ID | 62342 |
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Khan, Haris Ahmed
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)
Sato, Yukiyo
Institute of Plant Science and Resources, Okayama University
Kondo, Hideki
Institute of Plant Science and Resources, Okayama University
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Jamal, Atif
Crop Diseases Research Institute, National Agricultural Research Centre
Bhatti, Muhammad Faraz
Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)
Suzuki, Nobuhiro
Institute of Plant Science and Resources, Okayama University
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Abstract | A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum. Here, we describe the molecular and biological characterisation of a second hadakavirus strain from Fusarium nygamai, which has not been investigated in detail previously as a virus host. This virus, hadakavirus 1 strain 1NL (HadV1-1NL), has features similar to the first hadakavirus, HadV1-7n, despite having a different number of segments (10 for HadV1-1NL vs. 11 for HadV1-7n). The 10 genomic RNA segments of HadV1-1NL range in size from 0.9 kb to 2.5 kb. All HadV1-1NL segments show 67% to 86% local nucleotide sequence identity to their HadV1-7n counterparts, whereas HadV1-1NL has no homolog of HadV1-7n RNA8, which encodes a zinc-finger motif. Another interesting feature is the possible coding incapability of HadV1-1NL RNA10. HadV1-1NL was predicted to be capsidless based on the RNase A susceptibility of its replicative form dsRNA. Phenotypic comparison of multiple virus-infected and virus-free single-spore isolates indicated asymptomatic infection by HadV1-1NL. Less-efficient vertical transmission via spores was observed as the infected fungal colonies from which the spores were derived became older, as was observed for HadV1-7n. This study shows a second example of a hadakavirus that appears to have unusual features.
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Note | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00705-021-05176-x
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Published Date | 2021-7-27
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Publication Title |
Archives of Virology
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Volume | volume166
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Publisher | Springer Science and Business Media LLC
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Start Page | 2711
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End Page | 2722
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ISSN | 0304-8608
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2021 Springer Nature Switzerland AG
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File Version | author
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Related Url | isVersionOf https://doi.org/10.1007/s00705-021-05176-x
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
Japan Society for the Promotion of Science
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助成番号 | 17H01463
16H06436
16H06429
16K21723
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