start-ver=1.4
cd-journal=joma
no-vol=19
cd-vols=
no-issue=10
article-no=
start-page=e0310962
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20241023
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Examination of yield, bacteriolytic activity and cold storage of linker deletion mutants based on endolysin S6_ORF93 derived from Staphylococcus giant bacteriophage S6
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Methicillin-resistant Staphylococcus spp. present challenges in clinical and veterinary settings because effective antimicrobial agents are limited. Phage-encoded peptidoglycan-degrading enzyme, endolysin, is expected to be a novel antimicrobial agent. The enzymatic activity has recently been shown to be influenced by the linker between functional domains in the enzyme. S6_ORF93 (ORF93) is one of the endolysins derived from previously isolated Staphylococcus giant phage S6. The ORF93 was speculated to have a catalytic and peptidoglycan-binding domain with a long linker. In this study, we examined the influence of linker shortening on the characteristics of ORF93. We produce wild-type ORF93 and the linker deletion mutants using an Escherichia coli expression system. These mutants were designated as ORF93-Delta 05, ORF93-Delta 10, ORF93-Delta 15, and ORF93-Delta 20, from which 5, 10, 15, and 20 amino acids were removed from the linker, respectively. Except for the ORF93-Delta 20, ORF93 and its mutants were expressed as soluble proteins. Moreover, ORF93-Delta 15 showed the highest yield and bacteriolytic activity, while the antimicrobial spectrum was homologous. The cold storage experiment showed a slight effect by the linker deletion. According to our results and other studies, linker investigations are crucial in endolysin development.
en-copyright=
kn-copyright=
en-aut-name=MunetomoSosuke
en-aut-sei=Munetomo
en-aut-mei=Sosuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Takemura-UchiyamaIyo
en-aut-sei=Takemura-Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WanganuttaraThamonwan
en-aut-sei=Wanganuttara
en-aut-mei=Thamonwan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=YamamotoYumiko
en-aut-sei=Yamamoto
en-aut-mei=Yumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TsukuiToshihiro
en-aut-sei=Tsukui
en-aut-mei=Toshihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=HagiyaHideharu
en-aut-sei=Hagiya
en-aut-mei=Hideharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KanamaruShuji
en-aut-sei=Kanamaru
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=KandaHideyuki
en-aut-sei=Kanda
en-aut-mei=Hideyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsushitaOsamu
en-aut-sei=Matsushita
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Public Health, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=6
en-affil=Nippon Zenyaku Kogyo Co. Ltd.
kn-affil=
affil-num=7
en-affil=Department of Infectious Diseases, Okayama University Hospital
kn-affil=
affil-num=8
en-affil=School of Life Science and Technology, Tokyo Institute of Technology
kn-affil=
affil-num=9
en-affil=Department of Public Health, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=10
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
END
start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=1
article-no=
start-page=31
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230916
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Exploratory study of volatile fatty acids and the rumen-and-gut microbiota of dairy cows in a single farm, with respect to subclinical infection with bovine leukemia virus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background Subclinical infection with bovine leukemia virus (BLV) in cows can cause economic losses in milk and meat production in many countries, as BLV-related negative effects. The volatile fatty acids (VFAs) and microbiota present in the digestive tracts of cows can contribute to cow health. Here, we exploratorily investigated the VFAs and microbiota in the rumen and gut with respect to subclinical BLV infection using cows housed at a single farm.
Results We analyzed a herd of 38 cows kept at one farm, which included 15 uninfected and 23 BLV-infected cows. First, the analysis of the VFAs in the rumen, gut, and blood revealed an absence of statistically significant differences between the uninfected and BLV-infected groups. Thus, BLV infection did not cause major changes in VFA levels in all tested specimens. Next, we analyzed the rumen and gut microbiota. The analysis of the microbial diversity revealed a modest difference between the uninfected and BLV-infected groups in the gut; by contrast, no differences were observed in the rumen. In addition, the investigation of the bacteria that were predominant in the uninfected and BLV-infected groups via a differential abundance analysis showed that no significant bacteria were present in either of the microbiota. Thus, BLV infection possibly affected the gut microbiota to a small extent. Moreover, bacterial associations were compared between the uninfected and BLV-infected groups. The results of this analysis suggested that BLV infection affected the equilibrium of the bacterial associations in both microbiota, which might be related to the BLV-related negative effects. Thus, BLV infection may negatively affect the equilibrium of bacterial associations in both microbiota.
