎖関係を調べるために、Russia 6×HES 4の交雑から養成した組換え型自殖系統125系統と同質遺伝子系統70系統、およびLeger×CI 9831の交雑から養成した倍加半数体145系統の種子を1.5%の塩化ナトリウム溶液を用いて20℃、12時間日長の条件で発芽させ、置床後10日後に調査してシュートを25mm以上抽出した種子の置床種子に対する割合をもって発芽時の耐塩性とした。各種主働遺伝子が発芽時の耐塩性に及ぼす影響を解析するために、組換え型自殖系統、同質遺伝子系統、および倍加半数体系統を親品種間の間で異なる形質によって群別し、発芽時の耐塩性を比較した。その結果、Russia 6×HES 4の組換え型自殖系統、および同質遺伝子系統においては六条型は二条型よりも耐塩性が有意に強く、また、Leger×CI 9831の倍加半数体系統においては二条型は六条型よりも、底刺の短毛は長毛よりもそれぞれ耐塩性が有意に強く、条性間の耐塩性の強弱は交雑組合せによって逆転した。一方、芒の粗滑、穂密度、および頴色の比較においては有意差が認められなかった。以上の結果から、条性を支配するν遺伝子、および底刺のタイプを支配するs遺伝子と発芽時の耐塩性を支配する遺伝子との関連性が推察された。また、芒の粗滑を支配するr遺伝子、穂密度を支配するl遺伝子、および頴色を支配するRe-2遺伝子と発芽時の耐塩性は独立であると考えられた。 kn-abstract=To determine the relationship between morphological markers and salt tolerance at germination in barley (Hordeum vulgare L.), a total of 125 recombinant inbred (RI) lines of Russia 6×HES 4, a seriesof 70 isogenic (IG) lines for V/ν gene derived from Russia 6×HES 4, and 145 doubled haploid (DH) lines of Leger×CI 9831 were evaluated for their salt tolerance at germination. Comparison between each set of character pairs revealed that the six-rowed type was significantly more tolerant than the two-rowed type in the RI and IG lines of Russia 6×HES 4, annd the two-rowed type and the short haired rachilla type were significantly more tolerant than the six-rowed type and the long haired rachilla type in the DH lines of Leger×CI 9831. On the other hand, no significant difference was observed in salt tolerance such as the smoothness of awn, ear density, and lemma color, r (awn type), l (ear density), and Re-2 (lemma color) genes inherited independent of gene(s) for salt tolerance at germination. en-copyright= kn-copyright= en-aut-name=ManoYoshiro en-aut-sei=Mano en-aut-mei=Yoshiro kn-aut-name=間野吉郎 kn-aut-sei=間野 kn-aut-mei=吉郎 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Doubled haploid lines kn-keyword=Doubled haploid lines en-keyword=Hordeum vulgare kn-keyword=Hordeum vulgare en-keyword=Isogenic lines kn-keyword=Isogenic lines en-keyword=Recombinant inbred lines kn-keyword=Recombinant inbred lines en-keyword=Salt tolerancce kn-keyword=Salt tolerancce END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=137 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=オドントグロッサムリングスポットウイルスCy-1株RNAのゲノム構成および韓国株との比較 kn-title=Genomic Organization of Odontoglossum Ringspot Virus (Cy-1 Strain) RNA and Comparison with That of Korean Strain en-subtitle= kn-subtitle= en-abstract=オドントグロッサムリングスポットウイルスCy-1株(ORSV Cy-1)のcDNAクローンを用いて完全長ゲノムRNAの塩基配列を決定した。この配列の長さは6611塩基であり、126K、183K、31K、および18Kのタンパク質に対応する4個のオープンリーディングフレーム(ORF)を含んでいた。ORSV Cy-1の5'非翻訳領域は62塩基であった。126Kおよび、その183Kリードスル―産物をコードするORF内にはヘリカーゼとポリメラーゼの塩基配列モチーフが含まれていた。5'非翻訳領域である1から62塩基目の領域には2個のG残基とリボソーム結合領域(AUU)を保持していた。ORSV Cy-1の3'非翻訳領域は414塩基長であった。ORSV Cy-1の遺伝子構成は、韓国株(ORSV-K)とほとんど同じであった。しかしながら、ORSV Cy-1の183Kタンパク質をコードするORFは31Kタンパク質をコードしているORFと一部重なっていたが、ORSV−Kでは重なっていなかった。ORSV Cy-1およびORSV-Kの183Kタンパク質のアミノ酸数を比較すると、ORSV Cy-1とORSV-Kの塩基配列の相同性は96%であった。 kn-abstract=The complete nucleotide sequince of the genomic RNA of odontoglossum ringspot virus Cy-1 strain(ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K,183 K,31 K and 18 K proteins. The 5' non-coding region of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The5' non-coding region,which extends from bases 1 to 62 has 2G residues and the ribosome binding site (AUU). The3' non-coding region of ORSV Cy-1 composes 414 nucleotides in length. The genomic organization of ORSV Cy-1 is nearly identical to that of ORSV Korean strain(ORSV-K). However, the ORF encoding 183 K protein overlapes the ORF encoding 31 K protein in ORSV Cy-1, but not in ORSV-K. The 183 K read-through product of ORSV Cy-1 is 16 amino acids longer than that of ORSV-K. The homology of the nucleotide sequences of ORSV Cy-1 and ORSV-K is 96%. en-copyright= kn-copyright= en-aut-name=IkegamiMasato en-aut-sei=Ikegami en-aut-mei=Masato kn-aut-name=池上正人 kn-aut-sei=池上 kn-aut-mei=正人 aut-affil-num=1 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Tobamovirus kn-keyword=Tobamovirus en-keyword=Odontoglossum ringspot virus kn-keyword=Odontoglossum ringspot virus en-keyword=Nucleotide sequence kn-keyword=Nucleotide sequence en-keyword=Genome organization kn-keyword=Genome organization END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=103 end-page=110 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=大麦うどんこ病菌(Erysiphe graminis DC. f. sp. hordei)の地理的分化 kn-title=Geographic Differentiation of Barley Powdery Mildew Fungus(Erysiphe graminis DC. f. sp. hordei) en-subtitle= kn-subtitle= en-abstract=オオムギは世界的に広く栽培され、形態的・生理的特性を支配する遺伝子が地理的に規則性をもって分布している。そこで、本研究ではオオムギに罹病するオオムギうどんこ病菌の地理的分化を知るために、世界の異なる地域から採集した15菌系をオオムギ120品種に接種し、それらの反応を調査した。病原性は菌系によって、また、感受性はオオムギ品種によって著しく異なり、菌系と品種との相互作用が認められた。そこで、15菌系に対する120品種の反応を肉眼観察によって、8階級に分け、その値を主成分分析法によって解析した。第1主成分と第2主成分で全体の分散の55%以上を説明することが可能であり、これらの菌系は第1および第2主成分によって3群に分類することができた。供試した菌系についてみると、日本の3菌系のうち2株系は他のものと明らかに異なり、残りの1菌系はイスラエルや欧米の菌系と類似していた。さらに、欧米の菌系は2群に細分することができた。このことから、うどんこ病菌にも地理的な分化が生じているとみることができる。さらに、オオムギ品種をうどんこ病菌系に対する反応から分類してみると、東アジアの品種は欧州のものと明らかに異なり、オオムギの起源地である西南アジアの品種は極めて多様性に富んでいた。 kn-abstract=Fifteen cultures of barley powdery mildew fungus (Erysiphe graminis DC. f. sp. hordei) isolated in different regions of the world were inoculated to 120 barley varieties. Infection scores varied among the cultures and barley varieties, and their interactions were observed. Principal component analysis of the infection scores revealed that the cultures could be classified into three groups by first and second components (contributing 40.2% and 15.6% of the total variance, respectively), and that two Japanese cultures could be distinguished from the others isolated in Europe and North America. This indicates that the fungus is geographically differentiated in the reaction of the barley varieties to the cultures. Furthermore, East Asian barley varieties differed from European ones in their reactions to the culltures, while barley varieties from regions between East Asia and Europe showed a large genetic diversity in their reactions. en-copyright= kn-copyright= en-aut-name=KonishiTakeo en-aut-sei=Konishi en-aut-mei=Takeo kn-aut-name=小西猛郎 kn-aut-sei=小西 kn-aut-mei=猛郎 aut-affil-num=1 ORCID= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Powdery mildew kn-keyword=Powdery mildew en-keyword=Geographic distribution kn-keyword=Geographic distribution en-keyword=Differentiation kn-keyword=Differentiation END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=73 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=オオムギのアブラムシ抵抗性とグラミン:EDTA法による解析 kn-title=Gramine and Resistance of Barley to Aphids : Analysis of EDTA Exdates from Barley Leaves en-subtitle= kn-subtitle= en-abstract=オオムギのアブラムシ抵抗性要因物質の一つ、グラミンは幼苗に多量に存在している。アブラムシ口針切断法とEDTA法を用いて、アブラムシが吸汁するオオムギの師管液にグラミンが存在しているかどうか検討した。幼苗に寄生しているアブラムシの口針をレーザ光線を用いて切断したが、師管液は得られなかった。オオムギ葉を切断しEDTA溶液に浸し、切り口から液中に浸潤してくる成分は師管液に非常に類似したものといわれている。このEDTA法により得られた浸潤液のグラミンと糖の含量を時間を追って測定したところ、グラミンが師管液中に存在する可能性を示唆する結果を得た。 kn-abstract=Young leaves of barley contain a large amount of gramine, which is one of the factors involved in the resistance of barley against aphids. Using stylectomy by laser beam and EDTA-exudate method, we tried to determine if gramine exists in phloem sap which aphids ingest mainly. Phloem sap was not obtained by laser stylectomy using aphids feeding on young leaves. Components of exudates from cut leaves of barley in EDTA solution are known to be very similar to phloem sap. The time course of sucrose and gramine content of EDTA exudates from barley leaves suggested the existence of gramine in phloem sap. en-copyright= kn-copyright= en-aut-name=YoshidaHideya en-aut-sei=Yoshida en-aut-mei=Hideya kn-aut-name=吉田英哉 kn-aut-sei=吉田 kn-aut-mei=英哉 aut-affil-num=1 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=2 ORCID= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=3 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Resistance to aphids kn-keyword=Resistance to aphids en-keyword=Gramine kn-keyword=Gramine en-keyword=EDTA kn-keyword=EDTA en-keyword=Localization kn-keyword=Localization END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=4 article-no= start-page=269 end-page=277 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Detection of Leek Yellow Stripe Virus by Dot-immunobinding Assay kn-title=Leek yellow stripe virusのdot-immunobinding assayによる検出ならびにその診断への利用 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NodaChiyoichi en-aut-sei=Noda en-aut-mei=Chiyoichi kn-aut-name=野田千代一 kn-aut-sei=野田 kn-aut-mei=千代一 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=145 end-page=149 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cloning of PCR-Products Encoding Potassium Channel Proteins from Mesembryanthemum crystallinum kn-title=Mesembryanthemum crystallinum(ice plant)におけるカリウムチャンネルをコードするPCR産物のクローニング en-subtitle= kn-subtitle= en-abstract=耐塩性、耐乾性の極めて高い Mesembryanthemum crystallinum からPCR法を用いてカリウムチャンネル遺伝子断片を得た。2つのクローンが独立に得られたが、互いによく似ていて、シロイヌナズナのカリウムチャンネルとは67から88%の相同性を示した。サザンハイブリダイゼーションの結果から、今回得られた遺伝子はシングルコピーであり、またカリウムチャンネル遺伝子ファミリーが存在する可能性が示唆された。 kn-abstract=Gene fragments of potassium channels were cloned from Mesembryanthemum crystallinum by using RT-PCR (reverse transcription-polymerase chain reaction). The two fragments were isolated independently and showed high similarity with each other. About 80% identity was found between the two fragments and potassium-channel genes of Arabidopsis. Southern hybridization indicated that the potassium channel gene may be a single copy gene or that a small gene family of potassium channels exists. en-copyright= kn-copyright= en-aut-name=KatsuharaMaki en-aut-sei=Katsuhara en-aut-mei=Maki kn-aut-name=且原真木 kn-aut-sei=且原 kn-aut-mei=真木 aut-affil-num=1 ORCID= en-aut-name=BohnertHans J. en-aut-sei=Bohnert en-aut-mei=Hans J. kn-aut-name=BohnertHans J. kn-aut-sei=Bohnert kn-aut-mei=Hans J. aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Mesembryanthemum crystallinum kn-keyword=Mesembryanthemum crystallinum en-keyword=Potassium channel kn-keyword=Potassium channel en-keyword=RT-PCR kn-keyword=RT-PCR END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=145 end-page=153 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現 kn-title=Molecular Cloning,Sequencing and Expression of a cDNA Encoding Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Arabidopsis thaliana en-subtitle= kn-subtitle= en-abstract=シロイヌナズナから過酸化リン脂質グルタチオンペルオキシダーゼと高い相同性を持つタンパク質をコードするcDNAを単離し、その塩基配列を決定した。本遺伝子は、全長803bpからなり、169アミノ酸残基のタンパク質をコードしていた。アミノ酸配列は植物由来過酸化リン脂質グルタチオンペルオキシダーゼ様タンパク質と約80%の相同性を、哺乳類由来過酸化リン脂質グルタチオンペルオキシダーゼと約50%の相同性を示した。本遺伝子を大腸菌中で発現させた結果、遺伝子から予測される分子量をもつタンパク質が新たに生産された。 kn-abstract=A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one. en-copyright= kn-copyright= en-aut-name=SugimotoManabu en-aut-sei=Sugimoto en-aut-mei=Manabu kn-aut-name=杉本学 kn-aut-sei=杉本 kn-aut-mei=学 aut-affil-num=1 ORCID= en-aut-name=KawaiFusako en-aut-sei=Kawai en-aut-mei=Fusako kn-aut-name=河合富佐子 kn-aut-sei=河合 kn-aut-mei=富佐子 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Arabidopsis kn-keyword=Arabidopsis en-keyword=Phospholipid hydroperoxide glutathione peroxidase kn-keyword=Phospholipid hydroperoxide glutathione peroxidase en-keyword=Nucleotide sequence kn-keyword=Nucleotide sequence en-keyword=Gene expression kn-keyword=Gene expression END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=79 end-page=96 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ジゴキシゲニン標識プローブを用いたBeet Necrotic Yellow Vein Virus RNA の検出 kn-title=Nonradioactive Digoxigenin-labeled DNA Probes for the Detection of Five RNA Species Present in Beet Necrotic Yellow Vein Virus en-subtitle= kn-subtitle= en-abstract=テンサイそう根ウイルス(Beet nectotic yellow vien virus,BNYVV)に含まれる5種類のRNAのcDNAをクローニングした。得られたcDNAクローンのサイズは次の通りであった:RNA−1、3.0kbp;RNA−2、1.7kbp;RNA-3、1.8kbp;RNA−4、1.5kbp;RNA−5、1.4kbp。RNA-1とRNA-2由来のcDNAクローンは、それぞれ全ゲノムの3’末端側45%と35%を占めていた。RNA-3、RNA−4およびRNA−5からのcDNAクローンはほぼ全長であった。各cDNAクローンをジゴキシゲニン標識し、Northern blot hybridization でウイルスRNAの検出条件を検討した結果、試料はホルマリン・ホルムアルデミド変性、プローブのソニケーション、プローブ量は50ng/ml,ホルムアミド存在下の温度は42℃で行うのが最適であった。BNYVVの4〜5種のRNAを検出するためには、全RNA量として1〜10ngが適当であった。全RNA量が20ng以上では、RNA-3、RNA-4およびRNA-5の間でお互いにわずかに反応が認められた。この原因は、プローブの3’末端にみられる共通配列によることが確かめられた。Dot blot hybridization によるウイルスRNAの検出感度は、10pgであったが、試料の濃度が高くなるにつれて、RNA-3、RNA-4およびRNA-5の間でお互いに反応が認められた。各種BNYVV分離株を用いて、それらに存在するRNA組成とサイズをNorthern blot hybridization により解析したところ、検定植物ツルナ接種葉の場合には、直接全核酸を調整した試料からウイルスRNAの検出が可能であったが、Polymyxa betae 菌で接種されたテンサイの根の場合には、ウイルス濃度に応じて部分純化または濃縮した試料から核酸を調整する必要があった。このような条件下で、ほ場および室内分離株に存在するBNYVV RNA種およびそれらの欠失変異株を確実に検出することができた。 kn-abstract=Complementary DNA (cDNA) clones corresponding to each of five distinct RNA species of beet necrotic yellow vein (BNYVV) were synthesized and identified. The sizes of each cDNA clone for RNAs 1,2,3,4 and 5 molecules were 3.0, 1.7, 1.8, 1.5 and 1.4 kbp, respectively. cDNA inserts to RNA 2 were covered at a part of the 3'regions, and those of RNAs 3,4 and 5 were almost full-length. The plasmids containing each of cDNA inserts were labeled with digoxigenin by the random priming method. Northern blot hybridization tests showed that individual probes hybridized specially to each of the five RNAs. Good results were obtained with 1 to 10 ng of RNA as a mixture of five RANs, but the probe to RNA 3, RNA 4 or RNA 5 gave a weak signal with hererologous RNAs when more than 10 ng RNA was used. In dot blot hybridization, the limit of detection was about 10 pg RNA, but if a higher content of RNA was spotted, cross reaction occurred using heterologous RNAs. For laboratory and field isolates of BNYVV, each of RNAs 3,4 and 5 was easily detected by Northern blot hybridization in total nucleic acids extracted from Tetragonia expansa leaves inoculated mechanically, but not from roots of sugar-beet plants inoculated by the fungus Polymyxa betae. However, satisfactory results were obtained with partially purified or concentrated preparations from roots. These findings indicate that the digoxigenin-labeled probes are useful for the identification and detection of RNAs contained in field and laboratory isolates of BNYVV. en-copyright= kn-copyright= en-aut-name=SaitoMinako en-aut-sei=Saito en-aut-mei=Minako kn-aut-name=齊藤美奈子 kn-aut-sei=齊藤 kn-aut-mei=美奈子 aut-affil-num=1 ORCID= en-aut-name=KiguchiTadahiko en-aut-sei=Kiguchi en-aut-mei=Tadahiko kn-aut-name=木口忠彦 kn-aut-sei=木口 kn-aut-mei=忠彦 aut-affil-num=2 ORCID= en-aut-name=TamadaTetsuo en-aut-sei=Tamada en-aut-mei=Tetsuo kn-aut-name=玉田哲男 kn-aut-sei=玉田 kn-aut-mei=哲男 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Sugar beet kn-keyword=Sugar beet en-keyword=Rhizomania kn-keyword=Rhizomania en-keyword=BNYVV kn-keyword=BNYVV en-keyword=RNA detection kn-keyword=RNA detection en-keyword=Nonradioactive cDNA probe kn-keyword=Nonradioactive cDNA probe END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=123 end-page=134 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=カクヤリグサ科一年生雑草における数量分類学的研究 kn-title=Numerical Taxonomic Study in Annual Cyperaceous Weeds en-subtitle= kn-subtitle= en-abstract=カヤツリグサ科の3種の雑草カヤツリグサ(C.microiria Steud.)12系統、コゴメガヤツリ(Cyperus iria L.)12系統およびチャガヤツリ(C.amuricus Maxim.)6系統を岡山県、鳥取県および東京都なら採集して倉敷で栽培し、形態的特性、バイオマスおよび種子生産性など21形質を調査した。各形質を分散分析したところ系統間差はいずれも有意であったが、種間の値は多くの形質で重複し、単一形質による種の判別は困難であった。21形質間の相関行列を主成分分析したところ、上位3主成分の累積寄与率は83%と大きく、第1主成分(37%)は小穂の形態と種子生産性、第2主成分(28%)は生長量、第3主成分(18%)は種子重と小穂密度とそれぞれ関係が深いとみられた。第1主成分と第3主成分によって供試30系統は3群に分けられ、各群はそれぞれの種と一致した。さらに、第2主成分と第3主成分によってカヤツリグサ種内の系統は主として生長量の異なる3群に分類できた。クラスター分析によって供試30系統は4つの大きな群に分けられた。一番目の群には、C.amuricusの全系統、二番目の群にはC.microiriaの3系統、三番目の群にはC.microiriaの残りの系統、および最後のクラスターにはC.iriaの全系統が含まれた。従って、C.microiriaha形態的および生態的形質において異なる2ないし3の生態型から成ると考えられた。このように数量分類学あるいは多変量解析はカヤツリグサ科の雑草の種間ならびに種内の分類に極めて有効な解析法であることが示された。 kn-abstract=Three species of Cyperaceous weeds, Cyperus iria (12 strains), C. microiria(12 strains) and C. amuricus (6 strains), were collected from different sites of Okayama, Tottori and Tokyo prefectures, and various morphological characters, biomass and seed production were observed on the plants which were cultivated at Kurashiki. The analysis of variance showed a significant difference among the strains in each character. However, the species overlapped with each other in most morphological characters. Prinipal component analysis on the 21 characters showed that 83% of the total variation could be explained by the first three components: the first component (37%) was regarded as factors concerning spikelet and seed production; the second component (28%) was regarded as factors concerned the size of vegetative parts; the third component (18%) was largely affected by seed weight and floret density. Scatter diagram on the first and third principal components showed that the 30 strains of three species divided into three groups, and strains in each group correspond to the three species without exception. Based on the second and third principal components, strains of C. microiria were further divided into three sub-groups according to size of vegetative parts. Using the cluster analysis, 30 strains of these species were divided into four large clusters; the first was composed of C. amuricus strains, the second was of three strains of C. microiria, the third included the remaining strains of C. microiria, and the last cluster was composed of C. iria strains. It may be concluded that C. microiria is composed of two or three ecotypes which are different in morphological and reproductive traits. en-copyright= kn-copyright= en-aut-name=Muhamad AhmadChozin en-aut-sei=Muhamad Ahmad en-aut-mei=Chozin kn-aut-name=Muhamad AhmadChozin kn-aut-sei=Muhamad Ahmad kn-aut-mei=Chozin aut-affil-num=1 ORCID= en-aut-name=SatouKazuhiro en-aut-sei=Satou en-aut-mei=Kazuhiro kn-aut-name=佐藤和広 kn-aut-sei=佐藤 kn-aut-mei=和広 aut-affil-num=2 ORCID= en-aut-name=YasudaShozo en-aut-sei=Yasuda en-aut-mei=Shozo kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Cyperus iria kn-keyword=Cyperus iria en-keyword=Cyperus microiria kn-keyword=Cyperus microiria en-keyword=Cyperus amuricus kn-keyword=Cyperus amuricus en-keyword=Numerical taxonomy kn-keyword=Numerical taxonomy en-keyword=Speciation kn-keyword=Speciation END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=147 end-page=158 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=日本とカナダの春播オオムギから採取した大麦網斑病菌株の病原性の変異 kn-title=Pathogenic Variation of Pyrenophora teres Isolates Collected from Japanese and Canadian Spring Barley en-subtitle= kn-subtitle= en-abstract=北海道およびカナダの春播オオムギがら採取した22の大麦網斑病菌株(Pyrenophora teres Drechs.)を世界各地のオオムギ38品種に幼苗接種し、病斑指数によって病原性の変異を検討した。分散分析の結果、菌株の病原力ならびに品種の抵抗性には有意差が認められたが、菌株と品種の交互作用は統計的には有意でなかった。各菌株の反応をFinlay-Wilkinson(1963)の回帰分析によって解析したところ、北海道とカナダの菌株で病原性反応に差が認められた。この傾向はカナダの菌株のうち、通常のNet typeの菌株よりも斑点状病斑を示すSpot typeの菌株で顕著であった(Fig.1)。さらに、菌株と品種の交互作用を詳細に解析するために相互作用と相乗交互作用モデル(AMMIモデル)を適用して交互作用に関する主成分分析を行った結果、各菌株は日本のNet type、カナダのNet type、カナダのSpot typeの3群に分けられた(Fig.1)。各菌株について群間ならびに群内の相関係数を算出したところ(Table5)、Net typeの菌株相互の相関係数は0.601〜0.969と相対的に高かったが、一部の菌株と品種の組合わせでは抵抗性反応の逆転がみられた(Fig.4)。一方、Spot typeの菌株とNet typeの菌株の相関係数は0.302〜0.538と低く、両者の病原性は多少異なることが示された。このような一部の菌株と品種の間に認められる弱い交互作用は、抵抗性を支配する主働遺伝子の特異的な反応が、いわゆる圃場抵抗性を支配する微働遺伝子の作用によって修飾された結果と考えられる。 kn-abstract=Twenty-two isolates of Pyrenophora teres Drechs. collected from Japanese and Canadian spring barleys were inoculated to 38 barley varieties having various genetic backgrounds. The analysis of variance for the discase ratings showed that there were significant differences both in the virulence of isolates and the resistance of varieties. However, the interaction among isolates and varieties was not statistically significant. Both Finlay-Wilkinson regression analysis and principal component analysis by Additive Main effects and Multiplicative Interaction effects(AMMI)model classified the isolates into three groups,which were different in origins and sympton types. A spot tyte isolate was distinguished from net type isolates by its generally high virulence. A slight pathogenic differentiation was suggested between Japanese and Canadian net type isolates. en-copyright= kn-copyright= en-aut-name=SatoKazuhiro en-aut-sei=Sato en-aut-mei=Kazuhiro kn-aut-name=佐藤和広 kn-aut-sei=佐藤 kn-aut-mei=和広 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Hordeum vulgare kn-keyword=Hordeum vulgare en-keyword=Pyrenophora teres kn-keyword=Pyrenophora teres en-keyword=Barley kn-keyword=Barley en-keyword=Net blotch kn-keyword=Net blotch en-keyword=Race differentiation kn-keyword=Race differentiation END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=121 end-page=127 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ナメクジのα-グルコシダーゼの精製と性質 kn-title=Purification and Properties of α-Glucosidase from Slugs en-subtitle= kn-subtitle= en-abstract=ナメクジには多くのグリコシダーゼが存在し、摂取する植物多糖の代謝に関与している。その中で著量に存在し、澱粉代謝に関わっているα−グルコシダーゼについて研究を行った。ナメクジを破砕し、得られたα−グルコシダーゼを硫安分画、セファクリルS−200HRカラムクロマトグラフィー、CM−セルロースカラムトグラフィー、調製用ディスク電気泳動により精製した。本酵素はセファクリルS-200HRカラムトグラフィーで2種(T、U)に分画された。T画分はCM−セルロースクロマトグラフィーでさらに2種(T−1、T−2)に分画された。得られた3種のα−グルコシダーゼはマルトースやマルトオリゴ糖によく作用したが、イソマルトースに対する作用は弱かった。3種の中でも最も多量に存在するα−グルコシダーゼUは他の酵素(T−1、T−2)よりも可溶性澱粉に対する作用が顕著で、マルトースよりも強く作用した。 kn-abstract=Three forms of α-glucosidase(EC3.2.1.20), designated as T, U,V,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase V hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase T hyrolyzed soluble starch more slowly. en-copyright= kn-copyright= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=1 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Slug kn-keyword=Slug en-keyword=Incilaria bilineata kn-keyword=Incilaria bilineata en-keyword=α-glucosidase kn-keyword=α-glucosidase END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=159 end-page=166 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 kn-title=Purification and Properties of Wall-bound α-Glucosidase from Suspension-cultured Sugar-beet Cells en-subtitle= kn-subtitle= en-abstract=テンサイ培養細胞には数種のα‐グルコシダーゼが存在している。相当量のα‐グルコシダーゼ活性は細胞破壊後、緩衝液と食塩で抽出されるが、まだかなりのα‐グルコシダーゼ活性が細胞残渣に含まれたままである。その酵素は、界面活性剤やS-S結合切断試薬では遊離されなかったが、細胞壁分解酵素により可溶化された。テンサイ培養細胞にスミチームCとペクトリアーゼY-23を作用させると、細胞はプロトプラストになり、細胞壁由来の成分のみを分離できた。可溶化された部分からα‐グルコシダーゼを硫安分画、セファクリルS-200HRカラムクロマトグラフィー、CM-セルロースカラムクロマトグラフィーにより精製した。本酵素は、マルトース、ニゲロース、マルトオリゴ糖、可溶性澱粉に良く作用したが、それらに比べ、イソマルトースに対する作用は弱かった。本酵素は、マルトオリゴ糖と可溶性澱粉にマルトースよりも強く作用した。本酵素以外にテンサイ培養細胞とテンサイ種子から数種類のα‐グルコシダーゼが単離されているが、本酵素の基質的特異性はそれらのものと異なっていた。 kn-abstract=Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. en-copyright= kn-copyright= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=1 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Beta vulgaris L. cv. kn-keyword=Beta vulgaris L. cv. en-keyword=Tsukisappu kn-keyword=Tsukisappu en-keyword=Sugar-beet kn-keyword=Sugar-beet en-keyword=Wall-bound enzyme kn-keyword=Wall-bound enzyme en-keyword=α-Glucosidase kn-keyword=α-Glucosidase en-keyword=Protoplast kn-keyword=Protoplast END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=25 end-page=31 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=メダカハネカクシの尾部分分泌物 kn-title=Pygidial Secretion of Stenus Rove Beetles (Coleoptera : Staphilinidae) en-subtitle= kn-subtitle= en-abstract=ハネカクシ科の成虫は亜科や属によって特徴のある方法と特有の物質で防御分泌を行っている。メダカハネカクシ属の成虫は分泌器官を尾部に有し、エーテルに浸漬すると反転して肛門の両側に突出した。ホソフタホシメダカハネカクシをタバコ畑や野菜畑でスジクロメダカハネカクシ、コクロメダカハネカクシとトビイロメダカハネカクシを池、川やダムの水辺で採集した。採集虫はエーテルに約一分間浸漬して、分泌させた。分泌器官を観察し、分泌物と器官に残存している成分をガスクロマトグラフと質量分析計で調べた。ステナッシン、シネオール、イソピペリテノールと6-メチル-5ヘプテン-2-オンが同定できた。これらの構成成分が小動物や微生物に対して防御物質であり、水に落下した時の水表面の滑走に役だっていると考えられた。 