start-ver=1.4 cd-journal=joma no-vol=85 cd-vols= no-issue=6 article-no= start-page=405 end-page=412 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190607 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A class III peroxidase PRX34 is a component of disease resistance in Arabidopsis en-subtitle= kn-subtitle= en-abstract= kn-abstract= PRX34 mediates the oxidative burst in Arabidopsis. Here we characterized two additional Arabidopsis prx34 null mutants (prx34-2, prx34-3), besides the well-studied prx34-1. Due to a decrease in corresponding peroxidase, the activity that generates reactive oxygen species (ROS) was significantly lower in cell wall extracts of prx34-2 and prx34-3 plants. Consistently, the prx34-2 and prx34-3 exhibited reduced accumulation both of ROS and callose in Flg22-elicitor-treated leaves, leading to enhanced susceptibility to bacterial and fungal pathogens. In contrast, ectopic expression of PRX34 in the wild type caused enhanced resistance. PRX34 is thus a component for disease resistance in Arabidopsis. en-copyright= kn-copyright= en-aut-name=ZhaoLei en-aut-sei=Zhao en-aut-mei=Lei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Le Thi Phuong en-aut-sei=Le Thi Phuong en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Mai Thanh Luan en-aut-sei=Mai Thanh Luan en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Aprilia Nur Fitrianti en-aut-sei=Aprilia Nur Fitrianti en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsuiHidenori en-aut-sei=Matsui en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NakagamiHirofumi en-aut-sei=Nakagami en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NoutoshiYoshiteru en-aut-sei=Noutoshi en-aut-mei=Yoshiteru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YamamotoMikihiro en-aut-sei=Yamamoto en-aut-mei=Mikihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=2 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=3 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=4 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=5 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=6 en-affil=RIKEN Center for Sustainable Resource Science kn-affil= affil-num=7 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=8 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=9 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=10 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= affil-num=11 en-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life ScienceOkayama University kn-affil= en-keyword=Apoplastic oxidative burst kn-keyword=Apoplastic oxidative burst en-keyword=Arabidopsis kn-keyword=Arabidopsis en-keyword=Cell wall kn-keyword=Cell wall en-keyword=Class III peroxidase kn-keyword=Class III peroxidase en-keyword=PRX34 kn-keyword=PRX34 en-keyword=Reactive oxygen species (ROS) kn-keyword=Reactive oxygen species (ROS) END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue= article-no= start-page=7 end-page=14 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A Volatile Substance, β-Caryophyllene, from Talaromyces wortmannii Promotes Growth and Tolerance to Diseases on Several Plants kn-title=β-caryophylleneの植物に対する生育促進作用 および耐病性増進作用の解析 en-subtitle= kn-subtitle= en-abstract=岡山県総社市の圃場から分離した植物生育促進菌Talaromyces wortmannii FS2が生産するβ-caryophylleneは,コマツナ(アブラナ科)のみならず,キュウリ(ウリ科),タバコ(ナス科)およびオオムギ(イネ科)など広汎な植物に対して,生育促進作用および耐病性増進作用を示したことから,有用な農業資材として利用可能であるものと考察した. kn-abstract=A plant growth-promoting fungus, Talaromyces wortmannii strain FS2 was isolated from an agricultural field at Okayama Pref. FS2 enhanced seed germination, root elongation and leaf growth of Brassica rapa var perviridis (Komatsuna). Such plant growth-promoting effect was observed in the same sealed chamber where FS2 was cultured on PDA medium separated from seedlings, suggesting effective volatile compound(s). GC‒MS analysis showed that FS2 emitted at least seven terpenoids, of which a volatile was identified as β‒caryophyllene. β‒caryophyllene alone promoted the growth of cucumber, Nicotiana benthamiana and barley. Furthermore β‒caryophyllene increased the yield of cucumber fruits. Interestingly, we found that β‒caryophyllene conditioned these plants to be resistant to respective diseases caused by Colletotrichum orbiculare, Botrytis cinerea or Blumeria graminis f. sp hordei. The findings indicate that β‒caryophyllene has desirable dual features and therefore, it is available to cultivation of many crops. en-copyright= kn-copyright= en-aut-name=YamagiwaYasuo en-aut-sei=Yamagiwa en-aut-mei=Yasuo kn-aut-name=山際泰夫 kn-aut-sei=山際 kn-aut-mei=泰夫 aut-affil-num=1 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=2 ORCID= en-aut-name=InagakiYoshishige en-aut-sei=Inagaki en-aut-mei=Yoshishige kn-aut-name=稲垣善茂 kn-aut-sei=稲垣 kn-aut-mei=善茂 aut-affil-num=3 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=4 ORCID= en-aut-name=HyakumachiaMitsuro en-aut-sei=Hyakumachia en-aut-mei=Mitsuro kn-aut-name=百町満朗 kn-aut-sei=百町 kn-aut-mei=満朗 aut-affil-num=5 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岐阜大学大学院 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=β-caryophyllene kn-keyword=β-caryophyllene en-keyword=plant growth-promoting kn-keyword=plant growth-promoting en-keyword=resistance induction kn-keyword=resistance induction en-keyword=Talaromyces wortmannii kn-keyword=Talaromyces wortmannii END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue= article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation and Identification of a Plant Growth-Promoting Fungus from an Agricultural Field in Okayama Prefecture kn-title=岡山県の栽培圃場における植物生育促進菌の探索と同定 en-subtitle= kn-subtitle= en-abstract=本研究では,実際の生産圃場から植物生育促進菌(PGPF)の探索を試み,コマツナの生育を促進するFS2株を分離した.FS2株の形態観察並びにのITS1領域の系統樹解析から本菌をTalaromyces wortmanniiと同定した. kn-abstract=A plant growth-promoting fungus was isolated from an agricultural field in Okayama Prefecture, Japan. The strain FS2, which enhanced seed germination, root elongation and leaf growth of Brassica rapa var. perviridis, was identified as Talaromyces wortmannii based on ITS1 sequence and its morphology. en-copyright= kn-copyright= en-aut-name=YamagiwaYasuo en-aut-sei=Yamagiwa en-aut-mei=Yasuo kn-aut-name=山際泰夫 kn-aut-sei=山際 kn-aut-mei=泰夫 aut-affil-num=1 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=2 ORCID= en-aut-name=InagakiYoshishige en-aut-sei=Inagaki en-aut-mei=Yoshishige kn-aut-name=稲垣善茂 kn-aut-sei=稲垣 kn-aut-mei=善茂 aut-affil-num=3 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=4 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Brassica rapa var. perviridis (Komatsuna) kn-keyword=Brassica rapa var. perviridis (Komatsuna) en-keyword=ITS1 region kn-keyword=ITS1 region en-keyword=Plant growth-promoting fungus kn-keyword=Plant growth-promoting fungus en-keyword=Talaromyces wortmannii (Penicillium kloeckeri) kn-keyword=Talaromyces wortmannii (Penicillium kloeckeri) en-keyword=volatile compounds kn-keyword=volatile compounds END start-ver=1.4 cd-journal=joma no-vol=279 cd-vols= no-issue=3 article-no= start-page=303 end-page=312 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080301 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.

en-copyright= kn-copyright= en-aut-name=HigashiKuniaki en-aut-sei=Higashi en-aut-mei=Kuniaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshigaYasuhiro en-aut-sei=Ishiga en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InagakiYoshishige en-aut-sei=Inagaki en-aut-mei=Yoshishige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University en-keyword=flagellin kn-keyword=flagellin en-keyword=flg22 kn-keyword=flg22 en-keyword=FLS2 kn-keyword=FLS2 en-keyword=MAMP signaling pathway kn-keyword=MAMP signaling pathway en-keyword=WRKY41 kn-keyword=WRKY41 END start-ver=1.4 cd-journal=joma no-vol=279 cd-vols= no-issue=4 article-no= start-page=313 end-page=322 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200804 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta gacA Delta gacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.

en-copyright= kn-copyright= en-aut-name=MarutaniMizuri en-aut-sei=Marutani en-aut-mei=Mizuri kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TaguchiFumiko en-aut-sei=Taguchi en-aut-mei=Fumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OgawaYujiro en-aut-sei=Ogawa en-aut-mei=Yujiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HossainMijan Md. en-aut-sei=Hossain en-aut-mei=Mijan Md. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YoshishigeInagaki en-aut-sei=Yoshishige en-aut-mei=Inagaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University en-keyword=GacA kn-keyword=GacA en-keyword=GacS kn-keyword=GacS en-keyword=Pseudomonas syringae pv. tabaci kn-keyword=Pseudomonas syringae pv. tabaci en-keyword=quorum sensing kn-keyword=quorum sensing en-keyword=two-component system kn-keyword=two-component system END start-ver=1.4 cd-journal=joma no-vol=18 cd-vols= no-issue=2 article-no= start-page=152 end-page=159 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 ( NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow ( BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein ( GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

en-copyright= kn-copyright= en-aut-name=SasabeMichiko en-aut-sei=Sasabe en-aut-mei=Michiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NaitoKana en-aut-sei=Naito en-aut-mei=Kana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuenagaHiroko en-aut-sei=Suenaga en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IkedaTakako en-aut-sei=Ikeda en-aut-mei=Takako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InagakiYoshishige en-aut-sei=Inagaki en-aut-mei=Yoshishige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University en-keyword=defense response kn-keyword=defense response en-keyword=elicitin kn-keyword=elicitin en-keyword=lectin kn-keyword=lectin en-keyword=receptor-like kinase kn-keyword=receptor-like kinase en-keyword=tobacco BY-2 kn-keyword=tobacco BY-2 END start-ver=1.4 cd-journal=joma no-vol=188 cd-vols= no-issue=24 article-no= start-page=8376 end-page=8384 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 deletion mutant (Delta orf3 mutant) had reduced virulence on tobacco plants. Orf3 shows significant homology to a 3-oxoacyl-(acyl carrier protein) synthase III in the fatty acid elongation cycle. The Delta orf3 mutant had a significantly reduced ability to form acyl homoserine lactones (AHLs), which are quorum-sensing molecules, suggesting that Orf3 is required for AHL synthesis. In comparison with the wild-type strain, swarming motility, biosurfactant production, and tolerance to H2O2 and antibiotics were enhanced in the Delta orf3 mutant. A scanning electron micrograph of inoculated bacteria on the tobacco leaf surface revealed that there is little extracellular polymeric substance matrix surrounding the cells in the Delta orf3 mutant. The phenotypes of the Delta orf3 mutant and an AHL synthesis (Delta psyI) mutant were similar, although the mutant-specific characteristics were more extreme in the Delta orf3 mutant. The swarming motility of the Delta orf3 mutant was greater than that of the Delta psyI mutant. This was attributed to the synergistic effects of the overproduction of biosurfactants and/or alternative fatty acid metabolism in the Delta orf3 mutant. Furthermore, the amounts of iron and biosurfactant seem to be involved in biofilm development under quorum-sensing regulation in P. syringae pv. tabaci 6605. en-copyright= kn-copyright= en-aut-name=TaguchiFumiko en-aut-sei=Taguchi en-aut-mei=Fumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OgawaYujiro en-aut-sei=Ogawa en-aut-mei=Yujiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakeuchiKasumi en-aut-sei=Takeuchi en-aut-mei=Kasumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SuzukiTomoko en-aut-sei=Suzuki en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University en-keyword=TO-CELL SIGNALS kn-keyword=TO-CELL SIGNALS en-keyword=AERUGINOSA kn-keyword=AERUGINOSA en-keyword=FLAGELLIN kn-keyword=FLAGELLIN en-keyword=BIOFILMS kn-keyword=BIOFILMS en-keyword=MOTILITY kn-keyword=MOTILITY en-keyword=IRON kn-keyword=IRON en-keyword=IDENTIFICATION kn-keyword=IDENTIFICATION en-keyword=SIDEROPHORES kn-keyword=SIDEROPHORES en-keyword=SPECIFICITY kn-keyword=SPECIFICITY en-keyword=FLUORESCENT kn-keyword=FLUORESCENT END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=6 article-no= start-page=923 end-page=938 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20066 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification of glycosylation genes and glycosylated amino acids of flagellin in Pseudomonas syringae pv. tabaci en-subtitle= kn-subtitle= en-abstract= kn-abstract=A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule. en-copyright= kn-copyright= en-aut-name=TaguchiFumiko en-aut-sei=Taguchi en-aut-mei=Fumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeuchiKasumi en-aut-sei=Takeuchi en-aut-mei=Kasumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KatohEtsuko en-aut-sei=Katoh en-aut-mei=Etsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurataKatsuyoshi en-aut-sei=Murata en-aut-mei=Katsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SuzukiTomoko en-aut-sei=Suzuki en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MarutaniMizuri en-aut-sei=Marutani en-aut-mei=Mizuri kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KawasakiTakayuki en-aut-sei=Kawasaki en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=EguchiMinako en-aut-sei=Eguchi en-aut-mei=Minako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KatohShizue en-aut-sei=Katoh en-aut-mei=Shizue kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=kakuHanae en-aut-sei=kaku en-aut-mei=Hanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=YasudaChihiro en-aut-sei=Yasuda en-aut-mei=Chihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=InagakiYoshishige en-aut-sei=Inagaki en-aut-mei=Yoshishige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= affil-num=1 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=4 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=7 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=9 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=10 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=11 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=12 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=13 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=14 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University affil-num=15 en-affil= kn-affil=The Graduate School of Natural Science and Technology, Okayama University en-keyword=Gram-Negative bacteria kn-keyword=Gram-Negative bacteria en-keyword=Posttranslational modification kn-keyword=Posttranslational modification en-keyword=Protein Glycosylation kn-keyword=Protein Glycosylation en-keyword=Perception kn-keyword=Perception en-keyword=Aeruginosa kn-keyword=Aeruginosa en-keyword=Cells kn-keyword=Cells en-keyword=Specificity kn-keyword=Specificity en-keyword=Expression kn-keyword=Expression en-keyword=Plasmids kn-keyword=Plasmids en-keyword=Pathways kn-keyword=Pathways END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=4 article-no= start-page=365 end-page=373 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070701 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Suppression of Cdc27B expression induces plant defence responses en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY- 2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase- promoting complex or cyclosome ( APC/ C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus:: Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.

