ID | 52343 |
FullText URL | |
Author |
Yamamoto, Sumiharu
Okazaki, Mikio
Yamane, Masaomi
Kaken ID
researchmap
Miyoshi, Kentaro
Kaken ID
Otani, Shinji
Kakishita, Tomokazu
Yoshida, Osamu
Waki, Naohisa
Toyooka, Shinichi
Sano, Yoshifumi
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Abstract | Background: Although lung transplantation from donation after cardiac death (DCD), especially uncontrolled DCD, is limited by warm ischemic periods, the molecular mechanism of warm ischemia-reperfusion-injury (IRI) has not been well elucidated. The purpose of this study was to clarify the particular longitudinal mechanisms of molecular factors involved in warm IRI.
Methods: Cold ischemic-time (CIT)-group lungs were retrieved and subjected to 3-h of cold preservation, whereas warm ischemic-time (WIT)-group lungs were retrieved after 3-h of warm ischemia. Orthotopic rat lung transplantation was performed and the grafts were reperfused for 1 or 4-h. The graft functions, gene expression, and activation of inflammatory molecules in the grafts were analyzed. Exhaled-carbon-monoxide-concentration (ExCO-C) was measured during reperfusion.
Results: Only the WIT-group showed obvious primary graft dysfunction at 1-h reperfusion, but the graft function was recovered during 4-h reperfusion. Most of pro-inflammatory cytokines and stress-induced molecules showed different expression and activation patterns between CIT and WIT groups. In the WIT-group, the expressions of anti-inflammatory molecules, IL-10 and HO-1, were significantly increased at 1-h reperfusion compared to the CIT-group, and these high levels were maintained through 4-h reperfusion. Furthermore, ExCO-C levels in the WIT-group increased immediately after reperfusion compared to the CIT-group.
Conclusions: This study indicates that warm IRI may involve a different mechanism than cold IRI and anti-inflammatory pathways may play important roles in the graft recovery after lung transplantation from uncontrolled DCD.
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Keywords | Lung transplantation
Donation after cardiac death
Ischemia reperfusion injury
Warm ischemia
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Published Date | 2012-03
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Publication Title |
Transplant Immunology
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Volume | volume26
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Issue | issue2-3
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Start Page | 133
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End Page | 139
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ISSN | 0966-3274
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Content Type |
Journal Article
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Official Url | http://www.sciencedirect.com/science/article/pii/S0966327411001298
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Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/52245
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language |
English
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Copyright Holders | (C) 2011 Elsevier B.V. All rights reserved.
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File Version | author
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Refereed |
True
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DOI | |
Web of Science KeyUT |