kn-abstract=Rove beetles have a pair of pygidial excretory organs, each group secretes characteristic substances in a peculiar manner. The genus Stenus has a pair of pygidial organs, eversible by immersion in diethyl-ether at both sides of the anus. S. anthoracinus, S. melanarius vercecundus and S. rufescens were collected at ponds and river shores, and S. alienus was collected at tobacco and vegetable fields. Excretory substances were secreted in the diethylether, and identified by gas chromatography and mass spectrometry. Stenusin [N-Ethyl-3-(2-methylbutyl) piperidine], 1,8-cineole, isopiperitenol and 6-Me-5-hepten-2-one were identified. These may probably act as defensive substances against small attacking animals and microorganisms, and act as water suface moving active agents. en-copyright= kn-copyright= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Rove beetles kn-keyword=Rove beetles en-keyword=Stenus kn-keyword=Stenus en-keyword=Excretory secretion kn-keyword=Excretory secretion en-keyword=Pygidial organ kn-keyword=Pygidial organ en-keyword=Defensive secretion kn-keyword=Defensive secretion END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=23 end-page=30 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=2種のモノクローナル抗体を用いた簡易ELISAによるキュウリモザイクウイルス迅速・高感度検出 kn-title=Rapid and Sensitive Detection of Cucumber Mosaic Virus by a Simplified ELISA Using Two Monoclonal Antibodies en-subtitle= kn-subtitle= en-abstract=キュウリモザイクウイルス(CMV)に対するモノクローナル抗体(MAb)をコンジュゲートと試料を同時に反応させる簡易DAS-ELISAに適用したところ、同じMAbを捕捉抗体と検出抗体に使用した場合、ELISA値がかなり低くなり検出感度も低下した。一方、CMVの異なるエピトープ(抗原決定基)を認識する2種のMAbを用いた場合には、高感度な検出可能であったことから、この検出システムはCMVの迅速で高感度な検出法であることが示された。 kn-abstract=To detect cucumber mosaic virus (CMV), virus samples and conjugate were incubated together in a simplified double-antibody sandwich ELISA. The use of the same monoclonal antibody (MAb) as trapping (coating) and detecting antibodies resulted in considerable decrease of ELISA values and sensitivity due to the competition for antigen between trapping and detecting antibodies. The simplified ELISA using two MAbs which recognize different epitopes of CMV proved to be a rapid and sensitive method for virus detection. en-copyright= kn-copyright= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=1 ORCID= en-aut-name=SakoNobumichi en-aut-sei=Sako en-aut-mei=Nobumichi kn-aut-name=佐古宣道 kn-aut-sei=佐古 kn-aut-mei=宣道 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Cucumber mosaic virus kn-keyword=Cucumber mosaic virus en-keyword=Monoclonal antibody kn-keyword=Monoclonal antibody en-keyword=Simplified ELISA kn-keyword=Simplified ELISA END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=49 end-page=58 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=幼苗期と成熟期のオオムギ系統間における禾穀類アブラムシの密度とグラミン含量の関係 kn-title=Relationship between Gramine Concentration and Cereal Aphid Polulations in Seedling and Maturation Stages in Barley Lines en-subtitle= kn-subtitle= en-abstract=オオムギ(Hordeum spontaneum及びH.vulgare)14系統の幼苗期と成熟期における、アブラムシの密度とインドールアルカロイド化合物であるグラミン含量の関係を調べた。温室内の幼苗では、グラミン含量に関わらずムギミドリアブラムシ(Schizaphis graminum)の寄生密度に有意差は認められなかった。一方、ムギクビレアブラムシ(Rhopalosiphum padi)の密度は、幼苗間で異なる場合もあった。圃場におけるアブラムシの寄生に対して、抵抗性は出穂期に顕著に現れた。アブラムシの寄生密度とグラミン含量の間には負の相関が認められた。成熟期のオオムギでは、グラミンの分解活性の高い感受性系統と比較して、抵抗性系統のグラミン含量は多く、特に野生系統では著しかった。 kn-abstract=The relationship between the indole alkaloid gramine concentration and aphid population was examined seedling and maturation stages in 14 barley lines of Hordeum spontaneum and H. unlgare. The density of Schizaphis graminum did not differ significantly with the gramine concentration in the seedling in the greenhouse. However, the population of Rhopalosiphum padi sometimes differed with the seedling. The plant resistance to the natural infestation of cereal aphids was obvious at the heading stage. There was a negative correlation between the high population density of aphids and gramine concentration. The gramine concentration was high in matured resistant resistant lines, especially wild lines, as compared with susceptible lines due to higher biodegradation activity. en-copyright= kn-copyright= en-aut-name=MoharramipourSaeid en-aut-sei=Moharramipour en-aut-mei=Saeid kn-aut-name=MoharramipourSaeid kn-aut-sei=Moharramipour kn-aut-mei=Saeid aut-affil-num=1 ORCID= en-aut-name=MurataShin-ichi en-aut-sei=Murata en-aut-mei=Shin-ichi kn-aut-name=村田眞一 kn-aut-sei=村田 kn-aut-mei=眞一 aut-affil-num=2 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=3 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Resistance kn-keyword=Resistance en-keyword=Gramine kn-keyword=Gramine en-keyword=Cereal aphids kn-keyword=Cereal aphids END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=105 end-page=110 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=On the pectin-polygalacturonase of Saccharomyces fragilis. kn-title=Saccharomayces fragilisのペクチン―ポリガラクトウロナーゼについて en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OzawaJ. en-aut-sei=Ozawa en-aut-mei=J. kn-aut-name=小沢潤二郎 kn-aut-sei=小沢 kn-aut-mei=潤二郎 aut-affil-num=1 ORCID= en-aut-name=OkamotoA. en-aut-sei=Okamoto en-aut-mei=A. kn-aut-name=岡本賢一 kn-aut-sei=岡本 kn-aut-mei=賢一 aut-affil-num=2 ORCID= en-aut-name=HayashiT. en-aut-sei=Hayashi en-aut-mei=T. kn-aut-name=林哲吾 kn-aut-sei=林 kn-aut-mei=哲吾 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=1 article-no= start-page=25 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Multiple forms of endopolygalacturonase from Saccharomyces fragilis kn-title=Saccharomyces fragilisの多型のエンドポリガラクチュロナーゼ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=LimJ. en-aut-sei=Lim en-aut-mei=J. kn-aut-name=林栽允 kn-aut-sei=林 kn-aut-mei=栽允 aut-affil-num=1 ORCID= en-aut-name=YamasakiY. en-aut-sei=Yamasaki en-aut-mei=Y. kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=2 ORCID= en-aut-name=SuzukiY. en-aut-sei=Suzuki en-aut-mei=Y. kn-aut-name=鈴木幸雄 kn-aut-sei=鈴木 kn-aut-mei=幸雄 aut-affil-num=3 ORCID= en-aut-name=MinoA. en-aut-sei=Mino en-aut-mei=A. kn-aut-name=味野愛子 kn-aut-sei=味野 kn-aut-mei=愛子 aut-affil-num=4 ORCID= en-aut-name=OzawaJ. en-aut-sei=Ozawa en-aut-mei=J. kn-aut-name=小沢潤二郎 kn-aut-sei=小沢 kn-aut-mei=潤二郎 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= affil-num=5 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=43 end-page=53 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=大麦網斑病抵抗性の選抜効果 kn-title=Selection Effectiveness for the Resistance to Net Blotch in Barley en-subtitle= kn-subtitle= en-abstract=抵抗性と罹病性の両親間の交雑に由来する2組のF2集団とその後代のF3系統を用いて、大麦網斑病における抵抗性の選抜効果を推定した。F3系統の平均値と分散から、病斑指数の分散が小さく、すでに固定した系統が多数存在したので、抵抗性は少数の遺伝子に支配されているとみられた。F2個体とF3系統間の親子相関、親子回帰およびF2の選抜差とF3系統の遺伝獲得量から遺伝率を推定したところ、2組の交雑組合せで抵抗性親の病斑指数は異なっていたにもかかわらず、3種類の遺伝率の推定値はいずれも0.6〜0.8の値を示した。遺伝率が高く、しかも関与する遺伝子数であるため、雑種集団で連続変異を示す場合の大麦網斑病抵抗性の選抜はF2からでも効果的とみられた。 kn-abstract=Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy. en-copyright= kn-copyright= en-aut-name=SatoKazuhiro en-aut-sei=Sato en-aut-mei=Kazuhiro kn-aut-name=佐藤和広 kn-aut-sei=佐藤 kn-aut-mei=和広 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Net blotch kn-keyword=Net blotch en-keyword=Pyrenophora teres kn-keyword=Pyrenophora teres en-keyword=Selection kn-keyword=Selection en-keyword=Barley kn-keyword=Barley en-keyword=Disease resistance kn-keyword=Disease resistance END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=113 end-page=129 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=高対塩性オオムギ品種の選抜 kn-title=Selection for High Salt Tolerant Cultivars in Barley en-subtitle= kn-subtitle= en-abstract=耐塩性品種を選抜するため、500品種のオオムギを用い、水耕栽培実験を行った。培地の塩化ナトリウム濃度は2(対照区)、150および300mMとした。用いた品種500品種は、4581品種のオオムギから、発芽時の耐塩性の高い品種として選抜されたものである。1回に約100品種を用い、5回の水耕栽培実験で耐塩性の高い105品種を選抜した。この105品種を同一時期に水耕栽培し、主として塩化ナトリウム300mMでの地上部収量を比較することにより、最終選抜を行った。その結果、10余品種の高対塩性オオムギが得られた。これらの高対塩性オオムギ品種では、塩化ナトリウム150mMの場合に、対照区の42〜86%、300mMの場合に対照区の17〜30%の地上部収量が得られた。これらの実験に用いたオオムギ品種から、対塩性の高いもの、中程度のもの、および低いものを各々10数品種選び、それらのイオン含量を測定した。その結果、同一グループ内でもイオン含量にかなりの変動が見られるが、対塩性の高いグループでは、オオムギのナトリウム吸収に比較し、カリウム吸収の大きい傾向を認めた。 kn-abstract=In order to select higher salt tolerant cultivars of barley,solution culture experiments were carried out with 500 barley cultivars, which were preliminarily selected from 4581 cultivars by exposure to 1-2% of sodium chloride(MaCl) during the germinating stage. The concentrations of NaCl used in the experiments were 2 (control), 150 and 300 mM in nutrient solution.From the results of five solution culture experiments, each of which was carried out with about 100 cultivars, 105 cultivars of barley were selected as relatively about 100 cultivars. Then the final selection was carried out on those 105 cultivars, and it was found that more than 10 cultivars were highly salt tolerant, mainly based on the comparisons of shoot yields under the supply of 300 mM of NaCl.High salt tolerant barley cultivars mentioned above showed 42-86% and 17-30% of the control in shoot yield under the condition of 150 and 300 mM of NaCl, respectively.On three groups of barley,each having more than 10 cultivars, which are high, moderate and low in salt tolerance, ion contents of the plants were analyzed.The results suggest that potassium(K) uptake was comparatively larger in the high salt tolerant goup of barley cultivars,through there was a big variation in sodium(Na) and K contents even within the same group. en-copyright= kn-copyright= en-aut-name=SopandieDidy en-aut-sei=Sopandie en-aut-mei=Didy kn-aut-name=SopandieDidy kn-aut-sei=Sopandie kn-aut-mei=Didy aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= en-aut-name=MoritsuguMasumi en-aut-sei=Moritsugu en-aut-mei=Masumi kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=3 ORCID= en-aut-name=KawasakiToshio en-aut-sei=Kawasaki en-aut-mei=Toshio kn-aut-name=川ア利夫 kn-aut-sei=川ア kn-aut-mei=利夫 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Ion content kn-keyword=Ion content en-keyword=Salt tolerance kn-keyword=Salt tolerance en-keyword=Sodium chloride kn-keyword=Sodium chloride en-keyword=Yield kn-keyword=Yield END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue=1 article-no= start-page=43 end-page=51 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=1999 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エビネから分離されたインゲンマメ黄斑モザイクウイルスの諸性質 kn-title=Some Properties of Bean Yellow Mosaic Virus Isolated from Calanthe sp.(Orchidaceae) in Japan en-subtitle= kn-subtitle= en-abstract=山口県で採集した葉にモザイク症状を示すエビネからインゲンマメ黄斑モザイクウイルス(BYMV)が分離された。本ウイルスは汁液接種により試験した12科46種のうち9科29種に感染し、モモアカアブラムシにより非永続的に伝搬された。本ウイルスの粗汁液中での安定性、粒子の形態、管状封入体及び細胞内結晶状封入体の形態は既報のBYMVのそれらと同様であった。SDS-PAGEによる外被タンパク質の分子量は35,000であった。微沈降反応及びDAS-ELISAにおいて、本ウイルスはクロッカス及びグラジョラスから分離されたBYMVと血清学的に近縁であったが、グラジョラス分離株よりもクロッカス分離株の方が本ウイルスとより近縁であった。 kn-abstract=Bean yellow mosaic virus (BYMV) was isolated from Calanthe sp.showing mild chlorosis on the leaves, collected in Yamaguchi Prefecture in Japan. The virus was transmitted by the aphid Myzus persicae in a non-persistent manner, and by sap-inoculation to 29 out of 46 plant species from 9 out of 12 families tested. Stability in crude sap, morphology of virus particles, shape of cylidrical inclusions and the presence of cytoplasmic crystalline inclusions in the infected cells were similar to those of BYMV isolates previously reported. The virus contained a single protein species with a molecular weight of 35,000. In a microprecipition test and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the virus showed a close serological relationship to isolates of BYMV from both crocus and gladiolus, and showed a distant relationship to clover yellow vein virus. Three BYMV isolates used in this study were found to be serologically related to each other, but the virus was more closely related to the BYMV crocus isolate than to gladiolus isolate. en-copyright= kn-copyright= en-aut-name=MatsumotoJun-Ichi en-aut-sei=Matsumoto en-aut-mei=Jun-Ichi kn-aut-name=松本純一 kn-aut-sei=松本 kn-aut-mei=純一 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Calanthe sp. kn-keyword=Calanthe sp. en-keyword=bean yellow mosaic virus kn-keyword=bean yellow mosaic virus en-keyword=clover yellow vein virus kn-keyword=clover yellow vein virus en-keyword=serological relationship kn-keyword=serological relationship END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=51 end-page=57 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Note on the parasitic fungus, Spicaria rubido-purpurea AOKI, on the potato tuber moth, Gnorimoschema opercullela (ZELL). (Preliminary report) kn-title=Spicaria rubido-purpurea Aokiのジャガイモガに対する寄生性(予報) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OshimaS. en-aut-sei=Oshima en-aut-mei=S. kn-aut-name=大島俊市 kn-aut-sei=大島 kn-aut-mei=俊市 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=167 end-page=180 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ゼニゴケ葉緑体遺伝子と相同性を持つラン藻 Synechocystis PCC6803株のORF326、frxC およびORF469を標的にした変異の導入 kn-title=Targeted Mutagenesis of ORF326, frxC and ORF469 of a Cyanobacterium, Synechocysitis PCC6803, Homologous to Liverwort Chloroplast Genes en-subtitle= kn-subtitle= en-abstract=ゼニゴケ葉緑体ORF316、frxCおよびORFと相同性を持つ、形質転換型ラン藻Synechocystis PCC6803株のORF326、frxCおよびORF469の機能についての情報を得るため、これらの欠損株の作製を行った。コード領域にカナマイシン耐性遺伝子カセットを挿入することにより不活性化された変異型のORF(オーブンリーディングフレーム)を持つプラスミドを用いてSynechocystis PCC6803株の形質転換を行い、カナマイシンを含む培地で選抜した。Synechocystis PCC6803株は約10コピーのクロモソームを持つが、サザンブロット解析の結果、ORF326については、変異型と野生型のORF326の双方を持つ株しか得られず、増殖に必要と推測された。一方frxCおよびORF469については、ともに全て変異型に置き換わった株が得られ、増殖には必要ないことが示された。さらにORF469欠損株を光照射下で培養し、増殖速度とクロロフィルa濃度を測定したが、いずれも野生株とほぼ同じであり、ORF469は、この条件下では、増殖やクロロフィル生合成に必要ないと推察された。 kn-abstract=ORF326, frxC and ORF469 of a transfomable cynobacterium, Synechcystis PCC6803, have sequence similarity with ORF465 on the choroplast genome of a livewort, Marchantia polymorpha, respectively. To elucidate their functions,targeted mutagenesis was performed by transformation with clened DNA in which the ORF was disrupted by insertion of a kanamycin resistancen gene cassette.Streak-purifications of a single colony of each transformant were repeatde to segregate homozygous mutants for disrupted copies, because Synechocystis PCC6803 was reported to have approximately 10 chromosomal DNA copies. Southern blot analysis revealed that mutants for ORF326 had not only disrupted ORF326 copies but also wild type ORF326 copies. This suggests that ORF326 is indispensable for growth under the mixotrophic growth condition used. However, mutants for frxC and mutants for ORF469 had only mutated copies, indicating that they dispensable for growth. Growth and chlorophyll a content of an ORF469-disrupted mutant were compared and chlorophyll a content of an ORF469-disrupted mutant were compared to those of wild type under mixotrophic growth condition, but no significant difference was detected. This indicates that ORF469 is required for neither normal growth nor chlorophyll biosynthesis under thie condition. en-copyright= kn-copyright= en-aut-name=OguraYutaka en-aut-sei=Ogura en-aut-mei=Yutaka kn-aut-name=小倉豊 kn-aut-sei=小倉 kn-aut-mei=豊 aut-affil-num=1 ORCID= en-aut-name=TakemuraMiho en-aut-sei=Takemura en-aut-mei=Miho kn-aut-name=竹村美保 kn-aut-sei=竹村 kn-aut-mei=美保 aut-affil-num=2 ORCID= en-aut-name=OdaKenji en-aut-sei=Oda en-aut-mei=Kenji kn-aut-name=小田賢司 kn-aut-sei=小田 kn-aut-mei=賢司 aut-affil-num=3 ORCID= en-aut-name=OhyamaKanji en-aut-sei=Ohyama en-aut-mei=Kanji kn-aut-name=大山莞爾 kn-aut-sei=大山 kn-aut-mei=莞爾 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Cyanobacteria kn-keyword=Cyanobacteria en-keyword=Gene disruption kn-keyword=Gene disruption en-keyword=Synechocystis PCC6803 kn-keyword=Synechocystis PCC6803 END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=33 end-page=42 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=トウモロコシに含まれるDIMBOAとアブラムシ抵抗性の関係 kn-title=The Relationship between DIMBOA Concentration in Corn Lines and Resistance to Aphids en-subtitle= kn-subtitle= en-abstract=ハイドロキサム酸のDIMBOAは特定のイネ科植物にあり、寄生する昆虫や病原菌に対して抵抗性物質となっている。一方、トウモロコシにはトウモロコシアブラムシとムギクビレアブラムシが寄生し、養分奪取、排泄する甘露によるスス病や萎縮病ウイルスの伝播者としての害をもたらしている。トウモロコシの21系統を1990年と1991年に栽培し、生育に伴うDIMBOA含量の消長とアブラムシの寄生の変化を調べた。DIMBOAは単位体重当り若齢期に多量を含有し、生育に伴って少量になり、老齢期になると検出不可能の量となった。使用した系統は全て多少ともアブラムシに対して抵抗性を示し、オオムギやコムギなどで見られた増殖は見られなかった。大増殖している農家の圃場から移植したが、DIMBOA含量の多い若齢期においては急速に死滅した。DIMBOA含量の少ない成熟期から老齢期にかけては若干管生存した。ムギミドリアブラムシを用いて、人工飼育液にDIMBOA等を添加して生存率を調べて、他の作用物質との関連を考察した。トウモロコシには同じ篩管に含有されるアコニット酸も多量にあり、両物質が共に抵抗性物質であると考えられた。 kn-abstract=2,4-Dihydrox-7-methoxy1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid is considered to be one of the components responsible for the resistance to pest insects in cereal plants. The relationship between concentration of DIMBOA and aphid infestation on 21 corn lines was investigated in 1990 and 1991. DIMBOA was detected in leaves od all corn lines tested, contained larger amounts in young plants and gradually decreased with growth. This property was the same as observed in wheat. A more than ten times difference in DIMBOA concentration was observed in corn lines. However, there was no positive correlation between DIMBOA concentration and aphid density. Most of the lines used had resistance to aphids. Resistance may be built together with other components such as (E)-aconitic acid. en-copyright= kn-copyright= en-aut-name=RustamakiMaqsood A en-aut-sei=Rustamaki en-aut-mei=Maqsood A kn-aut-name=RustamakiMaqsood A kn-aut-sei=Rustamaki kn-aut-mei=Maqsood A aut-affil-num=1 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=3 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Apfid kn-keyword=Apfid en-keyword=Resistant substance kn-keyword=Resistant substance en-keyword=Hydroxamic acid kn-keyword=Hydroxamic acid en-keyword=DIMBOA kn-keyword=DIMBOA en-keyword=Corn kn-keyword=Corn END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=3 article-no= start-page=153 end-page=173 dt-received= dt-revised= dt-accepted= dt-pub-year=1966 dt-pub=196612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the weed control with trifluralin. I. Experiment on the weed control in rice paddy field. kn-title=Trifluralinによる雑草防除に関する研究 第1報 水稲田雑草防除試験 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KasaharaY. en-aut-sei=Kasahara en-aut-mei=Y. kn-aut-name=笠原安夫 kn-aut-sei=笠原 kn-aut-mei=安夫 aut-affil-num=1 ORCID= en-aut-name=NishiK. en-aut-sei=Nishi en-aut-mei=K. kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=2 ORCID= en-aut-name=UeyamaY. en-aut-sei=Ueyama en-aut-mei=Y. kn-aut-name=上山良人 kn-aut-sei=上山 kn-aut-mei=良人 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=2 article-no= start-page=51 end-page=60 dt-received= dt-revised= dt-accepted= dt-pub-year=1964 dt-pub=196403 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A virus disease of peanut caused by a strain of turnip mosaic virus. kn-title=Turnip mosaic virusの1系統によるナンキンマメの輪紋モザイク病 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=35 end-page=41 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 kn-title=Varietal Differences and Diallel Analysis of Pre-germination Flooding Tolerance in Soybean Seed en-subtitle= kn-subtitle= en-abstract=起源の異なる730品種の大豆種子を25℃、4日間浸漬処理し、その後の発芽率で種子の冠水抵抗性を評価した。また、種子の冠水抵抗性の遺伝様式6×6のダイアレル分析で調査した。供試した品種の冠水処理後の発芽率は0〜100%まで分布し、種子の冠水抵抗性には大きな変異が認められた。大部分の品種は冠水条件に感受性であり、供試品種の4%のみが高度の抵抗性(発芽率90%以上)を示した。ダイアレル分析の結果、種子の冠水抵抗性は、相加的効果と優性効果が有意であり、優性効果の方が相加的効果よりも重要であった。種子の冠水抵抗性を支配する遺伝子は2対、狭義の遺伝率は0.47と推定され、抵抗性の強い方が劣性なので、初期世代における選抜効果が期待できる。 kn-abstract=Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. en-copyright= kn-copyright= en-aut-name=HouFwu-Fenn en-aut-sei=Hou en-aut-mei=Fwu-Fenn kn-aut-name=侯福分 kn-aut-sei=侯 kn-aut-mei=福分 aut-affil-num=1 ORCID= en-aut-name=ThsengFu-Sheng en-aut-sei=Thseng en-aut-mei=Fu-Sheng kn-aut-name=曽富生 kn-aut-sei=曽 kn-aut-mei=富生 aut-affil-num=2 ORCID= en-aut-name=WuShu-Tu en-aut-sei=Wu en-aut-mei=Shu-Tu kn-aut-name=呉詩都 kn-aut-sei=呉 kn-aut-mei=詩都 aut-affil-num=3 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Glycine max kn-keyword=Glycine max en-keyword=Seed flooding tolerance kn-keyword=Seed flooding tolerance en-keyword=Diallel analysis kn-keyword=Diallel analysis en-keyword=Germination kn-keyword=Germination en-keyword=Varietal kn-keyword=Varietal en-keyword=difference kn-keyword=difference END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=1-2 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=196512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A virus disease of pea caused by white clover mosaic virus. kn-title=White clover mosaic virusによるエンドウのウイルス病 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=1 article-no= start-page=35 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Absorption of Iron by MuSty-Odor Producing Cyanobacteria (Anabaena macrospora, Phormidium tenue, Oscillatoria tenuis and Oscillatoria brevis) kn-title=かび臭物質産生ラン藻類Anabaena macrospora, Phormidium tenue, Oscillatoria tenuis 及びOscillatoria brevisの鉄吸収 en-subtitle= kn-subtitle= en-abstract=A recent problem for water utilities is musty-odor, which is caused by geosmin or 2-methylisoborneol produced by cyanobacteria. In order to clarify the mechanism of growth of musty-odor producing cyanobacteria, the effect of iron on the growth of Anabaena macrospora, Phormidium tenue, Oscillatoria tenuis and Oscillatoria brevis was studied. The growth of A. macrospora, P. tenue and O. tenuis was suppressed at low levels of iron (colloidal iron, 1 μM as Fe) in the EDTA-free CT medium. However, at high levels of iron (18 μM as Fe) using colloidal iron kept in a refrigerator, A. macrospora and P. tenue were able to grow although O. tenuis could not. When autoclaved colloidal iron was used as the iron source, none of the cyanobacteria except O. brevis were able to grow. The effect of spongy or powdery iron on the growth of cyanobacteria was also investigated. A. macrospora, P. tenue and O. brevis were capable of growing in media containing spongy or powdery ion, which release Fe(U) continuously, but the growth of O. tenuis was suppressed. The culture experiments and photoreduction of Fe(V) to Fe(U) show that A. macrospora, P. tenue and O. brevis can utilize iron even in the absence of EDTA if there are large amounts of iron present in a form photoreducible to Fe(U). However, O. tenuis was found to require chelated and photoreducible iron for its growth. The present results and previous studies {Nogaku Kenkyu, 62:253-270(1991)} indicate that O. brevis has the ability to utilize a wide variety of iron forms such as Fe(V)-EDTA, Fe(U)-BPDS, Fe(V)-Desferrioxamine B(microbial siderophore) chelates, colloidal iron, iron oxides (Fe2O3, Fe3O4), spongy iron and powdery iron. On the other hand, O. tenuis seems to have very specific requirements for the from of iron it can utilize for growth. kn-abstract=近年、琵琶湖の南湖をはじめ、霞々浦や相模湖など多くの湖沼や水道水源地において、人間活動に付随すると富栄養化問題となり、ラン藻類、特にかび臭物質を産生するラン藻の発生が憂慮されている。琵琶湖を水源とする大津、京都、大阪、神戸など関西の各都市の水道では、同湖で毎年発生するラン藻、すなわちAnabaena macrospora{ジオスミン(geosmin)を産生}やPhormidium tenue, Oscillatoria tenuis{2-メチルイソボルネオール(2-methylisoborneol, MIB)を産生}などが作り出すこれらのかび臭物質(ジオスミンまたはMIB)による水道水の異臭味が大きな問題となっている。こうした水圏環境における異臭味は世界各地でも問題となり、近年この問題に関する国際シンポジウム(International Symposium on Off-Flavors in the Aquatic Environment)が開かれるようになり、1982年6月には第1回(ヘルシンキ)、1987年10月には第2回(鹿児島)、そして1991年3月には第3回(ロサンゼルス)のシンポジウムがそれぞれ開催された。しかしこうしたラン藻が異常増殖する要因はまだ明らかではない。そのためかび臭物質を産生するラン藻類の発生を予測あるいは抑制する情報に関する研究は強い要請があるにもかかわらずあまり進展をみていない。 