start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20060803 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Simultaneous gene transfer of bone morphogenetic protein (BMP)-2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Background: Transcutaneous in vivo electroporation is expected to be an effective gene-transfer method for promoting bone regeneration using the BMP-2 plasmid vector. To promote enhanced osteoinduction using this method, we simultaneously transferred cDNAs for BMP-2 and BMP-7, as inserts in the non-viral vector pCAGGS.
Methods: First, an in vitro study was carried out to confirm the expression of BMP-2 and BMP-7 following the double-gene transfer. Next, the individual BMP-2 and BMP-7 plasmids or both together were injected into rat calf muscles, and transcutaneous electroporation was applied 8 times at 100 V, 50 msec.
Results: In the culture system, the simultaneous transfer of the BMP-2 and BMP-7 genes led to a much higher ALP activity in C2C12 cells than did the transfer of either gene alone. In vivo, ten days after the treatment, soft X-ray analysis showed that muscles that received both pCAGGS-BMP-2 and pCAGGS-BMP-7 had better-defined opacities than those receiving a single gene. Histological examination showed advanced ossification in calf muscles that received the double-gene transfer. BMP-4 mRNA was also expressed, and RT-PCR showed that its level increased for 3 days in a timedependent manner in the double-gene transfer group. Immunohistochemistry confirmed that BMP- 4-expressing cells resided in the matrix between muscle fibers.
Conclusion: The simultaneous transfer of BMP-2 and BMP-7 genes using in vivo electroporation induces more rapid bone formation than the transfer of either gene alone, and the increased expression of endogenous BMP-4 suggests that the rapid ossification is related to the induction of BMP-4.

en-copyright= kn-copyright= en-aut-name=KawaiMariko en-aut-sei=Kawai en-aut-mei=Mariko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BesshoKazuhisa en-aut-sei=Bessho en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MaruyamaHiroki en-aut-sei=Maruyama en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyazakiJun-ichi en-aut-sei=Miyazaki en-aut-mei=Jun-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamamotoToshio en-aut-sei=Yamamoto en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University affil-num=3 en-affil= kn-affil=Division of Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences affil-num=4 en-affil= kn-affil=Division of Stem Cell Regulation Research, Osaka University Medical School affil-num=5 en-affil= kn-affil=Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page=24 end-page=27 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198804 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=鳥類の卵殻腺におけるカルシウム分泌細胞 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamamotoToshio en-aut-sei=Yamamoto en-aut-mei=Toshio kn-aut-name=山本敏男 kn-aut-sei=山本 kn-aut-mei=敏男 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学歯学部口腔解剖学第一講座 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue= article-no= start-page=61 end-page=66 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199912 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Case of Recurrent Churg-Strauss Syndrome Associated with Bronchial Asthma kn-title=Churg-Strauss症候群の再燃を来した気管支喘息症例 en-subtitle= kn-subtitle= en-abstract=A recurrent case of Churg-Strauss syndrome (CSS) associated with bronchial asthma is reported. A 73-year-old woman with bronchial asthma was admitted to our hospital because of persistent sensory disturbance of the bilateral lower limbs. About 2 weeks or more after admission, slight fever and an unpleasant sensation in the lower abdomen appeared and continued. Several days later, drop foot and melena developed. The eosinophil count was markedly increased and the endoscopic examination revealed rectal ulcers. Since the patient had a same experience of these symptoms three years ago, the recurrence of CSS was strongly suggested. Immediately after steroid treatment was started, these newly developed symptoms were relieved with no segulae. We reported the present case, which may be useful for clarifying the onset of CSS, with a review of the literature. kn-abstract=入院中にChurg-Strauss症候群の再燃を来した症例を報告した｡症例は気管支喘息を有する73歳の女性で,持続する両下肢の知覚障害を主訴に当院に入院した｡入院2週頃より微熱,下腹部不快感が続き右下肢のdrop foot,下血を呈した｡末梢血では著明な好酸球増多を認め,内視鏡検査で直腸潰瘍を認めた。3年前の両下肢の知覚障害出現時にも同様の症状を経験しており,Churg-Strauss症候群の再燃と診断し,ステロイド治療を開始することにより新たな後遺症を残すことなく寛解に至った｡Churg-Strauss症候群の発症･再燃の機序を考える上で貴重な症例と考え,文献的考察を加え報告する｡ en-copyright= kn-copyright= en-aut-name=HaradaSeishi en-aut-sei=Harada en-aut-mei=Seishi kn-aut-name=原田誠之 kn-aut-sei=原田 kn-aut-mei=誠之 aut-affil-num=1 ORCID= en-aut-name=TsugenoHirofumi en-aut-sei=Tsugeno en-aut-mei=Hirofumi kn-aut-name=柘野浩史 kn-aut-sei=柘野 kn-aut-mei=浩史 aut-affil-num=2 ORCID= en-aut-name=MitsunobuFumihiro en-aut-sei=Mitsunobu en-aut-mei=Fumihiro kn-aut-name=光延文裕 kn-aut-sei=光延 kn-aut-mei=文裕 aut-affil-num=3 ORCID= en-aut-name=MifuneTakashi en-aut-sei=Mifune en-aut-mei=Takashi kn-aut-name=御舩尚志 kn-aut-sei=御舩 kn-aut-mei=尚志 aut-affil-num=4 ORCID= en-aut-name=HosakiYasuhiro en-aut-sei=Hosaki en-aut-mei=Yasuhiro kn-aut-name=保崎泰弘 kn-aut-sei=保崎 kn-aut-mei=泰弘 aut-affil-num=5 ORCID= en-aut-name=AshidaKozo en-aut-sei=Ashida en-aut-mei=Kozo kn-aut-name=芦田耕三 kn-aut-sei=芦田 kn-aut-mei=耕三 aut-affil-num=6 ORCID= en-aut-name=OkamotoMakoto en-aut-sei=Okamoto en-aut-mei=Makoto kn-aut-name=岡本誠 kn-aut-sei=岡本 kn-aut-mei=誠 aut-affil-num=7 ORCID= en-aut-name=TakataShingo en-aut-sei=Takata en-aut-mei=Shingo kn-aut-name=高田真吾 kn-aut-sei=高田 kn-aut-mei=真吾 aut-affil-num=8 ORCID= en-aut-name=YamamotoToshio en-aut-sei=Yamamoto en-aut-mei=Toshio kn-aut-name=山本敏雄 kn-aut-sei=山本 kn-aut-mei=敏雄 aut-affil-num=9 ORCID= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=6 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=7 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=8 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 affil-num=9 en-affil= kn-affil=野島病院外科 affil-num=10 en-affil= kn-affil=岡山大学医学部附属病院三朝分院内科 en-keyword=Churg-Strauss症候群 (Churg-Strauss syndrome) kn-keyword=Churg-Strauss症候群 (Churg-Strauss syndrome) en-keyword=アレルギー性肉芽腫性血管炎 (allergic granulomatous angiitis) kn-keyword=アレルギー性肉芽腫性血管炎 (allergic granulomatous angiitis) en-keyword=血管炎症候群 (vasculitis syndrome) kn-keyword=血管炎症候群 (vasculitis syndrome) en-keyword=気管支喘息 (bronchial asthma) kn-keyword=気管支喘息 (bronchial asthma) en-keyword=好酸球増多 (eosinophilia) kn-keyword=好酸球増多 (eosinophilia) END