| FullText URL | fulltext.pdf |
|---|---|
| Author | Tanaka, Keisuke| Sasaki, Ken| Matsumura, Kentarou| Yajima, Shunsuke| Miyatake, Takahisa| |
| Published Date | 2021-11-08 |
| Publication Title | Scientific Reports |
| Volume | volume11 |
| Issue | issue1 |
| Publisher | Nature Portfolio |
| Start Page | 21816 |
| ISSN | 2045-2322 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2021 |
| File Version | publisher |
| PubMed ID | 34750398 |
| DOI | 10.1038/s41598-021-00987-z |
| Web of Science KeyUT | 000716101700097 |
| Related Url | isVersionOf https://doi.org/10.1038/s41598-021-00987-z |
| JaLCDOI | 10.18926/11778 |
|---|---|
| Title Alternative | Determination of Hematinic Acid Produced by Oxidative Cleavage of Hemoglobin Heme in Red Blood Cells |
| FullText URL | 001_077_082.pdf |
| Author | Hirota, Kazuhiro| Sasaki, Kenji| Hirota, Takashi| |
| Abstract | Our previous studies on the mechanism of phenylhydrazine-induced hemolytic anemia have shown that hematinic acid, one of oxidative cleavage products of heme, is formed by the reaction of hemoglobin with phenylhydrazine. Develoment of the determination of hematinic acid formed by this reaction in red blood cells (RBC) was required to study the mechanism of the hemolysis. Hemolysates prepared by lysis of fresh human RBC with water was mixed with standard hematinic acid. A solution consisting of hydrochloric acid, methanol, and acetone was added, and most of hemoglobin precipitated was removed by centrifugation. Hematinic acid was derived to the methyl ester by incubation with methanol containing sulfuric acid. The ester was passed to two type of silica gel column to remove interferences, and was analysed on a reversedphase high-performance liquid chromatographic column. Hematinic acid could be determined in the range 1.0-20.0μmol/ml RBC. Recovery from hemolysate was 65.0% ±3.5%. Standard compounds of hematinic acid and its methyl ester were prepared by the oxidation of hemin with hydrogen peroxide, and were comfirmed by elemental analyses and mass spectra. |
| Keywords | hematinic acid high-performance liquid chromatography red blood cells heme |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 77 |
| End Page | 82 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313412 |
| JaLCDOI | 10.18926/11773 |
|---|---|
| FullText URL | 001_069_076.pdf |
| Author | Sasaki Kenji| Hirota Takashi| Iwado Akimasa| Hirota Kazuhiro| |
| Abstract | Reactions of cyclic ketones such as α-tetralone, 1,3-cyclohexanedione, or naphthalenedione with formamide or trisformylaminomethane (TFAM) have shown to form polyclic fused pyrimidines by us. Reactions of terpene ketones like l-menthone, d-camphor, l-carvone with TFAM were performed, and 8-isopropyl-5-methyl-5,6,7,8-tetrahydroquinazoline, borno[2,3-d] pyrimidine, and 5-isopropenyl-8-methyl-5,6-dihydroquinazoline were expectedly obtained from three terpenes. Minor products of 5-isopropenyl-8-methyl-5,6,7,8-tetrahydroquinazoline and 5-isopropenyl-8-methylquinazoline were formed with 5-isopropenyl-8-methyl-5,6-dihydroquinazoline by disproportionation reaction of l-carvone. Furthermore, No-formylmenthylamine, N-formylbornylamine, and N-formylcarvylamine were obtained as the Leuckart-type products terpene ketones in these reactions. The reaction of N-benzyl-4-piperidone with TFAM gave desired 6-benzyl-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine. The reaction of diethyl succinylsuccinate with TFAM afforded tricyclic 4,9-dioxo-3,4,8,9-tetrahydropyrimido[4,5-g]quinazoline. Above compounds were determined by the measurements of their instrumental analyses. |
| Keywords | Cyclic ketone Trisformylaminomethane Fused pyrimidine Cyclization Reductive amination |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 69 |
| End Page | 76 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313993 |