en-copyright= kn-copyright= en-aut-name=KudoChikako en-aut-sei=Kudo en-aut-mei=Chikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SuzukiTomoko en-aut-sei=Suzuki en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FukuokaSumie en-aut-sei=Fukuoka en-aut-mei=Sumie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AsaiShuta en-aut-sei=Asai en-aut-mei=Shuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SuenagaHiroko en-aut-sei=Suenaga en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SasabeMichiko en-aut-sei=Sasabe en-aut-mei=Michiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakanoYoshitaka en-aut-sei=Takano en-aut-mei=Yoshitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OkunoTetsuro en-aut-sei=Okuno en-aut-mei=Tetsuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=InagakiYoshi-Shige en-aut-sei=Inagaki en-aut-mei=Yoshi-Shige kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=2 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=3 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=4 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=5 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=6 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=7 en-affil= kn-affil= affil-num=8 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=9 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=10 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=11 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University affil-num=12 en-affil= kn-affil=Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=27 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of FFC Ceramic Water on the Infection Process of a Fungal Pathogen kn-title=病原菌の感染行動に及ぼす FFC セラミック水の効果について en-subtitle= kn-subtitle= en-abstract=本研究は,FFCセラミックス(TM)(株 エフフシージャパン)の植物病原菌の発病抑制効果について調べたものである.FFCセラミック水は原液の1/2~1/6の濃度でエンドウ褐紋病菌の発病を顕著に抑制した.この原因を調べたところ,FFCセラミック水は,病原菌の発芽,発芽管伸長,侵入(貫入)を顕著に阻害することが判明した.FFCセラミック水中にはCa並びにS,O元素が多量に存在し,SEM観察の結果と合わせると,CaSO(4)が多量に含まれることが示唆された.そこで,CaSO(4)飽和液の1/2~1/4濃度で,病原菌に対する作用を調べた結果,発芽あるいは発芽管伸長はほとんど阻害されず,低率ながら侵入も観察された.これらの結果を総合して,FFCセラミック水やCaSO4の栽培場面での応用を考察した. kn-abstract=In this report, an effect of FFC-ceramic (FFC-Japan Co. Ltd., Tsu) water on the process of infection by a pea fungal pathogen, Mycosphaerella pinodes was investigated. Energy dispersive X-ray analysis showed that both of the FFC-ceramic water and a common ceramic water contained mainly Ca and S elements, of which the relative atomic percentages were 53~56% and 44~45%, respectively. Lesion formation by pycnospores of M. pinodes on pea leaves was inhibited severely by the application with both ceramic waters at the 1/2~1/6 concentration of saturated solution. Cytological observation under microscope showed that germination, germ-tube elongation and penetration were severely inhibited by these ceramic waters. However, such inhibitory effect of FFC-ceramic water was superior to that of the common ceramic water. On ethanol-killed pea epidermal tissues, both FFC-ceramic water and the common ceramic water blocked the germination, germ-tube elongation and penetration by the pathogen, indicating the direct effect of both ceramic waters on the fungus. In this case, the inhibiting effect of FFC ceramic water was more intensive than the common ceramic water. CaSO(4) at a 1/2~1/4 concentration of saturated solution blocked penetration by the fungus on the killed epidermis of onion bulb but scarcely affected germination and germ-tube elongation. Based on these results, we discussed the role of FFC-ceramic water in disease tolerance of plants and its availability for cultivation. en-copyright= kn-copyright= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=1 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=2 ORCID= en-aut-name=SuzukiTomoko en-aut-sei=Suzuki en-aut-mei=Tomoko kn-aut-name=鈴木智子 kn-aut-sei=鈴木 kn-aut-mei=智子 aut-affil-num=3 ORCID= en-aut-name=MeguroAkane en-aut-sei=Meguro en-aut-mei=Akane kn-aut-name=目黒あかね kn-aut-sei=目黒 kn-aut-mei=あかね aut-affil-num=4 ORCID= en-aut-name=HasegawaSachiko en-aut-sei=Hasegawa en-aut-mei=Sachiko kn-aut-name=長谷川幸子 kn-aut-sei=長谷川 kn-aut-mei=幸子 aut-affil-num=5 ORCID= en-aut-name=NishimuraTomio en-aut-sei=Nishimura en-aut-mei=Tomio kn-aut-name=西村富生 kn-aut-sei=西村 kn-aut-mei=富生 aut-affil-num=6 ORCID= en-aut-name=KunohHitoshi en-aut-sei=Kunoh en-aut-mei=Hitoshi kn-aut-name=久能均 kn-aut-sei=久能 kn-aut-mei=均 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=株式会社赤塚植物園・生物機能開発研究所 affil-num=5 en-affil= kn-affil=株式会社赤塚植物園・生物機能開発研究所 affil-num=6 en-affil= kn-affil=株式会社赤塚植物園・生物機能開発研究所 affil-num=7 en-affil= kn-affil=岡山大学 en-keyword=Calcium sulfate kn-keyword=Calcium sulfate en-keyword=FFC-ceramics kn-keyword=FFC-ceramics en-keyword=infection establishment kn-keyword=infection establishment en-keyword=Mycosphaerella pinodes kn-keyword=Mycosphaerella pinodes en-keyword=Pisum sativum kn-keyword=Pisum sativum END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=21 end-page=26 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=FFC Ceramic Water(TM) Enhances Plant Apyrase Activity kn-title=FFC セラミック水(TM)による植物アピラーゼの活性化作用 en-subtitle= kn-subtitle= en-abstract=本報は,FFC セラミックス(TM)(㈱エフエフシー・ジャパン)で調製した FFC セラミック水(FFC 水)の植物アピラーゼ(EC 3.6.1.5)の活性に及ぼす直接的な作用について調べたものである.FFC 水はアピラーゼがもつ ATP加水分解活性を促進し,その作用は反応液への添加量に依存した.先の無機元素分析結果から,FFC 水に含まれる主要な塩類はCa(2+)であることが判明している.そこで,Ca(2+)キレート剤EGTAを反応液へ加え,その影響について調べたところ,FFC 水による活性化作用は消失することが明らかとなった.また,FFC 水と類似の作用は,硫酸カルシウム,塩化カルシウムまたは硝酸カルシウムの添加で認められ,陰イオンの種類によって明確な違いはなかった.これらの結果から,FFC 水が植物アピラーゼに及ぼす活性化作用の一因は,セラミックスから遊離する Ca(2+)に依存しているものと推察された.一方,アピラーゼ活性を指標として,使用済のセラミックスから調製した FFC 水の効果について検討したところ,未使用からの水と比べて,カルシウム濃度ならびに活性化作用の顕著な低下が認められた.このことは,継続的な使用によってセラミックスから遊離する塩類,特にカルシウムの溶出量が大きく変わることを意味し,アピラーゼを用いた本検定が,FFC 水の効果を定量的に確かめる方法の一つとして利用できると考えられた.以上,これらの結果を総合して,FFC水の植物酵素への直接的作用,ならびに植物への施用によって効果が現れる耐病性獲得作用との関連について考察した. kn-abstract=The FFC ceramics(TM) from FFC Japan Co., Ltd. are now widely used in the fields of agriculture, fishery and food industry in Japan. Recently the FFC ceramic beads-based technology has been also applied to meet several environmental problems including pollution in sea, lakes and rivers. In this study the FFC ceramic water was tested for effect on plant enzyme, potato apyrase (EC 3.6.1.5; ATP-diphosphohydrolase), which hydrolyses nucleoside triphosphate (NTP) and -diphosphate (NDP) to produce corresponding nucleoside monophosphate (NMP) and inorganic phosphate (Pi). Addition of the FFC ceramic water to the enzyme reaction mixture markedly enhanced ATP-hydrolyzing activity, when used as ATP as substrate. However, the concomitant presence of Ca(2+) chelator, EGTA (O,O'-bis(2-aminoethyl)ethyleneglycol-N,N,N',N'-tetraacetic acid) with the FFC ceramic water, completely abolished the enzyme activation. In fact, exogenous calcium ion such as CaSO4 mimicked the FFC ceramic water. These results indicate that apyrase activation by the FFC ceramic water largely depends on calcium ions. On the other hand, when the FFC ceramic water prepared from "used" ceramics was tested