en-copyright= kn-copyright= en-aut-name=NakashimaSusumu en-aut-sei=Nakashima en-aut-mei=Susumu kn-aut-name=中島進 kn-aut-sei=中島 kn-aut-mei=進 aut-affil-num=1 ORCID= en-aut-name=AoyamaIsao en-aut-sei=Aoyama en-aut-mei=Isao kn-aut-name=青山勲 kn-aut-sei=青山 kn-aut-mei=勲 aut-affil-num=2 ORCID= en-aut-name=YagiMasakazu en-aut-sei=Yagi en-aut-mei=Masakazu kn-aut-name=八木正一 kn-aut-sei=八木 kn-aut-mei=正一 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=3 article-no= start-page=161 end-page=176 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=19760228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Chemical investigation on inorganic constituents in night soil (excreta) II. On the amounts of Ca, Mg, Na, K, P, Cl, S, Mn, Fe, Cd, Cu, Zn, Ni and Pb in the night soil of inhabitants of the Tohoku district kn-title=し尿中の無機成分に関する研究 (第2報) 東北地方のし尿中のCa, Mg, Na, K, P, Cl, S, Mn, Fe, Cd, Cu, Zn, Ni, Pbの含有量について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KobayashiJ. en-aut-sei=Kobayashi en-aut-mei=J. kn-aut-name=小林純 kn-aut-sei=小林 kn-aut-mei=純 aut-affil-num=1 ORCID= en-aut-name=MoriiF. en-aut-sei=Morii en-aut-mei=F. kn-aut-name=森井ふじ kn-aut-sei=森井 kn-aut-mei=ふじ aut-affil-num=2 ORCID= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=3 ORCID= en-aut-name=NakashimaS. en-aut-sei=Nakashima en-aut-mei=S. kn-aut-name=中島進 kn-aut-sei=中島 kn-aut-mei=進 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=1 article-no= start-page=1 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Turnip mosaic virus isolated from Iris (Studies on the viruses of bulbous ornamental plants in Japan. I) kn-title=アイリスから分離されたturnip mosaic virus (球根類花き植物のウイルスに関する研究 第1報) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=1 article-no= start-page=23 end-page=29 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=197103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=アブラムシの人工飼育(第1報) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=3 article-no= start-page=101 end-page=105 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=197203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=アブラムシの人工飼育(第2報)マメアブラムシの累代飼育 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=105 end-page=112 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Changes of Gramine Contents as an Aphid Resistant Substance with Barley Growth kn-title=アブラムシ抵抗性物質グラミンのオオムギの生育に伴う変化 en-subtitle= kn-subtitle= en-abstract=大麦はアブラムシ類の被害を受けることがある。ウイルス病の伝搬者として、また栄養分の掠奪者と組織細胞の破壊者として、害を及ぼしている。オオムギの系統の中にはアブラムシに対して、抵抗性の系統と感受性の系統がある。インドールアルカロイドであるグラミンはアブラムシを含む各種の動物に対して、毒性を有している。抵抗性と感受性の両者を含む27系統のオオムギについて、幼苗から成熟期迄のグラミン含量の変化を1989、1990、1991年の3年間にわたって調べた。オオムギは前年の11月20日頃播種し、翌年6月上旬に収穫した。全ての系統において幼苗は多量のグラミンを含有していた。抵抗性系統は感受性系統よりも多量に含有していた。この違いは、幼苗期においても成熟期におけるのと同じであった。全系統において、出穂前までの伸長生育期にグラミン含有は漸次減少した。4月初旬の減少し終わった時期に、アブラムシ類は飛来し、繁殖を始めた。4月下旬から5月上旬にアブラムシ類の密度は最高に達した。この時期に近づくに伴い抵抗性系統と感受性系統の明瞭な違いが見られ、グラミン含量の差も明瞭であった。グラミン含量は収穫まで余り変化せず、葉の老熟に伴う水分消失のため、単位重量当たりの換算値では多量になっていた。感受性系統は抵抗性系統に比べて、アブラムシ類が飛来し、繁殖する時期に、グラミンの分解が盛んで、少量のグラミンのみしか検出されなかった。分解に関与する機構が感受性系統では抵抗性系統よりも高いことが、系統間の差の大きな原因と推定された。 kn-abstract=Barley plants may be severely damaged by aphids,mainly because they may transmit viruses,suck food and disrupt tissues. There are resistant lines and susceptible lines in barley to aphids. The changing amounts of gramine from seeding to mature stages were investigated on 27 lines from 1989 to 1991. Barley was sown aound November 20 in the year and harvested in early June. Higher amount of gramine in seeding stage in all lines were detected,then gradually decreased with barley growth until 2 to 3 weeks before the earing stages. At this time aphids had appeared on the barley leaves and increased in population. The susceptible lines had a tendency of greater decrease in gramine content than the resistant lines at the time of aphid population growth. The maximum aphid population was observed from the end of April to early May. The gramine content was almost constant this time. The degradation mechanisms may be important factors determining the defference of resistant and susceptible lines at the aphid population growth stage. The susceptible lines may have a higher activity of degradation mechanism. en-copyright= kn-copyright= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=RustamaniMaqsood A. en-aut-sei=Rustamani en-aut-mei=Maqsood A. kn-aut-name=RustamaniMaqsood A. kn-aut-sei=Rustamani kn-aut-mei=Maqsood A. aut-affil-num=2 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=3 ORCID= en-aut-name=KasaharaToshihiko en-aut-sei=Kasahara en-aut-mei=Toshihiko kn-aut-name=笠原敏彦 kn-aut-sei=笠原 kn-aut-mei=敏彦 aut-affil-num=4 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Gramine content kn-keyword=Gramine content en-keyword=Barley kn-keyword=Barley en-keyword=Aphid kn-keyword=Aphid en-keyword=Resistant lines kn-keyword=Resistant lines en-keyword=Susceptible lines kn-keyword=Susceptible lines END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=17 end-page=26 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Quantitative Variations of a Resistance Substance, DIMBOA, against Aphids in Wheat Varieties. kn-title=アブラムシ抵抗性物質,DIMBOA, のコムギにおける消長 en-subtitle= kn-subtitle= en-abstract=コムギもオオムギと同様に系統・品種によってアブラシによる寄生の程度に差がみられた。数百のコムギ品種について、寄生程度の調査を1989年に予備的に行い、選別した8品種について、1990年と1991年にコムギの抵抗性物質とされているDIMBOAの消長を調べた。寄生程度の比較調査は1989年から1992年まで調べ、年と場所による変動を調べた。数千のオオムギの系統間においてはアブラムシ類の寄生数で百倍以上の差があり、抵抗性と感受性の系統を容易に選別できている。しかし、コムギにおいては数十倍の寄生数を示す系統は見出せなかった。系統間に年と場所での抵抗性と感受性の差が見られても、遺伝的であることが確認された系統はなかった。オオムギと比較して、供試した数百のコムギ系統の中には、アブラムシの寄生が非常に多い感受性系統はなく、供試した全てが多少とも抵抗性の形質を示した。コムギの抵抗性物質であるDIMBOAは冬期2月下旬で約1μg/g新鮮葉の含量であり、生育に伴って減少した。減少の程度は系統間で差が余りなかった。換言するとDIMBOAのない、あるいは極端に少量の系統はなく、全ての系統で抵抗性の一因となっていると考察された。コムギにおいては、オオムギで顕著な抵抗性物質と考えられているインドールアルカロイドのグラミンは検出されなかったが、オオムギで検出されなかったDIMBOAが顕著な抵抗性成分として検出され、これによって両ムギの質的な差異が示された。コムギはもう一つの抵抗性物質である水溶性のアコニット酸をオオムギの約10倍も含有しており、コムギではDIMBOAとアコニット酸の両者がアブラムシ抵抗性物質として作用していると考えられた。 kn-abstract=Aphids sometimes severely infest wheat plants, mainly sucking phloem sap and disrupting tissues, and in a few cases act as virus vectors. There are resistant and susceptible varieties of wheat against aphids. DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) is a substance causing resistance to animals including aphids. The change in the amounts of DIMBOA with growth in eight wheat varieties was estimated in 1990 and 1991. Wheat seeds were sown at mid-November in the former year and harvested in mid-June. Aphids appeared from early April, increased with the growth of the wheat, and finally decreased with the senescence of the wheat. Rhopalosiphum maidis appeared early in the season, R. padi appeared late, Schizaphis graminum and Sitovion akebiae appeared intermittently in the season. S. graminum appeared more frequently on wheat than barley. DIMBOA was detected from wheat but not from barley. Gramine (N,N-dimethyl-indole-3-methanamine) was detected from barley, and is known as an important resistance substance. However gramine could not be detected in wheat. DIMBOA was found in higher amounts in young wheat, and gradually decreased with growth. A clear relationship between the aphid population and DIMBOA amounts could not be observed. However, all the wheat varieties used in this experiment seemed to have resistance against aphids. The resistance was compared with barley susceptible lines. DIMBOA was presumed to share the property of resistance with aconitic acid in wheat. en-copyright= kn-copyright= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=Awan RustamaniMaqsood en-aut-sei=Awan Rustamani en-aut-mei=Maqsood kn-aut-name=Awan RustamaniMaqsood kn-aut-sei=Awan Rustamani kn-aut-mei=Maqsood aut-affil-num=2 ORCID= en-aut-name=ChengWen-Yi en-aut-sei=Cheng en-aut-mei=Wen-Yi kn-aut-name=鄭文儀 kn-aut-sei=鄭 kn-aut-mei=文儀 aut-affil-num=3 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=4 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=DIMBOA content kn-keyword=DIMBOA content en-keyword=Wheat kn-keyword=Wheat en-keyword=Aphid kn-keyword=Aphid en-keyword=Resistant varaity kn-keyword=Resistant varaity en-keyword=Susceptible variety kn-keyword=Susceptible variety END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=149 end-page=157 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Increase in Cytochorome c and a 11.9 kDa protein in Submerged Rice Seedlings after Exposure to Air kn-title=イネ水中芽生えの酸素適応過程におけるチトクロムcと11.9kDaタンパク質の増加 en-subtitle= kn-subtitle= en-abstract=イネ水中芽生えを空気に触れさせた後のチトクロムc含量の変動を調べるために、米ぬかから精製したチトクロムcを用いて抗チトクロムc血清を作成した。ウェスタンプロット分析によると、水中芽生えにはチトクロムcは検出されなかったが、空気に触れてから6時間以降に検出された。酸素適応24時間の芽生えには生重量あたりで好気対照と同レベルのチトクロムcが存在した。抗血清作成の抗原として用いたチトクロムc標品は、A408/A280比の値(4.66)が高いことから、よく精製されていると考えられるが、抗血清はいくつかの他のポリペプチドと反応した。そのうちの一つはチトクロムcよりも抗血清に強く反応し、その分子量は11.9kDaであった。このポリペプチドは酸素適応過程で増加し、水中芽生えや好気対照では24時間酸素適応芽生えよりも少なかった。 kn-abstract=To examine the changes in cytocrome c content in submerged rice seedlings after exposure to air, antiserum was prepared against purified cytocrome c from rice bran. Western blottong analysis revealed that cytochrome c was detected 6 h after exposure to air, but not detected in submerged rice seedling. On a fresh weight basis, the same level of cytochrome c as that of the aerobic control was found in the 24-h-air adapted seedlings. judging from the high A408/A280 ratio (4.66),the cytochrome c preparation used as antigen was considered to be well purified. However, the antiserum reacted other several polypeptides. One of them reacted more strongly against the antisermu than cytochrome c and its molecular weight was estimated as 11.9 kDa. The polypeptide increased during air adaptation and the levels found in both submerged seedlings and aerobic control were lower than that in 24-h-air-adapted seedlings. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=1 ORCID= en-aut-name=UshimaruTakashi en-aut-sei=Ushimaru en-aut-mei=Takashi kn-aut-name=丑丸敬史 kn-aut-sei=丑丸 kn-aut-mei=敬史 aut-affil-num=2 ORCID= en-aut-name=OokuboKatsuyuki en-aut-sei=Ookubo en-aut-mei=Katsuyuki kn-aut-name=大久保勝之 kn-aut-sei=大久保 kn-aut-mei=勝之 aut-affil-num=3 ORCID= en-aut-name=TsuchidaShin-ichi en-aut-sei=Tsuchida en-aut-mei=Shin-ichi kn-aut-name=土田進一 kn-aut-sei=土田 kn-aut-mei=進一 aut-affil-num=4 ORCID= en-aut-name=TsujiHideo en-aut-sei=Tsuji en-aut-mei=Hideo kn-aut-name=辻英夫 kn-aut-sei=辻 kn-aut-mei=英夫 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Air-adaptation kn-keyword=Air-adaptation en-keyword=Anti-cytochrome c serum kn-keyword=Anti-cytochrome c serum en-keyword=Cytochrome c kn-keyword=Cytochrome c en-keyword=Cytocrome c purification kn-keyword=Cytocrome c purification en-keyword=Oryza sativa kn-keyword=Oryza sativa END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=135 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Changes in Mitochondrial Spectra of Submerged Rice Seedlings after Exposure to Air Gaussian Deconvolution Analysis kn-title=イネ水中芽生えの酸素適応過程におけるミトコンドリアスペクトルの変化Gaussian Deconvolutionによる解析 en-subtitle= kn-subtitle= en-abstract=吸収スペクトルをカーブアナリシスの手法を用いて解析し、水中で発芽した芽生えが空気に触れたときに生じるミトコンドリアチトクロム類の量的変化を調べた。アンチマンイシンA存在下でのコハク酸還元による差スペクトルには3種のチトクロムbが見出されるが、チトクロムc1とよく似た成分がかなりの寄与で存在していることが判った。その寄与を除くといずれの芽生えから単離したミトコンドリアにおいても、3種のチトクロムbは互いにほとんど同程度の吸収を示した。また、水中芽生え、酸素適応芽生え、好気芽生えから単離したミトコンドリアの間には、3種いずれのチトクロムbの吸収(タンパク質当たり)に関しても差がなかった。チトクロムcは水中芽生えでは好気芽生えの約半分の量が存在し、酸素適応過程で1.5倍に増加した。これはチトクロムaa3の変化と全く同じであった。チトクロム類の中には、酸素適応過程において酸素に対する応答の異なる2つのグループが存在することを示した。 kn-abstract=Quantitative changes in mitochondrial cytochromes of submerged rice seedlings after exposure to air were investigated using gaussian deconvolution analysis on absorption spectrum at liquid nitrogen temperature. Differnece spectra were obtained from subtracting the absorption spectra of oxidized mitochondria from reduced mitochondria by succinate with antimycin-A. they showed three kinds of b-type cytochrome and a spectral component similar to cytochrome c1. Subtraction of the cytochrome c1-like component from the spectrum by curve analysis showed that three cytochrome bs had similar absorption maximum and that on a mitochondrial protein basis they were constant during air-adaptation. Cytochrome c per mitochontorial protein in submerged seedlings was about half of that in aerobic seedlings and increased by 1.5-fold during air-adaptation. The change in cytochrome c content was in paralleled with that of cytochrome aa3. These findings revealed that there were two groups of cytochromes in the response to air-adaptation,i.e.,three cytochrome bs were constant and cytochoromes c and aa3 increased in parallel. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=1 ORCID= en-aut-name=TsujiHideo en-aut-sei=Tsuji en-aut-mei=Hideo kn-aut-name=辻英夫 kn-aut-sei=辻 kn-aut-mei=英夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Absorption spectrum kn-keyword=Absorption spectrum en-keyword=Air-adaptation kn-keyword=Air-adaptation en-keyword=Cytocrome kn-keyword=Cytocrome en-keyword=Gaussian deconvolution analysis kn-keyword=Gaussian deconvolution analysis en-keyword=Oryza sativa kn-keyword=Oryza sativa END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue=1 article-no= start-page=1 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=1999 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Estimation of Turbulent Fluxes by Gradient Method with Surface Temperatures of Rice Plant Canopy kn-title=イネ群落表面温度を用いた傾度法による乱流輸送量の推定 en-subtitle= kn-subtitle= en-abstract=1997年夏季にイネ群落で、微細気象観測とともに、イネ群落の表面温度の測定を行った。得られた観測資料とイネ群落表面温度を用いてイネ群落上の乱流輸送量の推定を行った。結果は以下のように要約される。イネ群落上では、ほぼ全期間に亘って中立に近い安定な温度成層を形成し、日中には顕熱輸送量に比較して潜熱輸送量が格段に大きかった。熱映像温度計で測定されたイネ群落の表面温度から、群落上の顕熱輸送量と、植物の蒸散量と密接に関係する群落上の潜熱輸送量の推定を試みた。渦相関法で計算された顕熱輸送量とイネ群落表面温度から計算された顕熱輸送量と比較した。データのばらつきは大きいが、相関係数(R)の値は、0.79で、ほぼ1対1に対応していることが分かった。またイネ群落の表面で、水蒸気圧が飽和していると仮定し、イネ群落表面温度から、潜熱輸送量を推定すると、渦相関法で計算された潜熱輸送量よりも日中に2倍以上も過大評価されることが分かった。イネ群落直上で実測された水蒸気圧から、イネ群落表面の位置に相当する比湿を推定し、傾度法を用いて計算された潜熱輸送量が、渦相関法で得られた潜熱輸送量に、ほぼ1対1の対応をすることが分かった。また両者の間には、0.98と非常に高い相関関係が得られた。ここで行った計算には、尚幾つかの仮定が含まれているが、イネ群落の表面温度を用いて、顕熱、潜熱輸送量を推定する新たな評価法の一つとして今後利用できると期待される。 kn-abstract=Micrometeorological observations including turbulent fluxes and canopy surface temperatures were made in paddy in the summer of 1997. The sensible and latent heat fluxes were estimated by the gradient method under the assumption of similarity between the turbulent exchange coefficient for momentum and that for heat and water vapor. The surface temperatures measured by a thermal imager and water vapor pressures estimated from surface temperatures and relative humidity were used for temperature and water vapor at the height(d+z0) of U=0, where d is the displacement height, z0 the roughness length and U the mean wind velocity. The sensible and latent heat fluxes estimated by the gradient method depicted typical diurnal varietions. The turbulent fluxes by the present method and eddy correlation method showed fairly good agreement, especially for latent heat fluxes. These results confirmed that this method is a promising one. en-copyright= kn-copyright= en-aut-name=MiyashitaKoichi en-aut-sei=Miyashita en-aut-mei=Koichi kn-aut-name=宮下晃一 kn-aut-sei=宮下 kn-aut-mei=晃一 aut-affil-num=1 ORCID= en-aut-name=MaitaniToshihiko en-aut-sei=Maitani en-aut-mei=Toshihiko kn-aut-name=米谷俊彦 kn-aut-sei=米谷 kn-aut-mei=俊彦 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Surface temperature kn-keyword=Surface temperature en-keyword=Rice plant canopy kn-keyword=Rice plant canopy en-keyword=Thermal imager kn-keyword=Thermal imager en-keyword=Turbulent fluxes kn-keyword=Turbulent fluxes END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=117 end-page=127 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Differences in the Responses to Iron Deficiency Stress between Bean and Maize kn-title=インゲンとトウモロコシの鉄欠乏ストレスに対する反応の差異 en-subtitle= kn-subtitle= en-abstract=インゲンとトウモロコシを用い、鉄欠乏ストレスに対する両植物の反応の差異について検討した。その結果は以下のように要約できる。1)鉄欠乏ストレスに対して、インゲンの感受性は小さく、トウモロコシの感受性は大きかった。換言すれば、インゲンの鉄欠乏耐性は大きく、トウモロコシの鉄欠乏耐性は小さいということができる。2)鉄欠乏ストレスを受けたとき、インゲン根は鉄還元能と培地pH低下能を発現したが、トウモロコシ根ではそのような反応はほどんど認められなかった。3)鉄欠乏ストレスによるインゲン根での鉄還元能と培地pH低下能の発現は遮光処理、植物体地上部の切断処理、あるいは代謝阻害剤(DNP)の添加によって抑制された。4)鉄無添加処理はその後のインゲン植物体の鉄吸収を著しく促進した。トウモロコシではそのような現象は認められなかった。したがって、インゲンの鉄欠乏耐性が大きいのは、鉄欠乏ストレスによってインゲン根で鉄還元能と培地pH低下能が発現することによるものと考えられる。 kn-abstract=The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. en-copyright= kn-copyright= en-aut-name=YonetaniTsutomu en-aut-sei=Yonetani en-aut-mei=Tsutomu kn-aut-name=米谷力 kn-aut-sei=米谷 kn-aut-mei=力 aut-affil-num=1 ORCID= en-aut-name=MoritsuguMasumi en-aut-sei=Moritsugu en-aut-mei=Masumi kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=2 ORCID= en-aut-name=KawasakiToshio en-aut-sei=Kawasaki en-aut-mei=Toshio kn-aut-name=河ア利夫 kn-aut-sei=河ア kn-aut-mei=利夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Bean kn-keyword=Bean en-keyword=Iron deficiency stress kn-keyword=Iron deficiency stress en-keyword=Iron reducing capacity kn-keyword=Iron reducing capacity en-keyword=Maize kn-keyword=Maize en-keyword=Medium-pH lowering capacity kn-keyword=Medium-pH lowering capacity END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=137 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=1962 dt-pub=196203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the metabolism of uronic acids. 3. Properties of an intermediate in pectic acid metabolism by Erwinia aroideae: Products by ozone and periodate Oxidation. kn-title=ウロン酸の代謝に関する研究(3) Erwinia aroideaeによるペクチン酸の代謝中間生成物の性質―オゾン酸化と過沃素酸酸化による生成物 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OkamotoK. en-aut-sei=Okamoto en-aut-mei=K. kn-aut-name=岡本賢一 kn-aut-sei=岡本 kn-aut-mei=賢一 aut-affil-num=1 ORCID= en-aut-name=OzawaJ. en-aut-sei=Ozawa en-aut-mei=J. kn-aut-name=小沢潤二郎 kn-aut-sei=小沢 kn-aut-mei=潤二郎 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=1 article-no= start-page=39 end-page=47 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=196012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the metabolism of uronic acids. 2. Some properties of an intermediate compound in pectic acid metabolism by Erwinia aroideae. kn-title=ウロン酸の代謝に関する研究(2) Erwinia aroideae によるペクチン酸の代謝中間生成物の性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OkamotoK. en-aut-sei=Okamoto en-aut-mei=K. kn-aut-name=岡本賢一 kn-aut-sei=岡本 kn-aut-mei=賢一 aut-affil-num=1 ORCID= en-aut-name=OzawaJ. en-aut-sei=Ozawa en-aut-mei=J. kn-aut-name=小沢潤二郎 kn-aut-sei=小沢 kn-aut-mei=潤二郎 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=4 article-no= start-page=217 end-page=223 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Preventive effect of the proteinaceous inhibitor of ethylene synthesis on berry drop of Kyoho grape kn-title=エチレン生成阻害蛋白によるブドウ巨峰の脱粒防止の効果について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakaiS. en-aut-sei=Sakai en-aut-mei=S. kn-aut-name=酒井慎吾 kn-aut-sei=酒井 kn-aut-mei=慎吾 aut-affil-num=1 ORCID= en-aut-name=BabaI. en-aut-sei=Baba en-aut-mei=I. kn-aut-name=馬場赳 kn-aut-sei=馬場 kn-aut-mei=赳 aut-affil-num=2 ORCID= en-aut-name=OtaY. en-aut-sei=Ota en-aut-mei=Y. kn-aut-name=太田保夫 kn-aut-sei=太田 kn-aut-mei=保夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=3-4 article-no= start-page=197 end-page=202 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Preventive effect of the proteinaceous inhibitor of ethylene synthesis on berry drop of Kyohō grape (II) kn-title=エチレン生成阻害蛋白によるブドウ巨峰の脱粒防止の効果について(II) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakaiS. en-aut-sei=Sakai en-aut-mei=S. kn-aut-name=酒井慎吾 kn-aut-sei=酒井 kn-aut-mei=慎吾 aut-affil-num=1 ORCID= en-aut-name=BabaI. en-aut-sei=Baba en-aut-mei=I. kn-aut-name=馬場赳 kn-aut-sei=馬場 kn-aut-mei=赳 aut-affil-num=2 ORCID= en-aut-name=OtaY. en-aut-sei=Ota en-aut-mei=Y. kn-aut-name=太田保夫 kn-aut-sei=太田 kn-aut-mei=保夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=3-4 article-no= start-page=189 end-page=196 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Preventive effect of the proteinaceous inhibitor of ethylene synthesis on the injury caused by hormone-type herbicides in rice plant kn-title=エチレン生成阻害蛋白のホルモン型除草剤による水稲薬害の防止効果について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=BabaI. en-aut-sei=Baba en-aut-mei=I. kn-aut-name=馬場赳 kn-aut-sei=馬場 kn-aut-mei=赳 aut-affil-num=1 ORCID= en-aut-name=SakaiS. en-aut-sei=Sakai en-aut-mei=S. kn-aut-name=酒井慎吾 kn-aut-sei=酒井 kn-aut-mei=慎吾 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=163 end-page=174 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Odontoglossum Ringspot Virus Isolated from Calanthe sp. in Japan kn-title=エビネ(Calanthe sp.)から分離されたOdontoglossum Ringspot Virus en-subtitle= kn-subtitle= en-abstract=1992年に採集した退緑斑紋を示すエビネから棒状ウイルスとひも状ウイルスが検出され、この棒状ウイルスはその性質から odontoglossum ringspot virus(ORSV) と同定された。本ウイルスは汁液接種により供試した12科40種のうち、6科14種に感染した。DN法試料の電顕観察では長さ約310nmのウイルス粒子が観察された。本ウイルスの感染Chenopodium quinoa 細胞中には散在あるいは大集塊したウイルス粒子が観察された。ウイルス外被タンパク質の分子量は約20,600あり、Cymbidium からのORSV 2分離株とほぼ同じであったが、他の1つCymbidium からのCy-1 分離株は上記の分離株よりわずかに大きかった(約20,900)。本ウイルスと他のORSV分離株はいずれも寒天ゲル内二重拡散法、直接二重酵素結合抗体法(DAS-ELISA)でCy-1分離株の抗血清とよく反応したが、同じ抗血清に対してtabacco mosaic virus 普通系とは反応しなかった。ORSVエビネ分離株はウイルス感染に特異的な二本鎖RNAの電気泳動でも他のORSVと同様のパターンを示した。以上、ORSVエビネ分離株は既報のORSV分離株と血清学的に同じであり、それらと近似の系統と考えられた。 kn-abstract=A rod-shape virus isolated from Calanthe sp. showing chlorotic mottle on the leaves, collected in Ibaraki Prefecture, was identified as odontoglossum ringspot virus(ORSV). The isolate,designated as Cal.92-1T, was transmitted by sap-inoculation to 14 out of 40 species in 6 out of 12 families. The virus particles were rod-shaped, about 310 nm long. In ultrahtin sections, the dispersed and aggregated virus particles were observed in the cytoplasm of the cells of the infected leaves of Chenopodium quinoa. The virus contained a single protein species of Mr 20,600. The Mr of the capsid proteins(Cal.92-1T) was similar to those of three other ORSV isolates(Cy-1,Cy-46,Cy-Kei). Cal.92-1T isolate(Cy-1), suggesting that Cal.92-1T was serologically very similar to the other ORSV isolates. Three species of dsRNA were isolated from plants infected with Cal.92-1T and they were similar to those of three other ORSV isolates. en-copyright= kn-copyright= en-aut-name=MatsumotoJun-ichi en-aut-sei=Matsumoto en-aut-mei=Jun-ichi kn-aut-name=松本純一 kn-aut-sei=松本 kn-aut-mei=純一 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Odontoglossum ringspot virus kn-keyword=Odontoglossum ringspot virus en-keyword=Calanthe sp. kn-keyword=Calanthe sp. en-keyword=Identification kn-keyword=Identification END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=1 article-no= start-page=1 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=198211 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cucumber mosaic virus isolated from Calanthe discolor kn-title=エビネから分離されたcucumber mosaic virus en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MaedaT. en-aut-sei=Maeda en-aut-mei=T. kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=119 end-page=135 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Orchid Fleck Virus, the Causal Agent of a Yellowish Fleck Mosaic Disease of Calanthe kn-title=エビネ類に発生する黄色斑紋モザイク病の病原,Orchid Fleck Virusについて en-subtitle= kn-subtitle= en-abstract=1988年以来、山口・宮崎・鹿児島県で発生が認められたエビネ類(エビネ、タカネ、ヒゼン、サツマ、ツルラン、オナガエビネ)の葉に淡緑色〜黄色斑紋のモザイク病の病原ウイルスを調べたところ、orchid fleck virusと同定された。本ウイルスは11科46種(54品種)の植物に汁液接種を行ったところ、エビネに感染して原株と同様の病班を生じたほか、C.quinoa,フダンソウに全身感染し、C.murale,ホウレンソウ、ツルナ、ササゲ、タバコ、N.clevelandii,N.glutinosa,N.rusticaの5科12種の植物に局部感染が認められた。本ウイルスの粒子はDN法試料で被膜のない弾丸型であり、またときに短桿菌型も存在したが、その大きさは長さが約105〜125nm、幅約45〜50nmであった。病葉の粗汁液を用いた希釈限度は10-3〜10-4、不活性化温度は45〜50℃、保存限度は1〜2時間であった。ウイルスの汁液接種には、接種源植物としてC.quinoa またはツルナがよく、病葉磨砕後夏期では7分以内、冬期では15分以内の病汁液を供試すると接種検定によい結果が得られることが認められた。純化ウイルスを家兎に注射して、微滴法で力値512倍の抗血清が得られた。本抗血清はCymbidium から分離されたOFV・Cy-50とよく反応し、寒天ゲル内二重拡散法では Cal.94-16およびOFV・Cy-50の沈降帯が完全に融合した。ウイルスの構造蛋白質の分子量は約55Kであった。病細胞の超薄切片の電顕観察像には、閣内にviroplasmが認められ、その内部や周辺に層状に集塊あるいは散在した粒子が認められた。さらに核内や細胞質内に膜に包まれた車輪状の粒子集塊も見られた。OFVによる本病をエビネ類黄色斑紋モザイク病とした。 kn-abstract=Orchid fleck virus(OFV) was isolated from Calanthe spp.