Conclusions Subclinical BLV infection is likely to affect the rumen and gut microbiota, which may partly explain the BLV-related negative effects.
en-copyright=
kn-copyright=
en-aut-name=SuzukiTakehito
en-aut-sei=Suzuki
en-aut-mei=Takehito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MurakamiHironobu
en-aut-sei=Murakami
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=SatoReiichiro
en-aut-sei=Sato
en-aut-mei=Reiichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=Takemura-UchiyamaIyo
en-aut-sei=Takemura-Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OgataMasaya
en-aut-sei=Ogata
en-aut-mei=Masaya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=SogawaKazuyuki
en-aut-sei=Sogawa
en-aut-mei=Kazuyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=IshidaHiroho
en-aut-sei=Ishida
en-aut-mei=Hiroho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=AtipairinApichart
en-aut-sei=Atipairin
en-aut-mei=Apichart
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsushitaOsamu
en-aut-sei=Matsushita
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=NagaiMakoto
en-aut-sei=Nagai
en-aut-mei=Makoto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
affil-num=1
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=2
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=3
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Faculty of Agriculture, University of Miyazaki
kn-affil=
affil-num=5
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=6
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=7
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=8
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=9
en-affil=School of Pharmacy, Walailak University
kn-affil=
affil-num=10
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=11
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
en-keyword=Bovine leukemia virus
kn-keyword=Bovine leukemia virus
en-keyword=Volatile fatty acids
kn-keyword=Volatile fatty acids
en-keyword=Rumen
kn-keyword=Rumen
en-keyword=Gut, Microbiota
kn-keyword=Gut, Microbiota
en-keyword=Cows
kn-keyword=Cows
END
start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=3
article-no=
start-page=e04764-22
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230426
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The deterioration in reproductive performance in association with low fertility leads to significant economic losses on dairy farms. The uterine microbiota has begun to attract attention as a possible cause of unexplained low fertility. We analyzed the uterine microbiota associated with fertility by 16S rRNA gene amplicon sequencing in dairy cows. First, the alpha (Chao1 and Shannon) and beta (unweighted and weighted UniFrac) diversities of 69 cows at four dairy farms that had passed the voluntary waiting period before the first artificial insemination (AI) were analyzed with respect to factors including farm, housing style, feeding management, parity, and AI frequency to conception. Significant differences were observed in the farm, housing style, and feeding management, except parity and AI frequency to conception. The other diversity metrics did not show significant differences in the tested factors. Similar results were obtained for the predicted functional profile. Next, the microbial diversity analysis of 31 cows at a single farm using weighted UniFrac distance matrices revealed a correlation with AI frequency to conception but not with parity. In correlation with AI frequency to conception, the predicted function profile appeared to be slightly modified and a single bacterial taxon, Arcobacter, was detected. The bacterial associations related to fertility were estimated. Considering these, the uterine microbiota in dairy cows can be varied depending on the farm management practices and may become one of the measures for low fertility.
