start-ver=1.4
cd-journal=joma
no-vol=85
cd-vols=
no-issue=9
article-no=
start-page=957
end-page=961
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201209
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Electroconductive ƒÎ-Junction Au Nanoparticles
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The fabrication of printed electronic circuits using solution-based electroconductive materials at low temperature is essential for the realization of modern printed electronics including transistors, photovoltaic cells, and light-emitting devices. Despite the progress in the field of semiconductor solution materials, reliable electrodes are always fabricated by a vacuum deposition process resulting in only partially solution-processed devices. In this paper, we show that planar phthalocyanine-conjugated Au nanoparticles (NPs) significantly improve the interparticle-carrier-transport properties. The deposition of a solution of the Au NPs under ambient conditions results in an electroconductive metallic thin film without further post-treatment. Maximum conductivity reaches >6600 S cm?1 and the conductivity remains unchanged for at least 1 year under ambient conditions. The all-solution-processed organic field-effect transistor (OFET) fabricated under ambient conditions exhibits mobility values as high as 2 cm2 V?1 s?1, the value of which is comparable to OFET devices having vacuum-deposited Au electrodes.
en-copyright=
kn-copyright=

en-aut-name=KaneharaMasayuki
en-aut-sei=Kanehara
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakeyaJun
en-aut-sei=Takeya
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=UemuraTakafumi
en-aut-sei=Uemura
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurataHideyuki
en-aut-sei=Murata
en-aut-mei=Hideyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=TakimiyaKazuo
en-aut-sei=Takimiya
en-aut-mei=Kazuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=SekineHikaru
en-aut-sei=Sekine
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=TeranishiToshiharu
en-aut-sei=Teranishi
en-aut-mei=Toshiharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Department of Chemistry, Graduate School of Science, Osaka University

affil-num=3
en-affil=
kn-affil=Department of Chemistry, Graduate School of Science, Osaka University

affil-num=4
en-affil=
kn-affil=School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST)

affil-num=5
en-affil=
kn-affil=Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University

affil-num=6
en-affil=
kn-affil=Department of Chemistry, Graduate School of Pure and Applied Sciences, University of Tsukuba

affil-num=7
en-affil=
kn-affil=Department of Chemistry, Graduate School of Pure and Applied Sciences, University of Tsukuba
END

start-ver=1.4
cd-journal=joma
no-vol=3
cd-vols=
no-issue=2
article-no=
start-page=354
end-page=359
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.
en-copyright=
kn-copyright=

en-aut-name=WatanabeKoyo
en-aut-sei=Watanabe
en-aut-mei=Koyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KawataFukukazu
en-aut-sei=Kawata
en-aut-mei=Fukukazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=NagataKotaro
en-aut-sei=Nagata
en-aut-mei=Kotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NingJun
en-aut-sei=Ning
en-aut-mei=Jun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=KanoHiroshi
en-aut-sei=Kano
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Division of Information and Electronic Engineering, Graduate School of Engineering, Muroran Institute of Technology

affil-num=4
en-affil=
kn-affil=Division of Information and Electronic Engineering, Graduate School of Engineering, Muroran Institute of Technology

affil-num=5
en-affil=
kn-affil=Division of Engineering for Composite Functions, Graduate School of Engineering, Muroran Institute of Technology

affil-num=6
en-affil=
kn-affil=Division of Engineering for Composite Functions, Graduate School of Engineering, Muroran Institute of Technology
END

start-ver=1.4
cd-journal=joma
no-vol=27
cd-vols=
no-issue=1
article-no=
start-page=133
end-page=143
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=20120101
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Natural genetic variation in fluctuating asymmetry of wing shape in Drosophila melanogaster
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Fluctuating asymmetry (FA), defined as random deviation from perfect symmetry, has been used to assay the inability of individuals to buffer their developmental processes from environmental perturbations (i.e., developmental instability). In this study, we aimed to characterize the natural genetic variation in FA of wing shape in Drosophila melanogaster, collected from across the Japanese archipelago. We quantified wing shapes at whole wing and partial wing component levels and evaluated their mean and FA. We also estimated the heritability of the mean and FA of these traits. We found significant natural genetic variation in all the mean wing traits and in FA of one of the partial wing components. Heritability estimates for mean wing shapes were significant in two and four out of five wing traits in males and females, respectively. On the contrary, heritability estimates for FA were low and not significant. This is a novel study of natural genetic variation in FA of wing shape. Our findings suggest that partial wing components behave as distinct units of selection for FA, and local adaptation of the mechanisms to stabilize developmental processes occur in nature.
en-copyright=
kn-copyright=

en-aut-name=TsujinoMasahiro
en-aut-sei=Tsujino
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakahashiKazuo H.
en-aut-sei=Takahashi
en-aut-mei=Kazuo H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University
en-keyword=Animal model
kn-keyword=Animal model
en-keyword=Geometric morphometrics
kn-keyword=Geometric morphometrics
en-keyword=Heritability
kn-keyword=Heritability
en-keyword=Natural genetic variation
kn-keyword=Natural genetic variation
en-keyword=Wing shape
kn-keyword=Wing shape
END