(Cal. discolor,Cal. Bicolor,Cal. Hizen,Cal. triplicata,Cal longicalcarata,Cal Satusma) showing light-green and/or yellowish fleck mosaic on the leaves, which different from previously known viruses of Calanthe. OFV caused systemic infection in Calanthe, Chenopodium quinoa and Beta vulgasis var. cicla, and local infection in C.amaranticolor, C. murale, Spinacia oleracea, Tetragonia expansa, Nicotiana tabacum, N. clevelandii, N. glutinasa, N. rustica, Vigna unguiculata. C quinoa and T expansa are useful as indecator hosts and as a source of virus for inoculation, diagnosis and purification. Sap from C. quinoa was infective after dilution to 10-3 but not 10-4, after 10 min at 45 but not 50℃, and after 1 hr at 20℃ but not 2 hrs. For sap inoculation, it is best to use the homogenate of OFV-onfected leaves within about 7-8 min after homogenization in summer and within about 15 min in winter. The virus particles were bullet-shape or bacilliform, approximately 45-50×105-125 nm in a negatively stained praparations. In ultrathin sections, the viroplasms were observed in the nuclei, and the virus particles and the chracteristic spokewheel structures were found both in the nuclei and the cytoplasm. Antiserum (precipitin tiner:1/512) against the present virus reacted strongly with the isolates of OFV-Cy-50, similar to that of homologous virus. In agar gel diffusion tests, no spur formation occurred among Cal. 94-16 and OFV-Cy-50. In SDS-polyacrylamide gel electrophoresis, one major band of Mr 55,000, probably viral nucleocapsid-protein, and three minor proteins were detected, similar to those of OFV・So from Cymbidium. en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MatsumotoJun-ichi en-aut-sei=Matsumoto en-aut-mei=Jun-ichi kn-aut-name=松本純一 kn-aut-sei=松本 kn-aut-mei=純一 aut-affil-num=2 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=3 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=5 ORCID= en-aut-name=TaharaMochimu en-aut-sei=Tahara en-aut-mei=Mochimu kn-aut-name=田原望武 kn-aut-sei=田原 kn-aut-mei=望武 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Calanthe kn-keyword=Calanthe en-keyword=Orchid fleck virus kn-keyword=Orchid fleck virus en-keyword=Calanthe yellowish fleck disease kn-keyword=Calanthe yellowish fleck disease END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=187 end-page=199 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Some Properties of Cymbidium Mosaic Virus Isolated from Calanthe spp. kn-title=エビネ(Calanthe spp.)から分離されたCymbidium mosaic virus en-subtitle= kn-subtitle= en-abstract=1986年、1990年および1993年に採集したモザイクを示すエビネからcymbidium mosaic virus(CyMV)を検出して同定し、諸性質を調べた。本ウイルスは汁液接種により供試した9科37種のうち、エビネとゴマに全身感染し、ツルナなど6科11種に局部感染した。しかし、分離株によってキュウリやセンニチコウの接種葉に感染するものと感染しないものがあった。ツルナ病葉粗汁液中での安定性は耐熱性65〜70℃、耐希釈性10-5〜10-6、耐保存性1〜2ヶ月であった。DN法試料の電顕観察では長さ約475nmのウイルス粒子が観察された。ウイルス外被タンパク質の分子量は約27,800であり、既報のCyMVの値とほぼ同じであった。ツルナ感染葉中の感染に特異的な二本鎖RNAの電気泳動ではreplicative form と思われる4.43×106と3.84×106のバンドが認められた。エビネから分離されたCyMV分離株は直接二重酵素結合抗体法(DAS−ELISA)で、Cy−16分離株の抗血清とよく反応するものが多かったが、反応が弱く、抗原性に若干の違いがあるものが存在した。 kn-abstract=Cymbidium mosaic virus(CyMV) was isolated from Calanthe spp. showing mosaic on the leaves, collected in Yamaguchi and Kyoto Prefectures in 1986〜1993. CyMV, Cal. 90-1 isolate was transmitted by sapinoculation to 12 out of 37 species in 7 out of 9 families. Sap from diseaded Tetragonia expansa was infective to Chenopodium amaranticolor after dilution to 10-5 but not 10-6, after heating at 65℃ for 10 min but not 70℃, and after 1 month at 20℃ but not 2 months. The virus particles were flexuous rod, about 475 nm long. The virus was purified from diseased T. expansa leaves and contained a single protein species of Mr27,800. The Mr of the capsid proteins(Cal. 90-1) was similar to those of two ohter CyMV isolates(Cal. 90-4, Cal. 93-14).Cal. 90-1 and Cal. 93-14 reacted with antiserum to the Cymbidium isolate (Cy-16), suggesting that Cal. 90-1 was serologically very similar to the other two CyMV isolates. Two species of dsRNA were isolated from plants infected with Cal-1 and they were similar to those of two other CyMv isolates. en-copyright= kn-copyright= en-aut-name=MatsumotoJun-ichi en-aut-sei=Matsumoto en-aut-mei=Jun-ichi kn-aut-name=松本純一 kn-aut-sei=松本 kn-aut-mei=純一 aut-affil-num=1 ORCID= en-aut-name=UrabeShinji en-aut-sei=Urabe en-aut-mei=Shinji kn-aut-name=占部慎治 kn-aut-sei=占部 kn-aut-mei=慎治 aut-affil-num=2 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=3 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=5 ORCID= en-aut-name=TaharaMochimu en-aut-sei=Tahara en-aut-mei=Mochimu kn-aut-name=田原望武 kn-aut-sei=田原 kn-aut-mei=望武 aut-affil-num=6 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=岡山大学 en-keyword=Calanthe spp. kn-keyword=Calanthe spp. en-keyword=Cymbidium mosaic virus kn-keyword=Cymbidium mosaic virus en-keyword=Potexvirus kn-keyword=Potexvirus END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=1 article-no= start-page=33 end-page=44 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Lettuce mosaic virus isolated from pea (Pisum sativum) kn-title=エンドウから分離されたLettuce mosaic virusについて en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KuidaK. en-aut-sei=Kuida en-aut-mei=K. kn-aut-name=杭田要 kn-aut-sei=杭田 kn-aut-mei=要 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=4 article-no= start-page=181 end-page=186 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=198503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effect of population density on the wing development of the pea aphid, Acyrthosiphon pisum kn-title=エンドウヒゲナガアブラムシの有翅型出現に及ぼす飼育密度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsujiH. en-aut-sei=Tsuji en-aut-mei=H. kn-aut-name=辻博夫 kn-aut-sei=辻 kn-aut-mei=博夫 aut-affil-num=1 ORCID= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=4 article-no= start-page=221 end-page=226 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Propagation of the Pea Aphid, Acyrthosiphon pisum kn-title=エンドウヒゲナガアブラムシの有翅胎生雌と無翅胎生雌の発育と増殖に及ぼす温度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsujiHiroo en-aut-sei=Tsuji en-aut-mei=Hiroo kn-aut-name=辻博夫 kn-aut-sei=辻 kn-aut-mei=博夫 aut-affil-num=1 ORCID= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=4 article-no= start-page=189 end-page=195 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=197101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=エンドウ・種子伝染モザイク・ウィルス感染細胞にみられる細胞質内封入体 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=81 end-page=86 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Influence of temperature on the development of Telmatoscopus albipunctatus Williston kn-title=オオチョウバエの発育に及ぼす温度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MoriharaO. en-aut-sei=Morihara en-aut-mei=O. kn-aut-name=森原修 kn-aut-sei=森原 kn-aut-mei=修 aut-affil-num=1 ORCID= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=1 article-no= start-page=51 end-page=59 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Hybrid Progenies Virulent to Wheat and Agropyron Derived from a Cross Between Barley Mildew Isolates (Erysiphe graminis DC. f. sp. hordei) kn-title=オオムギうどんこ病(Erysiphe graminis DC. f. sp. hordei)菌株間の雑種から育成したコムギおよびカモジグサに病原性のある菌株 en-subtitle= kn-subtitle= en-abstract=Repeated selection and hybridization among progeny isolates derived from a cross between two strains of barley mildew originally avirulent to wheat and Agropyron has given rise to isolates virulent on those plants. Barley mildew isolates h9 and Hna were hybridized on a susceptible barley host Kuromugi 148 to produce perithecia. After meiosis a number of haploid ascospores developed in the diploid perithecium. The resulting ascospores were inoculated onto Kuromugi 148 to develop conidiospores. Conidiospores sampled from 100 disease lesions were separately inoculated onto 100 seedligs of Kuromugi 148 and single conidiospores collected from each seedling were individually inoculated onto 100 seedlings of Kuromugi 148 to establish 100 progeny isolates. For evaluating pathogenicity of the isolates, conidiospores sampled from each isolate were inoculated onto 100 seedlings each of wheat cultivars Norin 52 and Norin 61, Agropyron Ag. E. e. and Ag. S. 4. The most virulent among the 100 isolates were selected and hybridized again on Kuromugi 148 to produce the next generation. This cycle of hybridization and selection was repeated four times. In the first generation, which was not subjected to selection, most of the isolates showed no or very weak growth on the non-host wheat and Agropyron plants. In the second generation which was derived from hybridization between the most virulent isolates of the first generation, some of the progenies showed weak virulence on the whear or Agropyron plants. In the succeeding generations the degree of virulence on the wheat or Agropyron plants increased gradually in response to selection. After four cycles of hybridization and selection some isolates developed highly virulent symptoms on the originally resistant wheat and Agropyron non-host plants. This increased virulence on wheat and Agropyron plants may be due to the recombination and segregation of minor genes promoting virulence derived from the parents h9 and Hna which were originally avirulent on both wheat and Agropyron. Our results indicate that new virulent mildew isolates may develop in nature after hybridization between two avirulent isolates. kn-abstract=イネ科植物うどんこ病菌(Erysiphe graminis DC.)はコムギ、オオムギ、カモジグサ、ライムギなど多くのイネ科植物に寄生するが、それぞれの菌は宿主特異性によっていくつかのformae specialesに分化しており、例えば、オオムギうどんこ病菌はオオムギ属以外の植物には感染しないといわている。しかしながら、Cherewickはイネ科植物うどんこ病菌について、元々の宿主とは異なる植物に接触する交互接種を試み、カモジグから分離したうどんこ病菌がコムギを犯す事例を見出した。またHardisonはいろいろなイネ科植物から分離したうどんこ病菌を用いて多くの交互接種を行った結果、ほとんどの菌株は2つあるいはそれ以上の属の植物に病原性を持っていることを認めた。さらに、日浦および日浦と部田はコムギうどんこ病菌とカモジグサうどんこ病菌の種間交雑によって得られた後代から、コムギあるいはカモジグサのいずれかに病原性を持つ菌株はもちろん、コムギとカモジグサの両方に病原性を持つ新しい菌株も容易に作れることを報告した。一方、これらの分化型の中にはそれぞれの宿主の品種あるいは系統に対して病原性の異なる多くのphysiologic raceが分化しており、それらの宿主-病原関係には、いわゆるgene for gene仮設が適合すると考えられている。しかしながら、部田はオオムギうどんこ病菌抵抗性遺伝子JMlsnに対して病原性のない菌株h9とH14の交雑後代からJMlsnに病原性のある菌株を作出した。これらの事実はうどんこ病における宿主-病原菌の相互作用が単純な主働遺伝子によるgene for gene仮設では説明し切れないことを示しており、このような非病原性菌株間の交雑後代から病原性のある菌株が脈生する遺伝的な機構を解明することは、うどんこ病抵抗性育種を進める上でも極めて重要であると思われる。 en-copyright= kn-copyright= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=4 article-no= start-page=279 end-page=286 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Production of Virulent Hybrid Progenies to Hordeum spp. with Resistant Gene JMlsn by Crossing between Two Avirulent Isolates of Erysiphe graminis DC. f. sp. hordei kn-title=オオムギうどんこ病抵抗性遺伝子JMlsnに対する非病原性菌株間の交雑による病原性菌糸の作出 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=11 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Reaction of the Resistant Gene ml-o to Various Barley Powdery Mildew Strains kn-title=オオムギうどんこ病抵抗性遺伝子ml-oの各種菌株に対する反応 en-subtitle= kn-subtitle= en-abstract=うどんこ病抵抗性遺伝子ml-oを持つ7つの突然変異系統と原品種に対して圃場で採取したうどんこ病菌4菌株と交雑由来の4菌株計8菌株を接種し、ml-oの突然変異系統とその原品種のうどんこ病菌に対する反応を比較した。供試8菌株は判別品種に対して相互に異なる反応を示し、従って異なる病原性遺伝子を持つものとみられた。一方、7つの原品種は8つの菌株に対する反応が一般的に良く似ており、抵抗性に関する遺伝的背景が似通っているとみられた。ml-oを持つ突然変異体とその原品種を比較すると、一部の例外を除けば病斑型はほとんど等しい一方、病斑数が顕著に減少していたので、ml-oは病斑型に対してではなく、病斑数を減少させる方向に働くとみられる。 kn-abstract=Eight powdery mildew strains, four collected from the field and the other four derived from a cross between two strains, were inoculated to seven mutant barley lines having the ml-o gene for powdery mildew resistance and their original varieties of barley. These eight powdery mildew strains showed a distinctive reaction to the nine testers having various resistant genes. Onthe other hand, the original barley varieties showed a similar reaction to all the powdery mildew strains inoculated, indicating that the genetic background of these varieties was similar. In comparison with the ml-o mutants and their original varieties, the type of lesion did not change in most cases, but the number of lesions decreased significantly without exception. In conclusion, the powdery mildew resistant gene ml-o does not affect the type of lesions but it reduces the number of lesions. en-copyright= kn-copyright= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Powdery mildew resistance kn-keyword=Powdery mildew resistance en-keyword=Barley kn-keyword=Barley en-keyword=ml-o gene kn-keyword=ml-o gene en-keyword=Pathogenicity kn-keyword=Pathogenicity END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=69 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=QTL Mapping for Powdery Mildew (Erysiphe graminis DC. f. sp. hordei EM Marchal) Resistance in Barley (Hordeum vulgare L.) kn-title=オオムギうどんこ病抵抗性遺伝子のQTLマッピング en-subtitle= kn-subtitle= en-abstract=オオムギ品種Steptoe×Morex (S/M)およびHarrington×TR306 (H/T)の2交雑組合せからバルボッサム法によって育成された倍加半数体(DH)系統を用い、オオムギうどんこ病菌h81、h83(S/M)、h9、h4およびk6(H/T)に対する抵抗性を調べ、抵抗性のデータとマーカー情報からオオムギうどんこ病抵抗性を支配する遺伝子の位置と効果をQTLマッピングにより推定した。その結果、S/Mでは各菌株に対して特異的に発現するいくつかの抵抗性遺伝子が4H、5H、6H、および7Hに見出された。一方、H/Tにおいてはいずれの菌株に対してもDH系統群の抵抗性スコアの分離比が抵抗性:罹病性=1:1に適合し、見出された遺伝子は抵抗性遺伝子Mlgと同一あるいは同座であるとみられた。 kn-abstract=Powdery mildew, caused by Erysiphe graminis DC. f. sp. hordei EM Marchal, is a serious disease of Barley (Hordeum vulgare L.). In this study, we used molecular markers to identify the chromosomal locations carrying genes for powdery mildew resistance and to estimate the effect of each gene. Doubled haploid lines derived from Steptoe×Morex (S/M), Harrington×TR306 (H/T) and their parental were inoculated with five powdery mildew strains. Several quantitative trait loci (QTL) controlling E. graminis resistance were found and lacated on chromosome 4H, 5H, 6H and 7H in S/M. On the other hand, no QTL was detected in H/T but Harrington had a major resistant gene (Mlg) for powdery mildew resistance. Maker-assisted selection was conducted to examine the effect of accumulation for mildew resistance. There was a significant interaction between QTLs lacated in 4H and 7H, which suggested the presence of an epistatic effect between these QTLs. en-copyright= kn-copyright= en-aut-name=IwasaTomohiko en-aut-sei=Iwasa en-aut-mei=Tomohiko kn-aut-name=岩佐友彦 kn-aut-sei=岩佐 kn-aut-mei=友彦 aut-affil-num=1 ORCID= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Hordeum vulgare kn-keyword=Hordeum vulgare en-keyword=Erysiphe graminis kn-keyword=Erysiphe graminis en-keyword=QTL mapping kn-keyword=QTL mapping en-keyword=DH lines kn-keyword=DH lines END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=3 article-no= start-page=113 end-page=124 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of two kinds of very early genes on yield and its components in barley kn-title=オオムギにおける2種の極早生遺伝子の収量とその構成要素に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YasudaS. en-aut-sei=Yasuda en-aut-mei=S. kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=1 ORCID= en-aut-name=HayashiJ. en-aut-sei=Hayashi en-aut-mei=J. kn-aut-name=林二郎 kn-aut-sei=林 kn-aut-mei=二郎 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=69 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Character Expression and Inheritance of a "Short Upper Leaves" Mutant in Barley kn-title=オオムギにおける上位葉短縮型変異体の形質表現と遺伝性 en-subtitle= kn-subtitle= en-abstract=二条オオムギ品種ふじ二条にガンマー線を3代にわたって合計90KR累代照射した後代から上位の数葉が短縮し、特に止葉は痕跡的に短くなる変異体を見出し、その形質表現と遺伝性を解析した。1)この変異体では基部の数枚の葉身の長さは原品種と変わらず、上位の数葉、特に止葉が強く短縮する。穂長および小穂段数は原品種と変わらない。2)この変異体は不完全優性の主働遺伝子Sul(short upper leaves上位葉短縮型)に支配される。3)Sul遺伝子は第2染色体に座乗するν/V(条性)遺伝子と連鎖し、また相互転座点と密接に連鎖している。4)この変異体は登熟期における葉面積が小さいので相互遮蔽が少なく、また収穫指数が高まって多肥密植栽培で多収となる可能性があり、また、蒸散量が少なく、半乾燥条件に適応することが期待される。 kn-abstract=A mutant which devolops an extremely shortened flag leaf and shorter upper leaves was obtained from the progeny of 90 KR of gamma-ray irradiated Fuji Nijo, a malting barley variety. This mutant was controlled by a single partial dominant gene named Sul (short upper leaves). The character expression and the inheritance of the gene were investigated in this study. Sul shortened the flag leaf and upper several leaves and uppermost internode length, while it did not affect the length of spike, lower leaves and the second or lower internodes. A reciprocal translocation of the chromosomes was observed in this mutant line, and the Sull gene was linked with the V (two-rowed) gene on chromosome 2, and also linked with the breaking point of the reciprocal translocation. Small leaf area caused by the Sull gene might reduce the mutual shading and the transpiration of the leaf canopy at the later growth stages. en-copyright= kn-copyright= en-aut-name=KanataniRyoichi en-aut-sei=Kanatani en-aut-mei=Ryoichi kn-aut-name=金谷良一 kn-aut-sei=金谷 kn-aut-mei=良一 aut-affil-num=1 ORCID= en-aut-name=WuJiri en-aut-sei=Wu en-aut-mei=Jiri kn-aut-name=呉基日 kn-aut-sei=呉 kn-aut-mei=基日 aut-affil-num=2 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Plant type kn-keyword=Plant type en-keyword=Linkage analysis kn-keyword=Linkage analysis en-keyword=Developmental pattern kn-keyword=Developmental pattern END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=3 article-no= start-page=195 end-page=220 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Character Expression and Inheritance of ‘Irregulare’ Spike in Barley kn-title=オオムギにおける不斉条穂の形質発現と遺伝 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=1 ORCID= en-aut-name=SaitoWataru en-aut-sei=Saito en-aut-mei=Wataru kn-aut-name=斉藤渉 kn-aut-sei=斉藤 kn-aut-mei=渉 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=23 end-page=32 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Varietal Variation of Earliness in a Narrow Sense and Its Significance for Adaptation in Barley kn-title=オオムギにおける純粋早晩性の品種変異と適応的意義 en-subtitle= kn-subtitle= en-abstract=本研究ではオオムギの出穂早晩性に関与する内的要因のひとつである純粋早晩性の品種変異とその地理的差異について検討するとともに、播種期と試験地を異にした場合の出穂期に対する内的要因の影響を解析し、純粋早晩性の適応的意義について検討した。春化後高(20℃)24時間日長条件下で栽培したときの止葉展開まで日数を純粋早晩性とする。世界各地のオオムギ1,017品種の純粋早晩性は12日から28日まで変異した。純粋早晩性の変異を地域別にみると、高緯度地域に分布する品種群は晩生であり、低緯度地域の品種群は早生であった。中緯度地域および極低緯度地域(エチオピア)の品種群では純粋早晩性の変異が大きく、この地域には高緯度地域よりも晩生の品種が多く存在した。重回帰分析の結果、倉敷における出穂期の早晩は秋播栽培条件では主として光周反応性によって支配されるが、春播栽培条件では主として純粋早晩性によって支配され、遅播きするほど純粋早晩性の影響が強いことが示された。春播栽培地域である北見においても出穂期は純粋早晩性の影響を強く受けていた。幼穂形成期における日長が十分に長い栽培条件では光周反応性は出穂期の調整要因として作用せず、純粋早晩性が出穂期を支配するものと考えられた。 kn-abstract=Heading time of is a complex character, which is controlled by several internal factors, namely, vernalization requirement, photoperiodic response and earliness in a narrow sense (ENS). The ecological significance of vernalization requirement and photoperiodic response has been clarified. However, the significance of ENS is not explained well. In this study, we examined the varietal variation of ENS, its geographical distribution and significance of ENS for regional adaptability in barley. The ENS was estimated from the number of days from sowing to flag-leaf emergence of sufficiently vernalized plants grown under a 24-h. photoperiod at 20℃ condition. The variation of ENS was determined from the number of leaves and leaf emergence intervals under the controlled condition. The ENS of 1,017 barley varieties ranged from 12 to 28 days. ENS was shortest in varieties from low-latitudes, while it was longer in varieties from high-latitudes and very low-latitudes. Multiple regression analysis for heading time(Y) on the ENS(X1) and the photoperiodic response(X2) was carried out using various data obtained from different experiment sites and sowing times. The multiple correlation was highly significant in all cases. It was clear that the ENS played an important role for determining heading time in the spring-sown conditions, while the photoperiodic response did in the fall-sown condition. en-copyright= kn-copyright= en-aut-name=OkuboKazuo en-aut-sei=Okubo en-aut-mei=Kazuo kn-aut-name=大久保和男 kn-aut-sei=大久保 kn-aut-mei=和男 aut-affil-num=1 ORCID= en-aut-name=YasudaShozo en-aut-sei=Yasuda en-aut-mei=Shozo kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=2 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Heading time kn-keyword=Heading time en-keyword=Geographica distribution kn-keyword=Geographica distribution en-keyword=Adaptability kn-keyword=Adaptability END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=3 article-no= start-page=167 end-page=178 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A linkage study of the two genes conditioning anthocyanin pigmentation in barley kn-title=オオムギのアントシアニン着色に関与する2遺伝子の連鎖研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HayashiJ. en-aut-sei=Hayashi en-aut-mei=J. kn-aut-name=林二郎 kn-aut-sei=林 kn-aut-mei=二郎 aut-affil-num=1 ORCID= en-aut-name=TakahashiR. en-aut-sei=Takahashi en-aut-mei=R. kn-aut-name=高橋隆平 kn-aut-sei=高橋 kn-aut-mei=隆平 aut-affil-num=2 ORCID= en-aut-name=MoriyaI. en-aut-sei=Moriya en-aut-mei=I. kn-aut-name=守屋勇 kn-aut-sei=守屋 kn-aut-mei=勇 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=72 end-page=83 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=On the mildew resistance varieties in barley. kn-title=オオムギのウドンコ病に対する抵抗性品種について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HiuraU. en-aut-sei=Hiura en-aut-mei=U. kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=1 ORCID= en-aut-name=HetaH. en-aut-sei=Heta en-aut-mei=H. kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=3 article-no= start-page=107 end-page=115 dt-received= dt-revised= dt-accepted= dt-pub-year=1961 dt-pub=196105 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Pathogenic variability attributed to hybridization in Erysiphe graminis hordei. Studies on variation in pathogenicity. II. kn-title=オオムギのウドンコ病菌における交雑による病原性の変異 病原性の変異に関する研究II en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HiuraU. en-aut-sei=Hiura en-aut-mei=U. kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=1 ORCID= en-aut-name=HetaH. en-aut-sei=Heta en-aut-mei=H. kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= en-aut-name=TsushimaT. en-aut-sei=Tsushima en-aut-mei=T. kn-aut-name=津島孝宏 kn-aut-sei=津島 kn-aut-mei=孝宏 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=193 end-page=202 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Root Fluorescence Mutants in Barley kn-title=オオムギの根の蛍光変異体に関する研究 en-subtitle= kn-subtitle= en-abstract=オオムギ10,473品種およびコムギ1,140品種を供試して根の蛍光変異体を検索したが、変異体を見出すことはできなかった。オオムギ品種ふじ二条にガンマー線を3世代にわたって90KR照射したM8世代16,480系統の中から根端が紫外線によってピンクに発色する変異体が3系統得られた。これらは同一の突然変異に由来する姉妹系統の可能性が高い。この蛍光変異体は劣性1遺伝子frp(fluorecent reaction pink)に支配される。frpは4H染色体のgl-3近傍に座乗し、その伝達率は正常型の対立遺伝子に比べて0.8程度と低い、frpは致死遺伝子であるがヘテロ維持が可能である。 kn-abstract=Using 10,473 barley accessoins and 16,480 gamma-ray lines, root fluorescence mutants which have been reported in order crops, such as soybean, were screened. No spontaneous mutant was found,but an artificial mutant whose root tips emitted a pink glow under ultraviolet light was obtained by the gamma-ray irradiation. The mutant was controlled by a recessive gene named frp 'fluorescent reaction-pink'. It was poor growth and was lethal. The transmissoin rate of the mutant gene was about 80% of that of the normal allele. Thus the segregation in the heterozygous populations was ca. 4 normal to 1 mutant. The frp gene is located close to gl-3, 'glossy leaf-3' on the 4H chromosome. en-copyright= kn-copyright= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=1 ORCID= en-aut-name=ChangCheng Lin en-aut-sei=Chang en-aut-mei=Cheng Lin kn-aut-name=張成林 kn-aut-sei=張 kn-aut-mei=成林 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Mutation kn-keyword=Mutation en-keyword=Fluorescence kn-keyword=Fluorescence en-keyword=Linkage analysis kn-keyword=Linkage analysis END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=3 article-no= start-page=139 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characters of Barley Resistance to Cereal Aphids (1) Occurrence and abundance of four cereal aphids on barley fields kn-title=オオムギの耐アブラムシ性要因 第1報 圃場における4種アブラムシの発生消長 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=3 ORCID= en-aut-name=FukuokaMariko en-aut-sei=Fukuoka en-aut-mei=Mariko kn-aut-name=福岡まり子 kn-aut-sei=福岡 kn-aut-mei=まり子 aut-affil-num=4 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= affil-num=5 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=3 article-no= start-page=149 end-page=159 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characters of Barley Resistance to Cereal Aphids (2) Nutritional differences with the barley strain kn-title=オオムギの耐アブラムシ性要因 第2報 系統間の栄養成分の差異 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=3 article-no= start-page=161 end-page=170 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characters of Barley Resistance to Cereal Aphids (3) A surveying method for substances responsible for the resistance to aphids by artifical rearing kn-title=オオムギの耐アブラムシ性要因 第3報 人工飼育法による耐性物質の検索 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=2 ORCID= en-aut-name=ShiragaTakashi en-aut-sei=Shiraga en-aut-mei=Takashi kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=55 end-page=62 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Inheritance of Phenol Reaction in the Awn of Barley kn-title=オオムギの芒におけるフェノール着色反応の遺伝性 en-subtitle= kn-subtitle= en-abstract=オオムギの芒ではフェノールを酸化する酵素の活性が強くが、極めて少数の品種では芒のフェノール着色が見られない。