en-copyright=
kn-copyright=
en-aut-name=YagisawaTakuya
en-aut-sei=Yagisawa
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Takemura-UchiyamaIyo
en-aut-sei=Takemura-Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=AndoShun
en-aut-sei=Ando
en-aut-mei=Shun
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=IchiiOsamu
en-aut-sei=Ichii
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MurakamiHironobu
en-aut-sei=Murakami
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MatsushitaOsamu
en-aut-sei=Matsushita
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KatagiriSeiji
en-aut-sei=Katagiri
en-aut-mei=Seiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Hokkaido Agriculture Mutual Aid Association
kn-affil=
affil-num=2
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Hokkaido Agriculture Mutual Aid Association
kn-affil=
affil-num=5
en-affil=Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University
kn-affil=
affil-num=6
en-affil=Laboratory of Infectious Diseases, School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=7
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=8
en-affil=Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University
kn-affil=
en-keyword=dairy cows
kn-keyword=dairy cows
en-keyword=low fertility
kn-keyword=low fertility
en-keyword=uterine microbiota
kn-keyword=uterine microbiota
en-keyword=microbial diversity
kn-keyword=microbial diversity
en-keyword=bacterial association
kn-keyword=bacterial association
END
start-ver=1.4
cd-journal=joma
no-vol=84
cd-vols=
no-issue=7
article-no=
start-page=1019
end-page=1022
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=2022
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Screening of bacterial DNA in bile sampled from healthy dogs and dogs suffering from liver- or gallbladder-associated disease
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver- or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCR-positive samples showed that the microbial diversity differed between liver- and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs.
en-copyright=
kn-copyright=
en-aut-name=NEOSakurako
en-aut-sei=NEO
en-aut-mei=Sakurako
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TAKEMURA-UCHIYAMAIyo
en-aut-sei=TAKEMURA-UCHIYAMA
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=UCHIYAMAJumpei
en-aut-sei=UCHIYAMA
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MURAKAMIHironobu
en-aut-sei=MURAKAMI
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SHIMAAyaka
en-aut-sei=SHIMA
en-aut-mei=Ayaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KAYANUMAHideki
en-aut-sei=KAYANUMA
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=YOKOYAMATaiki
en-aut-sei=YOKOYAMA
en-aut-mei=Taiki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=TAKAGISatoshi
en-aut-sei=TAKAGI
en-aut-mei=Satoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=KANAIEiichi
en-aut-sei=KANAI
en-aut-mei=Eiichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=HISASUEMasaharu
en-aut-sei=HISASUE
en-aut-mei=Masaharu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=2
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=5
en-affil=Anicom Specialty Medical Institute Inc.
kn-affil=
affil-num=6
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=7
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=8
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=9
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=10
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
en-keyword=bile
kn-keyword=bile
en-keyword=bile microbiota
kn-keyword=bile microbiota
en-keyword=gallbladder
kn-keyword=gallbladder
en-keyword=hospitalized dog
kn-keyword=hospitalized dog
en-keyword=laboratory dog
kn-keyword=laboratory dog
END
start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=6
article-no=
start-page=1607
end-page=1616
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221201
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Characterization of the oral and fecal microbiota associated with atopic dermatitis in dogs selected from a purebred Shiba Inu colony
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Atopic dermatitis (AD) is a chronic and relapsing multifactorial inflammatory skin disease that also affects dogs. The oral and gut microbiota are associated with many disorders, including allergy. Few studies have addressed the oral and gut microbiota in dogs, although the skin microbiota has been studied relatively well in these animals. Here, we studied the AD-associated oral and gut microbiota in 16 healthy and nine AD dogs from a purebred Shiba Inu colony. We found that the diversity of the oral microbiota was significantly different among the dogs, whereas no significant difference was observed in the gut microbiota. Moreover, a differential abundance analysis detected the Family_XIII_AD3011_group (Anaerovoracaceae) in the gut microbiota of AD dogs; however, no bacterial taxa were detected in the oral microbiota. Third, the comparison of the microbial co-occurrence patterns between AD and healthy dogs identified differential networks in which the bacteria in the oral microbiota that were most strongly associated with AD were related with human periodontitis, whereas those in the gut microbiota were related with dysbiosis and gut inflammation. These results suggest that AD can alter the oral and gut microbiota in dogs.