for the apyrase activity, the enhanced effect on apyrase was decreased compared to the FFC ceramic water from "new" ones. This result, consistent with our present data covering concentration of calcium ions and conductivity, indicates that long and/or successive usage of the ceramic beads results in decrease of contents of released minerals, especially calcium ions. The apyrase-based enzyme assay presented here is probably applicable to estimate and quantify the effect of FFC ceramic water. en-copyright= kn-copyright= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=1 ORCID= en-aut-name=MatsuokaSachiko en-aut-sei=Matsuoka en-aut-mei=Sachiko kn-aut-name=松岡祥子 kn-aut-sei=松岡 kn-aut-mei=祥子 aut-affil-num=2 ORCID= en-aut-name=MeguroAkane en-aut-sei=Meguro en-aut-mei=Akane kn-aut-name=目黒あかね kn-aut-sei=目黒 kn-aut-mei=あかね aut-affil-num=3 ORCID= en-aut-name=HasegawaSachiko en-aut-sei=Hasegawa en-aut-mei=Sachiko kn-aut-name=長谷川幸子 kn-aut-sei=長谷川 kn-aut-mei=幸子 aut-affil-num=4 ORCID= en-aut-name=NishimuraTomio en-aut-sei=Nishimura en-aut-mei=Tomio kn-aut-name=西村富生 kn-aut-sei=西村 kn-aut-mei=富生 aut-affil-num=5 ORCID= en-aut-name=KunohHitoshi en-aut-sei=Kunoh en-aut-mei=Hitoshi kn-aut-name=久能均 kn-aut-sei=久能 kn-aut-mei=均 aut-affil-num=6 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=赤塚植物園生物機能開発研究所 affil-num=4 en-affil= kn-affil=赤塚植物園生物機能開発研究所 affil-num=5 en-affil= kn-affil=赤塚植物園生物機能開発研究所 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=岡山大学 en-keyword=apyrase kn-keyword=apyrase en-keyword=ATP-diphosphohydrolase kn-keyword=ATP-diphosphohydrolase en-keyword=calcium ion kn-keyword=calcium ion en-keyword=FFC ceramic(TM) kn-keyword=FFC ceramic(TM) en-keyword=FFC ceramic water(TM) kn-keyword=FFC ceramic water(TM) END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=13 end-page=20 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=土壌改質材 FFC エースによるオオムギの生育と収量の促進効果 kn-title=Enhancement of Growth and Yield of Barley by the Soil Conditioner FFC-ace en-subtitle= kn-subtitle= en-abstract=本報は,㈱エフエフシー・ジャパンから販売されている土壌改質材FFCエースTMの作物の生長促進効果について,2006年11月から翌年6月,本学農学部内の実験圃場で実施された,オオムギの生育ならびに収量調査に関する試験結果をとりまとめたものである.実施圃場の砂土壌にFFCエースを所定量混和した区画を設け,オオムギの種子を播種した.なお,対照区は非導入土壌とした.定期的に行った生育調査の結果,FFCエースを導入した土壌では非導入の区画と比べて,生育初期における根の生育が良好となり,地上部における分けつ数の増加とともに穂の生長も旺盛となって,1穂当たりの収穫量(粒数)の著しい増加をもたらした.結果,FFCエース導入区における全収量は非導入区と比べて約1.7倍となった.また,それぞれから収穫したオオムギ粒に含まれる栄養価ならびに無機元素類の量には,FFCエースの導入,非導入によって大きな違いは認められず,導入の効果は収量に大きく反映された.事実,調査期間中に行った測定から,FFCエースを投入した土壌で生育するオオムギ葉は高いクロロフィル量を示しており,光合成が促進されているものと考えられた.実際,播種後4ヶ月目以降,光合成ならびに蒸散速度値を測定した結果,FFCエース導入区で生育したオオムギでは常に高い値を示した.また,FFCエースの導入によって強光条件下における水利用効率が促進された.本報告では,FFCエースの投与と空気中からの二酸化炭素の吸収量との関連について考察するとともに,併せて,FFCエースの土壌への導入によって作物の生育に必要な灌水量を大きく減らすことができる可能性についても言及したい. kn-abstract=The effects of a unique soil conditioner, FFC-ace, on photosynthesis, transpiration, growth and yield of barley were examined in a field experiment. FFC-ace well-mixed with sandy soil greatly enhanced root and shoot growth, tillering and the number of grains per stock. The total yield in the treated plot increased by about 172%. The plants grown in the FFC-ace plot were greener and contained a higher level of chlorophyll, compared with the control. Photosynthesis and transpiration, which are tightly linked to productivity were also significantly enhanced at the broad range of photon flux observed in our study. The quality of grain harvested from the FFC-ace plot was similar to the control plot in terms of nutritional and inorganic components. The increased photosynthesis in the FFC-ace treated barley reflects a higher absorption of CO(2) from the atmosphere. It was also noted that the efficiency of water utilization for photosynthesis was significantly greater under the high light intensity in the treated plot. The relationship between application of FFC-ace and absorption of atmospheric CO(2) is discussed. Our investigation provides data showing that application of FFC-ace to soil significantly reduces water requirements for plant growth and yield. en-copyright= kn-copyright= en-aut-name=FujitaKeiko en-aut-sei=Fujita en-aut-mei=Keiko kn-aut-name=藤田景子 kn-aut-sei=藤田 kn-aut-mei=景子 aut-affil-num=1 ORCID= en-aut-name=SuzukiTomoko en-aut-sei=Suzuki en-aut-mei=Tomoko kn-aut-name=鈴木智子 kn-aut-sei=鈴木 kn-aut-mei=智子 aut-affil-num=2 ORCID= en-aut-name=HasegawaSachiko en-aut-sei=Hasegawa en-aut-mei=Sachiko kn-aut-name=長谷川幸子 kn-aut-sei=長谷川 kn-aut-mei=幸子 aut-affil-num=3 ORCID= en-aut-name=MeguroAkane en-aut-sei=Meguro en-aut-mei=Akane kn-aut-name=目黒あかね kn-aut-sei=目黒 kn-aut-mei=あかね aut-affil-num=4 ORCID= en-aut-name=SugiuraHiroyuki en-aut-sei=Sugiura en-aut-mei=Hiroyuki kn-aut-name=杉浦裕幸 kn-aut-sei=杉浦 kn-aut-mei=裕幸 aut-affil-num=5 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=6 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=7 ORCID= en-aut-name=SakaguchiEi en-aut-sei=Sakaguchi en-aut-mei=Ei kn-aut-name=坂口英 kn-aut-sei=坂口 kn-aut-mei=英 aut-affil-num=8 ORCID= en-aut-name=NishimuraTomio en-aut-sei=Nishimura en-aut-mei=Tomio kn-aut-name=西村富生 kn-aut-sei=西村 kn-aut-mei=富生 aut-affil-num=9 ORCID= en-aut-name=KunohHitoshi en-aut-sei=Kunoh en-aut-mei=Hitoshi kn-aut-name=久能均 kn-aut-sei=久能 kn-aut-mei=均 aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=㈱赤塚植物園・生物機能開発研究所 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=岡山大学 affil-num=8 en-affil= kn-affil=岡山大学 affil-num=9 en-affil= kn-affil=㈱赤塚植物園・生物機能開発研究所 affil-num=10 en-affil= kn-affil=岡山大学 en-keyword=barley (Hordeum vulgare L.) kn-keyword=barley (Hordeum vulgare L.) en-keyword=enhanced growth and yield kn-keyword=enhanced growth and yield en-keyword=FFC-ace (soil conditioner) kn-keyword=FFC-ace (soil conditioner) en-keyword=enhanced photosynthesis and transpiration kn-keyword=enhanced photosynthesis and transpiration en-keyword=chemical analysis of grains kn-keyword=chemical analysis of grains END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=1 article-no= start-page=33 end-page=41 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=199702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=バナジン酸によるエンドウ培養細胞におけるフィトアレキシン生産の誘導 kn-title=Orthovanadate Induces Phytoalexin Production in Pea Suspension-Cultured Cells en-subtitle= kn-subtitle= en-abstract=バナジン酸は、エンドウ組織に褐紋病菌エリシターの処理で誘導される一連の防御応答を抑制することが報告されている。そこで、本研究ではバナジン酸のエンドウ培養細胞に対する影響を調べた。バナジン酸は培養細胞から分離した原形質膜画分のポリホスホイノシチド代謝系関連酵素やATPaseの活性を濃度依存的に阻害した。次に、褐紋病菌エリシター、塩化銅(非生物的エリシターの一種)、およびバナジン酸のin vivoでの影響を調べたところ、いずれの単独処理においてもエンドウ培養細胞のピサチン生産を誘導した。褐紋病菌エリシターはエンドウ培養細胞の細胞死(FDAの染色性喪失)を誘導しなかったが、塩化銅、バナジン酸は明らかな毒性を示した。以上の結果とこれまでの知見に基づいて、エンドウ培養細胞に対しては非生物的エリシターとして作用するバナジン酸の作用機構を考察した。 