本研究では芒のフェノール着色反応の遺伝性を解析した。結果は次の通り。1)芒のフェノール着色反応は優性遺伝子ph(phenol reaction)によって支配されている。2)Phは第2染色体にe-Ph-liの順で配列している。3)芒、穀皮および果実(果皮)の反応が一致しない場合があるが、これが実験誤差か、それぞれの組織で別個の遺伝子が作用していることによるのかは確認を要する。 kn-abstract=The inheritance and geographical distribution of the phenol reaction in rice has been investigated intensively. On the other hand, in the case of barley, almost all of the varieties show positive reaction to phenol, and inheritance study of the trait has not yet been reported. We investigated the phenol reaction of ca. 5,000 barley varieties preserved at the Barley Germplasm Center of Okayama University to find about 30 varieties which show a negative reaction to the phenol. These varieties are mainly from Southwest Asia. The reaction of awn was sharpest at all parts of the plant including seeds. The positive reaction must be the prototype of barley, because Hordeum spontaneum, a possible ancestor of the cultivated barley, shows positive reaction. Inheritance and linkage studies were conducted using varieties with negative reaction and linkage testers with positive reaction. Phenol reaction was dominant and showed a clear 3:1 segregation ratio in the F2 populations, indicating the trait was controlled by a single dominant gene Ph(phenol reaction). Linkage study revealed that Ph was linked with four marker genes(three loci) on the chromosome 2 and independent of 13 other marker genes located on the chromosomes except chromosome 2. Ph may be a useful marker gene and an interesting material for molecular-biological studies. en-copyright= kn-copyright= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=1 ORCID= en-aut-name=ZhangChan Lin en-aut-sei=Zhang en-aut-mei=Chan Lin kn-aut-name=張成林 kn-aut-sei=張 kn-aut-mei=成林 aut-affil-num=2 ORCID= en-aut-name=KanataniRyoichi en-aut-sei=Kanatani en-aut-mei=Ryoichi kn-aut-name=金谷良市 kn-aut-sei=金谷 kn-aut-mei=良市 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Phenol reaction kn-keyword=Phenol reaction en-keyword=Linkage analysis kn-keyword=Linkage analysis END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=63 end-page=68 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Genetic Analysis of Large Trichome in Barley Leaf Blade kn-title=オオムギの葉の剛毛に関する遺伝子分析 en-subtitle= kn-subtitle= en-abstract=岡山大学資源生物科学研究所大麦系統保存施設が保有するオオムギのうち約2,300品種を対象として、葉身の表面に生じる毛(trichome)の変異体を検索した。1)2,300品種のうち9品種(0.4%)が葉身の表面に剛毛を生じた。2)通常の品種では毛茸の鈎状部分の長さが約10μmであるのに対して剛毛型の品種では約40μmであった。Pub遺伝子による長毛はこれよりはるかに長く、剛毛型とは明らかに異なった。3)剛毛型は優性の1遺伝子(Ltr,large trichome)に支配されており、連鎖分析の結果、第7染色体にLtr-s-fsの順に配列されることが明らかになった。 kn-abstract=The inheritance and linkage relationship of a new hairiness trait "large trichome" was investigated in barley. Although the size of large trichome is about four times that of normal one, the character can not be recognized with the naked eye. However, it is easily identified by the roughness of leaf touch. The large trichomes develop on both sides of the leaf blades. The direction of trichome is both acropetal and basipetal. It is clearly distinguished from the extremely long trichome controlled by Pub gene. About 2,300 varieties of our Barley Germplasm Center were screened by the leaf touch to find nine varieties with large trichome. Two of them were six-rowed local variety from Pakistan, and other seven were two-rowed varieties from Europe and Japan. All of them were hulled type. Crosses of six large trichome varieties with a normal Japanese variety resulted in the large trichome type F1s, suggesting the dominant nature of the trait. The large trichome line Hokuiku 17 was crossed with various linkage testres to study the mode of inheritance and the linkage relationship of the gene. In the F2 populations, the large trichome was controlled by a single dominant gene named Ltr (large trichome), which was independentiy inherited from the following marker genes; br and gl-5 on chromosome 1; li and ν on chromosome 2; uz on chromosome 3; K and gl-3 on chromosome 4; trd on chromosome 5; ο on chromosome 6. On the other hand, from the cross between Hokuiku 17 and OUL166, Ltr was found to be linked with s and fs on chromosome 7. Although the allelism test has not been completed, the very low frequency of the large trichome type (9/2,300) indicates that the variant resulted from a recent mutation event, or the fitness of the variant is low in the natural and/or artificial selection. en-copyright= kn-copyright= en-aut-name=SatoKazuhiro en-aut-sei=Sato en-aut-mei=Kazuhiro kn-aut-name=佐藤和広 kn-aut-sei=佐藤 kn-aut-mei=和広 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Trichome kn-keyword=Trichome en-keyword=Linkage analysis kn-keyword=Linkage analysis END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=55 end-page=62 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparison between shoot Regeneration Ability in Calli Derived from Immature Embryos of Wild and Cultivated Barley kn-title=オオムギの近辺野生種と栽培種における未熟胚由来カルスからの再分化能の比較 en-subtitle= kn-subtitle= en-abstract=本研究ではオオムギ近緑野生種Hordeum spontaneum82系統とH.agriocrithon13系統、計2種95系統および世界各地の栽培種87品種を供試して、未熟胚由来カルスからの不定芽再分化能を調査し、近緑野生種と栽培種における再分化能の品種変更およびその地理的分化を比較した。近緑野生種では95系統中94系統がカルスを形成し、そのうち85系統(90.4%)が不定芽を再分化して、不定芽再分化率は系統によって1.2%から75.7%まで幅広く変異した。カルスを形成した94系統における不定芽再分化率の平均値は21.7%、緑色不定芽再分化率は11.5%、アルビノ不定芽再分化率は10.2%であった。H.spontaneumとH.agriocrithonの不定芽再分化率の平均値はそれぞれ21.4%と23.9%で、有意差は認められなかった。しかし、H.spontaneumに含まれる6品種(dawense, ishnatherum, laguncliforme, paradoxon, proskowetzii, spontaneum)の間では不定芽再分化率に差異が認められた。栽培種では供試品種のすべてがカルスを形成し、そのうち73品種(83.7%)が不定芽を再分化して、不定芽再分化率は品種によって3.2%から85.5%まで幅広く変異した。供試品種全体の不定芽再分化率の平均値は25.4%、緑色不定芽再分化率は22.0%、アルビノ不定芽再分化率は3.4%であった。このように、不定芽再分化率に対する緑色不定芽再分化率の占める割合は栽培種で86.6%、近緑野生種で53.0%となり、両者に大きな差が認められた。供試した栽培種を小穂非脱落性に関する遺伝子型によって東亜地域に多く分布するBt bt2型と西域に多く分布するbt Bt2型に分けて不定芽再分化率を比較すると、Bt bt2型の平均値は16.2%、bt Bt2型の平均値は32.3%となり、bt Bt2型の不定芽再分化率が有意に高く、地理的分化が認められた。しかし、近緑野生種では不定芽再分化能の地域間差がみられないので、オオムギの祖先種が栽培化されてから不定芽再分化能の地理的分化が生じたものと考えられる。 kn-abstract=The variation in shoot regeneration obility of calli derived from immature embryos was examined in 95 wild strains, 82 of which were of Hordeum spontaneum and 13 of which were H. agriocrithon, and 87 cultivated varieties collected from various countries or regions of the world. In 85 strains of the wild species, a number of calli regenerated shoots, and their proportion ranged from 1.2% to 75.7%. The average percentage of shoot regenerating calli was 21.7% among the strains that formed calli, 11.5% of which regenerated green and 10.2% albino shoots. On average, 21.4% and 23.9% calli regenerated shoots in H. spontaneum and H. agriocrithon, respectively and there was no significant difference between these values. A significant difference in the percentage of shoot regenerating calli was found among six variants (dawense, ishnatherum, laguncliforme, paradoxon, proskowetzii, spontaneum) which were comprised in H. spontaneum. In 73 varieties of cultivated species, there were shoots regenerating calli likely to wild species, and their proportion ranged from 3.2% to 85.5%. The average percentage of shoot regenerating calli was 25.4%, 22.0% of which regenerated green and 3.4% of which regenerated albino shoots. There was a significant difference in percentage of green shoots regenerating calli against shoots regenerating ones between the wild (53.0%) and cultivated species 886.6%). The two kinds of non-brittle rachis genotypes, Bt bt2 and bt Bt2 are one of the key characters distinguishing the oriental and occidental types of cultivated barley. The average percentages of shoot regenerating calli were 16.2% and 32.3% for the genotypes Bt bt2 and bt Bt2, respectively, suggesting that there is a geographical variation in the shoot regeneration ability of calli in the cultivated species. By contrast, the oriental and occidental strains of wild species showed no difference in the shoot regeneration ability of calli. The geographical variation of shoot regeneration ability differed significantly between wild and cultivated species. This suggests that the geographical variation of shoot regeneration ability occurred after the cultivation of the barley was established. en-copyright= kn-copyright= en-aut-name=RikiishiKazuhide en-aut-sei=Rikiishi en-aut-mei=Kazuhide kn-aut-name=力石和英 kn-aut-sei=力石 kn-aut-mei=和英 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= en-aut-name=YasudaShozo en-aut-sei=Yasuda en-aut-mei=Shozo kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Hordeum spontaneum kn-keyword=Hordeum spontaneum en-keyword=Hordeum agriocrithon kn-keyword=Hordeum agriocrithon en-keyword=Tissue culture kn-keyword=Tissue culture en-keyword=Geographical variation kn-keyword=Geographical variation en-keyword=Shoot regeneration ability kn-keyword=Shoot regeneration ability END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=111 end-page=122 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparison of Resistance to Powdery Mildew between Wild Barley (Hordeum spontaneum C. Koch) kn-title=オオムギの野生種(Hordeum spontaneum C. Koch)と在来種におけるうどんこ病抵抗性の比較 en-subtitle= kn-subtitle= en-abstract=病原菌の変異による抵抗性品種の罹病化は耐病性育種の大きな問題である。永続性のある抵抗性品種を育成するための遺伝資源を得る目的で、病原菌と長期間共進化してきた野生オオムギ(Hordeum spontaneum)と在来種のうどんこ病抵抗性を評価した。材料はH.spontaneum162系統ならびにこれらと産地を同じくする在来品種145系統である。接種には日本のうどんこ病菌H1,h4,h9の他に人為的交雑によって育成した7菌株を加えた10菌株を用いた。第1葉の反応を免疫的抵抗性(i)、高度抵抗性(R)、中度抵抗性(M)および罹病性(S)に分類した。その結果、以下のことが明らかとなった。1)抵抗性(i,R,M)を示す系統の頻度は大部分の菌株において野生種の方が高かった。2)野生種の抵抗性は在来種よりも多数の菌株に対して有効であった。3)クラスター分析の結果、野生種の大部分が同一のグループに属し、在来品種は多くのサブグループに属することが明らかにされた。4)これらの事実から、オオムギの野生種はうどんこ病抵抗性育種の遺伝資源として在来種と同等あるいはそれ以上に有用であるとみられた。 kn-abstract=A total of 162 strains of wild barley, Hordeum spontaneum C. Koch originating from Iran, Iraq, Turkey and Central Asia, were tested for resistance to powdery mildew. Then, the variation of resistance was compared with that of 145 local varieties of cultivated barley (Hordeum vulgare L.) originating from the same region of the wild barley collection. Ten different isolates of the parasite with Japanese origin were separately inoculated onto the first leaves of the host plants. The infection types were classified into the following: i, immunelike; R, highly resistant; M, moderately resistant; and S, highly susceptible. Resistant strains with i, R or M infection type were more frequent among wild barleys as compared with the cultivated forms. It is noteworthy that among these three resistant reactions,the M type was most frequent in the wild barley. To compare the degree of resistance to a total of 10 isolates, the resistance score was calculated in each of the wild and cultivated strains as the following: Scores 1,2,3 and 4 were given to the infection types of i, R, M and S, respectively, and the mean score for 10 isolates was calculated. Wild barley showed significantly low resistance scores as compared with those of cultivated barley. This was also confirmed by the cluster analysis; the cluster with more resistance to 10 isolates consisted of many strains of wild barley. Next, the resistance of wild barley was characterized by their broader effective ranges to different isolates. According to the x2 test for independence of reactions to two different isolates, the resistant factor(s) involved in wild barley was confirmed to be rather non-specific to the parasite. It was concluded that H. spontaneum may be useful genetic resources for the breeding of resistance to powdery mildew as well as local varieties. en-copyright= kn-copyright= en-aut-name=FukuyamaToshinori en-aut-sei=Fukuyama en-aut-mei=Toshinori kn-aut-name=福山利範 kn-aut-sei=福山 kn-aut-mei=利範 aut-affil-num=1 ORCID= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= en-aut-name=SatoKazuhiro en-aut-sei=Sato en-aut-mei=Kazuhiro kn-aut-name=佐藤和広 kn-aut-sei=佐藤 kn-aut-mei=和広 aut-affil-num=3 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Hordeum spontaneum kn-keyword=Hordeum spontaneum en-keyword=Barley kn-keyword=Barley en-keyword=Powdery mildew kn-keyword=Powdery mildew en-keyword=Resistance kn-keyword=Resistance END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=7 end-page=22 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Heterosis in Barley Cultivars kn-title=オオムギの雑種強勢に関する研究 en-subtitle= kn-subtitle= en-abstract=自殖性作物のオオムギでは、小穂脱落をはじめ補足遺伝子によって発現する多くの障害が知られている。このような障害の生じない交雑組合せでF1にどの程度のヘテローシスが現れるか、ヘテローシスの発現と品種分布の地域性との関係があるかどうかについて試験を行った。そのため日本の数品種を共通親とし、世界各地の品種との交雑を行った。結果の大要は以下の如くである。1.六条品種(並性)の交雑では、日本、中国及び朝鮮半島の43品種に、日本の六条皮及び裸麦をそれぞれ交雑し、ヘテローシスの程度を調べた。その結果個体当り収量に最も高いヘテローシスを示したのは朝鮮半島の品種で、F1/MPの交雑平均値1.37、最大のヘテローシスを示した組合せはF1/BPが1.45であった。2.二条品種の場合は、世界各地からの78品種の共通親として日本の2品種を交雑したF1156を調査した。トルコやエチオピアの品種との交雑でヘテローシスが高く、個体当り収量に対するヘテローシスの最大値を示した組合せは、F1/BPがそれぞれ1.66と1.45であった。3.日本特有の半矮性品種の場合は34品種を用い、これらに皮及び裸性の渦性2品種を共通親としたF1を調査した。これらの交雑でもヘテローシスが発現し、皮麦×裸麦のF1でヘテローシスが高かった。4.世界各地の二条品種10用いた片面ダイアレル分析の結果、一般組合せ能力の高い品種(エチオピア)や特定組合せ能力の高い品種(フランス)が見いだされた。5.二条オオムギで収量への寄与が最も大きかった形質は、一株穂数であったが、降雨の多い年次に行った試験結果では1000粒重の影響が大であった。これは、過繁茂による登熟歩合の低下が主因と考えられたが、品種により過繁茂でも登熟歩合がほとんど低下しない組合せがあった。 kn-abstract=In cultivated barley, various hybrid injuries such as brittle rachis and hybrid weakness occur. Therefore, crossing must be done among cultivars to prevent the occurrence of such hybrid injuries. In the present study, we examined the magnitude of the heterosis in the crossings and geographical distribution of barley cultivars concerning heterosis. A few Japanese cultivars were used as the common parent for crossing. (1) In the six-rowed barley, 43 cultivars collected from Japan, China and Korea were crossed with non-uzu covered and naked cultivars, Shiroyo-shigara 2 and Nami-Akashinriki, respectively. The largest magnitude of heterosis in grain yield per plant was obtained in the crossing between the Korean cultivar and one of the Japanese common parents. (2) Heterosis in two-rowed cultivars was investigated using a total of 156 F1S which were cross combinations of cultivars collected from the world with two common male parents, Amagi Nijo and Kawasaigoku. The heterosis was larger larger in the cross combinations with Turkish and Ethiopian cultivars. The largest F1/BP was 1.66 in Turkish and 1.45 in Ethiopian cultivars. (3) As to uzu or semi-dwarf cultivars peculiar to Japan, 34 cultivars were crossed with two common male parents, Sekitori (covered) and Akashinriki (naked). Expression of the heterosis was rather conspicuous in the crosses of covered×naked cultivars than those of covered×covered and naked×naked ones. (4) Half diallel analysis using 10 two-rowed cultivars showed that one Ethiopian cultivar expressed a high grade of general combining ability and that one French cultivar was of higher specific combining ability. (5) The most effective agronomic character of the two-rowed cultivars to the grain yield per piant was number of ears in an ordinary year, while in the wet year it was 1000 grain weight. This was considered to be due to overluxuriant growth accompanied by lowering of the percentage of ripened grain. However, in some cultivars the percentage of ripened grain was not lowered under the overluxuriant growth condition. en-copyright= kn-copyright= en-aut-name=YasudaShozo en-aut-sei=Yasuda en-aut-mei=Shozo kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=1 ORCID= en-aut-name=HayashiJiro en-aut-sei=Hayashi en-aut-mei=Jiro kn-aut-name=林二郎 kn-aut-sei=林 kn-aut-mei=二郎 aut-affil-num=2 ORCID= en-aut-name=MoriyaIsamu en-aut-sei=Moriya en-aut-mei=Isamu kn-aut-name=守屋勇 kn-aut-sei=守屋 kn-aut-mei=勇 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Heterosis kn-keyword=Heterosis en-keyword=Diallel analysis kn-keyword=Diallel analysis en-keyword=Overluxuriant growth kn-keyword=Overluxuriant growth en-keyword=Geographical variety kn-keyword=Geographical variety END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=2 article-no= start-page=49 end-page=54 dt-received= dt-revised= dt-accepted= dt-pub-year=1961 dt-pub=196102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Heterothallism in Erysiphe graminis hordei. Studies on variation in pathogenicity. I. kn-title=オオムギウドンコ病菌のHeterothallism病原性の変異に関する研究 I en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HiuraU. en-aut-sei=Hiura en-aut-mei=U. kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=1 ORCID= en-aut-name=HetaH. en-aut-sei=Heta en-aut-mei=H. kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=2 ORCID= en-aut-name=TsushimaT. en-aut-sei=Tsushima en-aut-mei=T. kn-aut-name=津島孝宏 kn-aut-sei=津島 kn-aut-mei=孝宏 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=33 end-page=42 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparison of Regenerating Ability of Calli Derived from Mature and Immature Embryos in Barley Varieties kn-title=オオムギ品種における完熟胚および未熟胚由来カルスの再分化能の比較 en-subtitle= kn-subtitle= en-abstract=本研究では世界各地のオオムギ132品種を供試して完熟胚と未熟胚からのカルス誘導と再分化能について調査し、材料植物が有する遺伝要素と、外植片の違いが再分化能のどのように関与しているかを考察した。供試品種のほとんどがカルスを形成したが、全くカルスを形成しない品種もあった。エチオピアの品種にはカルス形成の悪いものが多くみられた。完熟胚培養系ならびに未熟胚培養系ともに供試品種によってカルスの増殖量に幅広い変異があり、カルスがほとんど形成されない品種から、著しく増殖する品種まであった。カルスの形態的特徴に関しては、完熟胚培養系ではほとんどの品種が水っぽいカルスを形成したのに対して、未熟胚培養系では品種によって、水っぽいタイプ、硬くしまったタイプ、もろく崩れやすいタイプの3タイプを形成し、両培養系間に大きな差異が認められた。完熟胚培養系の場合、再分化するのはほとんどが不定根であり、不定芽を再分化したのは日本の関東二条5号、ふじ二条および紫麦の3品種だけであった。一方、未熟胚培養系では、21.2%の品種が不定芽を再分化した。不定芽を再分化した品種は朝鮮半島や日本に多く、反対にエチオピアや西南アジアの品種には再分化するものが少なかった。供試品種における不定根の再分化率と不定芽の再分化率の間には相関関係はなかった。(r=0.05)。両培養系の間ではカルス増殖量について相関関係はなく(r=0.07)、不定根の再分化率については低い値ではあるが有意な正の相関があった(r=0.24)。また、未熟胚培養系におけるカルス増殖量と不定芽の再分化率についても相関関係は認められなかった(r=0.14)。二条品種と六条品種の間では、完熟胚培養系の不定根再分化率を除いて差は認められなかった。 kn-abstract=The callus forming ability and regenerating ability of the calli derived from mature and immature embryos of 132 barley varieties were examined. These materials were taken from a world-wide collection preserved at the Barley Germplasm Center of Okayama University. The callus forming ability varied widely according to genotype in both mature and immature embryos, but the varieties collected from Ethiopia showed low callus forming ability. Calli derived from mature embryos generally did not regenerate shoots, except for three Japanese varieties. The frequency of shoot regeneration from the calli derived from immature embryos was somewhat higher than that from those derived from mature embryos. Many of the Korean and Japanese varieties had a high shoot regenerating ability. However, few of the varieties from Ethiopia and Southwest Asia had a high shoot regenerating ability. No correlation was observed btween root regenerating ability and shoot regenerating ability of the varieties. No correlation was observed between callus proliferation and root regenerating ability between calli derived from mature and immature embryos. We could not find any difference in the shoot regenerating ability btween the two-rowed and six-rowed genotypes. en-copyright= kn-copyright= en-aut-name=RikiishiKazuhide en-aut-sei=Rikiishi en-aut-mei=Kazuhide kn-aut-name=力石和英 kn-aut-sei=力石 kn-aut-mei=和英 aut-affil-num=1 ORCID= en-aut-name=YasudaShozo en-aut-sei=Yasuda en-aut-mei=Shozo kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Tissue culture kn-keyword=Tissue culture en-keyword=Mature embryo kn-keyword=Mature embryo en-keyword=Immature embryo kn-keyword=Immature embryo en-keyword=Regenerating kn-keyword=Regenerating END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=4 article-no= start-page=173 end-page=183 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=197303 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=オオムギ品種における穂揃い性の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=沖永康男 kn-aut-sei=沖永 kn-aut-mei=康男 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=71 end-page=81 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Varietal Variation and Effects of Some Major Genes on Salt Tolerance in Barley Seedlins kn-title=オオムギ幼植物の耐塩性における品種変異と各種主働遺伝子の効果 en-subtitle= kn-subtitle= en-abstract=本研究では耐塩性品種を見出す目的で、世界各地のオオムギ5,182品種について幼植物の耐塩性の品種変異ならびにその地理的変異、および主要形質との関係を調査した。幼植物の耐塩性を地域別に比較すると、朝鮮半島および日本の品種が強く、ネパールと西南アジアの品種が弱い傾向にあった。次に、条性や皮裸性など、オオムギにおける主要形質と幼植物の耐塩性との関係を調べるために、5,182品種を主要形質に分けて耐塩性を比較した結果、六条品種は二条品種より、皮品種は裸品種より、小穂非脱落性のE型はW型より、日本と朝鮮半島に分布する六条の渦品種は、同地域の六条の並品種よりもそれぞれ耐塩性が有意に強かった。さらに、供試品種を条性、皮裸性および小穂非脱落性の3形質の組合せで8タイプに分類して幼植物耐塩性を比較したところ、皮裸性、小穂非脱落性が同じ場合、二条品種と六条品種の間では有意差がなく、また、皮裸性と小穂非脱落性の組合せによって耐塩性の強弱が逆転した。同質遺伝子系統を用いて幼植物の耐塩性を比較したところ、品種群でみられた条性、皮裸性の間における耐塩性の差異は、品種群の遺伝的背景の差異によるものとみられた、一方、並渦性でみられた耐塩性の差異は主働遺伝子の副次的効果によるものとみられた。 kn-abstract=In order to select the salt tolerant cultivars, and to evaluate the effects of some major genes on salt tolerance in barley, 5,182 varieties and 368 isogenic lines were tested by exposing to 1.0 and 0.5M sodium chloride at the seedling stage. The average rating of salt tolerance was higher in varieties from Korea and Japan , but lower in varieties from Nepal and Southwest Asia. When the varieties were grouped by several morphological characters, six-rowed, covered, E type of nonbrittle rachis and uzu type groups were more tolerant than two-rowed, naked, W type and non-uzu type counterparts, respectively. A comparison between the isogenic pairs revealed that the uzu type was more tolerant than non-uzu type, but other major gene pairs did not show any obvious differences. These findings indicate that the uz gene affects the salt tolerance, and diferences between varietal groups characterized by other major genes are due to the difference in the genetic background among these varieties. en-copyright= kn-copyright= en-aut-name=ManoYoshiro en-aut-sei=Mano en-aut-mei=Yoshiro kn-aut-name=間野吉郎 kn-aut-sei=間野 kn-aut-mei=吉郎 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Seedling kn-keyword=Seedling en-keyword=Salt tolerance kn-keyword=Salt tolerance en-keyword=Screening kn-keyword=Screening en-keyword=Isogenic line kn-keyword=Isogenic line END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=1 article-no= start-page=43 end-page=53 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Genetical Studies on Callus Growth and Plant Regeneration from Immature Embryos in Barley kn-title=オオムギ未熟胚培養系におけるカルス生長量及び植物体再分化能の遺伝解析 en-subtitle= kn-subtitle= en-abstract=オオムギ培養細胞の再分化の制御や細胞選抜の実用化に寄与するために、植物体再分化能の遺伝様式を解析した。未熟胚培養系においては世界各地の99品種についてカルス生長量及び植物体再分化能の調査を行ったところ、両形質共に連続的な変異を示し、複数の遺伝子あるいは微動遺伝子が関与していることが推察された。カルス生長量及び植物体再分化能の遺伝様式を調べるために、6品種で正逆総当たり交雑を行い、ダイアレル分析を行った。カルス生長量については、非対立遺伝子間の相互作用(エピスタシス)があると推察された。一方、植物体再分化能については、特定組み合わせ能力が高かったP1(J232)を除いた副ダイアレルの分析によって、植物体再分化能の遺伝変異は主として遺伝子の相加効果によるものと推察され、その平均優性度は0.42であった。遺伝率の推定値は狭義で0.78、広義で0.86といずれも高かった。 