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Ohtsuki, Takashi Department of Medical Technology, Graduate School of Health Sciences, Okayama University ORCID Kaken ID publons researchmap
Hatipoglu, Omer F. Department of Medical Technology, Graduate School of Health Sciences, Okayama University
Asano, Keiichi Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Inagaki, Junko Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID publons researchmap
Nishida, Keiichiro Department of Orthopaediac Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID publons researchmap
Hirohata, Satoshi Department of Medical Technology, Graduate School of Health Sciences, Okayama University ORCID Kaken ID publons researchmap
Abstract
In patients with osteoarthritis (OA), there is a decrease in both the concentration and molecular size of hyaluronan (HA) in the synovial fluid and cartilage. Cell migration-inducing hyaluronidase 1 (CEMIP), also known as hyaluronan (HA)-binding protein involved in HA depolymerization (HYBID), was recently reported as an HA depolymerization-related molecule expressed in the cartilage of patients with OA. However, the underlying mechanism of CEMIP regulation is not well understood. We found that CEMIP expression was transiently increased by interleukine-1 beta (IL-1 beta) stimulation in chondrocytic cells. We also observed that ERK activation and NF-kappa B nuclear translocation were involved in the induction of CEMIP by IL-1 beta. In addition, both administration of HA and mechanical strain attenuated the CEMIP induction in IL-1 beta-stimulated chondrocytes. In conclusion, we clarified the regulatory mechanism of CEMIP in chondrocytes by inflammatory cytokines and suggested the potential involvement in osteoarthritis development.
Keywords
cell migration-inducing hyaluronidase 1 (CEMIP)
chondrocyte
hyaluronan
mechanical strain
nuclear factor kappa B (NF-kappa B)
osteoarthritis
Published Date
2020-04-29
Publication Title
International Journal of Molecular Sciences
Volume
volume21
Issue
issue9
Publisher
MDPI
Start Page
3140
ISSN
1422-0067
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 by the authors.
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.3390/ijms21093140
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
17K19727
17H04313
19K09627
19K11791