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Yamashita, Toru Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap
Kushida, Yoshihiro Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine
Wakao, Shohei Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine
Tadokoro, Koh Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nomura, Emi Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Omote, Yoshio Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takemoto, Mami Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID
Hishikawa, Nozomi Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID
Ohta, Yasuyuki Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID researchmap
Dezawa, Mari Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine
Abe, Koji Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID publons researchmap
Abstract
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive motor neuron loss. Muse cells are endogenous reparative pluripotent-like stem cells distributed in various tissues. They can selectively home to damaged sites after intravenous injection by sensing sphingosine-1-phosphate produced by damaged cells, then exert pleiotropic effects, including tissue protection and spontaneous differentiation into tissue-constituent cells. In G93A-transgenic ALS mice, intravenous injection of 5.0x10(4) cells revealed successful homing of human-Muse cells to the lumbar spinal cords, mainly at the pia-mater and underneath white matter, and exhibited glia-like morphology and GFAP expression. In contrast, such homing or differentiation were not recognized in human mesenchymal stem cells but were instead distributed mainly in the lung. Relative to the vehicle groups, the Muse group significantly improved scores in the rotarod, hanging-wire and muscle strength of lower limbs, recovered the number of motor neurons, and alleviated denervation and myofiber atrophy in lower limb muscles. These results suggest that Muse cells homed in a lesion site-dependent manner and protected the spinal cord against motor neuron death. Muse cells might also be a promising cell source for the treatment of ALS patients.
Keywords
Mesenchymal stem cells
Neurological disorders
Published Date
2020-10-13
Publication Title
Scientific Reports
Volume
volume10
Issue
issue1
Publisher
Nature Research
Start Page
17102
ISSN
2045-2322
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2020
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publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1038/s41598-020-74216-4
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Agency for Medical Research and Development
助成番号
7211700121
7211800049
7211800130