start-ver=1.4 cd-journal=joma no-vol=150 cd-vols= no-issue=3 article-no= start-page=730 end-page=741 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080528 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Angiotensin II type 2 receptors facilitate reinnervation of phenol-lesioned vascular calcitonin gene-related peptide (CGRP)-containing nerves in rat mesenteric arteries en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The present study was designed to investigate involvement of angiotensin (Ang) II type 2 receptors (AT2 receptors) in restoration of perivascular nerve innervation injured by topical phenol treatment. Male Wistar rats underwent in vivo topical application of 10% phenol around the superior mesenteric artery. After phenol treatment, animals were subjected to immunohistochemistry of the third branch of small arteries, Western blot analysis of AT2 receptor protein expression in dorsal root ganglia (DRG) and studies of mesenteric neurogenic vasoresponsiveness. Ang II (750 ng/kg/day), nerve growth factor (NGF; 20 μg/kg/day) and PD123,319 (AT2 receptor antagonist; 10 mg/kg/day) were intraperitoneally administered for 7 days using osmotic mini-pumps immediately after topical phenol treatment. Losartan (AT1 receptor antagonist) was administered in drinking water (0.025%). Phenol treatment markedly reduced densities of both calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI)- and neuropeptide Y (NPY)-LI-containing fibers. NGF restored densities of both nerve fibers to the Sham control level. Coadministration of Ang II and losartan significantly increased the density of CGRP-LI-fibers but not NPY-LI-fibers compared with saline control. The increase of the density of CGRP-LI-fibers by coadministration of Ang II and losartan was suppressed by adding PD123,319. Coadministration of Ang II and losartan ameliorated reduction of CGRP nerve-mediated vasodilation of perfused mesenteric arteries caused by phenol treatment. The AT2 receptor protein expression detected in DRG was markedly increased by NGF. These results suggest that selective stimulation of AT2 receptors by Ang II facilitates reinnervation of mesenteric perivascular CGRP-containing nerves injured by topical phenol application in the rat.

en-copyright= kn-copyright= en-aut-name=HobaraNarumi en-aut-sei=Hobara en-aut-mei=Narumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=GodaMitsuhiro en-aut-sei=Goda en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YoshidaNamika en-aut-sei=Yoshida en-aut-mei=Namika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakatoriShingo en-aut-sei=Takatori en-aut-mei=Shingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitamuraYoshihisa en-aut-sei=Kitamura en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MioMitsunobu en-aut-sei=Mio en-aut-mei=Mitsunobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Shujitsu University School of Pharmacy affil-num=5 en-affil= kn-affil=Department of Pharmaceutical Care and Health Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=6 en-affil= kn-affil=Shujitsu University School of Pharmacy affil-num=7 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=Angiotensin II type 2 receptors kn-keyword=Angiotensin II type 2 receptors en-keyword=Phenol-induced perivascular nerve injury kn-keyword=Phenol-induced perivascular nerve injury en-keyword=Calcitonin gene-related peptide-containing nerves kn-keyword=Calcitonin gene-related peptide-containing nerves en-keyword=Neuropeptide Y-containing nerves kn-keyword=Neuropeptide Y-containing nerves en-keyword=Neurotrophic kn-keyword=Neurotrophic en-keyword=Rat mesenteric artery kn-keyword=Rat mesenteric artery END start-ver=1.4 cd-journal=joma no-vol=23 cd-vols= no-issue= article-no= start-page=33 end-page=35 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The pithed rats in research of the cardiovascular pharmacology kn-title=脊髄穿刺ラットを用いた循環薬理学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TakatoriShingo en-aut-sei=Takatori en-aut-mei=Shingo kn-aut-name=髙取真吾 kn-aut-sei=髙取 kn-aut-mei=真吾 aut-affil-num=1 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name=川﨑博己 kn-aut-sei=川﨑 kn-aut-mei=博己 aut-affil-num=2 ORCID= en-aut-name=MioMitsunobu en-aut-sei=Mio en-aut-mei=Mitsunobu kn-aut-name=見尾光庸 kn-aut-sei=見尾 kn-aut-mei=光庸 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=就実大学薬学部薬効解析学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学 affil-num=3 en-affil= kn-affil=就実大学薬学部薬効解析学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20040325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=インスリンの血圧調節機構に及ぼす影響に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TakatoriShingo en-aut-sei=Takatori en-aut-mei=Shingo kn-aut-name=髙取真吾 kn-aut-sei=髙取 kn-aut-mei=真吾 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END