We examined the effect of fetal calf serum (FCS) on meiotic division, subsequent fertilization, and first cleavage to the 2-cell stage of rat oocytes during in vitro maturation. FCS had no effect on the nuclear progression from dictiate to metaphase of the second maturation in vitro and, FCS had no effect on the first cleavage to the 2-cell stage of fertilized oocytes. However, FCS efficiently increased penetration rate of oocytes and shortened the time required for dissolution of the zona pellucida by alpha-chymotrypsin. These results showed that FCS did not affect cytoplasmic maturation necessary for oocytes to develop to the 2-cell stages. We found that FCS only affects the zona pellucida and does not affect the nucleus or cytoplasm of rat oocytes. FCS may prevent hardening of the zona pellucida.
en-copyright= kn-copyright= en-aut-name=FujiiYoshitaka en-aut-sei=Fujii en-aut-mei=Yoshitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaTamotsu en-aut-sei=Yoshioka en-aut-mei=Tamotsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Center for Adult Diseases en-keyword=in vitro fertilization kn-keyword=in vitro fertilization en-keyword=in vitro maturation kn-keyword=in vitro maturation en-keyword=fetal calf serum kn-keyword=fetal calf serum en-keyword=rat kn-keyword=rat en-keyword=zona pellucida kn-keyword=zona pellucida END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=2 article-no= start-page=103 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in the levels of lipoperoxide and antioxidant factors in human placenta during gestation. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The concentration of lipoperoxides in maternal blood increases as gestation progresses. The concentration in pregnant women at 40 weeks gestation is 1.6 times higher than in nonpregnant women. The concentration in the cord blood, however, is 70% lower than that in maternal blood. To study the role of placental tissue in the difference in the lipoperoxide concentration between the cord blood and maternal blood, we investigated the lipoperoxide concentration, antioxidant activities and in vitro lipoperoxide formation in placental tissue during pregnancy. The lipoperoxide concentration was 50% lower in placental tissue of 40 weeks gestation than in tissue of 5-11 weeks gestation. Catalase and superoxide dismutase activities in placental tissues increased as gestation progressed, while glutathione peroxidase activity and alpha-tocopherol concentration did not change significantly during the gestational period. The in vitro formation of lipoperoxides in placental tissue decreased as gestation progressed. These results show that placental tissue suppresses lipoperoxide formation in the late gestational age, lowers the concentration of lipoperoxides in the blood and protects the fetus against oxygen toxicity.
en-copyright= kn-copyright= en-aut-name=TakeharaYoshiki en-aut-sei=Takehara en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshiokaTamotsu en-aut-sei=Yoshioka en-aut-mei=Tamotsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Center for Adult Diseases affil-num=2 en-affil= kn-affil=Center for Adult Diseases affil-num=3 en-affil= kn-affil=Okayama University en-keyword=lipoperoxides kn-keyword=lipoperoxides en-keyword=antioxidant factors kn-keyword=antioxidant factors en-keyword=placenta kn-keyword=placenta en-keyword=human kn-keyword=human en-keyword=gestation kn-keyword=gestation END start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue=4 article-no= start-page=307 end-page=312 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=198208 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cationized ferritin-induced cap formation and the effect of cytochalasin B. en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ImanakaMasaaki en-aut-sei=Imanaka en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriMasaharu en-aut-sei=Mori en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamotoShu en-aut-sei=Nakamoto en-aut-mei=Shu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KirizukaKeizi en-aut-sei=Kirizuka en-aut-mei=Keizi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OtsukaNagayasu en-aut-sei=Otsuka en-aut-mei=Nagayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=cytochalasin B kn-keyword=cytochalasin B en-keyword=Ehrlich ascites tumor cells kn-keyword=Ehrlich ascites tumor cells en-keyword=cappong kn-keyword=cappong en-keyword=zeiosis kn-keyword=zeiosis en-keyword=cationized ferritin kn-keyword=cationized ferritin END start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue=6 article-no= start-page=483 end-page=486 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=198212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title="Pseudo-cap" formation in Ehrlich ascites tumor cells induced by cytochalasin B. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cytochalasin B (CB) treatment induces or accelerates the capping phenomenon in some cells. In Ehrlich ascites tumor cells (EATC) CB treatment apparently induced the capping of Con A binding sites as observed under a fluorescent microscope. However, electron microscopic examinations revealed that the CB treatment did not induce a rearrangement of Con A binding sites, but rather it only induced a change in cell shape. On the contrary, CB treatment inhibited the capping phenomenon induced by treatment with Con A. Electron microscopic observations may give exact information on the distribution of lectin binding sites.
