FullText URL fulltext.pdf
Author Ohashi, Hideki| Nishida, Keiichiro| Nasu, Yoshihisa| Saiga, Kenta| Nakahara, Ryuichi| Horita, Masahiro| Okita, Shunji| Ozaki, Toshifumi|
Keywords lesser toe metatarsophalangeal joint rheumatoid arthritis radiographic measurement grading system
Published Date 2021-07-15
Publication Title International Journal of Environmental Research and Public Health
Volume volume18
Issue issue14
Publisher MDPI
Start Page 7520
ISSN 1660-4601
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2021 by the authors.
File Version publisher
PubMed ID 34299970
DOI 10.3390/ijerph18147520
Web of Science KeyUT 000676598200001
Related Url isVersionOf https://doi.org/10.3390/ijerph18147520
Author Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
Published Date 2005-5
Publication Title Arthritis & Rheumatism
Volume volume52
Issue issue5
Content Type Journal Article
JaLCDOI 10.18926/AMO/32855
FullText URL fulltext.pdf
Author Shimamura, Yasunori| Nishida, Keiichiro| Imatani, Junya| Noda, Tomoyuki| Hashizume, Hiroyuki| Ohtsuka, Aiji| Ozaki, Toshifumi|
Abstract

We biomechanically evaluated the bone fixation rigidity of an ONI plate (Group I) during fixation of experimentally created transcondylar humerus fractures in cadaveric elbows, which are the most frequently observed humeral fractures in the elderly, and compared it with the rigidity achieved by 3 conventional fixation methods:an LCP reconstruction plate 3.5 using a locking mechanism (Group II), a conventional reconstruction plate 3.5 (CRP) with a cannulated cancellous screw (Group III), and a CRP with 2 cannulated cancellous screws (CS) in a crisscross orientation (Group IV). In the axial loading test, the mean failure loads were:Group I, 98.9+/-32.6;Group II, 108.5+/-27.2;Group III, 50.0+/-7.5;and Group IV, 34.5+/-12.2 (N). Group I fixations failed at a significantly higher load than those of Groups III and IV (p0.05). In the extension loading test, the mean failure loads were:Group I, 34.0+/-12.4;Group II, 51.0+/-14.8;Group III, 19.3+/-6.0;and Group IV, 14.7+/-3.1 (N). Group IV fixations showed a significantly lower failure load than those of Group I (p0.05). The fixation rigidities against mechanical loading by the ONI plate and LCP plate were comparable. These results suggested that an ONI system might be superior to the CRP and CS method, and comparable to the LCP method in terms of fixation rigidity for distal humerus fractures.

Keywords distal humerus fracture biomechanics internal fixation elderly
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2010-04
Volume volume64
Issue issue2
Publisher Okayama University Medical School
Start Page 115
End Page 120
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 20424666
Web of Science KeyUT 000276996900005
FullText URL fulltext.pdf
Author Inai, Ryota| Nakahara, Ryuichi| Morimitsu, Yusuke| Akagi, Noriaki| Marukawa, Youhei| Matsushita, Toshi| Tanaka, Takashi| Tada, Akihiro| Hiraki, Takao| Nasu, Yoshihisa| Nishida, Keiichiro| Ozaki, Toshifumi| Kanazawa, Susumu|
Keywords Osteoporosis Bone density Tomography (x-ray computed) X-ray microtomography
Published Date 2021-01-28
Publication Title European Radiology Experimental
Volume volume4
Issue issue1
Publisher Springer nature
Start Page 4
ISSN 2509-9280
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © The Author(s). 2020
File Version publisher
PubMed ID 31993864
DOI 10.1186/s41747-019-0135-0
Web of Science KeyUT 000705622700001
Related Url isVersionOf https://doi.org/10.1186/s41747-019-0135-0
JaLCDOI 10.18926/AMO/31823
FullText URL fulltext.pdf
Author Tanaka, Masato| Nakanishi, Kazuo| Sugimoto, Yoshihisa| Misawa, Haruo| Takigawa, Tomoyuki| Nishida, Keiichiro| Ozaki, Toshifumi|
Abstract

Scoliosis is a common clinical manifestation of Rett syndrome, a neurodevelopmental disorder that almost exclusively affects females. The spinal curve in patients with Rett syndrome is typically a long C curve of a neuromuscular type. As the onset of the scoliosis is very early and shows rapid progression, early surgical intervention has been recommended to prevent a life-threatening collapsing spine syndrome. However, there are high perioperative risks in Rett syndrome patients who undergo spinal surgery, such as neurological compromise and respiratory dysfunction due to rigid spinal curve. We herein report the surgical result of treating severe rapid progressive thoracic scoliosis in a 16-year-old girl with Rett syndrome. Posterior segmental pedicle screw fixation was performed from T1 to L3 using a computer-assisted technique. Post-operative radiography demonstrated a good correction of the curve in both the sagittal and coronal alignment. There were no postoperative complications such as neurological compromise. The patient had maintained satisfactory spinal balance as of the 3-year follow-up examination.