kn-abstract=Immature embryos of 99 varieties of barley were cultured to investigate the ability of callus growth and plant regeneration. These two in vitro traits showed wide and continuous variations among the barley varieties tested. Ability of callus growth, which were evaluated by callus diameter ranged from 3.9mm to 11.2mm, and ability of plant regeneration from the calli ranged from 0% to 100%. A set of complete diallel crosses was made using six cultivars as the parents which differed in ability of callus growth and plant regeneration. The Vr/Wr graphical analysis showed that there were epistasis, or interaction among nonallelic genes for callus growth. As to ability of plant regeneration, no epistasis existed in the subdiallel without P1 (J232) which showed high specific combining ability, and it was controlled by a simple additive dominance genetic system. The mean degree of dominance(0.42) was relatively low and the broad(0.86) and narrow(0.78) sense heritabilities were high. en-copyright= kn-copyright= en-aut-name=ManoYoshiro en-aut-sei=Mano en-aut-mei=Yoshiro kn-aut-name=間野吉郎 kn-aut-sei=間野 kn-aut-mei=吉郎 aut-affil-num=1 ORCID= en-aut-name=RikiishiKazuhide en-aut-sei=Rikiishi en-aut-mei=Kazuhide kn-aut-name=力石和英 kn-aut-sei=力石 kn-aut-mei=和英 aut-affil-num=2 ORCID= en-aut-name=YasudaShozo en-aut-sei=Yasuda en-aut-mei=Shozo kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Tissue culture kn-keyword=Tissue culture en-keyword=Plant regeneration kn-keyword=Plant regeneration en-keyword=Diallel analysis kn-keyword=Diallel analysis END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue=1 article-no= start-page=21 end-page=28 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=1999 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=QTL Mapping for Water Sensitivity in Barley Seeds kn-title=オオムギ種子感水性のQTLマッピング en-subtitle= kn-subtitle= en-abstract=Steptoe×Morex(S/M)およびHarrington×TR306(H/T)の倍加半数体(DH)系統を用いて種子の感水性のQTL解析を行った。DH系統は連続的な超越分離を示し、S/Mでは3個、H/Tでは1個の有意なQTLが見出され、それぞれ感水性の変異の約1/4が説明された。これらのQTLの近傍には発芽速度などの発芽に関する他のQTLが見出された。 kn-abstract=Sensitivity to water condition during germination is an important character in malting barley because it affects malt poduction. Water sensitivity in malting barley is defined as the defference in germination percentage between the seeds in standard and excess-water conditions. Usually standard and excess-water condition means 4 and 8 ml water in a 90 mm perti dish, respectively. Takeda and Fukuyama (1983) examined more than 4,000 barley accessions and found 0〜100% water sensitivity. This means complete to no suppression of germination by an excess-water condition. In this study, quantiative trait loci (QTL) analysis was applied to analize the inheritance of water sensitivity. Two sets of doubled haploid (DH) populations derived from Steptoe×Morex (S/M) and Harrington×TR306 (H/T) crosses,were grown in the experiment field of the Research Institute for Bioresources, Okayama University. Bulbosum method was adapted to develop DH lines (Chen and Hayes 1989). Water sensitivities of 150 (S/M) and (H/T) lines and their parents were evaluated using 50 seeds each and the germination test (25℃, 4 days) was repeated four times. Water sensitivity showed a large variation (Fig.1) and the heritability of the trait was 0.78 and 0.72 in S/M and H/T, respectively, indicating that about three-quarters of the phenotypic variance was genetic in origin. Three (S/M) and one (H/T) significant QTL were detected and 23% (S/M) and 25% (H/T) of the phenotypic variance was explained by the QTL. These QTL were located on chromosomes 2H,3H,6H and 7H (Table 1, Fig. 2). QTL relating to germination traits were lacated near the QTL controlling water sensitivity (Fig. 3). Interaction among the QTL was small (Table 2). Marker-assisted selection was effective (Table 3). en-copyright= kn-copyright= en-aut-name=IwasaTomohiko en-aut-sei=Iwasa en-aut-mei=Tomohiko kn-aut-name=岩佐友彦 kn-aut-sei=岩佐 kn-aut-mei=友彦 aut-affil-num=1 ORCID= en-aut-name=TakahashiHidekazu en-aut-sei=Takahashi en-aut-mei=Hidekazu kn-aut-name=高橋秀和 kn-aut-sei=高橋 kn-aut-mei=秀和 aut-affil-num=2 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=barley kn-keyword=barley en-keyword=water sensitivity kn-keyword=water sensitivity en-keyword=QTL analysis kn-keyword=QTL analysis END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=2 article-no= start-page=139 end-page=149 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199003 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Mechanical Transmission of Barley Yellow Mosaic Virus and Diagnosis of the Virus kn-title=オオムギ縞萎縮病の汁液接種検定並びに診断 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=MitsuhataKōji en-aut-sei=Mitsuhata en-aut-mei=Kōji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=9 end-page=23 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Secretion of Defensive Substance by Carabidae and Brachinidae kn-title=オサムシ科とクビボソゴミムシ科の防御物質の分泌 en-subtitle= kn-subtitle= en-abstract=オサムシ科とクビボソゴミムシ科の防御物質の分泌について系統分類学の視点から250種以上を調べた。属レベル共通性が見られたが、中には亜属や種での特異性のある成分が検出された。防御物質の分泌に関する器官は左右一対の多数の小型の生合成器官、大きな貯蔵?、それをつなぐ輸送管と末端環節間膜に開孔する分泌管より構成されていた。体の大小、管の嵌入場所や貯蔵?の形などで、種による特徴があったが、生合成器官の形に相応して生成物質に特徴があった。すなわち、球形器官は短鎖有機酸を、紐状器官はメタクレゾールを、板状器官はベンゾキノンを生成した。有紀酸の分泌種としてはギ酸分泌種があり、この類にあっては副成分として2トリデカノンを含む類とエステルを含む類があった。オサムシ族、ナガゴミムシ族その他かなり多くの種類は分岐アミノ酸であるバリン、イソロイシンとロイシンやリジンから生成されるメタアクリル酸、チグリン酸、エチルアクリル酸、セネシオ酸やクロトン酸と各々の飽和酸の幾つかを構成成分としていた。一般的に属レベルで同じ成分が多かったが、例外的に種の特異性成分もあった。メタクレゾールはキベリアオゴミムシ亜属を例外とするアオゴミムシ類とヨツボシゴミムシ類で検出された。ベンゾキノン類はキベリアオゴミムシ亜属が分泌し、亜属で特徴を示した。この類は貯蔵?のみからの体温での分泌であった。クビボソゴミムシ科もキベリアオゴミムシ類と同じ組成のベンゾキノンを分泌するが、貯蔵?に接して茶褐色をした酸化反応室を有し約100℃の高温噴射を爆発音と共に行った。 kn-abstract=The secretion of defensive substances by more than 250 species of carabid and brachinid beetles was investigated, and discussed from a phylogenetical viewpoint. The morphological of secretion glands and secreted components were identified in the genus level. Specific subgenus and species had peculiar characteristics. The pair of defensive organs consisted of many small synthetic lobes, large reservoirs, collecting ducts from lobes to reservoirs and secretion ducts from reservoirs. The pair dust opened at the last sternal intersegmental membrane. There was species specificity in the reservoir shape, synthetic lobe shape and entering site of the collecting ducts into reservoirs. The spherical shape lobes produced short chain fatty acids, the elongated shape lobes produced m-cresol and the thick board shape lobes produced benzoquinones. Two types of fatty acid-secreting beetles were found, one secreted formic acid, and the other mixed short chain fatty acids. These short chain fatty acids were metabolized from some amino acids, methacrylic acid was from valine; tiglic acid, α-ethyacrylic acid and angelic acid were from isoleucine; senecioic acid was from leucine and crotonic acid was from lysine. These unasaturated acids were sometimes detected with a trace amount of corresponding saturated acids. A few species secreted larger amounts of saturated acids with a trace amounts of unsaturated acids. Fatty acid composition was generally genus-specific. In a few cases, however, species-specific acid composition was also observed. m-Cresol-secreting beetles were observed only in Panagaeni and Chlaeniini except subgenus Chlaenius Chlaeniini except subgenus Chlaenius Chlaeniellus beetle. 1,4-Benzoquinone and 2-methyl-1,4-benzoquinone-secreting beetles were observed in subgenus C. Chlaeniellus. This beetle secreted these substances at body temperature from a reservoir with no sound. Brachinidae beetles, called bombardier beetles, have a pair of brownish reaction chamber connected with a reservoir. They sprayed the two benzoquinones at about 100℃ making sound. en-copyright= kn-copyright= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=Defensive secreting substance kn-keyword=Defensive secreting substance en-keyword=Synthetic organ kn-keyword=Synthetic organ en-keyword=Carabidae kn-keyword=Carabidae en-keyword=Brachinidae kn-keyword=Brachinidae en-keyword=Phylogenetic relation kn-keyword=Phylogenetic relation END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=93 end-page=100 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparative study of the pygidial defensive systems of adults of Carabidae (Coleoptera) kn-title=オサムシ類成虫の尾部防御分泌系の比較 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=YamashitaT. en-aut-sei=Yamashita en-aut-mei=T. kn-aut-name=山下俊和 kn-aut-sei=山下 kn-aut-mei=俊和 aut-affil-num=2 ORCID= en-aut-name=ShiragaT. en-aut-sei=Shiraga en-aut-mei=T. kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=1 article-no= start-page=47 end-page=54 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680329 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Oviposition and egg adhesive substances of tent caterpillar, Malacosoma neustria L.(Lepidopiera: Lasiocampidae). kn-title=オビカレハMalacosoma neustria L. (Lepidoptera: Lasiocampidae)の産卵習性と卵固着物質 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=SugiyamaS. en-aut-sei=Sugiyama en-aut-mei=S. kn-aut-name=杉山章平 kn-aut-sei=杉山 kn-aut-mei=章平 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=67 end-page=71 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Oviposition Factors of Ponticulothrips diospyrosi on Persimmon Seedling Leaves kn-title=カキクダアザミウマの産卵要因の解析 en-subtitle= kn-subtitle= en-abstract=カキ芽出し苗を用いてカキクダアザミウマ(Ponticulothrips diospyrosi Haga et Okajima)の産卵要因を解析した。羽化した新成虫は、高温及び低温条件を経なくても、本葉が展開する以前の苗があればゴールを形成し産卵した。温度に関しては、15℃、20℃でゴールを形成したが、15℃では産卵しなかった。しかし、15℃でゴールを形成した後、25℃に移すと産卵した。25℃ではゴールは形成されず、従って産卵もしなかった。日長は産卵に影響しなかった。本葉の展開した苗に雌成虫を放飼してもゴールは形成されず。産卵もしなかったが、展開直後の本葉を直径3.2mmのビーバーラインチューブに巻入れると産卵した。一方、老熟した本葉を巻入れても産卵しなかった。さらに、葉に食害痕のみられなかった場合にも産卵しなかった。以上の結果、野外で年一化の生活史をする本アザミウマの雌成虫の産卵には、高温長日及び低温短日を経る必要がなく、20℃〜25℃で巻いた状態のカキの若葉が存在すれば産卵可能であると推定される。 kn-abstract=The thrips Ponticulothrips diospyrosi Haga et Okajima is an univoltine species which aestivates and overwinters in the adult form in the fields. The oviposition factors of the thrips were investigated on persimmon seedling leaves. The adult female could make a gall and oviposit inside it in vitro. Female could make a gall and oviposit at 20℃, but only make a gall at 15℃. Although it could neither make a gall nor oviposit at 25℃, it oviposited at than temperature when placed on a gall. Female neither made a gall nor oviposited on an extended young leat. However, it could oviposit on the young leaf rolled artificially in a line tube (3.2 mm diam.), but not on the old one. These findings show that univoltine of the thrips may be regulated by the phenology of the host plant and females can oviposit whenever they are placed on the seedlings at the optimum oviposition temperature. en-copyright= kn-copyright= en-aut-name=UchiyamaKeiji en-aut-sei=Uchiyama en-aut-mei=Keiji kn-aut-name=内山圭二 kn-aut-sei=内山 kn-aut-mei=圭二 aut-affil-num=1 ORCID= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=3 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Oviposition factor kn-keyword=Oviposition factor en-keyword=Thrips kn-keyword=Thrips en-keyword=Ponticulothrips diospyrosi kn-keyword=Ponticulothrips diospyrosi en-keyword=Gall kn-keyword=Gall en-keyword=Persimmon kn-keyword=Persimmon END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=3 article-no= start-page=141 end-page=148 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Variations of calcium, phosphorus, magnesium, and cadmium contents in anomalous-vertebrae fish induced by cadmium kn-title=カドミウムによって誘発された脊椎骨異常魚のカルシウム, 燐, マグネシウムおよびカドミウム含有量の変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue=1 article-no= start-page=13 end-page=18 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the odour components of stink and squash bugs kn-title=カメムシ類の悪臭成分に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamashitaT. en-aut-sei=Yamashita en-aut-mei=T. kn-aut-name=山下俊和 kn-aut-sei=山下 kn-aut-mei=俊和 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=2 article-no= start-page=55 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680624 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the viruses of plants in Compositae in Japan I. Tomato aspermy virus from chrysanthemum kn-title=キク科植物のウイルスに関する研究 I.キクから分離されるtomato aspermy virus en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name=AsataniM. en-aut-sei=Asatani en-aut-mei=M. kn-aut-name=麻谷正義 kn-aut-sei=麻谷 kn-aut-mei=正義 aut-affil-num=2 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=175 end-page=185 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Close Relationship between Serotype and Pathogenicity of Cucumber Mosaic Virus kn-title=キュウリモザイクウイルスの血清型と病原性との関連性 en-subtitle= kn-subtitle= en-abstract=日本で分離されたキュウリモザイクウイルス(CMV)のY血清型あるいはP血清型に属する68分離株をタバコに汁液接種した結果、それらは病徴から4つのタイプに類別することができた。それらのうち、ユリからの14分離株はタバコには全身感染しなかった。タバコに全身感染する分離株のうち、Y血清型のすべての分離株は接種後長期間経過しても、新葉にモザイク、えそ、奇形などの病徴が常に認められたが、P型では接種後の日数が経過すると新しく展開した葉には病徴が認められないことが多かった。また、CMVのそれぞれの血清型の分離株を各種植物に接種し、宿主範囲および病徴を調べた結果、ユリからの分離株はタバコ、トマトなどのナス科植物やキュウリなどに全身感染しない点で、これまでに報告されている多くの系統とは異なっていた。また、ユリ以外の植物から得られた分離株では、数種のナス科植物、エンドウ、アズキ、トウモロコシなどにおいて、血清型と病原性との間に明確な関連が認められた。 kn-abstract=Sixty-eight isolates of cucumber mosaic virus (CMV) belonging to the Y serotype (serogroup) or P serotype (serotype U) in Japan were inoculated to Nicotiana tabacum cv. White Burley and the symptoms were observed up to three months after inoculation. These isolates were divided into four goups accoding to symptomatology on tabacco plant. None of the isoletes obtained from lilies infected tobacco plants systemically. The plants inoculated with isolates of the P serotype showed systemic mosaic, but only occasional mild symptoms were observed on newly developed leaves. On the other hand, all isolates of Y serotype showed symptoms consisting of mosaic, distortion and/or necrosis throughout the experimental period. In another experiment, reactions of some selected test plants to 30 isolates belonging to the Y or P serotype were examined. Lily isolates may be unique strain of CMV as they did not cause systemic infections on Lycopersicon esculentum, Solanum melongena or Cucumis sativus. Host range tests using 16 isolates originally obtained from plants other than lilies demonstrated a close relationship between serotype and pathogenicity to some test plants including N.tabacum, N. clevelandii, L.esculentum, Pisum sativum, phaseolus angularis and Zea mays. en-copyright= kn-copyright= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=1 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Cucumber mosaic virus kn-keyword=Cucumber mosaic virus en-keyword=Serotype kn-keyword=Serotype en-keyword=Pathogenicity kn-keyword=Pathogenicity END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=4 article-no= start-page=175 end-page=186 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on cumber green mottle mosaic virus in Japan. I. Identification of the virus. kn-title=キュウリ縁斑モザイクウイルスに関する研究 第1報 病原ウイルスの同定 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= en-aut-name=AsataniM. en-aut-sei=Asatani en-aut-mei=M. kn-aut-name=麻谷正義 kn-aut-sei=麻谷 kn-aut-mei=正義 aut-affil-num=3 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=4 article-no= start-page=187 end-page=197 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on cumber green mottle mosaic virus in Japan. II. Some experiments on virus transmission. kn-title=キュウリ縁斑モザイクウイルスに関する研究 第2報 伝搬に関する2,3の実験 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= en-aut-name=AsataniM. en-aut-sei=Asatani en-aut-mei=M. kn-aut-name=麻谷正義 kn-aut-sei=麻谷 kn-aut-mei=正義 aut-affil-num=3 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=4 article-no= start-page=199 end-page=207 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on cumber green mottle mosaic virus in Japan. III. Inactivation of CGMMV in infected plant tissue buried in soil by methyl bromide. kn-title=キュウリ縁斑モザイクウイルスに関する研究 第3報 メチルブロマイドによる土中のウイルスの不活化 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= en-aut-name=AsataniM. en-aut-sei=Asatani en-aut-mei=M. kn-aut-name=麻谷正義 kn-aut-sei=麻谷 kn-aut-mei=正義 aut-affil-num=3 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=3 article-no= start-page=225 end-page=240 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Host Range and Properties of Bean Yellow Mosaic Virus (BYMV) Infecting Crocus, and Serological Relationships among Three Strains of BYMV kn-title=クロッカスから分離されたbean yellow mosaic virus (BYMV)の諸性質並びにBYMV 3系統の血清学的類縁関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KaneshigeHiroshi en-aut-sei=Kaneshige en-aut-mei=Hiroshi kn-aut-name=兼重寛 kn-aut-sei=兼重 kn-aut-mei=寛 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=105 end-page=116 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of Light Irradiation on the Absorption of Rubidium in Chlorella kn-title=クロレラのルビジウム吸収に対する光の影響 en-subtitle= kn-subtitle= en-abstract=クロレラのルビジウム吸収に対する光照射の影響について検討した。その結果、光照射によってルビジウム吸収が明らかに促進されることが認められた。各種代謝阻害剤による実験の結果から、明条件でのクロレラによるルビジウム吸収と酸素発生の両者に対して、DCMUの添加が最もよく一致した影響を示し、ルビジウム吸収の光促進効果と光合成過程との密接な関連が示唆された。 kn-abstract=The effect of light irradiation on the absorption of rubidium was examined in Chlorella. Rubidium absorption in Chlorella was clearly stimulated by the irradiation of light. To clarify the mechanism of light-stimulation on rubidium absorption, experiments were carried out using several metabolic inhibitors; DCMU, NaCN,DNP,CCCP and ouabain. Among the metabolic inhibitors used, DCMU had the most similar effects on the rubidium absorption and oxygen evolution under light condition. These findings suggest a close correlation between the light-stimulation on rubidium absorption and the photosynthetic process in Chlorella. en-copyright= kn-copyright= en-aut-name=HachiyaKinji en-aut-sei=Hachiya en-aut-mei=Kinji kn-aut-name=蜂谷欽司 kn-aut-sei=蜂谷 kn-aut-mei=欽司 aut-affil-num=1 ORCID= en-aut-name=MoritsuguMasumi en-aut-sei=Moritsugu en-aut-mei=Masumi kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=2 ORCID= en-aut-name=KawasakiToshio en-aut-sei=Kawasaki en-aut-mei=Toshio kn-aut-name=河ア利夫 kn-aut-sei=河ア kn-aut-mei=利夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Chlorella kn-keyword=Chlorella en-keyword=Light-stimulation kn-keyword=Light-stimulation en-keyword=Metabolic inhibitors kn-keyword=Metabolic inhibitors en-keyword=Oxygen evolution kn-keyword=Oxygen evolution en-keyword=Rubidium absorption kn-keyword=Rubidium absorption END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=25 end-page=33 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=クロレラの培養ならびに無機養分要求性について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=河崎利夫 kn-aut-sei=河崎 kn-aut-mei=利夫 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=堀士郎 kn-aut-sei=堀 kn-aut-mei=士郎 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=鬼頭俊而 kn-aut-sei=鬼頭 kn-aut-mei=俊而 aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=107 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Factors on the termination of adult diapause and reproductive development in Gastrophysa atrocyanea Mot. (Coleoptera: Chrysomelidae) kn-title=コガタルリハムシの休眠覚醒と生殖器官の発育に関する要因 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AshidaY. en-aut-sei=Ashida en-aut-mei=Y. kn-aut-name=芦田栄徳 kn-aut-sei=芦田 kn-aut-mei=栄徳 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=63 end-page=72 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Habitat segregation of the Sitophilus Complex (Coleoptera: Curculionidae), with special reference to the reduction of body size kn-title=コクゾウ類Sitophilus Complexの小型化と棲み分けについて en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ObaraK. en-aut-sei=Obara en-aut-mei=K. kn-aut-name=尾原和夫 kn-aut-sei=尾原 kn-aut-mei=和夫 aut-affil-num=1 ORCID= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=2 ORCID= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=5 end-page=14 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=コクヌストモドキ, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae)のキノン類分泌とそれが他種昆虫におよぼす毒性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page=1 end-page=15 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=197303 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A new virus of wheat. barley and several other plants in Gramineae. wheat yellow leaf virus kn-title=コムギ,オオムギなどに発生する新ウイルス,コムギ黄葉ウイルス en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=2 ORCID= en-aut-name=HetaH. en-aut-sei=Heta en-aut-mei=H. kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=3 ORCID= en-aut-name=HiuraU. en-aut-sei=Hiura en-aut-mei=U. kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=229 end-page=237 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Production of Strain of Erysiphe graminis f. sp. tritici That Are Pathogenic on Barley and Agropyron kn-title=コムギうどんこ病菌株間の種内交雑におけるオオムギおよびカモジグサに対する病原性菌系の作出 en-subtitle= kn-subtitle= en-abstract=コムギうどんこ病菌株T3およびt4は本来オオムギおよびカモジグサに対して非病原性である。(Table 1)。その菌株T3とt4を交雑し、オオムギおよびカモジグサに対して交雑親菌系より強い病原性を持った菌糸を選抜して交雑を行った。その交雑菌系からさらに交雑菌系よりも強い病原性をもった菌系を選抜することを4回繰り返し、各世代における雑種菌系の本来の宿主ではないオオムギ品種およびカモジグサ系統に対する病原性の変化を調べ、宿主特異性について解析を試みた。コムギうどんこ病菌株間の交雑の繰り返しと交雑親より強い病原性を現わした菌系の選抜により得られた各世代の雑種菌系を、オオムギ品種鹿児島裸およびロシヤ9号に接種した結果、徐々に病原性が強くなる菌系が増加してきた。(Table 2および3)。同様にコムギうどんこ病菌株間の交雑と選抜により得られた雑種菌系を、カモジグサ系統のAgropyron E.e.およびAgropyron Y.3.に接種した結果、各世代における菌系の中に明らかに病原性をもつ菌系が作出された。(Table 4 および5)。このように非病原性菌系の交雑により病原性菌系が作出されることは、多数の微働病原性遺伝子の累積的効果によって生じたもので、この微働病原性遺伝子はformae speciales の分化にも深い関係をもっていると考えられた。イネ科植物うどんこ病菌が、本来宿主ではない他属の植物に非特異的病原性を示したことは、著者の一人部田が先に報告したFlorの遺伝子対遺伝子説のような相互作用に基づくものではないという考えを指示しているものと言える。 kn-abstract=Two isolates of Erysiphe graminis f. sp. tritici, T3 and T4, were crossed and selected for pathogenicity. The corssing and selection process were repeated five times with the progenies. Isolates of each generation were inoculated on the non-hosts, Hordeum vulgare and Agropyron tsukushiense var. transiens OHWI, and parasitism and pathogenicity were investigated with respect to host specificity.As shown in Tables 2 to 5, the process of crossing and selection produced isolates which were pathogenic on barley cultivars, 'Kagoshimahadaka' or 'Russian 9' or Agropyron tsukushiense ecotypes 'Agropyron Early ecotype' or 'Agropyron Yezoense 3'.Factors affecting the degree of pathogenicity appear to be under polygenic control. The cumulative effect of minor genes may play a role in the differentiation of formae speciales suggesting a new concept of pathogenicity in the powdery mildew of grasses. en-copyright= kn-copyright= en-aut-name=HetaHideo en-aut-sei=Heta en-aut-mei=Hideo kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=1 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Wheat powdery mildew kn-keyword=Wheat powdery mildew en-keyword=Host specific kn-keyword=Host specific en-keyword=Pathogenicity kn-keyword=Pathogenicity en-keyword=Properties kn-keyword=Properties en-keyword=Barley kn-keyword=Barley en-keyword=Agropyron kn-keyword=Agropyron END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=61 end-page=68 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Phenol Reaction of Kernels and Chromosome Location of Phenol Reaction Genes in the Genus Triticum kn-title=コムギ属植物における穀粒のフェノール反応の変異およびフェノール反応に関与する遺伝子の座乗染色体 en-subtitle= kn-subtitle= en-abstract=1. コムギ属における穀粒のフェノールによる着色反応を調査した結果、二粒系コムギでは着色する系統としない系統が存在するが、普通系コムギでは3,200以上の品種を供試したにもかかわらず、穀粒がフェノールによって着色しない系統は見いだされなかった。合成コムギ(AABBDD)にはフェノールによって着色しない系統が見出された。2. 異数体分析によって普通系コムギの穀粒のフェノール反応を支配する遺伝子が2Aと2D染色体に座乗することが明らかになり、2B染色体にも穀粒のフェノール反応を支配する遺伝子の存在が認められ、コムギの第2同祖群の全ての染色体にフェノール反応遺伝子が存在することが確かめられた。3. フェノール反応遺伝子と無葉耳遺伝子が見出されているイネ科植物の染色体の同祖性について考察した。 