en-copyright= kn-copyright= en-aut-name=MoriMasaharu en-aut-sei=Mori en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamotoShu en-aut-sei=Nakamoto en-aut-mei=Shu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KirizukaKeizi en-aut-sei=Kirizuka en-aut-mei=Keizi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SadahiraYoshito en-aut-sei=Sadahira en-aut-mei=Yoshito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AwaiMichiyasu en-aut-sei=Awai en-aut-mei=Michiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SenoSatimaru en-aut-sei=Seno en-aut-mei=Satimaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UtsumiKozo en-aut-sei=Utsumi en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Shigei Medical Research Institute affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Kochi Medical School en-keyword=Ehrlich ascites tumor cell kn-keyword=Ehrlich ascites tumor cell en-keyword=concanvalin A kn-keyword=concanvalin A en-keyword=cytochalasin B kn-keyword=cytochalasin B en-keyword=cap formation kn-keyword=cap formation END start-ver=1.4 cd-journal=joma no-vol=43 cd-vols= no-issue=4 article-no= start-page=249 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate on morphology and anchorage-independent growth of normal and established chick embryo cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the organization of cytoskeleton and growth of normal and established chick embryo cells (CEC) were studied. The cytoskeleton of normal CEC formed stress fibers, while that of the CEC lines established in our laboratory formed no stress fibers. TPA treatment of normal CEC resulted in disorganization of the stress fibers into amorphous structure, while that of the established CEC lines induced no reorganization of the cytoskeleton. TPA had no promotional effect in vitro or in vivo on tumor growth in normal or the established CEC.
en-copyright= kn-copyright= en-aut-name=OguraHajime en-aut-sei=Ogura en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujiwaraTazuko en-aut-sei=Fujiwara en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=12-O-tetradecanoyl-phorbol-13-acetate kn-keyword=12-O-tetradecanoyl-phorbol-13-acetate en-keyword=established chick embryo cell lines kn-keyword=established chick embryo cell lines en-keyword=cytoskeleton kn-keyword=cytoskeleton en-keyword=stress fiber kn-keyword=stress fiber en-keyword=anchorage-independent growth kn-keyword=anchorage-independent growth END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=4 article-no= start-page=193 end-page=200 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fluorescence and Electron Microscopic Studies of the Cytoskeletal Organi?zation of Normal, Established and Transformed Chick Embryo Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=The cytoskeletons of two established chick embryo cell (CEC) lines were examined by fluorescence and electron microscopy and compared with those of control cells and cells transformed by Rous sarcoma virus (RSV). In normal CEC, many stress fibers were observed. On the other hand, stress fibers were disorganized in nontransformed spontaneously established CEC, non-tumorigenic CEC partially transformed with a chemical carcinogen, and tumorigenic RSV-transformed CEC. In the normal CEC, actin filaments formed several bundles along the processes of the cell. Stereo-images of the peripheral region revealed bundles of filaments which were located along the attached side to the substrate. A fine well preserved network of filaments was also observed. On the other hand, in spontaneously established, partially transformed and RSV-transformed CEC, a fine network of filaments, but no actin cables, was found. These results support previous evidence that the cytoskeletal changes themselves are not directly related to the transformation or tumorigenicity of cells.