start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=1
article-no=
start-page=109
end-page=115
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Screening of sperm velocity by fluid mechanical characteristics of a cyclo-olefin polymer microfluidic sperm-sorting device
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The microfluidic sperm-sorting (MFSS) device is a promising advancement for assisted reproductive technology. Previously, poly(dimethylsiloxiane) and quartz MFSS devices were developed and used for intracytoplasmic sperm injection. However, these disposable devices were not clinically suitable for assisted reproduction, so a cyclo-olefin polymer MFSS (COP-MFSS) device was developed. By micromachining, two microfluidic channels with different heights and widths (chip A: 0.3 x 0.5 mm; chip B: 0.1 x 0.6 mm) were prepared. Sorted sperm concentrations were similar in both microfluidic channels. Linear-velocity distribution using the microfluidic channel of chip B was higher than that of chip A. Using confocal fluorescence microscopy, it was found that the highest number of motile spermatozoa swam across the laminar flow at the bottom of the microfluidic channel. The time required to swim across the laminar flow was longer at the bottom and top of the microfluidic channels than in the middle because of the low fluid velocity. These results experimentally demonstrated that the width of microfluidic channels should be increased in the region of laminar flow from the semen inlet to the outlet for unsorted spermatozoa to selectively recover spermatozoa with high linear velocity.
en-copyright=
kn-copyright=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=TakenamiMami
en-aut-sei=Takenami
en-aut-mei=Mami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurodaYuka
en-aut-sei=Kuroda
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HyakutakeToru
en-aut-sei=Hyakutake
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YanaseShinichiro
en-aut-sei=Yanase
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Faculty of Engineering, Yokohama National University

affil-num=5
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=6
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=laminar flow
kn-keyword=laminar flow
en-keyword=linear velocity
kn-keyword=linear velocity
en-keyword=microfluidic sperm sorting
kn-keyword=microfluidic sperm sorting
en-keyword=motility
kn-keyword=motility
END

start-ver=1.4
cd-journal=joma
no-vol=2012
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=2012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Theoretical Study on the Detection of Tilted Lipid Bilayers Using Surface Plasmon Resonance Techniques
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Effective refractive indices detected using surface plasmon resonance techniques are calculated as a function of the tilt angle of lipid bilayers in a multilayered model. The changes in the effective refractive indices are derived from the shift of an excitation angle of surface plasmon. To obtain effective refractive index plots, we determined refractive index changes in the lipid bilayers with 3 and 5?nm thicknesses as a function of tilt angle and obtained a relationship between the effective refractive index and tilt angle. We also showed that the effective refractive index depended on the lipid bilayers thickness and anisotropic permittivities, which vary with interchain distance.
en-copyright=
kn-copyright=

en-aut-name=WatanabeKoyo
en-aut-sei=Watanabe
en-aut-mei=Koyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=10
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=201110
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Design of a variable-stiffness robotic hand using pneumatic soft rubber actuators
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In recent years, Japanese society has been ageing, engendering a labor shortage of young workers. Robots are therefore expected to be useful in performing tasks such as day-to-day support for elderly people. In particular, robots that are intended for use in the field of medical care and welfare are expected to be safe when operating in a human environment because they often come into contact with people. Furthermore, robots must perform various tasks such as regrasping, grasping of soft objects, and tasks using frictional force. Given these demands and circumstances, a tendon-driven robot hand with a stiffness changing finger has been developed. The finger surface stiffness can be altered by adjusting the input pressure depending on the task. Additionally, the coefficient of static friction can be altered by changing the surface stiffness merely by adjusting the input air pressure. This report describes the basic structure, driving mechanism, and basic properties of the proposed robot hand.
en-copyright=
kn-copyright=

en-aut-name=NagaseJun-ya
en-aut-sei=Nagase
en-aut-mei=Jun-ya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SatohToshiyuki
en-aut-sei=Satoh
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=SagaNorihiko
en-aut-sei=Saga
en-aut-mei=Norihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=School of Science and Technology, Kwansei Gakuin University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Faculty of Systems Science and Technology, Akita Prefectural University

affil-num=4
en-affil=
kn-affil=School of Science and Technology, Kwansei Gakuin University

affil-num=5
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=29
cd-vols=
no-issue=7
article-no=
start-page=619
end-page=625
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110915
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Design and Basic Characteristics of Pneumatic Rubber Tube Actuator for Colonoscope Insertion
kn-title=‘å’°“àŽ‹‹¾‘}“üŽx‰‡‚ð–ÚŽw‚µ‚½ƒ‰ƒo�[ƒ`ƒ…�[ƒuƒAƒNƒ`ƒ…ƒG�[ƒ^‚Ì�ÝŒv‚ÆŠî‘b‹ì“®“Á�«
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Colonoscopy is an important medical action to detect disorders like colon cancer. However generally it is difficult to insert a scope into the colon, because the colon is flexible and complex shape. This study aims at development of an actuator which can add propelling ability to a colonoscope. We focus on rubber pneumatic actuators, because advantages of them, for example, high compliance, low cost, water proof, and so on, agree with the required properties of medical devices. In this paper, we design a novel rubber actuator consisting of three air chambers by nonlinear FEM (finite element method) and fabricate the actuator employing the design by extrusion molding method. Basic characteristics of the actuator are clarified by a motion capture system, and functional motion leading assisting colonoscope insertion is observed. Actually using dummy endoscope, transportation ability by the actuators is confirmed experimentally and insertion experiments into a large intestine phantom are conducted.
en-copyright=
kn-copyright=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=˜eŒ³�Cˆê
kn-aut-sei=˜eŒ³
kn-aut-mei=�Cˆê
aut-affil-num=1
ORCID=