kn-abstract=A total of 3,606 accessions of the genus Triticum involving diploid, tetraploid, hexaploid, and synthetic wheat and 7 Aegilops materials were tested for the phenol reaction in kernels. All hexaploid wheat showed a positive reaction to phenol, but deffered in staining degree. One of the synthetic wheat lines showed a negative reaction to phenol. Using monosomics, ditelosomics and nulli-tetrasomics in the common wheat cv Chinese Spring (Triticum aestivum L.), and Langdon (Triticum turgidum var. durum) disomic substitutions, genes controlling phenol reaction of kernels were located on chromosomes 2A, 2B and 2D. Synteny of the chromosome region involving the phenol reaction gene in some gramineous plants was discussed. en-copyright= kn-copyright= en-aut-name=ChangCheng Lin en-aut-sei=Chang en-aut-mei=Cheng Lin kn-aut-name=張成林 kn-aut-sei=張 kn-aut-mei=成林 aut-affil-num=1 ORCID= en-aut-name=TakedaShin en-aut-sei=Takeda en-aut-mei=Shin kn-aut-name=武田真 kn-aut-sei=武田 kn-aut-mei=真 aut-affil-num=2 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Phenol reaction kn-keyword=Phenol reaction en-keyword=Triticum kn-keyword=Triticum en-keyword=Chromosome kn-keyword=Chromosome END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=61 end-page=68 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=コムギ縞萎縮病の病原ウイルス en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=159 end-page=166 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=196501 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the mechanism of primary infection of wheat scab in relation to the environmental condition. IV. Effect of humidity and water on ascospore dischage. kn-title=コムギ赤カビ病の第一次発生の伝染機構並びに環境条件に関する研究 第4報 子のう殻の吸水と子のう胞子の放出との関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=1-2 article-no= start-page=23 end-page=32 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=196512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the mechanism of primary infection of wheat scab in relation to the environmental condition. V. Relationship between the climatic factors and the formation of perithecia in the field. kn-title=コムギ赤カピ病の第一次発生の伝染機構並びに環境条件に関する研究 (V) 自然状態における子のう殻の形成と気象条件 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue=1 article-no= start-page=43 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the effects of complexing agents for the toxicity mitigation and removal of heavy metals 3. Removal of metals from the Cd-accumulated carp by the exposure of complexing agents (EDTA) for short term (2 weeks) kn-title=コンプレキサンによる重金属の毒性緩和及び除去性に関する基礎的研究 第3報 EDTA短期間暴露によるカドミウム既汚染魚からの金属除去性について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=2 article-no= start-page=127 end-page=142 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197803 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the effects of complexing agents for the toxicity mitigation and removal of heavy metals. I. Effects of complexing agents (NTA, EDTA, DTPA) on accumulation and toxicity of sub-and lethal levels of heavy metals (Cu, Zn, Cu, Pb) to aquatic animals kn-title=コンプレキサンによる重金属の毒性緩和及び除去性に関する基礎的研究 第1報 重金属(Cd,Zn,Cu,Pb)の比較的高濃度水における水産動物への集積及び毒性に対するコンプレキサンの影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue=1 article-no= start-page=31 end-page=42 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the effects of complexing agents for the toxicity mitigation and removal of heavy metals 2. Effects of complexing agents on the toxicity of heavy metals to carp at low levels of the metals kn-title=コンプレキサンによる重金属の毒性緩和及び除去性に関する基礎的研究 第2報 低濃度の重金属(Cd,Cu)水における水生動物へのこれら重金属の集積及び毒性に対するコンプレキサンの影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=1 article-no= start-page=1 end-page=14 dt-received= dt-revised= dt-accepted= dt-pub-year=1971 dt-pub=197103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ゴボウに発生するウイルス(キク科植物のウイルスに関する研究 第2報) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=3 article-no= start-page=91 end-page=99 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=197203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ゴミムシダマシ類のぺンゾキノン含有と分泌 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=155 end-page=168 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Habenaria Mosaic Virus, A New Member of Potyvirus from Habenaria radiata Orchid kn-title=サギソウ(Habenaria radiata)から検出された新ひも状ウイルス,Habenaria Mosaic Potyvirus en-subtitle= kn-subtitle= en-abstract=1974年7月、市販されていたサギソウの葉に明瞭なモザイクを現わし、葉がややよじれ症状を呈した病原ウイルスを調べたところ、Potyvirusが検出された。本ウイルスは13科40種に汁液接種したところ、サギソウに全身感染し、C.amaranmnticolor、C.quinoa、ホウレンソウ、ツルナ、センニチコウの3科5種の植物に局部感染が認められた。本ウイルスはまたモモアカアブラムシ(Myzus persicae)によって非永続的に伝搬された。ウイルス粒子は長さ約750nm、幅約13nmのひも状であった。病葉粗液汁中の不活化限界は耐希釈性が10-5〜10-6、耐熱性が60〜65℃(10分)、耐希釈性が8〜16日(20℃)であった。純化ウイルスを家兎に注射して免疫し、力価512倍(微滴凝集反応法)の抗血清が得られた。本ウイルスおよびCIYVVに対する抗血清を用い、ELISA法により本ウイルス株、BYMV、CalMMV、DeMV、TuMV、WMV-2との血清反応試験、さらに本ウイルス、AlMV、BYMV、ClYVV、DeMV、FMV、PVY、TuMVとそれらのウイルスに対する抗血清との相互血清反応を免疫電顕法によって調べたところ、本ウイルスは血清学的に他の供試potyvirusとは異なるウイルスであることが認められた。電顕により観察したサギソウやC.quinoaなどの病葉組織の超薄切片像には細胞質内に風車状、車輪状、管状および層板状などの封入体の形成が認められた。また病葉からのPTA染色によるDN法試料には封入体の層板状の破片が認められ、それらの面には線間隔約5.2nmの平行微細構造が観察された。本ウイルスをhabenaria mosaic virus(HaMV)と命名し、和名をサギソウモザイクウイルス、サギソウの病名をモザイク病とした。HaMVは1991年5月にも同様葉にモザイクを生じた市販のサギソウからも検出された。 kn-abstract=A potyvirus, isolated from Habenaria radiaata showing severe mosaic in the leaves named habenaria mosaic virus(HaMV). HaMV was transmitted by inoculation of sap to 6 of 41 species from 4 of 12 families. Systemic infection was cauded in Habenaria radiata, and local infection was produced in Chenopodium amaranticolor, C. quinoa, Spinacia olracea, Tetragonia expansa and Gomphrena globosa. HaMV was also transmitted by Myzus persicae in a non persistent manner, but not by Aphis gossypii. The virus has filamentous particles of c. 750×13nm. Sap from inoculated C. quinoa was infective after dilution to 10-5 but not 10-6, after 10 min at 60℃ but 65℃, or after 8-16 days at 20℃.In ultrathin sections of diseased leaves of Habenaria radiata and inoculated C. quinoa, virus particles were found to disperse in the cytoplasm. Cylindrical inclusions were present in the cytoplasm of infected cells, as pinwheels, scrolls or tubes. The inclusions observed in negatively stained crude sap preparations exhibited fine linear striation at about 5.2 nm intervals.The antiserum to HaMV produced by immunizing a rabbit had a titer of 1/512(precipitin test). HaMV showed distant serological relationships to potyvirus;alstroemeria mosaic virus, bean yellow mosaic virus, calanthe mild mosaic virus, clover yellow vein virus, dendrobium mosaic virus, freesia mosaic virus, turnip mosaic virus and watermelon mosaic virus 2. en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=3 ORCID= en-aut-name=GaraI Wayan en-aut-sei=Gara en-aut-mei=I Wayan kn-aut-name=GaraI Wayan kn-aut-sei=Gara kn-aut-mei=I Wayan aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Habenaria orchid kn-keyword=Habenaria orchid en-keyword=Habenaria mosaic potyvirus kn-keyword=Habenaria mosaic potyvirus en-keyword=Habenaria mosaic disease kn-keyword=Habenaria mosaic disease END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=129 end-page=134 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Properties of α-Glucodidase from Taro Tuber kn-title=サトイモのα-グルコシダーゼの精製と性質 en-subtitle= kn-subtitle= en-abstract=里芋塊茎を破砕し、その抽出液からα−グルコシダーゼを硫安分画、エチルアルコール分画、CM−セルロファインカラムトグラフィー、調製用ディスク電気泳動により約2,500倍に精製した。本酵素はニゲロースに対してはマルトースと同程度に作用したが、イソマルトースに対する作用は非常に弱く、トレハロースにはまったく作用しなかった。本酵素は少糖類や多糖類にもよく作用し、マルトヘキサオースや可溶性澱粉に対してはマルトースと同程度の相対速度比と、むしろ低めのKm値を示した。このことから本酵素が生体内で澱粉分解に密接に関わっていることが推測された。 kn-abstract=α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose. en-copyright= kn-copyright= en-aut-name=MashimaHideyuki en-aut-sei=Mashima en-aut-mei=Hideyuki kn-aut-name=間島英之 kn-aut-sei=間島 kn-aut-mei=英之 aut-affil-num=1 ORCID= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=2 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=α-glucosidase kn-keyword=α-glucosidase en-keyword=taro tuber kn-keyword=taro tuber en-keyword=Colocasia esculanta Shott kn-keyword=Colocasia esculanta Shott END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=1 article-no= start-page=49 end-page=70 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological studies on breeding, feeding and hunting of the Japanese tree sparrow, Passer montanus saturatus STElNEGER kn-title=スズメPasser montanus saturatus STEJNEGERの繁殖,給餌ならびに探餌活動に関する生態学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WatabeN. en-aut-sei=Watabe en-aut-mei=N. kn-aut-name=渡部尚久 kn-aut-sei=渡部 kn-aut-mei=尚久 aut-affil-num=1 ORCID= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=3 article-no= start-page=171 end-page=184 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Turnip Mosaic Virus and Cucumber Mosaic Virus Infecting Statice (Limonium sinuatum) kn-title=スターチスに発生したturnip mosaic virus並ぴにcucumber mosaic virusについて en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IinoNaoyuki en-aut-sei=Iino en-aut-mei=Naoyuki kn-aut-name=飯野尚之 kn-aut-sei=飯野 kn-aut-mei=尚之 aut-affil-num=1 ORCID= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=3 article-no= start-page=209 end-page=223 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparison of Two Strains of Cucumber Mosaic Virus Isolated from a Single Statice Plant kn-title=スターチスの同一株から分離された違った病徴を発現する2種のcucumber mosaic virusについて en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=HuttingaHarm en-aut-sei=Huttinga en-aut-mei=Harm kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MitsuhataKōji en-aut-sei=Mitsuhata en-aut-mei=Kōji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=2 article-no= start-page=67 end-page=77 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=197503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The distribution of tall goldenrod (Solidago altissima L.) in Japan kn-title=セイタカアワダチソウ(Solidago altissima L.)の日本における分布 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakagawaK. en-aut-sei=Nakagawa en-aut-mei=K. kn-aut-name=中川恭二郎 kn-aut-sei=中川 kn-aut-mei=恭二郎 aut-affil-num=1 ORCID= en-aut-name=EnomotoT. en-aut-sei=Enomoto en-aut-mei=T. kn-aut-name=榎本敬 kn-aut-sei=榎本 kn-aut-mei=敬 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue=2 article-no= start-page=79 end-page=91 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197909 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological studies on Solidago altissima L. 2. Effect of planting density on the growth and reproduction kn-title=セイタカアワダチソウに関する生態学的研究 第2報 生長および繁殖に及ぼす密度効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=EnomotoT. en-aut-sei=Enomoto en-aut-mei=T. kn-aut-name=榎本敬 kn-aut-sei=榎本 kn-aut-mei=敬 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=1 article-no= start-page=13 end-page=21 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological Studies on Solidago altissima L. (3) Effects of light, temperature and watering conditions on the germination and establishment of the seedlings. kn-title=セイタカアワダチソウに関する生態学的研究 第3報 発芽および実生の生存と光,温度,水分条件との関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=EnomotoTakashi en-aut-sei=Enomoto en-aut-mei=Takashi kn-aut-name=榎本敬 kn-aut-sei=榎本 kn-aut-mei=敬 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=27 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sorghum Resistance to the Sugarcane Aphid, Melanaphis sacchari (Zehntenr) Amounts of Surface Wax and Nutritional Components kn-title=ソルガムのヒエノアブラムシ抵抗性 葉面ワックスと栄養成分 en-subtitle= kn-subtitle= en-abstract=ヒエノアブラムシの寄生密度にソルガム葉の葉面ワックス、栄養成分である糖と遊離アミノ酸がどのように影響するかを明らかにするために、抵抗性、中間、感受性の3系統のソルガムを用いて、これらの物量の含量を比較した。(1)ソルガムに寄生するアブラムシとしては、ヒエノアブラムシのみであった。8月中旬のアブラムシ寄生数は抵抗性系統で0、感受性系統で約3,500頭、その中間系統で約30頭であった。(2)葉面ワックス量は3系統ともほぼ同じであった。(3)全糖含量は抵抗性、中間系統の方が感受性系統に比べわずかに高い傾向であった。(4)全遊離アミノ酸含量は抵抗性、中間系統の方が、感受性系統よりも高かった。(5)これらの結果、葉面ワックス、糖、遊離アミノ酸はソルガムのアブラムシの寄生に影響を及ぼさないようにみえた。二次代謝産物であるデューリン、ベンジルアルコール、パラヒドロキシベンズアルデヒド、アコニット酸等が、ソルガムでのアブラムシの寄生密度を制御していると思われる。 kn-abstract=The differences in the amounts of leaf surface wax and nutritional components such as sugar and free amino acids were compared among resistant (PE 954177), moderately resistant (IS 84) and susceptible (Redlan B) sorghum varieties to the sugarcane aphid. Melanaphis sacchari (Zehntner). (1) The kind of aphid appearing on the leaves of the sorghum varieties was only the sugarcane aphid, Melanaphis sacchari in the field. The number of aphids infesting a leaf was ca 0,30 and 3,500 in the resistant, moderately resistant and susceptible varieties at the middle of August, respectively. (2) The amount of leaf surface wax was approximately constant among these three varieties. (3) The total sugar content was slightly higher in the two resistant varieties than in the susceptible variety. (4) The total free amino acid concentration was higher in the two resistant varieties than in the susceptible variety. (5) These findings suggest that neither leaf surface wax nor nutritional components seem to affect the aphid populations on the leaves of the three sorghum varieties, showing that biogenic substances such as dhurrin, benzylalcohol, p-hydroxybenzaldehyde, aconitic acide, etc. may affect the aphid populations. en-copyright= kn-copyright= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=MoharramipourSaeid en-aut-sei=Moharramipour en-aut-mei=Saeid kn-aut-name=MoharramipourSaeid kn-aut-sei=Moharramipour kn-aut-mei=Saeid aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Sorghum kn-keyword=Sorghum en-keyword=Sugarcane aphid kn-keyword=Sugarcane aphid en-keyword=Melanaphis sacchari kn-keyword=Melanaphis sacchari en-keyword=Leaf surface wax kn-keyword=Leaf surface wax en-keyword=Amino acid kn-keyword=Amino acid END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=1 article-no= start-page=29 end-page=37 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=198603 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Measurements of Turbulent Transport of Carbon Dioxide over a Sorghum Field kn-title=ソルガム群落上での炭酸ガスフラックスの測定 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OhtakiEiji en-aut-sei=Ohtaki en-aut-mei=Eiji kn-aut-name=大滝英治 kn-aut-sei=大滝 kn-aut-mei=英治 aut-affil-num=1 ORCID= en-aut-name=MaitaniToshihiko en-aut-sei=Maitani en-aut-mei=Toshihiko kn-aut-name=米谷俊彦 kn-aut-sei=米谷 kn-aut-mei=俊彦 aut-affil-num=2 ORCID= en-aut-name=SeoTakuro en-aut-sei=Seo en-aut-mei=Takuro kn-aut-name=瀬尾琢郎 kn-aut-sei=瀬尾 kn-aut-mei=琢郎 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=1 article-no= start-page=19 end-page=27 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=198603 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Measurement of Vertical Profiles and Fluctuations of Air Temperature within and above a Sorghum Canopy kn-title=ソルガム群落内外における平均気温の高度分布と気温変動の測定 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MaitaniToshihiko en-aut-sei=Maitani en-aut-mei=Toshihiko kn-aut-name=米谷俊彦 kn-aut-sei=米谷 kn-aut-mei=俊彦 aut-affil-num=1 ORCID= en-aut-name=SeoTakuro en-aut-sei=Seo en-aut-mei=Takuro kn-aut-name=瀬尾琢郎 kn-aut-sei=瀬尾 kn-aut-mei=琢郎 aut-affil-num=2 ORCID= en-aut-name=SahashiKen en-aut-sei=Sahashi en-aut-mei=Ken kn-aut-name=佐橋謙 kn-aut-sei=佐橋 kn-aut-mei=謙 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=4 article-no= start-page=221 end-page=225 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A simple method for mass rearing of the seed-corn maggot, Hylemya platura MEIGEN (Diptera: Anthomyiidae) kn-title=タネバエHylemya platura Meigenの簡易大量飼育法 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MatsumotoY. en-aut-sei=Matsumoto en-aut-mei=Y. kn-aut-name=松本義明 kn-aut-sei=松本 kn-aut-mei=義明 aut-affil-num=1 ORCID= en-aut-name=SugiyamaS. en-aut-sei=Sugiyama en-aut-mei=S. kn-aut-name=杉山章平 kn-aut-sei=杉山 kn-aut-mei=章平 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=145 end-page=158 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=19690326 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the relations between the seedling stage of Raphanus sativus L. and vernalization V. Relation between the growth of shoot apex and the sensitivity to vernalization in the seedling stage of Raphanus sativns L. kn-title=ダイコンの発芽期とVernalizationとの関係に関する研究 第5報 生長点の生長と感温性の関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NishiK. en-aut-sei=Nishi en-aut-mei=K. kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=3 article-no= start-page=123 end-page=138 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19681015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the relations between the seedling stage of Raphanus sativus L. and vernalization III. Features of the shoot apex in the seedling stage of Raphanus sativus L. kn-title=ダイコンの発芽期とVernalizationとの関係に関する研究 第3報 発芽期の生長点の形態 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NishiK. en-aut-sei=Nishi en-aut-mei=K. kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=3 article-no= start-page=139 end-page=144 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19681015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the relations between the seedling stage of Raphanus sativus L. and vernalization IV. Changes of polysaccharide, protein and nucleic acid in the shoot apex in the seedling stage of Raphanus sativus L. kn-title=ダイコンの発芽期とVernalizationとの関係に関する研究 第4報 発芽期の生長点の多糖類, 蛋白質, 核酸の消長 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NishiK. en-aut-sei=Nishi en-aut-mei=K. kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=4 article-no= start-page=205 end-page=212 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=196003 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effect of population density on the wing development of the cabbage aphid, Brevicoryne brassicae L. kn-title=ダイコンアブラムシBrevicoryne brassicae L.の有翅型出現に及ぼす飼育密度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=15 end-page=19 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ダイコンアブラムシBrevicoryne brassicae L.の有翅型出現におよぽす密度効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=21 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ダイコンアブラムシBrevicoryne brassicae L.の有翅型出現におよぼす飼育密度,日長時間,環境温度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=131 end-page=136 dt-received= dt-revised= dt-accepted= dt-pub-year=1962 dt-pub=196203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological studies on the cabbage aphis, Brevicoryne brassicae L. III. The relation between the period of wing development and the high population density. kn-title=ダイコンアプラムシの生態学的研究 第3報 有翅型出現の時期と集合飼育との関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=2 article-no= start-page=79 end-page=90 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=197203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ダリヤに発生したTomato spotted wilt virus(キク科植物のウイルスに関する研究 第3報) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=3 article-no= start-page=185 end-page=193 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Changes in Distribution of α-Glucosidases in Rice cells Grown with Tunicamycin kn-title=ツニカマイシン存在下での培養における水稲α−グルコシダーゼの細胞内分布の変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=1 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=2 article-no= start-page=69 end-page=80 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A strain of cucumber mosaic virus isolated from lily kn-title=テッポウユリから分離されたキュウリモザイクウイルスの性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MaedaT. en-aut-sei=Maeda en-aut-mei=T. kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=3-4 article-no= start-page=203 end-page=214 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Calcium deficiency symptom in maize and sorghum. I. Occurrence of a characteristic symptom in plant leaves (1.Maize) kn-title=トウモロコシやソルガムなどに出現するカルシウム欠乏症状(第1報) 特異な欠乏症状の発現(1.トウモロコシ) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawasakiT. en-aut-sei=Kawasaki en-aut-mei=T. kn-aut-name=河崎利夫 kn-aut-sei=河崎 kn-aut-mei=利夫 aut-affil-num=1 ORCID= en-aut-name=MoritsuguM. en-aut-sei=Moritsugu en-aut-mei=M. kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=3-4 article-no= start-page=215 end-page=223 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Calcium deficiency symptom in maize and sorghum. II. Occurrence of a characteristic symptom in plant leaves (2.Sorghum and others) kn-title=トウモロコシやソルガムなどに出現するカルシウム欠乏症状(第2報) 特異な欠乏症状の発現(2.ソルガム,その他) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawasakiT. en-aut-sei=Kawasaki en-aut-mei=T. kn-aut-name=河崎利夫 kn-aut-sei=河崎 kn-aut-mei=利夫 aut-affil-num=1 ORCID= en-aut-name=MoritsuguM. en-aut-sei=Moritsugu en-aut-mei=M. kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=4 article-no= start-page=187 end-page=200 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=198503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A characteristic symptom of calcium deficiency in maize and sorghum. III. Some factors related to development of the symptom kn-title=トウモロコシやソルガムなどに出現するカルシウム欠乏症状(第3報) トウモロコシにおける特異なカルシウム欠乏症状の発現要因について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MoritsuguM. en-aut-sei=Moritsugu en-aut-mei=M. kn-aut-name=森次益三 kn-aut-sei=森次 kn-aut-mei=益三 aut-affil-num=1 ORCID= en-aut-name=KawasakiT. en-aut-sei=Kawasaki en-aut-mei=T. kn-aut-name=河崎利夫 kn-aut-sei=河崎 kn-aut-mei=利夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=159 end-page=164 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=19690326 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Peanut mottle virus from peanut and pea kn-title=ナンキンマメおよびエンドウに発生するナンキンマメ斑紋ウイルス(Peanut mottle virus) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeT. en-aut-sei=Inouye en-aut-mei=T. kn-aut-name=井上忠男 kn-aut-sei=井上 kn-aut-mei=忠男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=101 end-page=106 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Pathways of glycogen metabolism in larvae of the rice stem borer, Chilo suppressalis Walker kn-title=ニカメイガにおけるグリコーゲン代謝系路について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsumukiH. en-aut-sei=Tsumuki en-aut-mei=H. kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=ShiragaT. en-aut-sei=Shiraga en-aut-mei=T. kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=4 article-no= start-page=195 end-page=204 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=196003 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The influence of temperature on the embryonic development of the rice stem borer moth, Chilo suppressalis walker. kn-title=ニカメイガ卵の発育と温度との関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SugiyamaS. en-aut-sei=Sugiyama en-aut-mei=S. kn-aut-name=杉山章平 kn-aut-sei=杉山 kn-aut-mei=章平 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page=31 end-page=40 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=197303 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The carbohydrate contents and oxygen consumption in the hybernating larvae of the Rice Stem-Borer, Chilo suppressalis WALKER kn-title=ニカメイガ越冬幼虫の炭水化物含量と酸素吸収量について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsumukiH. en-aut-sei=Tsumuki en-aut-mei=H. kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=2 article-no= start-page=79 end-page=86 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=197503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Metabolism of 14C-glucose and 14C-UDPG in the non-diapause larvae of the rice stem borer, Chilo suppressalis WALKER, in vivo kn-title=ニカメイガ非休眠幼虫における14C-glucoseと14C-UDPG en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsumukiH. en-aut-sei=Tsumuki en-aut-mei=H. kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=1 article-no= start-page=1 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=1963 dt-pub=196303 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the geographical distribution of the 28-spotted lady beetles, Epilachna sparsa orientalis Dieke and Epilachna vigintioctomaculata Motschulsky in Japan. kn-title=ニジュウヤホシテントウEpilachna sparsa orientalis DIEKEとオオニジュウヤホシテントウEpilachna vigintioctomaculata MOTSCHULSKYの地理的分布に関する調査研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=1 article-no= start-page=45 end-page=47 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=New record of adult males in the life cycle of Lipaphis erysimi KALTENBACH (Aphididae) from Japan kn-title=ニセダイコンアブラムシLipaphis erysimi KALTENBACHの雄虫出現について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuraiT. en-aut-sei=Murai en-aut-mei=T. kn-aut-name=村井保 kn-aut-sei=村井 kn-aut-mei=保 aut-affil-num=1 ORCID= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=2 article-no= start-page=87 end-page=92 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197803 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Occurrence of mulberry trees,Morus sp. in New Guinea (New Guinea Studies. II.) kn-title=ニューギニアの桑について (ニューギニア研究 第2報) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=91 end-page=98 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ネパール産大麦系統および大麦品種“トルコ290”のうどんこ病に対する抵抗性遺伝子 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=日浦運治 kn-aut-sei=日浦 kn-aut-mei=運治 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=河龍雄 kn-aut-sei=河 kn-aut-mei=龍雄 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=部田英雄 kn-aut-sei=部田 kn-aut-mei=英雄 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=2 article-no= start-page=81 end-page=89 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Formation of glucosylriboflavin from riboflavin in germinating barley seeds kn-title=ハダカムギの発芽種子におけるリボフラビンからグルコシルリボフラビンの生成 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SuzukiY. en-aut-sei=Suzuki en-aut-mei=Y. kn-aut-name=鈴木幸雄 kn-aut-sei=鈴木 kn-aut-mei=幸雄 aut-affil-num=1 ORCID= en-aut-name=UchidaK. en-aut-sei=Uchida en-aut-mei=K. kn-aut-name=内田絅 kn-aut-sei=内田 kn-aut-mei=絅 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=3 article-no= start-page=111 end-page=121 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198403 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Defensive secretory organs of the rove beetles (Coleoptera: Staphylinidae) kn-title=ハネカクシ類の防御物質分泌期間 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=ShiragaT. en-aut-sei=Shiraga en-aut-mei=T. kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=2 ORCID= en-aut-name=KawazuK. en-aut-sei=Kawazu en-aut-mei=K. kn-aut-name=河津一義 kn-aut-sei=河津 kn-aut-mei=一義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=1-2 article-no= start-page=69 end-page=80 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ヒエおよびイネの発芽に対する2,3除草剤の作用の差異について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=5 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Host Selection, Development and Reproduction of Melanaphis sacchari (Zehntner) kn-title=ヒエノアブラムシ  Melanaphis sacchari(Zehntner)の寄生選択,発育および繁殖に関する研究 en-subtitle= kn-subtitle= en-abstract=ヒエノアブラムシの寄主選択行動と発育、繁殖について、温度25℃、16時間日長条件下で調査し、次のような結果を得た。