en-copyright= kn-copyright= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NomuraTakako en-aut-sei=Nomura en-aut-mei=Takako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujiwaraTazuko en-aut-sei=Fujiwara en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OguraHajime en-aut-sei=Ogura en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=chick embryo cells kn-keyword=chick embryo cells en-keyword=cytoskeleton kn-keyword=cytoskeleton en-keyword=actin kn-keyword=actin en-keyword=Triton X-100 kn-keyword=Triton X-100 en-keyword= Rous sarcoma virus. kn-keyword= Rous sarcoma virus. END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=4 article-no= start-page=201 end-page=206 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Exocytotic features of rat specific atrial granules. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the mode of secretion of specific atrial granules, rat atrial muscle cells were examined by transmission electron microscopy. Atrial granule formation and exocytotic features of granules were clearly seen. Abrupt breaks in the unit membrane structure of mature granules were observed in thin sections, but these breaks were not detected in freeze-fracture replicas. These findings support the concept that the granule contents are released to the extracellular space by exocytosis.
en-copyright= kn-copyright= en-aut-name=NomuraTakako en-aut-sei=Nomura en-aut-mei=Takako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KurokawaHideo en-aut-sei=Kurokawa en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KandaShigeto en-aut-sei=Kanda en-aut-mei=Shigeto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MizukawaKiminao en-aut-sei=Mizukawa en-aut-mei=Kiminao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OtsukaNagayasu en-aut-sei=Otsuka en-aut-mei=Nagayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=exocytosis kn-keyword=exocytosis en-keyword=atrial natriuretic polypeptide kn-keyword=atrial natriuretic polypeptide en-keyword=rat atrium kn-keyword=rat atrium en-keyword=electron microscopy kn-keyword=electron microscopy END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=6 article-no= start-page=311 end-page=316 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Light and electron microscopic observation of specific atrial granules using water-miscible resin as an embedding medium. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A mixture of glycol methacrylate (GMA) and Quetol 523 was examined as an embedding medium for atrial tissue to be selectively stained for specific atrial granules. Semi-thin sections of rat atrial tissue embedded in this resin were stained with lead hematoxylin and observed under a light microscope. Atrial granules were found to be specifically stained blue black with lead hematoxylin. The same semithin sections stained with OsO4 vapor were examined electron microscopically and the atrial granules could be distinguised clearly from other cytoplasmic components. The GMA-Quetol 523 mixture is a useful embedding medium for studying the distribution of specific atrial granules by light and electron microscopy.
en-copyright= kn-copyright= en-aut-name=NomuraTakako en-aut-sei=Nomura en-aut-mei=Takako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OtsukaNagayasu en-aut-sei=Otsuka en-aut-mei=Nagayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaYukiko en-aut-sei=Tanaka en-aut-mei=Yukiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=specific atrial granules kn-keyword=specific atrial granules en-keyword=glycol methacrylate-Quetol kn-keyword=glycol methacrylate-Quetol en-keyword=lead-hematoxylin kn-keyword=lead-hematoxylin en-keyword=electron microscopy kn-keyword=electron microscopy en-keyword=rat kn-keyword=rat END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=3 article-no= start-page=197 end-page=204 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A scanning electron microscopic study of the two-step effect of cytochalasin B on Ehrlich ascites tumor cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effect of cytochalasin B (CB) on the surface structure of Ehrlich ascites tumor cells was investigated using the scanning electron microscope. The effect occurs in two steps: formation of zeiotic knobs on the cell surface and subsequent grouping of the knobs at one pole of the cell. The early step of zeiotic knob formation occurs at low concentrations of CB (0.5-1 microgram/ml) at 37 degrees C and at high concentrations of the drug (5-10 microgram/ml) at low temperature but within 1 min at 37 degrees C. This step is only partially inhibited by 5 x 10(-3) M sodium azide. The subsequent grouping of zeiotic knobs lasts for more than 2 min at 37 degrees C and occurs only in the case of high concentrations of CB. It is inhibited by sodium azide and is often associated with grouping of the microvilli, which are then lost from all of the cell surface except the area of knob-grouping.
