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ID 59969
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Cai, Hongmei Institute of Plant Science and Resources, Okayama University
Huang, Sheng Institute of Plant Science and Resources, Okayama University
Che, Jing Institute of Plant Science and Resources, Okayama University
Yamaji, Naoki Institute of Plant Science and Resources, Okayama University
Ma, Jian Feng Institute of Plant Science and Resources, Okayama University ORCID Kaken ID publons researchmap
Abstract
In order to respond to fluctuating zinc (Zn) in the environment, plants must have a system to control Zn homeostasis. However, how plants maintain an appropriate level of Zn during their growth and development is still poorly understood. In this study, we found that OsHMA3, a tonoplast-localized transporter for Zn/Cd, plays an important role in Zn homeostasis in rice. Accessions with the functional allele of OsHMA3 showed greater tolerance to high Zn than those with the non-functional allele based on root elongation test. A 67Zn-labeling experiment showed that accessions with loss of function of OsHMA3 had lower Zn accumulation in the roots but similar concentrations in the shoots compared with functional OsHMA3 accessions. When exposed to Zn-free growing medium, the concentration in the root cell sap was rapidly decreased in accessions with functional OsHMA3, but less dramatic changes were observed in non-functional accessions. A mobility experiment showed that more Zn in the roots was translocated to the shoots in accessions with functional OsHMA3. Higher expression levels of OsZIP4, OsZIP5, OsZIP8, and OsZIP10 were found in the roots of accessions with functional OsHMA3 in response to Zn deficiency. Taken together, our results indicate that OsHMA3 plays an important role in rice roots in both Zn detoxification and storage by sequestration into the vacuoles, depending on Zn concentration in the environment.
Keywords
OsHMA3
vacuolar sequestration
ZIP transporter
Zn distribution
Zn root-to-shoot mobility
Zn tolerance
Published Date
2019-05-06
Publication Title
Journal of Experimental Botany
Volume
volume70
Issue
issue10
Publisher
Oxford University Press
Start Page
2717
End Page
2725
ISSN
0022-0957
NCID
AA00697526
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s)
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DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1093/jxb/erz091
Citation
Cai, H. et. al. The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice. Journal of Experimental Botany 70(10) p.2717–2725 (2019)
Funder Name
Ministry of Education, Culture, Sports, Science and Technology
助成番号
16H06296