有翅胎生雌虫、無翅胎生雌虫の成虫は感受性ソルガム(Redlan B)と野生のセイバンモロコシに対して強い選好性を示した。抵抗性ソルガム(P.E.954-177)は、感受性ソルガム(Redlan B)に比較する幼虫期間の遅延、産子数の低下および生存期間の短縮がみられた。 kn-abstract=The host selection, development and reproduction of sugarcane aphid, Melanaphis sacchari (Zehntner) were studied. Both alate and apterous virginoparous adults showed a stronger tendency of preference to a susceptible strain of sorghum and Cuba grass, Sorghum helepense (Persoon) than a resistant strain of sorghum and Eulalia, Miscanthus sinensis (Anderss). The number of days for larval development was increased with a decrease in longevity and fecundity in aphids reared on resistant sorghum at 25℃ under a 16-hour photoperiodic condition. en-copyright= kn-copyright= en-aut-name=KawadaKazuo en-aut-sei=Kawada en-aut-mei=Kazuo kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=Sorghum kn-keyword=Sorghum en-keyword=Host selection kn-keyword=Host selection en-keyword=Sugarcane aphids kn-keyword=Sugarcane aphids END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=131 end-page=136 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Effect on Germination of Some Species of the Genus Echinochloa and Rice Seeds with Various Osmotic Pressure of Salt, Sugar, and PEG Solutions kn-title=ヒエ属植物およびイネの種子の発芽に及ぼす塩分、蔗糖、PEG溶液の浸透圧の影響 en-subtitle= kn-subtitle= en-abstract=干拓地、塩害地における雑草の発生、植物の対塩生機構の解明の一端として、水田強害草の野生ヒエの塩分に対する発芽反応の研究を行った。水田で発芽生育する作物のイネは塩分による発芽の阻害は浸透圧の増大によるところが大きいことを示した。一方、雑草のヒエは概して浸透圧による発芽の阻害は塩分、蔗糖によって回復される傾向を示し、特に変温条件下の塩分でイヌビエ種の発芽の阻害が相当程度回復することを示した。これは野生ヒエ、特にイヌビエが自然状態の塩分の比較的多いところにでもそれに適応して発芽可能なことを暗示しているものと考えられる。 kn-abstract=To investigate the differences and mechanism of germination among the seeds of some species of genus Echinochloa and rice, the experiments were conducted in solutions of salt, sugar, and PEG(poly-ethylene glycol) under a constant or alternating temperature in the light or the dark. The seeds of Echinochloa oryzicola Vasing., Ehinochloa crus-galli Beauv.var. formosensis Ohwi, Echinochloa crus-galli Beauv.var.crus-galli(awnless and awned type)and Oryza sativa L.(Akebono) were used. In 7000hPa, germination of rice,E. oryzicola and three varieties of E.crus-galli in all the solutions remarkedly decreased except for the case of E.crus-galli in the solution of sale on alternating temperature. The germination of rice and E.oryzicola was secreased markedly, and more markedly in a light condition than in a dark. In the salt solution in 7000hPa, the decreasing of germination of E.crus-galli was low. This species is distributed and grows in the upland and paddy field, while E.oryzicola and rice grow only in the paddy field. The decreade in germination of Echinochloa species with osmotic pressure seemed to be recoverde with salt or sugar solutions under alternating temperature, but the recovery of rice was not seen. In 700hPa or less, the distinct differences of germination were not seen among these plants species. en-copyright= kn-copyright= en-aut-name=NishiKatsuhisa en-aut-sei=Nishi en-aut-mei=Katsuhisa kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 en-keyword=Genus Echinochloa kn-keyword=Genus Echinochloa en-keyword=Germination kn-keyword=Germination en-keyword=Osmotic pressure kn-keyword=Osmotic pressure END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue=3-4 article-no= start-page=181 end-page=190 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Occurrence of branched ears at high nodal position of stems in barnyardgrass. Echinochloa crus-galli var. formosensis kn-title=ヒメタイヌビエにおける高節位分けつ穂の発生について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NishiK. en-aut-sei=Nishi en-aut-mei=K. kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=4 article-no= start-page=227 end-page=243 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Viruses causing Mosaic and Flower Color Breaking Diseases on Zinnia elegans kn-title=ヒャクニチソウのモザイクとカラーブレーキング病の病原ウイルス en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=2 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=145 end-page=165 dt-received= dt-revised= dt-accepted= dt-pub-year=1962 dt-pub=196203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Observations on the micrometeorology of vinyl-houses (polyethylene-covered nursery beds). kn-title=ビニール・ハウスの微気象観測例 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SeoT. en-aut-sei=Seo en-aut-mei=T. kn-aut-name=瀬尾琢郎 kn-aut-sei=瀬尾 kn-aut-mei=琢郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=43 end-page=48 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Relationship between Resistance to Cereal Aphids and Gramine Concentration in Beer Barley kn-title=ビールムギのアブラムシ抵抗性とグラミン含量 en-subtitle= kn-subtitle= en-abstract=ビールムギを含む各種オオムギへのアブラムシの寄生数と、グラミン含量に注目して実験を行った。用いたビールムギは他のオオムギに比べ、アブラムシに比較的抵抗性を示すものが多く、極端な感受性を示す品種はなかった。特に、アサヒ5号やハルナ2条は野生系統H603(W603)に近い抵抗性を示した。ビールムギのグラミン含量はいずれも低く、アブラムシの寄生密度との間には負の相関が見られなかった。 kn-abstract=To beer barley resistance to cereal aphids, the relationship between the aphid density and gramine concentration was examined in various barley lines including beer barley cultivars. All beer barley cultivars tested were resistant or moderately resistant to the aphids, especially Asahi 5 and Haruna Nijo were similar to a wild line, H603 (W603), for checking resistant. However, these beer barleys contained much less gramine than the wild barley line, suggesting that another resistant factor(s) affects cereal aphid density. en-copyright= kn-copyright= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KasaharaToshihiko en-aut-sei=Kasahara en-aut-mei=Toshihiko kn-aut-name=笠原敏彦 kn-aut-sei=笠原 kn-aut-mei=敏彦 aut-affil-num=2 ORCID= en-aut-name=KanehisaKatsuo en-aut-sei=Kanehisa en-aut-mei=Katsuo kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=3 ORCID= en-aut-name=SaeidMoharramipour en-aut-sei=Saeid en-aut-mei=Moharramipour kn-aut-name=SaeidMoharramipour kn-aut-sei=Saeid kn-aut-mei=Moharramipour aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Beer barley kn-keyword=Beer barley en-keyword=Cereal aphid kn-keyword=Cereal aphid en-keyword=Resistance kn-keyword=Resistance en-keyword=Gramine kn-keyword=Gramine END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=39 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the chemical control of white root-rot of vines. I. First screening test with fungicides in the liquid form. kn-title=ブドウ白紋羽病の薬剤防除に関する研究 第1報 薬剤(液剤)スクリーニングテスト en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamauchiK. en-aut-sei=Yamauchi en-aut-mei=K. kn-aut-name=山内己酉 kn-aut-sei=山内 kn-aut-mei=己酉 aut-affil-num=1 ORCID= en-aut-name=ShiomiM. en-aut-sei=Shiomi en-aut-mei=M. kn-aut-name=塩見正保 kn-aut-sei=塩見 kn-aut-mei=正保 aut-affil-num=2 ORCID= en-aut-name=OkamotoY. en-aut-sei=Okamoto en-aut-mei=Y. kn-aut-name=岡本康博 kn-aut-sei=岡本 kn-aut-mei=康博 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=2 article-no= start-page=79 end-page=102 dt-received= dt-revised= dt-accepted= dt-pub-year=1964 dt-pub=196403 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of mulchings with plastic films on intensity of illumination, temperature, moisture and pH of soil, growth of lettuce and weed control. kn-title=プラスチックフィルムのマルチによる照度,温度,土壤水分,土壤pHの変化とレタスの生育および雑草防除との関係 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KasaharaY. en-aut-sei=Kasahara en-aut-mei=Y. kn-aut-name=笠原安夫 kn-aut-sei=笠原 kn-aut-mei=安夫 aut-affil-num=1 ORCID= en-aut-name=NishiN. en-aut-sei=Nishi en-aut-mei=N. kn-aut-name=西克久 kn-aut-sei=西 kn-aut-mei=克久 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=1 article-no= start-page=17 end-page=23 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Fractionation of polysaccharides, acidic oligosaccharides and neutral sugars formed on partial acid hydrolysis of pectin kn-title=ペクチン質の酸部分分解によって生成する高分子多糖, 酸性オリゴ糖, 中性糖の分画 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonnoH. en-aut-sei=Konno en-aut-mei=H. kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=1 ORCID= en-aut-name=YamasakiY. en-aut-sei=Yamasaki en-aut-mei=Y. kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=2 ORCID= en-aut-name=OzawaJ. en-aut-sei=Ozawa en-aut-mei=J. kn-aut-name=小沢潤二郎 kn-aut-sei=小沢 kn-aut-mei=潤二郎 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=239 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Characterization of α-Glucosidases from Spinach Seeds kn-title=ホウレンソウ(Spinacia oleracea L.)種子のαグルコシダーゼの精製とその性質 en-subtitle= kn-subtitle= en-abstract=α−グルコシダーゼの分子多型が各種クロマトグラフィーによりホウレンソウ種子から単離された。α−グルコシダーゼT、U、V、Wの分子量は、それぞれSDS−PAGEにより78、78、82、82kDA、ゲル濾過のより62,62,190、70kDであった。α-グルコシダーゼTとUは、可溶性デンプンを基質とした際のKm値がマルトースよりも約10倍低く、さらに、α−グルカンだけでなくマルトオリゴ糖に対しても高い活性を示すことから、同じような酵素的特性を持つことが示された。最適pHは4.5カラ5.5を示し、温度安定性は70℃、20分処理で約50%の残存活性を示した。一方、α−グルコシダーゼVとWは、マルトースを基質とした際のKm値が可溶性デンプンよりも3−4倍低い値を示し、マルトオリゴ糖に対しては高い活性を示すが、α−グルカンに対してはほとんど活性を示さないことから、同じような酵素的特性を有することが示された。最適pHは4.5−5.5、そして70℃、20分処理では活性が認められなかった。しかしながら、抗α−グルコシダーゼV血清は、α−グルコシダーゼVに対してのみ特異的に沈降線を形成した。 kn-abstract=Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases T,U,V,and W were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectively. α-Glucosidases Tand U showed similar enzymatic properties. The Km for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only towards malto-oligosaccharides but also towards α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 71℃ for 20 min. On the other hand, α-glucosidases V and W showed similar enzymatic propreties. The Km for maltose was 3-4 times lower than for solble starch, and they had high activity toward malto-oligosaccharides but faint activity towards α-glucnas. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20 min. However, anti-α-glucosidase V serum precipitated specifically with α-glucosidase V. en-copyright= kn-copyright= en-aut-name=SugimotoManabu en-aut-sei=Sugimoto en-aut-mei=Manabu kn-aut-name=杉本学 kn-aut-sei=杉本 kn-aut-mei=学 aut-affil-num=1 ORCID= en-aut-name=FuruiSatoshi en-aut-sei=Furui en-aut-mei=Satoshi kn-aut-name=古井聡 kn-aut-sei=古井 kn-aut-mei=聡 aut-affil-num=2 ORCID= en-aut-name=SuzukiYukio en-aut-sei=Suzuki en-aut-mei=Yukio kn-aut-name=鈴木幸雄 kn-aut-sei=鈴木 kn-aut-mei=幸雄 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=α-Glucosidase kn-keyword=α-Glucosidase en-keyword=Spinach kn-keyword=Spinach en-keyword=Seed kn-keyword=Seed en-keyword=Spinacia oleracea L. kn-keyword=Spinacia oleracea L. en-keyword=Molecular form kn-keyword=Molecular form END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=3 article-no= start-page=181 end-page=188 dt-received= dt-revised= dt-accepted= dt-pub-year=1970 dt-pub=197001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=マツタケ発生林内微気象観測例 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=高須謙一 kn-aut-sei=高須 kn-aut-mei=謙一 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=木村和義 kn-aut-sei=木村 kn-aut-mei=和義 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=則武赳夫 kn-aut-sei=則武 kn-aut-mei=赳夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=4 article-no= start-page=195 end-page=215 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197703 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological studies on water nutsedge (Cyperus serotinus Rottb.) II. The methods of cultural control kn-title=ミズガヤツリの防除に関する生態学的研究 II. 耕種的防除法について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakagawaK. en-aut-sei=Nakagawa en-aut-mei=K. kn-aut-name=中川恭二郎 kn-aut-sei=中川 kn-aut-mei=恭二郎 aut-affil-num=1 ORCID= en-aut-name=HattoriK. en-aut-sei=Hattori en-aut-mei=K. kn-aut-name=服部金次郎 kn-aut-sei=服部 kn-aut-mei=金次郎 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=58 cd-vols= no-issue=2 article-no= start-page=51 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197909 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological studies on water nutsedge (Cyperus serotinus Rottb.) III. The intraspecific variations kn-title=ミズガヤツリの防除に関する生態学的研究 III. 種内変異について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakagawaK. en-aut-sei=Nakagawa en-aut-mei=K. kn-aut-name=中川恭二郎 kn-aut-sei=中川 kn-aut-mei=恭二郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=1 article-no= start-page=15 end-page=31 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ecological studies on water nutsedge (Cyperus serotinus Rottb.) I. Individual ecological characteristics kn-title=ミズガヤツリの防除に関する生態学的研究 T 個体生態について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakagawaK. en-aut-sei=Nakagawa en-aut-mei=K. kn-aut-name=中川恭二郎 kn-aut-sei=中川 kn-aut-mei=恭二郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=3 article-no= start-page=177 end-page=189 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Method for Mass Sporulation in a Scab Disease Pathogen (Fusarium graminearum Schwabe) kn-title=ムギ頬赤かび病菌(Fusarium graminearum Schwabe)における分生子の大量培養法 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KanataniRyoichi en-aut-sei=Kanatani en-aut-mei=Ryoichi kn-aut-name=金谷良市 kn-aut-sei=金谷 kn-aut-mei=良市 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=23 end-page=31 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the mechanism of prevalence of wheat and barley scab caused by Gibberella zeae (Schw.) Petch. III. kn-title=ムギ類アカカビ病の流行機構に関する研究 第III報 発生型と予察(1) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IshiiH. en-aut-sei=Ishii en-aut-mei=H. kn-aut-name=石井博 kn-aut-sei=石井 kn-aut-mei=博 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=1 article-no= start-page=47 end-page=60 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=1997 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Mutation of Fungicide Tolerance in Fusarium spp. kn-title=ムギ類赤かび病菌における薬剤耐性の突然変異 en-subtitle= kn-subtitle= en-abstract=ムギ類赤かび病菌のトップジンに対する耐性菌の出現頻度は10-5〜10-6程度で、培養日数、紫外線照射、トップジン添加処理などによって一定の変化は示されず、自然突然変異とみられた。赤かび病菌104菌株を供試して培地のトップジン濃度を順次に高めながら高度耐性菌株を選抜した結果、様々な濃度のトップジンに耐性を持つ菌株が作出され、その中にはトップジン40,000ppmを含む培地で菌糸が伸長し、トップジンに対する感受性を失ったとみられる菌株も見出された。トップジン耐性は菌糸、分生子ならびに子のう胞子を通じて伝達されることが確認された。耐性菌と原菌株の菌糸の伸長速度、分生子形成能力および病原力を比較したところ、菌糸の伸長速度はいずれの温度条件でも耐性菌の方が有意に遅くなったが、分生子形成能力及び病原力には有意な差が認められなかった。また、耐性菌にはトップジンによる防除効果が認められなかった。トップジン耐性菌38菌株を赤かび病菌に効果が高い5種類の殺菌剤(トップジン、べフラン、キノンドー、スポルタックおよびベンレート)と効果が低い2種類の殺菌剤(ダコニールおよびカラセン)で処理して交差耐性を調べた結果、トップジン耐性とベンレート耐性はほぼ完全一致(R=0.952)したが、他の差菌剤とは交差耐性を示さなかった。 kn-abstract=Strains of Fusarium, causal fungi of scab disease of cereal crops, were examined for the mutation of topsin tolerance. The mutation rate of topsin tolerant spores was 10-5~10-6 and was not affected by the irradiation of ultraviolet rays nor application of topsin to the culture medium. Highly tolerant strains were easily selected on the screening medium containing topsin. Finally topsin was not effective to the selected tolerant mutants. The topsin tolerance was parallel to "benrate" tolerance. The tolerance was transmitted through hyphae, conidium and ascospore. The growth rate of hyphae of the tolerant mutants was lower than that of the original strains, but the conidia formation and the virulence of the mutants were comparable to the original strains. Since the heavy application of fungicides may increase the fungicide-tolerant mutants, crop varieties resistant to scab disease must be developed. en-copyright= kn-copyright= en-aut-name=WuJiri en-aut-sei=Wu en-aut-mei=Jiri kn-aut-name=呉基日 kn-aut-sei=呉 kn-aut-mei=基日 aut-affil-num=1 ORCID= en-aut-name=TakedaKazuyoshi en-aut-sei=Takeda en-aut-mei=Kazuyoshi kn-aut-name=武田和義 kn-aut-sei=武田 kn-aut-mei=和義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Fusarium spp. kn-keyword=Fusarium spp. en-keyword=Fungicide tolerance kn-keyword=Fungicide tolerance en-keyword=Mutation. kn-keyword=Mutation. END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=4 article-no= start-page=199 end-page=206 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=197303 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=メタクレゾールを分泌するゴミムシ類 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=1 article-no= start-page=5 end-page=11 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of Burial Conditions on Seedling Emergence of Crabgrass (Digitaria adscendens Henrard) kn-title=メヒシバ種子の越冬時の埋土位置が畑地での出芽動態に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsuyuzakiHiroshi en-aut-sei=Tsuyuzaki en-aut-mei=Hiroshi kn-aut-name=露崎浩 kn-aut-sei=露崎 kn-aut-mei=浩 aut-affil-num=1 ORCID= en-aut-name=NakagawaKyojiro en-aut-sei=Nakagawa en-aut-mei=Kyojiro kn-aut-name=中川恭二郎 kn-aut-sei=中川 kn-aut-mei=恭二郎 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=1 article-no= start-page=59 end-page=65 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Population Increases of the Green Peach Aphid, Myzus persicae (Sulzer) and Cotton Aphid, Aphis gossypii Glover kn-title=モモアカアブラムシとワタアブラムシの個体群増殖 en-subtitle= kn-subtitle= en-abstract=モモアカアブラムシとワタアブラムシについて、それぞれダイコンとキュウリを餌植物として、一連の温度条件での個体群増殖のパラメータを求めた。産子数はアカアブラムシの方が多かった。しかし、生存期間はワタアブラムシの方が長かった。羽化後の日別産子数と日別生存率から求めた純繁殖率と内的自然増加率は、モモアカアブラムシでは、15℃で、61.50と0.21、20℃で60.60と0.33であった。ワタアブラムシでは、15℃で37.90と0.22、20℃で45.70と0.32、25℃で40.60と0.42であった。 kn-abstract=The reproduction of Myzus persicae (Sulz.) on raddish and Aphis gossypii Glover on cucumber was studied at different temperatures. The parameters of population growth in these aphids were calculated by the age-specific fecundities (mx) and survival rate (lx). The net reproductive rate (R0) and intrinsic rate of natural increase (r) of M. persicae were 61.50 and 0.21 at 15℃, and 60.60 and 0.33 at 20℃, respectively. The net reproductive rate (R0) and intrinsic rate of natural increase (r) of A. gossypii were 37.90 and 0.22 at 15℃, 45.70 and 0.32 at 20℃, and 40.60 and 0.42 at 25℃, respectively. en-copyright= kn-copyright= en-aut-name=MuraiTamotsu en-aut-sei=Murai en-aut-mei=Tamotsu kn-aut-name=村井保 kn-aut-sei=村井 kn-aut-mei=保 aut-affil-num=1 ORCID= en-aut-name=TsumukiHisaaki en-aut-sei=Tsumuki en-aut-mei=Hisaaki kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Population growth kn-keyword=Population growth en-keyword=Net reproductive rate kn-keyword=Net reproductive rate en-keyword=Intrinsic rate kn-keyword=Intrinsic rate en-keyword=Myzus persicae kn-keyword=Myzus persicae en-keyword=Aphis gossypii kn-keyword=Aphis gossypii END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=141 end-page=148 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Attractiveness of umbelliferous plants to the vegetable weevil. Studies on the host plant determination of the leaf-feeding insects. III. kn-title=ヤサイゾウムシに対するセリ科植物の誘引性 食葉性昆虫の寄主決定に関する研究 III en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SugiyamaS. en-aut-sei=Sugiyama en-aut-mei=S. kn-aut-name=杉山章平 kn-aut-sei=杉山 kn-aut-mei=章平 aut-affil-num=1 ORCID= en-aut-name=MatsumotoY. en-aut-sei=Matsumoto en-aut-mei=Y. kn-aut-name=松本義明 kn-aut-sei=松本 kn-aut-mei=義明 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1-2 article-no= start-page=114 end-page=122 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=195911 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of temperature and humidity on the life of the vegetable weeivil, Listroderes costirostris obliquus KLUG. kn-title=ヤサイゾウムシの発育に及ぼす温湿度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=1 ORCID= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=1-2 article-no= start-page=129 end-page=133 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=196512 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Trends in the water and lipid content and in the body fluid refractive index of the vegetable weevil adults during the first feeding stage and diapause period. kn-title=ヤサイゾウムシ成虫の初期摂食活動期・休眠期における水分・脂質量・体液屈折率の変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MatsumotoY. en-aut-sei=Matsumoto en-aut-mei=Y. kn-aut-name=松本義明 kn-aut-sei=松本 kn-aut-mei=義明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=4 article-no= start-page=171 end-page=179 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=198503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effect of photoperiod and temperature on the production of sexual forms of Lipaphis erysimi Kaltenbach kn-title=ユセダイコンアブラムシLipaphis erysimi Kaltenbachの有性虫出現に及ぼす日長と温度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=3 article-no= start-page=135 end-page=146 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198403 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A distinctive strain of tulip breaking virus isolated from lilies in Japan kn-title=ユリから分離されたチューリップモザイクウイルスの1系統 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MaedaT. en-aut-sei=Maeda en-aut-mei=T. kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=1 ORCID= en-aut-name=InouyeN. en-aut-sei=Inouye en-aut-mei=N. kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=2 ORCID= en-aut-name=MitsuhataK. en-aut-sei=Mitsuhata en-aut-mei=K. kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=62 cd-vols= no-issue=4 article-no= start-page=253 end-page=270 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Iron Forms Influencing the Growth and Munty-Odor Production of Cyanobacteria (Oscillatoria brevis, Anabaena macrospora, Phormidium tenue and Oscillatoria tenuis) kn-title=ラン藻類Oscillatoria brevis, Anabaena macrospora, Phormidium tenue及びOscillatoria tenuisの増殖とかび臭物質産生に必要な鉄の形態 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakashimaSusumu en-aut-sei=Nakashima en-aut-mei=Susumu kn-aut-name=中島進 kn-aut-sei=中島 kn-aut-mei=進 aut-affil-num=1 ORCID= en-aut-name=YagiMasakazu en-aut-sei=Yagi en-aut-mei=Masakazu kn-aut-name=八木正一 kn-aut-sei=八木 kn-aut-mei=正一 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END