Three outbreaks and many isolated cases of enterohemorrhagic Escherichia coli O157:H7 occurred in 1996 and 1997 in Okayama Prefecture, Japan. In an attempt to investigate the route of these infections, the strains isolated from the 3 outbreaks (total 33 strains) and 15 isolated cases (total 15 strains) were investigated using random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE). In addition, 10 strains from an outbreak in Tojo Cho, Hiroshima Prefecture (June 1996), 2 strains from the particular types of meat in Kochi Prefecture, and 42 strains isolated from bovine feces in a farm in Okayama Prefecture were also investigated in the same manner. PFGE was much more useful than RAPD for molecular typing of the clinical isolates, in that it allowed us to classify them into 10 PFGE groups. We noted that the strains differed according to the time and place of the outbreaks (or isolated cases). This indicates that O157:H7 infections in Okayama Prefecture were caused by different strains (although some cases were aggravated by the same strains as were found in other areas). The isolates from bovine feces were classified into 5 groups by PFGE profiles, but none of them were identical to those of the clinical isolates.
en-copyright= kn-copyright= en-aut-name=FunamoriYuka en-aut-sei=Funamori en-aut-mei=Yuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujinagaYukako en-aut-sei=Fujinaga en-aut-mei=Yukako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokotaKenji en-aut-sei=Yokota en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=InoueKaoru en-aut-sei=Inoue en-aut-mei=Kaoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HiraiYoshikazu en-aut-sei=Hirai en-aut-mei=Yoshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OgumaKeiji en-aut-sei=Oguma en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KiraShohei en-aut-sei=Kira en-aut-mei=Shohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Jichi Medical School, Tochigi affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=molecular epidemiology kn-keyword=molecular epidemiology en-keyword=enterohemorrhagic Escherichia coli O157: H7 kn-keyword=enterohemorrhagic Escherichia coli O157: H7 en-keyword=pulsed field gel electrophoresis kn-keyword=pulsed field gel electrophoresis en-keyword=random amplification of polymorphic DNA kn-keyword=random amplification of polymorphic DNA END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=1 article-no= start-page=41 end-page=48 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=199802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antibody and Cytokine Responses in Helicobacter pylori-Infected Various Mouse Strains en-subtitle= kn-subtitle= en-abstract= kn-abstract=Helicobacter pylori (H. pylori) infection in the stomach is etiologically closely associated with chronic active gastritis, peptic ulcer, gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. In this study, we examined the antibody responses and cytokine profiles of three strains of mice (BALB/c, C3H/He, and C57BL/6) infected with H. pylori. Following this, correlations between host-immune reactions and intensity of inflammation were analyzed. H. pylori (ATCC43504) was intragastrically administered once a week to the mice from 4 weeks of age, and they were sacrificed at the ages of 4 and 7 months. In these mice, we examined the histology of the stomach, antibody titers against H. pylori, and serum levels of cytokines (IL-4, IL-10, TNF-alpha, IL-2 and Interferon-gamma). In BALB/c mice, inflammation of the stomach was minimal. Inflammation was observed in 63.6% of C57BL/6 mice and 33.3% of C3h/He mice. In C57BL/6 and C3H/He mice, all the cytokines tended to increase. In contrast, BALB/c mice were inactive in cytokine production except for IL-2. Two C3H/He mice developed severe inflammation with lymph follicles; one showed a response largely typical of Th-1, and the other showed a response largely typical of Th-2. Although a definite correlation was not shown between Th-1/Th-2 response evaluated by cytokine production and intensity of inflammation, it appears that in H. pylori-induced inflammation both cell-mediated (Th-1) and humoral (Th-2) immunity play a role in pathogenesis.
