start-ver=1.4
cd-journal=joma
no-vol=375
cd-vols=
no-issue=3
article-no=
start-page=743
end-page=754
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20181030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Runx3 regulates folliculogenesis and steroidogenesis in granulosa cells of immature mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= We previously demonstrated that female Runx3 knockout (Runx3-/-) mice were anovulatory and their uteri were atrophic and that Runx3 mRNA was expressed in granulosa cells. To clarify how Runx3 regulates folliculogenesis and ovulation, we examine the effects of Runx3 knockout on the gene expression of growth factors associated with folliculogenesis and enzymes associated with steroidogenesis. In Runx3-/- mouse ovaries, the numbers of primary and antral follicles were lower than those in wild-type (wt) mice at 3 weeks of age, indicating that the loss of Runx3 affects folliculogenesis. The expression of genes encoding activin and inhibin subunits (Inha, Inhba and Inhbb) was also decreased in ovaries from the Runx3-/- mice compared with that in wt mice. Moreover, the expression of the genes Cyp11a1 and Cyp19a1 encoding steroidogenic enzymes was also decreased. In cultured granulosa cells from 3-week-old mouse ovaries, Cyp19a1 mRNA levels were lower in Runx3-/- mice than those in wt mice. Follicle-stimulating hormone (FSH) treatment increased Cyp19a1 mRNA levels in both wt and Runx3-/- granulosa cells in culture but the mRNA level in Runx3-/- granulosa cells was lower than that in wt ones, indicating that granulosa cells could not fully function in the absence of Runx3. At 3 weeks of age, gonadotropin α subunit, FSHβ subunit and luteinizing hormone (LH) β subunit mRNA levels were decreased in Runx3-/- mice. These findings suggest that Runx3 plays a key role in female reproduction by regulating folliculogenesis and steroidogenesis in granulosa cells.
en-copyright=
kn-copyright=

en-aut-name=OjimaFumiya
en-aut-sei=Ojima
en-aut-mei=Fumiya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=SaitoYuka
en-aut-sei=Saito
en-aut-mei=Yuka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=TsuchiyaYukiko
en-aut-sei=Tsuchiya
en-aut-mei=Yukiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=OgoshiMaho
en-aut-sei=Ogoshi
en-aut-mei=Maho
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=FukamachiHiroshi
en-aut-sei=Fukamachi
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=InagakiKenichi
en-aut-sei=Inagaki
en-aut-mei=Kenichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OtsukaFumio
en-aut-sei=Otsuka
en-aut-mei=Fumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=TakeuchiSakae
en-aut-sei=Takeuchi
en-aut-mei=Sakae
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=TakahashiSumio
en-aut-sei=Takahashi
en-aut-mei=Sumio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=2
en-affil=Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=3
en-affil=Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=5
en-affil=The Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
kn-affil=

affil-num=6
en-affil=The Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=7
en-affil=The Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
kn-affil=

affil-num=8
en-affil=Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
kn-affil=

affil-num=9
en-affil=Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Estrogen
kn-keyword=Estrogen
en-keyword=Follicle
kn-keyword=Follicle
en-keyword=Mouse
kn-keyword=Mouse
en-keyword=Ovary
kn-keyword=Ovary
en-keyword=Runx3
kn-keyword=Runx3
END

start-ver=1.4
cd-journal=joma
no-vol=33
cd-vols=
no-issue=
article-no=
start-page=31
end-page=34
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2017
dt-pub=201704
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis
kn-title=下垂体隆起部の発生期に特異的に発現する遺伝子Cytokine-like 1,Gap junction protein alpha 5の同定
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars
intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well
studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during
the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development.
en-copyright=
kn-copyright=

en-aut-name=AizawaSayaka 
en-aut-sei=Aizawa
en-aut-mei=Sayaka 
kn-aut-name=相澤清香
kn-aut-sei=相澤
kn-aut-mei=清香
aut-affil-num=1
ORCID=

en-aut-name=HigakiYuriko
en-aut-sei=Higaki
en-aut-mei=Yuriko
kn-aut-name=檜垣佑理子
kn-aut-sei=檜垣
kn-aut-mei=佑理子
aut-affil-num=2
ORCID=

en-aut-name=OgoshiMaho
en-aut-sei=Ogoshi
en-aut-mei=Maho
kn-aut-name=御輿真穂
kn-aut-sei=御輿
kn-aut-mei=真穂
aut-affil-num=3
ORCID=

en-aut-name=TakeuchiSakae
en-aut-sei=Takeuchi
en-aut-mei=Sakae
kn-aut-name=竹内栄
kn-aut-sei=竹内
kn-aut-mei=栄
aut-affil-num=4
ORCID=

en-aut-name=TakahashiSumio
en-aut-sei=Takahashi
en-aut-mei=Sumio
kn-aut-name=高橋純夫
kn-aut-sei=高橋
kn-aut-mei=純夫
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科

affil-num=2
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科

affil-num=4
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科高次生物科学講座･生体統御学分野