en-copyright=
kn-copyright=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OsumiTakafumi
en-aut-sei=Osumi
en-aut-mei=Takafumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MizukamiKeijiro
en-aut-sei=Mizukami
en-aut-mei=Keijiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=FukuyamaTomoki
en-aut-sei=Fukuyama
en-aut-mei=Tomoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=ShimaAyaka
en-aut-sei=Shima
en-aut-mei=Ayaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=UnnoAsaka
en-aut-sei=Unno
en-aut-mei=Asaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=Takemura‐UchiyamaIyo
en-aut-sei=Takemura‐Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=UneYumi
en-aut-sei=Une
en-aut-mei=Yumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=MurakamiHironobu
en-aut-sei=Murakami
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=SakaguchiMasahiro
en-aut-sei=Sakaguchi
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Laboratory of Veterinary Internal Medicine, Division of Animal Life Science, Graduate School, Tokyo University of Agriculture and Technology
kn-affil=
affil-num=3
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=4
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=5
en-affil=Anicom Specialty Medical Institute Inc.
kn-affil=
affil-num=6
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=7
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=8
en-affil=Faculty of Veterinary Medicine, Okayama University of Science
kn-affil=
affil-num=9
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=10
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
en-keyword=oral
kn-keyword=oral
en-keyword=gut
kn-keyword=gut
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=atopic dermatitis
kn-keyword=atopic dermatitis
en-keyword=Shiba Inu
kn-keyword=Shiba Inu
en-keyword=dog colony
kn-keyword=dog colony
en-keyword=canine
kn-keyword=canine
END
start-ver=1.4
cd-journal=joma
no-vol=319
cd-vols=
no-issue=
article-no=
start-page=198881
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20221002
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Phylogenic analysis of new viral cluster of large phages with unusual DNA genomes containing uracil in place of thymine in gene-sharing network, using phages S6 and PBS1 and relevant uncultured phages derived from sewage metagenomics
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Bacteriophages (phages) are the most diverse and abundant life-form on Earth. Jumbophages are phages with double-stranded DNA genomes longer than 200 kbp. Among these, some jumbophages with uracil in place of thymine as a nucleic acid base, which we have tentatively termed "dU jumbophages" in this study, have been reported. Because the dU jumbophages are considered to be a living fossil from the RNA world, the evolutionary traits of dU jumbophages are of interest. In this study, we examined the phylogeny of dU jumbophages. First, tBLASTx analysis of newly sequenced dU jumbophages such as Bacillus phage PBS1 and previously isolated Staphylococcus phage S6 showed similarity to the other dU jumbophages. Second, we detected the two partial genome sequences of uncultured phages possibly relevant to dU jumbophages, scaffold_002 and scaffold_007, from wastewater metagenomics. Third, according to the gene-sharing network analysis, the dU jumbophages, including phages PBS1 and S6, and uncultured phage scaffold_002 formed a cluster, which suggested a new viral subfamily/family. Finally, analyses of the phylogenetic relationship with other phages showed that the dU jumbophage cluster, which had two clades of phages infecting Gram-negative and Gram-positive bacteria, diverged from the single ancestral phage. These findings together with previous reports may imply that dU jumbophages evolved from the same origin before divergence of Gram-negative and Gram-positive bacteria.
