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ID 11282
Eprint ID
11282
FullText URL
Title Alternative
Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification
Author
Akiyama, Yoshiko
Kawasaki, Toshio
Abstract
Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged.
Abstract Alternative
高等植物根のアデニル酸をルシフェリン-ルシフェラーゼ法による定量に適する抽出法を捜すために、数種の抽出法を比べた。過塩素酸で抽出し、オクチルアミンで中和し、フロンの一種で洗う方法が最も抽出効果が高く、また比較的操作が簡便であった。
Keywords
Adenylate extraction
ADP
AMP
ATP
Barley roots
Published Date
1995
Publication Title
岡山大学資源生物科学研究所報告
Publication Title Alternative
Bulletin of the Research Institute for Bioresources, Okayama University
Volume
volume3
Issue
issue2
Publisher
岡山大学資源生物科学研究所
Publisher Alternative
Research Institute for Bioresources, Okayama University
Start Page
137
End Page
143
ISSN
0916-930X
NCID
AN10381600
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False