ID | 49094 |
FullText URL | |
Author |
Mayer, Klaus F. X.
Martis, Mihaela
Hedley, Pete E.
Simkova, Hana
Liu, Hui
Morris, Jenny A.
Steuernagel, Burkhard
Taudien, Stefan
Roessner, Stephan
Gundlach, Heidrun
Kubalakova, Marie
Suchankova, Pavla
Murat, Florent
Felder, Marius
Nussbaumer, Thomas
Graner, Andreas
Salse, Jerome
Endo, Takashi
Sakai, Hiroaki
Tanaka, Tsuyoshi
Itoh, Takeshi
Platzer, Matthias
Matsumoto, Takashi
Scholz, Uwe
Dolezel, Jaroslav
Waugh, Robbie
Stein, Nils
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Abstract | We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign; similar to 86% of the estimated; similar to 32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
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Published Date | 2011-04
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Publication Title |
The Plant Cell
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Volume | volume23
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Issue | issue4
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Start Page | 1249
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End Page | 1263
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ISSN | 1040-4651
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Content Type |
Journal Article
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Official Url | http://dx.doi.org/10.1105/tpc.110.082537
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language |
English
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Copyright Holders | © 2011 American Society of Plant Biologists
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File Version | publisher
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Refereed |
True
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DOI | |
Web of Science KeyUT |