en-aut-name=OzakiKen
en-aut-sei=Ozaki
en-aut-mei=Ken
kn-aut-name=”ö�茒
kn-aut-sei=”ö�è
kn-aut-mei=Œ’
aut-affil-num=2
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=—é�X�Nˆê
kn-aut-sei=—é�X
kn-aut-mei=�Nˆê
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=‰ªŽR‘åŠw

affil-num=2
en-affil=
kn-affil=‰ªŽR‘åŠw

affil-num=3
en-affil=
kn-affil=‰ªŽR‘åŠw
en-keyword=Soft Actuator
kn-keyword=Soft Actuator
en-keyword=Colonoscope Robot
kn-keyword=Colonoscope Robot
en-keyword=Soft Mechanism
kn-keyword=Soft Mechanism
END

start-ver=1.4
cd-journal=joma
no-vol=46
cd-vols=
no-issue=9
article-no=
start-page=517
end-page=523
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110905
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Development of Balloon Type Tendon-driven Robot Hand with Stiffness Change Finger
kn-title=‹ó‹Cˆ³�„�«‰Â•ÏƒtƒBƒ“ƒK‚ð—L‚·‚éƒoƒ‹�[ƒ“Œ^äF‹ì“®ƒ�ƒ{ƒbƒgƒnƒ“ƒh‚ÌŠJ”­
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=NagaseJun-ya
en-aut-sei=Nagase
en-aut-mei=Jun-ya
kn-aut-name=‰i�£�ƒ–ç
kn-aut-sei=‰i�£
kn-aut-mei=�ƒ–ç
aut-affil-num=1
ORCID=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=˜eŒ³�Cˆê
kn-aut-sei=˜eŒ³
kn-aut-mei=�Cˆê
aut-affil-num=2
ORCID=

en-aut-name=SagaNorihiko
en-aut-sei=Saga
en-aut-mei=Norihiko
kn-aut-name=�µ‰ã�é•F
kn-aut-sei=�µ‰ã
kn-aut-mei=�é•F
aut-affil-num=3
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=—é�X�Nˆê
kn-aut-sei=—é�X
kn-aut-mei=�Nˆê
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=ŠÖ�¼Šw‰@‘åŠw

affil-num=2
en-affil=
kn-affil=‰ªŽR‘åŠw

affil-num=3
en-affil=
kn-affil=ŠÖ�¼Šw‰@‘åŠw

affil-num=4
en-affil=
kn-affil=‰ªŽR‘åŠw
en-keyword=stiffness change device
kn-keyword=stiffness change device
en-keyword=flexible finger
kn-keyword=flexible finger
en-keyword=pneumatic balloon
kn-keyword=pneumatic balloon
en-keyword=robot hand
kn-keyword=robot hand
en-keyword=tendon-driven
kn-keyword=tendon-driven
END

start-ver=1.4
cd-journal=joma
no-vol=102
cd-vols=
no-issue=4
article-no=
start-page=448
end-page=457
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=201108
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Genome-wide deficiency mapping of the regions responsible for temporal canalization of the developmental processes of Drosophila melanogaster
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Developmental processes of organisms are programed to proceed in a finely regulated manner and finish within a certain period of time depending on the ambient environmental conditions. Therefore, variation in the developmental period under controlled genetic and environmental conditions indicates innate instability of the developmental process. In this study, we aimed to determine whether a molecular machinery exists that regulates the canalization of the developmental period and, if so, to test whether the same mechanism also stabilizes a morphological trait. To search for regions that influence the instability of the developmental period, we conducted genome-wide deficiency mapping with 441 isogenic deficiency strains covering 65.5% of the Drosophila melanogaster genome. We found that 11 independent deficiencies significantly increased the instability of the developmental period and 5 of these also significantly increased the fluctuating asymmetry of wing shape although there was no significant correlation between the instabilities of developmental period and wing shape in general. These results suggest that canalization processes of the developmental period and morphological traits are at least partially independent. Our findings emphasize the potential importance of temporal variation in development as an indicator of developmental stability and canalization and provide a novel perspective for understanding the regulation of phenotypic variability.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKazuo H.
en-aut-sei=Takahashi
en-aut-mei=Kazuo H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkadaYasukazu
en-aut-sei=Okada
en-aut-mei=Yasukazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TeramuraKouhei
en-aut-sei=Teramura
en-aut-mei=Kouhei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University
en-keyword=developmental period
kn-keyword=developmental period
en-keyword=developmental stability
kn-keyword=developmental stability
en-keyword=Drosophila melanogaster
kn-keyword=Drosophila melanogaster
en-keyword=wing shape
kn-keyword=wing shape
END

