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Abdelsalam, Sobhy S. H. Graduate School of Environmental and Life Science, Okayama University
Kouzai, Yusuke Bioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science
Watanabe, Megumi Graduate School of Environmental and Life Science, Okayama University
Inoue, Komaki Bioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science
Matsui, Hidenori Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life Science, Okayama University Kaken ID publons researchmap
Ichinose, Yuki Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Tsuge, Seiji Graduate School of Agriculture, Kyoto Prefectural University
Mochida, Keiichi Institute for Plant Science and Resources (IPSR), Okayama University ORCID Kaken ID publons researchmap
Noutoshi, Yoshiteru Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Abstract
Rhizoctonia solani is a necrotrophic phytopathogen belonging to basidiomycetes. It causes rice sheath blight which inflicts serious damage in rice production. The infection strategy of this pathogen remains unclear. We previously demonstrated that salicylic acid-induced immunity could block R. solani AG-1 IA infection in both rice and Brachypodium distachyon. R. solani may undergo biotrophic process using effector proteins to suppress host immunity before necrotrophic stage. To identify pathogen genes expressed at the early infection process, here we developed an inoculation method using B. distachyon which enables to sample an increased amount of semi-synchronous infection hyphae. Sixty-one R. solani secretory effector-like protein genes (RsSEPGs) were identified using in silico approach with the publicly available gene annotation of R. solani AG-1 IA genome and our RNA-sequencing results obtained from hyphae grown on agar medium. Expression of RsSEPGs was analyzed at 6, 10, 16, 24, and 32 h after inoculation by a quantitative reverse transcription-polymerase chain reaction and 52 genes could be detected at least on a single time point tested. Their expressions showed phase-specific patterns which were classified into 6 clusters. The 23 RsSEPGs in the cluster 1-3 and 29 RsSEPGs in the cluster 4-6 are expected to be involved in biotrophic and necrotrophic interactions, respectively.
Keywords
Fungi
Microbiology
Pathogens
Plant immunity
Plant sciences
Transcription
Published Date
2020-09-10
Publication Title
Scientific Reports
Volume
volume10
Issue
issue1
Publisher
Nature Research
Start Page
14889
ISSN
2045-2322
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2020
File Version
publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1038/s41598-020-71968-x
License
http://creat iveco mmons .org/licen ses/by/4.0/
Funder Name
Japan Science and Technology Agency
助成番号
18H02206