kn-abstract=We previously reported that the addition of orthovanadate suppressed the defense responses of plant differentiated tissues induced by a fungal elicitor.In this report,the effect of orthovanadate on the defense response of pea cultured cells was examined.The activities of ATPase and PI metabolism in plasma membrance fraction,which was prepared from suspension-cultured cells,were inhibited in vitro by orthovanadate as well as those in plasma membrances from pea epicotyl tissues. However,orthovanadate alone induced the accumulation of a phytoalexin, pisatin in suspention-clutured cells of pea in a manner similar to CuCl2.The viability of pea suspension-cultured cells was decreased by orthovanadate as well as by CuCl2.These results indicated that orthovanadate acts as an abiotic elicitor to pea suspension-cultured cell as observed in those of red bean,peanut and Perunia hybrida. en-copyright= kn-copyright= en-aut-name=KawaharaTomoharu en-aut-sei=Kawahara en-aut-mei=Tomoharu kn-aut-name=河原智治 kn-aut-sei=河原 kn-aut-mei=智治 aut-affil-num=1 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=2 ORCID= en-aut-name=KibaAkinori en-aut-sei=Kiba en-aut-mei=Akinori kn-aut-name=木場章範 kn-aut-sei=木場 kn-aut-mei=章範 aut-affil-num=3 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=4 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=5 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Agricultural Science Laboratory, Agricultural Products, Du Pont K. K. affil-num=2 en-affil= kn-affil=Iwate Biotechnology Research Center affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=defense response kn-keyword=defense response en-keyword=elicitor kn-keyword=elicitor en-keyword=Pisum sativum kn-keyword=Pisum sativum en-keyword=suspension-cultured cells kn-keyword=suspension-cultured cells en-keyword=orthovanadate kn-keyword=orthovanadate END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=109 end-page=116 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=病原菌シグナルによるエンドウ原形質膜におけるホスファチジルイノシトールリン脂質のリン酸化とリゾリン脂質生成の制御 kn-title=Phosphorylation of Phosphatidylinositols and Production of Lysophospholipid in Pea Plasma Membrane Are Coordinately Regulated by Elicitor and Suppressor from Mycosphaerella pinodes    en-subtitle= kn-subtitle= en-abstract=エンドウの上胚軸組織により分離した原形質膜画分を褐紋病菌の生産するエリシターで処理すると、ホスファチジルイノシトールリン脂質の急速なリン酸化とリゾリン脂質の生成が誘導されたが、同菌より調製したサプレッサーの共存下では双方とも著しく阻害された。本結果は、ポリホスホイノシチド代謝系と同調的に作動するホリパーゼA活性化が存在すること、さらに、原形質膜における病原菌シグナルの受容・応答には複数の資質代謝系が介在する可能性を示唆している。一方、ホスホリパーゼAの活性化の役割を調べる目的で、本酵素によって原形質膜から生成されると考えられる脂肪酸(リノール酸ならびにリノレン酸)をエンドウ葉に処理したところ、エリシターの非存在下においてもファイトアレキシンであるピサチンの生成が誘導されることが示された。以上から、ポリホスホイノシチド代謝系と同調的に働くホスホリパーゼAがエリシターシグナルの初期伝達に深く関連しているものと考えられた。 kn-abstract=Effects of elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes, on Pl etabolism in pea plasma membrane were examined in vitro. The elicitor induced rapid phosphorylation of phosphatidylinositols as well as production of lysophospholipid in plasma membranes, but these responses were severely inhibited by the suppressor. These results indicate that a membrane-associated phospholipase A is regulated coordinately by fungal signals, together with Pl metabolism, and that it may participate in signal transduction pathways leading to defense responses. To evaluate a possible rote of phospholipase A activation in induction of a pea defense response, the effect of free fatty acid on induction of a phytoalexin accumulation was also examined. When pea leaves were treated with linoleic- or linolenic acid, most commonly released in plant cells by phospholipase A, the accumulation of pisatin was induced even in the absence of the elicitor. It is, therefore, conceivable that free fatty acid(s) released from plasma membrane is also implicated in the early stage of elicitor-signal transduction in pea. en-copyright= kn-copyright= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=1 ORCID= en-aut-name=KoyamaMasashi en-aut-sei=Koyama en-aut-mei=Masashi kn-aut-name=小山昌史 kn-aut-sei=小山 kn-aut-mei=昌史 aut-affil-num=2 ORCID= en-aut-name=MizukoshiRumi en-aut-sei=Mizukoshi en-aut-mei=Rumi kn-aut-name=水越留美 kn-aut-sei=水越 kn-aut-mei=留美 aut-affil-num=3 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=4 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=5 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=elicitor kn-keyword=elicitor en-keyword=pea(Pisum sativum L.) kn-keyword=pea(Pisum sativum L.) en-keyword=phospholipase A kn-keyword=phospholipase A en-keyword=polyphosphoinositide metabolism(Pl metabolism) kn-keyword=polyphosphoinositide metabolism(Pl metabolism) en-keyword=suppressor kn-keyword=suppressor END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=99 end-page=107 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=共生真菌アクレモニウムエンドファイトの形質転換 kn-title=Transformation of Mutualistic Fungal Acremonium Endophyte en-subtitle= kn-subtitle= en-abstract=アクレモニウムエンドファイトの形質転換の条件を検討した。アクレモニウムエンドファイトからプロトプラストを調製し、PEG4000とエレクトロポレーションを用いて形質転換を試みた。その結果、PEGで形質転換すると、エレクトロポレーションによる場合よりも多くの形質転換体が得られた。PCR解析によってiaaM遺伝子のゲノムへの導入を確認したところ、形質転換体のPCR産物はiaaM遺伝子のサイズに相当する約1.7kbのバンドを持っていた。一方、iaaM遺伝子をプローブとしたサザンブロット解析においては、形質転換体と非形質転換体の間にはハイブリダイズした断片数に違いがあることが明らかとなった。以上の結果は、アクレモニウムエンドファイトの形質転換にはPEG法が有効であること、アクレモニウムエンドファイトのゲノム中にはiaaM遺伝子様配列の反復コピーが存在することを示唆している。 kn-abstract=Conditions have been developed for transforming protoplasts of the Acremonium endophyte by PEG 4000 and electroporation. Transformation by PEG exhibited a higher number of transformants than by electroporation. lntegration of iaaM gene into the genome was examined by PCR and Southern blot hybridization analysis. PCR product showed that transformants banded at around 1.7 kb corresponding to the size of iaaM gene. Hybridization of the digests of genomic DNA with iaaM gene as DNA probe showed that the number of hybridized band signals was different between transformant and non-transformant. These results might indicate that PEG is an effective method for the transformation of Acremonium endophyte and that there are repeated copies of the iaaM homologous sequences in the genome of Acremonium. en-copyright= kn-copyright= en-aut-name=YunusAhamad en-aut-sei=Yunus en-aut-mei=Ahamad kn-aut-name=ユーナスアハマド kn-aut-sei=ユーナス kn-aut-mei=アハマド aut-affil-num=1 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=2 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=3 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Acremonium sp kn-keyword=Acremonium sp en-keyword=endophyte kn-keyword=endophyte en-keyword=tranformation kn-keyword=tranformation en-keyword=iaaM gene kn-keyword=iaaM gene en-keyword=hgh gene kn-keyword=hgh gene END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=1 article-no= start-page=91 end-page=97 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウカルコン合成酵素遺伝子PSCHS2のエリシターによる転写活性化に関与するシス制御配列とトランス因子 kn-title=Cis-Regulatory Elements and Trans-acting Factors Involved in the Activation of a Member of Elicitor-Responsive Pea Chalcone Synthase Gene Family, PSCHS2 en-subtitle= kn-subtitle= en-abstract=エンドウのカルコン合成酵素遺伝子,PSCHS2のエリシターによる転写活性化機構を解明するために、PSCHS2プロモーターの転写制御シスエレメントとトランスに働く核内DNA結合タンパク質について解析した。PSCHS2の転写開始点より-471までの配列を有するプロモーターは、高いべーサルな転写活性を示すと共にエリシター処理により転写活性が増高した。また、エリシター処理したエンドウ上胚軸から調製された核抽出物はPSCHS2の+83から-484までの異なるDNA断片とゲルシフトアッセイ移動度の遅い複合体(LMC)を形成した。更に、LMCの形成は核抽出物をアルカリフォスファターゼで処理することにより阻害されたことより、何らかの核タンパク質のリン酸化がLMC形成を促していると考えられた。以上の結果は、PSCHS2のプロモーター上にある複数の転写制御シスエレメントに対する正の転写活性化因子の結合はエリシター処理によって増加し、その結合によって転写が活性化することを示唆している。 