start-ver=1.4
cd-journal=joma
no-vol=12
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110622
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Genome-wide deficiency screen for the genomic regions responsible for heat resistance in Drosophila melanogaster
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: Temperature adaptation is one of the most important determinants of distribution and population size of organisms in nature. Recently, quantitative trait loci (QTL) mapping and gene expression profiling approaches have been used for detecting candidate genes for heat resistance. However, the resolution of QTL mapping is not high enough to examine the individual effects of various genes in each QTL. Heat stress-responsive genes, characterized by gene expression profiling studies, are not necessarily responsible for heat resistance. Some of these genes may be regulated in association with the heat stress response of other genes. 

Results: To evaluate which heat-responsive genes are potential candidates for heat resistance with higher resolution than previous QTL mapping studies, we performed genome-wide deficiency screen for QTL for heat resistance. We screened 439 isogenic deficiency strains from the DrosDel project, covering 65.6% of the Drosophila melanogaster genome in order to map QTL for thermal resistance. As a result, we found 19 QTL for heat resistance, including 3 novel QTL outside the QTL found in previous studies. 

Conclusion: The QTL found in this study encompassed 19 heat-responsive genes found in the previous gene expression profiling studies, suggesting that they were strong candidates for heat resistance. This result provides new insights into the genetic architecture of heat resistance. It also emphasizes the advantages of genome-wide deficiency screen using isogenic deficiency libraries.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKazuo H.
en-aut-sei=Takahashi
en-aut-mei=Kazuo H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=OkadaYasukazu
en-aut-sei=Okada
en-aut-mei=Yasukazu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TeramuraKouhei
en-aut-sei=Teramura
en-aut-mei=Kouhei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Environmental Science, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=6
cd-vols=
no-issue=4
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=20110425
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Environmental Stress-Dependent Effects of Deletions Encompassing Hsp70Ba on Canalization and Quantitative Trait Asymmetry in Drosophila melanogaster
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hsp70 genes may influence the expression of wing abnormalities in Drosophila melanogaster but their effects on variability in quantitative characters and developmental instability are unclear. In this study, we focused on one of the six Hsp70 genes, Hsp70Ba, and investigated its effects on within-and among-individual variability in orbital bristle number, sternopleural bristle number, wing size and wing shape under different environmental conditions. To do this, we studied a newly constructed deletion, Df(3R)ED5579, which encompasses Hsp70Ba and nine non-Hsp genes, in the heterozygous condition and another, Hsp70Ba(304), which deletes only Hsp70Ba, in the homozygous condition. We found no significant effect of both deletions on within-individual variation quantified by fluctuating asymmetry (FA) of morphological traits. On the other hand, the Hsp70Ba(304)/Hsp70Ba(304) genotype significantly increased among-individual variation quantified by coefficient of variation (CV) of bristle number and wing size in female, while the Df(3R)ED5579 heterozygote showed no significant effect. The expression level of Hsp70Ba in the deletion heterozygote was 6 to 20 times higher than in control homozygotes, suggesting that the overexpression of Hsp70Ba did not influence developmental stability or canalization significantly. These findings suggest that the absence of expression of Hsp70Ba increases CV of some morphological traits and that HSP70Ba may buffer against environmental perturbations on some quantitative traits.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKazuo H.
en-aut-sei=Takahashi
en-aut-mei=Kazuo H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=DabornPhillip J.
en-aut-sei=Daborn
en-aut-mei=Phillip J.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=HoffmannAry A.
en-aut-sei=Hoffmann
en-aut-mei=Ary A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Takano-ShimizuToshiyuki
en-aut-sei=Takano-Shimizu
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Population Genetics, National Institute of Genetics

affil-num=2
en-affil=
kn-affil=Centre for Environmental Stress and Adaptation Research and Department of Genetics, The University of Melbourne

affil-num=3
en-affil=
kn-affil=Centre for Environmental Stress and Adaptation Research and Department of Genetics, The University of Melbourne

affil-num=4
en-affil=
kn-affil=Department of Population Genetics, National Institute of Genetics
END

start-ver=1.4
cd-journal=joma
no-vol=25
cd-vols=
no-issue=9-10
article-no=
start-page=1311
end-page=1330
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=2011
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Miniature Pneumatic Curling Rubber Actuator Generating Bidirectional Motion with One Air-Supply Tube
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Soft actuators driven by pneumatic pressure are promising actuators for mechanical systems in medical, biological, agriculture, welfare fields and so on, because they can ensure high safety for fragile objects from their low mechanical impedance. In this study, a new rubber pneumatic actuator made from silicone rubber was developed. Composed of one chamber and one air-supply tube, it can generate curling motion in two directions by using positive and negative pneumatic pressure. The rubber actuator, for generating bidirectional motion, was designed to achieve an efficient shape by nonlinear finite element method analysis, and was fabricated by a molding and rubber bonding process using excimer light. The fabricated actuator was able to generate curling motion in two directions successfully. The displacement and force characteristics of the actuator were measured by using a motion capture system and a load cell. As an example application of the actuator, a robotic soft hand with three actuators was constructed and its effectiveness was confirmed by experiments.
en-copyright=
kn-copyright=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=OguraKeiko
en-aut-sei=Ogura
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
en-keyword=Soft actuator
kn-keyword=Soft actuator
en-keyword=pneumatic actuator
kn-keyword=pneumatic actuator
en-keyword=bidirectional motion
kn-keyword=bidirectional motion
en-keyword=nonlinear analysis
kn-keyword=nonlinear analysis
END

