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ID 61290
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Author
Tumewu, Stephany Angelia Graduate School of Environmental and Life Science, Okayama University
Matsui, Hidenori Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life Science, Okayama University Kaken ID publons researchmap
Noutoshi, Yoshiteru Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Ichinose, Yuki Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Abstract
γ-Aminobutyric acid (GABA) is a widely distributed non-proteinogenic amino acid that accumulates in plants under biotic and abiotic stress conditions. Recent studies suggested that GABA also functions as an intracellular signaling molecule in plants and in signals mediating interactions between plants and phytopathogenic bacteria. However, the molecular mechanisms underlying GABA responses to bacterial pathogens remain unknown. In the present study, a GABA receptor, named McpG, was conserved in the highly motile plant-pathogenic bacteria Pseudomonas syringae pv. tabaci 6605 (Pta6605). We generated a deletion mutant of McpG to further investigate its involvement in GABA chemotaxis using quantitative capillary and qualitative plate assays. The wild-type strain of Pta6605 was attracted to GABA, while the ΔmcpG mutant abolished chemotaxis to 10‍ ‍mM GABA. However, ΔmcpG retained chemotaxis to proteinogenic amino acids and succinic semialdehyde, a structural analog of GABA. Furthermore, ΔmcpG was unable to effectively induce disease on host tobacco plants in three plant inoculation assays: flood, dip, and infiltration inoculations. These results revealed that the GABA sensing of Pta6605 is important for the interaction of Pta6605 with its host tobacco plant.
Keywords
bacterial virulence
chemotaxis
GABA
plant-microbe interaction
Pseudomonas
Published Date
2020
Publication Title
Microbes and Environments
Volume
volume35
Issue
issue4
Publisher
Japanese Society of Microbial Ecology
Start Page
ME20114
ISSN
1342-6311
NCID
AA11173196
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles.
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publisher
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1264/jsme2.ME20114
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
26660035
19H02956