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ID 60842
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Zhang, Rui Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University
Hisano, Sakae Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University
Tani, Akio Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Kondo, Hideki Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University Kaken ID publons researchmap
Kanematsu, Satoko NARO Institute of Fruit Tree Science
Suzuki, Nobuhiro Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Abstract
Viruses typically encode the capsid that encases their genome, while satellite viruses do not encode a replicase and depend on a helper virus for their replication1. Here, we report interplay between two RNA viruses, yado-nushi virus 1 (YnV1) and yado-kari virus 1 (YkV1), in a phytopathogenic fungus, Rosellinia necatrix2. YkV1 has a close phylogenetic affinity to positive-sense, single-stranded (+)ssRNA viruses such as animal caliciviruses3, while YnV1 has an undivided double-stranded (ds) RNA genome with a resemblance to fungal totiviruses4. Virion transfection and infectious full-length cDNA transformation has shown that YkV1 depends on YnV1 for viability, although it probably encodes functional RNA-dependent RNA polymerase (RdRp). Immunological and molecular analyses have revealed trans-encapsidation of not only YkV1 RNA but also RdRp by the capsid protein of the other virus (YnV1), and enhancement of YnV1 accumulation by YkV1. This study demonstrates interplay in which the capsidless (+)ssRNA virus (YkV1), hijacks the capsid protein of the dsRNA virus (YnV1), and replicates as if it were a dsRNA virus.
Keywords
Molecular evolution
Viral genetics
Published Date
2016-01-11
Publication Title
Nature Microbiology
Volume
volume1
Issue
issue1
Publisher
Nature Research
Start Page
15001
ISSN
2058-5276
NCID
AA1278421X
Content Type
Journal Article
language
English
OAI-PMH Set
岡山大学
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isVersionOf https://doi.org/10.1038/nmicrobiol.2015.1