Keywords Rett syndrome scoliosis computer navigation-assisted surgery segmental pedicle screw fixation
Amo Type Case Report
Publication Title Acta Medica Okayama
Published Date 2009-12
Volume volume63
Issue issue6
Publisher Okayama University Medical School
Start Page 373
End Page 377
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 20035294
Web of Science KeyUT 000273145900009
JaLCDOI 10.18926/AMO/30749
FullText URL fulltext.pdf
Author Komiyama, Takamitsu| Nishida, Keiichiro| Yorimitsu, Masanori| Doi, Hideyuki| Miyazawa, Shinichi| Kitamura, Ai| Yoshida, Aki| Nasu, Yoshihisa| Abe, Nobuhiro| Ozaki, Toshifumi|
Abstract Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p<0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR.
Keywords spontaneous hypertensive rats insulin like growth factor-1 vascular endothelial growth factor ossification disturbance osteonecrosis
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 2006-06
Volume volume60
Issue issue3
Publisher Okayama University Medical School
Start Page 141
End Page 148
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 16838042
Web of Science KeyUT 000238503600001
FullText URL JOS_015_Fulltext.pdf JOS_015_Figures.pptx
Author Okazaki, Yuki| Furumatsu, Takayuki| Kamatsuki, Yusuke| Nishida, Keiichiro| Nasu, Yoshihisa| Nakahara, Ryuichi| Saito, Taichi| Ozaki, Toshifumi|
Published Date 2020-03-26
Publication Title Journal of Orthopaedic Science
Volume volume26
Issue issue2
Publisher Elsevier
Start Page 230
End Page 236
ISSN 09492658
NCID AA11052566
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2020 The Japanese Orthopaedic Association.
File Version author
PubMed ID 32223991
DOI 10.1016/j.jos.2020.02.015
Related Url isVersionOf https://doi.org/10.1016/j.jos.2020.02.015
JaLCDOI 10.18926/AMO/31296
FullText URL fulltext.pdf
Author Takeuchi, Kazuhiro| Inoue, Hajime| Yokoyama, Yoshiki| Senda, Masuo| Ota, Yusuke| Abe, Nobuhiro| Nishida, Keiichiro|
Abstract

We studied the magnetic resonance imaging (MRI) of 120 knees in 86 rheumatoid arthritis (RA) patients and of 14 unaffected knees in 12 control cases. We also developed a scoring system as a quantitative analysis method. We divided the MRI into 10 items, and classified the severity of the symptoms into 4 grades (score 0 to 3). The average total score increased according to the radiographic grade. Soft tissue lesions were clearly detected, even in the early stages of RA. Items such as synovial proliferation showed a high score even in the early stages, suggesting that it was the initial symptom of RA. The score also showed a correlation with the inflammatory signs. These results suggest that this scoring system is very sensitive and yields a good reflection of RA activity. We demonstrated that this system is simple and convenient for routine diagnostic use. We further demonstrated that it is useful for following the advancement of RA and for evaluating the response to treatment.

Keywords rheumatoid arthritis magnetic resonance imaging scoring system synovial membrane
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 1998-08
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 211
End Page 224
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 9781272
Web of Science KeyUT 000075623600006
JaLCDOI 10.18926/AMO/32000
FullText URL fulltext.pdf
Author Matsuo, Masatsugu| Nishida, Keiichiro| Yoshida, Aki| Murakami, Takuro| Inoue, Hajime|
Abstract