kn-abstract=To elucidate the elicitor-mediated transcriptional activation in one of the chalcone synthase genes, PSCHS2 in pea, we investigated the putative cis-regulatory elements in the promoter sequence and trans-acting DNA binding proteins. The promoter up to -471 from the transcription start site of PSCHS2 gave considerable level of basal transcriptional activity. Nuclear extract from elicitortreated pea epicotyls formed DNA-protein complexes with three independent DNA fragments spanning from +83 to -484 of PSCHS2 with low mobility (LMC,low mobility complex) in the gel mobility shift assay. Since the LMC formation was blocked by the treatment of nuclear extract with alkaline phosphatase, the phosphorylation of some nuclear factor(s) assists LMC formation. These results indicate that the bindings of the putative positive nuclear factors to the multiple cisregulatory elements in PSCHS2 promoter region were enhanced by elicitortreatment that might result in transcriptional activation. en-copyright= kn-copyright= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=1 ORCID= en-aut-name=ItoMasayuki en-aut-sei=Ito en-aut-mei=Masayuki kn-aut-name=伊藤正幸 kn-aut-sei=伊藤 kn-aut-mei=正幸 aut-affil-num=2 ORCID= en-aut-name=SekiHikaru en-aut-sei=Seki en-aut-mei=Hikaru kn-aut-name=関光 kn-aut-sei=関 kn-aut-mei=光 aut-affil-num=3 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=4 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=5 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=chalcone synthase kn-keyword=chalcone synthase en-keyword=cis-acting elements kn-keyword=cis-acting elements en-keyword=DNA binding proteins kn-keyword=DNA binding proteins en-keyword=elicitor kn-keyword=elicitor en-keyword=promoter activity kn-keyword=promoter activity END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=31 end-page=38 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウ原形質膜におけるATPアーゼとホスファチジルイノシトールリン資質リン酸化酵素の共精製 kn-title=Co-purification of Plasma Membrane ATPase and Phosphatidylinositol Kinase from Pea Plasma Membranes en-subtitle= kn-subtitle= en-abstract=エンドウの上胚軸組織から分離した原形質膜画分におけるATPアーゼとホスファチジルイノシトールリン脂質リン酸化酵素との相互作用を解析する目的で、双方の原形質膜画分からの可溶化とそれらの部分精製を試みた。原形質膜のTritonX-100可溶化画分をグリセロール連続密度勾配遠心分画に供し、得られた活性画分をさらに分子ふるいカラムクロマトグラフィーによって分離した。この結果、ATPアーゼとホスファチジルイイノシトールリン脂質リン酸化酵素は共精製され、非変性条件下では双方の活性を分けることができなかった。 kn-abstract=The plasma membrance ATPase was partially purified by a linear glycerol density gradient centrifugation of the detergent-solubilized plasma membrance poteins and subsequent separation by a size-exclusion column chromatogrphy. A purified ATPase preparation is shown to contain a 97.6kDa protein that was cross-reacted with an antibody raised against mung bean H+-ATPase. The preparation also exhibited the phosphorylation of exogenous phosphatidylionsitol(PI) when supplized with [γ-32P]ATP. These results indicate that one form plasma membrance ATPase is co-purified with PI kinase. en-copyright= kn-copyright= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=1 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=2 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=3 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Lipid kinase kn-keyword=Lipid kinase en-keyword=Mycosphaerella kn-keyword=Mycosphaerella en-keyword=plasma membrance ATPase kn-keyword=plasma membrance ATPase en-keyword=pea(Pisum sativum L.) kn-keyword=pea(Pisum sativum L.) en-keyword=suppressor kn-keyword=suppressor END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=25 end-page=30 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウの推定ZnフィンガーDNA結合性タンパク質のcDNAクローニング kn-title=Molecular Cloning of a cDNA Encoding a Putative DNA-Binding Zinc-Finger Protein in Pea en-subtitle= kn-subtitle= en-abstract=エリシターを処理したエンドウの上胚軸からcDNAライブラリーを作成し、水処理上胚軸をコントロールに differential screening を行い、エリシター応答性cDNAの候補として90個のクローンを単離した。そららのクローンの1つE31は1,716bpのインサートを有し、ミヤコグサ、ダイズでcDNAがクローニングされているLjpzf、Gmpzfのエンドウにおけるホモローグをコードしていると考えられ、その推定翻訳産物をPspzfと命名した。E31は、Ljpzfの推定アミノ酸配列の82アミノ酸目からカルボキシル末端側をコードしていると考えられた。LjpzfやPspzfの推定アミノ酸配列中には核移行シグナルHKRKが存在していたこと、カルボキシル末端側にはCys3His2Cys3もモチーフとするRing H2 fingerドメインが存在していたことより、LjpzfやPspzfは、核内に局在するDNA結合性のZn fingerタンパク質の1種として遺伝子発現の制御に機能している可能性が示唆された。 kn-abstract=We constructed cDNA library from pea epicotyls treated with fungal elicitor for 5hrs, and performed differential screening using the individual 32P-cDNA probe derived from poly(A)+ RNA prepared from elicitor- and water-treated epicotyls. As a result of the screening, we have isolated from elicitor- and water-treated epicotyls. As a results of the screening, we have isolated about 90 cDNA clones as candidates for elicitor-inducible genes, and their nucleotide sequences have been partially determined. One of these clones, E31 was a pea homolog of the putative zinc-finger proteins, Ljpzf in Lotus japonicus and Gmpz in soybean. E31 possesses 1,726bp insert, and encodes an open reading frame corresponding to position 82 amino acids from N-terminus to the C-terminal end in Ljpzf. The protein product of E31 was designated as Pspzf. Pspzf also possesses nuclear localization signal(NLS), HKRK, and Cys3His2Cys3(RIngH2) motif at the same position to LjpZF and putative C-terminal end of the deduced amino acid sequences, respectively. Since zinc-finger motif is one of the well-known DNA-binding domains, Ljpzf and Pspzf might be able to bins to a particular DNA sequence and regulate transcriptional activity in plants. en-copyright= kn-copyright= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=1 ORCID= en-aut-name=EndohAi en-aut-sei=Endoh en-aut-mei=Ai kn-aut-name=遠藤愛 kn-aut-sei=遠藤 kn-aut-mei=愛 aut-affil-num=2 ORCID= en-aut-name=SanematsuShiroh en-aut-sei=Sanematsu en-aut-mei=Shiroh kn-aut-name=実松史郎 kn-aut-sei=実松 kn-aut-mei=史郎 aut-affil-num=3 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=4 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=5 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=DNA-binding protein kn-keyword=DNA-binding protein en-keyword=Elicitor kn-keyword=Elicitor en-keyword=Ring H2 finger protein kn-keyword=Ring H2 finger protein en-keyword=Transcription factors kn-keyword=Transcription factors en-keyword=Zinc-finger protein kn-keyword=Zinc-finger protein END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=1 article-no= start-page=21 end-page=26 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウのエリシター誘導性遺伝子発現におけるAAAGモチーフとPsDof1タンパク質の関与 kn-title=Possible Involvement of AAAG Motif and PsDof1 in Elicitor-Induced Gene Expression in Pea en-subtitle= kn-subtitle= en-abstract=エリシターを処理したエンドウ上胚軸由来のRNAから作成されたcDNAライブラリーからエリシター処理により発現が増高する遺伝子候補のcDNAとしてPsDof1が単離された.大腸菌で生産されたGST-PsDof1融合タンパク質はAAAG配列をコアとするDNAに結合することが明らかにされている.本論文ではGST-PsDof1がエリシター応答性遺伝子の一つ,PsCHS1のプロモーター上のAAAGまたはCTTT配列を有する断片に特異的に結合することを明らかにした.更にAAAG配列のエリシター応答性シスエレメントとしての機能を解析するため,AAAG配列を4回繰り返したユニットをCaMV35Sの最小プロモーターとCATレポーター遺伝子に連結したキメラ遺伝子を構築し,エンドウプロトプラストにエレクトロポレーション法により導入した.CAT活性を指にプロモーター活性を調べたところ,AAAG配列を有するプロモーターは,エリシター処理により活性化されることが明らかとなった.