en-copyright= kn-copyright= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ImanakaMasaaki en-aut-sei=Imanaka en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OtsukaNagayasu en-aut-sei=Otsuka en-aut-mei=Nagayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamotoShu en-aut-sei=Nakamoto en-aut-mei=Shu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriMasaharu en-aut-sei=Mori en-aut-mei=Masaharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=cytochalasin B kn-keyword=cytochalasin B en-keyword=Ehrlich ascites tumor cells kn-keyword=Ehrlich ascites tumor cells en-keyword=zeiosis kn-keyword=zeiosis en-keyword= scanning electron microscopy. kn-keyword= scanning electron microscopy. END start-ver=1.4 cd-journal=joma no-vol=41 cd-vols= no-issue=4 article-no= start-page=145 end-page=154 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=198708 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Concanavalin A-induced cap formation in rat ascites hepatoma cells (AH 7974) and the interaction of cytoplasmic proteins with plasma membranes. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Concanavalin A (Con A) induced cap formation in rat ascites hepatoma cells (AH7974). In these Con A-treated cells, the association of cytoplasmic proteins with cell membranes was suggested by observing their Triton shells. The transition from G-actin to F-actin occurred in these cells. The association of membrane lipid with cytoplasmic proteins extracted from AH cells was studied by the isolation of protein-bound liposomes and phase transition release. The analysis of isolated liposomes revealed that many cytoplasmic proteins which specifically associated with liposomes were cytoskeletal elements including F-actins. The association of proteins with liposomes was affected by the lipid composition of the liposomal membrane and by the Ca2+ concentration of the incubation medium. The strong interaction of liposomal membrane with cytoplasmic proteins or isolated cytoskeletal proteins was demonstrated also by phase transition release using carboxy fluorescein-containing liposomes. These experiments showed that there was a strong affinity between lipid membrane and cytoskeletal elements including F-actins and that the amount of F-actin increased due to Con A treatment. The association of the submembranous microfilaments with the cell membrane may contribute to capping of the cells caused by Con A.
en-copyright= kn-copyright= en-aut-name=MoromizatoYasunori en-aut-sei=Moromizato en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=concanavalin A kn-keyword=concanavalin A en-keyword=actins kn-keyword=actins en-keyword=liposomes kn-keyword=liposomes en-keyword=phase trasition release kn-keyword=phase trasition release END start-ver=1.4 cd-journal=joma no-vol=40 cd-vols= no-issue=6 article-no= start-page=301 end-page=311 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=198612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fluorescence and electron microscopic study of intracellular F-actin in concanavalin A-treated and cytochalasin B-treated Ehrlich ascites tumor cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract=To investigate the involvement of actin filaments in concanavalin A (Con A)-induced cap formation and cytochalasin B (CB)-induced zeiotic knob migration, the distribution of F-actin was studied in Con A-treated and CB-treated Ehrlich ascites tumor cells (EATC) by fluorescence microscopy using heavy meromyosin conjugated with a fluorescent dye, N-(7-dimethylamino-4-methylcoumarinyl) maleimide, (DACM-HMM). In non-treated cells, the diffuse fluorescence of DACM-HMM was observed in the cytoplasm, particularly intensely under the plasma membrane and around the nucleus. In Con A- and CB-treated cells, the fluorescence was seen at Con A-induced-capped and CB-induced-knob-accumulated regions. This fluorescence was more intense in CB-treated cells. To study the actin filaments in these fluorescent regions more clearly, the soluble components of the cells were eliminated by treatment with Triton X-100 or saponin solution containing a low concentration of glutaraldehyde, and the detergent-treated and saponin-treated cells were observed under a transmission electron microscope. Concentrated actin filaments were observed directly beneath the Con A-induced capping area and CB-induced zeiotic knob-accumulation area. The area of concentrated actin filaments appeared to correspond to the electron dense area observed in the identical region in the cells fixed without detergent treatment. More actin filaments were observed in CB-treated cells than in Con A-treated ones.
