start-ver=1.4 cd-journal=joma no-vol=1206 cd-vols= no-issue= article-no= start-page=1 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080623 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=alpha-lipoic acid suppresses 6-hydroxydoparnine-induced ROS generation and apoptosis through the stimulation of glutathione synthesis but not by the expression of heme oxygenase-1 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

We previously reported that the generation of reactive oxygen species (ROS) is the initial event in cell death induced by 6-hydroxydopamine (6-OHDA), an experimental model of Parkinsonism. Since recent studies suggested the important role of antioxidant activity of alpha-lipoic acid (LA) in the suppression of apoptosis of various types, we studied the effect on 6-OHDA-induced apoptosis of PC12 cells. Biochemical analysis revealed that LA suppressed the 6-OHDA-induced ROS generation, increase of caspase-like activity and chromatin condensation. The suppression of 6-OHDA-induced apoptosis by LA required pre-incubation of PC12 cells with LA for 12-24 h. LA increased the intracellular levels of heme oxygenase-1 (HO-1) and glutathione (GSH) and stimulated the expression of GSH synthesis-related genes such as cystine/glutamate antiporter and gamma-glutamylcysteine synthetase (gamma-GCS). However, Sn-mesoporphyrin IX, an inhibitor of HO-1, did not attenuate the LA-induced suppression of apoptosis. In contrast, buthionine sulfoximine, an inhibitor of gamma-GCS, attenuated the LA-induced suppression of ROS generation and chromatin condensation. in addition, a transcription factor Nrf2, which regulates the expression of antioxidant enzymes such as gamma-GCS, translocated to the nucleus by LA. These results suggested that LA suppressed the 6-OHDA induced-apoptosis by the increase in cellular glutathione through stimulation of the GSH synthesis system but not by the expression of HO-1.

en-copyright= kn-copyright= en-aut-name=FujitaHirofumi en-aut-sei=Fujita en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShiosakaMasahiko en-aut-sei=Shiosaka en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OginoTetsuya en-aut-sei=Ogino en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkimuraYuya en-aut-sei=Okimura en-aut-mei=Yuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=UtsumiToshihiko en-aut-sei=Utsumi en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SatoEisuke F. en-aut-sei=Sato en-aut-mei=Eisuke F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AkagiReiko en-aut-sei=Akagi en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=InoueMasayasu en-aut-sei=Inoue en-aut-mei=Masayasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=UtsumiKozo en-aut-sei=Utsumi en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SasakiJunzo en-aut-sei=Sasaki en-aut-mei=Junzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Pathological Research, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University affil-num=6 en-affil= kn-affil=Department of Biochemistry and Molecular Pathology Osaka City University Medical School affil-num=7 en-affil= kn-affil=Faculty of Health and Welfare Science, Okayama Prefectural University affil-num=8 en-affil= kn-affil=Department of Biochemistry and Molecular Pathology Osaka City University Medical School affil-num=9 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=apoptosis kn-keyword=apoptosis en-keyword=glutathione kn-keyword=glutathione en-keyword=gamma-glutamylcysteine synthetase; heme oxygenase-1 kn-keyword=gamma-glutamylcysteine synthetase; heme oxygenase-1 en-keyword=6-hydroxydopamine kn-keyword=6-hydroxydopamine en-keyword=alpha-lipoic acid kn-keyword=alpha-lipoic acid en-keyword=Nrf2 kn-keyword=Nrf2 END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=4 article-no= start-page=237 end-page=242 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200608 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Angiomyofibroblastoma of the vulva: a large pedunculated mass formation. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Angiomyofibroblastoma is a rare, usually small benign mesenchymal tumor that occurs in vulvar lesions of premenopausal women. A case of angiomyofibroblastoma that arose as a unique pedunculated and particularly large mass in the left vulva of a 48-year-old woman is presented herein. The patient had been aware of a gradually enlarged mass of 7 years duration without any other gynecological symptoms or signs. The maximum dimension of the tumor measured 11 cm. The resected tumor was well circumscribed with a bulging and glistening cut surface. Histological examination revealed an admixture of irregularly distributed hypercellular and hypocellular areas with spindled, plump spindled, or plasmacytoid stromal cells and abundant venular or capillary-sized vessels. Stromal cells characteristically cluster around delicate vessels within an edematous to collagenous matrix. In the present case, intralesional adipose tissue was present throughout the tumor. There was no significant nuclear atypia, and mitotic figures were very sparse. There was little stromal mucin throughout the tumor. Immunohistochemically, the stromal cells were characterized by strong reactivity for vimentin and CD34, with focal reactivity for desmin and alpha smooth muscle actin. Both estrogen and progesterone receptors were diffusely expressed in the stromal cells. These histological findings are consistent with angiomyofibroblastoma and support the hypothesis that angiomyofibroblastoma originates from perivascular stem cells with a capacity for myofibroblastic and fatty differentiation. en-copyright= kn-copyright= en-aut-name=OmoriMasako en-aut-sei=Omori en-aut-mei=Masako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ToyodaHiroshi en-aut-sei=Toyoda en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HiraiTakeshi en-aut-sei=Hirai en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OginoTetsuya en-aut-sei=Ogino en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkadaShigeru en-aut-sei=Okada en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama Kyoritsu Hospital affil-num=3 en-affil= kn-affil=Okayama Kyoritsu Hospital affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=angiomyofibroblastoma kn-keyword=angiomyofibroblastoma en-keyword=vulva kn-keyword=vulva en-keyword=adipose tissue kn-keyword=adipose tissue en-keyword=pedunculated mass kn-keyword=pedunculated mass END start-ver=1.4 cd-journal=joma no-vol=117 cd-vols= no-issue=3 article-no= start-page=187 end-page=191 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20060104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=活性酸素によるRB タンパクの酸化を介した細胞周期停止 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=寳迫睦美 kn-aut-sei=寳迫 kn-aut-mei=睦美 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=荻野哲也 kn-aut-sei=荻野 kn-aut-mei=哲也 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡田茂 kn-aut-sei=岡田 kn-aut-mei=茂 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 病態探究医学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 病態探究医学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 病態探究医学 en-keyword=細胞周期 kn-keyword=細胞周期 en-keyword=RB タンパク kn-keyword=RB タンパク en-keyword=活性酸素 kn-keyword=活性酸素 en-keyword=モノクロラミン kn-keyword=モノクロラミン en-keyword=酸化ストレス kn-keyword=酸化ストレス END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=19880328 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=パラコート中毒マウスでのクエン酸鉄錯体による脂質過酸化及び組織障害作用 kn-title=Lipid peroxidation and tissue injury by ferric citrate in paraquat intoxicated mice en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=荻野哲也 kn-aut-sei=荻野 kn-aut-mei=哲也 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END