Among the 3 mitogen-activated protein kinases -- ERK, p38 MAPK and JNK -- JNK has been suggested to participate in apoptosis, whereas p38 MAPK is thought to be part of the differentiation response. There are many common inducers of JNK and p38 MAPK, but the mechanisms underlying the differential response to apoptosis and differentiation are poorly understood. We found that heatshock activated p38 MAPK at 3min after exposure to a temperature of 44 in stress-hypersensitive PC12m3 mutant cells, while it activated JNK at 20min after the same heat treatment. However, heat shock activated p38 MAPK 5min after heat treatment and JNK 10min after heat treatment in PC12 parental cells. The extent of phosphorylation of p38 MAPK induced by heat shock in PC12m3 cells was significantly greater than that in PC12 parental cells, and a high level of heat-shock-induced neurite outgrowth was observed only in PC12m3 cells. On the other hand, heat-shock-induced JNK activation appeared more quickly and apoptosis started earlier in PC12 parental cells. These findings indicate that short stress induces p38 MAPK and longer stress induces JNK, and that the response of these kinases to heat shock differs depending on cell type.
en-copyright= kn-copyright= en-aut-name=MuraiHiroyasu en-aut-sei=Murai en-aut-mei=Hiroyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiragamiFukumi en-aut-sei=Hiragami en-aut-mei=Fukumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawamuraKenji en-aut-sei=Kawamura en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MotodaHirotoshi en-aut-sei=Motoda en-aut-mei=Hirotoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KoikeYoshihisa en-aut-sei=Koike en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InoueShigeki en-aut-sei=Inoue en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KumagishiKanae en-aut-sei=Kumagishi en-aut-mei=Kanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KanoYoshio en-aut-sei=Kano en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Occupational Therapy, School of Health Science, Kibi International University affil-num=2 en-affil= kn-affil=Department of Physical Therapy, School of Health Science, Kibi International University affil-num=3 en-affil= kn-affil=Department of Physical Therapy, School of Health Science, Kibi International University affil-num=4 en-affil= kn-affil=Department of Physical Therapy, School of Health Science, Kibi International University affil-num=5 en-affil= kn-affil=Department of Occupational Therapy, Faculty of Health and Welfare, Prefectural University of Hiroshima affil-num=6 en-affil= kn-affil=Department of Cellular and Molecular Biology, Research Institute of Health and Welfare, Kibi International University affil-num=7 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Occupational Therapy, School of Health Science, Kibi International University en-keyword=heat shock kn-keyword=heat shock en-keyword=neurite outgrowth kn-keyword=neurite outgrowth en-keyword=p38 MAP kinase kn-keyword=p38 MAP kinase en-keyword=JNK kn-keyword=JNK en-keyword=PC12 mutant cells kn-keyword=PC12 mutant cells END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=1 article-no= start-page=11 end-page=18 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comparison of Capillary Architecture between Slow and Fast Muscles in Rats Using a Confocal Laser Scanning Microscope en-subtitle= kn-subtitle= en-abstract= kn-abstract=The skeletal muscle is classified into 2 types, slow oxidative or fast glycolytic muscle. For further characterization, we investigated the capillary architecture in slow and fast muscles. The rat soleus and extensor digitorum longus (EDL) muscles were used as representatives of slow and fast muscles, respectively. To investigate capillary density, sections of both types of muscle were stained with alkaline phosphatase;the soleus muscle showed more intense reactivity, indicating that it had a denser capillary structure than the EDL muscle. We then injected fluorescent contrast medium into samples of both muscle types for light and confocal-laser microscopic evaluation. The capillary density and capillary-to-fiber ratio were significantly higher, and the course of the capillaries was more tortuous, in the soleus muscle than in the EDL muscle. Capillary coursed more tortuously in the soleus than in the EDL muscle. Succinate dehydrogenase (SDH) activity, an indicator of mitochondrial oxidative capacity, and vascular endothelial growth factor (VEGF) expression were also significantly higher in the soleus muscle. Thus, we conclude that slow oxidative muscle possess a rich capillary structure to provide demanded oxygen, and VEGF might be involved in the formation and/or maintenance of this highly capillarized architecture.
en-copyright= kn-copyright= en-aut-name=MurakamiShinichiro en-aut-sei=Murakami en-aut-mei=Shinichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujinoHidemi en-aut-sei=Fujino en-aut-mei=Hidemi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakedaIsao en-aut-sei=Takeda en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MomotaRyusuke en-aut-sei=Momota en-aut-mei=Ryusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KumagishiKanae en-aut-sei=Kumagishi en-aut-mei=Kanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences affil-num=3 en-affil= kn-affil=Department of Physical Therapy, Himeji Dokkyo University affil-num=4 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=skeletal muscle kn-keyword=skeletal muscle en-keyword=capillaly kn-keyword=capillaly en-keyword=succinate dehydrogenase activity kn-keyword=succinate dehydrogenase activity en-keyword=vascular endothelial growth factor kn-keyword=vascular endothelial growth factor END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=2 article-no= start-page=115 end-page=120 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Biomechanical Evaluation of the Fixation Methods for Transcondylar Fracture of the Humerus:ONI Plate Versus Conventional Plates and Screws en-subtitle= kn-subtitle= en-abstract= kn-abstract=We biomechanically evaluated the bone fixation rigidity of an ONI plate (Group I) during fixation of experimentally created transcondylar humerus fractures in cadaveric elbows, which are the most frequently observed humeral fractures in the elderly, and compared it with the rigidity achieved by 3 conventional fixation methods:an LCP reconstruction plate 3.5 using a locking mechanism (Group II), a conventional reconstruction plate 3.5 (CRP) with a cannulated cancellous screw (Group III), and a CRP with 2 cannulated cancellous screws (CS) in a crisscross orientation (Group IV). In the axial loading test, the mean failure loads were:Group I, 98.9+/-32.6;Group II, 108.5+/-27.2;Group III, 50.0+/-7.5;and Group IV, 34.5+/-12.2 (N). Group I fixations failed at a significantly higher load than those of Groups III and IV (p0.05). In the extension loading test, the mean failure loads were:Group I, 34.0+/-12.4;Group II, 51.0+/-14.8;Group III, 19.3+/-6.0;and Group IV, 14.7+/-3.1 (N). Group IV fixations showed a significantly lower failure load than those of Group I (p0.05). The fixation rigidities against mechanical loading by the ONI plate and LCP plate were comparable. These results suggested that an ONI system might be superior to the CRP and CS method, and comparable to the LCP method in terms of fixation rigidity for distal humerus fractures.