en-copyright= kn-copyright= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeharaYoshiki en-aut-sei=Takehara en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiiYoshitaka en-aut-sei=Fujii en-aut-mei=Yoshitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkimasuEiji en-aut-sei=Okimasu en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoromizatoYasunori en-aut-sei=Moromizato en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=DACM-HMM kn-keyword=DACM-HMM en-keyword=Ehrlich ascites tumor cells kn-keyword=Ehrlich ascites tumor cells en-keyword=concanavalin A kn-keyword=concanavalin A en-keyword=cytochalasin B kn-keyword=cytochalasin B en-keyword=actim kn-keyword=actim en-keyword=capping kn-keyword=capping END start-ver=1.4 cd-journal=joma no-vol=37 cd-vols= no-issue=5 article-no= start-page=385 end-page=391 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=198310 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Inhibition of Phospholipase A2 and Platelet Aggregation by Grycyrrhizin, an Anti-inframmation Drug en-subtitle= kn-subtitle= en-abstract= kn-abstract=We studied the effect of glycyrrhizin, a compound known as an anti-inflammatory and antiallergic drug, on the membrane permeability change induced by phospholipase A2 (PLA2) and on platelet aggregation. Glycyrrhizin was found to inhibit the PLA2-induced carboxyfluorescein (CF) release from D,L-dipalmitoyl phosphatidylcholine (DPPC) liposomes. Part of this inhibitory effect of glycyrrhizin on PLA2 is accounted for by the physical state of the substrate, the DPPC liposome membrane. Glycyrrhizin also inhibited collagen-induced platelet aggregation in a concentration dependent manner, which may in part account for its inhibitory effect on PLA2.
en-copyright= kn-copyright= en-aut-name=OkimasuEiji en-aut-sei=Okimasu en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MoromizatoYasunori en-aut-sei=Moromizato en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSadahiro en-aut-sei=Watanabe en-aut-mei=Sadahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShiraishiNoriyuki en-aut-sei=Shiraishi en-aut-mei=Noriyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MorimotoYasuko M en-aut-sei=Morimoto en-aut-mei=Yasuko M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MiyaharaMasanobu en-aut-sei=Miyahara en-aut-mei=Masanobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UtsumiKozo en-aut-sei=Utsumi en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=phosholipase A2 kn-keyword=phosholipase A2 en-keyword=glycyrrhizin kn-keyword=glycyrrhizin en-keyword=liposome kn-keyword=liposome en-keyword=platelet aggregation kn-keyword=platelet aggregation END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=5 article-no= start-page=283 end-page=298 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Histological Observation of the Development of Follicles and Follicular Atresia in Immature Rat Ovaries en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the development of follicular growth and atresia in the immature ovary, rats. ovaries and blood were removed at fixed points during the period from 0 to 35 days after birth (Day 0 to Day 35). The ovaries were immunohistochemically examined, and blood concentrations of serum follicle-stimulating hormone (FSH) and estrogen (E) were measured. We investigated how time-course changes in follicular cell proliferation, estrogen receptor β (ERβ), apoptosis, and FSH and E concentrations are connected with follicular growth and atresia. Apoptosis was found in the ova from Day 0 to Day 3. On Day 15, apoptosis occurred in some granulosa cell nuclei in some follicles, but BrdU uptake and the presence of cyclin D2 and ER β could be observed in other granulosa cells. From Day 17, apoptosis increased in the follicular granulosa cells, and BrdU uptake and the presence of cyclin D2 and ERβ were decreased. Follicular atresia continued, reaching a peak on Day 30. Serum FSH and E concentrations increased until Day 15, then markedly decreased