| FullText URL | fulltext.pdf |
|---|---|
| Author | Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori| |
| Keywords | Biomineralization Diatom Silica Transcriptome Proteome |
| Note | This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1.| |
| Published Date | 2020-06-03 |
| Publication Title | Marine Biotechnology |
| Publisher | Springer |
| ISSN | 1436-2228 |
| NCID | AA11357643 |
| Content Type | Journal Article |
| language | French |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 32488507 |
| DOI | 10.1007/s10126-020-09976-1 |
| Web of Science KeyUT | 000537432000001 |
| Related Url | isVersionOf https://doi.org/10.1007/s10126-020-09976-1 |
| Title Alternative | A comparative gene analysis reveals a diatom-specific SET domain protein family |
|---|---|
| FullText URL | srfa_110_001_006.pdf |
| Author | Nemoto, Michiko| |
| Abstract | The silica cell walls of diatoms, which exhibit species-spe-cific micro- and nano- patterned structures are promising candidates for applications in nanotechnology. Previous studies revealed a number of silica cell wall-associated proteins involved in silica formation. However, molecular biological analyses toward understanding of diatom cell wall formation have been mostly limited to model diatom species and general silica formation process in diatoms is still incompletely understood. In this study, to gain a compre-hensive insight into diatom silica biomineralization, tran-scriptome data of three diatom species, Nitzschia palea, Achnanthes kuwaitensis and Pseudoleyanella lunata, were newly developed. The reads obtained from RNA sequencing were assembled into 31,946, 60,767 and 38,314 unique transcripts for N. palea, A. kuwaitensis and P. lunata, respectively. In order to identify the diatom-specific genes, three transcriptome data sets developed in this study and the protein-coding gene sets of five genome-sequenced diatoms were compared. The proteins shared only by eight diatom species that are predicted to possess an endoplasmic reticulum (ER)-targeting signal peptide were selected for further analyses. These include proteins showing homology to silicanin-1, a recently reported diatom-specific protein involved in silica formation, as well as a number of SET domain proteins. The SET domain proteins might be novel diatom-specific family of methyltransferases that may reg-ulate the function of silica formation related proteins or long chain polyamines. The genes encoding the diatom-specific SET domain proteins identified in this study, which were shown to respond to silicon were suggested to be implicated in silica biomineralization. |
| Keywords | Biomineralization Diatom Silica Protein |
| Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
| Published Date | 2021-02-01 |
| Volume | volume110 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 2186-7755 |
| language | Japanese |
| File Version | publisher |
| NAID | 120006955817 |
| Author | Oshima, Kenshiro| Hattori, Masahira| Shimizu, Hitomi| Fukuda, Koji| Nemoto, Michiko| Inagaki, Kenji| Tamura, Takashi| |
|---|---|
| Published Date | 2015-07-09 |
| Publication Title | Genome Announcements |
| Volume | volume3 |
| Issue | issue4 |
| Content Type | Journal Article |
| Author | Tamura, Takashi| Tsunekawa, Naoki| Nemoto, Michiko| Inagaki, Kenji| Hirano, Toshiyuki| Sato, Fumitoshi| |
|---|---|
| Published Date | 2016-01-28 |
| Publication Title | Scientific reports |
| Volume | volume6 |
| Content Type | Journal Article |
| Author | Okada, Koki| Morimoto, Yu| Shiraishi, Yukine| Tamura, Takashi| Mayama, Shigeki| Kadono, Takashi| Adachi, Masao| Ifuku, Kentaro| Nemoto, Michiko| |
|---|---|
| Keywords | Diatom Genetic transformation Nitzschia Multi-pulse electroporation |
| Note | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10126-023-10273-w| This fulltext file will be available in Dec. 2024.| |
| Published Date | 2023-12-10 |
| Publication Title | Marine Biotechnology |
| Volume | volume25 |
| Issue | issue6 |
| Publisher | Springer Science and Business Media LLC |
| Start Page | 1208 |
| End Page | 1219 |
| ISSN | 1436-2228 |
| NCID | AA11357643 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023 |
| File Version | author |
| PubMed ID | 38071657 |
| DOI | 10.1007/s10126-023-10273-w |
| Web of Science KeyUT | 001120097200001 |
| Related Url | isVersionOf https://doi.org/10.1007/s10126-023-10273-w |
