Title Alternative Increase in Cytochorome c and a 11.9 kDa protein in Submerged Rice Seedlings after Exposure to Air
FullText URL 002_002_149_157.pdf
Author Shibasaka, Mineo| Ushimaru, Takashi| Ookubo, Katsuyuki| Tsuchida, Shin-ichi| Tsuji, Hideo|
Abstract To examine the changes in cytocrome c content in submerged rice seedlings after exposure to air, antiserum was prepared against purified cytocrome c from rice bran. Western blottong analysis revealed that cytochrome c was detected 6 h after exposure to air, but not detected in submerged rice seedling. On a fresh weight basis, the same level of cytochrome c as that of the aerobic control was found in the 24-h-air adapted seedlings. judging from the high A408/A280 ratio (4.66),the cytochrome c preparation used as antigen was considered to be well purified. However, the antiserum reacted other several polypeptides. One of them reacted more strongly against the antisermu than cytochrome c and its molecular weight was estimated as 11.9 kDa. The polypeptide increased during air adaptation and the levels found in both submerged seedlings and aerobic control were lower than that in 24-h-air-adapted seedlings.
Keywords Air-adaptation Anti-cytochrome c serum Cytochrome c Cytocrome c purification Oryza sativa
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1994
Volume volume2
Issue issue2
Start Page 149
End Page 157
ISSN 0916-930X
language 日本語
File Version publisher
Title Alternative Changes in Mitochondrial Spectra of Submerged Rice Seedlings after Exposure to Air Gaussian Deconvolution Analysis
FullText URL 002_002_135_147.pdf
Author Shibasaka, Mineo| Tsuji, Hideo|
Abstract Quantitative changes in mitochondrial cytochromes of submerged rice seedlings after exposure to air were investigated using gaussian deconvolution analysis on absorption spectrum at liquid nitrogen temperature. Differnece spectra were obtained from subtracting the absorption spectra of oxidized mitochondria from reduced mitochondria by succinate with antimycin-A. they showed three kinds of b-type cytochrome and a spectral component similar to cytochrome c1. Subtraction of the cytochrome c1-like component from the spectrum by curve analysis showed that three cytochrome bs had similar absorption maximum and that on a mitochondrial protein basis they were constant during air-adaptation. Cytochrome c per mitochontorial protein in submerged seedlings was about half of that in aerobic seedlings and increased by 1.5-fold during air-adaptation. The change in cytochrome c content was in paralleled with that of cytochrome aa3. These findings revealed that there were two groups of cytochromes in the response to air-adaptation,i.e.,three cytochrome bs were constant and cytochoromes c and aa3 increased in parallel.
Keywords Absorption spectrum Air-adaptation Cytocrome Gaussian deconvolution analysis Oryza sativa
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1994
Volume volume2
Issue issue2
Start Page 135
End Page 147
ISSN 0916-930X
language 日本語
File Version publisher
Title Alternative Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification
FullText URL 003_002_137_143.pdf
Author Akiyama, Yoshiko| Shibasaka, Mineo| Kawasaki, Toshio|
Abstract Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged.
Keywords Adenylate extraction ADP AMP ATP Barley roots
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue2
Start Page 137
End Page 143
ISSN 0916-930X
language 日本語
File Version publisher
Title Alternative Effects of Mannosen on Hydroponically Grown Barley
FullText URL 003_002_129_135.pdf
Author Shibasaka, Mineo| Miyata, Masahiko| Akiyama, Yoshiko| Kawasaki, Toshio|
Abstract To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants.
Keywords Barley Hydroponics Mannose Potassium Phosphate
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue2
Start Page 129
End Page 135
ISSN 0916-930X
language 日本語
File Version publisher
Author Shibasaka, Mineo| Sasano, Sizuka| Utsugi, Sigeko| Katsuhara, Maki|
Published Date 2012-12
Publication Title Plant Signaling & Behavior
Volume volume7
Issue issue12
Content Type Journal Article