en-copyright= kn-copyright= en-aut-name=ShimamuraYasunori en-aut-sei=Shimamura en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ImataniJunya en-aut-sei=Imatani en-aut-mei=Junya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NodaTomoyuki en-aut-sei=Noda en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HashizumeHiroyuki en-aut-sei=Hashizume en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Departments of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Departments of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Orthopaedic Surgery, Okayama Saiseikai General Hospital affil-num=4 en-affil= kn-affil=Departments of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Orthopaedic Surgery, Kasaoka Daiichi Hospital affil-num=6 en-affil= kn-affil=Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Departments of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=distal humerus kn-keyword=distal humerus en-keyword=fracture kn-keyword=fracture en-keyword=biomechanics kn-keyword=biomechanics en-keyword=internal fixation kn-keyword=internal fixation en-keyword=elderly kn-keyword=elderly END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=3 article-no= start-page=159 end-page=161 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Absence of scalenus anterior muscle. en-subtitle= kn-subtitle= en-abstract= kn-abstract=A rare anomaly of the scalenus muscles is described. In this case, the right scalenus anterior muscle was absent. As a substitute for this muscle, some aberrant muscle slips arose from the lower vertebrae and descended in front of the ventral rami of the lower cervical nerves. These aberrant slips then ran between the ventral rami of the the eighth cervical and first thoracic nerves, and were fused with the right scalenus medius muscle. Thus, the subclavian artery and vein ran in front of the aberrant slips, together with the ventral ramus of the first thoracic nerve. The aberrant muscle slips issued 2 accessory bundles. One bundle ran between the ventral rami of the fourth and fifth cervical nerves and was fused with the scalenus medius muscle; the other bundle ran between the ventral rami of the fifth and sixth cervical nerves and was fused with the scalenus medius muscle.
en-copyright= kn-copyright= en-aut-name=MurakamiShinichiro en-aut-sei=Murakami en-aut-mei=Shinichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HoriuchiKanji en-aut-sei=Horiuchi en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamamotoChugo en-aut-sei=Yamamoto en-aut-mei=Chugo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=scalenus anterior muscle kn-keyword=scalenus anterior muscle en-keyword=scalenus medius muscle kn-keyword=scalenus medius muscle en-keyword=ventral rami of the lower cervical nerves kn-keyword=ventral rami of the lower cervical nerves en-keyword=ventral ramus of the first thoracic nerve kn-keyword=ventral ramus of the first thoracic nerve en-keyword=subclavian artery and vein kn-keyword=subclavian artery and vein END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=3 article-no= start-page=111 end-page=118 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The perineuronal proteoglycan surface coat in the adult rat brain, with special reference to its reactions to Gomori's ammoniacal silver. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The present study showed that many neurons in the adult rat brain possessed a perineuronal sulfated proteoglycan surface coat which reacted to cationic iron colloid and aldehyde fuchsin. This surface coat was stained supravitally with Ehrlich's methylene blue and doubly stained with Ehrlich's methylene blue and aldehyde fuchsin. The surface coat was also stained with Gomori's ammoniacal silver and doubly stained with Gomori's ammoniacal silver and cationic iron colloid. The surface coat was usually expressed together with a nerve cell surface glycoprotein net detectable with lectin Wisteria floribunda agglutinin. These findings indicate that the perineuronal proteoglycan surface coat is identical to Cajal's superficial reticulum and contains some collagenous elements. It was further demonstrated that collagenase digestion erased Gomori's ammoniacal silver impregnation within the perineuronal proteoglycan surface coat.
en-copyright= kn-copyright= en-aut-name=EndoRyutaro en-aut-sei=Endo en-aut-mei=Ryutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiShinichiro en-aut-sei=Murakami en-aut-mei=Shinichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MasudaYu en-aut-sei=Masuda en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=brain kn-keyword=brain en-keyword=extracellular matrix kn-keyword=extracellular matrix en-keyword=perineuronal proteoglycans kn-keyword=perineuronal proteoglycans en-keyword=cell surface glycoproteins kn-keyword=cell surface glycoproteins END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=6 article-no= start-page=243 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Typology of the arteries in the human scalenus region, with special reference to the accessory ascending cervical artery. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The accessory ascending cervical artery (Murakami et al., 1996), which arises from the subclavian artery and ascends between the scalenus anterior and medius muscles, was studied in 87 Japanese adult cadavers (174 sides), with special attention being given to its origin, distribution, and relationship to other arteries at the cervical or scalenus region. In 154 sides (88.5%), the accessory ascending cervical artery was found to originate from the subclavian artery behind the scalenus anterior muscle, and to branch out to the scalenus anterior and medius muscles as well as those entering the 5th and 6th intervertebral foramens along the 6th and 7th cervical nerves. This artery arose independently in 105 sides. The accessory ascending cervical artery issued off or formed a common trunk with the transverse cervical artery and/or costocervical trunk in 49 sides. In cases lacking the accessory ascending cervical artery, it was usually compensated for by the costocervial trunk and/or transverse cervical artery (18 sides). Common trunk formation with the vertebral, internal thoracic, or suprascapular arteries was not observed. The authors suggest that the accessory ascending cervical artery, the transverse cervical artery, and the costocervical trunk should be grouped into one arterial system, a system that may be a remnant of the precostal longitudinal anastomoses of intersegmental arteries of the dorsal aorta behind the scalenus anterior muscle.
en-copyright= kn-copyright= en-aut-name=SuWei-Dong en-aut-sei=Su en-aut-mei=Wei-Dong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=First Clinical Medical College of Harbin Medical University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=accessory ascending cervical artery kn-keyword=accessory ascending cervical artery en-keyword=transverse cervical artery kn-keyword=transverse cervical artery en-keyword=costocervical trunk kn-keyword=costocervical trunk en-keyword=scalenus anterior muscle kn-keyword=scalenus anterior muscle en-keyword=subclavian artery kn-keyword=subclavian artery END start-ver=1.4 cd-journal=joma no-vol=63 cd-vols= no-issue=1 article-no= start-page=65 end-page=69 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Specific Removal of Monocytes from Peripheral Blood of Septic Patients by Polymyxin B-immobilized Filter Column en-subtitle= kn-subtitle= en-abstract= kn-abstract=Lipopolysaccharide (LPS) is one of the major causes of septic shock. The polymyxin B-immobilized filter column (PMX) was developed for the adsorption of endotoxin by direct hemoperfusion and has been used for the treatment of LPS-induced septic shock. In this study, we demonstrated that PMX also specifically bound monocytes from the peripheral blood leukocytes of septic patients by mean of an analysis of bound cells using immunocytochemical and electron microscopic techniques. The specific removal of monocytes from septic patients may produce beneficial effects by reducing the interaction between monocytes and functionally associated cells including vascular endothelial cells. en-copyright= kn-copyright= en-aut-name=NishiboriMasahiro en-aut-sei=Nishibori en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiHide K. en-aut-sei=Takahashi en-aut-mei=Hide K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KatayamaHiroshi en-aut-sei=Katayama en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SaitoShinya en-aut-sei=Saito en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MoritaKiyoshi en-aut-sei=Morita en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Departments of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=septic shock kn-keyword=septic shock en-keyword=polymixin B-immobilized column kn-keyword=polymixin B-immobilized column en-keyword=monocyte kn-keyword=monocyte en-keyword=adsorptive removal kn-keyword=adsorptive removal END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=5 article-no= start-page=245 end-page=253 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=200210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hepatopancreatic arterial ring: bilateral symmetric typology in human celiaco-mesenteric arterial system. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The celiac and mesenteric arterial system including the left gastric, splenic, common hepatic, and superior mesenteric arteries shows various types of origins, courses, ramifications and anastomoses. In order to explain the various expressions of this system, we have proposed a typological model, in which celiacomesenteric arteries develop as paired or bilaterally symmetrical primordial vessels originated from the anterior aspect of the aorta, and these vessels anastomose each other with longitudinal and horizontal pathways. Here, we report 3 unusual cases characterized by arterial rings, formed by the left gastric, left accessory hepatic, proper hepatic, anterior pancreaticoduodenal, and dorsal pancreatic arteries. The dorsal pancreatic and anterior pancreaticoduodenal arteries are located to the right and left of the embryonic pancreas developing in the dorsal mesentery, respectively. Such hepatopancreatic arterial rings simultaneously containing right and left elements can only be explained using our typological model, in which the concept of paired arteries or bilateral symmetry is introduced.