en-copyright=
kn-copyright=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=Takemura-UchiyamaIyo
en-aut-sei=Takemura-Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=GotohKazuyoshi
en-aut-sei=Gotoh
en-aut-mei=Kazuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KatoShin-ichiro
en-aut-sei=Kato
en-aut-mei=Shin-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SakaguchiYoshihiko
en-aut-sei=Sakaguchi
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MurakamiHironobu
en-aut-sei=Murakami
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=FukuyamaTomoki
en-aut-sei=Fukuyama
en-aut-mei=Tomoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=KanekiMao
en-aut-sei=Kaneki
en-aut-mei=Mao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=MatsushitaOsamu
en-aut-sei=Matsushita
en-aut-mei=Osamu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=MatsuzakiShigenobu
en-aut-sei=Matsuzaki
en-aut-mei=Shigenobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
affil-num=1
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=4
en-affil=Research Institute of Molecular Genetics, Kochi University
kn-affil=
affil-num=5
en-affil=Department of Microbiology, Kitasato University School of Medicine
kn-affil=
affil-num=6
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=7
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=8
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=9
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=10
en-affil=Department of Medical Laboratory Science, Faculty of Health Sciences, Kochi Gakuen University
kn-affil=
en-keyword=Environmental virus
kn-keyword=Environmental virus
en-keyword=Jumbophage
kn-keyword=Jumbophage
en-keyword=Metagenomics
kn-keyword=Metagenomics
en-keyword=Evolution
kn-keyword=Evolution
en-keyword=Uncultured phage
kn-keyword=Uncultured phage
END
start-ver=1.4
cd-journal=joma
no-vol=369
cd-vols=
no-issue=1
article-no=
start-page=fnac019
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=2022
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Heterogeneous IgE reactivities to Staphylococcus pseudintermedius strains in dogs with atopic dermatitis, and the identification of DM13-domain-containing protein as a bacterial IgE-reactive molecule
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Staphylococcus pseudintermedius is one of the major pathogens causing canine skin infection. In canine atopic dermatitis (AD), heterogeneous strains of S. pseudintermedius reside on the affected skin site. Because an increase in specific IgE to this bacterium has been reported, S. pseudintermedius is likely to exacerbate the severity of canine AD. In this study, the IgE reactivities to various S. pseudintermedius strains and the IgE-reactive molecules of S. pseudintermedius were investigated. First, examining the IgE reactivities to eight strains of S. pseudintermedius using 141 sera of AD dogs, strain variation of S. pseudintermedius showed 10–63% of the IgE reactivities. This is different from the expected result based on the concept of Staphylococcus aureus clonality in AD patients. Moreover, according to the western blot analysis, there were more than four proteins reactive to IgE. Subsequently, the analysis of the common IgE-reactive protein at ∼15 kDa confirmed that the DM13-domain-containing protein was reactive in AD dogs, which is not coincident with any S. aureus IgE-reactive molecules. Considering these, S. pseudintermedius is likely to exacerbate AD severity in dogs, slightly different from the case of S. aureus in human AD.
en-copyright=
kn-copyright=
en-aut-name=Takemura-UchiyamaIyo
en-aut-sei=Takemura-Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TsuruiHiroki
en-aut-sei=Tsurui
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ShimakuraHidekatsu
en-aut-sei=Shimakura
en-aut-mei=Hidekatsu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NasukawaTadahiro
en-aut-sei=Nasukawa
en-aut-mei=Tadahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=ImanishiIchiro
en-aut-sei=Imanishi
en-aut-mei=Ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=FukuyamaTomoki
en-aut-sei=Fukuyama
en-aut-mei=Tomoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=SakamotoShuji
en-aut-sei=Sakamoto
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=MorisawaKeiko
en-aut-sei=Morisawa
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=FujimuraMasato
en-aut-sei=Fujimura
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=MurakamiHironobu
en-aut-sei=Murakami
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=KanamaruShuji
en-aut-sei=Kanamaru
en-aut-mei=Shuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=KurokawaKenji
en-aut-sei=Kurokawa
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=KawamotoKeiko
en-aut-sei=Kawamoto
en-aut-mei=Keiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
en-aut-name=IyoriKeita
en-aut-sei=Iyori
en-aut-mei=Keita
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=15
ORCID=
en-aut-name=SakaguchiMasahiro
en-aut-sei=Sakaguchi
en-aut-mei=Masahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=16
ORCID=
affil-num=1
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=2
