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Nomura, Hayato Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Suganuma, Mutsumi Department of Dermatology, Nagoya University Graduate School of Medicine
Takeichi, Takuya Department of Dermatology, Nagoya University Graduate School of Medicine
Kono, Michihiro Department of Dermatology and Plastic Surgery, Akita University Graduate School of Medicine
Isokane, Yuki Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Sunagawa, Ko Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Kobashi, Mina Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Sugihara, Satoru Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Kajita, Ai Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Miyake, Tomoko Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science ORCID Kaken ID
Hirai, Yoji Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science
Yamasaki, Osamu Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science Kaken ID publons researchmap
Akiyama, Masashi Department of Dermatology, Nagoya University Graduate School of Medicine
Morizane, Shin Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science ORCID Kaken ID publons researchmap
Abstract
The serine proteases kallikrein-related peptidase (KLK) 5 and KLK7 cleave cell adhesion molecules in the epidermis. Aberrant epidermal serine protease activity is thought to play an important role in the pathogenesis of atopic dermatitis (AD). We collected the stratum corneum (SC) from healthy individuals (n = 46) and AD patients (n = 63) by tape stripping and then measuring the trypsin- and chymotrypsin-like serine protease activity. We also analyzed the p.D386N and p.E420K of SPINK5 variants and loss-of-function mutations of FLG in the AD patients. The serine protease activity in the SC was increased not only in AD lesions but also in non-lesions of AD patients. We found, generally, that there was a positive correlation between the serine protease activity in the SC and the total serum immunoglobulin E (IgE) levels, serum thymus and activation-regulated chemokine (TARC) levels, and peripheral blood eosinophil counts. Moreover, the p.D386N or p.E420K in SPINK5 and FLG mutations were not significantly associated with the SC's serine protease activity. Epidermal serine protease activity was increased even in non-lesions of AD patients. Such activity was found to correlate with a number of biomarkers of AD. Further investigations of serine proteases might provide new treatments and prophylaxis for AD.
Keywords
atopic dermatitis
serine proteases
kallikrein-related peptidases
epidermal barrier dysfunction
lympho-epithelial Kazal-type-related inhibitor (LEKTI)
SPINK5
filaggrin
Published Date
2020-01-30
Publication Title
International Journal of Molecular Sciences
Volume
volume21
Issue
issue3
Publisher
MDPI
Start Page
913
ISSN
1422-0067
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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© 2020 by the authors.
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isVersionOf https://doi.org/10.3390/ijms21030913
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http://creativecommons.org/licenses/by/4.0/