start-ver=1.4 cd-journal=joma no-vol=28 cd-vols= no-issue=6 article-no= start-page=673 end-page=681 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=201411 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Endothelin as a local regulating factor in the bovine oviduct en-subtitle= kn-subtitle= en-abstract= kn-abstract= Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17β increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells. en-copyright= kn-copyright= en-aut-name=YamamotoYuki en-aut-sei=Yamamoto en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KohkaMisa en-aut-sei=Kohka en-aut-mei=Misa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiYoshihiko en-aut-sei=Kobayashi en-aut-mei=Yoshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Woclawek-PotockaIzabela en-aut-sei=Woclawek-Potocka en-aut-mei=Izabela kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkudaKiyoshi en-aut-sei=Okuda en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences kn-affil= affil-num=5 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=endothelin converting enzyme kn-keyword=endothelin converting enzyme en-keyword=endothelin receptor kn-keyword=endothelin receptor en-keyword=epithelial cell kn-keyword=epithelial cell en-keyword=ovarian steroids kn-keyword=ovarian steroids en-keyword=oviductal contraction and relaxation kn-keyword=oviductal contraction and relaxation END start-ver=1.4 cd-journal=joma no-vol=29 cd-vols= no-issue=7 article-no= start-page=1280 end-page=1286 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160517 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum en-subtitle= kn-subtitle= en-abstract= kn-abstract= The corpus luteum (CL) synthesises and secretes progesterone (P4), which is essential for the establishment and maintenance of pregnancy in mammals. P4 is synthesised from cholesterol. Cholesterol is internalised by low-density lipoprotein receptor (LDLR) and/or scavenger receptor B1 (SR-BI), and is effluxed by ATP-binding cassette (ABC) transporter A1 (ABCA1) and G1 (ABCG1). To test the hypothesis that lipoprotein receptors and ABC transporters are involved in functional luteolysis, we examined the expression of LDLR, SR-BI, ABCA1 and ABCG1 in bovine CL during the luteal stages and after injection of prostaglandin (PG) F2α on Day 10 after ovulation. Expression of LDLR and SR-BI mRNA and protein was lower in the regressed luteal than late luteal stage. Injection of cows with a PGF2α did not affect LDLR mRNA and protein levels in the CL. Although expression of SR-BI mRNA did not change, SR-BI protein expression decreased 12 and 24 h after PGF2α injection. The overall findings of the present study suggest that the decreased expression of SR-BI induced by PGF2α is one of the factors responsible for the continuous decrease in P4 production during functional luteolysis. en-copyright= kn-copyright= en-aut-name=HorihataKei en-aut-sei=Horihata en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaShin en-aut-sei=Yoshioka en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SanoMasahiro en-aut-sei=Sano en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoYuki en-aut-sei=Yamamoto en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KimuraKoji en-aut-sei=Kimura en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SkarzynskiDariusz J. en-aut-sei=Skarzynski en-aut-mei=Dariusz J. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkudaKiyoshi en-aut-sei=Okuda en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University kn-affil= affil-num=2 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=6 en-affil=Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences kn-affil= affil-num=7 en-affil=Laboratory of Reproductive Physiology, Faculty of Agriculture, Okayama University kn-affil= en-keyword=luteal phase kn-keyword=luteal phase en-keyword=ovary kn-keyword=ovary en-keyword=progesterone kn-keyword=progesterone en-keyword=prostaglandin kn-keyword=prostaglandin en-keyword=reproduction kn-keyword=reproduction END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=22 article-no= start-page=e14640 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20201123 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions en-subtitle= kn-subtitle= en-abstract= kn-abstract=After parturition, cows frequently develop uterine bacterial infections, resulting in the onset of endometritis. To eliminate the bacteria, bovine endometrial cells secrete chemokines, such as IL-6 and MCP1, which attract macrophages (M Phi s) to the subepithelial stroma. These attracted M Phi s are not only involved in bacterial elimination but also the orchestration of inflammation and tissue repair. These immune responses aid in the recovery from endometritis; however, the recovery from endometritis takes longer in summer than in any other season. Based on these findings, we hypothesized that heat stress (HS) affects the chemokine production in endometrial cells. To confirm this hypothesis, we compared IL-6 and MCP1 production induced by lipopolysaccharide (LPS) in bovine endometrial epithelial and stromal cells under normal (38.5 degrees C) and HS conditions (40.5 degrees C). In the endometrial epithelial cells, IL-6 production stimulated by LPS was significantly (p < .05) suppressed under HS conditions. MCP1 production in endometrial epithelial cells was not detected under both the control and HS conditions regardless of the presence of LPS. Moreover, LPS significantly (p < .05) stimulated IL-6 and MCP1 production in endometrial stromal cells. Moreover, HS significantly (p < .05) enhanced their production compared to that under the control conditions. In addition, HS did not affect the migration ability of M Phi s; however, the supernatant of the endometrial stromal cells cultured under the HS condition significantly (p < .05) attracted the M Phi s when compared to the control condition. These results suggest that HS disrupts chemokine production in two types of endometrial cells and alters the distribution of M Phi s in the endometrium during the summer. en-copyright= kn-copyright= en-aut-name=SakaiShunsuke en-aut-sei=Sakai