これらの結果はPsDof1がエリシター応答性防御遺伝子のプロモーター上のAAAG配列に結合し,転写を活性化させる可能性を示唆している. kn-abstract=Recently, we, isolated cDNA clone, PsDof1, from clicitor-treated pea cDNA library. The putative gene product, a PsDof1, encodes DNA binding protein that specifically binds the DNA fragment containing AAAG core sequence. In this paper we report that GST-PsDof1 fusion protein specifically binds to the promoter region containing AAAG core sequence(s) of PsCHS1, one of the elicitor-inducible genes encoding chalcone synthase (CHS). Furthermore the addition of DNA fragment containing AAAG motif to the 35S minimal promoter provided the elicitor-responsibility in transient transfection assay using pea protoplasts. These results suggest that PsDof1 might be involved in defense responses by acitivating the transcription by a binding to AAAG core sequence in the promoter of the defense-related genes in pea. en-copyright= kn-copyright= en-aut-name=SekiHikaru en-aut-sei=Seki en-aut-mei=Hikaru kn-aut-name=關光 kn-aut-sei=關 kn-aut-mei=光 aut-affil-num=1 ORCID= en-aut-name=MarutaniMizuri en-aut-sei=Marutani en-aut-mei=Mizuri kn-aut-name=丸谷瑞理 kn-aut-sei=丸谷 kn-aut-mei=瑞理 aut-affil-num=2 ORCID= en-aut-name=InagakiYoshishige en-aut-sei=Inagaki en-aut-mei=Yoshishige kn-aut-name=稲垣善茂 kn-aut-sei=稲垣 kn-aut-mei=善茂 aut-affil-num=3 ORCID= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=4 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=5 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=cis-element kn-keyword=cis-element en-keyword=DNA binding proteins kn-keyword=DNA binding proteins en-keyword=Dof protein kn-keyword=Dof protein en-keyword=Elicitor kn-keyword=Elicitor END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=1 article-no= start-page=51 end-page=60 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウプロトプラスト細胞における植物プロモータの発現 kn-title=A Reporter Gene Expression Under the Control of a Pea Phenylalanine Ammonia Lyase-gene Promoter en-subtitle= kn-subtitle= en-abstract= kn-abstract=High yields of viable pea protoplasts were produced from suspension cultured cells derived from calli formed from embryogenic tissues or leaves and the conditions for the optimum expression of chloramphenicol acetyltransferase (CAT) fused to the phenylalanine ammonia-lyase gene of Pisum sativum (pPAL1-15) were investigated by transient assay after electroporation. A fungal elicitor isolated from a pea pathogen, Mycosphaerella pinodes, and the reduced from of glutathione induced the expression of PAL promoter but orthovanadate, a plasma membrane ATPase inhibitor, considerably suppressed the gene expression. Rice protoplasts were also prepared from the suspension cultured cells derived from embryonic tissues, and the effects of elicitors on the expression of CAT in pPAL1-15-electroporated rice protoplasts were examined. No distinctive induction of CAT activity was observed by the treatment of rice protoplasts with a chitosan oligomer elicitor. en-copyright= kn-copyright= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=1 ORCID= en-aut-name=HashimotoTadaaki en-aut-sei=Hashimoto en-aut-mei=Tadaaki kn-aut-name=橋本忠明 kn-aut-sei=橋本 kn-aut-mei=忠明 aut-affil-num=2 ORCID= en-aut-name=SriprasertsakPermpong en-aut-sei=Sriprasertsak en-aut-mei=Permpong kn-aut-name=スリプラサートサックペルンポン kn-aut-sei=スリプラサートサック kn-aut-mei=ペルンポン aut-affil-num=3 ORCID= en-aut-name=KatoHisaharu en-aut-sei=Kato en-aut-mei=Hisaharu kn-aut-name=加藤久晴 kn-aut-sei=加藤 kn-aut-mei=久晴 aut-affil-num=4 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=5 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=6 ORCID= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=1 article-no= start-page=1 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=1982 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウ褐紋病菌の胞子発芽液に含まれるピサチン蓄積誘導物質の分離と性質 kn-title=Isolation and Partial Characterization of an Elicitor of Pisatin Production from Spore Germination Fluid of Pea Pathogen, Mycosphaerella pinodes en-subtitle= kn-subtitle= en-abstract= kn-abstract=An elicitor of pisatin accumulation was isolated and partially characterized from spore germination fluid, mycelial extract, and cell wall preparation of a pea pathogen, Mycosphaerella pinodes. The elicitor was found to be a polysaccharide or glycoprotein and the active component was composed of glucose. The approximate molecular weight was 70,000 daltons. This elicitor elicited pisatin accumulation in pea leaves at a concentration of 10 ug/ml and the maximum activity was ca. 350-400 ug/ml, but did not elicit phytoalexins in soybean, bean, and red clover. High molecular weight preparations from the mycelial extract and mycelial wall of Mycosphaerella melonis and Stemphylium sarcinaeforme, nonpathogens of pea, elicited pisatin accumulation in pea leaves. en-copyright= kn-copyright= en-aut-name=ThanutongPorntip en-aut-sei=Thanutong en-aut-mei=Porntip kn-aut-name=タヌートンポーンチップ kn-aut-sei=タヌートン kn-aut-mei=ポーンチップ aut-affil-num=1 ORCID= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=2 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=3 ORCID= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=1 article-no= start-page=1 end-page=8 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=1979 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=殺菌剤、ベノミル、チウラムの土壌微生物相と2~3の土壌生息性糸状菌に及ぼす影響 kn-title=Effect of Fungicides, Benomyl and Thiram on Soil Microflora and Some Inhabitant Fungi en-subtitle= kn-subtitle= en-abstract= kn-abstract=Effects of Benomyl and Thiram on the soil microflora and some soil inhabitant fungi were studied using the_ soil (sandy loam) of the experimental field, Okayama University. Under field conditions, heavy application of Benomyl did not affect significantly the soil microflora. Thiram, however, reduced the fungal population in soil to 1/6 at the next day of treatment, but recovered to the normal level after 6 days. Under laboratory conditions, both fungicides did not affect soil microflora. Population of Benomyl-to lerant fungi was about 1/10 of the total fungi and increased slightly in the field soil by treatment with Benomyl at the later stage of experiment during June to October. Neither Thiram-tolerant fungus nor bacterium was found in both Thiram-treated and non-treated soils. A fungus highly tolerant to Benomyl was isolated and identified as Aspergillus versicolor, and found to not have the metabolic activity to degrade BCM. The absorption of BCM by the mycelia of this tolerant fungus, A. versicolor, was less than half of the BCM-sensitive one, such as Cladosporium harbarum. en-copyright= kn-copyright= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=1 ORCID= en-aut-name=OkiKazuo en-aut-sei=Oki en-aut-mei=Kazuo kn-aut-name=沖和生 kn-aut-sei=沖 kn-aut-mei=和生 aut-affil-num=2 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=3 ORCID= en-aut-name=SatoKenji en-aut-sei=Sato en-aut-mei=Kenji kn-aut-name=佐藤健二 kn-aut-sei=佐藤 kn-aut-mei=健二 aut-affil-num=4 ORCID= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=Okayama Agricultural Experiment Station affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=1977 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Control Measure of Brown Spot of Grapes kn-title=ブドウ褐点病の防除について en-subtitle= kn-subtitle= en-abstract= kn-abstract=ブドウ褐点病防除のための農薬のスクリーニングを行なうとともに,発生生態の観察結果を含めて本病の防除施策について論じた. 