en-copyright= kn-copyright= en-aut-name=KosakaMotohiro en-aut-sei=Kosaka en-aut-mei=Motohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HoriuchiKanji en-aut-sei=Horiuchi en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=arterial variation kn-keyword=arterial variation en-keyword=celiac trunk kn-keyword=celiac trunk en-keyword=superior mesenteric artery kn-keyword=superior mesenteric artery en-keyword=typology kn-keyword=typology en-keyword=bilateral symmetry kn-keyword=bilateral symmetry END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=5 article-no= start-page=267 end-page=269 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=200210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Anterior intercostobrachial nerve penetrating the pectoralis minor or major muscle. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Two previously unknown anomalies of the anterior intercostobrachial nerve were described. In one case, the anterior intercostobrachial nerve penetrated the pectoralis minor muscle. In the other case, it penetrated the pectoralis major muscle. In both cases, the anomalous nerve supplied the skin of the upper arm.
en-copyright= kn-copyright= en-aut-name=MurakamiShinichiro en-aut-sei=Murakami en-aut-mei=Shinichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=anomalies of intercostobrachial nerve kn-keyword=anomalies of intercostobrachial nerve en-keyword=lateral cutaneous branch of the second intercostal nerve kn-keyword=lateral cutaneous branch of the second intercostal nerve en-keyword=pectoralis minor muscle kn-keyword=pectoralis minor muscle en-keyword=pectoralis major muscle kn-keyword=pectoralis major muscle END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=1 article-no= start-page=35 end-page=39 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee.
en-copyright= kn-copyright= en-aut-name=PuJian en-aut-sei=Pu en-aut-mei=Jian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoueHajime en-aut-sei=Inoue en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AsaharaHiroshi en-aut-sei=Asahara en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=mast cell count kn-keyword=mast cell count en-keyword=osteoaethritis kn-keyword=osteoaethritis en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis en-keyword=hydroarthrosis kn-keyword=hydroarthrosis en-keyword=synovial membrance kn-keyword=synovial membrance END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=5 article-no= start-page=239 end-page=244 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199810 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Coexistence of Rare Arteries in the Human Celiaco-Mesenteric System en-subtitle= kn-subtitle= en-abstract= kn-abstract=Some rare anomalies of the celiaco-mesenteric system were observed postmortem in a Japanese adult male: a) The left gastric, common hepatic, splenic and superior mesenteric arteries arose independently from the abdominal aorta. b) The anterior inferior pancreaticoduodenal artery of the superior mesenteric artery issued a hepatic artery which ascended along the anterior surface of the pancreas and gave off the right gastroepiploic, right gastric and cystic arteries. c) The common hepatic artery gave off an anastomosing branch to the superior mesenteric artery. d) The left gastric artery gave off the left accessory hepatic artery. e) The splenic artery issued the accessory middle colic artery. f) The left inferior phrenic artery gave off the esophageal branch. These anomalies are discussed in light of a typological system which we proposed in a previous paper for the celiaco-mesenteric system.
en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MabuchiMasaru en-aut-sei=Mabuchi en-aut-mei=Masaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=GiuvarasteanuLleana en-aut-sei=Giuvarasteanu en-aut-mei=Lleana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KikutaAkio en-aut-sei=Kikuta en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=C.Davila University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=arterial anomaly kn-keyword=arterial anomaly en-keyword=celiac trunk kn-keyword=celiac trunk en-keyword=left gastric artery kn-keyword=left gastric artery en-keyword=hepatic artery kn-keyword=hepatic artery en-keyword=splenic artery kn-keyword=splenic artery en-keyword=intermesenteric anastomosis kn-keyword=intermesenteric anastomosis END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=4 article-no= start-page=189 end-page=196 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Typology of Abdominal Arteries, with Special Reference to Inferior Phrenic Arteries and their Esophageal Branches en-subtitle= kn-subtitle= en-abstract= kn-abstract=Origins and distribution of the human inferior phrenic arteries were studied by dissecting 68 Japanese adult cadavers. The inferior phrenic arteries were usually observed as paired (left and right) vessels. Their origins were summarized as follows: a) the aorta itself (85/138 cases, 61.6%), b) the ventro-visceral arteries (celiaco-mesenteric system of the aorta) including the celiac trunk (39/138 cases, 28.2%) and the left gastric artery (4/138 cases, 2.9%), and c) the latero-visceral arteries (adreno-renal system of the aorta) including the middle adrenal artery (4/138 cases, 2.9%) and the renal artery (6/138 cases, 4.3%). The left and right arteries occasionally originated in common trunk from the aorta, celiaco-mesenteric system or adreno-renal system (22/138 cases, 15.9%). A typological diagram explaining these variations is given. The inferior phrenic arteries, especially the left ones, sometimes issued visceral or esophageal branches. This fact indicates that the inferior phrenic arteries are homologous with the celiac trunk and mesenteric arteries. It is further discussed that the celiac trunk and mesenteric arteries are originally paired vessels, through introduction of our previous typological diagram of the abdominal arteries.