start-ver=1.4
cd-journal=joma
no-vol=164
cd-vols=
no-issue=1-2
article-no=
start-page=88
end-page=94
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Multiplex pneumatic control method for multi-drive system
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Pneumatic actuators have several advantages such as light weight safety low cost and high compliance However many pneumatic actuators have complicated systems that include a compressor air tubes and pneumatic valves with electrical wires This research proposes a new control method for a multiplex pneumatic transmission constructed with special resonant valves and air tubes with a control system driven by air vibration in air tubes without electrical wires The control is simplified and effective for pneumatic systems having many degrees of freedom In this paper the development of a primitive model of the resonant valve and a prototype valve is described In addition two control methods which are a superimposing method and a time-sharing method are shown and the independent driving of four actuators is realized by using one of the control methods with air tubes only.
en-copyright=
kn-copyright=

en-aut-name=NishiokaYasutaka
en-aut-sei=Nishioka
en-aut-mei=Yasutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KandaTakefumi
en-aut-sei=Kanda
en-aut-mei=Takefumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University
en-keyword=Pneumatic
kn-keyword=Pneumatic
en-keyword=Valve
kn-keyword=Valve
en-keyword=Actuator
kn-keyword=Actuator
en-keyword=Multi-DOF
kn-keyword=Multi-DOF
en-keyword=Resonant
kn-keyword=Resonant
en-keyword=Mechatronics
kn-keyword=Mechatronics
END

start-ver=1.4
cd-journal=joma
no-vol=57
cd-vols=
no-issue=1
article-no=
start-page=163
end-page=167
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20101012
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Hydrophobic Silicone Elastomer Chamber for Recording Trajectories of Motile Porcine Sperms without Adsorption
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.
en-copyright=
kn-copyright=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KurodaYuka
en-aut-sei=Kuroda
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YamashitaKeisuke
en-aut-sei=Yamashita
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Department of Animal Science, Faculty of Agriculture, Okayama University

affil-num=4
en-affil=
kn-affil=Department of Animal Science, Faculty of Agriculture, Okayama University
en-keyword=Adsorption
kn-keyword=Adsorption
en-keyword=Porcine sperm motility
kn-keyword=Porcine sperm motility
en-keyword=Silicone elastomer
kn-keyword=Silicone elastomer
en-keyword=Trajectories
kn-keyword=Trajectories
END

start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=5
article-no=
start-page=552
end-page=557
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P<0.05). These observations indicate that tilting culture during IVM and IVC in chemically defined media improves the quality of blastocyst, as determined by the number of cells per blastocyst, without any effects on penetrability and developmental competence.
en-copyright=
kn-copyright=

en-aut-name=KoikeTakayuki
en-aut-sei=Koike
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Department of Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University
en-keyword=Inclining device
kn-keyword=Inclining device
en-keyword=In vitro culture
kn-keyword=In vitro culture
en-keyword=In vitro fertilization
kn-keyword=In vitro fertilization
en-keyword=Oocytes
kn-keyword=Oocytes
en-keyword=Pig
kn-keyword=Pig
END

start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100916
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Effects of small Hsp genes on developmental stability and microenvironmental canalization
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background:
Progression of development has to be insulated from the damaging impacts of environmental and genetic perturbations to produce highly predictable phenotypes. Molecular chaperones, such as the heat shock proteins (HSPs), are known to buffer various environmental stresses, and are deeply involved in protein homeostasis. These characteristics of HSPs imply that they might affect developmental buffering and canalization.

Results:
We examined the role of nine Hsp genes using the GAL4/UAS-RNAi system on phenotypic variation of various morphological traits in Drosophila melanogaster. The stability of bristle number, wing size and wing shape was characterized through fluctuating asymmetry (FA) and the coefficient of variation (CV), or among-individual variation. Progeny of the GAL4/Hsp-RNAi crosses tended to have reduced trait means for both wing size and wing shape. Transcriptional knockdown of Hsp67Bc and Hsp22 significantly increased FA of bristle number, while knockdown of Hsp67Ba significantly increased FA and among-individual variation of wing shape but only in males. Suppression of Hsp67Bb expression significantly increased among-individual variation of bristle number. The knockdown of gene expression was confirmed for Hsp67Ba, Hsp67Bc, Hsp22, and Hsp67Bb. Correlation between FA and CV or among-individual variation of each trait is weak and not significant except for the case of male wing shape.