en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HatabuToshimitsu en-aut-sei=Hatabu en-aut-mei=Toshimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoYuki en-aut-sei=Yamamoto en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraKoji en-aut-sei=Kimura en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=chemokine kn-keyword=chemokine en-keyword=cow kn-keyword=cow en-keyword=endometrial cells kn-keyword=endometrial cells en-keyword=endometritis kn-keyword=endometritis en-keyword=heat stress kn-keyword=heat stress END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue= article-no= start-page=29 end-page=33 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Regulatory mechanisms for the expressions of local factors controlling bovine oviductal smooth muscle motility kn-title=ウシ卵管平滑筋運動を制御する局所 因子とその発現制御機構 en-subtitle= kn-subtitle= en-abstract= kn-abstract= Oviductal motility is required for transport of oocyte and embryo resulting in successful fertilization and implantation in mammals. The oviduct consists of epithelial, stromal and smooth muscle layers. Oviductal motility is systemically and locally regulated by various factors including prostaglandin F2 alpha (PGF) and endothelins (EDNs), and relaxing factors including prostaglandin E2 (PGE2) and nitric oxide (NO). The objective of our research is to clarify the regulatory system of oviductal motility including the production mechanisms of these factors in cattle. First, the expressions of regulating factors of oviductal motility were examined throughout the estrous cycle in the bovine oviduct. Some of them showed cyclical changes, which suggested that they were controlled by some other factors. Second, the effects of ovarian steroids or oviductal local factors on the expressions of PGs, EDNs and NO synthases were investigated using cell culture method. Several factors such as estradiol?17beta, progesterone and lysophosphatidic acid affected the expressions of regulating factors of smooth muscle motility. In addition, we found that these actions differed between the ampulla and isthmus in same types of cultured cell. Our studies suggest that regulatory factors of oviductal motility are produced during the optimal period and at proper location to transport the oocyte and early embryo in the bovine oviduct. Although the precise control of oviductal motility is essential for successful pregnancy, methods for diagnosing and treating of its functional abnormality have not been established yet not only in cows but also in other animals including human. Our studies should contribute to improving the fertility rates in mammals. en-copyright= kn-copyright= en-aut-name=YamamotoYuki en-aut-sei=Yamamoto en-aut-mei=Yuki kn-aut-name=山本ゆき kn-aut-sei=山本 kn-aut-mei=ゆき aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学農学部 en-keyword=ovarian steroid kn-keyword=ovarian steroid en-keyword=oviduct kn-keyword=oviduct en-keyword=prostaglandin kn-keyword=prostaglandin en-keyword=physiology kn-keyword=physiology en-keyword=smooth muscle motility kn-keyword=smooth muscle motility END start-ver=1.4 cd-journal=joma no-vol=69 cd-vols= no-issue=6 article-no= start-page=337 end-page=346 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=2023 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles en-subtitle= kn-subtitle= en-abstract= kn-abstract=Insulin-like growth factor-1 (IGF-1) plays a crucial role in follicular growth and stimulates steroid hormone production in bovine follicles. Steroid hormones are synthesized through the actions of steroidogenic enzymes, specifically STAR, CYP11A1, HSD3B, and CYP19A1 in both theca cells (TCs) and granulosa cells (GCs), under the influence of gonadotropins. Particularly, estradiol 17 beta (E2) assumes a central role in follicular development and selection by activating estrogen receptors beta (ESR2) in GCs. We assessed ESR2 mRNA expression in GCs of developing follicles and investigated the impact of IGF-1 on the mRNA expression of ESR2, CYP19A1, FSHR, and LHCGR, STAR, CYP11A1, and HSD17B in cultured GCs and TCs, respectively. Additionally, we assessed the influence of IGF-1 on androstenedione (A4), progesterone (P4), and testosterone (T) production in TCs. Small-sized follicles (< 6 mm) exhibited the highest levels of ESR2 mRNA expression, whereas medium-sized follicles (7-8 mm) displayed higher levels than large-sized follicles (>= 9 mm) (P < 0.05). IGF-1 increased the mRNA expression of ESR2, CYP19A1, and FSHR in GCs of follicles of both sizes, except for FSHR mRNA in medium-sized follicles (P < 0.05). IGF-1 significantly elevated mRNA expression of LHCGR, STAR, CYP11A1, and CYP17B in TCs of small-and medium-sized follicles (P < 0.05). Moreover, IGF-1 augmented the production of A4 and P4 but had no impact on T production in TCs of small-and medium-sized follicles. Taken together, our findings indicate that IGF-1 upregulates steroidogenic enzymes and steroid hormone production, underscoring the crucial role of IGF-1 in follicle development and selection. en-copyright= kn-copyright= en-aut-name=RawanAhmad Farid en-aut-sei=Rawan en-aut-mei=Ahmad Farid kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=LangarHikmatullah en-aut-sei=Langar en-aut-mei=Hikmatullah kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MunetomoMaho en-aut-sei=Munetomo en-aut-mei=Maho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoYuki en-aut-sei=Yamamoto en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawanoKohei en-aut-sei=Kawano en-aut-mei=Kohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimuraKoji en-aut-sei=Kimura en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=3 en-affil=Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=4 en-affil=Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=5 en-affil=Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=6 en-affil=Laboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= en-keyword=Estradiol receptor kn-keyword=Estradiol receptor en-keyword=Follicle kn-keyword=Follicle en-keyword=Insulin-like growth factor-1 (IGF-1) kn-keyword=Insulin-like growth factor-1 (IGF-1) en-keyword=Steroidogenic enzymes kn-keyword=Steroidogenic enzymes END