To clarify the involvement of the caspase family in the pathway of NO-induced chondrocyte apoptosis, osteoarthritis (OA) cartilage obtained from 8 patients undergoing total hip arthroplasty were used for histopathological study. Cartilage samples taken from non-fibrillated areas of femoral head resected during surgery for femoral neck fracture were used for comparison. DNA fragmentation of chondrocytes was detected by the nick end-labeling (TUNEL) method. Apoptosis was further confirmed by transmission electron microscopy. The distributions of nitrotyrosine (NT), caspase-3, and -9 were examined immunohistochemically. The populations of apoptotic as well as NT-, caspase-3-, and -9-positive cells were quantified by counting the number of cells in the superficial, middle, and deep layers, respectively. The TUNEL-positive cells were observed primarily in superficial proliferating chondrocytes, clustering chondrocytes, and deep-layer chondrocytes of OA cartilage. Few positive cells were seen in the proliferating chondrocytes in the middle layer. Positive reactions for caspase-3 and -9 were observed in chondrocytes in similar areas. Histological OA grade showed significant correlations with the mean populations of apoptotic chondrocytes (% apoptosis) over the 3 areas. The populations of NT-positive cells (% NT) over the same areas also showed significant correlation with OA grade. Positivity for caspase-3 closely correlated with the OA grade, % apoptosis and %NT. It was concluded that caspase-3 and -9 could play a role in NO-induced chondrocyte apoptosis in OA cartilage.

Keywords apoptosis caspase nitric oxide osteoarthritis chondrocyte
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 2001-12
Volume volume55
Issue issue6
Publisher Okayama University Medical School
Start Page 333
End Page 340
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 11779095
Web of Science KeyUT 000172838400003
JaLCDOI 10.18926/AMO/30790
FullText URL fulltext.pdf
Author Nanba, Yoshifumi| Nishida, Keiichiro| Yoshikawa, Takeshi| Sato, Toru| Inoue, Hajime| Kuboki, Yoshinori|
Abstract

The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.

Keywords chondrocalcinosis osteoarthritis osteonection Mankin's histologic-histochemical grading calcification
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 1997-10
Volume volume51
Issue issue5
Publisher Okayama University Medical School
Start Page 239
End Page 243
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 9359920
Web of Science KeyUT A1997YD65300001
FullText URL fulltext.pdf
Author Tsuji, Hironori| Tetsunaga, Tomoko| Tetsunaga, Tomonori| Misawa, Haruo| Oda, Yoshiaki| Takao, Shinichiro| Nishida, Keiichiro| Ozaki, Toshifumi|
Keywords caregiver burden chronic pain fear-avoidance model self-efficacy Zarit Burden Interview
Published Date 2022-09-30
Publication Title Medicine
Volume volume101
Issue issue39
Publisher Lippincott Williams & Wilkins
Start Page e30802
ISSN 0025-7974
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2022 the Author(s).
File Version publisher
PubMed ID 36181114
DOI 10.1097/MD.0000000000030802
Web of Science KeyUT 000862039900121
Related Url isVersionOf https://doi.org/10.1097/MD.0000000000030802
JaLCDOI 10.18926/AMO/31338
FullText URL fulltext.pdf
Author Takagoshi, Hidekazu| Hashizume, Hiroyuki| Nishida, Keiichiro| Masaoka, shunji| Asahara, Hiroshi| Inoue, Hajime|
Abstract

The fibrous components of the metacarpophalangeal (MP) joint including the palmar plate, the collateral ligament and the dorsal plate were studied with particular attention paid to the fibrous structure of the fibrous tendon sheath and the deep transverse metacarpal ligament. The tough fibrillar structure around the MP joint, especially the force nucleus, consisted of three types of mixed fibers: the fibrous tendon sheath of the A1 pulley, the deep transverse metacarpal ligament, and the palmar plate. The tendon sheath was located on the ulnar side in the index and middle fingers, on the central position in the ring finger, and on the radial side in the little finger. These fibrous connections among the fingers formed a transverse arch in the hand. The palmar plate of the MP joint was relatively rigid and appears to function as a cushion when flexed. A fold-like protrusion of the synovial layer of the palmar plate of the MP joint had a meniscoid function, which was larger than that of the proximal interphalangeal joint. The capsule of the MP joint was thicker at the dorsal area, forming a dorsal plate, which is a sliding floor of the extensor mechanism and has a meniscoid function for joint congruity. The main lateral stabilizer consisted of collateral ligaments and accessory collateral ligaments anchored to the palmar plate. These structures act together as a "phalangeal cuff", connecting the proximal phalanx to the metacarpal head and sta