en-copyright= kn-copyright= en-aut-name=DeyAshoka en-aut-sei=Dey en-aut-mei=Ashoka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YokotaKenji en-aut-sei=Yokota en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiKeita en-aut-sei=Kobayashi en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OgumaKeiji en-aut-sei=Oguma en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HiraiYoshikazu en-aut-sei=Hirai en-aut-mei=Yoshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=Helicobacter pylori kn-keyword=Helicobacter pylori en-keyword=cytokine kn-keyword=cytokine en-keyword=humoral immunity kn-keyword=humoral immunity en-keyword=cell-mediated immunity kn-keyword=cell-mediated immunity en-keyword=gastritis kn-keyword=gastritis END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=205 end-page=211 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199508 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lipid profiles of Helicobacter pylori and Helicobacter mustelae grown in serum-supplemented and serum-free media. en-subtitle= kn-subtitle= en-abstract= kn-abstract=Many of Helicobacter species have been found to have novel cholesteryl glucosides (CGs). To study the biosynthetic mechanism of CGs, the lipid profiles of H. pylori and H. mustelae grown in serum-supplemented and cholesterol-restricted serum-free media were investigated. In contrast to the serum-supplemented state, helicobacters had less CGs in the serum-free state; a trace amount of CGs and no CG was detected in H. pylori and H. mustelae, respectively. The proportion of total and individual phospholipid also showed significant alteration. Unknown lipids which did not contain phosphate and sugar were detected in the serum-free state, but not in the serum-supplemented state. The CGs were found to be distributed mainly in the membrane fractions, and one of the unknown lipids was found exclusively in the cytosol fraction. Based on these data, it is apparent that the CGs of helicobacters are synthesized by de novo uptake of cholesterol from the media. The unknown lipids detected in the serum-free state may be storage lipids, appearing in response to depletion of nutrients, especially cholesterol, or other factors in the media.
en-copyright= kn-copyright= en-aut-name=HaqueMahmudul en-aut-sei=Haque en-aut-mei=Mahmudul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiraiYoshikazu en-aut-sei=Hirai en-aut-mei=Yoshikazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokotaKenji en-aut-sei=Yokota en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OgumaKeiji en-aut-sei=Oguma en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=Helicobacter kn-keyword=Helicobacter en-keyword=steryl glycoside kn-keyword=steryl glycoside en-keyword=cholesteryl glucoside kn-keyword=cholesteryl glucoside END start-ver=1.4 cd-journal=joma no-vol=113 cd-vols= no-issue=1 article-no= start-page=113 end-page=114 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20010428 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Š´õÇV–@ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=¬ŒFœ¨“ñ kn-aut-sei=¬ŒF kn-aut-mei=œ¨“ñ aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=‰ªŽR‘åŠwˆãŠw•”׋ۊwuÀ END start-ver=1.4 cd-journal=joma no-vol=113 cd-vols= no-issue=1 article-no= start-page=101 end-page=106 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20010428 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Š´õÇ‚ÆŠà en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=‰Á“¡é”V kn-aut-sei=‰Á“¡ kn-aut-mei=é”V aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=¬ŒFœ¨“ñ kn-aut-sei=¬ŒF kn-aut-mei=œ¨“ñ aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=‰ªŽR‘åŠwˆãŠw•”•a‘Ô•ªŽq¶•¨ŠwuÀ affil-num=2 en-affil= kn-affil=‰ªŽR‘åŠwˆãŠw•”׋ۊwuÀ en-keyword=CŒ^ŠÌ‰ŠƒEƒBƒ‹ƒX kn-keyword=CŒ^ŠÌ‰ŠƒEƒBƒ‹ƒX en-keyword=EBƒEƒBƒ‹ƒX kn-keyword=EBƒEƒBƒ‹ƒX en-keyword=Helicobacter pylori kn-keyword=Helicobacter pylori END start-ver=1.4 cd-journal=joma no-vol=118 cd-vols= no-issue=3 article-no= start-page=209 end-page=213 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=ƒ{ƒcƒŠƒkƒXCŒ^‚ÆDŒ^_Œo“Å‘f‚ðŽx”z‚·‚éƒoƒNƒeƒŠƒIƒtƒ@[ƒW‚̈â“`Žq‰ðÍ‚Æ—nŒ´‰»‚Ì•ªŽq‹@\ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=ãŒû‹`•F kn-aut-sei=ãŒû kn-aut-mei=‹`•F aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=—Ñ“N–ç kn-aut-sei=—Ñ kn-aut-mei=“N–ç aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=•ìŒ° kn-aut-sei=•ì kn-aut-mei=Œ° aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=’†ŽRŒb‰î kn-aut-sei=’†ŽR kn-aut-mei=Œb‰î aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=‘哇Œ’Žu˜Y kn-aut-sei=‘哇 kn-aut-mei=Œ’Žu˜Y aut-affil-num=5 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=“¡‰i—R‰ÀŽq kn-aut-sei=“¡‰i kn-aut-mei=—R‰ÀŽq aut-affil-num=6 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=‘å¼^ kn-aut-sei=‘å¼ kn-aut-mei=^ aut-affil-num=7 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=‘å’؉hˆê kn-aut-sei=‘å’Ø kn-aut-mei=‰hˆê aut-affil-num=8 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=•ž•”³•½ kn-aut-sei=•ž•” kn-aut-mei=³•½ aut-affil-num=9 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=¬ŒFœ¨“ñ kn-aut-sei=¬ŒF kn-aut-mei=œ¨“ñ aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=‰ªŽR‘åŠw‘åŠw‰@ˆãŽ•–òŠw‘‡Œ¤‹†‰È@•aŒ´×‹ÛŠw affil-num=2 en-affil= kn-affil=‹{è‘åŠwƒtƒƒ“ƒeƒBƒA‰ÈŠwŽÀŒ±‘‡ƒZƒ“ƒ^[@¶–½‰ÈŠwŒ¤‹†•”–嶖½ŠÂ‹«‰ÈŠw•ª–ì affil-num=3 en-affil= kn-affil=“Þ—Çæ’[‰ÈŠw‹Zp‘åŠw‰@‘åŠwî•ñ‰ÈŠwŒ¤‹†‰È@”äŠrƒQƒmƒ€ affil-num=4 en-affil= kn-affil=‹{è‘åŠwˆãŠw•”ˆãŠw‰È@Š´õÇŠw affil-num=5 en-affil= kn-affil=–k—¢‘åŠw¶–½‰ÈŠwŒ¤‹†Š affil-num=6 en-affil= kn-affil=‘åã‘åŠw”÷¶•¨•aŒ¤‹†Š•‘®Š´õÇ‘ÛŒ¤‹†ƒZƒ“ƒ^[ affil-num=7 en-affil= kn-affil=‘—§Š´õÇŒ¤‹†Š@׋ۑæˆê•” affil-num=8 en-affil= kn-affil=“Œ‹ž‘åŠw•ªŽq×–E¶•¨ŠwŒ¤‹†Š@•ªŽqî•ñE§Œä affil-num=9 en-affil= kn-affil=–k—¢‘åŠw¶–½‰ÈŠwŒ¤‹†Š affil-num=10 en-affil= kn-affil=‰ªŽR‘åŠw‘åŠw‰@ˆãŽ•–òŠw‘‡Œ¤‹†‰È@•aŒ´×‹ÛŠw en-keyword=ƒoƒNƒeƒŠƒIƒtƒ@[ƒW kn-keyword=ƒoƒNƒeƒŠƒIƒtƒ@[ƒW en-keyword=ƒ{ƒcƒŠƒkƒX“Å‘f kn-keyword=ƒ{ƒcƒŠƒkƒX“Å‘f en-keyword=‘}“ü”z—ñ kn-keyword=‘}“ü”z—ñ en-keyword=ƒvƒ‰ƒXƒ~ƒh kn-keyword=ƒvƒ‰ƒXƒ~ƒh en-keyword=ƒvƒƒtƒ@[ƒW kn-keyword=ƒvƒƒtƒ@[ƒW END