供用農薬のなかでは,ベンレート,サニパー,DF125が有効であった. 自然発生,特に汚果の防止には硫酸ニコチンの混用が有効であった. 外気の直接導入を避けるような温室出入口の構造改善,室内浄化,誘枝線の更新,過湿の回避などが有効な技術的な防除策と考えられた。 en-copyright= kn-copyright= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=1 ORCID= en-aut-name=HatamotoMotomu en-aut-sei=Hatamoto en-aut-mei=Motomu kn-aut-name=畑本求 kn-aut-sei=畑本 kn-aut-mei=求 aut-affil-num=2 ORCID= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=3 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=4 ORCID= en-aut-name=TateishiMichihiro en-aut-sei=Tateishi en-aut-mei=Michihiro kn-aut-name=立石道博 kn-aut-sei=立石 kn-aut-mei=道博 aut-affil-num=5 ORCID= en-aut-name=FujiiShintaro en-aut-sei=Fujii en-aut-mei=Shintaro kn-aut-name=藤井新太郎 kn-aut-sei=藤井 kn-aut-mei=新太郎 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山県農業試験場 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=島根県農業試験場 affil-num=6 en-affil= kn-affil=岡山県農業試験場 END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=1 article-no= start-page=17 end-page=22 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=1976 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=クラドスポリウム属菌によるブドウの褐点病について kn-title=Brown Spot of Grapes Caused by Cladosporium cladosporioides and Cladosporium herbarum en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cladosporium cladosporioides (Fresenius) de Vries and Cladosporium herbarum (Pers. ) Link ex Fr. were isolated from grape berries that had been commonly called 'black navel', and were found to cause the disease. In view of the colour of lesions that were observed most frequently at the stage of maturing, the disease was named brown spot of grapes. en-copyright= kn-copyright= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=1 ORCID= en-aut-name=HatamotoMotomu en-aut-sei=Hatamoto en-aut-mei=Motomu kn-aut-name=畑本求 kn-aut-sei=畑本 kn-aut-mei=求 aut-affil-num=2 ORCID= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=3 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=4 ORCID= en-aut-name=YokoyamaTatsuo en-aut-sei=Yokoyama en-aut-mei=Tatsuo kn-aut-name=横山竜夫 kn-aut-sei=横山 kn-aut-mei=竜夫 aut-affil-num=5 ORCID= en-aut-name=TateishiMichihiro en-aut-sei=Tateishi en-aut-mei=Michihiro kn-aut-name=立石道博 kn-aut-sei=立石 kn-aut-mei=道博 aut-affil-num=6 ORCID= en-aut-name=FujiiShintaro en-aut-sei=Fujii en-aut-mei=Shintaro kn-aut-name=藤井新太郎 kn-aut-sei=藤井 kn-aut-mei=新太郎 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=Okayama Agricultural Experiment Station affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=Institute for Fermentation affil-num=6 en-affil= kn-affil=Extension Service, Shimane Agricultural Experiment Station affil-num=7 en-affil= kn-affil=Okayama Agricultural Experiment Station END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1 article-no= start-page=21 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=1976 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Chitin Component in Haustorial Wall of Powdery Mildew Fungus of Barley kn-title=オオムギうどんこ病菌吸器細胞壁におけるキチン成分について en-subtitle= kn-subtitle= en-abstract= kn-abstract=純寄生性病害の感染成立に中心的役割を演ずると考えられている吸器の性質を明らかにするために,オオムギうどんこ病菌の吸器形成過程における吸器壁のキチン質の生成に関して組織化学的に検討した. その結果,吸器壁はキトーサン反応陽性で,菌糸壁と同様キチン質がその骨格をなしているものと考えられる. さらに,経時的な検討の結果,感染初期に形成される吸器原基において,すでにキチン反応が陽性であり,キチン合成系の活性化は,吸器機能発現のための一つの重要な過程であると推定される。 en-copyright= kn-copyright= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=1 ORCID= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=2 ORCID= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=47 cd-vols= no-issue=1 article-no= start-page=7 end-page=13 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=1976 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=土壌菌による有機塩素殺菌剤、ダコニールの吸収と脱クロールについて kn-title=Uptake and Dechlorination of an Organochlorine Fungicide, Tetrachloroisophthalonitrile, Daconil, by Some Soil Fungi en-subtitle= kn-subtitle= en-abstract= kn-abstract=Fungi tolerant to an organochlorine fungicide, tetrachloroisophthalonitrile (TCPN), was selected and isolated from various soil samples, and examined for the activity to metabolize TCPN. The population of tolerant fungi was extremely large in the plastic house - soil on which TCPN has been used for six years to control diseases. This is probably due to the change of soil microflora owing to the decline of TCPN-sensitive competitors. Many tolerant fungi took up TCPN very rapidly from culture filtrate. Of ten tolerant isolates tested, two isolates, C-F- I and V-P-5, were found to metabolize TCPN. The metabolite was isolated in pure form by preparative TLC, and identified as trichloroisophthalonitrile by mass spectrometry. C-F-1 was identified as Aspergillus luchuensis and V-P-5 as Penicillium godlewskii. Namely, these soil fungi dechlorinate from tetrachloroisophthalontrile to trichloroisophthalonitrile. en-copyright= kn-copyright= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=1 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=2 ORCID= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=3 ORCID= en-aut-name=HoriuchiSayoko en-aut-sei=Horiuchi en-aut-mei=Sayoko kn-aut-name=堀内佐代子 kn-aut-sei=堀内 kn-aut-mei=佐代子 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=1 article-no= start-page=17 end-page=20 dt-received= dt-revised= dt-accepted= dt-pub-year=1973 dt-pub=1973 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=農業用殺菌剤、植物病原菌の有害代謝産物によるファイトアレキシンの生産 kn-title=Phytoalexin Induction by Some Agricutural Fungicides and Phytotoxic Metabolites of Pathogenic Fungi en-subtitle= kn-subtitle= en-abstract= kn-abstract=The endocarp of the fresh pea pod incubated with solution or suspension of agricultural fungicides, phytotoxic metabolites of plant pathogenic fungi formed the pea phytoalexin, pisatin. Among the compounds tested, cycloheximide, triazine, dichlone, phenyimercury acetate, UV degradation product of phenylmercury acetate, triphenyltin fungicides, and 3-hydroxy-5-methylisoxazole induced pisatin. Ophiobolin, a toxin from Cochliobolus mtyabeanus, and ascochitine, a toxic metabolite from Ascochyta fabae, also induced pisatin. The possibilities of the development of harmless plant disease control agents was discussed in relation- to the induced synthesis of phytoalexins. en-copyright= kn-copyright= en-aut-name=OkuHachiro en-aut-sei=Oku en-aut-mei=Hachiro kn-aut-name=奥八郎 kn-aut-sei=奥 kn-aut-mei=八郎 aut-affil-num=1 ORCID= en-aut-name=NakanishiToshiro en-aut-sei=Nakanishi en-aut-mei=Toshiro kn-aut-name=中西逸朗 kn-aut-sei=中西 kn-aut-mei=逸朗 aut-affil-num=2 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=3 ORCID= en-aut-name=OuchiSeiji en-aut-sei=Ouchi en-aut-mei=Seiji kn-aut-name=大内成志 kn-aut-sei=大内 kn-aut-mei=成志 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=Agricultural Chemicals Research Laboratories, Sanyo Co., Ltd. affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 END