Keywords metacarpophalangeal joint collagen framework
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 1998-02
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 19
End Page 26
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 9548990
Web of Science KeyUT 000072264100003
JaLCDOI 10.18926/AMO/47262
FullText URL 65_6_369.pdf
Author Terada, Chuji| Yoshida, Aki| Nasu, Yoshihisa| Mori, Shuji| Tomono, Yasuko| Tanaka, Masato| Takahashi, Hideo K.| Nishibori, Masahiro| Ozaki, Toshifumi| Nishida, Keiichiro|
Abstract We investigated the expression and localization of high-mobility group box chromosomal protein-1 (HMGB-1) in human osteoarthritic (OA) cartilage in relation to the histopathological grade of cartilage destruction, and examined the role of HMGB-1 in the regulation of proinflammatory cytokine expression in chondrocytes. An immunohistochemical study demonstrated that total HMGB-1-positive cell ratios increase as the Osteoarthritis Research Society International (OARSI) histological grade increased. The population of cytoplasmic HMGB-1-positive chondrocytes was especially increased in the deep layers of higher-grade cartilage. The ratios and localization of receptors for advanced glycation end products (RAGE) expression by chondrocytes in Grade 2, 3, and 4 were significantly higher than those in Grade 1. In vitro stimulation with IL-1β, but not TNFα, significantly upregulated the expression of HMGB-1 mRNA by human OA chondrocytes. Both IL-1β and TNFα promoted the translocation of HMGB-1 from nuclei to cytoplasm. IL-1β and TNFα secretions were stimulated at higher levels of HMGB-1. The results of our study suggest the involvement of HMGB-1 in the pathogenesis of cartilage destruction in OA.
Keywords HMGB-1 RAGE chondrocyte osteoarthritis cartilage
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2011-12
Volume volume65
Issue issue6
Publisher Okayama University Medical School
Start Page 369
End Page 377
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2011 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22189477
Web of Science KeyUT 000298516900003
JaLCDOI 10.18926/AMO/31711
FullText URL fulltext.pdf
Author Kosaka, Motohiro| Horiuchi, Kanji| Nishida, Keiichiro| Taguchi, Takehito| Murakami, Takuro| Ohtsuka, Aiji|
Abstract

The celiac and mesenteric arterial system including the left gastric, splenic, common hepatic, and superior mesenteric arteries shows various types of origins, courses, ramifications and anastomoses. In order to explain the various expressions of this system, we have proposed a typological model, in which celiacomesenteric arteries develop as paired or bilaterally symmetrical primordial vessels originated from the anterior aspect of the aorta, and these vessels anastomose each other with longitudinal and horizontal pathways. Here, we report 3 unusual cases characterized by arterial rings, formed by the left gastric, left accessory hepatic, proper hepatic, anterior pancreaticoduodenal, and dorsal pancreatic arteries. The dorsal pancreatic and anterior pancreaticoduodenal arteries are located to the right and left of the embryonic pancreas developing in the dorsal mesentery, respectively. Such hepatopancreatic arterial rings simultaneously containing right and left elements can only be explained using our typological model, in which the concept of paired arteries or bilateral symmetry is introduced.

Keywords arterial variation celiac trunk superior mesenteric artery typology bilateral symmetry
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 2002-10
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 245
End Page 253
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 12530508
Web of Science KeyUT 000178668100005
JaLCDOI 10.18926/AMO/66673
FullText URL 78_1_071.pdf
Author Kaneda, Daisuke| Nishida, Keiichiro| Nasu, Yoshihisa| Nakahara, Ryuichi| Harada, Ryozo| Hotta, Yoshifumi| Naniwa, Shuichi| Ozaki, Toshifumi|
Abstract We retrospectively investigated the mid-term outcomes of arthroplasty using the AVANTA silicone implant for thumb metacarpophalangeal (MCP) joints with boutonniere deformity in patients with rheumatoid arthritis (RA). This study involved 36 thumbs of 33 RA patients with a mean follow-up period of 5.1 years (range, 2.0-13.3). Postoperatively, the mean extension was significantly increased and the mean flexion was significantly decreased (p<0.001, p<0.001, respectively), resulting in the mean arc of range of motion (ROM) shifting in the direction of extension after surgery. Implant fracture was observed in 10 thumbs (28%), and 4 of these (11%) underwent revision surgery. The survivorship with implant fracture and revision surgery as endpoints were 73.4% and 91.8% at 5 years, respectively. The preoperative arc of ROM and the postoperative flexion range of the implant-fracture group were significantly greater than those in the no-implant-fracture group (p=0.039, 0.034, respectively). These results suggest the importance of patient education and careful rehabilitation to prevent excessive flexion. Overall, the AVANTA silicone implant showed a relatively high rate of implant fracture at our institute.
Keywords AVANTA silicone implant boutonniere deformity implant fracture thumb metacarpophalangeal joint arthroplasty rheumatoid arthritis
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2024-02
Volume volume78
Issue issue1
Publisher Okayama University Medical School
Start Page 71
End Page 78
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders Copyright Ⓒ 2024 by Okayama University Medical School
File Version publisher
Refereed True
Author 西田 圭一郎|
Published Date 1995-09-30
Publication Title
Content Type Thesis or Dissertation
JaLCDOI 10.18926/AMO/30778
FullText URL fulltext.pdf
Author Kinugasa, Kiyoto| Hashizume, Hiroyuki| Nishida, Keiichiro| Shigeyama, Yukio| Inoue, Hajime|
Abstract