en-copyright= kn-copyright= en-aut-name=PiaoDa Xun en-aut-sei=Piao en-aut-mei=Da Xun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Harbin medical University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=inferior phrenic artery kn-keyword=inferior phrenic artery en-keyword=esophageal branch kn-keyword=esophageal branch en-keyword=typology of abdominal arteries kn-keyword=typology of abdominal arteries END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=4 article-no= start-page=195 end-page=197 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The occurrence of neurons with strongly negatively charged surface coats in mammalian, avian, reptilian, amphibian and piscine brains. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Neurons with strongly negatively charged surface coats were recognized in mammalian, avian, reptilian, amphibian and piscine brains. Many large-sized neurons had strongly negatively charged surface coats in the visual cortex and brain stem of the cow, cat, guinea pig, mouse, quail and parakeet. Such neurons were also seen in the brain stem of the lower vertebrates such as the house lizard, Japanese terrapin, bullfrog, newt, carp and sweetfish.
en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsubouchiMari en-aut-sei=Tsubouchi en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TubouchiYutaka en-aut-sei=Tubouchi en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=central nervous system kn-keyword=central nervous system en-keyword=neurons kn-keyword=neurons en-keyword=negatively charged surface coats kn-keyword=negatively charged surface coats en-keyword=proteoglycans kn-keyword=proteoglycans END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=6 article-no= start-page=327 end-page=334 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The interosseous and lumbrical muscles in the human hand, with special reference to the insertions of the interosseous muscles. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The interosseous and lumbrical muscles in twenty-five hands of Japanese adult cadavers were dissected. The palmar and dorsal interosseous muscles continued, with few exceptions, into the wing tendons. The dorsal interosseous muscles gave off tendons which pierced the transverse laminae or passed deep to the transverse laminae, and attached to the bases of the proximal phalanges. The palmar interosseous muscles seldom had such attachments. The palmar and dorsal interosseous muscles sometimes gave off additional tendons which passed superficial to the transverse laminae and attached to the bases of the proximal phalanges. These latter attachments were typical in the contrahentes muscles. Thus, the present findings suggest that the human dorsal interosseous muscles are composite muscles derived from the dorsal abductor, flexor brevis and contrahens muscles, and that the human palmar interosseous muscles are composite muscles derived from the flexor brevis and contrahens muscles. The lumbrical muscles rarely gave off accessory slips with atavistic attachments to the proximal phalanges.
en-copyright= kn-copyright= en-aut-name=IkebuchiYoshifumi en-aut-sei=Ikebuchi en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=human hand kn-keyword=human hand en-keyword=palmar interosseous muscles kn-keyword=palmar interosseous muscles en-keyword=dorsal interosseous muslcles kn-keyword=dorsal interosseous muslcles en-keyword=lumbrical muscles kn-keyword=lumbrical muscles END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=4 article-no= start-page=215 end-page=226 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=198808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Homology of the adductor pollicis and contrahentes muscles: a study of monkey hands. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The deep palmar muscles in monkey hands were studied. The contrahentes muscles mainly arose from the capitate bone, descended palmar to the deep palmar branch of the ulnar nerve and the palmar metacarpophalangeal nerves, and attached to the proximal phalanges or wing tendons of the second, fourth and fifth fingers. In relation to the deep palmar branch of the ulnar nerve and the palmar metacarpophalangeal nerves, the contrahentes muscles are homologous with the adductor pollicis and flexor indicis radialis muscles. The contrahentes muscles occasionally gave off some accessory slips which blended with the interosseous muscles. These findings suggest that the human adductor pollicis muscle is a well-developed remnant of a contrahens muscle, and that the human interosseous muscles contain some remnant of the contrahentes muscle. In fact, a well-developed remnant of a contrahens muscle was found in the fourth finger of a human hand. It is further considered that the human adductor pollicis muscle contains an element of the interosseous muscle of the thumb.
en-copyright= kn-copyright= en-aut-name=YamamotoChugo en-aut-sei=Yamamoto en-aut-mei=Chugo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=monkey hands kn-keyword=monkey hands en-keyword=contrahentes muscles kn-keyword=contrahentes muscles en-keyword=adductor pollicis muscle kn-keyword=adductor pollicis muscle en-keyword=flexor indicis radialis musle kn-keyword=flexor indicis radialis musle en-keyword=interosseous muscles kn-keyword=interosseous muscles END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=6 article-no= start-page=295 end-page=299 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=199712 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Perineuronal sulfated proteoglycans and cell surface glycoproteins in the visual cortex of adult and newborn cats en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sections of the visual cortex of newborn (1-4 weeks after birth) and adult cats were stained with cationic iron colloid, aldehyde fuchsin or lectins (lectin Vicia villosa, soybean and Wisteria floribunda agglutinins). Many neurons in the adult cat visual cortex contained perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectins. Double staining indicated that some of the lectin-labeled neurons were not stained with cationic iron colloid, and also that some of the cationic iron colloid-stained neurons were not labeled with lectins. The perineuronal sulfated proteoglycans and cell surface glycoproteins developed 3 weeks after birth. In the newborn cats 1-2 weeks after birth, no neurons were reactive to cationic iron colloid, aldehyde fuchsin or lectins. In the newborn cats 34 weeks after birth, it was clearly observed that the cytoplasm of the glial cells closely associated with the neurons containing the perineuronal sulfated proteoglycans showed an intense reaction to cationic iron colloid and aldehyde fuchsin, and that the Golgi complexes of the neurons with cell surface glycoproteins were intensely labeled with lectins. These findings suggest that the perineuronal sulfated proteoglycans are derived from the associated glial cells, and that the cell surface glycoproteins are produced by the associated nerve cells.
en-copyright= kn-copyright= en-aut-name=HitomiSayoko en-aut-sei=Hitomi en-aut-mei=Sayoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SuWei Dong en-aut-sei=Su en-aut-mei=Wei Dong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HongLuo Jia en-aut-sei=Hong en-aut-mei=Luo Jia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=cat brain kn-keyword=cat brain en-keyword=perineuronal sulfated proteoglycans kn-keyword=perineuronal sulfated proteoglycans en-keyword=cell surface glycoproteins kn-keyword=cell surface glycoproteins en-keyword=cationic iron colloid kn-keyword=cationic iron colloid en-keyword=aldehyde fuchsin kn-keyword=aldehyde fuchsin en-keyword=lectin kn-keyword=lectin END start-ver=1.4 cd-journal=joma no-vol=60 cd-vols= no-issue=1 article-no= start-page=59 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=200602 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=In situ preparation of colloidal iron by microwave irradiation for transmission electron microscopy. en-subtitle= kn-subtitle= en-abstract= kn-abstract=We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.
en-copyright= kn-copyright= en-aut-name=NakataniSatoru en-aut-sei=Nakatani en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NaitoIchiro en-aut-sei=Naito en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MomotaRyusuke en-aut-sei=Momota en-aut-mei=Ryusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HinenoyaNoriko en-aut-sei=Hinenoya en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HoriuchiKanji en-aut-sei=Horiuchi en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=colloidal iron kn-keyword=colloidal iron en-keyword=microwave kn-keyword=microwave en-keyword=histochemistry kn-keyword=histochemistry en-keyword=transmission electron microscopy kn-keyword=transmission electron microscopy END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=5 article-no= start-page=242 end-page=253 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199610 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Rat Parathyroid Gland, with Special Reference to Its Blood Vascular Bed, Pericapillary Space and Intercellular Space en-subtitle= kn-subtitle= en-abstract= kn-abstract=The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.