after Day 17. The mechanism of apoptosis in the ova from Day 0 to 3 has not been clarified. However, the onset of follicular atresia was caused by apoptotic degeneration from Days 15 to 17. These results showed that the oocytes were selected by apoptosis at 2 points in the time-course of the maturation of the ovary. en-copyright= kn-copyright= en-aut-name=TakagiKoji en-aut-sei=Takagi en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamadaTeruo en-aut-sei=Yamada en-aut-mei=Teruo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MikiYukari en-aut-sei=Miki en-aut-mei=Yukari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UmegakiTeruo en-aut-sei=Umegaki en-aut-mei=Teruo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishimuraMakoto en-aut-sei=Nishimura en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=histology kn-keyword=histology en-keyword=apoptosis kn-keyword=apoptosis en-keyword=proliferation kn-keyword=proliferation en-keyword=estrogen kn-keyword=estrogen en-keyword=follicle-stimulating hormone kn-keyword=follicle-stimulating hormone END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=19750331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=分離したラツト肥満細胞からのα-キモトリプシンによるヒスタミン遊離の機構 kn-title=Mechanism of histamine release by alpha-cymotrypsin from isolated rat mast cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=佐々木順造 kn-aut-sei=佐々木 kn-aut-mei=順造 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=95 end-page=98 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19950131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Oyygen uptake of adriamycin resistant cells of Ehrlich ascites tumor kn-title=アドリアマイシン耐性細胞の酸素呼吸 en-subtitle= kn-subtitle= en-abstract=Adriamycin-resistant cells of Ehrlich ascites tumor cells were established in our laboratory. Using electron microscope, the area of mitochondria (MT) per cytoplasm of ADR-resistant cells were measured with planimeter. The values of wild-type cells, 1μg/ml ADR-resistant cells and 10μg/ml ADR-resistant cells were 39.3, 51.8 and 57.7 μ(2) per 1,000 μ(2) of cytoplasm, respectively. Oxygen consumption of 1 μg/ml ADR-resistant cells and 10 μg/ml ADR-resistant cells were 1.45-fold and 1.49-fold compared to that of wild-type cells, respectively. These results indicate that ADR-resistant cells require more energy to work efflux pump than wild-type cells. kn-abstract=エールリッヒ腹水癌細胞を用いアドリアマイシンに対する耐性細胞（ADR耐性細胞）を樹立した。電子顕微鏡を用い撮影写真から細胞質当たりのミトコンドリア（MT）の割合を面積比で求めた。親株に比較して1μg/ml ADR耐性細胞では1.32倍、10μg/ml ADR耐性細胞では1.47倍であった。これらの細胞の呼吸を測定した。耐性細胞の内発呼吸は親株に比較して増加していた。1μg/ml ADR耐性細胞では1.45倍、10μg/ml ADR耐性細胞では1.49倍であり、MTの増加量とほぼ同じ割合であった。これらのことから、細胞が耐性になるとエネルギー消費が高まるために細胞内MTが増加し、その結果呼吸（酸素消費）が増加することが推察された。 en-copyright= kn-copyright= en-aut-name=KawasakiShoji en-aut-sei=Kawasaki en-aut-mei=Shoji kn-aut-name=川ｱ祥二 kn-aut-sei=川ｱ kn-aut-mei=祥二 aut-affil-num=1 ORCID= en-aut-name=NomuraTakako en-aut-sei=Nomura en-aut-mei=Takako kn-aut-name=野村貴子 kn-aut-sei=野村 kn-aut-mei=貴子 aut-affil-num=2 ORCID= en-aut-name=MatsuuraJunko en-aut-sei=Matsuura en-aut-mei=Junko kn-aut-name=松浦順子 kn-aut-sei=松浦 kn-aut-mei=順子 aut-affil-num=3 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name=佐々木順造 kn-aut-sei=佐々木 kn-aut-mei=順造 aut-affil-num=4 ORCID= en-aut-name=GaoXian Shu en-aut-sei=Gao en-aut-mei=Xian Shu kn-aut-name=高献書 kn-aut-sei=高 kn-aut-mei=献書 aut-affil-num=5 ORCID= en-aut-name=AsaumiJun-ichi en-aut-sei=Asaumi en-aut-mei=Jun-ichi kn-aut-name=浅海淳一 kn-aut-sei=浅海 kn-aut-mei=淳一 aut-affil-num=6 ORCID= en-aut-name=Nishikawakouji en-aut-sei=Nishikawa en-aut-mei=kouji kn-aut-name=西川光治 kn-aut-sei=西川 kn-aut-mei=光治 aut-affil-num=7 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name=平木祥夫 kn-aut-sei=平木 kn-aut-mei=祥夫 aut-affil-num=8 ORCID= en-aut-name=UtsumiKozo en-aut-sei=Utsumi en-aut-mei=Kozo kn-aut-name=内海耕慥 kn-aut-sei=内海 kn-aut-mei=耕慥 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部診療放射線技術学科 affil-num=2 en-affil= kn-affil=岡山大学医学部第1解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第1解剖学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第1解剖学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=9 