start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2006
dt-pub=20060803
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Simultaneous gene transfer of bone morphogenetic protein (BMP)-2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p><b>Background:</b> Transcutaneous in vivo electroporation is expected to be an effective gene-transfer method for promoting bone regeneration using the BMP-2 plasmid vector. To promote enhanced osteoinduction using this method, we simultaneously transferred cDNAs for BMP-2 and BMP-7, as inserts in the non-viral vector pCAGGS.<br />
<b>Methods:</b> First, an in vitro study was carried out to confirm the expression of BMP-2 and BMP-7 following the double-gene transfer. Next, the individual BMP-2 and BMP-7 plasmids or both
together were injected into rat calf muscles, and transcutaneous electroporation was applied 8 times at 100 V, 50 msec.<br />
<b>Results:</b> In the culture system, the simultaneous transfer of the BMP-2 and BMP-7 genes led to a much higher ALP activity in C2C12 cells than did the transfer of either gene alone. In vivo, ten days after the treatment, soft X-ray analysis showed that muscles that received both pCAGGS-BMP-2 and pCAGGS-BMP-7 had better-defined opacities than those receiving a single gene. Histological examination showed advanced ossification in calf muscles that received the double-gene transfer.
BMP-4 mRNA was also expressed, and RT-PCR showed that its level increased for 3 days in a timedependent manner in the double-gene transfer group. Immunohistochemistry confirmed that BMP-
4-expressing cells resided in the matrix between muscle fibers.<br />
<b>Conclusion:</b> The simultaneous transfer of BMP-2 and BMP-7 genes using in vivo electroporation induces more rapid bone formation than the transfer of either gene alone, and the increased
expression of endogenous BMP-4 suggests that the rapid ossification is related to the induction of
BMP-4.</p>
en-copyright=
kn-copyright=

en-aut-name=KawaiMariko
en-aut-sei=Kawai
en-aut-mei=Mariko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=BesshoKazuhisa
en-aut-sei=Bessho
en-aut-mei=Kazuhisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=MaruyamaHiroki
en-aut-sei=Maruyama
en-aut-mei=Hiroki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MiyazakiJun-ichi
en-aut-sei=Miyazaki
en-aut-mei=Jun-ichi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YamamotoToshio
en-aut-sei=Yamamoto
en-aut-mei=Toshio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University

affil-num=3
en-affil=
kn-affil=Division of Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences

affil-num=4
en-affil=
kn-affil=Division of Stem Cell Regulation Research, Osaka University Medical School

affil-num=5
en-affil=
kn-affil=Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
END