en-affil=School of Veterinary Medicine, Azabu University, Fuchinobe 1-17-71
kn-affil=
affil-num=3
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=4
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=5
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=6
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama Universty
kn-affil=
affil-num=7
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=8
en-affil=Science Research Center, Kochi Medical School
kn-affil=
affil-num=9
en-affil=Science Research Center, Kochi Medical School
kn-affil=
affil-num=10
en-affil=Fujimura Animal Hospital
kn-affil=
affil-num=11
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=12
en-affil=Department of Life Science and Technology, Tokyo Institute of Technology
kn-affil=
affil-num=13
en-affil=Faculty of Pharmaceutical Sciences, Nagasaki International University
kn-affil=
affil-num=14
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
affil-num=15
en-affil=Vet Derm Tokyo, Dermatological and Laboratory Service for Animals
kn-affil=
affil-num=16
en-affil=School of Veterinary Medicine, Azabu University
kn-affil=
en-keyword=Staphylococcus pseudintermedius
kn-keyword=Staphylococcus pseudintermedius
en-keyword=atopic dermatitis
kn-keyword=atopic dermatitis
en-keyword= IgE
kn-keyword= IgE
en-keyword=dogs
kn-keyword=dogs
en-keyword=DM13-domain-containing protein
kn-keyword=DM13-domain-containing protein
en-keyword=exacerbation factor
kn-keyword=exacerbation factor
END
start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=1
article-no=
start-page=e00077-21
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202193
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Use of Recombinant Endolysin to Improve Accuracy of Group B Streptococcus Tests
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Group B Streptococcus (GBS) causes serious neonatal infection via vertical transmission. The prenatal GBS screening test is performed at the late stage of pregnancy to avoid risks of infection. In this test, enrichment culture is performed, followed by GBS identification. Selective medium is used for the enrichment; however, Enterococcus faecalis, which is a potential contaminant in swab samples, can interfere with the growth of GBS. Such bacterial contamination can lead to false-negative results. Endolysin, a bacteriophage-derived enzyme, degrades peptidoglycan in the bacterial cell wall; it is a promising antimicrobial agent for selectively eliminating specific bacterial genera/species. In this study, we used the recombinant endolysin EG-LYS, which is specific to E. faecalis; the endolysin potentially enriched GBS in the selective culture. First, in the false-negative model (coculture of GBS and E. faecalis, which disabled GBS detection in the subsequent GBS identification test), EG-LYS treatment at 0.1 mg/ml improved GBS detection. Next, we used 548 vaginal swabs to test the efficacy of EG-LYS treatment in improving GBS detection. EG-LYS treatment (0.1 mg/ml) increased the GBS-positive ratio to 17.9%, compared to 15.7% in the control (phosphate-buffered saline [PBS] treatment). In addition, there were an increased number of GBS colonies under EG-LYS treatment in some samples. The results were supported by the microbiota analysis of the enriched cultures. In conclusion, EG-LYS treatment of the enrichment culture potentially improves the accuracy of the prenatal GBS screening test.
en-copyright=
kn-copyright=
en-aut-name=MatsuiHidehito
en-aut-sei=Matsui
en-aut-mei=Hidehito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=UchiyamaJumpei
en-aut-sei=Uchiyama
en-aut-mei=Jumpei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=OgataMasaya
en-aut-sei=Ogata
en-aut-mei=Masaya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NasukawaTadahiro
en-aut-sei=Nasukawa
en-aut-mei=Tadahiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=Takemura-UchiyamaIyo
en-aut-sei=Takemura-Uchiyama
en-aut-mei=Iyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KatoShin-ichiro
en-aut-sei=Kato
en-aut-mei=Shin-ichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=MurakamiHironobu
en-aut-sei=Murakami
en-aut-mei=Hironobu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=HigashideMasato
en-aut-sei=Higashide
en-aut-mei=Masato
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=HanakiHideaki
en-aut-sei=Hanaki
en-aut-mei=Hideaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
affil-num=1
en-affil=Ōmura Satoshi Memorial Institute, Kitasato University
kn-affil=
affil-num=2
en-affil=Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=
affil-num=3
en-affil=School of Veterinary Medicine, Azabu University, Sagamihara
kn-affil=
affil-num=4
en-affil=School of Veterinary Medicine, Azabu University, Sagamihara
kn-affil=
affil-num=5
en-affil=School of Veterinary Medicine, Azabu University, Sagamihara
kn-affil=
affil-num=6
en-affil=Kochi University
kn-affil=
affil-num=7
en-affil=School of Veterinary Medicine, Azabu University, Sagamihara
kn-affil=
affil-num=8
en-affil=Kotobiken Medical Laboratories, Inc., Tsukuba
kn-affil=
affil-num=9
en-affil=Ōmura Satoshi Memorial Institute, Kitasato University
kn-affil=
END