en-copyright= kn-copyright= en-aut-name=TanakaToshihisa en-aut-sei=Tanaka en-aut-mei=Toshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsubouchiMari en-aut-sei=Tsubouchi en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsubouchiYutaka en-aut-sei=Tsubouchi en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=parathyroid gland kn-keyword=parathyroid gland en-keyword=cationic and anionic iron colloid stainings kn-keyword=cationic and anionic iron colloid stainings en-keyword=vascular casting kn-keyword=vascular casting en-keyword=freeze fracture kn-keyword=freeze fracture en-keyword=maceration kn-keyword=maceration END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=5 article-no= start-page=237 end-page=241 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199610 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Perineuronal Sulfated Proteoglycans in the Adult Rat Brain: Histochemical and Electron Microscopic Studies en-subtitle= kn-subtitle= en-abstract= kn-abstract=Neurons of cerebellar nuclei in the rat brain had a marked surface coat which was stained with cationic iron colloid or aldehyde fuchsin. Neurons with a similar surface coat were also noted in the retrosplenial cortex. The surface coat was stained doubly with cationic iron colloid and aldehyde fuchsin. Digestion with hyaluronidase eliminated the stainability of the surface coat to both agents. Combined digestion with chondroitinase ABC, heparitinase and keratanase eliminated the cationic iron colloid staining but did not interfere with the aldehyde fuchsin staining. Electron microscopy of ultrathin sections revealed that the iron particles were deposited in the perineuronal tissue spaces. These findings indicate that the surface coat consists of sulfated proteoglycans which occupy, as the extracellular matrix, the perineuronal tissue spaces. Many neurons in the retrosplenial cortex were labeled with lectin Vicia villosa agglutinin. Double staining revealed that these lectin-labeled neurons are usually reactive to cationic iron colloid. Few neurons in the cerebellar nuclei were labeled with lectin V. villosa agglutinin.
en-copyright= kn-copyright= en-aut-name=TsubouchiYutaka en-aut-sei=Tsubouchi en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsubouchiMari en-aut-sei=Tsubouchi en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HitomiSayoko en-aut-sei=Hitomi en-aut-mei=Sayoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=perineuronal sulfated proteoglycans kn-keyword=perineuronal sulfated proteoglycans en-keyword=cationic iron colloid staining kn-keyword=cationic iron colloid staining en-keyword=aldehyde fuchsin staining kn-keyword=aldehyde fuchsin staining en-keyword=lectin VVA labeling kn-keyword=lectin VVA labeling en-keyword=rat brain kn-keyword=rat brain END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=6 article-no= start-page=313 end-page=317 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Perineuronal Sulfated Proteoglycans, Cell Surface Glycoproteins and Dark Neurons in the Cingulate Cortex of Newborn and Adult Rats en-subtitle= kn-subtitle= en-abstract= kn-abstract=Many neurons in the adult rat cingulate cortex possess perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectin Vicia villosa or soybean agglutinin. The perineuronal sulfated proteoglycans develop three to four weeks after birth. The cell surface glycoproteins develop at earlier stage or two to three weeks after birth. Dark or active neurons begin to appear three to four weeks after birth. These findings indicate that the brain matures after birth or during weaning period.
en-copyright= kn-copyright= en-aut-name=TsubouchiMari en-aut-sei=Tsubouchi en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsubochiYutaka en-aut-sei=Tsubochi en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HitomiSayoko en-aut-sei=Hitomi en-aut-mei=Sayoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=rat brain kn-keyword=rat brain en-keyword=perineuronal sulfated proteoglycans kn-keyword=perineuronal sulfated proteoglycans en-keyword=cell surface glycoproteins kn-keyword=cell surface glycoproteins en-keyword=dark neurons kn-keyword=dark neurons END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=213 end-page=219 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Blood microvascular organization of the nasal-associated lymphoid tissue of the guinea pig: a scanning electron microscopic study of corrosion casts. en-subtitle= kn-subtitle= en-abstract= kn-abstract=It has previously been confirmed that the guinea pig has aggregations of 10-20 lymphoid follicles at the junction of the nasal cavity and the nasopharyngeal duct. The vascular architecture of this nasal-associated lymphoid tissue (NALT) was studied by the corrosion cast/scanning electron microscope method. The NALT was supplied by branches of the inferior nasal artery. These afferent arterial branches gave off arterioles to the follicles and the interfollicular regions, where the arterioles ramified into capillaries. Some of these arterioles reached the subepithelial region to form a single-layer dense capillary network. The subepithelial capillaries gathered into short collecting venules, which in turn drained into high endothelial venules (HEV) in the interfollicular region. The HEV, which also receives tributaries from the follicular and interfollicular capillary plexuses, descended in the interfollicular regions and finally flowed into the efferent veins at the bottom of the NALT. Indentations impressed by high endothelial cells (HEC) were prominent on the surface of the HEV casts, and their frequency was larger in the upper course or segments than in the lower. This suggests that the incidence of HEC in the upper segments is higher than in the lower segments, and these findings are consistent with the hypothesis that some substances which are taken up into the subepithelial capillaries and transported to the venules induce differentiation and maintain of HEVs.
