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ID 60135
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Author
Nagaki, K.
Yamaji, N. Institute of Plant Science and Resources, Okayama University
Abstract
Information about the positioning of individual loci in the nucleus and the status of epigenetic modifications at these loci in each cell contained in plant tissue increases our understanding of how cells in a tissue coordinate gene expression. To obtain such information, a less damaging method of visualizing DNA in tissue that can be used with immunohistochemistry is required. Recently, a less damaging DNA visualization method using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/associated caspase 9) system, named RNA-guided endonuclease-in situ labeling (RGEN-ISL), was reported. This system made it possible to visualize a target DNA locus in a nucleus fixed on a glass slide with a set of simple operations, but it could not be applied to cells in plant tissues. In this work, we have developed a modified RGEN-ISL method with decrosslinking that made it possible to simultaneously detect the DNA loci and immunohistochemistry signals, including histone modification, in various types of plant tissues and species.
Keywords
Centromere
CRISPR/Cas9
epigenetic modifications
immunohistochemistry
in situ DNA visualization
RNA-guided endonuclease-in situ labeling (RGEN-ISL)
telomere
Published Date
2019-11-30
Publication Title
Journal of Experimental Botany
Volume
volume71
Issue
issue6
Publisher
Oxford University Press
Start Page
1792
End Page
1800
ISSN
0022-0957
NCID
AA00697526
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2019.
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isVersionOf https://doi.org/10.1093/jxb/erz534
License
http://creativecommons.org/licenses/by/4.0/