ID | 13854 |
Eprint ID | 13854
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FullText URL | |
Title Alternative | Effect of zinc on halothane-induced liver injury in rats
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Author |
Hidaka, Hidekuni
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Abstract | In a rat model, halothane causes liver injury by reductive interaction with microsomal cytochrome P-450 (P-450) and /or by a hepatotoxic effect mediated by halothane-derived free radicals. Rats that were pretreated with phenobarbital (0.1% in drinking water for 6 days) and fasted for the last day, then exposed to halothane (1.0%) under reduced oxygen tension (14%) for 2 horus developed hepatic centrilobular necrosis with marked elevation in serum GPT (GPT) 24 h after exposure. Pretreatment with a 5 mg/kg dose of zinc (Zn) 24 h prior to the exposure had no effect on GPT and liver necrosis. However, 10mg/kg and 20 mg/kg of Zn significantly decreased GPT and liver necrosis. Zn-pretreatment (5-20 mg/kg) significantly depleted hepatic microsomal P-450 before exposure in a dose dependent manner. Hepatic metallothionein (MT)-1 and MT-2 induced by Zn in a dose dependent manner and the levels did not significanlty differ prior to and after exposure to halothane under hypoxic conditions in all Zn-pretreated groups of rats. These results indicated that Zn-pretreatment has some protective effect against halothane-induced liver injury and suggested that this protective effect of Zn involves the depletion of P-450 which results in reduced interacton between P-450 and halothane in the microsomes and is not the result of MTs acting as free radical scavengers.
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Keywords | ハロタン
肝障害
亜鉛
チトクローム P-450
メタロチオネイン
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Note | 原著
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Published Date | 1994-10
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume106
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Issue | issue9-10
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 1073
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End Page | 1084
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/106/9-10/106_9-10_1073/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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