en-copyright= kn-copyright= en-aut-name=OkadaSatoko en-aut-sei=Okada en-aut-mei=Satoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkagiHirofumi en-aut-sei=Akagi en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MasudaYu en-aut-sei=Masuda en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=nasal-associated lymphoid tisse kn-keyword=nasal-associated lymphoid tisse en-keyword=vascular corrosion cast kn-keyword=vascular corrosion cast en-keyword=microvascular architecture kn-keyword=microvascular architecture en-keyword=high endothelial venule kn-keyword=high endothelial venule en-keyword=guinea pig kn-keyword=guinea pig END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=1-2 article-no= start-page=187 end-page=194 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The organization of the elastic tissues of the human and rabbit aorta kn-title=ヒトとウサギの大動脈弾性組織構築 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The organization of the elastic tissues of the human and rabbit aorta was studied by scanning and transmission electron microscopy of tissues treated with 88% formic acid at 45℃. When the tissues were treated with formic acid until they became semitransparent, fixed tissues showed the same organization of the elastic tissues as unfixed tissues. When tissues were treated with formic acid for a short time, the intimal elastic lamina was observed as a plate with numerous small pores or fenestrae, whereas long treatment revealed that the lamina consisted of a network of elastic fibers. The tunica media consisted of concentrically arranged elastic lamellae. There were 50-60 elastic lamellae in humans and about 30 in the rabbit. The elastic fibers, that consisted of elastic microfibrils, ran longitudinally, repeatedly branching and anastomosing, and formed the network of the elastic lamella. Between the adjacent elastic lamellae stretched interlamellar elastic fibers. Thus, the elastic tissues in the aorta formed an integral network, which seems to be highly associated with the aortic function of distributing the pressure applied to the vascular wall. Disruptions of the integrity of the elastic tissue architecture may be responsible for the increased incidence of hypertension with age. en-copyright= kn-copyright= en-aut-name=LiuJing-Jie en-aut-sei=Liu en-aut-mei=Jing-Jie kn-aut-name=劉晶潔 kn-aut-sei=劉 kn-aut-mei=晶潔 aut-affil-num=1 ORCID= en-aut-name=OhtaniOsamu en-aut-sei=Ohtani en-aut-mei=Osamu kn-aut-name=大谷修 kn-aut-sei=大谷 kn-aut-mei=修 aut-affil-num=2 ORCID= en-aut-name=KiutaAkio en-aut-sei=Kiuta en-aut-mei=Akio kn-aut-name=菊田彰夫 kn-aut-sei=菊田 kn-aut-mei=彰夫 aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=4 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name=田口勇仁 kn-aut-sei=田口 kn-aut-mei=勇仁 aut-affil-num=5 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=6 ORCID= en-aut-name=SanoTadashi en-aut-sei=Sano en-aut-mei=Tadashi kn-aut-name=佐能正 kn-aut-sei=佐能 kn-aut-mei=正 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=7 en-affil= kn-affil=医療法人社団光生病院 en-keyword=aorta kn-keyword=aorta en-keyword=elastic tissue kn-keyword=elastic tissue en-keyword=scanning electron microscopy kn-keyword=scanning electron microscopy en-keyword=human kn-keyword=human en-keyword=rabbit kn-keyword=rabbit END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=1-2 article-no= start-page=177 end-page=185 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Three dimensional architecture of the rat femur bone marrow stroma : A scanning electron microscope study combined with fixation and post-ultrasonication method kn-title=ラット骨髄支持組織の三次元的組織構築:固定後超音波振動法による走査電子顕微鏡観察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The three dimensional architecture of the rat femur bone marrow stroma was observed by scanning electron microscopy. The bone marrow was fixed and vibrated by ultrasonication to dislodge the hematopoietic cells embedded in the meshes of the marrow reticulum. The stromal reticulum was exposed, while its three dimensional architecture was preserved.The marrow stromal reticulum consisted of intersinusoidal reticular cells and perivascular reticular cells (adventitial cells), and a small amount of reticular fibers. Two types of meshwork were noted in the bone marrow reticulum. Each mesh of one type was the size of one hematopoietic cell. This meshwork consisted of short and broad or stout cytoplasmic processes. A hematopoietic cell was enwrapped by attenuated cytoplasmic sheaths of the reticular cells and sequestered from the surrounding hematopietic cells. The other type of meshwork was composed of straight running, long and slender filopodia of the reticular cells. Several hematopoietic cells were tightly packed in each mesh of the meshwork of this type, thus presenting a polyhedral appearance. Attenuated adventitial cells enveloped the marrow blood vessels, and were connected with each other by their sheath-like cytoplasmic processes but round, variously sized intercellular spaces remained. The intersinusoidal reticular cells extended their processes to the adventitial cells and the adventitial cells also stretched their processes to the intersiusoidal reticulum. en-copyright= kn-copyright= en-aut-name=SongZheng-Ian en-aut-sei=Song en-aut-mei=Zheng-Ian kn-aut-name=宋振嵐 kn-aut-sei=宋 kn-aut-mei=振嵐 aut-affil-num=1 ORCID= en-aut-name=KikutaAkio en-aut-sei=Kikuta en-aut-mei=Akio kn-aut-name=菊田彰夫 kn-aut-sei=菊田 kn-aut-mei=彰夫 aut-affil-num=2 ORCID= en-aut-name=OhtaniOsamu en-aut-sei=Ohtani en-aut-mei=Osamu kn-aut-name=大谷修 kn-aut-sei=大谷 kn-aut-mei=修 aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=4 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=5 ORCID= en-aut-name=SanoTadashi en-aut-sei=Sano en-aut-mei=Tadashi kn-aut-name=佐能正 kn-aut-sei=佐能 kn-aut-mei=正 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第2解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第2解剖学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第2解剖学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第2解剖学教室 affil-num=6 en-affil= kn-affil=医療法人社団光生病院 en-keyword=bone marrow stroma kn-keyword=bone marrow stroma en-keyword=rat kn-keyword=rat en-keyword=reticulum kn-keyword=reticulum en-keyword=ultrasonication scanning electron microscopy kn-keyword=ultrasonication scanning electron microscopy END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=1-2 article-no= start-page=49 end-page=53 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19930227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Central nervous cells with strongly negatively-charged surface-coats kn-title=強陰性荷電被膜をもつ中枢神経細胞 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Light microscopy of tissue sections stained with cationic iron colloid (pH 1.0-2.0) and nuclear fast red or with this colloid and thionin revealed the presence of numerous neurons with a strong negative-charge or coated with sulfated proteoglycans such as chondroitin sulfates in the adult rat brain. These neurons were distributed mainly in the hippocampal subiculum, zona incerta, cerebral cortex (V lamina), medical cerebellar nuclei and certain other nuclei such as ventral pontine nuclei. In the hippocampal formation, the strongly negatively-charged cells seemed to be identical with the GABAergic inhibitory interneurons reactive to the lectin Vicia villosa agglutinin. The neurons, including the GABAergic Purkinje's cells, of the cerebellar cortex showed no reaction to the cationic iron colloid at pH 1.0-2.0. Many non-GABAergic large neurons of the ventral pontine nuclei were well reactive to the colloid at pH 1.0-2.0. These findings suggest that the cationic iron colloid at pH 1.0-2.0 mainly stains some subsets of GABA-ergic neurons, and additionally stains some non-GABAergic interneurons projecting long association or commissural fibers. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name=田口勇仁 kn-aut-sei=田口 kn-aut-mei=勇仁 aut-affil-num=2 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第二解剖学教室 en-keyword=Cationic iron colloid staining kn-keyword=Cationic iron colloid staining en-keyword=central nervous system kn-keyword=central nervous system en-keyword=neurons kn-keyword=neurons en-keyword=sulfated proteoglycans kn-keyword=sulfated proteoglycans END start-ver=1.4 cd-journal=joma no-vol=108 cd-vols= no-issue=9-10 article-no= start-page=303 end-page=310 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19961031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Dark and light neurons in the central nervous system Light and electron microscopic study of the mouse brain kn-title=暗調と明調神経細胞マウスの脳における光顕と電顕所見 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The cerebral cortex, cerebellar cortex and hippocampus of adult mice were studied by light and electron microscopy of tissue sections. Dark neurons were well stained with nuclear fast red, and clearly distinguishable from light neurons. The dark neurons were classified into active and resting types. Active types were characterized by their well developed Golgi's complexes. Resting types contained poorly developed Golgi's complexes. These findings indicate that active dark neurons are converted into the light neurons via resting dark neurons. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=2 ORCID= en-aut-name=YamanaSeizo en-aut-sei=Yamana en-aut-mei=Seizo kn-aut-name=山名征三 kn-aut-sei=山名 kn-aut-mei=征三 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=3 en-affil= kn-affil=東広島記念病院（附）広島リウマチ・膠原病センター en-keyword=Dark neurons kn-keyword=Dark neurons en-keyword=light neurons kn-keyword=light neurons en-keyword=central nervous system kn-keyword=central nervous system en-keyword=mouse kn-keyword=mouse END start-ver=1.4 cd-journal=joma no-vol=108 cd-vols= no-issue=9-10 article-no= start-page=293 end-page=296 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19961031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Tannic acid as a tissue fixative, with special reference to mixed use with glutaraldehyde and paraformaldehyde kn-title=組織固定剤としてのタンニン酸，特にグルタールアルデヒドとパラホルムアルデヒドとの混用 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Tannic acid mixed with glutaraldehyde or paraformaldehyde is a strong fixative, which can precipitate amino acids and oligopeptides such as arginine and glutathione. Tissue specimens fixed with this tannin-aldehyde mixture are useful for histochemical studies, including lectin labeling, immunological staining and tissue enzyme digestion. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=PiaoDa Xun en-aut-sei=Piao en-aut-mei=Da Xun kn-aut-name=朴大 kn-aut-sei=朴 kn-aut-mei=大 aut-affil-num=2 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=3 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name=西田圭一郎 kn-aut-sei=西田 kn-aut-mei=圭一郎 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第二解剖学教室 en-keyword=Tannic acid kn-keyword=Tannic acid en-keyword=paraformaldehyde kn-keyword=paraformaldehyde en-keyword=glutaraldehyde kn-keyword=glutaraldehyde en-keyword=tissue fixation kn-keyword=tissue fixation END start-ver=1.4 cd-journal=joma no-vol=107 cd-vols= no-issue=11-12 article-no= start-page=235 end-page=242 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960229 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sleep restores the ratio of dark neurons to light neuros in the central nervous system kn-title=睡眠は中枢神経の暗調細胞を明調細胞に復する en-subtitle= kn-subtitle= en-abstract= kn-abstract=Blocks of the hippocampal subiculum, hippocampus, intracerebellar nuclei, cerebellar cortex and visual cortex were isolated from adult mice in the evening. at midnight or in the next morning. They were embedded in paraffin, cut into sections, stained with cationic iron colloid or aldehyde fuchsin and counter-stained with nuclear fast red or carbol-thionin. Specimens prepared at midnight contained a markedly increased number of dark neurons. In the cerebel-lar cortex, 50% or more of Punkinje cells were daek at midnight. The dark neurons, except for Purkinje cells, were usually provided with extracellular sulfated proteoglycans reactive to cationic iron colloid or aldehyde fuchsin. The specimens, including those of intracerebellar nuclei and cerebellar cortex, prepared in the next morning (or when the animals were sleeping) contained few dark neurons. These findings suggest that the dark neurons are exhausted or tired cells, which are restored to normal or light cells with sleep (resting of neurons), and that they are neither porly fixed nor degenerative cells. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 en-keyword=Central nervous system kn-keyword=Central nervous system en-keyword=dark neurons kn-keyword=dark neurons en-keyword=clear or light neurons kn-keyword=clear or light neurons en-keyword=sleep kn-keyword=sleep en-keyword=sukfated proteoglycans kn-keyword=sukfated proteoglycans END start-ver=1.4 cd-journal=joma no-vol=107 cd-vols= no-issue=9-10 article-no= start-page=219 end-page=226 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19951031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Typology of the human coeliac, left gastric, splenic, hepatic, superior mesenteric, inferior mesenteric and inferior phrenic arteries kn-title=ヒト腹腔, 左胃, 脾, 肝, 上腸間膜, 下腸間膜, 下横隔動脈群の類型解剖学 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Four cases of dissected human coeliac and other mesenterial arteries are presented. Typologic discussion of these cases indicates that the arterial system of human mesentery vessels basically consists of left gastric, splenic, (common) hepatic, superior mesenteric and inferior mesenteric arteries, and that these arteries, like the inferior phrenic and inferior rectal arteries, are originally paired vessels (left and right). The left gastric, (common) hepatic and superior mesenteric arteries are thus derived from the right-sided vessels, while the splenic and inferior mesenteric arteries arise from the left-sided vessels. The aberrant acces-sory colica media artery is a typical left-sided vessed. The celiac trunk is also left-sided vessed. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=2 ORCID= en-aut-name=PiaoDa xun en-aut-sei=Piao en-aut-mei=Da xun kn-aut-name=朴大 kn-aut-sei=朴 kn-aut-mei=大 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第二解剖学教室 en-keyword=Typology kn-keyword=Typology en-keyword=left gastric artery kn-keyword=left gastric artery en-keyword=splenic artery kn-keyword=splenic artery en-keyword=superior mesenteric artery kn-keyword=superior mesenteric artery en-keyword=inferior mesenteric artery kn-keyword=inferior mesenteric artery END start-ver=1.4 cd-journal=joma no-vol=108 cd-vols= no-issue=3-6 article-no= start-page=145 end-page=148 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960629 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Artery ascending between the scalenus anterior and medius muscles. Accessory ascending cervical artery kn-title=斜角筋隙を上行する動脈―副上行頚動脈 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Dissection of 25 adult Japanese cadavers revealed an artery that consistently arose from the subclavian artery and ascended between the scalenus anterior and medius muscles. This accessory ascending cervical artery, when developed, issued the dorsal scapular artery which penetrated the scalenus medius muscle. Typological discussion suggested that the accessory ascending cervical artery was a remnant of the longitudinal anastomoses along the embryonic intersegmental arteries. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=HitomiSayoko en-aut-sei=Hitomi en-aut-mei=Sayoko kn-aut-name=人見さよ子 kn-aut-sei=人見 kn-aut-mei=さよ子 aut-affil-num=2 ORCID= en-aut-name=SatoTohru en-aut-sei=Sato en-aut-mei=Tohru kn-aut-name=佐藤徹 kn-aut-sei=佐藤 kn-aut-mei=徹 aut-affil-num=3 ORCID= en-aut-name=PiaoDa Xun en-aut-sei=Piao en-aut-mei=Da Xun kn-aut-name=朴大 kn-aut-sei=朴 kn-aut-mei=大 aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=5 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name=田口勇仁 kn-aut-sei=田口 kn-aut-mei=勇仁 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第二解剖学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第二解剖学教室 en-keyword=Accessory ascending cervical artery kn-keyword=Accessory ascending cervical artery en-keyword=dorsal scapular artery kn-keyword=dorsal scapular artery en-keyword=vertebral artery kn-keyword=vertebral artery en-keyword=subclavian artery kn-keyword=subclavian artery en-keyword=intersegmental artery kn-keyword=intersegmental artery END start-ver=1.4 cd-journal=joma no-vol=109 cd-vols= no-issue=7-12 article-no= start-page=151 end-page=156 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19971225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Physical development of tissue-reacted cationic colloid with protein silver and gold chloride kn-title=組織反応陽性荷電鉄コロンドのプロテイン銀と塩化金による現像 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rat brain sections were treated with fine granular cationic iron colloid, which was prepared by boilling ferric chloride with hydrazine hydrate and cacodylic acid (Murakami et al., 1986). The sections were then treated with ferrocyanide and HCl for Prussian blue reaction or with protein silver and gold chloride for physical development. Physical development had an advantage over Prussian blue reaction in that it produces clear and highly contrasted images under a light microscope. Thus, the perineuronal sulfated proteoglycans and the fine processes of the nerve cells were clearly demonstrated by physical development. en-copyright= kn-copyright= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=1 ORCID= en-aut-name=SuWei Dong en-aut-sei=Su en-aut-mei=Wei Dong kn-aut-name=蘇衛東 kn-aut-sei=蘇 kn-aut-mei=衛東 aut-affil-num=2 ORCID= en-aut-name=HongLuo Jia en-aut-sei=Hong en-aut-mei=Luo Jia kn-aut-name=洪珞珈 kn-aut-sei=洪 kn-aut-mei=珞珈 aut-affil-num=3 ORCID= en-aut-name=PiaoDa Xun en-aut-sei=Piao en-aut-mei=Da Xun kn-aut-name=朴大 kn-aut-sei=朴 kn-aut-mei=大 aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=5 ORCID= en-aut-name=SeoKenji en-aut-sei=Seo en-aut-mei=Kenji kn-aut-name=瀬尾憲治 kn-aut-sei=瀬尾 kn-aut-mei=憲治 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部解剖学第二講座 affil-num=2 en-affil= kn-affil=岡山大学医学部解剖学第二講座 affil-num=3 en-affil= kn-affil=岡山大学医学部解剖学第二講座 affil-num=4 en-affil= kn-affil=岡山大学医学部解剖学第二講座 affil-num=5 en-affil= kn-affil=岡山大学医学部解剖学第二講座 affil-num=6 en-affil= kn-affil=健照会セオ病院 en-keyword=Cationic iron colloid kn-keyword=Cationic iron colloid en-keyword=physical development kn-keyword=physical development en-keyword=Prussian blue reaction kn-keyword=Prussian blue reaction en-keyword=protein silver kn-keyword=protein silver en-keyword=gold chloride kn-keyword=gold chloride en-keyword=Bodian's silver impregnation kn-keyword=Bodian's silver impregnation END start-ver=1.4 cd-journal=joma no-vol=113 cd-vols= no-issue=1 article-no= start-page=27 end-page=31 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20010428 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A typical highest thoracic artery (A. thoracica suprema) -a case report- kn-title=最上胸動脈，典型的と思われる1例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A typical higest thoracic artery with anterior, posterior, ascending and descending branches was reported. Discussion suggests that this artery, when developed, gives rise to the internal thoracic artery descening lateral to the scalenus anterior muscle, transverse cervical artery, descending scapular artery, lateral thoracic artery or subscapular artery. en-copyright= kn-copyright= en-aut-name=MurakamiShinichirou en-aut-sei=Murakami en-aut-mei=Shinichirou kn-aut-name=村上慎一郎 kn-aut-sei=村上 kn-aut-mei=慎一郎 aut-affil-num=1 ORCID= en-aut-name=MatsuokaHiroaki en-aut-sei=Matsuoka en-aut-mei=Hiroaki kn-aut-name=松岡宏明 kn-aut-sei=松岡 kn-aut-mei=宏明 aut-affil-num=2 ORCID= en-aut-name=FuyamaYasuhiro en-aut-sei=Fuyama en-aut-mei=Yasuhiro kn-aut-name=普山康弘 kn-aut-sei=普山 kn-aut-mei=康弘 aut-affil-num=3 ORCID= en-aut-name=TaguchiTakehito en-aut-sei=Taguchi en-aut-mei=Takehito kn-aut-name=田口勇仁 kn-aut-sei=田口 kn-aut-mei=勇仁 aut-affil-num=4 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=5 ORCID= en-aut-name=MurakamiTakuro en-aut-sei=Murakami en-aut-mei=Takuro kn-aut-name=村上宅郎 kn-aut-sei=村上 kn-aut-mei=宅郎 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部医学科解剖学第二講座 affil-num=2 en-affil= kn-affil=岡山大学医学部医学科解剖学第三講座 affil-num=3 en-affil= kn-affil=岡山大学医学部医学科解剖学第二講座 affil-num=4 en-affil= kn-affil=岡山大学医学部保健学科放射線技術科学専攻 affil-num=5 en-affil= kn-affil=岡山大学医学部医学科解剖学第二講座 affil-num=6 en-affil= kn-affil=岡山大学医学部医学科解剖学第二講座 en-keyword=A. thoracia suprema kn-keyword=A. thoracia suprema en-keyword=A. transversa colli kn-keyword=A. transversa colli en-keyword=A. scapularis descendens kn-keyword=A. scapularis descendens en-keyword=A. thoracica lateralis kn-keyword=A. thoracica lateralis en-keyword=A. subscapularis kn-keyword=A. subscapularis END start-ver=1.4 cd-journal=joma no-vol=117 cd-vols= no-issue=1 article-no= start-page=35 end-page=39 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050520 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=複合糖質を可視化する en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯学総合研究科人体構成学 en-keyword=複合糖質 kn-keyword=複合糖質 en-keyword=組織化学 kn-keyword=組織化学 en-keyword=プロテオグリカン kn-keyword=プロテオグリカン en-keyword=電子顕微鏡 kn-keyword=電子顕微鏡 en-keyword=光学顕微鏡 kn-keyword=光学顕微鏡 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19840331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ラット蔓状静脈叢 精巣動脈系の微小血管構築,鋳型の走査電子顕微鏡観察 kn-title=Microvascular architecture of the pampiniform plexus-testicular artery system in the rat. A scanning electron microscope study of corrosion casts. en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=大塚愛二 kn-aut-sei=大塚 kn-aut-mei=愛二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END