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ID 48703
FullText URL
Author
Kawakami, Keisuke
Koike, Hiroyuki
Abstract
This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
Keywords
Photosystem II
Oxygen evolution
Crystallization
Membrane proteins
Ion-exchange chromatography
Published Date
2011
Publication Title
Methods in Molecular Biology
Volume
volume684
Publisher
Humana Press
Start Page
41
End Page
51
ISSN
1064-3745
NCID
AA11067778
Content Type
Journal Article
Project
Research Center of New Functional Materials for Energy Production, Storage, and Transport
Official Url
http://www.springerlink.com/content/t782187342578257/
language
英語
Copyright Holders
© Springer Science+Business Media, LLC
File Version
author
Refereed
True
DOI
PubMed ID