Conclusion:
Four small Hsp genes (Hsp22, Hsp67Ba, Hsp67Bb and Hsp67Bc) showed involvement in the processes of morphogenesis and developmental stability. Due to possible different functions in terms of developmental buffering of these small Hsps, phenotypic stability of an organism is probably maintained by multiple mechanisms triggered by different environmental and genetic stresses on different traits. This novel finding may lead to a better understanding of non-Hsp90 molecular mechanisms controlling variability in morphological traits.
en-copyright=
kn-copyright=

en-aut-name=TakahashiKazuo H.
en-aut-sei=Takahashi
en-aut-mei=Kazuo H.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=RakoLea
en-aut-sei=Rako
en-aut-mei=Lea
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=Takano-ShimizuToshiyuki
en-aut-sei=Takano-Shimizu
en-aut-mei=Toshiyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HoffmannAry A.
en-aut-sei=Hoffmann
en-aut-mei=Ary A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=LeeSiu F.
en-aut-sei=Lee
en-aut-mei=Siu F.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio21 Institute, The University of Melbourne

affil-num=3
en-affil=
kn-affil=Department of Population Genetics, National Institute of Genetics

affil-num=4
en-affil=
kn-affil=Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio21 Institute, The University of Melbourne

affil-num=5
en-affil=
kn-affil=Centre for Environmental Stress and Adaptation Research, Department of Genetics, Bio21 Institute, The University of Melbourne
END

start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=5
article-no=
start-page=863
end-page=870
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100915
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Application of a microfluidic sperm sorter to the in-vitro fertilization of porcine oocytes reduced the incidence of polyspermic penetration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The objective of this study was to use a microfluidic sperm sorter (MFSS), designed to isolate motile human spermatozoa with laminar flows (no centrifugation), for porcine IVF. Boar spermatozoa were diluted at 1 x 10(8) with a diluent containing 20% seminal fluid and flowed with modified TCM-199 (mM199, with 5 mM caffeine) to introduce motile sperm into the exit chamber for IVF. In Experiment 1, after flowing for 5 min, sperm concentration varied significantly among specific sites within the MFSS collecting chamber (range, 0.8 +/- 0.5 x 10(4) to 575.0 +/- 56.3 x 10(4) cells/mL; mean +/- SEM). In Experiment 2, when porcine IVM oocytes were placed at three locations in the MFSS exit chamber (where only motile spermatozoa accumulated) and subsequently cultured in caffeine-free mM199 for 8 h, sperm penetration rate was not significantly different among places (86.1 +/- 10.5 to 100%), but the monospermic penetration rate was lower (P < 0.05) in oocytes 3.5 mm from the exit position (12.5 +/- 4.8%) than those at 7.5 mm (53.1 +/- 6.0%) or further (41.9 +/- 2.8%) from the exit. In Experiment 3, the normal fertilization index (ratio of monospermic oocytes to number of oocytes examined) 8 h after insemination was higher (P < 0.05) in the MFSS-IVF system (0.375 +/- 0.040) than both standard IVF and transient IVF (0.222 +/- 0.028 and 0.189 +/- 0.027, respectively, with co-culture for 8 h and for 5 min). Developmental competence of fertilized oocytes (blastocyst formation) was higher (P < 0.05) in the MFSS-IVF system (40.9 +/- 2.3%) than in either standard or transient IVF (22.6 +/- 1.4 and 33.7 +/- 3.5%). In conclusion, brief co-culture of porcine oocytes with spermatozoa gradually accumulated in the MFSS chamber improved the efficiency of producing monospermic fertilized embryos and blastocysts. Furthermore, efficiencies were significantly affected by oocyte location within the chamber.
en-copyright=
kn-copyright=

en-aut-name=SanoHikaru
en-aut-sei=Sano
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Animal Science, Graduate school of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Department of Animal Science, Graduate school of Natural Science and Technology, Okayama University
en-keyword=Oocytes
kn-keyword=Oocytes
en-keyword=Polyspermy
kn-keyword=Polyspermy
en-keyword=IVF
kn-keyword=IVF
en-keyword=Sperm sorter
kn-keyword=Sperm sorter
en-keyword=Pig
kn-keyword=Pig
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3
article-no=
start-page=1135
end-page=1137
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201008
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Blastocyst quality scoring based on morphologic grading correlates with cell number
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Blastocyst quality score (BQS), first reported by Rehman et al., is a numerical blastocyst-morphology grading system based on the criteria established by Gardner and Schoolcraft. We demonstrate a positive correlation between the calculated BQS score and cell number by staining thawed human embryos and suggest that BQS can be applied to evaluate culture systems clinically.
en-copyright=
kn-copyright=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HayashiNobuyoshi
en-aut-sei=Hayashi
en-aut-mei=Nobuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TakiueChisato
en-aut-sei=Takiue
en-aut-mei=Chisato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=HirataRei
en-aut-sei=Hirata
en-aut-mei=Rei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HabaraToshihiro
en-aut-sei=Habara
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=3
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=4
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=5
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=6
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
END