en-affil= kn-affil=倉敷成人病センター en-keyword=アドリアマイシン (adriamycin) kn-keyword=アドリアマイシン (adriamycin) en-keyword=多剤耐性 (multidrugs resistant) kn-keyword=多剤耐性 (multidrugs resistant) en-keyword=酸素消費 (oxygen uptake) kn-keyword=酸素消費 (oxygen uptake) en-keyword=呼吸 (respiration) kn-keyword=呼吸 (respiration) en-keyword=ミトコンドリア (mitochondria) kn-keyword=ミトコンドリア (mitochondria) END start-ver=1.4 cd-journal=joma no-vol=1206 cd-vols= no-issue= article-no= start-page=1 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080623 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=alpha-lipoic acid suppresses 6-hydroxydoparnine-induced ROS generation and apoptosis through the stimulation of glutathione synthesis but not by the expression of heme oxygenase-1 en-subtitle= kn-subtitle= en-abstract= kn-abstract=We previously reported that the generation of reactive oxygen species (ROS) is the initial event in cell death induced by 6-hydroxydopamine (6-OHDA), an experimental model of Parkinsonism. Since recent studies suggested the important role of antioxidant activity of alpha-lipoic acid (LA) in the suppression of apoptosis of various types, we studied the effect on 6-OHDA-induced apoptosis of PC12 cells. Biochemical analysis revealed that LA suppressed the 6-OHDA-induced ROS generation, increase of caspase-like activity and chromatin condensation. The suppression of 6-OHDA-induced apoptosis by LA required pre-incubation of PC12 cells with LA for 12-24 h. LA increased the intracellular levels of heme oxygenase-1 (HO-1) and glutathione (GSH) and stimulated the expression of GSH synthesis-related genes such as cystine/glutamate antiporter and gamma-glutamylcysteine synthetase (gamma-GCS). However, Sn-mesoporphyrin IX, an inhibitor of HO-1, did not attenuate the LA-induced suppression of apoptosis. In contrast, buthionine sulfoximine, an inhibitor of gamma-GCS, attenuated the LA-induced suppression of ROS generation and chromatin condensation. in addition, a transcription factor Nrf2, which regulates the expression of antioxidant enzymes such as gamma-GCS, translocated to the nucleus by LA. These results suggested that LA suppressed the 6-OHDA induced-apoptosis by the increase in cellular glutathione through stimulation of the GSH synthesis system but not by the expression of HO-1.
en-copyright= kn-copyright= en-aut-name=FujitaHirofumi en-aut-sei=Fujita en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShiosakaMasahiko en-aut-sei=Shiosaka en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OginoTetsuya en-aut-sei=Ogino en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkimuraYuya en-aut-sei=Okimura en-aut-mei=Yuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=UtsumiToshihiko en-aut-sei=Utsumi en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SatoEisuke F. en-aut-sei=Sato en-aut-mei=Eisuke F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AkagiReiko en-aut-sei=Akagi en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=InoueMasayasu en-aut-sei=Inoue en-aut-mei=Masayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=UtsumiKozo en-aut-sei=Utsumi en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University affil-num=6 en-affil= kn-affil=Department of Biochemistry and Molecular Pathology Osaka City University Medical School affil-num=7 en-affil= kn-affil=Faculty of Health and Welfare Science, Okayama Prefectural University affil-num=8 en-affil= kn-affil=Department of Biochemistry and Molecular Pathology Osaka City University Medical School affil-num=9 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=apoptosis kn-keyword=apoptosis en-keyword=glutathione kn-keyword=glutathione en-keyword=gamma-glutamylcysteine synthetase; heme oxygenase-1 kn-keyword=gamma-glutamylcysteine synthetase; heme oxygenase-1 en-keyword=6-hydroxydopamine kn-keyword=6-hydroxydopamine en-keyword=alpha-lipoic acid kn-keyword=alpha-lipoic acid en-keyword=Nrf2 kn-keyword=Nrf2 END