affil-num=5
en-affil=Graduate School of Natural Science and Technology, Okayama University
kn-affil=岡山大学大学院自然科学研究科高次生物科学講座･生体統御学分野
END

start-ver=1.4
cd-journal=joma
no-vol=32
cd-vols=
no-issue=
article-no=
start-page=19
end-page=21
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2016
dt-pub=201604
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Structure and expression of quail PBCF gene
kn-title=ウズラPBCF 遺伝子の構造と発現
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=KugimotoAyako
en-aut-sei=Kugimoto
en-aut-mei=Ayako
kn-aut-name=釘本綾子
kn-aut-sei=釘本
kn-aut-mei=綾子
aut-affil-num=1
ORCID=

en-aut-name=AizawaSayaka
en-aut-sei=Aizawa
en-aut-mei=Sayaka
kn-aut-name=相澤清香
kn-aut-sei=相澤
kn-aut-mei=清香
aut-affil-num=2
ORCID=

en-aut-name=OgoshiMaho
en-aut-sei=Ogoshi
en-aut-mei=Maho
kn-aut-name=御輿真穂
kn-aut-sei=御輿
kn-aut-mei=真穂
aut-affil-num=3
ORCID=

en-aut-name=TakahashiSumio
en-aut-sei=Takahashi
en-aut-mei=Sumio
kn-aut-name=高橋純夫
kn-aut-sei=高橋
kn-aut-mei=純夫
aut-affil-num=4
ORCID=

en-aut-name=TakeuchiSakae
en-aut-sei=Takeuchi
en-aut-mei=Sakae
kn-aut-name=竹内栄
kn-aut-sei=竹内
kn-aut-mei=栄
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=4
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=5
en-affil=
kn-affil=岡山大学大学院自然科学研究科
END

start-ver=1.4
cd-journal=joma
no-vol=31
cd-vols=
no-issue=
article-no=
start-page=37
end-page=39
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2015
dt-pub=201504
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Analysis of Kallikrein expression in the mouse endometrium
kn-title=マウス子宮内膜細胞におけるKallikrein の発現制御の解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=TokumoriMegumi
en-aut-sei=Tokumori
en-aut-mei=Megumi
kn-aut-name=徳森萌美
kn-aut-sei=徳森
kn-aut-mei=萌美
aut-affil-num=1
ORCID=

en-aut-name=OgoshiMaho
en-aut-sei=Ogoshi
en-aut-mei=Maho
kn-aut-name=御輿真穂
kn-aut-sei=御輿
kn-aut-mei=真穂
aut-affil-num=2
ORCID=

en-aut-name=TakeuchiSakae
en-aut-sei=Takeuchi
en-aut-mei=Sakae
kn-aut-name=竹内栄
kn-aut-sei=竹内
kn-aut-mei=栄
aut-affil-num=3
ORCID=

en-aut-name=TakahashiSumio
en-aut-sei=Takahashi
en-aut-mei=Sumio
kn-aut-name=高橋純夫
kn-aut-sei=高橋
kn-aut-mei=純夫
aut-affil-num=4
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院自然科学研究科生体統御学グループ

affil-num=2
en-affil=
kn-affil=岡山大学大学院自然科学研究科生体統御学グループ

affil-num=3
en-affil=
kn-affil=岡山大学大学院自然科学研究科生体統御学グループ

affil-num=4
en-affil=
kn-affil=岡山大学大学院自然科学研究科生体統御学グループ
END

start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=
article-no=
start-page=39
end-page=41
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201404
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Breed difference in the regulation of feather coloration in chickens
kn-title=ニワトリにおける羽色調節の品種差
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=TakahashiToru
en-aut-sei=Takahashi
en-aut-mei=Toru
kn-aut-name=高橋徹
kn-aut-sei=高橋
kn-aut-mei=徹
aut-affil-num=1
ORCID=

en-aut-name=NishioKaori
en-aut-sei=Nishio
en-aut-mei=Kaori
kn-aut-name=西尾香織
kn-aut-sei=西尾
kn-aut-mei=香織
aut-affil-num=2
ORCID=

en-aut-name=OgoshiMaho
en-aut-sei=Ogoshi
en-aut-mei=Maho
kn-aut-name=御輿真穂
kn-aut-sei=御輿
kn-aut-mei=真穂
aut-affil-num=3
ORCID=

en-aut-name=TakahashiSumio
en-aut-sei=Takahashi
en-aut-mei=Sumio
kn-aut-name=高橋純夫
kn-aut-sei=高橋
kn-aut-mei=純夫
aut-affil-num=4
ORCID=

en-aut-name=TakeuchiSakae
en-aut-sei=Takeuchi
en-aut-mei=Sakae
kn-aut-name=竹内栄
kn-aut-sei=竹内
kn-aut-mei=栄
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=2
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=3
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=4
en-affil=
kn-affil=岡山大学大学院自然科学研究科

affil-num=5
en-affil=
kn-affil=岡山大学大学院自然科学研究科
END