Author | Matsuura, Hiroko| Murakami, Takashi| Hina, Kazuyoshi| Yamamoto, Keizo| Kawamura, Hiroshi| Sogo, Taiji| Shinohata, Ryoko| Usui, Shinichi| Ninomiya, Yoshifumi| Kusachi, Shozo| |
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Published Date | 2008-02 |
Publication Title | Clinical Biochemistry |
Volume | volume41 |
Issue | issue3 |
Content Type | Journal Article |
JaLCDOI | 10.18926/AMO/31831 |
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FullText URL | fulltext.pdf |
Author | Hatipoglu, Omer Faruk| Hirohata, Satoshi| Yaykasli, Kursat Oguz| Cilek, Mehmet Zeynel| Demircan, Kadir| Shinohata, Ryoko| Yonezawa, Tomoko| Oohashi, Toshitaka| Kusachi, Shozo| Ninomiya, Yoshifumi| |
Abstract | ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA. |
Keywords | ADAMTS1 gene regulation metalloproteinase |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2009-04 |
Volume | volume63 |
Issue | issue2 |
Publisher | Okayama University Medical School |
Start Page | 79 |
End Page | 85 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
File Version | publisher |
Refereed | True |
PubMed ID | 19404339 |
Web of Science KeyUT | 000265457600002 |
Author | Shinohata, Ayako| |
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Published Date | 2008-09-30 |
Publication Title | |
Content Type | Thesis or Dissertation |