start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=3
article-no=
start-page=358
end-page=364
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201003
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Improved development of mouse and human embryos using a tilting embryo culture system
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Mammalian embryos experience not only hormonal but also mechanical stimuli, such as shear stress, compression and friction force in the Fallopian tube before nidation. In order to apply mechanical stimuli to embryos in a conventional IVF culture system, the tilting embryo culture system (TECS) was developed. The observed embryo images from the TECS suggest that the velocities and shear stresses of TECS embryos are similar to those experienced in the oviduct. Use of TECS enhanced the development rate to the blastocyst stage and significantly increased the cell number of mouse blastocysts (P < 0.05). Although not statistically significant, human thawed embryos showed slight improvement in development to the blastocyst stage following culture in TECS compared with static controls. Rates of blastocyst formation following culture in TECS were significantly improved in low-quality embryos and those embryos cultured under suboptimal conditions (P < 0.05). The TECS is proposed as a promising approach to improve embryo development and blastocyst formation by exposing embryos to mechanical stimuli similar to those in the Fallopian tube.
en-copyright=
kn-copyright=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HayashiNobuyoshi
en-aut-sei=Hayashi
en-aut-mei=Nobuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KurodaYuka
en-aut-sei=Kuroda
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=TakiueChisato
en-aut-sei=Takiue
en-aut-mei=Chisato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=HirataRei
en-aut-sei=Hirata
en-aut-mei=Rei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakenamiMami
en-aut-sei=Takenami
en-aut-mei=Mami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=AoiYoko
en-aut-sei=Aoi
en-aut-mei=Yoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=YoshiokaNanako
en-aut-sei=Yoshioka
en-aut-mei=Nanako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=HabaraToshihiro
en-aut-sei=Habara
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

en-aut-name=MukaidaTetsunori
en-aut-sei=Mukaida
en-aut-mei=Tetsunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=

affil-num=1
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=2
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=3
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=4
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=5
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=6
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=7
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=8
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=9
en-affil=
kn-affil=Okayama Couples Clinic

affil-num=10
en-affil=
kn-affil=Hiroshima HART Clinic

affil-num=11
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=blastocyst
kn-keyword=blastocyst
en-keyword=embryo development
kn-keyword=embryo development
en-keyword=mechanical stimuli
kn-keyword=mechanical stimuli
en-keyword=shear stress
kn-keyword=shear stress
en-keyword=tilting embryo culture system
kn-keyword=tilting embryo culture system
END

start-ver=1.4
cd-journal=joma
no-vol=24
cd-vols=
no-issue=10
article-no=
start-page=1503
end-page=1528
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=2010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Development of an Intelligent Chair Tool System Applying New Intelligent Pneumatic Actuators
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This paper develops an Intelligent Chair Tool (ICT) - a new chair-type human and machine interaction seating system powered by 36 intelligent pneumatic actuators. This tool can be used to facilitate investigation of chair shapes from spring and damping effects of seating and backrest surfaces. Each actuator used consists of five extensive elements of encoder, laser strip rod, pressure sensor, valves and PSoC microcontroller incorporated in a single device. By using the ICT, different shapes, spring and damping characteristics can be obtained to aid the design of chairs from the control inputs, i.e., position x, stiffness k(s) and viscous coefficient c. Several control algorithms are presented to realize the communication and control system, and to obtain all data in real-time. The control methodology presented contains an inner force loop and an outer position loop implemented using a unified control system. The specification, development design and experimental evaluation of the ICT control system and actuator used are presented and discussed.
en-copyright=
kn-copyright=

en-aut-name=FaudziAhmad 'Athif Mohd
en-aut-sei=Faudzi
en-aut-mei=Ahmad 'Athif Mohd
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SuzumoriKoichi
en-aut-sei=Suzumori
en-aut-mei=Koichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WakimotoShuichi
en-aut-sei=Wakimoto
en-aut-mei=Shuichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University
en-keyword=Intelligent Chair Tool
kn-keyword=Intelligent Chair Tool
en-keyword=seating system
kn-keyword=seating system
en-keyword=intelligent pneumatic actuator
kn-keyword=intelligent pneumatic actuator
en-keyword=physical human machine interaction
kn-keyword=physical human machine interaction
en-keyword=control application in mechatronics
kn-keyword=control application in mechatronics
END

start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=19
article-no=
start-page=4205
end-page=4215
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=20091001
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=mTrs130 Is a Component of a Mammalian TRAPPII Complex, a Rab1 GEF That Binds to COPI-coated Vesicles
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to ƒÁ1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.
en-copyright=
kn-copyright=

en-aut-name=YamasakiAkinori
en-aut-sei=Yamasaki
en-aut-mei=Akinori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MenonShekar
en-aut-sei=Menon
en-aut-mei=Shekar
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YuSidney
en-aut-sei=Yu
en-aut-mei=Sidney
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=BarrowmanJemima
en-aut-sei=Barrowman
en-aut-mei=Jemima
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MeerlooTimo
en-aut-sei=Meerloo
en-aut-mei=Timo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=OorschotViola
en-aut-sei=Oorschot
en-aut-mei=Viola
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=KlumpermanJudith
en-aut-sei=Klumperman
en-aut-mei=Judith
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=SatohAyano
en-aut-sei=Satoh
en-aut-mei=Ayano
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=Ferro-NovickSusan
en-aut-sei=Ferro-Novick
en-aut-mei=Susan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine

affil-num=2
en-affil=
kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine

affil-num=3
en-affil=
kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine

affil-num=4
en-affil=
kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine

affil-num=5
en-affil=
kn-affil=Department of Cellular and Molecular Medicine, University of California at San Diego

affil-num=6
en-affil=
kn-affil=Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht

affil-num=7
en-affil=
kn-affil=Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht

affil-num=8
en-affil=
kn-affil=Department of Cell Biology, Yale University School of Medicine

affil-num=9
en-affil=
kn-affil=Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine
END

start-ver=1.4
cd-journal=joma
no-vol=117
cd-vols=
no-issue=1364
article-no=
start-page=411
end-page=414
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=20090401
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The preparation and characterization of TiO2/ZrO2 composites doped with PMA/PWA
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A new class of glass composite membranes of various compositions was prepared via a sol-gel technique. The membranes, consisting of (phophotungsticacid/phosphomolybdicacid) PWA/PMA-P2O5-SiO2 mixed with either ZrO2 or TiO2 displayed varying properties depending on their composition and mode of fabrication. The structure and property of the obtained glass ceramic composite membranes were investigated by FTIR, TG/DTA and textural analysis. The results included good mechanical, textural and thermal properties and the materials were deemed to be suitable electrolytes for fuel cell applications.
en-copyright=
kn-copyright=

en-aut-name=UmaThanganathan
en-aut-sei=Uma
en-aut-mei=Thanganathan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=NogamiMasayuki
en-aut-sei=Nogami
en-aut-mei=Masayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Materials Science and Engineering, Nagoya Institute of Technology

affil-num=2
en-affil=
kn-affil=Department of Materials Science and Engineering, Nagoya Institute of Technology
en-keyword=Glass
kn-keyword=Glass
en-keyword=Heteropolyacids
kn-keyword=Heteropolyacids
en-keyword=FTIR
kn-keyword=FTIR
en-keyword=Thermal
kn-keyword=Thermal
en-keyword=Textural
kn-keyword=Textural
END

start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=1
article-no=
start-page=25
end-page=33
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Application of a numerical simulation to improve the separation efficiency of a sperm sorter
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This paper describes a study in which numerical simulations were applied to improve the separation efficiency of a microfluidic-based sperm sorter. Initially, the motion of 31 sperm were modeled as a sinusoidal wave. The modeled sperm were expected to move while vibrating in the fluid within the microchannel. In this analysis, the number of sperm extracted at the outlet channel and the rate of movement of the highly motile sperm were obtained for a wide range of flow velocities within the microchannel. By varying the channel height, and the width and the position of the sperm-inlet channel, we confirmed that the separation efficiency was highly dependent on the fluid velocity within the channel. These results will be valuable for improving the device configuration, and might help to realize further improvements in efficiency in the future.
en-copyright=
kn-copyright=

en-aut-name=HyakutakeToru
en-aut-sei=Hyakutake
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=HashimotoYuki
en-aut-sei=Hashimoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=YanaseShinichiro
en-aut-sei=Yanase
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=4
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

affil-num=5
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=Human reproduction
kn-keyword=Human reproduction
en-keyword=Microfluid
kn-keyword=Microfluid
en-keyword=Numerical simulation
kn-keyword=Numerical simulation
en-keyword=Separation efficiency
kn-keyword=Separation efficiency
en-keyword=Sperm sorter
kn-keyword=Sperm sorter
END

start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=22
article-no=
start-page=3306
end-page=3309
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=2009
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Fabricating small-scale, curved, polymeric structures with convex and concave menisci through interfacial free energy equilibrium
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Polymeric curved structures are widely used in imaging systems including optical fibers and microfluidic channels. Here, we demonstrate that small-scale, poly(dimethylsiloxane) (PDMS)-based, curved structures can be fabricated through controlling interfacial free energy equilibrium. Resultant structures have a smooth, symmetric, curved surface, and may be convex or concave in form based on surface tension balance. Their curvatures are controlled by surface characteristics (i.e., hydrophobicity and hydrophilicity) of the molds and semi-liquid PDMS. In addition, these structures are shown to be biocompatible for cell culture. Our system provides a simple, efficient and economical method for generating integrateable optical components without costly fabrication facilities.
en-copyright=
kn-copyright=

en-aut-name=ChengChao-Min
en-aut-sei=Cheng
en-aut-mei=Chao-Min
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=WangI-Jan
en-aut-sei=Wang
en-aut-mei=I-Jan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=KurodaYuka
en-aut-sei=Kuroda
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=LeDucPhilip R.
en-aut-sei=LeDuc
en-aut-mei=Philip R.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

affil-num=1
en-affil=
kn-affil=Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University

affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=3
en-affil=
kn-affil=Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University

affil-num=4
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University

affil-num=5
en-affil=
kn-affil=Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University

affil-num=6
en-affil=
kn-affil=Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
END