The results of the histological examinations of specimens of the tenosynovium of the flexor tendon, the epineurium and the transverse carpal ligament from two groups of Japanese patients with carpal tunnel syndrome (idiopathic and hemodialysis) were compared. Amyloid deposits, positively identified as β2-microglobulin, appeared in all patients in the long-term hemodialysis group, but in no patients in the idiopathic group. Although the pathogenesis differed between the two groups, both resulted in nerve compression in the carpal tunnel. Therefore, surgical release is considered beneficial for both groups.

Keywords carpal tunnel syndrome histopathology clinical results idiopathic hemodialysis
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 1997-04
Volume volume51
Issue issue2
Publisher Okayama University Medical School
Start Page 63
End Page 70
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 9142342
Web of Science KeyUT A1997WX19600002
JaLCDOI 10.18926/AMO/57957
FullText URL 74_1_77.pdf
Author Endo, Hirosuke| Akazawa, Hirofumi| Yashiro, Masato| Yamada, Kazuki| Sanki, Tomoaki| Tetsunaga, Tomonori| Nishida, Keiichiro| Furumatsu, Takayuki| Ozaki, Toshifumi|
Abstract Idiopathic chondrolysis of the hip (ICH), a very rare disorder of unknown etiology, occurs mainly in female adolescents. Characterized by pain, limp, stiffness and radiological narrowing joint space from the rapid destruction of the articular cartilage, ICH sometimes results in ankyloses. We present the case of a 10-year-old girl diagnosed with ICH based on arthroscopic inspection and synovium biopsy. The femoral deformity appeared gradually, like a cam-type femoroacetabular impingement. She was treated with intensive rehabilitation and immunosuppressive drug. We later performed an arthroscopic bumpectomy for residual symptoms. She achieved a favorable outcome as a 15-year-old at the latest follow-up.
Keywords idiopathic chondrolysis hip joint medication bump arthroscopy
Amo Type Case Report
Publication Title Acta Medica Okayama
Published Date 2020-02
Volume volume74
Issue issue1
Publisher Okayama University Medical School
Start Page 77
End Page 81
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2020 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 32099253
Web of Science KeyUT 000516606200012
NAID 120006795624
JaLCDOI 10.18926/AMO/30753
FullText URL fulltext.pdf
Author Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji|
Abstract

We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.

Keywords colloidal iron microwave histochemistry transmission electron microscopy
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 2006-02
Volume volume60
Issue issue1
Publisher Okayama University Medical School
Start Page 59
End Page 64
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 16508690
Web of Science KeyUT 000235538900007
JaLCDOI 10.18926/AMO/32287
FullText URL fulltext.pdf
Author Shibahara, Motomi| Nishida, Keiichiro| Asahara, Hiroshi| Yoshikawa, Takeshi| Mitani, Shigeru| Kondo, Yoichi| Inoue, Hajime|
Abstract

We investigated the presence of osteocyte apoptosis in the necrotic trabeculae of the femoral head of spontaneously hypertensive rat (SHR) using the in situ nick end labeling (TUNEL) method and transmission electron microscopy. The occurrence of osteonecrosis and ossification disturbance was significantly higher in SHR compared with Wistar Kyoto (WKY) rats, and Wistar (WT) rats used as control animals (P < 0.01). A high population of TUNEL positive osteocytes was detected mainly in 10- and 15-week-old SHRs. Sectioned examination of the femoral head of SHRs and WKY rats by electron microscopy revealed apoptotic cell appearances such as aggregation of chromatin particles and lipid formation. In contrast, a positive reaction was significantly lower in osteocytes in the femoral heads of WT rats (P < 0.01). Our results indicate that apoptosis forms an important component of the global pathologic process affecting the femoral head of SHR, which leads to osteonecrosis in this region.

Keywords apoptosis spontaneously hypertensive rat osteonecrosis of the femoral head
Amo Type Article
Publication Title Acta Medica Okayama
Published Date 2000-04
Volume volume54
Issue issue2
Publisher Okayama University Medical School
Start Page 67
End Page 74
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
File Version publisher
Refereed True
PubMed ID 10806527
Web of Science KeyUT 000086735900003