JaLCDOI |
10.18926/15363
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FullText URL |
Mem_Fac_Eng_OU_34_39.pdf
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Author |
Hayakawa, Satoshi|
Shirosaki, Yuki|
Yabuta, Takeshi|
Tsuru, Kanji|
Ohtsuki Chikara|
Osaka, Akiyoshi|
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Abstract |
Silicone elastomer was chemically treated at 60℃ for 7 days with 30 wt% H(2)O(2) solutions with or without TaCl(5) and soaked for various periods in a simulated body
fluid(Kokubo solution) up to 21 days. Apatite formation ability of the surface of the silicone elastomer specimens was investigated with thin-film X-ray diffraction and FT-IR
reflection spectroscopy. These silicone specimens did not deposit apatite or calcium phosphates, irrespective of chemical treatment. Osteoblast-like cells (MC3T3-El) derived from mouse were cultured on the specimens at 36.5℃ under 5%C0(2) and 95% humidity. Similar degree of proliferation of cells was observed at 7 days among three specimens, while the no treatment specimen after incubation for 5 days showed a lower degree of proliferation than the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). Alkaline phosphatase activity of the cells proliferated on the no treatment specimen was lower than those of the silicone treated with 30 wt% H(2)O(2) solutions with or without
TaCl(5). These results indicate that the cytotoxicity of the silicone could be improved by the chemical treatment with 30 wt% H(2)O(2) solutions with or without TaCl(5).
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Publication Title |
Memoirs of the Faculty of Engineering, Okayama University
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Published Date |
2000-03-27
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Volume |
volume34
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Issue |
issue1-2
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Start Page |
39
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End Page |
43
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ISSN |
0475-0071
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language |
English
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File Version |
publisher
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NAID |
120002308019
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